Publikationen

2018

  • Seifert, E. and Tode, J. and Pielen, A. and Theisen-Kunde, D. and Framme, C. and Roider, J. and Miura, Y. and Birngruber, R. and Brinkmann, R.: Selective retina therapy: toward an optically controlled automatic dosing. J Biomed Opt, no. 23, pp. 1-12, 2018
    BibTeX Link Link
    @article{seifert2018,
       author = {Seifert, E. and Tode, J. and Pielen, A. and Theisen-Kunde, D. and Framme, C. and Roider, J. and Miura, Y. and Birngruber, R. and Brinkmann, R.},
       title = {Selective retina therapy: toward an optically controlled automatic dosing},
       journal = {J Biomed Opt},
       volume = {23},
       number = {11},
       pages = {1-12},
       ISSN = {1560-2281 (Electronic)
    1083-3668 (Linking)},
       DOI = {10.1117/1.JBO.23.11.115002},   
    keywords = {algorithm, lasers in medicine, ophthalmology, retinal pigment epithelium, selective retina therapy, selectivity}
       year = {2018},
       type = {Journal Article}
    }
    
  • Jonas Smits and Mouloud Ourak and Andy Gijbels and Laure Esteveny and Gianni Borghesan and Laurent Schoevaerdts and K. Willekens P. Stalmans E. Lankenau Hinnerk Schulz-Hildebrandt and Gereon Hüttmann and Dominiek Reynaerts and Emmanuel B. Vander Poorten: Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation. 2018 IEEE International Conference on Robotics and Automation (ICRA), pp. 129-134, 2018
    BibTeX Link Link
    @inproceedings{Smits2018,
    title = {Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation},
    author = {Jonas Smits and Mouloud Ourak and Andy Gijbels and Laure Esteveny and Gianni Borghesan and Laurent Schoevaerdts and K. Willekens P. Stalmans E. Lankenau Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Dominiek Reynaerts and Emmanuel B. Vander Poorten},
    
    doi = {10.1109/ICRA.2018.8460983},
    year = {2018},
    date = {2018-09-20},
    journal = {2018 IEEE International Conference on Robotics and Automation (ICRA)},
    pages = {129-134},
    abstract = {Retinal Vein Occlusion is a common retinal vascular disorder which can cause severe loss of vision. Retinal vein cannulation followed by injection of an anti-coagulant into the affected vein is a promising treatment. However, given the scale and fragility of the surgical workfield, this procedure is considered too high-risk to perform manually. A first successful robot-assisted procedure has been demonstrated. Even though successful, the procedure remains extremely challenging. This paper aims at providing a solution for the limited perception of instrument-tissue interaction forces as well as depth estimation during retinal vein cannulation. The development of a novel combined force and distance sensing cannulation needle relying on Fiber Bragg grating (FBG) and Optical Coherence Tomography (OCT) A-scan technology is reported. The design, the manufacturing process, the calibration method, and the experimental characterization of the produced sensor are discussed. 
    The functionality of the combined sensing modalities and the real-time distance estimation algorithm are validated respectively on in-vitro and ex-vivo models.},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Hinnerk Schulz-Hildebrandt and Tom Pfeiffer and Tim Eixmann and Sabrina Lohmann and Martin Ahrens and Josua Rehra and Wolfgang Draxinger and Peter König and Robert Huber and Gereon Hüttmann: High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography. Optics Letters, no. 43, pp. 4386-4389, 2018
    BibTeX Link
    @article{Schulz-Hildebrandt2018b,
    title = {High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography},
    author = {Hinnerk Schulz-Hildebrandt /Tom Pfeiffer /Tim Eixmann /Sabrina Lohmann /Martin Ahrens /Josua Rehra /Wolfgang Draxinger /Peter K\"{o}nig /Robert Huber and Gereon H\"{u}ttmann},
    url = {https://www.osapublishing.org/ol/abstract.cfm?uri=ol-43-18-4386#Abstract},
    
    year = {2018},
    date = {2018-09-05},
    journal = {Optics Letters},
    volume = {43},
    number = {18},
    pages = {4386-4389},
    abstract = {We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 μm was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in spirals for scanning the sample, the beam quality remains constant over the entire field of view with a diameter of 0.8 mm. The scanning frequency was adjusted to 1.22 kHz for use with a 3.28 MHz Fourier domain mode locked OCT system. Densely sampled volumes have been imaged at a rate of 6 volumes per second.
    
    © 2018 Optical Society of America},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Pfeiffer, Tom / Petermann, Markus /Draxinger, Wolfgang / Jirauschek, Christian and Huber, Robert: Ultra low noise Fourier domain mode locked laser for high quality magahertz optical coherence tomography. Biomedical Optics Express, no. 9, pp. 4130-4148, 2018
    BibTeX Link
    @article{Pfeiffer,
       author = {Pfeiffer, Tom / Petermann, Markus /Draxinger, Wolfgang / Jirauschek, Christian and Huber, Robert},
       title = {Ultra low noise Fourier domain mode locked laser for high quality magahertz optical coherence tomography},
       journal = {Biomedical Optics Express},
       volume = {9},
       number = {9},
       pages = {4130-4148},
        DOI = {10.1364/BOE.9.004130},
    url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052736688&doi=10.1364%2fBOE.9.004130&partnerID=40&md5=1ed100666a2d806f51b68e70ba13808c}
       year = {2018},
       type = {Journal Article}
    }
    
  • Hendrik Spahr and Clara Pfäffle and Peter Koch and Helge Sudkamp and Gereon Hüttmann and Dierck Hillmann: Interferometric detection of 3D motion using computational subapertures in optical coherence tomography. Opt. Express, no. 26, pp. 18803-18816, OSA, Jul, 2018
    BibTeX Link Link
    @article{Spahr:18,
    author = {Hendrik Spahr, Clara Pfäffle, Peter Koch, Helge Sudkamp, Gereon Hüttmann und Dierck Hillmann},
    journal = {Opt. Express},
    keywords = {Funktion, Fullfield},
    number = {15},
    pages = {18803--18816},
    publisher = {OSA},
    title = {Interferometric detection of 3D motion using computational subapertures in optical coherence tomography},
    volume = {26},
    month = {Jul},
    year = {2018},
    url = {http://www.opticsexpress.org/abstract.cfm?URI=oe-26-15-18803},
    doi = {10.1364/OE.26.018803},
    abstract = {Doppler optical coherence tomography (OCT) quantifies axial motion with high precision, whereas lateral motion cannot be detected by a mere evaluation of phase changes. This problem was solved by the introduction of three-beam Doppler OCT, which, however, entails a high experimental effort. Here, we present the numerical analogue to this experimental approach. Phase-stable complex-valued OCT datasets, recorded with full-field swept-source OCT, are filtered in the Fourier domain to limit imaging to different computational subapertures. These are used to calculate all three components of the motion vector with interferometric precision. As known from conventional Doppler OCT for axial motion only, the achievable accuracy exceeds the actual imaging resolution by orders of magnitude in all three dimensions. The feasibility of this method is first demonstrated by quantifying micro-rotation of a scattering sample. Subsequently, a potential application is explored by recording the 3D motion vector field of tissue during laser photocoagulation in ex-vivo porcine retina.},
    }
    
    
  • Sudkamp, Helge and Hillmann, Dierck and Koch, Peter and Endt, Malte vom and Spahr, Hendrik and Münst, Michael and Pfäffle, Clara and Birngruber, Reginald and Hüttmann, Gereon: Simple approach for aberration-corrected OCT imaging of the human retina. Optics Letters, no. 43, pp. 4224, 2018
    BibTeX Link
    @article{Sudkamp2018a,
       author = {Sudkamp, Helge and Hillmann, Dierck and Koch, Peter and Endt, Malte vom and Spahr, Hendrik and Münst, Michael and Pfäffle, Clara and Birngruber, Reginald and Hüttmann, Gereon},
       title = {Simple approach for aberration-corrected OCT imaging of the human retina},
       journal = {Optics Letters},
       volume = {43},
       number = {17},
       pages = {4224},
       ISSN = {0146-9592
    1539-4794},
       DOI = {10.1364/ol.43.004224},
       year = {2018},
       type = {Journal Article},
       keyword = {Retome}
    }
    
  • Ourak, M. and Smits, J. and Esteveny, L. and Borghesan, G. and Gijbels, A. and Schoevaerdts, L. and Douven, Y. and Scholtes, J. and Lankenau, E. and Eixmann, T. and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and Kozlovszky, M. and Kronreif, G. and Willekens, K. and Stalmans, P. and Faridpooya, K. and Cereda, M. and Giani, A. and Staurenghi, G. and Reynaerts, D. and Vander Poorten, E. B.: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. International Journal of Computer Assisted Radiology and Surgery, no. 13, pp. 1-9, 2018
    BibTeX Link Link
    @article{Ourak2018,
    title = {Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation},
    author = {Ourak, M. and Smits, J. and Esteveny, L. and Borghesan, G. and Gijbels, A. and Schoevaerdts, L. and Douven, Y. and Scholtes, J. and Lankenau, E. and Eixmann, T. and Schulz-Hildebrandt, Hinnerk and H\"{u}ttmann, Gereon and Kozlovszky, M. and Kronreif, G. and Willekens, K. and Stalmans, P. and Faridpooya, K. and Cereda, M. and Giani, A. and Staurenghi, G. and Reynaerts, D. and Vander Poorten, E. B.},
    
    doi = {10.1007/s11548-018-1829-0},
    year = {2018},
    date = {2018-07-28},
    journal = {International Journal of Computer Assisted Radiology and Surgery},
    volume = {13},
    number = {107},
    pages = {1-9},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Evers, M. and Salma, N. and Osseiran, S. and Casper, M. and Birngruber, R. and Evans, C. L. and Manstein, D.: Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM. Scientific Reports, no. 8, 2018
    BibTeX Link
    @article{Evers2018,
       author = {Evers, M. and Salma, N. and Osseiran, S. and Casper, M. and Birngruber, R. and Evans, C. L. and Manstein, D.},
       title = {Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM},
       journal = {Scientific Reports},
       volume = {8},
       number = {1},
       DOI = {10.1038/s41598-018-27093-x},
       
       year = {2018},
       type = {Journal Article}
    }
    
  • Miura, Yoko: Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo. in Mouse Retinal Phenotyping: Methods and Protocols, pp. 73-88, Springer New York, New York, NY, 2018
    BibTeX Link
    @inbook{Miura2018,
       author = {Miura, Yoko},
       title = {Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo},
       booktitle = {Mouse Retinal Phenotyping: Methods and Protocols},
       editor = {Tanimoto, Naoyuki},
       publisher = {Springer New York},
       address = {New York, NY},
       pages = {73-88},
       ISBN = {978-1-4939-7720-8},
       url={https://doi.org/10.1007/978-1-4939-7720-8_5},
       year = {2018},
       type = {Book Section}
    }
    
  • Tode, J. and Richert, E. and Koinzer, S. and Klettner, A. and von der Burchard, C. and Brinkmann, R. and Lucius, R. and Roider, J.: Thermal Stimulation of the Retina Reduces Bruch`s Membrane Thickness in Age Related Macular Degeneration Mouse Models. Transl Vis Sci Technol, no. 7, pp. 2, 2018
    BibTeX Link
    @article{Tode2018,
       author = {Tode, J. and Richert, E. and Koinzer, S. and Klettner, A. and von der Burchard, C. and Brinkmann, R. and Lucius, R. and Roider, J.},
       title = {Thermal Stimulation of the Retina Reduces Bruch's Membrane Thickness in Age Related Macular Degeneration Mouse Models},
       journal = {Transl Vis Sci Technol},
       volume = {7},
       number = {3},
       pages = {2},
       ISSN = {2164-2591 (Print)
    2164-2591 (Linking)}, 
       url = {https://www.ncbi.nlm.nih.gov/pubmed/29736323},
       year = {2018},
       type = {Journal Article}
    }
    
  • Evers, M. and Ha, L. and Casper, M. and Welford, D. and Kositratna, G. and Birngruber, R. and Manstein, D.: Assessment of skin lesions produced by focused, tunable, mid-infrared chalcogenide laser radiation. Lasers in Surgery and Medicine, 2018
    BibTeX Link
    @article{Evers2018
       author = {Evers, M. and Ha, L. and Casper, M. and Welford, D. and Kositratna, G. and Birngruber, R. and Manstein, D.},
       title = {Assessment of skin lesions produced by focused, tunable, mid-infrared chalcogenide laser radiation},
       journal = {Lasers in Surgery and Medicine},
       DOI = {10.1002/lsm.22935},
      
       year = {2018},
       type = {Journal Article}
    }
    
  • Richert, Elisabeth and Koinzer, Stefan and Tode, Jan and Schlott, Kerstin and Brinkmann, Ralf and Hillenkamp, Jost and Klettner, Alexa and Roider, Johann: Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy. Investigative Ophthalmology & Visual Science, no. 59, pp. 1323-1331, 2018
    BibTeX Link
    @article{Richert2018,
       author = {Richert, Elisabeth and Koinzer, Stefan and Tode, Jan and Schlott, Kerstin and Brinkmann, Ralf and Hillenkamp, Jost and Klettner, Alexa and Roider, Johann},
       title = {Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {59},
       number = {3},
       pages = {1323-1331},
       ISSN = {1552-5783},
       DOI = {10.1167/iovs.17-23163},
       
       year = {2018},
       type = {Journal Article}
    }
    
  • Kern, K. and Mertineit, C. L. and Brinkmann, R. and Miura, Y.: Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells. Exp Eye Res, no. 170, pp. 117-126, 2018
    BibTeX Link
    @article{Miura2018,
       author = {Kern, K. and Mertineit, C. L. and Brinkmann, R. and Miura, Y.},
       title = {Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells},
       journal = {Exp Eye Res},
       volume = {170},
       pages = {117-126},
       ISSN = {1096-0007 (Electronic)
    0014-4835 (Linking)},
       DOI = {10.1016/j.exer.2018.02.013},
       year = {2018},
       type = {Journal Article}
    }
    
  • Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Reginald Birngruber and Dieter Manstein and Gereon Hüttmann: Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation). Proc.SPIE, in Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, no. 10467, 2018
    BibTeX Link Link
    @conference{Casper2018,
    title = {Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation)},
    author = {Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Reginald Birngruber and Dieter Manstein and Gereon H\"{u}ttmann},
    url = {https://doi.org/10.1117/12.2292005},
    doi = {10.1117/12.2292005},
    year = {2018},
    date = {2018-03-14},
    booktitle = {Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018},
    journal = {Proc.SPIE},
    volume = {10467},
    abstract = {The ability to image the physiology of microvasculature with high spatial resolution in three dimensions while investigating structural changes of skin, is essential for understanding the complex processes of skin aging, wound healing and disease development. Further, the quantitative, automatic assessment of these changes enables to analyze large amounts of image data in an abstract but comprehensive manner. 
    However, previous work using OCT with methods of angiography was imaging less scattering, hence more challenging tissue than skin, such as brain and retina tissue. The published methods for capillary segmentation were mostly non-automatic, poorly benchmarked against state-of-the-art methods of computer vision and not applied to investigate medical processes and studies in a comprehensive fashion. 
    Here, segmentation of capillaries in skin is reported and its efficacy is demonstrated in both, a 
    longitudinal mouse study and a preliminary study in humans. By combining state-of-the-art image 
    processing methods in an optimized way, we were able to improve the segmentation results and analyze the impact of each post-processing step. 
    Furthermore, this automatic segmentation enabled us to analyze big amounts of 
    datasets automatically and derive meaningful conclusions for the planning of clinical studies. 
    With this work, optical coherence tomography is combined with methods of computer vision to a diagnostic 
    tool with unique capabilities to characterize vascular diversity and provide extraordinary 
    opportunities for dermatological investigation in both, clinics and research.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Hinnerk Schulz-Hildebrandt and Michael Münter and Martin Ahrens and Hendrik Spahr and Dierck Hillmann and Peter König and Gereon Hüttmann: Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations. in 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources, no. 10591, pp. 105910O, 2018
    BibTeX Link
    @inproceedings{Schulz-Hildebrandt2018a,
    title = {Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations},
    author = {Hinnerk Schulz-Hildebrandt and Michael M\"{u}nter and Martin Ahrens and Hendrik Spahr and Dierck Hillmann and Peter K\"{o}nig and Gereon H\"{u}ttmann},
    doi = {10.1117/12.2303755},
    isbn = {9781510616745},
    year = {2018},
    date = {2018-03-05},
    booktitle = {2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources},
    volume = {10591},
    pages = {105910O},
    abstract = {Optical coherence tomography (OCT) images scattering tissues with 5 to 15 μm resolution. This is usually not sufficient for a distinction of cellular and subcellular structures. Increasing axial and lateral resolution and compensation of artifacts caused by dispersion and aberrations is required to achieve cellular and subcellular resolution. This includes defocus which limit the usable depth of field at high lateral resolution. OCT gives access the phase of the scattered light and hence correction of dispersion and aberrations is possible by numerical algorithms. Here we present a unified dispersion/aberration correction which is based on a polynomial parameterization of the phase error and an optimization of the image quality using Shannon’s entropy. For validation, a supercontinuum light sources and a costume-made spectrometer with 400 nm bandwidth were combined with a high NA microscope objective in a setup for tissue and small animal imaging. Using this setup and computation corrections, volumetric imaging at 1.5 μm resolution is possible. Cellular and near cellular resolution is demonstrated in porcine cornea and the drosophila larva, when computational correction of dispersion and aberrations is used. Due to the excellent correction of the used microscope objective, defocus was the main contribution to the aberrations. In addition, higher aberrations caused by the sample itself were successfully corrected. Dispersion and aberrations are closely related artifacts in microscopic OCT imaging. Hence they can be corrected in the same way by optimization of the image quality. This way microscopic resolution is easily achieved in OCT imaging of static biological tissues.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Uzunbajakava, N. E. and Varghese, B. and Botchkareva, N. V. and Verhagen, R. and Vogel, A.: Highlighting the nuances behind interaction of picosecond pulses with human skin: Relating distinct laser-tissue interactions to their potential in cutaneous interventions. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE, no. 0492 ,, 2018
    BibTeX Link
    @inproceedings{Vogel2018,
       author = {Uzunbajakava, N. E. and Varghese, B. and Botchkareva, N. V. and Verhagen, R. and Vogel, A.},
       title = {Highlighting the nuances behind interaction of picosecond pulses with human skin: Relating distinct laser-tissue interactions to their potential in cutaneous interventions},
       booktitle = {Progress in Biomedical Optics and Imaging - Proceedings of SPIE},
       volume = {10492} ,
       DOI = {10.1117/12.2307804}
       year = {2018}
    date = {2018-20-02},
       type = {Conference Proceedings}
    }
  • Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Gereon Hüttmann and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten: Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation. Journal of Medical Robotics Research, no. 3, pp. 184005, 2018
    BibTeX Link Link
    @article{borghesan2018single,
    title = {Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation},
    author = {Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten},
    editor = {World Scientific},
    url = {https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    doi = {10.1142/S2424905X18400056},
    year = {2018},
    date = {2018-02-12},
    journal = {Journal of Medical Robotics Research},
    volume = {3},
    number = {02},
    pages = {184005},
    abstract = {Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures or to inadvertently injure the underlying retina. Damage to the latter is mostly irreparable and might cause permanent loss of vision. Despite the availability over excellent stereo microscopes, wide-angle lenses and powerful light source visualization remains a problem. Especially, the limited depth perception is still perceived as a major bottle-neck whereas efforts have been conducted to integrate sensing capability in today’s state-of-the-art instruments, so far, little effort has been paid to process the obtained sensor data and turns this into a reliable source of information upon which robot assistive guidance schemes could be endowed upon. This paper proposes a number of processing techniques tailored to Optical Coherence Tomography (OCT) measurements. The first results of the proposed algorithms show that it is feasible to extract good and reliable distance estimates from this otherwise rather noisy signal and from a fairly limited dataset. The used data are the so-called A-scans. These are OCT measurements consisting of a single-line image that could be captured by an instrument-mounted fiber through which the OCT signal passes back-and-forth. However, in this work, we perform a pilot study whereby the employed A-scans are extracted from B-scans that are captured by a microscope-mounted OCT scanner, rather than obtained from a probe. The performance of a first embodiment of the algorithm that is based on an Unscented Kalman Filter (UKF) is compared to the performance of a second embodiment that relies on a Particle Filter (PF), focusing on the issues in filter initialization and the tracking quality. Finally, results of UKF and PF executions on a validation dataset are presented. 
    Read More: https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Maertz, J. and Kolb, J. P. and Klein, T. and Mohler, K. J. and Eibl, M. and Wieser, W. and Huber, R. and Priglinger, S. and Wolf, A.: Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm. Graefe`s Archive for Clinical and Experimental Ophthalmology, no. 256, pp. 289-298, 2018
    BibTeX Link Link
    @article{Maertz2018,
       author = {Maertz, J. and Kolb, J. P. and Klein, T. and Mohler, K. J. and Eibl, M. and Wieser, W. and Huber, R. and Priglinger, S. and Wolf, A.},
       title = {Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
       volume = {256},
       number = {2},
       pages = {289-298},
       DOI = {10.1007/s00417-017-3857-9},
       url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032262413&doi=10.1007%2fs00417-017-3857-9&partnerID=40&md5=a46c315f12cf5e633ea0f7e644116eb3},
       year = {2018},
       Keywords= {En face imaging, Optical coherence tomography, Swept-source OCT, Megahertz OCT, 3D rendering, Optic disc, Optic disc pit, Optic disc pit maculopathy}
       type = {Journal Article}
    }
  • Hinnerk Schulz-Hildebrandt and Mario Pieper and Charlotte Stehmar and Martin Ahrens and Christian Idel and Barbara Wollenberg and Peter König and Gereon Hüttmann: Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography. Biomedical Optics Express, no. 9, pp. 636-647, 2018
    BibTeX Link Link
    @article{schulz2018novel,
    title = {Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography},
    author = {Hinnerk Schulz-Hildebrandt and Mario Pieper and Charlotte Stehmar and Martin Ahrens and Christian Idel and Barbara Wollenberg and Peter K\"{o}nig and Gereon H\"{u}ttmann},
    editor = {Optical Society of America},
    url = {https://www.osapublishing.org/abstract.cfm?URI=boe-9-2-636
    https://www.osapublishing.org/boe/viewmedia.cfm?uri=boe-9-2-636&seq=0},
    doi = {10.1364/BOE.9.000636},
    isbn = {10.1364/BOE.9.000636},
    year = {2018},
    date = {2018-01-16},
    journal = {Biomedical Optics Express},
    volume = {9},
    number = {2},
    pages = {636-647},
    abstract = {Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 \textendash 2 μm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests overall a better image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 μm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was determined successfully. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.},
    keywords = {Endoskope, OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Han, J. W. and Choi, J. / Kim, Y. S. and Kim, J. / Brinkmann, R. / Lyu, J. and Park, T. K.: Comparison of the neuroinflammatory responses to selective retina therapy and continuous-wave laser photocoagulation in mouse eyes. Graefe`s Archive for Clinical and Experimental Ophthalmology, no. 256, pp. 341-353, 2018
    BibTeX Link
    @article{Brinkmann,
       author = {Han, J. W. and Choi, J. / Kim, Y. S. and Kim, J. / Brinkmann, R. / Lyu, J. and Park, T. K.},
       title = {Comparison of the neuroinflammatory responses to selective retina therapy and continuous-wave laser photocoagulation in mouse eyes},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
       volume = {256},
       number = {2},
       pages = {341-353},
     doi=10.1007%2fs00417-017-3883-7&partnerID=40&md5=26bbcc9f8a2717104bde511ddda58a6d},
       year = {2018},
       type = {Journal Article}
    }
    
  • Kolb, Jan Philip and Pfeiffer, Tom and Eibl, Matthias and Hakert, Hubertus and Huber, Robert: High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates. Biomedical Optics Express, no. 9, pp. 120-130, 2018
    BibTeX Link
    @article{Kolb2018,
       author = {Kolb, Jan Philip and Pfeiffer, Tom and Eibl, Matthias and Hakert, Hubertus and Huber, Robert},
       title = {High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates},
       journal = {Biomedical Optics Express},
       volume = {9},
       number = {1},
       pages = {120-130},
       DOI = {10.1364/BOE.9.000120},
       year = {2018},
       type = {Journal Article}
    }
    

2017

  • Jens Horstmann and Hinnerk Schulz-Hildebrandt and Felix Bock and Sebastian Siebelmann and Eva Lankenau and Gereon Hüttmann and Philipp Steven and Claus Cursiefen: Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography. Investigative Ophthalmology & Visual Science, no. 58, pp. 5880-5886, 2017
    BibTeX Link Link
    @article{Horstmann2017,
    title = {Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography},
    author = {Jens Horstmann and Hinnerk Schulz-Hildebrandt and Felix Bock and Sebastian Siebelmann and Eva Lankenau and Gereon H\"{u}ttmann and Philipp Steven and Claus Cursiefen},
    editor = {The Association Research for in Vision and Ophthalmology},
    url = {http://iovs.arvojournals.org/article.aspx?articleid=2664271},
    doi = {10.1167/iovs.17-22286},
    isbn = {1552-5783},
    year = {2017},
    date = {2017-12-25},
    journal = {Investigative Ophthalmology & Visual Science},
    volume = {58},
    number = {13},
    pages = {5880-5886},
    abstract = {Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). 
    
    Methods: Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were acquired using a custom-built mOCT system enabling a resolution of 1 μm at a B-scan rate of 165/s. Three of the six animals received an additional intrastromal injection of India ink 24 hours before the measurement to stain the corneal lymphatic system in vivo. Immunohistochemistry using CD31 and LYVE-1 was used to validate the mOCT findings. 
    
    Results: Using mOCT, lymphatic vessels were visible as dark vessel-like structures with the lumen lacking a hyperreflective wall and mostly lacking cells. However, individual, slowly moving particles, which most likely are immune cells, occasionally could be observed inside the lumen. In lymphatic vessels of ink-stained corneas, hyperreflection and shadowing underneath was observed. Ink-filled lymphatic vessels were colocalized in consecutive corneal flat mounts of the same specimen. 
    
    Conclusions: Corneal lymphatic vessels can be imaged using mOCT. This novel approach opens new options for noninvasive clinical imaging of corneal lymphatic vessels for diagnostic and therapeutic indications.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Seiler, T. G. and Engler, M. and Beck, E. and Birngruber, R. and Kochevar, I. E.: Interface bonding with corneal crosslinking (CXL) after LASIK ex vivo. Investigative Ophthalmology and Visual Science, no. 58, pp. 6292-6298, 2017
    BibTeX Link
    @article{Seiler2017,
       author = {Seiler, T. G. and Engler, M. and Beck, E. and Birngruber, R. and Kochevar, I. E.},
       title = {Interface bonding with corneal crosslinking (CXL) after LASIK ex vivo},
       journal = {Investigative Ophthalmology and Visual Science},
       volume = {58},
       number = {14},
       pages = {6292-6298},
       DOI = {10.1167/iovs.17-22426},
       
       year = {2017},
       type = {Journal Article}
    }
    
  • Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.: Noncontact holographic detection for photoacoustic tomography. J Biomed Opt, no. 22, pp. 1-14, 2017
    BibTeX Link
    @article{Buj2017,
       author = {Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.},
       title = {Noncontact holographic detection for photoacoustic tomography},
       journal = {J Biomed Opt},
       volume = {22},
       number = {10},
       pages = {1-14},
       DOI = {10.1117/1.jbo.22.10.106007},
       year = {2017},
       type = {Journal Article}
    }
    
    
    
  • Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.: Power-controlled temperature guided retinal laser therapy. J Biomed Opt, no. 22, pp. 1-11, 2017
    BibTeX Link
    @article{Baade2017,
       author = {Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Power-controlled temperature guided retinal laser therapy},
       journal = {J Biomed Opt},
       volume = {22},
       number = {11},
       pages = {1-11},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.22.11.118001},
       year = {2017},
       type = {Journal Article}
    }
    
  • Matthias Eibl and Sebastian Karpf and Hubertus Hakert and Torben Blömker and Jan Philip Kolb and Christian Jirauschek and Robert Huber: Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification. Opt. Lett., no. 42, pp. 4406-4409, OSA, Nov, 2017
    BibTeX Link
    @article{2017_HU_Eibl_b_OL,
    author = {Matthias Eibl and Sebastian Karpf and Hubertus Hakert and Torben Bl\"{o}mker and Jan Philip Kolb and Christian Jirauschek and Robert Huber},
    journal = {Opt. Lett.},
    keywords = {NLI, TPEF, Multiphoton},
    number = {21},
    pages = {4406--4409},
    publisher = {OSA},
    title = {Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification},
    volume = {42},
    month = {Nov},
    year = {2017},
    doi = {10.1364/OL.42.004406},
    }
    
  • Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.: Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea. Am J Pathol, no. 187, pp. 2451-2460, 2017
    BibTeX Link
    @article{Kretschmer2017,
       author = {Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.},
       title = {Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea},
       journal = {Am J Pathol},
       ISSN = {0002-9440},
       DOI = {10.1016/j.ajpath.2017.07.006},
      volume = {187},
      number = {11},
       year = {2017},
    pages = {2451-2460},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon: Off-axis reference beam for full-field swept-source OCT and holoscopy. Optics Express, no. 25, pp. 27770-27784, 2017
    BibTeX Link
    @article{Hillmann2017,
       author = {Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon},
       title = {Off-axis reference beam for full-field swept-source OCT and holoscopy},
       journal = {Optics Express},
       volume = {25},
       number = {22},
       pages = {27770-27784},
       DOI = {10.1364/OE.25.027770},
       year = {2017},
       type = {Journal Article}
    }
    
  • Bliedtner, Katharina and Seifert, Eric and Brinkmann, Ralf E. D. Amelink A.: Real Time Speckle Monitoring to Control Retinal Photocoagulation. in Proc. SPIE, no. 10413, pp. 1041308-1-7,
    BibTeX Link
    @inproceedings{Bliedtner2017,
       author = {Bliedtner, Katharina and Seifert, Eric and Brinkmann, Ralf E. D. Amelink A.},
       title = {Real Time Speckle Monitoring to Control Retinal Photocoagulation},
       booktitle = {Proc. SPIE},
       volume = {10413},
       pages = {1041308-1-7},
       url = {http://www.osapublishing.org/abstract.cfm?URI=ECBO-2017-1041308}
    }
    
  • Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.: ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch`s Membrane and Causes Its Structural Remodeling. Curr Eye Res, no. 3, pp. 1-12, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.},
       title = {ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch's Membrane and Causes Its Structural Remodeling},
       journal = {Curr Eye Res},
       pages = {1-12},
       ISSN = {0271-3683},
       DOI = {10.1080/02713683.2017.1370118},
       year = {2017},
       type = {Journal Article},
    volume = {3}
    }
    
  • Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.: Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects. J Vis Exp, no. 30, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.},
       title = {Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects},
       journal = {J Vis Exp},
       number = {124},
       ISSN = {1940-087x},
       DOI = {10.3791/54326},
       year = {2017},
       type = {Journal Article},
    volume = {30}
    
  • Sarah Latus and Christoph Otte and Matthias Schlüter and Josua Rehra and Kevin Bizon and Hinnerk Schulz-Hildebrandt and Thore Saathoff and Gereon Hüttmann and Alexander Schlaefer: An Approach for Needle Based Optical Coherence Elastography Measurements. in Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017, no. 10434, pp. 655-663, 2017
    BibTeX Link Link
    @conference{Latus2017,
    title = {An Approach for Needle Based Optical Coherence Elastography Measurements},
    author = {Sarah Latus and Christoph Otte and Matthias Schl\"{u}ter and Josua Rehra and Kevin Bizon and Hinnerk Schulz-Hildebrandt and Thore Saathoff and Gereon H\"{u}ttmann and Alexander Schlaefer},
    editor = {Springer International Publishing},
    url = {https://link.springer.com/chapter/10.1007/978-3-319-66185-8_74},
    doi = {https://doi.org/10.1007/978-3-319-66185-8_74},
    isbn = {978-3-319-66185-8},
    year = {2017},
    date = {2017-09-01},
    booktitle = {Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017},
    volume = {10434},
    pages = {655--663},
    organization = {Springer},
    abstract = {© Springer International Publishing AG 2017. 
    While navigation and interventional guidance are typically based on image data, the images do not necessarily reflect mechanical tissue properties. Optical coherence elastography (OCE) presents a modality with high sensitivity and very high spatial and temporal resolution. However, OCE has a limited field of view of only 2\textendash5 mm depth. We present a side-facing needle probe to image externally induced shear waves from within soft tissue. A first method of quantitative needle-based OCE is provided. Using a time of flight setup, we establish the shear wave velocity and estimate the tissue elasticity. For comparison, an external scan head is used for imaging. Results for four different phantoms indicate a good agreement between the shear wave velocities estimated from the needle probe at different depths and the scan head. The velocities ranging from 0.9\textendash3.4 m/s agree with the expected values, illustrating that tissue elasticity estimates from within needle probes are feasible.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Pfeiffer, Tom and Draxinger, Wolfgang and Grill, C and Huber, R: Long-range live 3D-OCT at different spectral zoom levels. in Proc. SPIE, no. 10416, pp. 104160L1-4, 2017
    BibTeX
    @inproceedings{HU_Pfeiffer2017,
       author = {Pfeiffer, Tom and Draxinger, Wolfgang and Grill, C and Huber, R},
       title = {Long-range live 3D-OCT at different spectral zoom levels},
       booktitle = {Proc. SPIE},
       volume = {10416},
       pages = {104160L1-4},
       year = {2017},
       type = {Conference Proceedings}
    }
    
  • Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation. International Journal of Nanomedicine, no. Volume 12, pp. 5659-5672, 2017
    BibTeX Link
    @article{Yao2017,
       author = {Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
    journal = {International Journal of Nanomedicine},
       volume = {Volume 12},
       pages = {5659-5672},
       DOI = {10.2147/IJN.S140620},
       year = {2017},
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Amenta, Gaetano and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and Soest, Gijs van: Thermo-elastic optical coherence tomography. Optics Letters, no. 42, pp. 3466-3469, 2017
    BibTeX Link
    @article{HU_Wang2017,
       author = {Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Amenta, Gaetano and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and Soest, Gijs van},
       title = {Thermo-elastic optical coherence tomography},
       journal = {Optics Letters},
       volume = {42},
       number = {17},
       pages = {3466-3469},
       DOI = {10.1364/OL.42.003466},
       year = {2017},
       type = {Journal Article}
    }
    
  • Kolb, Jan Philip and Klee, Julian and Pfeiffer, Tom and Huber, Robert: 1060nm FDML laser with centimeter coherence length and 1.67 MHz sweep rate for full eye length and retinal ultra-widefield OCT. in Proc. SPIE, no. 10416, pp. 104160J-104160J-6, 2017
    BibTeX Link
    @inproceedings{Kolb2017,
       author = {Kolb, Jan Philip and Klee, Julian and Pfeiffer, Tom and Huber, Robert},
       title = {1060nm FDML laser with centimeter coherence length and 1.67 MHz sweep rate for full     eye length and retinal ultra-widefield OCT},
      booktitle = {Proc. SPIE},
       volume = {10416},
      pages = {104160J-104160J-6},
      year = {2017},
       url = {http://dx.doi.org/10.1117/12.2286854},
       type = {Conference Proceedings}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Pfeiffer, Tom and Kolb, Jan Philip and Huber, Robert: Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup. in Proc. SPIE, no. 10414, pp. 1041403-1041403-7, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Pfeiffer, Tom and Kolb, Jan Philip and Huber, Robert},
       title = {Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup},
      booktitle = {Proc. SPIE},
       volume = {10414},
      year = {2017},
       pages = {1041403-1041403-7},
       url = {http://dx.doi.org/10.1117/12.2286035},
       type = {Conference Proceedings}
    }
    
  • Hakert, Hubertus and Eibl, Matthias and Karpf, Sebastian and Huber, Robert: Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition. in Proc. SPIE, no. 10414, pp. 1041408-1041408-7, 2017
    BibTeX Link
    @inproceedings{Hakert2017,
       author = {Hakert, Hubertus and Eibl, Matthias and Karpf, Sebastian and Huber, Robert},
       title = {Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition},
    booktitle = {Proc. SPIE},
    Year={2017},
       volume = {10414},
       pages = {1041408-1041408-7},
       url = {http://dx.doi.org/10.1117/12.2287947},
       type = {Conference Proceedings}
    }
    
  • Lange, Birgit and Jocham, Dieter and Brinkmann, Ralf and Cordes, Jens: Stone/tissue differentiation for Holmium laser lithotripsy using autofluorescence: Clinical proof of concept study. Lasers in Surgery and Medicine, no. 49, pp. 361-365, 2017
    BibTeX Link
    @article{Lange2017,
       author = {Lange, Birgit and Jocham, Dieter and Brinkmann, Ralf and Cordes, Jens},
       title = {Stone/tissue differentiation for Holmium laser lithotripsy using autofluorescence: Clinical proof of concept study},
       journal = {Lasers in Surgery and Medicine},
       volume = {49},
       number = {4},
       pages = {361-365},
       ISSN = {1096-9101},
       DOI = {10.1002/lsm.22611},
       year = {2017},
       type = {Journal Article}
    }
    
  • Maertz, J. and Mohler, K. J. and Kolb, J. P. and Kein, T. and Neubauer, A. and Kampik, A. and Priglinger, S. and Wieser, W. and Huber, R. and Wolf, A.: INTRAPAPILLARY PROLIFERATION IN OPTIC DISK PITS: Clinical Findings and Time-Related Changes. Retina, no. 37, pp. 906-914, 2017
    BibTeX Link
    @article{Maertz2017,
       author = {Maertz, J. and Mohler, K. J. and Kolb, J. P. and Kein, T. and Neubauer, A. and Kampik, A. and Priglinger, S. and Wieser, W. and Huber, R. and Wolf, A.},
       title = {INTRAPAPILLARY PROLIFERATION IN OPTIC DISK PITS: Clinical Findings and Time-Related Changes},
       journal = {Retina},
       volume = {37},
       number = {5},
       pages = {906-914},
       DOI = {10.1097/iae.0000000000001260},
       year = {2017},
       type = {Journal Article}
    }
    
  • Jirauschek, Christian and Huber, Robert: Efficient simulation of the swept-waveform polarization dynamics in fiber spools and Fourier domain mode-locked (FDML) lasers. Journal of the Optical Society of America B, no. 34, pp. 1135-1146, 2017
    BibTeX Link
      @article{Jirauschek2017,
       author = {Jirauschek, Christian and Huber, Robert},
       title = {Efficient simulation of the swept-waveform polarization dynamics in fiber spools and Fourier domain mode-locked (FDML) lasers},
       journal = {Journal of the Optical Society of America B},
       volume = {34},
       number = {6},
       pages = {1135-1146},
       abstract = {We present a theoretical model and its efficient numerical implementation for the simulation of wavelength-swept waveform propagation in fiber systems such as Fourier domain mode-locked (FDML) lasers, fully accounting for the polarization dynamics in fiber spools and further polarization-dependent optical components in the setup. This approach enables us to perform long-time simulations of the FDML laser dynamics over more than 100,000 cavity round trips, as required for some FDML configurations to ensure convergence to the steady-state operating regime. The model is validated against experimental results for single propagation through a fiber spool and for stationary FDML operation. The polarization dynamics due to the fiber spool, inducing polarization-mode dispersion, bending birefringence as well as cross-phase modulation, and other optical components such as the Faraday-rotating mirror used for polarization compensation is thoroughly investigated.},
       keywords = {Laser theory
    Lasers, tunable
    Optical coherence tomography
    Birefringence
    Polarization
    Pulses},
       url = {https://www.osapublishing.org/josab/abstract.cfm?uri=josab-34-6-1135},
       year = {2017},
       type = {Journal Article}
    }
    
  • Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Richard Neffin and Peter Koch and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten: Probabilistic Principal Component Analysis and Particle Filtering for real-time retina detection from a single-fiber OCT. in Proceedings of the 7th Joint Workshop on New Technologies for Computer/Robot Assisted Surgery, 2017
    BibTeX
    @conference{Borghesan2017,
    title = {Probabilistic Principal Component Analysis and Particle Filtering for real-time retina detection from a single-fiber OCT},
    author = {Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Richard Neffin and Peter Koch and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten},
    year = {2017},
    date = {2017-06-02},
    booktitle = {Proceedings of the 7th Joint Workshop on New Technologies for Computer/Robot Assisted Surgery},
    abstract = {Vitreo-retinal surgery concerns a set of particularly demanding micro-surgical interventions that take place at the back of the eye. Examples of such procedures are retinal vein cannulation (where the surgeon aims to insert a needle in a vein of the size of human hairs) and epiretinal membrane peeling (where a detached membrane must be removed from the retina). As severe retinal damage can be caused by undesired collisions, good instrument to retina distance perception would be very useful. We propose to use an OCT-fiber instrumented tool, and purposefully designed algorithms to interpret the measurements and extract a reliable real-time distance estimate. This abstract describes the progress that was made and includes a test conducted with a robotic platform on a synthetic eye mockup.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber: Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate. Biomedical Optics Express, no. 8, pp. 3132-3142, OSA, Jul, 2017
    BibTeX Link
    @article{HU_2017_Eibla_BOE,
    author = {Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber},
    journal = {Biomedical Optics Express},
    number = {7},
    pages = {3132--3142},
    publisher = {OSA},
    title = {Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate},
    volume = {8},
    month = {Jul},
    year = {2017},
    doi = {10.1364/BOE.8.003132}
    }
    
  • Verbytskyi, Ievgen and Münter, Michael and Buj, Christian and Brinkmann, Ralf: A Problem of a Displacement Calculation of Tissue Surface in Non-Contact Photoacoustic Tomography. Naukovi Visti NTUU KPI, pp. 58-64, 2017
    BibTeX Link
    @article{Verbytskyi2017,
       author = {Verbytskyi, Ievgen and Münter, Michael and Buj, Christian and Brinkmann, Ralf},
       title = {A Problem of a Displacement Calculation of Tissue Surface in Non-Contact Photoacoustic Tomography},
       journal = {Naukovi Visti NTUU KPI},
       number = {2},
       pages = {58-64},
       ISSN = {2519-8890},
       url = {http://dx.doi.org/10.20535/1810-0546.2017.2.98021},
       year = {2017},
       type = {Journal Article}
    }
    
  • Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon Hüttmann and Dieter Manstein: Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation). in Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery, no. 10037OS, 2017
    BibTeX Link
    @conference{Casper2017,
    title = {Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)},
    author = {Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon H\"{u}ttmann and Dieter Manstein},
    editor = {Spie},
    doi = {doi: 10.1117/12.2251485},
    year = {2017},
    date = {2017-04-19},
    booktitle = {Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery},
    volume = {10037OS},
    abstract = {In dermatology the reflexes of vasoconstriction and vasodilation are known as important mechanisms of thermoregulation of the inner body. Imaging the physiology of microvasculature of the skin with high spatial resolution in three dimensions while reacting to changes in temperature is crucial for understanding the complex processes of vasodynamics, which result in constriction and dilation of vessels. However, previous studies using Laser-Doppler flowmetry and -imaging could not provide reliable angiographic images which allow to quantify changes in blood vessel diameter. Here, we report a different approach for angiographic imaging of microvasculature of a anaesthetized rodent model using speckle variance optical coherence tomography (svOCT) during and after localized cooling. Therefore a commercial OCT with a center wavelength of 1.3 μm and a spatial resolution of 13µm was used in combination with a custom built cooling device to image such reflexes at the mouse ear pinna and dorsal skinfold. Cooling was applied in steps of 2−5◦ C starting at the baseline temperature of 27◦ C down to −10◦ C. To our surprise and in contrast to the general opinion in literature, we were able to observe that the majority of vessels with a diameter larger than 20 μm maintain perfused with a constant diameter when the tissue is cooled from baseline to subzero temperatures. However, vasoconstriction was observed very rarely and only in veins, which led to their occlusion. The results of this experiment lead us to reconsider essential aspects of previous understanding of temperature-induced vasodynamics in cutaneous microvasculature.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Kepp, Timo and Koinzer, Stefan and Handels, Heinz and Brinkmann, Ralf: Registrierung von nicht sichtbaren Laserbehandlungsarealen der Retina in Live-Aufnahmen des Fundus. in Bildverarbeitung für die Medizin 2017: Algorithmen - Systeme - Anwendungen. Proceedings des Workshops vom 12. bis 14. März 2017 in Heidelberg, pp. 331-336, Springer Berlin Heidelberg, Berlin, Heidelberg, 2017
    BibTeX Link
    @inbook{Kepp2017,
       author = {Kepp, Timo and Koinzer, Stefan and Handels, Heinz and Brinkmann, Ralf},
       title = {Registrierung von nicht sichtbaren Laserbehandlungsarealen der Retina in Live-Aufnahmen des Fundus},
       booktitle = {Bildverarbeitung für die Medizin 2017: Algorithmen - Systeme - Anwendungen. Proceedings des Workshops vom 12. bis 14. März 2017 in Heidelberg},
       editor = {Maier-Hein, geb Fritzsche Klaus Hermann and Deserno, geb Lehmann Thomas Martin and Handels, Heinz and Tolxdorff, Thomas},
       publisher = {Springer Berlin Heidelberg},
       address = {Berlin, Heidelberg},
       pages = {331-336},
       ISBN = {978-3-662-54345-0},
       url = {http://dx.doi.org/10.1007/978-3-662-54345-0_74},
       year = {2017},
       type = {Book Section}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Shot-Noise Limited Time-Encoded Raman Spectroscopy. Journal of Spectroscopy, no. 2017, pp. 1-6, 2017
    BibTeX Link
    @article{Karpf2017,
       author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
       title = {Shot-Noise Limited Time-Encoded Raman Spectroscopy},
       journal = {Journal of Spectroscopy},
       volume = {2017},
       pages = {1-6},
       url = {https://doi.org/10.1155/2017/9253475},
       year = {2017},
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs: Short pulse laser induced thermo-elastic deformation imaging. in Proc. SPIE, no. 10062, pp. 100620C-100620C-4, 2017
    BibTeX Link
    @inproceedings{Wang2017,
       author = {Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs},
       title = {Short pulse laser induced thermo-elastic deformation imaging},
    booktitle = Proc. SPIE},
      volume = {10062},
      year = {2017},
       pages = {100620C-100620C-4},
      url = {http://dx.doi.org/10.1117/12.2251502},
       type = {Conference Proceedings}
    }
    
    
  • Laves, Max-Heinrich and Schoob, Andreas and Kahrs, Lüder A. and Pfeiffer, Tom and Huber, Robert and Ortmaier, Tobias: Feature tracking for automated volume of interest stabilization on 4D-OCT images. in Proc. SPIE, no. 10135, pp. 101350W-101350W-7, 2017
    BibTeX Link
    @inproceedings{Laves2017,
       author = {Laves, Max-Heinrich and Schoob, Andreas and Kahrs, Lüder A. and Pfeiffer, Tom and Huber, Robert and Ortmaier, Tobias},
       title = {Feature tracking for automated volume of interest stabilization on 4D-OCT images},
     booktitle = {Proc. SPIE},
       volume = {10135},
       pages = {101350W-101350W-7},
      year = {2017},
       url = {http://dx.doi.org/10.1117/12.2255090},
       type = {Conference Proceedings}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert: Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging. in Proc. SPIE, no. 10083, pp. 100831C-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert},
       title = {Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging},
    booktitle = {Proc. SPIE },
       volume = {10083},
       pages = {100831C-6},
       year= {2017},
       doi= {10.1117/12.2251965},
       type = {Conference Proceedings}
    }
    
  • Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon: Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]. Biomedical Optics Express, no. 8, pp. 1499-1511, 2017
    BibTeX Link
    @article{Pfäffle2017,
       author = {Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon},
       title = {Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {3},
       pages = {1499-1511},
       keywords = {Image reconstruction-restoration
    Optical coherence tomography},
       url = {http://www.osapublishing.org/boe/abstract.cfm?URI=boe-8-3-1499},
       year = {2017},
       type = {Journal Article}
    }
    
  • Pfeiffer, Tom and Draxinger, Wolfgang and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Huber, Robert: Analysis of FDML lasers with meter range coherence. in Proc. SPIE, no. 10053, pp. 100531T-100531T-6, 2017
    BibTeX
    @inproceedings{Pfeiffer2017,
       author = {Pfeiffer, Tom and Draxinger, Wolfgang and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Huber, Robert},
       title = {Analysis of FDML lasers with meter range coherence},
       booktitle = {Proc. SPIE},
       volume = {10053},
       pages = {100531T-100531T-6},
      year = {2017},
       type = {Conference Proceedings}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert: Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection. in Proc. SPIE, no. 10069, pp. 100691F-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert},
       title = {Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection},
    booktitle = {Proc. SPIE },
       volume = {10069},
       pages = {100691F-6},
       doi= {10.1117/12.2250831},
      year={2017},
       type = {Conference Proceedings}
    }
    
  • Klein, Thomas and Huber, Robert: High-speed OCT light sources and systems [Invited]. Biomedical Optics Express, no. 8, pp. 828-859, 2017
    BibTeX Link
    @article{Klein2017,
       author = {Klein, Thomas and Huber, Robert},
       title = {High-speed OCT light sources and systems [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {2},
       pages = {828-859},
       abstract = {Imaging speed is one of the most important parameters that define the performance of optical coherence tomography (OCT) systems. During the last two decades, OCT speed has increased by over three orders of magnitude. New developments in wavelength-swept lasers have repeatedly been crucial for this development. In this review, we discuss the historical evolution and current state of the art of high-speed OCT systems, with focus on wavelength swept light sources and swept source OCT systems.},
       keywords = {Imaging systems
    Optical coherence tomography
    Lasers and laser optics
    Lasers, tunable},
       DOI = {10.1364/BOE.8.000828},
       year = {2017},
       type = {Journal Article}
    }
    

2016

  • Tromberg, B. J. and Anderson, R. R. and Birngruber, R. and Brinkmann, R. and Berns, M. W. and Parrish, J. A. and Apiou-Sbirlea, G.: Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health. J Biomed Opt, no. 21, pp. 124001, 2016
    BibTeX Link
    @article{RN5034,
       author = {Tromberg, B. J. and Anderson, R. R. and Birngruber, R. and Brinkmann, R. and Berns, M. W. and Parrish, J. A. and Apiou-Sbirlea, G.},
       title = {Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health},
       journal = {J Biomed Opt},
       volume = {21},
       number = {12},
       pages = {124001},
       ISSN = {1560-2281 (Electronic)
    1083-3668 (Linking)},
       DOI = {10.1117/1.JBO.21.12.124001},   
       year = {2016},
       type = {Journal Article}
    }
    
  • Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon: In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography. Optics Letters, no. 41, pp. 4987-4990, 2016
    BibTeX Link
    @article{Sudkamp2016,
       author = {Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon},
       title = {In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography},
       journal = {Optics Letters},
       volume = {41},
       number = {21},
       pages = {4987-4990},
       abstract = {With a simple setup, mainly composed of a low coherence light source and a camera, full-field optical coherence tomography (FF-OCT) allows volumetric tissue imaging. However, fringe washout constrains its use in retinal imaging. Here, we present a novel motion-insensitive approach to FF-OCT, which introduces path-length differences between the reference and the sample light in neighboring pixels using an off-axis reference beam. The temporal carrier frequency in scanned time-domain OCT is replaced by a spatial carrier frequency. Volumetric in-vivo FF-OCT measurements of the human retina were acquired in only 1.3 s, comparable to the acquisition times of current clinically used OCT devices.},
       keywords = {Imaging systems
    Coherence imaging
    Optical coherence tomography
    Frequency filtering
    Interferometric imaging},
    DOI = {10.1364/OL.41.004987}
     url= {https://www.osapublishing.org/ol/abstract.cfm?uri=ol-41-21-4987}  
       year = {2016},
       type = {Journal Article}
    }
    
  • Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.: Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT. Klin Monatsbl Augenheilkd, no. 233, pp. 1324-1330, 2016
    BibTeX Link
    @article{Spahr2016,
       author = {Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.},
       title = {Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT},
       journal = {Klin Monatsbl Augenheilkd},
       volume = {233},
       number = {12},
       pages = {1324-1330},
       ISSN = {0023-2165},
       DOI = {10.1055/s-0042-120279},
       year = {2016},
       type = {Journal Article}
    }
    
  • Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.: Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. 1-48, 2016
    BibTeX Link
    @article{Schulz-Hildebrandt2016,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.},
       title = {Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {1-48},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584651},
       year = {2016},
       type = {Journal Article}
    }
    
  • Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf: Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results. Investigative Ophthalmology & Visual Science, no. 57, pp. 5852-5852, 2016
    BibTeX Link
    @article{Hüttmann2016,
       author = {Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf},
       title = {Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {57},
       number = {12},
       pages = {5852-5852},
       abstract = {Abstract Purpose : The molecular processes during heating with a photocoagulation laser, particularly in sub-visible or mere thermal stimulation treatment, have only partly been understood, and different theories exist that try to explain its clinical efficacy. Optical coherence tomography (OCT) was successfully used to grade lesions with high accuracy 1 hour after the treatments and beyond. During the irradiation, changes in tissue scattering and, by use of the Doppler signal, tissue motion caused by thermal expansion and coagulation-induced tissue contraction were shown to correlate ex-vivo and in rabbits with the strength of photocoagulation lesions. Aim of this study was to validate feasibility and reproducibility of these results in humans. Methods : In an ongoing study more than 100 lesions of three patients have been imaged with a slitlamp-based OCT (1060 nm, 90,000 A-scans/s) with varying irradiance during laser exposure. Durations of the exposure were 50 ms and 200 ms; spot size was 300 µm. Eye movements and heart beat were corrected by cross-correlation of the images. Increased tissue scattering and movement of the neuronal retina due to thermal expansion were determined from the image sequences with 3 ms temporal resolution. Results : In the first treatments with this prototype device, we received acceptable image quality in 1/3 of the lesions. Changes in the neuronal retina were successful visualized during and after the laser irradiation, demonstrating the feasibility of a real-time assessment of initial effects of photocoagulation in humans. Lesion visibility in standard, reflection-based OCT was much weaker during treatment compared to 1 hour afterwards. Increased tissue scattering was observed in stronger lesions already during the laser irradiation. At reduced irradiance, scattering increase was only observed after the end of irradiation. However, tissue motion towards the vitreous was still observed in these cases. Conclusions : In conclusion, high-speed OCT recording during photocoagulation measures initial tissue changes during photocoagulation in humans. It may enhance our understanding of the tissue dynamics right after laser irradiation. It may provide useful information for a real-time dosage control as well. This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2016},
       type = {Journal Article}
    }
    
  • Kim, H. D. and Jang, S. Y. and Lee, S. H. and Kim, Y. S. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.: Retinal Pigment Epithelium Responses to Selective Retina Therapy in Mouse Eyes. Invest Ophthalmol Vis Sci, no. 57, pp. 3486-95, 2016
    BibTeX Link
    @article{Kim2016,
       author = {Kim, H. D. and Jang, S. Y. and Lee, S. H. and Kim, Y. S. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.},
       title = {Retinal Pigment Epithelium Responses to Selective Retina Therapy in Mouse Eyes},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {57},
       number = {7},
       pages = {3486-95},
       abstract = {PURPOSE: To investigate the characteristics of retinal pigment epithelium (RPE) and retinal damage induced by selective retina therapy (SRT) in mice, and to elucidate longitudinal changes in RPE cells. METHODS: C57BL/6J mice received SRT and continuous-wave laser photocoagulation (cwPC). The cell death pattern was evaluated using TUNEL assay, and proliferative potential of the RPE cells was evaluated using 5-ethynyl-2'-dexoyuridine (EdU) assay. To investigate the cell-cell integrity of RPE cells, beta-catenin staining was performed. The number and hexagonality of RPE cells in the SRT-treated area were estimated using a Voronoi diagram with time periods of 3 hours to 14 days. Antibodies to microphthalmia-associated transcription factor (MiTF) and orthodenticle homeobox 2 (Otx2) were used to confirm the specific characteristics of RPE cells in the SRT-treated area. RESULTS: The number of TUNEL-positive cells located in the neural retina was significantly lower in lesions treated with SRT compared to those treated with cwPC. EdU-positive RPE cells were first detected 3 to 12 hours after SRT, and increased until 3 to 7 days after SRT. beta-catenin staining showed that hexagonality was compromised and subsequently, RPE cells expanded in size within the targeted location. The number of RPE cells in SRT lesions decreased gradually until 12 hours after SRT and recovered by 14 days. Upregulated expression of MiTF and Otx2 was observed for 2 weeks in the SRT lesions. CONCLUSIONS: Selective retina therapy seems to induce selective RPE damage without collateral thermal injury in the neural retina. Furthermore, SRT-treated lesions recovered by proliferation of RPE cells that were present in the treated lesions and by expansion of adjacent RPE cells.},
       ISSN = {0146-0404},
       DOI = {10.1167/iovs.16-19508},
       year = {2016},
       type = {Journal Article}
    }
    
  • Bliedtner, Katharina and Seifert, Eric and Stockmann, Leoni and Effe, Lisa and Brinkmann, Ralf: Towards real time speckle controlled retinal photocoagulation. no. 9693, pp. 96931A-96931A-6,
    BibTeX Link
    @inproceedings{Bliedtner2016,
       author = {Bliedtner, Katharina and Seifert, Eric and Stockmann, Leoni and Effe, Lisa and Brinkmann, Ralf},
       title = {Towards real time speckle controlled retinal photocoagulation},
       volume = {9693},
       pages = {96931A-96931A-6},
       note = {10.1117/12.2212703},
       abstract = {Photocoagulation is a laser treatment widely used for the therapy of several retinal diseases. Intra- and inter-individual variations of the ocular transmission, light scattering and the retinal absorption makes it impossible to achieve a uniform effective exposure and hence a uniform damage throughout the therapy. A real-time monitoring and control of the induced damage is highly requested. Here, an approach to realize a real time optical feedback using dynamic speckle analysis is presented. A 532 nm continuous wave Nd:YAG laser is used for coagulation. During coagulation, speckle dynamics are monitored by a coherent object illumination using a 633nm HeNe laser and analyzed by a CMOS camera with a frame rate up to 1 kHz. It is obvious that a control system needs to determine whether the desired damage is achieved to shut down the system in a fraction of the exposure time. Here we use a fast and simple adaption of the generalized difference algorithm to analyze the speckle movements. This algorithm runs on a FPGA and is able to calculate a feedback value which is correlated to the thermal and coagulation induced tissue motion and thus the achieved damage. For different spot sizes (50-200 μm) and different exposure times (50-500 ms) the algorithm shows the ability to discriminate between different categories of retinal pigment epithelial damage ex-vivo in enucleated porcine eyes. Furthermore in-vivo experiments in rabbits show the ability of the system to determine tissue changes in living tissue during coagulation.},
       url = {http://dx.doi.org/10.1117/12.2212703},
       type = {Conference Proceedings}
    }
    
  • Schlott, Kerstin and Koinzer, Stefan and Baade, Alexander and Birngruber, Reginald and Roider, Johann and Brinkmann, Ralf: Lesion strength control by automatic temperature guided retinal photocoagulation. Journal of Biomedical Optics, no. 21, pp. 098001-098001, 2016
    BibTeX Link
    @article{Schlott2016,
       author = {Schlott, Kerstin and Koinzer, Stefan and Baade, Alexander and Birngruber, Reginald and Roider, Johann and Brinkmann, Ralf},
       title = {Lesion strength control by automatic temperature guided retinal photocoagulation},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {9},
       pages = {098001-098001},
       note = {10.1117/1.JBO.21.9.098001},
       abstract = {Abstract.  Laser photocoagulation is an established treatment for a variety of retinal diseases. However, when using the same irradiation parameter, the size and strength of the lesions are unpredictable due to unknown inter- and intraindividual optical properties of the fundus layers. The aim of this work is to investigate a feedback system to generate desired lesions of preselectable strengths by automatically controlling the irradiation time. Optoacoustics were used for retinal temperature monitoring. A 532-nm continuous wave Nd:YAG laser was used for photocoagulation. A 75-ns/523-nm Q-switched Nd:YLF laser simultaneously excited temperature-dependent pressure transients, which were detected at the cornea by an ultrasonic transducer embedded in a contact lens. The temperature data were analyzed during the irradiation by a LabVIEW routine. The treatment laser was switched off automatically when the required lesion strength was achieved. Five different feedback control algorithms for different lesion sizes were developed and tested on rabbits in vivo. With a laser spot diameter of 133  μm, five different lesion types with ophthalmoscopically visible diameters ranging mostly between 100 and 200  μm, and different appearances were achieved by automatic exposure time control. The automatically controlled lesions were widely independent of the treatment laser power and the retinal pigmentation.},
       ISSN = {1083-3668},
       DOI = {10.1117/1.JBO.21.9.098001},
       year = {2016},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: In vivo optical imaging of physiological responses to photostimulation in human photoreceptors. PNAS Early Edition, pp. 1-6, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {In vivo optical imaging of physiological responses to photostimulation in human photoreceptors},
       journal = {PNAS Early Edition},
       pages = {1-6},
       abstract = {Noninvasive functional imaging of molecular and cellular processes of vision may have immense impact on research and clinical diagnostics. Although suitable intrinsic optical signals (IOSs) have been observed ex vivo and in immobilized animals in vivo, detecting IOSs of photoreceptor activity in living humans was cumbersome and time consuming. Here, we observed clear spatially and temporally resolved changes in the optical path length of the photoreceptor outer segment as a response to an optical stimulus in the living human eye. To witness these changes, we evaluated phase data obtained with a parallelized and computationally aberration-corrected optical coherence tomography system. The noninvasive detection of optical path length changes shows neuronal photoreceptor activity of single cones in living human retina, and therefore, it may provide diagnostic options in ophthalmology and neurology and could provide insights into visual phototransduction in humans.},
       url = {http://www.pnas.org/content/early/2016/10/10/1606428113.abstract},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.: A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67. Sci Rep, no. 6, pp. 27032, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
       title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
       journal = {Sci Rep},
       volume = {6},
       pages = {27032},
       note = {2045-2322
    Wang, Sijia
    Huttmann, Gereon
    Scholzen, Thomas
    Zhang, Zhenxi
    Vogel, Alfred
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal Article
    England
    Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
       abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
       ISSN = {2045-2322},
       DOI = {10.1038/srep27032},
       year = {2016},
       type = {Journal Article}
    }
    
  • Yasui, Ayako and Yamamoto, Manabu and Hirayama, Kumiko and Shiraki, Kunihiko and Theisen-Kunde, Dirk and Brinkmann, Ralf and Miura, Yoko and Kohno, Takeya: Retinal sensitivity after selective retina therapy (SRT) on patients with central serous chorioretinopathy. Graefe`s Archive for Clinical and Experimental Ophthalmology, pp. 1-12, 2016
    BibTeX Link
    @article{Yasui2016,
       author = {Yasui, Ayako and Yamamoto, Manabu and Hirayama, Kumiko and Shiraki, Kunihiko and Theisen-Kunde, Dirk and Brinkmann, Ralf and Miura, Yoko and Kohno, Takeya},
       title = {Retinal sensitivity after selective retina therapy (SRT) on patients with central serous chorioretinopathy},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
       pages = {1-12},
       abstract = {To assess retinal sensitivity after selective retina therapy (SRT) in patients with central serous chorioretinopathy (CSCR).},
       ISSN = {1435-702X},
       url = {http://dx.doi.org/10.1007/s00417-016-3441-8},
       year = {2016},
       type = {Journal Article}
    }
    
  • Pfeiffer, Tom and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Kolb, Jan-Phillip and Eibl, Matthias and Huber, Robert: Flexible A-scan rate MHz OCT: computational downscaling by coherent averaging. Proc. SPIE, no. 9697, pp. 96970S-96970S-5, 15
    BibTeX Link
    @inproceedings{Pfeiffer2016,
       author = {Pfeiffer, Tom and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Kolb, Jan-Phillip and Eibl, Matthias and Huber, Robert},
       title = {Flexible A-scan rate MHz OCT: computational downscaling by coherent averaging},
    Year={2015}
       journal = {Proc. SPIE},
       volume = {9697},
       pages = {96970S-96970S-5},
       note = {10.1117/12.2214788},
       abstract = {In order to realize fast OCT-systems with adjustable line rate, we investigate averaging of image data from an FDML based MHz-OCT-system. The line rate can be reduced in software and traded in for increased system sensitivity and image quality. We compare coherent and incoherent averaging to effectively scale down the system speed of a 3.2 MHz FDML OCT system to around 100 kHz in postprocessing. We demonstrate that coherent averaging is possible with MHz systems without special interferometer designs or digital phase stabilisation. We show OCT images of a human finger knuckle joint in vivo with very high quality and deep penetration.},
       url = {http://dx.doi.org/10.1117/12.2214788},
       type = {Conference Proceedings}
    }
    
  • Kolb, Jan Philip and Klein, Thomas and Eibl, Matthias and Pfeiffer, Tom and Wieser, Wolfgang and Huber, Robert: Megahertz FDML laser with up to 143nm sweep range for ultrahigh resolution OCT at 1050nm. in Proc. SPIE, no. 9697, pp. 969703-969703-8, 16
    BibTeX Link
    @inproceedings{Kolb2016,
       author = {Kolb, Jan Philip and Klein, Thomas and Eibl, Matthias and Pfeiffer, Tom and Wieser, Wolfgang and Huber, Robert},
       title = {Megahertz FDML laser with up to 143nm sweep range for ultrahigh resolution OCT at 1050nm},
       booktitle = {Proc. SPIE},
       volume = {9697},
    
       pages = {969703-969703-8},
       year={2016}
       note = {10.1117/12.2214758},
       abstract = {We present a new design of a Fourier Domain Mode Locked laser (FDML laser), which provides a new record in sweep range at ~1μm center wavelength: At the fundamental sweep rate of 2x417 kHz we reach 143nm bandwidth and 120nm with 4x buffering at 1.67MHz sweep rate. The latter configuration of our system is characterized: The FWHM of the point spread function (PSF) of a mirror is 5.6μm (in tissue). Human in vivo retinal imaging is performed with the MHz laser showing more details in vascular structures. Here we could measure an axial resolution of 6.0μm by determining the FWHM of specular reflex in the image. Additionally, challenges related to such a high sweep bandwidth such as water absorption are investigated.},
       url = {http://dx.doi.org/10.1117/12.2214758},
       type = {Conference Proceedings}
    }
    
  • Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy. Journal of Biomedical Optics, no. 21, pp. 078001-078001, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {7},
       pages = {078001-078001},
       note = {10.1117/1.JBO.21.7.078001},
       abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
       ISSN = {1083-3668},
       year = {2016},
       type = {Journal Article}
    }
    
  • Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogel, Alfred: Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery. American Physical Society,Phys. Rev. B, no. 94, pp. 1-19, 2016
    BibTeX Link
    @article{Linz2016,
       author = {Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogel, Alfred},
       title = {Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery},
       journal = {American Physical Society,Phys. Rev. B},
       volume = { 94},
       number = {2},
       pages = {1-19},
       url = {http://link.aps.org/doi/10.1103/PhysRevB.94.024113},
       year = {2016},
       type = {Journal Article}
    }
    
  • Ranjbar, Mahdy and Brinkmann, Max Philipp and Tura, Aysegül and Rudolf, Martin and Miura, Yoko and Grisanti, Salvatore: Ranibizumab interacts with the VEGF-A/VEGFR-2 signaling pathway in human RPE cells at different levels. Cytokine, no. 83, pp. 210-216, 2016
    BibTeX Link
    @article{Ranjbar2016,
       author = {Ranjbar, Mahdy and Brinkmann, Max Philipp and Tura, Aysegül and Rudolf, Martin and Miura, Yoko and Grisanti, Salvatore},
       title = {Ranibizumab interacts with the VEGF-A/VEGFR-2 signaling pathway in human RPE cells at different levels},
       journal = {Cytokine},
       volume = {83},
       pages = {210-216},
       abstract = {Vascular endothelial growth factor (VEGF) secreted by the retinal pigment epithelium (RPE) plays an important role in ocular homeostasis, but also in diseases, most notably age-related macular degeneration (AMD). To date, anti-VEGF drugs like ranibizumab have been shown to be most effective in treating these pathologic conditions. However, clinical trials suggest that the RPE could degenerate and perish through anti-VEGF treatment. Herein, we evaluated possible pathways and outcomes of the interaction between ranibizumab and human RPE cells (ARPE-19). Results indicate that ranibizumab affects the VEGF-A metabolism in RPE cells from an extra- as well as intracellular site. The drug is taken up into the cells, with the VEGF receptor 2 (VEGFR-2) being involved, and decreases VEGF-A protein levels within the cells as well as extracellularly. Oxidative stress plays a key role in various inflammatory disorders of the eye. Our results suggest that oxidative stress inhibits RPE cell proliferation. This anti-proliferative effect on RPE cells is significantly enhanced through ranibizumab, which does not inhibit RPE cell proliferation substantially in absence of relevant oxidative stress. Therefore, we emphasize that anti-VEGF treatment should be selected carefully in AMD patients with preexistent extensive RPE atrophy.},
       keywords = {Ranibizumab
    RPE
    VEGF-A
    VEGFR-2
    Oxidative stress},
       ISSN = {1043-4666},
       url = {http://www.sciencedirect.com/science/article/pii/S1043466616300722},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam-Peters, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs: Heartbeat OCT and Motion-Free 3D In Vivo Coronary Artery Microscopy. JACC: Cardiovascular Imaging, no. 9, pp. 622-623, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam-Peters, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs},
       title = {Heartbeat OCT and Motion-Free 3D In Vivo Coronary Artery Microscopy},
       journal = {JACC: Cardiovascular Imaging},
       volume = {9},
       number = {5},
       pages = {622-623},
       note = {10.1016/j.jcmg.2015.08.010},
       abstract = {Intravascular optical coherence tomography (IV-OCT) has gained widespread use over the past few years, offering highly detailed images of coronary artery pathologies and interventions (1). In contrast to the cross-sectional view, longitudinal sections and 3-dimensional (3D) renderings are affected by cardiac motion artifacts and undersampling, complicating interpretation and measurements (2). We developed Heartbeat OCT, a new OCT method that achieves up to 4,000 frames/s imaging speed for isotropically sampled volume datasets acquired within the diastolic phase of 1 cardiac cycle to restore 3D IV-OCT image fidelity. In this research letter, we present the first in vivo data acquired with this new experimental technology.},
       keywords = {OPTICAL COHERENCE TOMOGRAPHY; 2ND },
       ISSN = {1936-878X},
       DOI = {10.1016/j.jcmg.2015.08.010},
       year = {2016},
       type = {Journal Article}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert: Two-photon microscopy using fiber-based nanosecond excitation. Biomedical Optics Express, no. 7, pp. 2432-2440, 2016
    BibTeX Link
    @article{Karpf2016,
       author = {Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert},
       title = {Two-photon microscopy using fiber-based nanosecond excitation},
       journal = {Biomedical Optics Express},
       volume = {7},
       number = {7},
       pages = {2432-2440},
       abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
       keywords = {Fiber optics imaging
    Nonlinear optics, fibers
    Lasers, fiber
    Fluorescence microscopy
    Nonlinear microscopy},
       DOI = {10.1364/BOE.7.002432},
       year = {2016},
       type = {Journal Article}
    }
    
  • Huber, Robert and Dworak, Lars and Moser, Jacques E. and Grätzel, Michael and Wachtveitl, Josef: Beyond Vibrationally Mediated Electron Transfer: Coherent Phenomena Induced by Ultrafast Charge Separation. The Journal of Physical Chemistry C, no. 120, pp. 8534-8539, 2016
    BibTeX Link
    @article{Huber2016,
       author = {Huber, Robert and Dworak, Lars and Moser, Jacques E. and Grätzel, Michael and Wachtveitl, Josef},
       title = {Beyond Vibrationally Mediated Electron Transfer: Coherent Phenomena Induced by Ultrafast Charge Separation},
       journal = {The Journal of Physical Chemistry C},
       volume = {120},
       number = {16},
       pages = {8534-8539},
       ISSN = {1932-7447},
       DOI = {10.1021/acs.jpcc.6b02012},
       year = {2016},
       type = {Journal Article}
    }
    
  • Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter: Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation). no. 9691, pp. 969116-969116-1,
    BibTeX Link
    @inproceedings{Pieper2016,
       author = {Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter},
       title = {Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation)},
       volume = {9691},
       pages = {969116-969116-1},
       note = {10.1117/12.2209054},
       abstract = {Mucus transport is essential to remove inhaled particles and pathogens from the lung. Impaired removal of mucus often results in worsening of lung diseases. To understand the mechanisms of mucus transport and to monitor the impact of therapeutic strategies, it is essential to visualize airways and mucus in living animals without disturbing transport processes by intubation or surgically opening the airways. We developed a custom-built optical coherence microscope (OCM) providing a lateral and axial resolution of approximately 1.5 µm with a field of view of 2 mm at up to 150 images/s. Images of the intact trachea and its mucus transport were recorded in anesthetized spontaneously breathing mice. NaCl solution (0.9% and 7%) or Lipopolysaccharide were applied intranasally. OCM resolved detailed structure of the trachea and enabled measuring the airway surface liquid (ASL) thickness through the tracheal wall. Without stimulation, the amount of ASL was only a few µm above the epithelium and remained constant. After intranasal application of 30 µl saline at different concentrations, an early fast cough-like fluid removal with velocities higher than 1 mm/s was observed that removed a high amount of liquid. The ASL thickness increased transiently and quickly returned to levels before stimulation. In contrast to saline, application of Lipopolysaccharide induced substantial mucus release and an additional slow mucus transport by ciliary beating (around 100 µm/s) towards the larynx was observed. In conclusion, OCM is appropriate unique tool to study mechanisms of mucus transport in the airways and effects of therapeutic interventions in living animals.},
       url = {http://dx.doi.org/10.1117/12.2209054},
       type = {Conference Proceedings}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon: Aberration-free volumetric high-speed imaging of in vivo retina. Scientific Reports, no. 6, pp. 1-11, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon},
       title = {Aberration-free volumetric high-speed imaging of in vivo retina},
       journal = {Scientific Reports},
       volume = {6},
       pages = {1-11},
       url = {http://dx.doi.org/10.1038/srep35209},
       year = {2016},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: Imaging vascular dynamics in human retina using full-field swept-source optical coherence tomography (Conference Presentation). no. 9697, pp. 96970E-96970E-1,
    BibTeX Link
    @inproceedings{Spahr2016,
       author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {Imaging vascular dynamics in human retina using full-field swept-source optical coherence tomography (Conference Presentation)},
       volume = {9697},
       pages = {96970E-96970E-1},
       note = {10.1117/12.2214303},
       abstract = {We demonstrate a new non-invasive method to assess the functional condition of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. Motion of retinal tissue, that is induced by expansion of the vessels therein, is measured with an accuracy of about 10 nm. The pulse shape of arterial and venous pulsation, their temporal delay as well as the frequency dependent pulse propagation through the capillary bed are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
       url = {http://dx.doi.org/10.1117/12.2214303},
       type = {Conference Proceedings}
    }
    
  • Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.: Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. SOP2, 2016
    BibTeX Link
    @article{Pieper2016,
       author = {Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.},
       title = {Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {SOP2},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584654},
       year = {2016},
       type = {Journal Article}
    }
    
  • Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.: Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b. J Cancer Res Clin Oncol, no. 142, pp. 1261-71, 2016
    BibTeX Link
    @article{Maushagen2016,
       author = {Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.},
       title = {Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b},
       journal = {J Cancer Res Clin Oncol},
       volume = {142},
       number = {6},
       pages = {1261-71},
       note = {1432-1335
    Maushagen, Regina
    Reers, Stefan
    Pfannerstill, Ann-Christin
    Hahlbrock, Angelina
    Stauber, Roland
    Rahmanzadeh, Ramtin
    Rades, Dirk
    Pries, Ralph
    Wollenberg, Barbara
    Journal Article
    Germany
    J Cancer Res Clin Oncol. 2016 Jun;142(6):1261-71. doi: 10.1007/s00432-016-2150-3. Epub 2016 Apr 1.},
       abstract = {PURPOSE: Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to beta-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet. METHODS: The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies. RESULTS: Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome. CONCLUSION: Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.},
       keywords = {Apoptosis
    Caspase 9b
    Caspases
    Head and neck cancer
    Paclitaxel},
       ISSN = {0171-5216},
       DOI = {10.1007/s00432-016-2150-3},
       year = {2016},
       type = {Journal Article}
    }
    
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 1: Physikalische Grundlagen. Augenheilkunde up2date, no. 6, pp. 289-300, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 1: Physikalische Grundlagen},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {289-300},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-113337},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 2: Praktische Aspekte und Anwendung. Augenheilkunde up2date, no. 6, pp. 305-320, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 2: Praktische Aspekte und Anwendung},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {305-320},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-117459},
       year = {2016},
       type = {Journal Article}
    }
    
  • Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.: Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept. Cell Physiol Biochem, no. 38, pp. 737-47, 2016
    BibTeX Link
    @article{Ranjbar2016,
       author = {Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.},
       title = {Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept},
       journal = {Cell Physiol Biochem},
       volume = {38},
       number = {2},
       pages = {737-47},
       note = {1421-9778
    Ranjbar, Mahdy
    Brinkmann, Max Philipp
    Zapf, Dorinja
    Miura, Yoko
    Rudolf, Martin
    Grisanti, Salvatore
    Journal Article
    Switzerland
    Cell Physiol Biochem. 2016;38(2):737-47. doi: 10.1159/000443030. Epub 2016 Feb 15.},
       abstract = {BACKGROUND/AIMS: VEGF-A is induced by oxidative stress, and functions as a survival factor for various cell types, including retinal pigment epithelial (RPE) cells. Anti-vascular endothelial growth factor (VEGF) drugs like aflibercept and bevacizumab have shown to be most effective in treating neovascular age-related macular degeneration (AMD), however uptake of the drugs might lead to interference with cell physiology. Herein, we evaluated the significance of the Fc receptor (FcR) within this context and moreover explored the impact of VEGF inhibition under normal conditions as well as under oxidative stress, in terms of potential adverse effects. METHODS: ARPE-19 (human RPE) cells were treated with aflibercept and bevacizumab in presence or absence of H2O2 as oxidative stress stimulus. After 24h cells were evaluated for drug uptake, VEGF-A expression and secretion, levels of intracellular reactive oxygen species (ROS) as well as cell proliferation. Experiments were repeated with cells being pre-incubated with an FcR inhibitor prior to drug application. RESULTS: Both drugs inhibited extracellular levels of VEGF-A and were taken up into the RPE, resulting in significantly reduced intracellular levels of VEGF-A. When oxidative stress was applied, intracellular ROS levels in cells treated with both drugs rose, and cell proliferation was reduced. Prior incubation with the FcR inhibitor lessened the uptake of bevacizumab, but not aflibercept into RPE cells, and simultaneously enhanced cell survival under oxidative stress conditions. CONCLUSIONS: Our results indicate that uptake and accumulation of aflibercept and bevacizumab within RPE cells affect the intracellular VEGF-A metabolism negatively, leading to a biologically relevant reduced cell survival under oxidative stress. The FcR plays a substantial role in the uptake of bevacizumab, but not aflibercept, which allows an enhanced RPE cell survival through FcR blockage in an environment dominated by oxidative stress, as clinically significant for various inflammatory retinal disorders.},
       ISSN = {1015-8987},
       DOI = {10.1159/000443030},
       year = {2016},
       type = {Journal Article}
    }
    
  • Kang, S. and Park, Y. G. and Kim, J. R. and Seifert, E. and Theisen-Kunde, D and Brinkman, R and Roh, Y. J.: Selective Retina Therapy in Patients With Chronic Central Serous Chorioretinopathy: A Pilot Study. Medicine (Baltimore), no. 95, pp. e2524, 2016
    BibTeX Link
    @article{Kang1016,
       author = {Kang, S. and Park, Y. G. and Kim, J. R. and Seifert, E. and Theisen-Kunde, D and Brinkman, R and Roh, Y. J.},
       title = {Selective Retina Therapy in Patients With Chronic Central Serous Chorioretinopathy: A Pilot Study},
       journal = {Medicine (Baltimore)},
       volume = {95},
       number = {3},
       pages = {e2524},
       note = {1536-5964
    Kang, Seungbum
    Park, Young Gun
    Kim, Jae Ryun
    Seifert, Eric
    Dirk, Theisen-Kunde
    Ralf, Brinkmann
    Roh, Young Jung
    Journal Article
    United States
    Medicine (Baltimore). 2016 Jan;95(3):e2524. doi: 10.1097/MD.0000000000002524.},
       abstract = {We evaluated visual outcomes, changes of maximum macular thickness (MMT) and subretinal fluid (SRF), and safety in patients with chronic central serous chorioretinopathy (CSC) after treatment with selective retina therapy (SRT). Retrospective cohort study of patients with chronic CSC presenting to a university-based hospital from January 2014 through January 2015 was conducted. A total of 12 eyes of 12 patients with chronic CSC lasting for at least 3 months was recruited. The follow-up period ranged from 3 to 12 months. Following evaluation of test spots at temporal arcades, SRT (Q-switched neodymium-doped yttrium lithium fluoride [Nd:YLF] laser; wavelength, 527 nm, pulse duration, 1.7 microsececond) was applied to the surrounding areas of leakage observed on fluorescein angiogram and/or pigment epithelial detachment (PED). Changes in best-correct visual acuity (BCVA), MMT, and SRF and macular sensitivity (MS) by microperimetry (MP) were evaluated. Eyes received treatment in a mean of 3.83 spots at the pulse energy of 65 to 90 muJ. Mean BCVA (logMAR) improved from 0.23 +/- 0.12 at baseline to 0.14 +/- 0.13 at 3 months. MMT decreased from 341.4 +/- 85.5 mum at baseline to 236.0 +/- 57.9 mum at 3 months. SRF completely resolved in 75% (9 eyes) at 3 months. Large PEDs (2 eyes) were flattened at 3 months. Retreatment was performed in 4 eyes. MP showed no evidence of scotoma around SRT-treated lesions. SRT treatment targeting the surrounding area of leakage point showed favorable visual and structural outcomes in chronic CSC patients without the risk of scotoma.},
       ISSN = {0025-7974},
       DOI = {10.1097/md.0000000000002524},
       year = {2016},
       type = {Journal Article}
    }
    
  • Park, Y. G. and Kim, J. R. and Kang, S. and Seifert, E. and Theisen-Kunde, D. and Brinkmann, R. and Roh, Y. J.: Safety and efficacy of selective retina therapy (SRT) for the treatment of diabetic macular edema in Korean patients. Graefes Arch Clin Exp Ophthalmol, 2016
    BibTeX Link
    @article{Park2016,
       author = {Park, Y. G. and Kim, J. R. and Kang, S. and Seifert, E. and Theisen-Kunde, D. and Brinkmann, R. and Roh, Y. J.},
       title = {Safety and efficacy of selective retina therapy (SRT) for the treatment of diabetic macular edema in Korean patients},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       note = {1435-702x
    Park, Young Gun
    Kim, Jae Ryun
    Kang, Seungbum
    Seifert, Eric
    Theisen-Kunde, Dirk
    Brinkmann, Ralf
    Roh, Young-Jung
    Journal article
    Graefes Arch Clin Exp Ophthalmol. 2016 Jan 23.},
       abstract = {PURPOSE: Selective retina therapy (SRT) stimulates retinal pigment epithelium (RPE) cell migration and proliferation into irradiated areas. The objective of this study was to evaluate the efficacy and safety of SRT in Korean patients with clinically significant diabetic macular edema (DME). METHODS: Prospective non-randomized interventional case series study. Twenty-three eyes of 21 patients with clinically significant DME were treated with SRT and followed for 6 months. Patients underwent an evaluation of best corrected visual acuity (BCVA) in Early Treatment Diabetic Retinopathy Study (ETDRS) letters. Microperimetry was employed to measure macular sensitivity within the central 10 degrees field, and the central macular thickness (CMT) and maximum macular thickness (MMT) were measured. RESULTS: An improvement in BCVA of one to two ETDRS lines was observed in 41.2 % of patients and an improvement of greater than two lines in 29.4 %. Although there was no significant change in CMT (P > 0.05), MMT decreased from 465.8 +/- 87.4 mum to 434.3 +/- 83.9 mum (P = 0.006), and mean macular sensitivity increased from 20.8 +/- 3.4dB to 22.5 +/- 3.5dB (P = 0.02). CONCLUSIONS: The gains in BCVA and improvement in macular sensitivity demonstrated that SRT may be used as an effective and safe treatment modality in Korean patients with clinically significant DME.},
       keywords = {Diabetic macular edema
    Dosimetry
    Microperimetry
    Retinal pigment epithelium
    Selective retina therapy},
       ISSN = {0721-832x},
       DOI = {10.1007/s00417-015-3262-1},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Lange, B. and Cordes, J. and Brinkmann, R.: Stone/tissue differentiation for holmium laser lithotripsy using autofluorescence. Lasers Surg Med, no. 47, pp. 737-44, 2015
    BibTeX Link
    @article{Lange2015,
       author = {Lange, B. and Cordes, J. and Brinkmann, R.},
       title = {Stone/tissue differentiation for holmium laser lithotripsy using autofluorescence},
       journal = {Lasers Surg Med},
       volume = {47},
       number = {9},
       pages = {737-44},
       note = {1096-9101
    Lange, Birgit
    Cordes, Jens
    Brinkmann, Ralf
    Journal Article
    United States
    Lasers Surg Med. 2015 Nov;47(9):737-44. doi: 10.1002/lsm.22418. Epub 2015 Sep 22.},
       abstract = {BACKGROUND AND OBJECTIVES: Holmium laser lithotripsy is a safe and effective method to disintegrate urinary stones of all compositions in an endoscopic procedure. However, handling and safety could be improved by a real-time feedback system permanently monitoring the position of the treatment fiber. The laser is fired only when the fiber is identified as being placed in front of stone. This work evaluates the potential of fluorescence detection with an excitation wavelength of 532 nm for this purpose. MATERIALS AND METHODS: A fiber-based fluorescence measurement was set-up to acquire autofluorescence signals from several human renal calculi, artificial stones, and porcine tissue samples (renal calix and ureter). Three different approaches were investigated. First, experiments were performed with a pulsed laser source with a wavelength of 532 nm, pulse energy 36.5 +/- 1 muJ, pulse duration 1.2 +/- 0.5 nanoseconds, and a repetition rate of 1 kHz with 15 urinary concretions. In the second step, a series of measurements on 42 human urinary calculi samples was carried out using low power continuous wave excitation of 0.4 +/- 0.1 mW. Fluorescence was also measured simultaneously to stone fragmentation by holmium laser pulses (pulse energy 240 +/- 50 mJ, repetition rate 10 Hz). Finally, a modulated excitation/detection scheme (lock-in technique) was implemented to render fluorescence detection insensitive to white background light. RESULTS: Unlike porcine renal calix, ureter, and artificial stone human urinary calculi show a strong fluorescence signal when excited with 532 nm. With pulsed excitation on urinary stone (20,000 +/- 11,000) counts were registered at 587 nm with the CCD-array of a grating spectrometer in an integration time of 50 milliseconds. Tissue gave lower count rates of </=(5,500 +/- 1,100) even with longer integration times (500 milliseconds/1 second). With a cw excitation power of 0.4 mW (13,000 +/- 11,000) counts were registered in an integration time of 200 milliseconds at 587 nm (porcine renal calix: (770 +/- 340)). Modulated excitation (66 Hz) with an average power of 0.3 mW and detection with a photodiode resulted in a lock-in amplifier signal of 1.5-4.3V on stone (background and skin: <0.5V). CONCLUSION: With the lock-in technique, autofluorescence from stones can be detected with only the average excitation power of a green aiming beam overlaid to the Ho:YAG-laser beam (power </= 1 mW). Since tissue shows very little autofluorescence when excited with 532 nm, this fluorescence signal enables monitoring of the correct position of the treatment fiber during ureteroscopic procedures. Lasers Surg. Med. 47:737-744, 2015. (c) 2015 Wiley Periodicals, Inc.},
       keywords = {Holmium laser
    feedback control
    fluorescence
    laser lithotripsy},
       ISSN = {0196-8092},
       DOI = {10.1002/lsm.22418},
       year = {2015},
       type = {Journal Article}
    }
    
  • Niu, C. J. and Fisher, C. and Scheffler, K. and Wan, R. and Maleki, H. and Liu, H. and Sun, Y. and A. Simmons, C. and Birngruber, R. and Lilge, L.: Polyacrylamide gel substrates that simulate the mechanical stiffness of normal and malignant neuronal tissues increase protoporphyin IX synthesis in glioma cells. Journal of Biomedical Optics, no. 20,
    BibTeX Link
    @article{Niu2015,
       author = {Niu, C. J. and Fisher, C. and Scheffler, K. and Wan, R. and Maleki, H. and Liu, H. and Sun, Y. and A. Simmons, C. and Birngruber, R. and Lilge, L.},
       title = {Polyacrylamide gel substrates that simulate the mechanical stiffness of normal and malignant neuronal tissues increase protoporphyin IX synthesis in glioma cells},
       journal = {Journal of Biomedical Optics},
       volume = {20},
       number = {9},
       DOI = {10.1117/1.JBO.20.9.098002},
      
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs: Heartbeat OCT: in vivo intravascular megahertz-optical coherence tomography. Biomedical Optics Express, no. 6, pp. 5021-5032, 2015
    BibTeX Link
    @article{HU_2015_Wang,
       author = {Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs},
       title = {Heartbeat OCT: in vivo intravascular megahertz-optical coherence tomography},
       journal = {Biomedical Optics Express},
       volume = {6},
       number = {12},
       pages = {5021-5032},
       abstract = {Cardiac motion artifacts, non-uniform rotational distortion and undersampling affect the image quality and the diagnostic impact of intravascular optical coherence tomography (IV-OCT). In this study we demonstrate how these limitations of IV-OCT can be addressed by using an imaging system that we called &#x201C;Heartbeat OCT&#x201D;, combining a fast Fourier Domain Mode Locked laser, fast pullback, and a micromotor actuated catheter, designed to examine a coronary vessel in less than one cardiac cycle. We acquired in vivo data sets of two coronary arteries in a porcine heart with both Heartbeat OCT, working at 2.88 MHz A-line rate, 4000 frames/s and 100 mm/s pullback speed, and with a commercial system. The in vivo results show that Heartbeat OCT provides faithfully rendered, motion-artifact free, fully sampled vessel wall architecture, unlike the conventional IV-OCT data. We present the Heartbeat OCT system in full technical detail and discuss the steps needed for clinical translation of the technology.},
       keywords = {Fiber optics imaging
    Three-dimensional image acquisition
    Medical optics instrumentation
    Scanners
    Endoscopic imaging
    Medical and biological imaging
    Optical coherence tomography},
       DOI = {10.1364/BOE.6.005021},
       year = {2015},
       type = {Journal Article}
    }
    
  • Steiner, Patrick and Ebneter, Andreas and Berger, Lieselotte Erika and Zinkernagel, Martin and Považay, Boris and Meier, Christoph and Kowal, Jens H. and Framme, Carsten and Brinkmann, Ralf and Wolf, Sebastian and Sznitman, Raphael: Time-Resolved Ultra–High Resolution Optical Coherence Tomography for Real-Time Monitoring of Selective Retina TherapyTime-Resolved Ultra–High Resolution OCT During SRT. Investigative Ophthalmology & Visual Science, no. 56, pp. 6654-6662, 2015
    BibTeX Link
    @article{Steiner2015,
       author = {Steiner, Patrick and Ebneter, Andreas and Berger, Lieselotte Erika and Zinkernagel, Martin and Považay, Boris and Meier, Christoph and Kowal, Jens H. and Framme, Carsten and Brinkmann, Ralf and Wolf, Sebastian and Sznitman, Raphael},
       title = {Time-Resolved Ultra–High Resolution Optical Coherence Tomography for Real-Time Monitoring of Selective Retina TherapyTime-Resolved Ultra–High Resolution OCT During SRT},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {56},
       number = {11},
       pages = {6654-6662},
       note = {10.1167/iovs.15-17151},
       abstract = {Abstract Purpose: Selective retina therapy (SRT) is a novel treatment for retinal pathologies, solely targeting the RPE. During SRT, the detection of an immediate tissue reaction is challenging, as tissue effects remain limited to intracellular RPE photodisruption. Time-resolved ultra-high axial resolution optical coherence tomography (OCT) is thus evaluated for the monitoring of dynamic optical changes at and around the RPE during SRT. Methods: An experimental OCT system with an ultra-high axial resolution of 1.78 μm was combined with an SRT system and time-resolved OCT M-scans of the target area were recorded from four patients undergoing SRT. Optical coherence tomography scans were analyzed and OCT morphology was correlated with findings in fluorescein angiography, fundus photography, and cross-sectional OCT. Results: In cases in which the irradiation caused RPE damage proven by fluorescein angiography, the lesions were well discernible in time-resolved OCT images but remained invisible in fundus photography and cross-sectional OCT acquired after treatment. If RPE damage was introduced, all applied SRT pulses led to detectable signal changes in the time-resolved OCT images. The extent of optical signal variation seen in the OCT data appeared to scale with the applied SRT pulse energy. Conclusions: The first clinical results proved that successful SRT irradiation induces detectable changes in the OCT M-scan signal while it remains invisible in conventional ophthalmoscopic imaging. Thus, real-time high-resolution OCT is a promising modality to monitor and analyze tissue effects introduced by selective retina therapy and may be used to guide SRT in an automatic feedback mode (www.swissmedic.ch number, 2011-MD-0006).},
       ISSN = {1552-5783},
       DOI = {10.1167/iovs.15-17151},
       year = {2015},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Hillmann, Dierck and Huttmann, Gereon: Functional Microangiography of in vivo human retina by Full-Field OCT. Investigative Ophthalmology & Visual Science, no. 56, pp. 5974-5974, 2015
    BibTeX Link
    @article{Spahr2015,
       author = {Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Hillmann, Dierck and Huttmann, Gereon},
       title = {Functional Microangiography of in vivo human retina by Full-Field OCT},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {56},
       number = {7},
       pages = {5974-5974},
       abstract = { PurposeOCT based functional microangiography of the retina requires high speed acquisition of a large number of volumetric datasets. Imaging speed of conventional scanning OCT devices is limited by the applicable radiant power and the mechanics used to scan the focused beam over the desired field of view. Full-Field Swept-Source OCT (FF-SS-OCT) resolves both issues, using an areal illumination, which dramatically increases the allowed amount of radiation, and an ultrafast camera for a highly parallelized acquisition.  MethodsThe retina of healthy volunteers was illuminated with wavelengths between 816 and 867 nm by the extended beam of a tunable laser (Broadsweeper, Superlum). Retinal irradiance was below the maximum permissable exposure (MPE). Light backscattered from the retina was imaged onto an ultrafast CMOS camera (SA-Z, Photron), where it interfered with an extended reference beam. From a series of interference images at different wavelengths, volumetric OCT images of the retina were reconstructed.  ResultsWe demonstrate in vivo retinal imaging at 9.9 billion voxels per second (40 million A-scans/s with 256 axial pixels). Sacrificing depth resolution by reducing the number of axial pixels, the A-scan rate was increased to more than 1 billion A-scans per second. FF-SS-OCT allowed imaging of all important retinal structures with good quality at unprecedented imaging speed (see fig. 1). Fast volumetric imaging at up to 3000 volumes/s was used to visualize small capillaries and to analyze the pulsation of retinal arteries and veins (see fig. 2). Imaging time for an area of 4 mm x 2 mm (896 x 368 A-scans) was only 316 µs. The high volume rate and the inherent phase stability enabled quantitative measurement of the change of retinal thickness due to blood pulsation with approx. 10 nm precision. A delay of the venous pulsation with respect to the arteries was observed (approx. 11 ms). The amplitudes of higher frequency components of the venous pulsation were considerably attenuated.  ConclusionsFF-SS-OCT provides fast volumetric imaging of the retina with good image quality. The capillary network can be analyzed with high spatial and temporal resolution. Analysis of retinal pulsation may provide information on pathological changes of vessels and capillaries. Angiographic OCT acquired with the FF-SS-OCT setup. Functional angiography showing the pulsation of retinal artery and vein.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2015},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography. Optics Letters, no. 40, pp. 4771-4774, 2015
    BibTeX Link Link
    @article{Spahr2015,
       author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography},
       journal = {Optics Letters},
       volume = {40},
       number = {20},
       pages = {4771-4774},
       abstract = {We demonstrate a new noninvasive method to assess biomechanical properties of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. The motion of retinal tissue that is induced by expansion of the vessels therein is measured with an accuracy of about 10 nm. The pulse shapes of arterial and venous pulsations, their temporal delays, as well as the frequency-dependent pulse propagation through the capillary bed, are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
       keywords = {Optical coherence tomography
    Ophthalmology
    Time-resolved imaging
    Functional monitoring and imaging},
       DOI = {10.1364/OL.40.004771},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-40-20-4771},
       year = {2015},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Baade, A. and Schlott, K. and Hesse, C. and Caliebe, A. and Roider, J. and Brinkmann, R.: Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions. Transl Vis Sci Technol, no. 4, pp. 9, 2015
    BibTeX Link
    @article{Koinzer2015,
       author = {Koinzer, S. and Baade, A. and Schlott, K. and Hesse, C. and Caliebe, A. and Roider, J. and Brinkmann, R.},
       title = {Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions},
       journal = {Transl Vis Sci Technol},
       volume = {4},
       number = {5},
       pages = {9},
       note = {Koinzer, Stefan
    Baade, Alexander
    Schlott, Kerstin
    Hesse, Carola
    Caliebe, Amke
    Roider, Johann
    Brinkmann, Ralf
    Journal article
    Transl Vis Sci Technol. 2015 Oct 6;4(5):9. eCollection 2015 Oct.},
       abstract = {PURPOSE: Conventional retinal photocoagulation produces irregular lesions and does not allow reliable control of ophthalmoscopically invisible lesions. We applied automatically controlled retinal photocoagulation, which allows to apply uniform lesions without titration, and aimed at five different predictable lesion intensities in a study on rabbit eyes. METHODS: A conventional 532-nm photocoagulation laser was used in combination with a pulsed probe laser. They facilitated real-time fundus temperature measurements and automatic exposure time control for different predefined time/temperature dependent characteristics (TTC). We applied 225 control lesions (exposure time 200 ms) and 794 TTC lesions (5 intensities, exposure times 7-800 ms) in six rabbit eyes with variable laser power (20-66.4 mW). Starting after 2 hours, we examined fundus color and optical coherence tomographic (OCT) images over 3 months and classified lesion morphologies according to a seven-stage OCT classifier. RESULTS: Visibility rates in funduscopy (OCT) after 2 hours were 17% (68%) for TTC intensity group 1, 38% (90%) for TTC group 2 and greater than 94% (>98%) for all consecutive groups. TTC groups 1 through 4 correlated to increasing morphological lesion intensities and increasing median funduscopic and OCT diameters. Group 5 lesions were as large as, but more intense than group 4 lesions. CONCLUSIONS: Automatic, temperature controlled photocoagulation allows to apply predictable subvisible, mild, or moderate lesions without manual power titration. TRANSLATIONAL RELEVANCE: The technique will facilitate standardized, automatically controlled low and early treatment of diabetic retinopathy study (ETDRS) intensity photocoagulation independently of the treating physician, the treated eye and lesion location.},
       keywords = {Oct
    animal model
    laser photocoagulation
    optoacoustics
    real-time temperature measurement
    spectral domain
    sub-visible},
       ISSN = {2164-2591 (Print)
    2164-2591},
       DOI = {10.1167/tvst.4.5.9},
       year = {2015},
       type = {Journal Article}
    }
    
  • Park, Young Gun and Kang, Seungbum and Brinkmann, Ralf and Roh, Young-Jung: A Comparative Study of Retinal Function in Rabbits after Panretinal Selective Retina Therapy versus Conventional Panretinal Photocoagulation. Journal of Ophthalmology, no. 2015, pp. 8, 2015
    BibTeX Link
    @article{Park2015,
       author = {Park, Young Gun and Kang, Seungbum and Brinkmann, Ralf and Roh, Young-Jung},
       title = {A Comparative Study of Retinal Function in Rabbits after Panretinal Selective Retina Therapy versus Conventional Panretinal Photocoagulation},
       journal = {Journal of Ophthalmology},
       volume = {2015},
       pages = {8},
       DOI = {10.1155/2015/247259},
       url = {http://dx.doi.org/10.1155/2015/247259},
       year = {2015},
       type = {Journal Article}
    }
    
  • Mohler, Kathrin J. and Draxinger, Wolfgang and Klein, Thomas and Kolb, Jan Philip and Wieser, Wolfgang and Haritoglou, Christos and Kampik, Anselm and Fujimoto, James G. and Neubauer, Aljoscha S. and Huber, Robert and Wolf, Armin: Combined 60° Wide-Field Choroidal Thickness Maps and High-Definition En Face Vasculature Visualization Using Swept-Source Megahertz OCT at 1050 nm60° High-Definition MHz-OCT Imaging of the Choroid. Investigative Ophthalmology & Visual Science, no. 56, pp. 6284-6293, 2015
    BibTeX Link
    @article{HU_Mohler_2015,
    author = {Mohler, Kathrin J. and Draxinger, Wolfgang and Klein, Thomas and Kolb, Jan Philip and Wieser, Wolfgang and Haritoglou, Christos and Kampik, Anselm and Fujimoto, James G. and Neubauer, Aljoscha S. and Huber, Robert and Wolf, Armin},
    title = {Combined 60° Wide-Field Choroidal Thickness Maps and High-Definition En Face Vasculature Visualization Using Swept-Source Megahertz OCT at 1050 nm60° High-Definition MHz-OCT Imaging of the Choroid},
    journal = {Investigative Ophthalmology & Visual Science},
    volume = {56},
    number = {11},
    pages = {6284--6293},
    year = {2015},
    doi = {10.1167/iovs.15-16670},
    eprint = {/data/Journals/IOVS/934564/i1552-5783-56-11-6284.pdf}
    }
  • Klinger, A. and Krapf, L. and Orzekowsky-Schroeder, R. and Koop, N. and Vogel, A. and Huttmann, G.: Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation. J Biomed Opt, no. 20, pp. 116001, 2015
    BibTeX Link
    @article{Klinger2017,
       author = {Klinger, A. and Krapf, L. and Orzekowsky-Schroeder, R. and Koop, N. and Vogel, A. and Huttmann, G.},
       title = {Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation},
       journal = {J Biomed Opt},
       volume = {20},
       number = {11},
       pages = {116001},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.20.11.116001},
       year = {2015},
       type = {Journal Article}
    }
    
  • Ansari, R. and Buj, C. and Pieper, M. and Konig, P. and Schweikard, A. and Huttmann, G.: Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy. Opt Express, no. 23, pp. 23217-24, 2015
    BibTeX Link
    @article{Ansari2015,
       author = {Ansari, R. and Buj, C. and Pieper, M. and Konig, P. and Schweikard, A. and Huttmann, G.},
       title = {Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy},
       journal = {Opt Express},
       volume = {23},
       number = {18},
       pages = {23217-24},
       note = {1094-4087
    Ansari, Rehman
    Buj, Christian
    Pieper, Mario
    Konig, Peter
    Schweikard, Achim
    Huttmann, Gereon
    Journal Article
    United States
    Opt Express. 2015 Sep 7;23(18):23217-24. doi: 10.1364/OE.23.023217.},
       abstract = {Motile cilia perform a range of important mechanosensory and chemosensory functions, along with expulsion of mucus and inhaled pathogens from the lungs. Here we demonstrate that spectral domain optical coherence phase microscopy (SD-OCPM), which combines the principles of optical coherence tomography (OCT) and confocal microscopy, is particularly well-suited for characterization of both morphology and the ciliary dynamics of mouse trachea. We present micro-anatomical images of mouse trachea, where different cell types can be clearly visualized. The phase contrast, which measures the sub-nanometer changes in axial optical pathlength is used to determine the frequency and direction of cilia beatings.},
       ISSN = {1094-4087},
       DOI = {10.1364/oe.23.023217},
       year = {2015},
       type = {Journal Article}
    }
    
  • Zarnescu, Livia and Leung, Michael C. and Abeyta, Michael and Sudkamp, Helge and Baer, Thomas and Behr, Barry and Ellerbee, Audrey K.: Label-free characterization of vitrification-induced morphology changes in single-cell embryos with full-field optical coherence tomography. Journal of Biomedical Optics, no. 20, pp. 096004-096004, 2015
    BibTeX Link
    @article{Zarnescu2015,
       author = {Zarnescu, Livia and Leung, Michael C. and Abeyta, Michael and Sudkamp, Helge and Baer, Thomas and Behr, Barry and Ellerbee, Audrey K.},
       title = {Label-free characterization of vitrification-induced morphology changes in single-cell embryos with full-field optical coherence tomography},
       journal = {Journal of Biomedical Optics},
       volume = {20},
       number = {9},
       pages = {096004-096004},
       note = {10.1117/1.JBO.20.9.096004},
       abstract = {Abstract.  Vitrification is an increasingly popular method of embryo cryopreservation that is used in assisted reproductive technology. Although vitrification has high post-thaw survival rates compared to other freezing techniques, its long-term effects on embryo development are still poorly understood. We demonstrate an application of full-field optical coherence tomography (FF-OCT) to visualize the effects of vitrification on live single-cell (2 pronuclear) mouse embryos without harmful labels. Using FF-OCT, we observed that vitrification causes a significant increase in the aggregation of structures within the embryo cytoplasm, consistent with reports in literature based on fluorescence techniques. We quantify the degree of aggregation with an objective metric, the cytoplasmic aggregation (CA) score, and observe a high degree of correlation between the CA scores of FF-OCT images of embryos and of fluorescence images of their mitochondria. Our results indicate that FF-OCT shows promise as a label-free assessment of the effects of vitrification on embryo mitochondria distribution. The CA score provides a quantitative metric to describe the degree to which embryos have been affected by vitrification and could aid clinicians in selecting embryos for transfer.},
       ISSN = {1083-3668},
       DOI = {10.1117/1.JBO.20.9.096004},
       url = {http://dx.doi.org/10.1117/1.JBO.20.9.096004},
       year = {2015},
       type = {Journal Article}
    }
    
  • Wieser, Wolfgang and Klein, Thomas and Draxinger, Wolfgang and Huber, Robert: Fully automated 1.5 MHz FDML laser with more than 100mW output power at 1310 nm. in Proc. SPIE, no. 9541, pp. 954116-954116-7, 2015
    BibTeX Link
    @inproceedings{HU_2015_Wieser_a,
    author = {Wieser, Wolfgang and Klein, Thomas and Draxinger, Wolfgang and Huber, Robert},
    title = { Fully automated 1.5 MHz FDML laser with more than 100mW output power at 1310 nm },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9541},
    pages = {954116-954116-7},
    year = {2015},
    doi = {10.1117/12.2183431}
    }
  • Reznicek, Lukas and Kolb, Jan P. and Klein, Thomas and Mohler, Kathrin J. and Wieser, Wolfgang and Huber, Robert and Kernt, Marcus and Märtz, Josef and Neubauer, Aljoscha S.: Wide-Field Megahertz OCT Imaging of Patients with Diabetic Retinopathy. Journal of Diabetes Research, no. 2015, Article ID 305084, pp. 1-5, 2015
    BibTeX Link
    @article{Reznicek2015,
       author = {Reznicek, Lukas and Kolb, Jan P. and Klein, Thomas and Mohler, Kathrin J. and Wieser, Wolfgang and Huber, Robert and Kernt, Marcus and Märtz, Josef and Neubauer, Aljoscha S.},
       title = {Wide-Field Megahertz OCT Imaging of Patients with Diabetic Retinopathy},
       journal = {Journal of Diabetes Research},
       volume = {2015, Article ID 305084},
       pages = {1-5},
       DOI = {10.1155/2015/305084},
       url = {http://dx.doi.org/10.1155/2015/305084},
       year = {2015},
       type = {Journal Article}
    
    }
    
    
  • Kolb, Jan Philip and Klein, Thomas and Wieser, Wolfgang and Draxinger, Wolfgang and Huber, Robert: High definition in vivo retinal volumetric video rate OCT at 0.6 Giga-voxels per second. in Proc. SPIE, no. 9541, pp. 95410Z-95410Z-8, 2015
    BibTeX Link
    @inproceedings{HU_2015_Kolb_d,
    author = {Kolb, Jan Philip and Klein, Thomas and Wieser, Wolfgang and Draxinger, Wolfgang and Huber, Robert},
    title = {High definition in vivo retinal volumetric video rate OCT at 0.6 Giga-voxels per second },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9541},
    pages = {95410Z-95410Z-8},
    year = {2015},
    doi = {10.1117/12.2183768}
    }
  • Karpf, Sebastian and Eibl, Matthias and Huber, Robert: Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser. in Proc. SPIE, no. 9536, pp. 953616-953616-5, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_c,
    author = {Karpf, Sebastian and Eibl, Matthias and Huber, Robert},
    title = { Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953616-953616-5},
    year = {2015},
    doi = {10.1117/12.2183854}
    }
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Hyperspectral stimulated Raman microscopy with two fiber laser sources. in Proc. SPIE, no. 9536, pp. 953604-953604-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Eibl_a,
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = {Hyperspectral stimulated Raman microscopy with two fiber laser sources },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953604-953604-6},
    year = {2015},
    doi = {10.1117/12.2183822}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers. in Proc. SPIE, no. 9541, pp. 95410F-95410F-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_b,
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = { Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers },
    booktitle = {Proc. SPIE },
    volume = {9541},
    type = {Conference Proceedings},
    pages = {95410F-95410F-6},
    year = {2015},
    doi = {10.1117/12.2183859}
    }
  • Horstmann, J. and Spahr, H. and Buj, C. and Munter, M. and Brinkmann, R.: Full-field speckle interferometry for non-contact photoacoustic tomography. Phys Med Biol, no. 60, pp. 4045-58, 2015
    BibTeX Link
    @article{Horstmann2015,
       author = {Horstmann, J. and Spahr, H. and Buj, C. and Munter, M. and Brinkmann, R.},
       title = {Full-field speckle interferometry for non-contact photoacoustic tomography},
       journal = {Phys Med Biol},
       volume = {60},
       number = {10},
       pages = {4045-58},
       note = {1361-6560
       abstract = {A full-field speckle interferometry method for non-contact and prospectively high speed Photoacoustic Tomography is introduced and evaluated as proof of concept. Thermoelastic pressure induced changes of the objects topography are acquired in a repetitive mode without any physical contact to the object. In order to obtain high acquisition speed, the object surface is illuminated by laser pulses and imaged onto a high speed camera chip. In a repetitive triple pulse mode, surface displacements can be acquired with nanometre sensitivity and an adjustable sampling rate of e.g. 20 MHz with a total acquisition time far below one second using kHz repetition rate lasers. Due to recurring interferometric referencing, the method is insensitive to thermal drift of the object due to previous pulses or other motion. The size of the investigated area and the spatial and temporal resolution of the detection are scalable. In this study, the approach is validated by measuring a silicone phantom and a porcine skin phantom with embedded silicone absorbers. The reconstruction of the absorbers is presented in 2D and 3D. The sensitivity of the measurement with respect to the photoacoustic detection is discussed. Potentially, Photoacoustic Imaging can be brought a step closer towards non-anaesthetized in vivo imaging and new medical applications not allowing acoustic contact, such as neurosurgical monitoring or burnt skin investigation.},
       ISSN = {0031-9155},
       DOI = {10.1088/0031-9155/60/10/4045},
       year = {2015},
       type = {Journal Article}
    }
    
  • Framme, C. and Walter, A. and Berger, L. and Prahs, P. and Alt, C. and Theisen-Kunde, D. and Kowal, J. and Brinkmann, R.: Selective Retina Therapy in Acute and Chronic-Recurrent Central Serous Chorioretinopathy. Ophthalmologica, no. 234, pp. 177-88, 2015
    BibTeX Link
    @article{Framme2015,
       author = {Framme, C. and Walter, A. and Berger, L. and Prahs, P. and Alt, C. and Theisen-Kunde, D. and Kowal, J. and Brinkmann, R.},
       title = {Selective Retina Therapy in Acute and Chronic-Recurrent Central Serous Chorioretinopathy},
       journal = {Ophthalmologica},
       volume = {234},
       number = {4},
       pages = {177-88},
       note = {1423-0267
    Framme, Carsten
    Walter, Andreas
    Berger, Lieselotte
    Prahs, Philipp
    Alt, Clemens
    Theisen-Kunde, Dirk
    Kowal, Jens
    Brinkmann, Ralf
    Journal Article
    Research Support, Non-U.S. Gov't
    Switzerland
    Ophthalmologica. 2015;234(4):177-88. doi: 10.1159/000439188. Epub 2015 Sep 15.},
       abstract = {PURPOSE: Selective retina therapy (SRT), the confined laser heating and destruction of retinal pigment epithelial cells, has been shown to treat acute types of central serous chorioretinopathy (CSC) successfully without damaging the photoreceptors and thus avoiding laser-induced scotoma. However, a benefit of laser treatment for chronic forms of CSC is questionable. In this study, the efficacy of SRT by means of the previously used 1.7-micros and shorter 300-ns pulse duration was evaluated for both types of CSC, also considering re-treatment for nonresponders. MATERIAL AND METHODS: In a two-center trial, 26 patients were treated with SRT for acute (n = 10) and chronic-recurrent CSC (n = 16). All patients presented with subretinal fluid (SRF) in OCT and leakage in fluorescein angiography (FA). SRT was performed using a prototype SRT laser system (frequency-doubled Q-switched Nd:YLF-laser, wavelength 527 nm) with adjustable pulse duration. The following irradiation settings were used: a train of 30 laser pulses with a repetition rate of 100 Hz and pulse durations of 300 ns and 1.7 micros, pulse energy 120-200 microJ, retinal spot size 200 microm. Because SRT lesions are invisible, FA was always performed 1 h after treatment to demonstrate laser outcome (5-8 single spots in the area of leakage). In cases where energy was too low, as indicated by missing FA leakage, energy was adjusted and the patient re-treated immediately. Observation intervals were after 4 weeks and 3 months. In case of nonimprovement of the disease after 3 months, re-treatment was considered. RESULTS: Of 10 patients with active CSC that presents focal leakage in FA, 5 had completely resolved fluid after 4 weeks and all 10 after 3 months. Mean visual acuity increased from 76.6 ETDRS letters to 85.0 ETDRS letters 3 months after SRT. Chronic-recurrent CSC was characterized by less severe SRF at baseline in OCT and weaker leakage in FA than in acute types. Visual acuity changed from baseline 71.6 to 72.8 ETDRS letters after 3 months. At this time, SRF was absent in 3 out of 16 patients (19%), FA leakage had come to a complete stop in 6 out of 16 patients (38%). In 6 of the remaining chronic CSC patients, repeated SRT with higher pulse energy was considered because of persistent leakage activity. After the re-treatment, SRF resolved completely in 5 patients (83.3%) after only 25 days. CONCLUSION: SRT showed promising results in treating acute CSC, but was less effective in chronic cases. Interestingly, re-treatment resulted in enhanced fluid resolution and dry conditions after a considerably shorter time in most patients. Therefore, SRT including re-treatment if necessary might be a valuable CSC treatment alternative even in chronic-recurrent cases.},
       ISSN = {0030-3755},
       DOI = {10.1159/000439188},
       year = {2015},
       type = {Journal Article}
    }
    
  • Jirauschek, Christian and Huber, Robert: Wavelength shifting of intra-cavity photons: Adiabatic wavelength tuning in rapidly wavelength-swept lasers. Biomedical Optics Express, no. 6, pp. 2448-2465, 2015
    BibTeX Link
    @article{Jirauschek2015,
       author = {Jirauschek, Christian and Huber, Robert},
       title = {Wavelength shifting of intra-cavity photons: Adiabatic wavelength tuning in rapidly wavelength-swept lasers},
       journal = {Biomedical Optics Express},
       volume = {6},
       number = {7},
       pages = {2448-2465},
       abstract = {We analyze the physics behind the newest generation of rapidly wavelength tunable sources for optical coherence tomography (OCT), retaining a single longitudinal cavity mode during operation without repeated build up of lasing. In this context, we theoretically investigate the currently existing concepts of rapidly wavelength-swept lasers based on tuning of the cavity length or refractive index, leading to an altered optical path length inside the resonator. Specifically, we consider vertical-cavity surface-emitting lasers (VCSELs) with microelectromechanical system (MEMS) mirrors as well as Fourier domain mode-locked (FDML) and Vernier-tuned distributed Bragg reflector (VT-DBR) lasers. Based on heuristic arguments and exact analytical solutions of Maxwell&#x02019;s equations for a fundamental laser resonator model, we show that adiabatic wavelength tuning is achieved, i.e., hopping between cavity modes associated with a repeated build up of lasing is avoided, and the photon number is conserved. As a consequence, no fundamental limit exists for the wavelength tuning speed, in principle enabling wide-range wavelength sweeps at arbitrary tuning speeds with narrow instantaneous linewidth.},
       keywords = {Laser theory
    Lasers, tunable
    Optical coherence tomography
    Vertical cavity surface emitting lasers},
       DOI = {10.1364/BOE.6.002448},
       url = {http://www.osapublishing.org/boe/abstract.cfm?URI=boe-6-7-2448},
       year = {2015},
       type = {Journal Article}
    }
    
  • Diddens-Tschoeke, Heyke C. and Hüttmann, Gereon and Gruber, Achim D. and Pottier, Roy H. and Hanken, Henning: Localized thermal tumor destruction using dye-enhanced photothermal tumor therapy. Lasers in Surgery and Medicine, pp. n/a-n/a, 2015
    BibTeX Link
    @article{Diddens-Tschoeke2015,
       author = {Diddens-Tschoeke, Heyke C. and Hüttmann, Gereon and Gruber, Achim D. and Pottier, Roy H. and Hanken, Henning},
       title = {Localized thermal tumor destruction using dye-enhanced photothermal tumor therapy},
       journal = {Lasers in Surgery and Medicine},
       pages = {n/a-n/a},
       keywords = {photothermal therapy
    naphthalocyanine derivative
    in-vivo
    laser
    dye-enhanced},
       ISSN = {1096-9101},
       DOI = {10.1002/lsm.22356},
       url = {http://dx.doi.org/10.1002/lsm.22356},
       year = {2015},
       type = {Journal Article}
    }
    
    
  • Huber, Robert: 4-D Real-Time Optical Coherence Tomography. Optics & Photonics News, no. 06, pp. 32-39, 2015
    BibTeX
    @article{Huber2015,
       author = {Huber, Robert},
       title = {4-D Real-Time Optical Coherence Tomography },
       journal = {Optics & Photonics News},
       volume = {06},
       pages = {32-39},
       url = {http://www.osa-opn.org/home/articles/volume_26/june_2015/features/4-d_real-time_optical_coherence_tomography/ 6/1/2015 3:19:13 PM },
       year = {2015},
       type = {Journal Article}
    }
    
  • Jirauschek, Christian and Huber, Robert: Modeling and analysis of polarization effects in Fourier domain mode-locked lasers. Optics Letters, no. 40, pp. 2385-2388, 2015
    BibTeX Link
    @article{Jirauschek2015,
       author = {Jirauschek, Christian and Huber, Robert},
       title = {Modeling and analysis of polarization effects in Fourier domain mode-locked lasers},
       journal = {Optics Letters},
       volume = {40},
       number = {10},
       pages = {2385-2388},
       abstract = {We develop a theoretical model for Fourier domain mode-locked (FDML) lasers in a non-polarization-maintaining configuration, which is the most widely used type of FDML source. This theoretical approach is applied to analyze a widely wavelength-swept FDML setup, as used for picosecond pulse generation by temporal compression of the sweeps. We demonstrate that good agreement between simulation and experiment can only be obtained by including polarization effects due to fiber bending birefringence, polarization mode dispersion, and cross-phase modulation into the theoretical model. Notably, the polarization dynamics are shown to have a beneficial effect on the instantaneous linewidth, resulting in improved coherence and thus compressibility of the wavelength-swept FDML output.},
       DOI = {10.1364/OL.40.002385},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-40-10-2385},
       year = {2015},
       type = {Journal Article}
    }
    
  • Apiou-Sbirlea, Gabriela and Tearney, Guillermo J. and Birngruber, Reginald and Hasan, Tayyaba and Anderson, Richard Rox: Anatomy and physiology of translation: the academic research imperative. Clinical Investigation, no. 5, pp. 797-804, 2015
    BibTeX Link
    @article{Apiou-Sbirlea2015,
       author = {Apiou-Sbirlea, Gabriela and Tearney, Guillermo J. and Birngruber, Reginald and Hasan, Tayyaba and Anderson, Richard Rox},
       title = {Anatomy and physiology of translation: the academic research imperative},
       journal = {Clinical Investigation},
       volume = {5},
       number = {10},
       pages = {797-804},
       ISSN = {2041-6792},
       DOI = {10.4155/cli.15.46},
     ,
       year = {2015},
       type = {Journal Article}
    }
    
  • Buj, C and Horstmann, J and Münter, M and Brinkman, R: Speckle-based off-axis holographic detection for non-contact photoacoustic tomography. Current Directions in Biomedical Engineering, no. 1, pp. 356-360, 2015
    BibTeX
    @article{Buj2015,
       author = {Buj, C and Horstmann, J and Münter, M and Brinkman, R},
       title = {Speckle-based off-axis holographic detection for non-contact photoacoustic tomography},
       journal = {Current Directions in Biomedical Engineering},
       volume = {1},
       pages = {356-360},
       year = {2015},
       type = {Journal Article}
    }
    
  • Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogelmann, Hannes and Trickl, Thomas and Vogel, Alfred: Wavelength dependence of nanosecond infrared laser-induced breakdown in water: Evidence for multiphoton initiation via an intermediate state. Physical Review B, no. 91, pp. 134114, 2015
    BibTeX Link
    @article{Linz2015,
       author = {Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogelmann, Hannes and Trickl, Thomas and Vogel, Alfred},
       title = {Wavelength dependence of nanosecond infrared laser-induced breakdown in water: Evidence for multiphoton initiation via an intermediate state},
       journal = {Physical Review B},
       volume = {91},
       number = {13},
       pages = {134114},
       note = {PRB},
       DOI = {doi/10.1103/PhysRevB.91.134114},
       url = {http://link.aps.org/doi/10.1103/PhysRevB.91.134114},
       year = {2015},
       type = {Journal Article}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy. Nature Communications, no. 6, pp. 6784 1-6;, 2015
    BibTeX Link
    @Article{HU_2015_Karpf_a,
      Title                    = {A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy},
      Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
      Journal                  = {Nature Communications},
      Year                     = {2015},
      Volume = {6},
      pages = {6784 1--6};
      Doi                      = {10.1038/ncomms7784}
    }
    
  • Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.: Improved endoscopic optical coherence microscopy for imaging of humans airways in patients. Pneumologie, no. 69, pp. A49, 2015
    BibTeX Link
    @article{Schulz-Hildebrandt2015,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.},
       title = {Improved endoscopic optical coherence microscopy for imaging of humans airways in patients},
       journal = {Pneumologie},
       volume = {69},
       number = {07},
       pages = {A49},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0035-1556641},
       year = {2015},
       type = {Journal Article}
    }
    
  • Jan Philip Kolb and Thomas Klein and Corinna L. Kufner and Wolfgang Wieser and Aljoscha S. Neubauer and Huber, Robert: Ultra-widefield retinal MHz-OCT imaging with up to 100 degrees viewing angle. Biomed. Opt. Express, no. 6, pp. 1534-1552, May, 2015
    BibTeX Link
    @article{HU_2015_Kolb_c, 
    author = {Jan Philip Kolb and Thomas Klein and Corinna L. Kufner and Wolfgang Wieser and Aljoscha S. Neubauer and Huber, Robert}, 
    journal = {Biomed. Opt. Express}, 
    number = {5}, 
    pages = {1534--1552}, 
    title = {Ultra-widefield retinal MHz-OCT imaging with up to 100 degrees viewing angle}, 
    Keywords = {OCT},
    volume = {6}, 
    month = {May},
    year = {2015},
    doi = {10.1364/BOE.6.001534}
    }
    
    
    
  • Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.: Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67. Mol Pharm, 2015
    BibTeX Link
    @article{Wang2015,
       author = {Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.},
       title = {Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67},
       journal = {Mol Pharm},
       note = {1543-8392
    Wang, Sijia
    Huttmann, Gereon
    Zhang, Zhenxi
    Vogel, Alfred
    Birngruber, Reginald
    Tangutoori, Shifalika
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal article
    Mol Pharm. 2015 Aug 13.},
       abstract = {The selective inhibition of intracellular and nuclear molecules such as Ki-67 holds great promise for the treatment of cancer and other diseases. However, the choice of the target protein and the intracellular delivery of the functional agent remain crucial challenges. Main hurdles are (a) an effective delivery into cells, (b) endosomal escape of the delivered agents, and (c) an effective, externally triggered destruction of cells. Here we show a light-controlled two-step approach for selective cellular delivery and cell elimination of proliferating cells. Three different cell-penetrating nano constructs, including liposomes, conjugates with the nuclear localization sequence (NLS), and conjugates with the cell penetrating peptide Pep-1, delivered the light activatable antibody conjugate TuBB-9-FITC, which targets the proliferation associated protein Ki-67. HeLa cells were treated with the photosensitizer benzoporphyrin monoacid derivative (BPD) and the antibody constructs. In the first optically controlled step, activation of BPD at 690 nm triggered a controlled endosomal escape of the TuBB-9-FITC constructs. In more than 75% of Ki-67 positive, irradiated cells TuBB-9-FITC antibodies relocated within 24 h from cytoplasmic organelles to the cell nucleus and bound to Ki-67. After a second light irradiation at 490 nm, which activated FITC, cell viability decreased to approximately 13%. Our study shows an effective targeting strategy, which uses light-controlled endosomal escape and the light inactivation of Ki-67 for cell elimination. The fact that liposomal or peptide-assisted delivery give similar results leads to the additional conclusion that an effective mechanism for endosomal escape leaves greater variability for the choice of the delivery agent.},
       keywords = {endosomal entrapment
    liposome
    nanotechnology
    nuclear localization sequence (NLS)
    photodynamic therapy},
       ISSN = {1543-8384},
       DOI = {10.1021/acs.molpharmaceut.5b00260},
       year = {2015},
       type = {Journal Article}
    }
    
  • Kolb, Jan Philip and Klein, Thomas and Wieser, Wolfgang and Draxinger, Wolfgang and Huber, Robert: Full volumetric video rate OCT of the posterior eye with up to 195.2 volumes/s. in Proc. SPIE, no. 9312, pp. 931202-931202-7, 2015
    BibTeX Link
    @inproceedings{Kolb2015 ,
       author = {Kolb, Jan Philip and Klein, Thomas and Wieser, Wolfgang and Draxinger, Wolfgang and Huber, Robert },
       title = {Full volumetric video rate OCT of the posterior eye with up to 195.2 volumes/s},
       volume = {9312},
    Year={2015},
       pages = {931202-931202-7 },
       DOI = {10.1117/12.2077147 },
       booktitle = {Proc. SPIE },
       type = {Conference Proceedings}
    }
    
  • Kolb, Jan Philip and Schwarz, Philipp and Klein, Thomas and Wieser, Wolfgang and Huber, Robert: Dual parametric compounding approach for speckle reduction in OCT. in Proc. SPIE, no. 9312, pp. 93123G-93123G-7, 2015
    BibTeX Link
    @inproceedings{Kolb2015,
       author = {Kolb, Jan Philip and Schwarz, Philipp and Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
       title = {Dual parametric compounding approach for speckle reduction in OCT},
    Year={2015},
       volume = {9312},
       pages = {93123G-93123G-7},
       DOI = {10.1117/12.2077659},
       booktitle = {Proc. SPIE},
       type = {Conference Proceedings}
    }
    
  • Baade, A and Schwarzer, W and Koinzer, S and Schlott, K and Birngruber, R and Brinkman, R: Power-controlled temperature guided retinal photocoagulation. in Photonic West BIOS,
    BibTeX
    @inproceedings{Baade2015,
       author = {Baade, A and Schwarzer, W and Koinzer, S and Schlott, K and Birngruber, R and Brinkman, R},
       title = {Power-controlled temperature guided retinal photocoagulation },
       booktitle = {Photonic West BIOS},
       type = {Conference Proceedings}
    }
    
  • Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald: Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits. Investigative Ophthalmology & Visual Science, no. 56, pp. 5980-5980, 2015
    BibTeX
    @article{Hüttmann2015,
       author = {Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald},
       title = {Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {56},
       number = {7},
       pages = {5980-5980},
       ISSN = {1552-5783},
       year = {2015},
       type = {Journal Article}
    }
    
  • Han, Bing and Köhler, Karsten and Jungnickel, Kerstin and Mettin, Robert and Lauterborn, Werner and Vogel, Alfred: Dynamics of laser-induced bubble pairs. Journal of Fluid Mechanics, no. 771, pp. 706-742, 2015
    BibTeX Link
    @article{Han2015,
       author = {Han, Bing and Köhler, Karsten and Jungnickel, Kerstin and Mettin, Robert and Lauterborn, Werner and Vogel, Alfred},
       title = {Dynamics of laser-induced bubble pairs},
       journal = {Journal of Fluid Mechanics},
       volume = {771},
       pages = {706-742},
       keywords = {bubble dynamics,cavitation,jets},
       ISSN = {1469-7645},
       DOI = {doi:10.1017/jfm.2015.183},
       url = {http://dx.doi.org/10.1017/jfm.2015.183},
       year = {2015},
       type = {Journal Article}
    }
    
  • Niu, C. J. and Fisher, C. and Scheffler, K. and Wan, R. and Maleki, H. and Liu, H. and Sun, Y. and C, A. Simmons and Birngruber, R. and Lilge, L.: Polyacrylamide gel substrates that simulate the mechanical stiffness of normal and malignant neuronal tissues increase protoporphyin IX synthesis in glioma cells. J Biomed Opt, no. 20, pp. 098002, 2015
    BibTeX Link
    @article{Niu2015,
       author = {Niu, C. J. and Fisher, C. and Scheffler, K. and Wan, R. and Maleki, H. and Liu, H. and Sun, Y. and C, A. Simmons and Birngruber, R. and Lilge, L.},
       title = {Polyacrylamide gel substrates that simulate the mechanical stiffness of normal and malignant neuronal tissues increase protoporphyin IX synthesis in glioma cells},
       journal = {J Biomed Opt},
       volume = {20},
       number = {9},
       pages = {098002},
       note = {1560-2281
    Niu, Carolyn J
    Fisher, Carl
    Scheffler, Kira
    Wan, Rachel
    Maleki, Hoda
    Liu, Haijiao
    Sun, Yu
    A Simmons, Craig
    Birngruber, Reginald
    Lilge, Lothar
    Canadian Institutes of Health Research/Canada
    Journal Article
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2015 Sep;20(9):098002. doi: 10.1117/1.JBO.20.9.098002.},
       abstract = {Protoporphyrin IX (PPIX) produced following the administration of exogenous 5d-aminolevulinic acid is clinically approved for photodynamic therapy and fluorescence-guided resection in various jurisdictions around the world. For both applications, quantification of PPIX forms the basis for accurate therapeutic dose calculation and identification of malignant tissues for resection. While it is well established that the PPIX synthesis and accumulation rates are subject to the cell's biochemical microenvironment, the effect of the physical microenvironment, such as matrix stiffness, has received little attention to date. Here we studied the proliferation rate and PPIX synthesis and accumulation in two glioma cell lines U373 and U118 cultured under five different substrate conditions, including the conventional tissue culture plastic and polyacrylamide gels that simulated tissue stiffness of normal brain (1 kPa) and glioblastoma tumors (12 kPa). We found that the proliferation rate increased with substrate stiffness for both cell lines, but not in a linear fashion. PPIX concentration was significantly higher in cells cultured on tissue-simulating gels than on the much stiffer tissue culture plastic for both cell lines. These findings, albeit preliminary, suggest that the physical microenvironment might be an important determinant of tumor aggressiveness and PPIX synthesis in glioma cells.},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.20.9.098002},
       year = {2015},
       type = {Journal Article}
    }
    
  • Kim, H. D. and Han, J. W. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.: Functional evaluation using multifocal electroretinogram after selective retina therapy with a microsecond-pulsed laser. Invest Ophthalmol Vis Sci, no. 56, pp. 122-31, 2015
    BibTeX Link
    @article{Kim2015,
       author = {Kim, H. D. and Han, J. W. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.},
       title = {Functional evaluation using multifocal electroretinogram after selective retina therapy with a microsecond-pulsed laser},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {56},
       number = {1},
       pages = {122-31},
       note = {1552-5783
    Kim, Hoon Dong
    Han, Jung Woo
    Ohn, Young-Hoon
    Brinkmann, Ralf
    Park, Tae Kwann
    Journal Article
    United States
    Invest Ophthalmol Vis Sci. 2014 Dec 11;56(1):122-31. doi: 10.1167/iovs.14-15132.},
       abstract = {PURPOSE: To evaluate the changes of retinal function with multifocal electroretinogram (mfERG), and estimate the association between functional and structural changes after selective retina therapy (SRT) with microsecond-pulsed laser in comparison to continuous wave laser photocoagulation (cwPC). METHODS: Selective retina therapy and cwPC were applied with 10 x 10 shots and 1/2 lesion-width on the retina in the right and left eyes of 20 healthy Chinchilla Bastard rabbits, respectively. Optical coherence tomography (OCT), fundus fluorescein angiography (FFA), and mfERG were performed before, and on days 1, 7, and 30 after both laser treatments. The mean ratios of amplitudes and implicit times of N1 and P1 from eight hexagons covering laser-treated retinal lesions/total retina were measured. Histology was obtained after killing three rabbits at each time period to observe the anatomic changes after both laser treatments. RESULTS: The mean ratios of amplitudes of N1 and P1 in SRT lesions did not change significantly for 30 days after laser treatment. Only subtle reductions of the mean ratios of N1 and P1 amplitudes on day 1, thereafter the amplitudes showed the trend to recover toward baseline values. Histology and OCT revealed temporary and reversible morphologic changes after SRT, which restored to normal within 1 month. However, the mean ratios of N1 amplitudes on days 7 and 30 (P = 0.010, P < 0.001, respectively), and P1 amplitudes on days 7 and 30 (P < 0.001, P < 0.001, respectively) declined significantly in cwPC lesions compared with baseline. Disorganization and atrophic changes were identified on histology and OCT after cwPC. CONCLUSIONS: The results suggest that SRT preserved retinal function as well as anatomical structure after treatment.},
       keywords = {continuous wave laser photocoagulation (cwPC)
    multifocal electroretinogram (mfERG)
    selective retina therapy (SRT)},
       ISSN = {0146-0404},
       DOI = {10.1167/iovs.14-15132},
       year = {2015},
       type = {Journal Article}
    }
    

2014

  • Evers, M. and Ha, L. and Welford, D. and Birngruber, R. and Manstein, D.: Wavelength-Dependence of Fractional Ablations in Biological Materials Using a Tunable Cr2+:Znse/S Infrared Laser. Lasers in Surgery and Medicine, no. 46, pp. 38-39, 2014
    BibTeX Link
    @article{Evers2014,
       author = {Evers, M. and Ha, L. and Welford, D. and Birngruber, R. and Manstein, D.},
       title = {Wavelength-Dependence of Fractional Ablations in Biological Materials Using a Tunable Cr2+:Znse/S Infrared Laser},
       journal = {Lasers in Surgery and Medicine},
       volume = {46},
       pages = {38-39},
       note = {Suppl. 25
    Ad2hq
    119
    Times Cited:0
    Cited References Count:0},
       ISSN = {0196-8092},
       url = {<Go to ISI>://WOS:000333055800109},
       year = {2014},
       type = {Journal Article}
    }
    
  • Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert: A 4-D OCT Engine with 1 GVoxel/s. Optics and Photonics News, no. 25, pp. 36, OSA, dec,, 2014
    BibTeX
    @Article{HU_2014_Wieser_b,
      Title                    = {{A 4-D OCT Engine with 1 GVoxel/s}},
      Author                   = {Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Optics and Photonics News},
      Year                     = {2014},
    
      Month                    = dec,
      Number                   = {12},
      Pages                    = {36 },
      Volume                   = {25},
    
      Publisher                = {OSA},
      Url                      = { http://www.osa-opn.org/home/articles/volume_25/december_2014/extras/a_4-d_oct_engine_with_1_gvoxel_s/#.VcH21Pl5raw}
    }
  • Rudolf, M. and Mohi, A. and Dettbarn, M. C. and Miura, Y. and Aherrahrou, Z. and Ranjbar, M. and Mutus, B. and Knobloch, J. K.: Detection of esterified cholesterol in murine Bruch`s membrane wholemounts with a perfringolysin O-based cholesterol marker. Invest Ophthalmol Vis Sci, no. 55, pp. 4759-67, 2014
    BibTeX Link
    @article{Rudolf2014,
       author = {Rudolf, M. and Mohi, A. and Dettbarn, M. C. and Miura, Y. and Aherrahrou, Z. and Ranjbar, M. and Mutus, B. and Knobloch, J. K.},
       title = {Detection of esterified cholesterol in murine Bruch's membrane wholemounts with a perfringolysin O-based cholesterol marker},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {55},
       number = {8},
       pages = {4759-67},
       note = {1552-5783
    Rudolf, Martin
    Mohi, Armin
    Dettbarn, Marie C
    Miura, Yoko
    Aherrahrou, Zouhair
    Ranjbar, Mahdy
    Mutus, Bulent
    Knobloch, Johannes K M
    Journal Article
    Research Support, Non-U.S. Gov't
    United States
    Invest Ophthalmol Vis Sci. 2014 Jul 1;55(8):4759-67. doi: 10.1167/iovs.14-14311.},
       abstract = {PURPOSE: To investigate the effects of Bruch's membrane (BrM) neutral lipid deposition in mouse models and its significance to aging and age-related macular degeneration, it is essential to reliably detect small quantities of neutral lipids including esterified cholesterol (EC). In chorioretinal sections and BrM wholemounts, we tested a novel fluorescent cholesterol marker based on the bacterial toxin perfringolysin O (PFO) and compared results with those obtained with the classic cholesterol dye filipin. METHODS: An engineered plasmid containing the specific cholesterol binding domain (D4) of PFO fused to green fluorescent protein (GFP) was expressed in cultured E. coli, isolated, purified, and concentrated. A total of 150 BrM-choroid wholemounts and chorioretinal sections of 11- to 13-month-old ApoE(null) mice were prepared and stained with PFO/D4-GFP or filipin for EC. Samples were examined by epifluorescence microscopy. RESULTS: The fluorescence intensity of PFO/D4-GFP was strong, stable, and, if small quantities of EC were present, superior to filipin. In all specimens, we could sharply locate the PFO/D4-GFP signal to BrM. A semiquantitative evaluation of BrM lipid deposition is possible by measuring PFO/D4-GFP fluorescence intensity. CONCLUSIONS: The use of PFO/D4-GFP allowed a robust and direct detection of EC in aged murine BrM. In wholemount samples, its strong and stable fluorescence facilitated a semiquantitative evaluation of BrM-EC content over a large area. The patterns of EC deposition in murine BrM wholemounts are comparable with findings in human BrM wholemounts. Perfringolysin O/D4-GFP could be an important tool for investigating the effects of BrM lipid deposition in mouse models.},
       keywords = {Aging/*metabolism/pathology
    Animals
    Bacterial Toxins
    Bruch Membrane/*metabolism/ultrastructure
    Cells, Cultured
    Cholesterol Esters/*metabolism
    Clostridium perfringens
    Disease Models, Animal
    Feasibility Studies
    Female
    Hemolysin Proteins/*diagnostic use
    Humans
    Macular Degeneration/*diagnosis/metabolism
    Mice
    Mice, Inbred C57BL
    Microscopy, Electron, Transmission},
       ISSN = {0146-0404},
       DOI = {10.1167/iovs.14-14311},
       year = {2014},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Bajorat, S. and Hesse, C. and Caliebe, A. and Bever, M. and Brinkmann, R. and Roecken, C. and Roider, J.: Calibration of histological retina specimens after fixation in Margo`s solution and paraffin embedding to in-vivo dimensions, using photography and optical coherence tomography. Graefes Arch Clin Exp Ophthalmol, no. 252, pp. 145-53, 2014
    BibTeX Link
    @article{Koinzer2014,
       author = {Koinzer, S. and Bajorat, S. and Hesse, C. and Caliebe, A. and Bever, M. and Brinkmann, R. and Roecken, C. and Roider, J.},
       title = {Calibration of histological retina specimens after fixation in Margo's solution and paraffin embedding to in-vivo dimensions, using photography and optical coherence tomography},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {252},
       number = {1},
       pages = {145-53},
       note = {1435-702x
    Koinzer, Stefan
    Bajorat, Sandra
    Hesse, Carola
    Caliebe, Amke
    Bever, Marco
    Brinkmann, Ralf
    Roecken, Christoph
    Roider, Johann
    Journal Article
    Research Support, Non-U.S. Gov't
    Germany
    Graefes Arch Clin Exp Ophthalmol. 2014 Jan;252(1):145-53. doi: 10.1007/s00417-013-2457-6. Epub 2013 Sep 14.},
       abstract = {BACKGROUND: The extent of retinal tissue deformation by histological processing needs to be separately measured for every workup protocol. This work presents a simple approach for its quantitative assessment, and shows lateral and axial scaling factors for a common protocol. We calibrated histological measurements by in-vivo photographic and optical coherence tomographic (OCT) measurements, using retinal photocoagulation lesions as calibration markers. METHODS: We evaluated four rabbit eyes that were examined histologically after fixation in Margo's solution (1 % paraformaldehyde:1.25 % glutaraldehyde), isopropanol dehydration, paraffin embedding and hematoxylin and eosin staining. Distances between 51 pairs of laser lesions were compared in photographs and on histological slides. Retinal thickness measurements were performed at 15 anatomically defined sites in these eyes, and related to anatomically matched OCT thickness measurements of six different rabbit eyes. RESULTS: We found that the ratio of histological over photographic lesion distances was 1.17 (95 % CI 1.13-1.22), indicating 17 % lateral retinal stretching or expansion by the processing. Thickness measurements in histology were 65.6 % of the in-vivo thickness as measured in OCT, indicating 1/3 axial tissue compression or shrinkage. CONCLUSIONS: We provide an analysis of retinal tissue deformation after fixation in Margo's solution and paraffin embedding. In spite of protocol optimization for reduced tissue deformation, the workup caused 1/3 axial compression/shrinkage and 17 % lateral elongation, which was unexpected. We show a simple way how to calibrate retina specimens by fundus photography and OCT, two methods that are readily available to most ophthalmologists. Our findings underline the necessity to calibrate specimens prior to morphometry.},
       keywords = {Animals
    Calibration
    *Histological Techniques
    Laser Coagulation
    *Paraffin Embedding
    Photography/*methods
    Rabbits
    *Retina
    *Tissue Fixation
    Tomography, Optical Coherence/*methods},
       ISSN = {0721-832x},
       DOI = {10.1007/s00417-013-2457-6},
       year = {2014},
       type = {Journal Article}
    }
    
  • Rohde, Ingo and Brinkmann, Ralf: Gain broadening and mode-locking in overcoupled second harmonic Q-switched microsecond pulses. Journal of Optics, no. 16, pp. 105209, 2014
    BibTeX
    @article{Rohde2014,
       author = {Rohde, Ingo and Brinkmann, Ralf},
       title = {Gain broadening and mode-locking in overcoupled second harmonic Q-switched microsecond pulses},
       journal = {Journal of Optics},
       volume = {16},
       number = {10},
       pages = {105209},
       abstract = {An intracavity frequency doubled, Q-switched Nd:YLF emitting at a wavelength of 527 nm was designed with the goal to temporally stretch the Q-switched pulses up to some microseconds at pulse energies of several millijoules. With different resonator configurations pulse durations between 12 μ s and 3 μ s with energies of 1 mJ–4.5 mJ have been achieved, which is demanded for an application in ophthalmology. For tighter intracavity foci and high pump power, however, strong power modulations by trains of picosecond pulses on the rear flank of the microsecond pulses were observed, indicating the occurrence of cascading nonlinearities and mode-locking. Simultaneously a significant increase of the fundamental spectrum up to 5 nm was found. A similar effect, which is referred to as gain broadening, has previously been observed by using ppKTP for intracavity second harmonic generation. This is, to the best of our knowledge, the first observation of this effect with unpoled second harmonic media.},
       ISSN = {2040-8986},
       url = {http://stacks.iop.org/2040-8986/16/i=10/a=105209},
       year = {2014},
       type = {Journal Article}
    }
    
  • Vogel, A and Freidank, S and Linz, N: Alternativen zur Femtosekundentechnologie: UV Subnanosekunden-pulse und Ringfoki für LASIK Flaperzeugung (at press). Ophthalomologe, no. 111, 2014
    BibTeX
    @article{Vogel2014,
       author = {Vogel, A and Freidank, S and Linz, N },
       title = {Alternativen zur Femtosekundentechnologie: UV Subnanosekunden-pulse und Ringfoki für LASIK Flaperzeugung (at press)},
       journal = {Ophthalomologe },
       volume = {111},
       number = {6},
       year = {2014},
       type = {Journal Article}
    }
    
  • Li, Z. X. and Roussakis, E. and Koolen, P. G. L. and Ibrahim, A. M. S. and Kim, K. and Rose, L. F. and Wu, J. and Nichols, A. J. and Baek, Y. and Birngruber, R. and Apiou-Sbirlea, G. and Matyal, R. and Huang, T. and Chan, R. and Lin, S. J. and Evans, C. L.: Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage. Biomedical Optics Express, no. 5, pp. 3748-3764, 2014
    BibTeX Link
    @article{Li2014,
       author = {Li, Z. X. and Roussakis, E. and Koolen, P. G. L. and Ibrahim, A. M. S. and Kim, K. and Rose, L. F. and Wu, J. and Nichols, A. J. and Baek, Y. and Birngruber, R. and Apiou-Sbirlea, G. and Matyal, R. and Huang, T. and Chan, R. and Lin, S. J. and Evans, C. L.},
       title = {Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage},
       journal = {Biomedical Optics Express},
       volume = {5},
       number = {11},
       pages = {3748-3764},
       ISSN = {2156-7085},
       DOI = {10.1364/Boe.5.003748},
      
       year = {2014},
       type = {Journal Article}
    }
    
  • Buj, C and Horstmann, J and Münter, M and Brinkmann, R: Speckle-based holographic detection for non-contact Photoacoustic Tomography. in 48th annual conference of the German Society for Biomedical Engineering, no. 59, pp. 844-847,
    BibTeX
    @inproceedings{Buj14,
       author = {Buj, C and Horstmann, J and Münter, M and Brinkmann, R},
       title = {Speckle-based holographic detection for non-contact Photoacoustic Tomography},
       booktitle = {48th annual conference of the German Society for Biomedical Engineering},
       volume = {59},
       pages = {844-847},
       type = {Conference Proceedings}
    }
    
    
  • Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino: Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography. European Archives of Oto-Rhino-Laryngology, pp. 1-7, 2014
    BibTeX Link
    @article{Guder2014,
       author = {Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino},
       title = {Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography},
       journal = {European Archives of Oto-Rhino-Laryngology},
       pages = {1-7},
       keywords = {Optical coherence tomography
    Tympanic membrane
    Chronic myringitis},
       ISSN = {0937-4477},
       DOI = {10.1007/s00405-014-3373-z},
       url = {http://dx.doi.org/10.1007/s00405-014-3373-z},
       year = {2014},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Caliebe, A. and Portz, L. and Saeger, M. and Miura, Y. and Schlott, K. and Brinkmann, R. and Roider, J.: Comprehensive detection, grading, and growth behavior evaluation of subthreshold and low intensity photocoagulation lesions by optical coherence tomographic and infrared image analysis. Biomed Res Int, no. 2014, pp. 492679, 2014
    BibTeX Link
    @article{Koinzer2014,
       author = {Koinzer, S. and Caliebe, A. and Portz, L. and Saeger, M. and Miura, Y. and Schlott, K. and Brinkmann, R. and Roider, J.},
       title = {Comprehensive detection, grading, and growth behavior evaluation of subthreshold and low intensity photocoagulation lesions by optical coherence tomographic and infrared image analysis},
       journal = {Biomed Res Int},
       volume = {2014},
       pages = {492679},
       note = {2314-6141
    Koinzer, Stefan
    Caliebe, Amke
    Portz, Lea
    Saeger, Mark
    Miura, Yoko
    Schlott, Kerstin
    Brinkmann, Ralf
    Roider, Johann
    Journal Article
    Research Support, Non-U.S. Gov't
    United States
    Biomed Res Int. 2014;2014:492679. doi: 10.1155/2014/492679. Epub 2014 May 12.},
       abstract = {PURPOSE: To correlate the long-term clinical effect of photocoagulation lesions after 6 months, as measured by their retinal damage size, to exposure parameters. We used optical coherence tomographic (OCT)-based lesion classes in order to detect and assess clinically invisible and mild lesions. METHODS: In this prospective study, 488 photocoagulation lesions were imaged in 20 patients. We varied irradiation diameters (100/300 microm), exposure-times (20-200 ms), and power. Intensities were classified in OCT images after one hour, and we evaluated OCT and infrared (IR) images over six months after exposure. RESULTS: For six consecutive OCT-based lesion classes, the following parameters increased with the class: ophthalmoscopic, OCT and IR visibility rate, fundus and OCT diameter, and IR area, but not irradiation power. OCT diameters correlated with exposure-time, irradiation diameter, and OCT class. OCT classes discriminated the largest bandwidth of OCT diameters. CONCLUSION: OCT classes represent objective and valid endpoints of photocoagulation intensity even for "subthreshold" intensities. They are suitable to calculate the treated retinal area. As the area is critical for treatment efficacy, OCT classes are useful to define treatment intensity, calculate necessary lesion numbers, and universally categorize lesions in clinical studies.},
       DOI = {10.1155/2014/492679},
       url = {http://dx.doi.org/10.1155/2014/492679},
       year = {2014},
       type = {Journal Article}
    }
    
  • Li, Z. and Roussakis, E. and Koolen, P. G. and Ibrahim, A. M. and Kim, K. and Rose, L. F. and Wu, J. and Nichols, A. J. and Baek, Y. and Birngruber, R. and Apiou-Sbirlea, G. and Matyal, R. and Huang, T. and Chan, R. and Lin, S. J. and Evans, C. L.: Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage. Biomed Opt Express, no. 5, pp. 3748-64, 2014
    BibTeX Link
    @article{Li2014,
       author = {Li, Z. and Roussakis, E. and Koolen, P. G. and Ibrahim, A. M. and Kim, K. and Rose, L. F. and Wu, J. and Nichols, A. J. and Baek, Y. and Birngruber, R. and Apiou-Sbirlea, G. and Matyal, R. and Huang, T. and Chan, R. and Lin, S. J. and Evans, C. L.},
       title = {Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage},
       journal = {Biomed Opt Express},
       volume = {5},
       number = {11},
       pages = {3748-64},
       note = {2156-7085
    Li, Zongxi
    Roussakis, Emmanuel
    Koolen, Pieter G L
    Ibrahim, Ahmed M S
    Kim, Kuylhee
    Rose, Lloyd F
    Wu, Jesse
    Nichols, Alexander J
    Baek, Yunjung
    Birngruber, Reginald
    Apiou-Sbirlea, Gabriela
    Matyal, Robina
    Huang, Thomas
    Chan, Rodney
    Lin, Samuel J
    Evans, Conor L
    Journal Article
    United States
    Biomed Opt Express. 2014 Oct 1;5(11):3748-64. doi: 10.1364/BOE.5.003748. eCollection 2014 Nov 1.},
       abstract = {Oxygen plays an important role in wound healing, as it is essential to biological functions such as cell proliferation, immune responses and collagen synthesis. Poor oxygenation is directly associated with the development of chronic ischemic wounds, which affect more than 6 million people each year in the United States alone at an estimated cost of $25 billion. Knowledge of oxygenation status is also important in the management of burns and skin grafts, as well as in a wide range of skin conditions. Despite the importance of the clinical determination of tissue oxygenation, there is a lack of rapid, user-friendly and quantitative diagnostic tools that allow for non-disruptive, continuous monitoring of oxygen content across large areas of skin and wounds to guide care and therapeutic decisions. In this work, we describe a sensitive, colorimetric, oxygen-sensing paint-on bandage for two-dimensional mapping of tissue oxygenation in skin, burns, and skin grafts. By embedding both an oxygen-sensing porphyrin-dendrimer phosphor and a reference dye in a liquid bandage matrix, we have created a liquid bandage that can be painted onto the skin surface and dries into a thin film that adheres tightly to the skin or wound topology. When captured by a camera-based imaging device, the oxygen-dependent phosphorescence emission of the bandage can be used to quantify and map both the pO2 and oxygen consumption of the underlying tissue. In this proof-of-principle study, we first demonstrate our system on a rat ischemic limb model to show its capabilities in sensing tissue ischemia. It is then tested on both ex vivo and in vivo porcine burn models to monitor the progression of burn injuries. Lastly, the bandage is applied to an in vivo porcine graft model for monitoring the integration of full- and partial-thickness skin grafts.},
       keywords = {(160.2540) Fluorescent and luminescent materials
    (170.2655) Functional monitoring and imaging
    (170.3880) Medical and biological imaging
    (170.6510) Spectroscopy, tissue diagnostics},
       ISSN = {2156-7085},
       DOI = {10.1364/boe.5.003748},
       year = {2014},
       type = {Journal Article}
    }
    
  • Yoko, Miura: Retinal Laser Therapy: Recent Unique Developments Behind the Brilliant Front Stage of Anti-VEGF Treatment. J Clin Exp Ophthalmol, no. 5, pp. 1-2, 2014
    BibTeX Link
    @article{Miura2014,
       author = {Yoko, Miura},
       title = {Retinal Laser Therapy: Recent Unique Developments Behind the Brilliant Front Stage of Anti-VEGF Treatment},
       journal = {J Clin Exp Ophthalmol},
       volume = {5},
       number = {6},
       pages = {1-2},
       note = {-},
       DOI = {http://dx.doi.org/10.4172/2155-9570.1000e114},
       year = {2014},
       type = {Journal Article}
    }
    
    
  • Kolb, Jan Philip and Klein, Thomas and Mohler, Kathrin and Wieser, Wolfgang and Reznicek, Lukas and Kernt, Marcus and Kampik, Anselm and Neubauer, Aljoscha S. and Huber, Robert: Widefield Megahertz (MHz) OCT of diabetic retinopathy. Investigative Ophthalmology & Visual Science, no. 55, pp. 5018-5018, 2014
    BibTeX Link
    @article{Kolb2014,
       author = {Kolb, Jan Philip and Klein, Thomas and Mohler, Kathrin and Wieser, Wolfgang and Reznicek, Lukas and Kernt, Marcus and Kampik, Anselm and Neubauer, Aljoscha S. and Huber, Robert},
       title = {Widefield Megahertz (MHz) OCT of diabetic retinopathy},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {55},
       number = {13},
       pages = {5018-5018},
       abstract = { PurposeTo investigate morphological appearance of diabetic retinopathy and laser effects in panretinal laser photocoagulation with a widefield MHz OCT (60° field of view, 120° center angle).  MethodsA custom swept-source OCT device with a 1060nm Fourier-domain mode-locked laser source achieving a line rate of 1.68MHz was used to investigate 15 consecutive patients with diabetic retinopathy. Within 1.82s datasets consisting of 2088x1024 A-scans over 60° were acquired. The densely sampled three dimensional datasets were investigated in various B-Scan cross-sections as well as en-face images and 3D reconstructions. Selected findings were compared with SLO images from a widefield SLO (Optos, Dunfermeline, UK).  ResultsOn the densely sampled 3D and en-face images, hard exsudates, peripheral laser spots as well as proliferative neovascularization were readily detected. Characteristic structural changes resulting from laser burns could easily be visualized across the whole field of view in the OCT datasets. In addition, depth-scans and B-scans allowed to identify additional structural changes including IS/OS disruption, RPE detachment or neovascularisation reaching into the vitreous across the field of view.  ConclusionsThree dimensional visualization of the retina over 60° field of view with a MHz OCT is feasible in patients with diabetic retinopathy. It might allow additional clinical insights in peripheral changes such as laser spots or retinal neovascularisation. 3D reconstruction of OCT dataset of patient with diabetic retinopathy with neovascularization (green), hard exsudates (yellow), epiretinal membrane and peripheral retinal scars after panretinal laser treatment (blue). Indications with arrows are examplatory. Top: En-face of same patient as in figure 1. Red line indicates the position of B-Frame below. Retinal scars due to focal laser coagulation temporal of the fovea visible. Bottom: Neovascularisation (green) reaching into the vitreous is visible on the left side. Retinal scars especially on RPE level due to panretinal laser treatment (blue) are observable.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2014},
       type = {Journal Article}
    }
    
  • Kortuem, Karsten Ulrich and Kolb, Jan Philip and Klein, Thomas and Mohler, Kathrin and Kampik, Anselm and Neubauer, Aljoscha S. and Wieser, Wolfgang and Huber, Robert and Haritoglou, Christos: Wide Field MHz OCT imaging of the vitreoretinal interface. Investigative Ophthalmology & Visual Science, no. 55, pp. 1619-1619, 2014
    BibTeX Link
    @article{Kortuem2014,
       author = {Kortuem, Karsten Ulrich and Kolb, Jan Philip and Klein, Thomas and Mohler, Kathrin and Kampik, Anselm and Neubauer, Aljoscha S. and Wieser, Wolfgang and Huber, Robert and Haritoglou, Christos},
       title = {Wide Field MHz OCT imaging of the vitreoretinal interface},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {55},
       number = {13},
       pages = {1619-1619},
       abstract = { PurposeTo investigate changes caused by vitreoretinal interface disease with a multi-MHz OCT over a wide field of view of 60°.  MethodsWe used custom swept-source OCT device with a 1060nm Fourier-domain mode-locked laser source achieving a line rate of 1.68MHz. Within 1.82s datasets consisting of 2088x1024 A-scans over 60° were acquired from 5 patients with vitreoretinal traction due to VMTS and 3 patients with vascular pathology. The densely sampled three dimensional datasets were investigated in B-Scan cross-sections as well as en-face images and 3D reconstructions.  ResultsVitreoretinal traction could be imaged well in most of the cases, although the current sensitivity of the system limited image quality. Across the whole 60° field of view in the OCT datasets vitreoretinal tractions could be topographically assessed, always including the macula and optic disc, where vitreous anchorage could be shown. By means of the depth-scans as part of the 3D OCT volume we could image traction-associated retinal changes such as retinal elevation or RPE detachment.  ConclusionsThis unique three dimensional visualization of the retina over 60° field of view with a MHz OCT is feasible in patients with vitreoretinal interface disease and might offer additional clinical insights on the three-dimensional topology of tractional changes. Three dimensional Wide Field MHz OCT retinal and vitreous visualization.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2014},
       type = {Journal Article}
    }
    
  • Klein, Thomas and Draxinger, Wolfgang and Mohler, Kathrin and Kolb, Jan Philip and Wieser, Wolfgang and Kampik, Anselm and Neubauer, Aljoscha S. and Wolf, Armin and Huber, Robert: Wide-field choroidal thickness and en-face maps of patients created with MHz-OCT. Investigative Ophthalmology & Visual Science, no. 55, pp. 1620-1620, 2014
    BibTeX Link
    @article{Klein2014,
       author = {Klein, Thomas and Draxinger, Wolfgang and Mohler, Kathrin and Kolb, Jan Philip and Wieser, Wolfgang and Kampik, Anselm and Neubauer, Aljoscha S. and Wolf, Armin and Huber, Robert},
       title = {Wide-field choroidal thickness and en-face maps of patients created with MHz-OCT},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {55},
       number = {13},
       pages = {1620-1620},
       abstract = { PurposeTo study the feasibility of simultaneous peripheral and central choroidal thickness measurement and en-face visualization in patients with a variety of diseases, using a single high-resolution wide-field MHz-OCT dataset spanning more than 50 degrees field of view.  MethodsIn this retrospective study, choroidal morphology of 29 patients imaged with MHz-OCT was assessed. MHz-OCT is a custom SS-OCT operating at 1060nm and an axial line rate of 1.68MHz. The high speed, more than 30 times faster than current commercial devices, enabled a very high resolution scan protocol of 2088x1024 A-scans over a wide field of ~60 degrees. However, due to the very high speed, signal strength is lower compared to slower devices. Hence, thickness and structure maps were only created for selected datasets: The positions of Bruch’s membrane and choroid sclera junction were determined manually by a trained observer a subset of all A-scans, from which thickness and intensity maps were created.  ResultsDespite relative low signal strength, the choroid sclera junction could be clearly observed over the entire unshadowed image area in 15 of 29 patients. Apart from shadowing, visibility of this junction shows strong variation even within a single dataset due to varying retinal thickness, eye-blinks, saccades and retinal curvature. Thus, thickness could be evaluated at least in some areas for all 29 patients, especially in the periphery. Moreover, choroidal thickness varied considerably intra- and interindividually. In two patients, abrupt changes of the choroid were observed in the temporal periphery, which may resemble morphology or imaging artifact. In addition to thickness, en-face choroidal structure maps were extracted from the segmented OCT datasets. Visibility of choroidal vasculature in these maps correlates with choroidal thickness.  ConclusionsChoroidal thickness and structure in patients could be visualized over large areas for the first time. Due to focal choroidal thickness changes with large thickness gradients, high-density scan protocols may be favorable for OCT-based investigations of the choroid. En-face images of the choroid can be extracted from these high-resolution datasets, but the influence of choroidal thickness on the image information should be taken into account. Choroidal en-face image (top), color-coded thickness map with superimposed structural image (middle) and OCT B-frame (bottom) for two eyes (A,B).},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2014},
       type = {Journal Article}
    }
    
  • Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon: Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy. Optics Letters, no. 39, pp. 45-47, 2014
    BibTeX Link Link
    @article{Ansari2014,
       author = {Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon},
       title = {Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy},
       journal = {Optics Letters},
       volume = {39},
       number = {1},
       pages = {45-47},
       abstract = {We present an ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy (SD-OCPM) system that combines submicrometer transverse spatial resolution and subnanometer optical path length sensitivity, with an acquisition speed of over 217,000&#x2009;&#x2009;voxels/s. The proposed SD-OCPM system overcomes two significant drawbacks of traditional common-path interferometers&#x2014;limited transverse spatial resolution and suboptimal detection sensitivity&#x2014;while maintaining phase stability that is comparable with common-path interferometer setups. The transverse and axial spatial resolution of the setup is measured to be 0.6 and 1.9&#xA0;&#x3BC;m, respectively, with a phase sensitivity of 0.0027&#xA0;rad (corresponds to optical path length sensitivity of 110&#xA0;pm). High-speed acquisition allows for phase-sensitive 4D imaging of biological samples with subcellular resolution.},
       keywords = {Microscopy
    Coherence imaging
    Three-dimensional microscopy},
       DOI = {10.1364/OL.39.000045},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-39-1-45},
       year = {2014},
       type = {Journal Article}
    }
    
  • Iwami, H. and Pruessner, J. and Shiraki, K. and Brinkmann, R. and Miura, Y.: Protective effect of a laser-induced sub-lethal temperature rise on RPE cells from oxidative stress. Exp Eye Res, no. 124c, pp. 37-47, 2014
    BibTeX Link
    @article{Iwami2014,
       author = {Iwami, H. and Pruessner, J. and Shiraki, K. and Brinkmann, R. and Miura, Y.},
       title = {Protective effect of a laser-induced sub-lethal temperature rise on RPE cells from oxidative stress},
       journal = {Exp Eye Res},
       volume = {124c},
       pages = {37-47},
       note = {1096-0007
    Iwami, Hisashi
    Pruessner, Joachim
    Shiraki, Kunihiko
    Brinkmann, Ralf
    Miura, Yoko
    Journal article
    Exp Eye Res. 2014 May 5;124C:37-47. doi: 10.1016/j.exer.2014.04.014.},
       abstract = {Recently introduced new technologies that enable temperature-controlled laser irradiation on the RPE allowed us to investigate temperature-resolved RPE cell responses. In this study we aimed primarily to establish an experimental setup that can realize laser irradiation on RPE cell culture with the similar temperature distribution as in the clinical application, with a precise time/temperature history. With this setup, we conducted investigations to elucidate the temperature-dependent RPE cell biochemical responses and the effect of transient hyperthermia on the responses of RPE cells to the secondary-exposed oxidative stress. Porcine RPE cells cultivated in a culture dish (inner diameter = 30 mm) with culture medium were used, on which laser radiation (lambda = 1940 nm, spot diameter = 30 mm) over 10 s was applied as a heat source. The irradiation provides a radially decreasing temperature profile which is close to a Gaussian shape with the highest temperature in the center. Power setting for irradiation was determined such that the peak temperature (Tmax) in the center of the laser spot at the cells reaches from 40 degrees C to 58 degrees C (40, 43, 46, 50, 58 degrees C). Cell viability was investigated with ethidium homodimer III staining at the time points of 3 and 24 h following laser irradiation. Twenty four hours after laser irradiation the cells were exposed to hydrogen peroxide (H2O2) for 5 h, followed by the measurement of intracellular glutathione, intracellular 4-hydroxynonenal (HNE) protein adducts, and secreted vascular endothelial growth factor (VEGF). The mean temperature threshold for RPE cell death after 3 h was found to be around 52 degrees C, and for 24 h around 50 degrees C with the current irradiation setting. A sub-lethal preconditioning on Tmax = 43 degrees C significantly induced the reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, and decreased H2O2-induced increase of intracellular 4-HNE protein adducts. Although sub-lethal hyperthermia (Tmax = 40 degrees C, 43 degrees C, and 46 degrees C) caused a slight increase of VEGF secretion in 6 h directly following irradiation, secondary exposed H2O2-induced VEGF secretion was significantly reduced in the sub-lethally preheated groups, where the largest effect was seen following the irradiation with Tmax = 43 degrees C. In summary, the current results suggest that sub-lethal thermal laser irradiation on the RPE at Tmax = 43 degrees C for 10 s enhances cell defense system against oxidative stress, with increasing the GSH/GSSG ratio. Together with the results that the decreased amount of H2O2-induced 4-HNE in sub-lethally preheated RPE cells was accompanied by the lower secretion of VEGF, it is also strongly suggested that the sub-lethal hyperthermia may modify RPE cell functionality to protect RPE cells from oxidative stress and associated functional decrease, which are considered to play a significant role in the pathogenesis of age-related macular degeneration and other chorioretinal degenerative diseases.},
       ISSN = {0014-4835},
       DOI = {10.1016/j.exer.2014.04.014},
       year = {2014},
       type = {Journal Article}
    }
    
  • Rohde, I. and Masch, J. M. and Theisen-Kunde, D. and Marczynski-Bühlow, M. and Bombien Quaden, R. and Lutter, G. and Brinkmann, R.: Resection of Calcified Aortic Heart Leaflets In Vitro by Q-Switched 2 µm Microsecond Laser Radiation. Journal of Cardiac Surgery, no. 30, pp. 157-162, 2014
    BibTeX Link
    @article{Rohde2014,
       author = {Rohde, I. and Masch, J. M. and Theisen-Kunde, D. and Marczynski-Bühlow, M. and Bombien Quaden, R. and Lutter, G. and Brinkmann, R.},
       title = {Resection of Calcified Aortic Heart Leaflets In Vitro by Q-Switched 2 µm Microsecond Laser Radiation},
       journal = {Journal of Cardiac Surgery},
       volume = {30},
       number = {2},
       pages = {157-162},
       ISSN = {1540-8191},
       DOI = {10.1111/jocs.12481},
       url = {http://dx.doi.org/10.1111/jocs.12481},
       year = {2014},
       type = {Journal Article}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. ATu3P.4 1-3, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Eibl_a,
    abstract = {A fast all fiber based setup for stimulated Raman spectroscopy with a rapidly wavelength swept cw-laser is presented. It enables flexible acquisition of broadband (750 cm-1 to 3150 cm-1) spectra with high resolution (0.5 cm-1).},
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_AT.2014.ATu3P.4},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,stimulated Raman,tunable},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_AT-2014-ATu3P.4},
    year = {2014}
    }
  • Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert: High definition live 3D-OCT in vivo: design and evaluation of a 4D OCT engine with 1 GVoxel/s. Biomedical optics express, no. 5, pp. 2963-77, sep,, 2014
    BibTeX Link
    @Article{HU_2014_Wieser_a,
      Title                    = {{High definition live 3D-OCT in vivo: design and evaluation of a 4D OCT engine with 1 GVoxel/s}},
      Author                   = {Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2014},
    
      Month                    = sep,
      Number                   = {9},
      Pages                    = {2963--77},
      Volume                   = {5},
    
      Doi                      = {10.1364/BOE.5.002963},
      ISSN                     = {2156-7085},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-9-2963}
    }
  • Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert: Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. 1-2, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Karpf_b,
    abstract = {A hyperspectral stimulated Raman microscopy system using rapidly wavelength swept lasers is presented. Imaging of biological samples with shot noise limited detection is demonstrated with the fiber based setup.},
    author = {Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_SI.2014.SM3P.3},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman microscopy,Scattering,stimulated Raman,tunable},
    pages = {1--2},
    publisher = {OSA},
    title = {{Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_SI-2014-SM3P.3},
    year = {2014}
    }
  • Reznicek, Lukas and Klein, Thomas and Wieser, Wolfgang and Kernt, Marcus and Wolf, Armin and Haritoglou, Christos and Kampik, Anselm and Huber, Robert and Neubauer, Aljoscha S: Megahertz ultra-wide-field swept-source retina optical coherence tomography compared to current existing imaging devices.. Graefe`s archive for clinical and experimental ophthalmology Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie, no. 252, pp. 1009-16, jun,, 2014
    BibTeX Link
    @Article{HU_2014_Reznicek_a,
      Title                    = {{Megahertz ultra-wide-field swept-source retina optical coherence tomography compared to current existing imaging devices.}},
      Author                   = {Reznicek, Lukas and Klein, Thomas and Wieser, Wolfgang and Kernt, Marcus and Wolf, Armin and Haritoglou, Christos and Kampik, Anselm and Huber, Robert and Neubauer, Aljoscha S},
      Journal                  = {Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv f\"{u}r klinische und experimentelle Ophthalmologie},
      Year                     = {2014},
    
      Month                    = jun,
      Number                   = {6},
      Pages                    = {1009--16},
      Volume                   = {252},
    
      Doi                      = {10.1007/s00417-014-2640-4},
      ISSN                     = {1435-702X},
      Url                      = {http://link.springer.com/article/10.1007\%2Fs00417-014-2640-4}
    }
  • Zhang, Yaokun and Pfeiffer, Tom and Weller, Marcel and Wieser, Wolfgang and Huber, Robert and Raczkowsky, Jörg and Schipper, Jörg and Wörn, Heinz and Klenzner, Thomas: Optical coherence tomography guided laser cochleostomy: towards the accuracy on tens of micrometer scale. BioMed research international, no. 2014, pp. 251814-24, jan,, 2014
    BibTeX Link
    @Article{HU_2014_Zhang_a,
      Title                    = {{Optical coherence tomography guided laser cochleostomy: towards the accuracy on tens of micrometer scale}},
      Author                   = {Zhang, Yaokun and Pfeiffer, Tom and Weller, Marcel and Wieser, Wolfgang and Huber, Robert and Raczkowsky, J\"{o}rg and Schipper, J\"{o}rg and W\"{o}rn, Heinz and Klenzner, Thomas},
      Journal                  = {BioMed research international},
      Year                     = {2014},
      Month                    = jan,
      Pages                    = {251814--24},
      Volume                   = {2014},
      Doi                      = {10.1155/2014/251814},
      ISSN                     = {2314-6141},
      Url                      = {http://www.hindawi.com/journals/bmri/2014/251814/}
    }

2013

  • Trost, Andrea and Schroedl, Falk and Strohmaier, Clemens A and Bogner, Barbara and Runge, Christian and Kaser-Eichberger, Alexandra and Krefft, Karolina Anna and Vogel, Alfred and Linz, Norbert and Freidank, Sebastian and Hilpert, Andrea and Zimmermann, Inge and Grabner, Günther and Reitsamer, Herbert A: A new nanosecond UV-laser at 355 nm: early results of corneal flap cutting in a rabbit model. Investigative Ophthalmology & Visual Science, 2013
    BibTeX Link
    @article{Trost2013,
       author = {Trost, Andrea and Schroedl, Falk and Strohmaier, Clemens A and Bogner, Barbara and Runge, Christian and Kaser-Eichberger, Alexandra and Krefft, Karolina Anna and Vogel, Alfred and Linz, Norbert and Freidank, Sebastian and Hilpert, Andrea and Zimmermann, Inge and Grabner, Günther and Reitsamer, Herbert A},
       title = {A new nanosecond UV-laser at 355 nm: early results of corneal flap cutting in a rabbit model},
       journal = {Investigative Ophthalmology & Visual Science},
       abstract = {Purpose: A new 355nm UV laser was used for corneal flap cutting in an animal model and tested for clinical and morphological alterations. Methods: Corneal flaps were created (Chinchilla Bastards; n=25) with an UV-nanosecond laser at 355nm (150kHz, pulse duration 850ps, spot-size 1µm, spot-spacing 6x6µm, side-cut Δz 1µm; cutting depth 130µm) and pulse energies of 2.2 or 2.5µJ, respectively. Following slit lamp examination, animals were sacrificed at 6, 12 and 24hrs after treatment. Corneas were prepared for histology (HE, TUNEL-assay) and evaluated statistically, followed by ultrastructural investigations. Results: Laser treatment was tolerated well, flap lift was easier at 2.5µJ compared to 2.2µJ. Standard HE at 24hrs revealed intact epithelium in the horizontal cut, with similar increase in corneal thickness at both energies. Irrespective of energy levels, TUNEL assay revealed comparable numbers of apoptotic cells in the horizontal and vertical cut at 6/12/24hrs, becoming detectable in the horizontal cut as an acellular stromal band at 24hrs. Ultrastructural analysis revealed regular morphology in the epi- and endothelium, while in the stroma, disorganized collagen lamellae were detectable representing the horizontal cut, again irrespective of energy levels applied. Conclusions:This new UV-laser revealed no epithelial nor endothelial damage at energies feasible for corneal flap cutting. Observed corneal swelling was lower compared to existing UV-lasers-studies, albeit total energy applied here was much higher. Observed loss of stromal keratinocytes is comparable to available laser systems. Therefore, this new laser is suitable for refractive surgery, awaiting its test in a chronic environment.},
       DOI = {10.1167/iovs.13-12580},
       url = {http://www.iovs.org/content/early/2013/10/28/iovs.13-12580.abstract},
       year = {2013},
       type = {Journal Article}
    }
    
  • Koop, N: Schreib‘ mal wieder! Neue Laser-Markierungsverfahren und spezielle Mikrobearbeitungen. GIT Labor-Fachzeitschrift, 2013
    BibTeX
    @article{Koop2013,
       author = {Koop, N},
       title = {Schreib‘ mal wieder! Neue Laser-Markierungsverfahren und spezielle Mikrobearbeitungen},
       journal = {GIT Labor-Fachzeitschrift},
       number = {9},
       year = {2013},
       type = {Journal Article}
    }
    
  • Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R: Two-photon Microscopy and Fluorescence Lifetime Analysis of Lipid Peroxidation Product in Photoreceptor Outer Segment and in Retinal Pigment Epithelial Cell. ARVO Meeting Abstracts, March 26, 2012, 2013
    BibTeX Link
    @misc{Miura2013,
       author = {Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R },
       title = {Two-photon Microscopy and Fluorescence Lifetime Analysis of Lipid Peroxidation Product in Photoreceptor Outer Segment and in Retinal Pigment Epithelial Cell},
       publisher = {ARVO Meeting Abstracts},
       month = {March 26, 2012 },
       url = {http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=57630548-893d-4e45-9ddc-b6f547dd4ff0&cKey=d08a30bc-fe98-40a2-8a1c-1b171e4becd3&mKey=f0fce029-9bf8-4e7c-b48e-9ff7711d4a0e},
       year = {2013},
       type = {Poster}
    }
    
  • Lee, Hee Yoon and Sudkamp, Helge and Marvdashti, Tahereh and Ellerbee, Audrey K.: Interleaved optical coherence tomography. Optics Express, no. 21, pp. 26542-26556, 2013
    BibTeX Link
    @article{Lee2013,
       author = {Lee, Hee Yoon and Sudkamp, Helge and Marvdashti, Tahereh and Ellerbee, Audrey K.},
       title = {Interleaved optical coherence tomography},
       journal = {Optics Express},
       volume = {21},
       number = {22},
       pages = {26542-26556},
       abstract = {We present a novel and cost-effective technique &#x2013; interleaved optical coherence tomography (iOCT) &#x2013; to enhance the imaging speed of swept source OCT systems by acquiring data from multiple lateral positions simultaneously during a single wavelength sweep, using a single detector and a virtually imaged phase array (VIPA) as a multi-band demultiplexer. This technique uses spectral encoding to convert coherence length into higher imaging speed; the speed enhancement factor is independent of the source speed or center wavelength, and the effective A-scan rate scales linearly with sweep speed. The optical configuration requires only a change in the sample arm of a traditional OCT system and preserves the axial resolution and fall-off characteristic of a traditional SS-OCT using the same light source. Using 10kHz, 20kHz and 100kHz sources we provide a first demonstration of image speed enhancement factors of up to 12, 6 and 10, respectively, which yield effective A-scan rates of 120kHz, 120kHz and 1MHz for B-scan imaging, with a sensitivity of up to 82.5 dB. We also show that iOCT can image faster dynamics than traditional OCT B-scan imaging and is capable of 3D biological imaging. The iOCT concept suggests a new route to high-speed OCT imaging for laser developers: that is, by focusing on improving the coherence length and linewidth of existing and emerging sources. Hence, iOCT is a nice complement to ongoing research and commercial efforts to enable faster imaging through development of lasers with faster sweep rates, and offers new hope for existing sources with slow sweep rates and potential for enhancement of coherence length to compete with faster sources to achieve high-speed OCT.},
       keywords = {Optical design of instruments
    Imaging systems
    Coherence imaging
    Optical coherence tomography
    Optical diagnostics for medicine},
       DOI = {10.1364/OE.21.026542},
       url = {http://www.opticsexpress.org/abstract.cfm?URI=oe-21-22-26542},
       year = {2013},
       type = {Journal Article}
    }
    
  • Cordes, Jens and Nguyen, Felix and Lange, Birgit and Brinkmann, Ralf and Jocham, Dieter: Damage of Stone Baskets by Endourologic Lithotripters: A Laboratory Study of 5 Lithotripters and 4 Basket Types. Advances in Urology, no. 2013, pp. 6, 2013
    BibTeX Link
    @article{Cordes2013,
       author = {Cordes, Jens and Nguyen, Felix and Lange, Birgit and Brinkmann, Ralf and Jocham, Dieter},
       title = {Damage of Stone Baskets by Endourologic Lithotripters: A Laboratory Study of 5 Lithotripters and 4 Basket Types},
       journal = {Advances in Urology},
       volume = {2013},
       pages = {6},
       DOI = {10.1155/2013/632790},
       url = {http://dx.doi.org/10.1155/2013/632790},
       year = {2013},
       type = {Journal Article}
    }
    
  • Brinkmann, Ralf and Iwami, Hisashi and Pruessner, Joachim and Danicke, Veit and Miura, Yoko: Temperature-dependent response of retinal pigment epithelial cells to laser irradiation. Invest. Ophthalmol. Vis. Sci., no. 54, pp. 1809-, 2013
    BibTeX Link
    @article{Brinkmann2013,
       author = {Brinkmann, Ralf and Iwami, Hisashi and Pruessner, Joachim and Danicke, Veit and Miura, Yoko},
       title = {Temperature-dependent response of retinal pigment epithelial cells to laser irradiation},
       journal = {Invest. Ophthalmol. Vis. Sci.},
       volume = {54},
       number = {6},
       pages = {1809-},
       abstract = {PurposeSublethal thermal therapy of the retinal pigment epithelium (RPE) is discussed as a new prophylactic therapy for age-related macular degeneration. However, temperature-dependent RPE cell effects have not been well elucidated. We investigated the biochemical responses of RPE cells following sublethal to lethal thermal laser irradiation. MethodsPorcine RPE cells cultured in a dish (33mm) were heated with a Thulium laser (1.92{micro}m, 1-20W, 10s) over a spot of 3mm. Temperatures during irradiation were measured with thermocouples. Cell viability was examined using annexin-V, ethidium homodimer III and Hoechst 33342 for detecting apoptotic, necrotic and living cell, respectively, by using fluorescence microscopy for localization and flow cytometry for quantification. Secretion of vascular endothelial growth factor (VEGF) for 6h following irradiation on different temperatures was assessed with Elisa assay. In order to examine a protective effect of sublethal hyperthremia, the cells were heated up to 45C 24h prior to the exposure of 2 mM hydroxyl peroxide (H2O2) for 5 h. The involvement of TRPV (Transient Receptor Potential Vanilloid)-1 receptor, which is activated with temperatures > 43C, was investigated by adding capsazepin, a TRPV-1 inhibitor, before irradiation. ResultsCell apoptosis and necrosis was observed 24 h after irradiation with a central peak temperature [&ge;]52C. Fluorescence microscopy revealed apoptotic cells around the central necrotic area. VEGF secretion for 6h after irradiation was significantly increased at peak temperatures between 40 and 52C in a temperature dependent manner (max. 110%, p<0.05), whereas the total secretion decreases with temperatures > 52C. Pre-irradiation onto 45C significantly reduced H2O2-induced cell death after 5h compared to non-heated cells (total cell death: 15.6% to 10.2%, necrosis: 6% to 4 %, early apoptosis: 5.1% to 3.6%; p<0.01). These effects were not observed in the existence of capsazepin during laser irradiation. ConclusionsThe number of apoptotic and necrotic RPE cells increase at least over 24h following thermal laser irradiation. Sublethal temperatures between 40 and 52C seem to induce various cellular responses as VEGF secretion, which might be related to the protective effect against oxidative stress. Results with capsazepin suggest that TRPV-1 channel activation by hyperthermia is essential to exert this protective effect.},
       url = {http://abstracts.iovs.org/cgi/content/abstract/54/6/1809},
       year = {2013},
       type = {Journal Article}
    }
    
  • Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon: Stray light rejection by structured illumination. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Peer2013,
       author = {Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon},
       title = {Stray light rejection by structured illumination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Bliedtner, Kathrin and Seifert, Eric and Brinkmann, Ralf: Temperature induced tissue deformation monitored by dynamic speckle interferometry. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Bliedtner2013,
       author = {Bliedtner, Kathrin and Seifert, Eric and Brinkmann, Ralf},
       title = {Temperature induced tissue deformation monitored
    by dynamic speckle interferometry},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon: Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Fleischhauer2013,
       author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon},
       title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Horstmann, Jens and Brinkmann, Ralf: Non-contact photoacoustic tomography using holographic full field detection. no. 8800, pp. 880007-880007-6, Proc. SPIE,
    BibTeX Link
    @inproceedings{Horstmann2013,
       author = {Horstmann, Jens and Brinkmann, Ralf},
       title = {Non-contact photoacoustic tomography using holographic full field detection},
       publisher = {Proc. SPIE},
       volume = {8800},
       pages = {880007-880007-6},
       note = {10.1117/12.2033599},
       abstract = {An innovative very fast non-contact imaging technique for Photoacoustic Tomography is introduced. It is based on holographic optical speckle detection of a transiently altering surface topography for the reconstruction of absorbing targets. The surface movement is obtained by parallel recording of speckle phase changes known as Electronic Speckle Pattern Interferometry. Due to parallelized 2-D camera detection and repetitive excitation with variable delay with respect to the image acquisition, data recording of whole volumes for Photoacoustic Imaging can be completed in times far below one second. The size of the detected area is scalable by optical magnification. As a proof of concept, an interferometric setup is realized, capable of surface displacement detection with an axial resolution of less than 3 nm. The potential of the proposed method for in vivo Photoacoustic Imaging is discussed.},
       url = {http://dx.doi.org/10.1117/12.2033599},
       type = {Conference Proceedings}
    }
    
  • Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon: Speckle variance optical coherence tomography for imaging microcirculation. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Klawitter2013,
       author = {Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon},
       title = {Speckle variance optical coherence tomography for
    imaging microcirculation},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Rohde, Ingo and Masch, Jennifer- M. and Theisen-Kunde, Dirk and Marczynski-Bühlow, Martin and Lutter, Georg and Brinkmann, Ralf: Cardiovascular damage after cw and Q-switched 2μm laser irradiation. no. 8803, pp. 88030I-88030I-6,
    BibTeX Link
    @inproceedings{Rohde2013,
       author = {Rohde, Ingo and Masch, Jennifer- M. and Theisen-Kunde, Dirk and Marczynski-Bühlow, Martin and Lutter, Georg and Brinkmann, Ralf},
       title = {Cardiovascular damage after cw and Q-switched 2μm laser irradiation},
       volume = {8803},
       pages = {88030I-88030I-6},
       note = {10.1117/12.2033550},
       abstract = {Aiming for laser-assisted resection of calcified aortic valve structures for Transcatheter Aortic Valve Implantation (TAVI), a Q-switched Tm:YAG laser emitting at a wavelength of 2.01 μm was used to evaluate the cutting efficiency on highly calcified human aortic leaflets in-vitro. The calcified aortic leaflets were examined regarding ablation rates and debris generation, using a pulse energy of 4.3 mJ, a pulse duration of 0.8-1 μs and a repetition rate of 1 kHz. The radiation was transmitted via a 200 μm core diameter quartz fiber. Resection was performed in a fiber-tissue contact mode on water-covered samples in a dish. The remnant particles were analyzed with respect to quantity and size by light microscopy. Additionally, soft tissue of porcine aortic vessels was examined for histologically detectable thermo-mechanical damage after continuous wave and Q-switched 2μm laser irradiation. An ablation rate of 36.7 ± 25.3 mg/min could be realised on highly calcified aortic leaflets, with 85.4% of the remnant particles being &lt;6 μm in diameter. The maximum damaged area of the soft tissue was &lt; 1 mm for both, cw and pulsed laser irradiation. This limits the expected collateral damage of healthy tissue during the medical procedure. Overall, the Q-switched Tm:YAG laser system showed promising results in cutting calcified aortic valves, transmitting sufficient energy through a small flexible fibre.},
       url = {http://dx.doi.org/10.1117/12.2033550},
       type = {Conference Proceedings}
    }
    
  • Theisen-Kunde, D. and Wolken, H. and Ellebrecht, D. and Danicke, V. and Wurster, L. and Kleemann, M. and Birngruber, R.: Investigation of water spray to reduce collateral thermal damage during laser resection of soft tissue. no. 8803, pp. 88030F-88030F-4,
    BibTeX Link
    @inproceedings{Theisen-Kunde2013,
       author = {Theisen-Kunde, D. and Wolken, H. and Ellebrecht, D. and Danicke, V. and Wurster, L. and Kleemann, M. and Birngruber, R.},
       title = {Investigation of water spray to reduce collateral thermal damage during laser resection of soft tissue},
       volume = {8803},
       pages = {88030F-88030F-4},
       note = {10.1117/12.2032384},
       abstract = {To reduce unwanted collateral thermal damage to surrounding tissue and organs during laparoscopic laser dissection (cw, wavelength: 1.9μm) of porcine liver water spray was used. Size and amount of the produced water droplets of the water spray were photographed by short time imaging and analyzed by imaging software. At in vivo measurements on fresh porcine liver the depth of thermal damage was reduced by 85 % with water spray and the lateral size of thermal damage at the tissue surface could be reduced by 67%. This results show that especially for laparoscopic laser surgery water spray application might be a useful tool to avoid unwanted collateral thermal damage.},
       url = {http://dx.doi.org/10.1117/12.2032384},
       type = {Conference Proceedings}
    }
    
  • Wurster, Lara M and Warger, W C and Gora, M J and Carruth, R and Tearny, G J and Birngruber, Reginald: Design and development of a miniaturized scanning probe. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Wurster2013,
       author = {Wurster, Lara M and Warger, W C and Gora, M J and Carruth, R and Tearny, G J and Birngruber, Reginald},
       title = {Design and development of a miniaturized scanning probe},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Evers, Michael and Welford, David and Mansteine, Dieter and Birngruber, Reginald: Mid-infrared laser spectroscopy using a tunable gain-switched Cr2+: ZnSe laser. in Studierendentagung, Unversität zu Lübeck,
    BibTeX
    @inproceedings{Ever2013,
       author = {Evers, Michael and Welford, David and Mansteine, Dieter and Birngruber, Reginald},
       title = {Mid-infrared laser spectroscopy using a tunable gain-switched Cr2+: ZnSe laser},
       booktitle = {Studierendentagung},
       publisher = {Unversität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K: An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Sudkamp2013,
       author = {Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K},
       title = {An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    
  • Seifert, Eric and Roh, Young-Jung and Fritz, Andreas and Park, Young Gun and Kang, Seungbum and Theisen-Kunde, Dirk and Brinkmann, Ralf: Automatic irradiation control by an optical feedback technique for selective retina treatment (SRT) in a rabbit model. no. 8803, pp. 880303-880303-6,
    BibTeX Link
    @inproceedings{Seifert2013,
       author = {Seifert, Eric and Roh, Young-Jung and Fritz, Andreas and Park, Young Gun and Kang, Seungbum and Theisen-Kunde, Dirk and Brinkmann, Ralf},
       title = {Automatic irradiation control by an optical feedback technique for selective retina treatment (SRT) in a rabbit model},
       volume = {8803},
       pages = {880303-880303-6},
       note = {10.1117/12.2033560},
       abstract = {Selective Retina Therapy (SRT) targets the Retinal Pigment Epithelium (RPE) without effecting neighboring layers as the photoreceptors or the choroid. SRT related RPE defects are ophthalmoscopically invisible. Owing to this invisibility and the variation of the threshold radiant exposure for RPE damage the treating physician does not know whether the treatment was successful or not. Thus measurement techniques enabling a correct dosing are a demanded element in SRT devices. The acquired signal can be used for monitoring or automatic irradiation control. Existing monitoring techniques are based on the detection of micro-bubbles. These bubbles are the origin of RPE cell damage for pulse durations in the ns and μs time regime 5μs. The detection can be performed by optical or acoustical approaches. Monitoring based on an acoustical approach has already been used to study the beneficial effects of SRT on diabetic macula edema and central serous retinopathy. We have developed a first real time feedback technique able to detect micro-bubble induced characteristics in the backscattered laser light fast enough to cease the laser irradiation within a burst. Therefore the laser energy within a burst of at most 30 pulses is increased linearly with every pulse. The laser irradiation is ceased as soon as micro-bubbles are detected. With this automatic approach it was possible to observe invisible lesions, an intact photoreceptor layer and a reconstruction of the RPE within one week.},
       url = {http://dx.doi.org/10.1117/12.2033560},
       type = {Conference Proceedings}
    }
    
  • Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon: Towards microscopic resolution in holoscopy. pp. 85711O-85711O, 2013
    BibTeX Link Link
    @article{Franke2013,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon},
       title = {Towards microscopic resolution in holoscopy},
       pages = {85711O-85711O},
       note = {10.1117/12.2006806},
       abstract = {Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.},
       DOI = {10.1117/12.2006806},
       url = {http://dx.doi.org/10.1117/12.2006806},
       year = {2013},
       type = {Journal Article}
    }
    
  • Baade, Alexander and Schlott, Kerstin and Birngruber, Reginald and Brinkmann, Ralf: A numerical model for heat and pressure propagation for temperature controlled retinal photocoagulation. no. 8803, pp. 88030O-88030O-9,
    BibTeX Link
    @inproceedings{Baade2013,
       author = {Baade, Alexander and Schlott, Kerstin and Birngruber, Reginald and Brinkmann, Ralf},
       title = {A numerical model for heat and pressure propagation for temperature controlled retinal photocoagulation},
       volume = {8803},
       pages = {88030O-88030O-9},
       note = {10.1117/12.2033590},
       abstract = {Retinal photocoagulation is an established treatment for various retinal diseases. The temperature development during a treatment can be monitored by applying short laser pulses in addition to the treatment laser light. The laser pulses induce thermoelastic pressure waves that can be detected at the cornea. We present a numerical model to examine the temperature development during the treatment as well as the formation and propagation of the ultrasonic waves. Using the model, it is possible to determine the peak temperature during retinal photocoagulation from the measured signal, and investigate the behaviour of the temperature profile and the accuracy of the temperature determination under varying conditions such as inhomogeneous pigmentation or change in irradiation parameters. It was shown that there is an uncertainty of 2.5 -9% in the determination of the peak temperature when the absorption coefficient between the absorbing layers is varied by a factor of 2. Furthermore the model was extended in order to incorporate the photoacoustic pressure generation and wave propagation. It was shown that for an irradiation pulse duration of 75 ns the resulting pressure wave energy is attenuated by 76 % due to frequency dependent attenuation in water.},
       url = {http://dx.doi.org/10.1117/12.2033590},
       type = {Conference Proceedings}
    }
    
  • Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon: Off-axis full-field swept-source optical coherence tomography using holographic refocusing. pp. 857104-857104, 2013
    BibTeX Link Link
    @article{Hillmann2013,
       author = {Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon},
       title = {Off-axis full-field swept-source optical coherence tomography using holographic refocusing},
       pages = {857104-857104},
       note = {10.1117/12.2006436},
       abstract = {We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.},
       DOI = {10.1117/12.2006436},
       url = {http://dx.doi.org/10.1117/12.2006436},
       year = {2013},
       type = {Journal Article}
    }
    
  • Koinzer, Stefan and Saeger, Mark and Hesse, Carola and Portz, Lea and Kleemann, Susanne and Schlott, Kerstin and Brinkmann, Ralf and Roider, Johann: Correlation with OCT and histology of photocoagulation lesions in patients and rabbits. Acta Ophthalmologica, pp. no-no, 2013
    BibTeX Link
    @article{Koinzer2013,
       author = {Koinzer, Stefan and Saeger, Mark and Hesse, Carola and Portz, Lea and Kleemann, Susanne and Schlott, Kerstin and Brinkmann, Ralf and Roider, Johann},
       title = {Correlation with OCT and histology of photocoagulation lesions in patients and rabbits},
       journal = {Acta Ophthalmologica},
       pages = {no-no},
       abstract = {Purpose:  To examine spectral domain optical coherence tomographic (OCT) and histological images from comparable retinal photocoagulation lesions in rabbits, and to correlate these images with comparable OCT images from patients. Methods:  508 rabbit lesions were examined by HE-stained paraffin histology. 1019 rabbit lesions versus 236 patient lesions were examined by OCT, all at the time-points 1 hr, 1 week and 4 weeks after photocoagulation. We analysed 100 μm lesions (in humans) and 133 μm lesions (in rabbits) of 200 ms exposures at powers titrated from the histological threshold up to intense damage. Lesions were matched according to morphological criteria. Results:  Dome-shaped layer alterations, retinal infiltration by round, pigmented cells, outer nuclear layer interruption, and eventually full thickness retinal coagulation are detectable in histology and OCT. Horizontal damage extensions are found 1½ times larger in OCT. More intense irradiation was necessary to induce comparable layer affection in rabbit OCT as in histology. Restoration of the inner retinal layers is only shown in the OCT images. Comparable primary lesions caused more pronounced OCT changes in patients than in rabbits during healing. Conclusions:  Optical coherence tomographic images indicate different tissue changes than histologic images. After photocoagulation, they show wider horizontal damage diameters, but underestimate axial damage particularly during healing. Conclusions on retinal restoration should not be drawn from OCT findings alone. Retinal recovery after comparable initial lesions appears to be more complete in rabbit than in patient OCTs.},
       keywords = {histology
    laser
    optical coherence tomography
    photocoagulation
    retina
    retinal healing},
       year = {2013}
    }
    
  • Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.: Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress. Invest Ophthalmol Vis Sci, 2013
    BibTeX Link
    @article{Miura2013,
       author = {Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.},
       title = {Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress},
       journal = {Invest Ophthalmol Vis Sci},
       note = {Miura, Yoko
    Huettmann, Gereon
    Orzekowsky-Schroeder, Regina
    Steven, Philipp
    Szaszak, Marta
    Koop, Norbert
    Brinkmann, Ralf
    ENG
    2013/04/06 06:00
    Invest Ophthalmol Vis Sci. 2013 Apr 4. pii: iovs.13-11808v1. doi: 10.1167/iovs.13-11808.},
       abstract = {PURPOSE: The aim of this study was to investigate the autofluorescence (AF) of the RPE with two-photon microscopy (TPM) and fluorescence lifetime imaging (FLIM) under normal and oxidative stress conditions. METHODS: Porcine RPE-choroid explants were used for investigation. The RPE-choroid tissue was preserved in a perfusion organ culture system. Oxidative stress was induced by laser photocoagulation with frequency-doubled Nd:YAG laser (532 nm) and by exposure to different concentrations (0, 1, 10 mM) of ferrous sulfate (FeSO4) for 1 hr. At indicated time points after exposure, the tissue was examined with TPM and FLIM. Intracellular reactive oxygen species around the photocoagulation lesion were detected with chloromethyl-2'7'-dichlorofluorescein diacetate (CM-H2DCFDA). Melanosomes were isolated from RPE cells and its fluorescence properties were investigated under normal and oxidized conditions. RESULTS: Under normal condition, AF in RPE cells with TPM is mostly originated from melanosomes, which has a very short fluorescence lifetime (FLT) (mean=117 ps). Under oxidative stress induced by laser irradiation and FeSO4 exposure, bright granular AF appears inside and around RPE cells, whose FLT is significantly longer (mean=1388 ps) than the FLT of the melanosome-AF. Excitation and emission peaks are found at 710-750 nm and 450-500 nm, respectively. Oxidative stress increases the fluorescence intensity of the melanosomes but does not change their FLT. CONCLUSION: TPM reveals acute oxidative stress-induced bright AF granules inside and around RPE cells which can be clearly discriminated from melanosomes by FLIM. TPM combined with FLIM is a useful tool of live-cell analysis to investigate functional alterations of the RPE.},
       year = {2013}
    }
    
  • Lauterborn, Werner and Vogel, Alfred: Shock Wave Emission by Laser Generated Bubbles. in Bubble Dynamics and Shock Waves, pp. 67-103, 2013
    BibTeX
    @inbook{Lauterborn2013,
       author = {Lauterborn, Werner and Vogel, Alfred},
       title = {Shock Wave Emission by Laser Generated Bubbles},
       booktitle = {Bubble Dynamics and Shock Waves},
       pages = {67-103},
       year = {2013}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: New High Resolution SRS with ultra broadband spectral coverage. in 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference, pp. PD_A_8 1-3, Optical Society of America, Munich, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_a,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    address = {Munich},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference},
    pages = {PD_A_8 1--3},
    publisher = {Optical Society of America},
    doi={10.1109/CLEOE-IQEC.2013.6801995},
    title = {{FDML Raman: New High Resolution SRS with ultra broadband spectral coverage}},
    url = {https://doi.org/10.1109/CLEOE-IQEC.2013.6801995},
    year = {2013}
    }
  • Choi, WooJhon and Mohler, Kathrin J and Potsaid, Benjamin and Lu, Chen D and Liu, Jonathan J and Jayaraman, Vijaysekhar and Cable, Alex E and Duker, Jay S and Huber, Robert and Fujimoto, James G: Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography. PloS one, no. 8, pp. e81499 1-8, jan,, 2013
    BibTeX Link
    @Article{HU_2013_Choi_a,
      Title                    = {{Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography}},
      Author                   = {Choi, WooJhon and Mohler, Kathrin J and Potsaid, Benjamin and Lu, Chen D and Liu, Jonathan J and Jayaraman, Vijaysekhar and Cable, Alex E and Duker, Jay S and Huber, Robert and Fujimoto, James G},
      Journal                  = {PloS one},
      Year                     = {2013},
      Month                    = jan,
      Number                   = {12},
      Pages                    = {e81499 1--8},
      Volume                   = {8},
      Doi                      = {10.1371/journal.pone.0081499}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers. in CLEO: 2013, pp. ATu3P.4 1-3, OSA, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_b,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2013},
    doi = {10.1364/CLEO_SI.2013.CTu2H.5},
    isbn = {978-1-55752-972-5},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,fiber,stimulated Raman},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_SI-2013-CTu2H.5},
    year = {2013}
    }
  • Zhang, Yaokun and Pfeiffer, Tom and Wieser, Wolfgang and Weller, Marcel and Huber, Robert and Klenzner, Thomas and Raczkowsky, Jörg and Wörn, Heinz: History compounding: a novel speckle reduction technique for OCT guided cochleostomy. in SPIE BiOS, pp. 85713H 1-3, International Society for Optics and Photonics, mar,, 2013
    BibTeX Link
    @InProceedings{HU_2013_Zhang_a,
      Title                    = {{History compounding: a novel speckle reduction technique for OCT guided cochleostomy}},
      Author                   = {Zhang, Yaokun and Pfeiffer, Tom and Wieser, Wolfgang and Weller, Marcel and Huber, Robert and Klenzner, Thomas and Raczkowsky, J\"{o}rg and W\"{o}rn, Heinz},
      Booktitle                = {SPIE BiOS},
      Year                     = {2013},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = mar,
      Pages                    = {85713H.1--3},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.2006979},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2006979}
    }
  • Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs: Intravascular optical coherence tomography imaging at 3200 frames per second. Optics letters, no. 38, pp. 1715-7, may,, 2013
    BibTeX Link
    @Article{HU_2013_Wang_a,
      Title                    = {{Intravascular optical coherence tomography imaging at 3200 frames per second}},
      Author                   = {Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs},
      Journal                  = {Optics letters},
      Year                     = {2013},
    
      Month                    = may,
      Number                   = {10},
      Pages                    = {1715--7},
      Volume                   = {38},
    
      Doi                      = {10.1364/OL.38.001715},
      ISSN                     = {1539-4794},
      Url                      = {http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-38-10-1715}
    }
  • Klein, Thomas and Andre, Raphael and Wieser, Wolfgang and Pfeiffer, Tom and Huber, Robert: Joint aperture detection for speckle reduction and increased collection efficiency in ophthalmic MHz OCT. Biomedical optics express, no. 4, pp. 619-34, apr,, 2013
    BibTeX Link
    @Article{HU_2013_Klein_b,
      Title                    = {{Joint aperture detection for speckle reduction and increased collection efficiency in ophthalmic MHz OCT}},
      Author                   = {Klein, Thomas and Andre, Raphael and Wieser, Wolfgang and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2013},
    
      Month                    = apr,
      Number                   = {4},
      Pages                    = {619--34},
      Volume                   = {4},
    
      Doi                      = {10.1364/BOE.4.000619},
      ISSN                     = {2156-7085},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-4-4-619}
    }
  • Klein, Thomas and Wieser, Wolfgang and Reznicek, Lukas and Neubauer, Aljoscha and Kampik, Anselm and Huber, Robert: Multi-MHz retinal OCT. Biomedical optics express, no. 4, pp. 1890-908, jan,, 2013
    BibTeX Link
    @Article{HU_2013_Klein_a,
      Title                    = {{Multi-MHz retinal OCT}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Reznicek, Lukas and Neubauer, Aljoscha and Kampik, Anselm and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2013},
    
      Month                    = jan,
      Number                   = {10},
      Pages                    = {1890--908},
      Volume                   = {4},
    
      Doi                      = {10.1364/BOE.4.001890},
      ISSN                     = {2156-7085},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=boe-4-10-1890}
    }
  • Eigenwillig, Christoph M and Todor, Sebastian and Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert: Picosecond pulses from a Fourier domain mode locked (FDML) laser. in 2013 Conference on Lasers &amp; Electro-Optics Europe &amp; International Quantum Electronics Conference CLEO EUROPE/IQEC, pp. 1-1, IEEE, may,, 2013
    BibTeX Link
    @InProceedings{HU_2013_Eigenwillig_a,
      Title                    = {{Picosecond pulses from a Fourier domain mode locked (FDML) laser}},
      Author                   = {Eigenwillig, Christoph M and Todor, Sebastian and Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert},
      Booktitle                = {2013 Conference on Lasers \& Electro-Optics Europe \& International Quantum Electronics Conference CLEO EUROPE/IQEC},
      Year                     = {2013},
      Month                    = may,
      Pages                    = {1--1},
      Publisher                = {IEEE},
      Doi                      = {10.1109/CLEOE-IQEC.2013.6801076},
      ISBN                     = {978-1-4799-0594-2},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=6801076}
    }
  • Eigenwillig, Christoph M and Wieser, Wolfgang and Todor, Sebastian and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert: Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers. Nature communications, no. 4, pp. 1848-1855, jan,, 2013
    BibTeX Link
    @Article{HU_2013_Eigenwillig_b,
      Title                    = {{Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers}},
      Author                   = {Eigenwillig, Christoph M and Wieser, Wolfgang and Todor, Sebastian and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert},
      Journal                  = {Nature communications},
      Year                     = {2013},
      Month                    = jan,
      Pages                    = {1848--1855},
      Volume                   = {4},
      Doi                      = {10.1038/ncomms2870},
      ISSN                     = {2041-1723},
      Url                      = {http://www.nature.com/ncomms/journal/v4/n5/abs/ncomms2870.html}
    }
  • Torzicky, Teresa and Marschall, Sebastian and Pircher, Michael and Baumann, Bernhard and Bonesi, Marco and Zotter, Stefan and Götzinger, Erich and Trasischker, Wolfgang and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Huber, Robert and Andersen, Peter and Hitzenberger, Christoph K: Retinal polarization-sensitive optical coherence tomography at 1060 nm with 350 kHz A-scan rate using an Fourier domain mode locked laser. Journal of biomedical optics, no. 18, pp. 26008-8, feb,, 2013
    BibTeX Link
    @Article{HU_2013_Torzicky_a,
      Title                    = {{Retinal polarization-sensitive optical coherence tomography at 1060 nm with 350 kHz A-scan rate using an Fourier domain mode locked laser}},
      Author                   = {Torzicky, Teresa and Marschall, Sebastian and Pircher, Michael and Baumann, Bernhard and Bonesi, Marco and Zotter, Stefan and G\"{o}tzinger, Erich and Trasischker, Wolfgang and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Huber, Robert and Andersen, Peter and Hitzenberger, Christoph K},
      Journal                  = {Journal of biomedical optics},
      Year                    = {2013},
      Month                    = feb,
      Number                   = {2},
      Pages                    = {26008--8},
      Volume                   = {18},
      Doi                      = {10.1117/1.JBO.18.2.026008},
      ISSN                     = {1560-2281},
      Url                      = {http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1568209}
    }
    
  • Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs: Ultrahigh-speed intravascular optical coherence tomography imaging at 3200 frames per second. in European Conferences on Biomedical Optics, pp. 88020O 1-2, International Society for Optics and Photonics, jun,, 2013
    BibTeX Link
    @InProceedings{HU_2013_Wang_b,
      Title                    = {{Ultrahigh-speed intravascular optical coherence tomography imaging at 3200 frames per second}},
      Author                   = {Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2013},
      Editor                   = {Bouma, Brett E and Leitgeb, Rainer A},
      Month                    = jun,
      Pages                    = {88020O 1--2 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.2032723},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2032723}
    }

2012

  • Unglert, C. I. and Warger, W. C. and Hostens, J. and Namati, E. and Birngruber, R. and Bouma, B. E. and Tearney, G. J.: Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography. Journal of Biomedical Optics, no. 17, 2012
    BibTeX Link
    @article{Unglert2012,
       author = {Unglert, C. I. and Warger, W. C. and Hostens, J. and Namati, E. and Birngruber, R. and Bouma, B. E. and Tearney, G. J.},
       title = {Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {12},
       note = {083ZB
    Times Cited:3
    Cited References Count:23},
       abstract = {Optical coherence tomography (OCT) has been increasingly used for imaging pulmonary alveoli. Only a few studies, however, have quantified individual alveolar areas, and the validity of alveolar volumes represented within OCT images has not been shown. To validate quantitative measurements of alveoli from OCT images, we compared the cross-sectional area, perimeter, volume, and surface area of matched subpleural alveoli from microcomputed tomography (micro-CT) and OCT images of fixed air-filled swine samples. The relative change in size between different alveoli was extremely well correlated (r > 0.9, P < 0.0001), but OCT images underestimated absolute sizes compared to micro-CT by 27% (area), 7% (perimeter), 46% (volume), and 25% (surface area) on average. We hypothesized that the differences resulted from refraction at the tissue-air interfaces and developed a ray-tracing model that approximates the reconstructed alveolar size within OCT images. Using this model and OCT measurements of the refractive index for lung tissue (1.41 for fresh, 1.53 for fixed), we derived equations to obtain absolute size measurements of superellipse and circular alveoli with the use of predictive correction factors. These methods and results should enable the quantification of alveolar sizes from OCT images in vivo. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.12.126015]},
       keywords = {alveolar structure
    alveolar volume
    ray-tracing
    optical coherence tomography
    refractive-index
    tissue},
       ISSN = {1083-3668},
       DOI = {Artn 126015
    Doi 10.1117/1.Jbo.17.12.126015},
       url = {<Go to ISI>://WOS:000314504400023},
       year = {2012},
       type = {Journal Article}
    }
    
  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX Link
    @article{Treumer2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {1096-0007
    Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    Journal Article
    England
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       ISSN = {0014-4835},
       DOI = {10.1016/j.exer.2012.02.011},
       year = {2012},
       type = {Journal Article}
    }
    
  • Rohde, Ingo and Brinkmann, Ralf and Theisen-Kunde, Dirk: Temporally stretched Q-switched pulses in the 2 µm spectral range. Laser Physics Letters, no. 9, pp. 808-813, 2012
    BibTeX
    @article{Rohde2012,
       author = {Rohde, Ingo and Brinkmann, Ralf and Theisen-Kunde, Dirk},
       title = {Temporally stretched Q-switched pulses in the 2 µm spectral range},
       journal = {Laser Physics Letters},
       volume = {9},
       number = {11},
       pages = {808-813},
       year = {2012}
    }
    
  • Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.: Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy. Histochem Cell Biol, no. 137, pp. 269-278, 2012
    BibTeX Link
    @article{Klinder2017,
       author = {Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.},
       title = {Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy},
       journal = {Histochem Cell Biol},
       volume = {137},
       number = {3},
       pages = {269-278},
       ISSN = {1432-119X (Electronic)
    0948-6143 (Linking)},
       DOI = {10.1007/s00418-011-0905-0},
       year = {2012},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.: Temperature-controlled retinal photocoagulation - a step toward automated laser treatment. Invest Ophthalmol Vis Sci, no. 53, pp. 3605-14, 2012
    BibTeX
    @article{Koinzer2012,
       author = {Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.},
       title = {Temperature-controlled retinal photocoagulation - a step toward automated laser treatment},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {53},
       number = {7},
       pages = {3605-14},
       note = {Using Smart Source Parsing
    Jun 14; Print 2012 Jul},
       abstract = {Purpose. Retinal laser photocoagulation carries the risk of overtreatment due to effect variation of identically applied lesions. The degree of coagulation depends on the induced temperature increase and on exposure time. We introduce temperature controlled photocoagulation (TCP), which uses optoacoustics to determine individually exposure times necessary to create reproducible lesions. Methods. Optoacoustic temperature measurement relies on pressure waves that are excited in the retinal tissue by repetitive low-energy laser pulses. Signal amplitudes correlate with tissue temperature and are detected by a transducer in the laser contact lens. We used a continuous wave (CW) photocoagulator for treatment irradiation and superimposed probe laser pulses for simultaneous temperature measurement. Optoacoustic data of 1500 lesions (rabbit) were evaluated to develop an algorithm that controls exposure times automatically in TCP. Lesion diameters of 156 TCP lesions were compared to 156 non-controlled lesions. Histology was performed after 1 hour, and 1 and 4 weeks. Results. TCP resulted in exposure times from 4 to 800 ms depending on laser power chosen. Ophthalmoscopic and histologic lesion diameters were independent of power between 14 and 200 mW. TCP lesions barely were visible with a mean diameter equal to the treatment beam (130 mum). In contrast, standard lesion diameters increased linearly and statistically significantly with power. Histology confirmed sparing of the ganglion and nerve fiber layers in TCP. Conclusions. TCP facilitates uniform retinal lesions over a wide power range. In a clinical setting, it should generate soft and reproducible lesions independently of local tissue variation and improve safety, particularly at short exposure times.},
       year = {2012}
    }
  • Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Bever, Marco and Baade, Alex and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf: Automatic temperature controlled retinal photocoagulation. Journal of Biomedical Optics, no. 17, pp. 061223, 2012
    BibTeX Link
    @article{Schlott2012,
       author = {Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Bever, Marco and Baade, Alex and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Automatic temperature controlled retinal photocoagulation},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {6},
       pages = {061223},
       keywords = {AutoPhoN},
       year = {2012}
    }
    
  • Koinzer, Stefan and Schlott, Kerstin and Portz, Lea and Ptaszynski, Lars and Baade, Alexander and Bever, Marco and Saeger, Mark and Caliebe, Amke and Denner, Renè and Birngruber, Reginald and Brinkmann, Ralf and Roider, Johann: Correlation of temperature rise and optical coherence tomography characteristics in patient retinal photocoagulation. Journal of Biophotonics, pp. n/a-n/a, 2012
    BibTeX
    @article{Koinzer,
       author = {Koinzer, Stefan and Schlott, Kerstin and Portz, Lea and Ptaszynski, Lars and Baade, Alexander and Bever, Marco and Saeger, Mark and Caliebe, Amke and Denner, Renè and Birngruber, Reginald and Brinkmann, Ralf and Roider, Johann},
       title = {Correlation of temperature rise and optical coherence tomography characteristics in patient retinal photocoagulation},
       journal = {Journal of Biophotonics},
       pages = {n/a-n/a},
       abstract = {We conducted a study to correlate the retinal temperature rise during photocoagulation to the afterward detected tissue effect in optical coherence tomography (OCT). 504 photocoagulation lesions were examined in 20 patients. The retinal temperature increase was determined in real-time during treatment based on thermoelastic tissue expansion which was probed by repetitively applied ns laser pulses. The tissue effect was examined on fundus images and OCT images of individualized lesions. We discerned seven characteristic morphological OCT lesion classes. Their validity was confirmed by increasing visibility and diameters. Mean peak temperatures at the end of irradiation ranged from approx. 60 °C to beyond 100 °C, depending on burn intensity. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)},
       keywords = {laser photocoagulation
    optoacoustics
    photocoagulation
    retinal temperature
    spectral domain optical coherence tomography
    OCT
    subthreshold
    classification},
       year = {2012}
    }
  • Brinkmann, Ralf and Koinzer, Stefan and Schlott, Kerstin and Ptaszynski, Lars and Bever, Marco and Baade, Alexander and Luft, Susanne and Miura, Yoko and Roider, Johann and Birngruber, Reginald: Real-time temperature determination during retinal photocoagulation on patients. Journal of Biomedical Optics, no. 17, pp. 061219, 2012
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, Ralf and Koinzer, Stefan and Schlott, Kerstin and Ptaszynski, Lars and Bever, Marco and Baade, Alexander and Luft, Susanne and Miura, Yoko and Roider, Johann and Birngruber, Reginald},
       title = {Real-time temperature determination during retinal photocoagulation on patients},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {6},
       pages = {061219},
       note = {Journal Article},
       year = {2012}
    }
  • Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Analysis of the signal fall-off in spectral domain optical coherence tomography systems. no. 8213, pp. 82131K, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert,
       author = {Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {Analysis of the signal fall-off in spectral domain optical coherence tomography systems},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131K},
    
    }
    
  • Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander: Feasibiliy of optical detection of soft tissue deformation during needle insertion. no. 8316, pp. 83160V, SPIE,
    BibTeX
    @inproceedings{Otte,
       author = {Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander},
       title = {Feasibiliy of optical detection of soft tissue deformation during needle insertion},
       editor = {David, R. Holmes, III and Kenneth, H. Wong},
       publisher = {SPIE},
       volume = {8316},
       pages = {83160V},
    
    }
  • Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon: Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction. Journal of Biomedical Optics, no. 17, pp. 058003, 2012
    BibTeX
    @article{Rudnitzki2012,
       author = {Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon},
       title = {Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {5},
       pages = {058003},
       year = {2012}
    }
  • Hüttmann, G. and Orzekowsky-Schröder, R.: Imaging of immune cell dynamics in small intestine and the eye by 2-photon microcopy. in 7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012),
    BibTeX
    @inproceedings{Hüttmann2012,
       author = {Hüttmann, G. and Orzekowsky-Schröder, R.},
       title = {Imaging of immune cell dynamics in small intestine and the eye  by 2-photon microcopy},
       booktitle = {7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012)},
       abstract = {Autofluorescence based 2-photon microscopy was investigated as a
    tool for studying the dynamics of immunological processes in vivo. Nearly
    all tissue components were simultaneously visible by autofluorescence and a
    nearly complete visualization of tissue architecture was possible. Within
    the tissue, immune competent cells like lymphocytes, macrophages and
    dendritic cells were visualized in their dynamic interaction with other
    cells or surrounding tissues.
    Immunological processes were studied in the small intestine and at the
    surface of the eye. Excitation and emission spectra of the different mucosal
    tissue components were quantitatively determined and a compared to the
    respective spectra of endogenous chromophores. It was shown, that by using
    only two excitation wavelengths within the tuning range of a Ti:Saphire
    laser enterocytes, antigen presenting cells and lysosomes of the small
    intestine could be discriminated based on the excitation and emission
    properties. By additionally using an intravital nuclear stain, motion of
    lymphocytes in the lamina propria and the epithelium of small intestine
    villi was quantitatively analyzed. 
    
    2-photon microscopy was also a powerful tool for studying the conjunctiva
    and cornea of the eye. Lymphocyte dynamics and uptake of microspheres or
    fluorescing heat-inactivated E. coli was followed over time in the
    conjunctiva-associated lymphoid tissue of mice. In a different mouse model
    of suture induced corneal hem- and lymphangiogenesis, immune cell migration
    into lymphatic vessels and luminal transport of individual cells was
    observed in vivo.
    
    Autofluorescence based 2-photon intravital microscopy is a valuable tool to
    study dynamic immunological processes. While tissue autofluorescence gives a
    good overview over all relevant structures and allows for discriminating
    different cell types by spectral analysis, the additional combination of
    specific staining with external dyes or fluorescent proteins is easily
    possible and enhances the potential of the technique further. },
    
    }
  • Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon: Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography. no. 8213, pp. 82131S, SPIE,
    BibTeX
    @inproceedings{Spahr2012,
       author = {Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon},
       title = {Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131S},
    
    }
  • Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim: Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy. no. 8213, pp. 821338, SPIE,
    BibTeX
    @inproceedings{Ansari2012,
       author = {Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim},
       title = {Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821338},
    
    }
  • Gehlsen, U. and Oetke, A. and Szaszak, M. and Koop, N. and Paulsen, F. and Gebert, A. and Huettmann, G. and Steven, P.: Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro. Graefes Arch Clin Exp Ophthalmol, no. 6, pp. 6, 2012
    BibTeX
    @article{Gehlsen,
       author = {Gehlsen, U. and Oetke, A. and Szaszak, M. and Koop, N. and Paulsen, F. and Gebert, A. and Huettmann, G. and Steven, P.},
       title = {Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {6},
       pages = {6},
       note = {Using Smart Source Parsing
    May},
       abstract = {BACKGROUND: Early and correct diagnosis of delayed or absent corneal epithelial wound healing is a key factor in the prevention of infection and consecutive destruction of the corneal stroma with impending irreversible visual loss. Two-photon microscopy (TPM) is a novel technology that has potential to depict epithelial cells and to evaluate cellular function by measuring autofluorescence properties such as fluorescence intensity and fluorescence lifetimes of metabolic co-factors such as NAD(P)H. METHODS: Using non-invasive TPM in a tissue-culture scratch model and an organ-culture erosion model, fluorescence intensity and fluorescence lifetimes of NAD(P)H were measured before and during closure of the epithelial wounds. Influence of temperature and selective inhibition of metabolism on intensity and lifetimes were tested additionally. RESULTS: Decrease of temperature resulted in significant increase of fluorescence lifetimes and decrease of the relative amount of free NAD(P)H due to decreased global metabolism. Increase in temperature and upregulation of glycolysis through blocking the mitochondrial electron transport chain by rotenone resulted in increased intensity, decreased lifetimes and increase in the relative amount of free NAD(P)H. Changes of lifetimes and free:protein-bound NAD(P)H ratios were similar to changes measured during wound healing in both scratch and erosion models. CONCLUSIONS: Fluorescence lifetime measurements (FLIM) detected enhancement of cellular metabolism following epithelial damage in both models. The prospective detection of cellular autofluorescence in vivo, in particular FLIM of metabolic cofactor NAD(P)H, has the potential to become an indispensible tool in clinical use to differentiate healing from non-healing epithelial cells and to evaluate effects of newly developed substances on cellular metabolism in preclinical and clinical trials.},
       year = {2012}
    }
  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX
    @article{Miura2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    eng
    England
    2012/03/06 06:00
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       year = {2012}
    }
    
  • Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon: High resolution holoscopy. no. 8213, pp. 821324, SPIE,
    BibTeX
    @inproceedings{Franke2012,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon},
       title = {High resolution holoscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821324},
    
    }
  • Iwami, Hisashi and Ptaszynski, Lars and Danicke, Veit and Brinkmann, Ralf and Miura, Yoko: Sublethal Hyperthermia-induced Vascular Endothelial Growth Factor Secretion And Its Contribution To Adoptive Response Of Retinal Pigment Epithelial Cell. Invest. Ophthalmol. Vis. Sci., no. 53, pp. 4782-, 2012
    BibTeX Link
    @article{Iwami2012,
       author = {Iwami, Hisashi and Ptaszynski, Lars and Danicke, Veit and Brinkmann, Ralf and Miura, Yoko},
       title = {Sublethal Hyperthermia-induced Vascular Endothelial Growth Factor Secretion And Its Contribution To Adoptive Response Of Retinal Pigment Epithelial Cell},
       journal = {Invest. Ophthalmol. Vis. Sci.},
       volume = {53},
       number = {6},
       pages = {4782-},
       abstract = {PurposeTo investigate temperature increase-induced secretion of vascular endothelial growth factor (VEGF) from retinal pigment epithelial (RPE) cells and its contribution to adoptive response relating to cell defence system against oxidative stress. MethodsPorcine RPE cells on 35 mm culture dish were used in the study. Thulium laser ({lambda}=1940 nm, spot size 33 mm was utilized as a heat source. Temperature increase during irradiation for different power and time setting at cell level was measured with thermocouple, and power and time setting of the experiment was determined based on this calibration. Culture medium was replaced by 1.2 ml phosphate buffer saline and then laser was irradiated with different power settings for 10 seconds, so that the peak temperature reaches from 40{degrees}C to 65{degrees}C. Cellular viability after laser irradiation was examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay immediately after irradiation. VEGF secretion was investigated with enzyme-linked immunosorbent assay (ELISA) at 2 and 24 hrs after irradiation. Contribution of a temperature-dependent calcium channel, TRPV (transient receptor potential vanilloid) channels in laser-induced VEGF secretion was investigated using TRPV channel blocker, ruthenium red (20 {micro}M). TRPV channel blocker-containing medium was replaced by the normal medium soon after laser irradiation. Hydrogen peroxide (H2O2) or advanced glycation endproduct (AGE)-was exposed after 6 hrs of laser irradiation and cell viability was examined with MTT assay. ResultsPeak temperature threshold for immediate RPE cell death was found around 55 {degrees}C with our irradiation setting. VEGF secretion was increased after sub-lethal irradiation in power-dependent manner, which was partially suppressed by TRPV channel blocker. Sublethal laser irradiation reduced H2O2 and AGE-induced cell death and this effect was smaller in the cells treated with TRPV channel inhibitor during laser irradiation. ConclusionsSublethal temperature increase-induced VEGF production might contribute to the enhancement of RPE cell defence system against oxidative stress.},
       url = {http://abstracts.iovs.org/cgi/content/abstract/53/6/4782},
       year = {2012},
       type = {Journal Article}
    }
    
  • Hillmann, D. and Bonin, T. and Luhrs, C. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Common approach for compensation of axial motion artifacts in swept-source OCT and dispersion in Fourier-domain OCT. Opt Express, no. 20, pp. 6761-6776, Mar, 2012
    BibTeX
    @article{Hillmann2012,
       author={Hillmann, D.  and Bonin, T.  and Luhrs, C.  and Franke, G.  and Hagen-Eggert, M.  and Koch, P.  and Huttmann, G. },
       title={{{C}ommon approach for compensation of axial motion artifacts in swept-source {O}{C}{T} and dispersion in {F}ourier-domain {O}{C}{T}}},
       journal={Opt Express},
       year={2012},
       volume={20},
       number={6},
       pages={6761--6776},
       month={Mar}
    }
    
  • Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.: Confocal endomicroscopy of the larynx. no. 8207, pp. 82072H, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.},
       title = {Confocal endomicroscopy of the larynx},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian Jet-Fei, Wong and Justus, F. Ilgner and Krzysztof, Izdebski and Melissa, J. Suter and Stephen, Lam and Matthew, Brenner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, Madsen and Anita, Mahadevan-Jansen and Jansen, E. Duco and Andreas, Mandelis},
       publisher = {SPIE},
       volume = {8207},
       pages = {82072H},
    
    }
  • Mueller, H. H. and Ptaszynski, L. and Schlott, K. and Debbeler, C. and Bever, M. and Koinzer, S. and Birngruber, R. and Brinkmann, R. and Huettmann, G.: Imaging thermal expansion and retinal tissue changes during photocoagulation by high speed OCT. Biomedical Optics Express, no. 3, pp. 1025-1046, 2012
    BibTeX Link
    @article{Müller2012,
       author = {Mueller, H. H. and Ptaszynski, L. and Schlott, K. and Debbeler, C. and Bever, M. and Koinzer, S. and Birngruber, R. and Brinkmann, R. and Huettmann, G.},
       title = {Imaging thermal expansion and retinal tissue changes during photocoagulation by high speed OCT},
       journal = {Biomedical Optics Express},
       volume = {3},
       number = {5},
       pages = {1025-1046},
       note = {935RH
    Times Cited:8
    Cited References Count:37},
       abstract = {Visualizing retinal photocoagulation by real-time OCT measurements may considerably improve the understanding of thermally induced tissue changes and might enable a better reproducibility of the ocular laser treatment. High speed Doppler OCT with 860 frames per second imaged tissue changes in the fundus of enucleated porcine eyes during laser irradiation. Tissue motion, measured by Doppler OCT with nanometer resolution, was correlated with the temperature increase, which was measured non-invasively by optoacoustics. In enucleated eyes, the increase of the OCT signal near the retinal pigment epithelium (RPE) corresponded well to the macroscopically visible whitening of the tissue. At low irradiance, Doppler OCT revealed additionally a reversible thermal expansion of the retina. At higher irradiance additional movement due to irreversible tissue changes was observed. Measurements of the tissue expansion were also possible in vivo in a rabbit with submicrometer resolution when global tissue motion was compensated. Doppler OCT may be used for spatially resolved measurements of retinal temperature increases and thermally induced tissue changes. It can play an important role in understanding the mechanisms of photocoagulation and, eventually, lead to new strategies for retinal laser treatments. (c) 2012 Optical Society of America},
       keywords = {optical coherence tomography
    laser photocoagulation
    vein occlusion
    management
    diseases
    fundus
    blood},
       ISSN = {2156-7085},
       url = {<Go to ISI>://WOS:000303537400018},
       year = {2012},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon: Efficient holoscopy image reconstruction. Opt. Express, no. 20, pp. 21247-21263, 2012
    BibTeX
    @article{Hillmann2012,
       author = {Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon},
       title = {Efficient holoscopy image reconstruction},
       journal = {Opt. Express},
       volume = {20},
       number = {19},
       pages = {21247-21263},
       abstract = {Holoscopy is a tomographic imaging technique that combines digital holography and Fourier-domain optical coherence tomography (OCT) to gain tomograms with diffraction limited resolution and uniform sensitivity over several Rayleigh lengths. The lateral image information is calculated from the spatial interference pattern formed by light scattered from the sample and a reference beam. The depth information is obtained from the spectral dependence of the recorded digital holograms. Numerous digital holograms are acquired at different wavelengths and then reconstructed for a common plane in the sample. Afterwards standard Fourier-domain OCT signal processing achieves depth discrimination. Here we describe and demonstrate an optimized data reconstruction algorithm for holoscopy which is related to the inverse scattering reconstruction of wavelength-scanned full-field optical coherence tomography data. Instead of calculating a regularized pseudoinverse of the forward operator, the recorded optical fields are propagated back into the sample volume. In one processing step the high frequency components of the scattering potential are reconstructed on a non-equidistant grid in three-dimensional spatial frequency space. A Fourier transform yields an OCT equivalent image of the object structure. In contrast to the original holoscopy reconstruction with backpropagation and Fourier transform with respect to the wavenumber, the required processing time does neither depend on the confocal parameter nor on the depth of the volume. For an imaging NA of 0.14, the processing time was decreased by a factor of 15, at higher NA the gain in reconstruction speed may reach two orders of magnitude.},
       keywords = {Image processing
    Optical coherence tomography
    Digital holography},
       year = {2012}
    }
    
  • Todor, Sebastian and Biedermann, Benjamin and Huber, Robert and Jirauschek, Christian: Balance of physical effects causing stationary operation of Fourier domain mode-locked lasers. Journal of the Optical Society of America B, no. 29, pp. 656-664, OSA, 2012
    BibTeX Link
    @Article{HU_2012_Todor_a,
      Title                    = {{Balance of physical effects causing stationary operation of Fourier domain mode-locked lasers}},
      Author                   = {Todor, Sebastian and Biedermann, Benjamin and Huber, Robert and Jirauschek, Christian},
      Journal                  = {Journal of the Optical Society of America B},
      Year                     = {2012},
      Number                   = {4},
      Pages                    = {656--664 },
      Volume                   = {29},
    
      Doi                      = {10.1364/JOSAB.29.000656},
      Publisher                = {OSA},
      Url                      = {http://josab.osa.org/abstract.cfm?URI=josab-29-4-656}
    }
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Torzicky, Teresa and Pircher, Michael and Biedermann, Benjamin R and Pedersen, Christian and Hitzenberger, Christoph K and Huber, Robert and Andersen, Peter E: Broadband Fourier domain mode-locked laser for optical coherence tomography at 1060 nm. in SPIE BiOS, pp. 82130R 1-3, International Society for Optics and Photonics, feb,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Marschall_b,
      Title                    = {{Broadband Fourier domain mode-locked laser for optical coherence tomography at 1060 nm}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Torzicky, Teresa and Pircher, Michael and Biedermann, Benjamin R and Pedersen, Christian and Hitzenberger, Christoph K and Huber, Robert and Andersen, Peter E},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82130R 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.906148},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.906148}
    }
  • Wieser, Wolfgang and Palte, Gesa and Eigenwillig, Christoph M and Biedermann, Benjamin R and Pfeiffer, Tom and Huber, Robert: Chromatic polarization effects of swept waveforms in FDML lasers and fiber spools. Optics express, no. 20, pp. 9819-32, apr,, 2012
    BibTeX Link
    @Article{HU_2012_Wieser_a,
      Title                    = {{Chromatic polarization effects of swept waveforms in FDML lasers and fiber spools}},
      Author                   = {Wieser, Wolfgang and Palte, Gesa and Eigenwillig, Christoph M and Biedermann, Benjamin R and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2012},
    
      Month                    = apr,
      Number                   = {9},
      Pages                    = {9819--32},
      Volume                   = {20},
    
      Doi                      = {10.1364/OE.20.009819},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-9-9819\&id=231991}
    }
  • Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A: Coherence length extension of Fourier domain mode locked lasers. in SPIE BiOS, pp. 82130O 1-3, International Society for Optics and Photonics, feb,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Adler_a,
      Title                    = {{Coherence length extension of Fourier domain mode locked lasers}},
      Author                   = {Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82130O 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.908148},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.908148}
    }
  • Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer: Deep skin structural and microcirculation imaging with extended-focus OCT. in SPIE BiOS, pp. 82070B-82070B-7, International Society for Optics and Photonics, feb,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Blatter_e,
      Title                    = {{Deep skin structural and microcirculation imaging with extended-focus OCT}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Month                    = feb,
      Pages                    = {82070B--82070B--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.909830},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1310560}
    }
  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert: Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment. in Conference on Lasers and Electro-Optics 2012, pp. JTh3J.2 1-3, OSA, Washington, D.C., 2012
    BibTeX Link
    @InProceedings{HU_2012_Wieser_c,
      Title                    = {{Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert},
      Booktitle                = {Conference on Lasers and Electro-Optics 2012},
      Year                     = {2012},
    
      Address                  = {Washington, D.C.},
      Pages                    = {JTh3J.2 1--3},
      Publisher                = {OSA},
    
      Doi                      = {10.1364/CLEO_AT.2012.JTh3J.2},
      ISBN                     = {978-1-55752-943-5},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_AT-2012-JTh3J.2}
    }
  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert: Extended coherence length megahertz FDML and its application for anterior segment imaging. Biomedical optics express, no. 3, pp. 2647-57, oct,, 2012
    BibTeX Link
    @Article{HU_2012_Wieser_b,
      Title                    = {{Extended coherence length megahertz FDML and its application for anterior segment imaging}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = oct,
      Number                   = {10},
      Pages                    = {2647--57},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002647},
      ISSN                     = {2156-7085},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-10-2647}
    }
    
  • Bonesi, Marco and Sattmann, Harald and Torzicky, Teresa and Zotter, Stefan and Baumann, Bernhard and Pircher, Michael and Götzinger, Erich and Eigenwillig, Christoph and Wieser, Wolfgang and Huber, Robert and Hitzenberger, Christoph K: High-speed polarization sensitive optical coherence tomography scan engine based on Fourier domain mode locked laser. Biomedical optics express, no. 3, pp. 2987-3000, nov,, 2012
    BibTeX Link
    @Article{HU_2012_Bonesi_a,
      Title                    = {{High-speed polarization sensitive optical coherence tomography scan engine based on Fourier domain mode locked laser}},
      Author                   = {Bonesi, Marco and Sattmann, Harald and Torzicky, Teresa and Zotter, Stefan and Baumann, Bernhard and Pircher, Michael and G\"{o}tzinger, Erich and Eigenwillig, Christoph and Wieser, Wolfgang and Huber, Robert and Hitzenberger, Christoph K},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = nov,
      Number                   = {11},
      Pages                    = {2987--3000},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002987},
      ISSN                     = {2156-7085},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-11-2987}
    }
  • Marschall, Sebastian and Torzicky, Teresa and Klein, Thomas and Wieser, Wolfgang and Pircher, Michael and Götzinger, Erich and Zotter, Stefan and Bonesi, Marco and Biedermann, Benjamin and Pedersen, Christian and Huber, Robert and Hitzenberger, Christoph and Andersen, Peter: High-speed polarization-sensitive OCT at 1060 nm using a Fourier domain mode-locked swept source. in SPIE Photonics Europe, pp. 84271D-84271D-6, International Society for Optics and Photonics, jun,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Marschall_a,
      Title                    = {{High-speed polarization-sensitive OCT at 1060 nm using a Fourier domain mode-locked swept source}},
      Author                   = {Marschall, Sebastian and Torzicky, Teresa and Klein, Thomas and Wieser, Wolfgang and Pircher, Michael and G\"{o}tzinger, Erich and Zotter, Stefan and Bonesi, Marco and Biedermann, Benjamin and Pedersen, Christian and Huber, Robert and Hitzenberger, Christoph and Andersen, Peter},
      Booktitle                = {SPIE Photonics Europe},
      Year                     = {2012},
      Editor                   = {Popp, J\"{u}rgen and Drexler, Wolfgang and Tuchin, Valery V and Matthews, Dennis L},
      Month                    = jun,
      Pages                    = {84271D--84271D--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.922313},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1219832}
    }
  • Blatter, Cedric and Weingast, Jessika and Alex, Aneesh and Grajciar, Branislav and Wieser, Wolfgang and Drexler, Wolfgang and Huber, Robert and Leitgeb, Rainer A: In situ structural and microangiographic assessment of human skin lesions with high-speed OCT. Biomedical optics express, no. 3, pp. 2636-46, OSA, oct,, 2012
    BibTeX Link
    @Article{HU_2012_Blatter_a,
      Title                    = {{In situ structural and microangiographic assessment of human skin lesions with high-speed OCT}},
      Author                   = {Blatter, Cedric and Weingast, Jessika and Alex, Aneesh and Grajciar, Branislav and Wieser, Wolfgang and Drexler, Wolfgang and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = oct,
      Number                   = {10},
      Pages                    = {2636--46},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002636},
      ISSN                     = {2156-7085},
      Pmid                     = {23082302},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-10-2636 http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3469999\&tool=pmcentrez\&rendertype=abstract}
    }
  • Blatter, Cedric and Grajciar, Branislav and Zou, Pu and Wieser, Wolfgang and Verhoef, Aart-Jan and Huber, Robert and Leitgeb, Rainer A: Intrasweep phase-sensitive optical coherence tomography for noncontact optical photoacoustic imaging. Optics letters, no. 37, pp. 4368-70, OSA, nov,, 2012
    BibTeX Link
    @Article{HU_2012_Blatter_b,
      Title                    = {{Intrasweep phase-sensitive optical coherence tomography for noncontact optical photoacoustic imaging}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Zou, Pu and Wieser, Wolfgang and Verhoef, Aart-Jan and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Optics letters},
      Year                     = {2012},
    
      Month                    = nov,
      Number                   = {21},
      Pages                    = {4368--70},
      Volume                   = {37},
    
      Doi                      = {10.1364/OL.37.004368},
      ISSN                     = {1539-4794},
      Pmid                     = {23114298},
      Publisher                = {OSA},
      Url                      = {http://ol.osa.org/abstract.cfm?URI=ol-37-21-4368 http://www.ncbi.nlm.nih.gov/pubmed/23114298}
    }
  • Klein, Thomas and Wieser, Wolfgang and Andre, Raphael and Pfeiffer, Tom and Eigenwillig, Christoph M and Huber, Robert: Multi-MHz FDML OCT: snapshot retinal imaging at 6.7 million axial-scans per second. in SPIE BiOS, pp. 82131E 1-3, International Society for Optics and Photonics, feb,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Klein_b,
      Title                    = {{Multi-MHz FDML OCT: snapshot retinal imaging at 6.7 million axial-scans per second}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Andre, Raphael and Pfeiffer, Tom and Eigenwillig, Christoph M and Huber, Robert},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82131E 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.908798},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.908798}
    }
  • Klein, Thomas and Wieser, Wolfgang and Huber, Robert: OPTICAL COHERENCE TOMOGRAPHY/HIGH-SPEED BIOMEDICAL IMAGING: No speed limit: The multi-megahertz approach to optical coherence tomography. BioOptics World, no. 5, pp. 28-32, 2012
    BibTeX
    @Article{HU_2012_Klein_a,
      Title                    = {{OPTICAL COHERENCE TOMOGRAPHY/HIGH-SPEED BIOMEDICAL IMAGING: No speed limit: The multi-megahertz approach to optical coherence tomography}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {BioOptics World},
      Year                     = {2012},
      Number                   = {1},
      Pages                    = {28--32},
      Volume                   = {5},
      Doi                      = {http://www.bioopticsworld.com/articles/print/volume-5/issue-1/features/the-multi-megahertz-approach-to-optical-coherence-tomography.html},
      Url                      = {http://www.bioopticsworld.com/articles/print/volume-5/issue-1/features/the-multi-megahertz-approach-to-optical-coherence-tomography.html}
    }
  • Blatter, Cedric and Grajciar, Branislav and Hermann, Boris and Huber, Robert and Drexler, Wolfgang and Leitgeb, Rainer A: Simultaneous dark-bright field swept source OCT for ultrasound detection. in SPIE BiOS, pp. 82131M 1-3, International Society for Optics and Photonics, feb,, 2012
    BibTeX Link
    @InProceedings{HU_2012_Blatter_d,
      Title                    = {{Simultaneous dark-bright field swept source OCT for ultrasound detection}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Hermann, Boris and Huber, Robert and Drexler, Wolfgang and Leitgeb, Rainer A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82131M 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.911443},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.911443}
    }
  • Blatter, Cedric and Klein, Thomas and Grajciar, Branislav and Schmoll, Tilman and Wieser, Wolfgang and Andre, Raphael and Huber, Robert and Leitgeb, Rainer A: Ultrahigh-speed non-invasive widefield angiography. Journal of biomedical optics, no. 17, pp. 070505 1-3, jul,, 2012
    BibTeX Link
    @Article{HU_2012_Blatter_c,
      Title                    = {{Ultrahigh-speed non-invasive widefield angiography}},
      Author                   = {Blatter, Cedric and Klein, Thomas and Grajciar, Branislav and Schmoll, Tilman and Wieser, Wolfgang and Andre, Raphael and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Journal of biomedical optics},
      Year                     = {2012},
    
      Month                    = jul,
      Number                   = {7},
      Pages                    = {070505 1--3 },
      Volume                   = {17},
    
      Doi                      = {10.1117/1.JBO.17.7.070505},
      ISSN                     = {1560-2281},
      Url                      = {http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1351611}
    }

2011

  • Brinkmann, R and Koinzer, S and Schlott, K and Ptaszynski, L and Bever, M and Baade, A and Miura, Y and Birngruber, R and Roider, J: Realtime temperature determination during retinal photocoagulation on patients.. Proc SPIE, no. 7885, pp. 78850R, 2011
    BibTeX
    @article{Brinkmann2011,
       author = {Brinkmann, R and Koinzer, S and Schlott, K and Ptaszynski, L and Bever, M and Baade, A and Miura, Y and Birngruber, R and Roider, J},
       title = {Realtime temperature determination during retinal photocoagulation on patients.},
       journal = {Proc SPIE},
       volume = {7885},
       pages = {78850R},
       abstract = {Retinal photocoagulation is a long time established treatment for a variety of retinal diseases, most commonly applied for diabetic macular edema and diabetic retinopathy. The damage extent of the induced thermal coagulations depend on the temperature increase and the time of irradiation. So far, the induced temperature rise is unknown due to intraocular variations in light transmission and scattering and RPE/choroidal pigmentation, which can vary inter- and intraindividually by more than a factor of four. Thus in clinical practice, often stronger and deeper coagulations are applied than therapeutically needed, which lead to extended retinal damage and strong pain perception. The final goal of this project focuses on a dosimetry control, which automatically generates a desired temperature profile and thus coagulation strength for every individual coagulation spot, ideally unburden the ophthalmologist from any laser settings. In this paper we present the first realtime temperature measurements achieved on patients during retinal photocoagulation by means of an optoacoustic method, making use of the temperature dependence of the thermal expansion coefficient of retinal tissue. Therefore, nanosecond probe laser pulses are repetitively and simultaneously applied with the treatment radiation in order to excite acoustic waves, which are detected at the cornea with an ultrasonic transducer embedded in the contact lens and then are processed by PC.},
       url = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=732381},
       year = {2011},
       type = {Journal Article}
    }
    
  • Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.: The proliferation marker Ki-67 as novel molecular target for PDT. Photodiagnosis and Photodynamic Therapy, no. 8, pp. 129, 2011
    BibTeX Link
    @article{Rahmanzadeh2011,
       author = {Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.},
       title = {The proliferation marker Ki-67 as novel molecular target for PDT},
       journal = {Photodiagnosis and Photodynamic Therapy},
       volume = {8},
       number = {2},
     pages = {129},
       DOI = {10.1016/j.pdpdt.2011.03.025},
       year = {2011},
       type = {Journal Article}
    }
    
  • Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.: Holoscopy-holographic optical coherence tomography. Opt Lett, no. 36, pp. 2390-2, 2011
    BibTeX Link Link
    @article{Hillmann2011,
       author = {Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.},
       title = {Holoscopy--holographic optical coherence tomography},
       journal = {Opt Lett},
       volume = {36},
       number = {13},
       pages = {2390-2},
       abstract = {Scanning optical coherence tomography (OCT) is limited in sensitivity and resolution by the restricted focal depth of the confocal detection scheme. Holoscopy, a combination of holography and Fourier-domain full-field OCT, is proposed as a way to detect photons from all depths of a sample volume simultaneously with uniform sensitivity and lateral resolution, even at high NAs. By using the scalar diffraction theory, as frequently applied in digital holographic imaging, we fully reconstruct the object field with depth-invariant imaging quality. In vivo imaging of human skin is demonstrated with an image quality comparable to conventionally scanned OCT.},
       keywords = {Fingers
    Fourier Analysis
    Holography/*methods
    Humans
    Photons
    Tomography, Optical Coherence/*methods},
       ISSN = {1539-4794 (Electronic)
    0146-9592 (Linking)},
       DOI = {10.1364/OL.36.002390},
       url = {http://www.ncbi.nlm.nih.gov/pubmed/21725421},
       year = {2011},
       type = {Journal Article}
    }
    
  • Horstmann, Jens and Baade, Alexander and Brinkmann, Ralf: Photoacoustic blood vessel detection during surgical laser interventions. no. 8092, pp. 80920Z-80920Z-6, SPIE ECBO,
    BibTeX Link
    @inproceedings{Horstmann2011,
       author = {Horstmann, Jens and Baade, Alexander and Brinkmann, Ralf},
       title = {Photoacoustic blood vessel detection during surgical laser interventions},
       publisher = {SPIE ECBO},
       volume = {8092},
       pages = {80920Z-80920Z-6},
       note = {10.1117/12.889635},
       abstract = {This paper presents a discussion about the potential of photoacoustics with regard to its application in surgical assistance during minimally invasive, laser assisted interventions. Aim of the work is the detection of obscured large blood vessels in order to prevent unintentional dissection. Based on spectroscopic investigations of the target tissue (liver), a wavelength for the photoacoustic excitation laser was chosen with respect to a high absorption contrast between the vessel and the surrounding liver tissue. An experimental setup featuring a simple liver model is created. Preliminary results show, that vessels with a diameter of 2 mm can be detected up to a distance of 1 mm from the treatment fibre. It is shown, that detection of acoustic waves induced inside liver is feasible over distances higher than 10 cm.},
       url = {http://dx.doi.org/10.1117/12.889635},
       type = {Conference Proceedings}
    }
    
  • Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba: Targeted molecular effects through laser-irradiated nanoabsorbers. in Proc. SPIE,
    BibTeX Link
    @inproceedings{Hüttmann2011,
       author = {Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba},
     booktitle = {Proc. SPIE},
       title = {Targeted molecular effects through laser-irradiated nanoabsorbers},
       editor = {Newsroom, SPIE},
       DOI = {DOI: 10.1117/2.1201102.003548},
       type = {Conference Proceedings}
    }
    
  • Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.: OCT in the field of laryngology: further perspectives. no. 7883, pp. 78831W, SPIE,
    BibTeX
    @inproceedings{Just2011,
       author = {Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.},
       title = {OCT in the field of laryngology: further perspectives},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7883},
       pages = {78831W},
    
    }
  • Alex, Aneesh and Weingast, Jessika and Hofer, Bernd and Eibl, Matthias and Binder, Michael and Pehamberger, Hubert and Drexler, Wolfgang and Povazay, Boris: 3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers. Imaging in Medicine, no. 3, pp. 653-674, Future Medicine, 2011
    BibTeX
    @article{alex20113d,
      title={3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers},
      author={Alex, Aneesh and Weingast, Jessika and Hofer, Bernd and Eibl, Matthias and Binder, Michael and Pehamberger, Hubert and Drexler, Wolfgang and Povazay, Boris},
      journal={Imaging in Medicine},
      volume={3},
      number={6},
      pages={653--674},
      year={2011},
      publisher={Future Medicine},
      Doi={10.2217/iim.11.62}
    }
    
  • Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.: Diffusion-sensitive Fourier-domain optical coherence tomography. no. 7889, pp. 78892B, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert2011,
       author = {Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.},
       title = {Diffusion-sensitive Fourier-domain optical coherence tomography},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78892B},
    
    }
  • Hillmann, Dierck and Luhrs, Christian and Bonin, Tim and Koch, Peter and Vogel, Alfred and Huttmann, Gereon: Holoscopy: holographic optical coherence tomography. no. 8091, pp. 80911H, Proc. SPIE,
    BibTeX Link
    @inproceedings{Hillmann2011,
       author = {Hillmann, Dierck and Luhrs, Christian  and Bonin, Tim  and Koch, Peter and Vogel, Alfred  and Huttmann, Gereon },
       title = {Holoscopy: holographic optical coherence tomography},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {Proc. SPIE},
       volume = {8091},
       pages = {80911H},
       url = {http://link.aip.org/link/?PSI/8091/80911H/1
    http://dx.doi.org/10.1117/12.889485},
       type = {Conference Proceedings}
    }
    
  • Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon: Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina. no. 7889, pp. 788906, SPIE,
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon},
       title = {Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {788906},
    
    }
  • Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.: Diffusion-sensitive Fourier-domain optical coherence tomography. no. 7889, pp. 78892B, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert2011,
       author = {Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.},
       title = {Diffusion-sensitive Fourier-domain optical coherence tomography},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78892B},
    
    }
  • Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon: Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT. no. 7889, pp. 78890E, SPIE,
    BibTeX
    @inproceedings{Müller2011,
       author = {Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon},
       title = {Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78890E},
    
    }
  • Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi: A Case of Retinal Injury By A Violet Light-Emitting Diode. Retinal Cases and Brief Reports, no. 5, pp. 223-226 10.1097/ICB.0b013e3181e180d5, 2011
    BibTeX
    @article{Obana,
       author = {Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi},
       title = {A Case of Retinal Injury By A Violet Light-Emitting Diode},
       journal = {Retinal Cases and Brief Reports},
       volume = {5},
       number = {3},
       pages = {223-226 10.1097/ICB.0b013e3181e180d5},
       abstract = {Purpose: To describe the first case of retinal injury by a misuse of a toy using light-emitting diode. Methods: A 15-year-old male Japanese student received irradiation on his right eye by a 5 mW light-emitting diode of 410 nm wavelength for 20 seconds in 2 days. He noticed decreased vision and central scotoma approximately 2 weeks later from these events. The mechanism of injury was evaluated from the estimated irradiance on the retina by comparison with experimental threshold data published. Results: Chorioretinal atrophy with visual loss and central scotoma has remained on the fovea. The patient received an estimated dose of 1.58 J/cm2 2 times, which was close to the experimentally determined radiant exposure for photochemical injury of rat retina. Conclusion: The violet light from light-emitting diodes is a potential hazard for the retina, and thus, direct viewing into the beam should be avoided. Children, especially, should not be allowed to play with such toys without being carefully instructed about their proper use and fully supervised.},
       keywords = {black light
    light-emitting diode
    photochemical damage
    retinal injury
    visual disturbance.
    01271216-201100530-00011},
       year = {2011}
    }
    
  • Klatt, C. and Saeger, M. and Oppermann, T. and Porksen, E. and Treumer, F. and Hillenkamp, J. and Fritzer, E. and Brinkmann, R. and Birngruber, R. and Roider, J.: Selective retina therapy for acute central serous chorioretinopathy. Br J Ophthalmol, no. 95, pp. 83-8, 2011
    BibTeX
    @article{Klatt,
       author = {Klatt, C. and Saeger, M. and Oppermann, T. and Porksen, E. and Treumer, F. and Hillenkamp, J. and Fritzer, E. and Brinkmann, R. and Birngruber, R. and Roider, J.},
       title = {Selective retina therapy for acute central serous chorioretinopathy},
       journal = {Br J Ophthalmol},
       volume = {95},
       number = {1},
       pages = {83-8},
       note = {Using Smart Source Parsing
    Jan; Epub 2010 Jun 15},
       abstract = {AIMS: To evaluate selective retina therapy (SRT) as a treatment of acute central serous chorioretinopathy. METHODS: 30 eyes of 30 patients with central serous chorioretinopathy of at least a 3 months' duration were recruited. 14 eyes were randomised to an SRT group (Q-switched neodymium-doped yttrium lithium fluoride (Nd:YLF) laser, wavelength 527 nm, t=1.7 mus, energy 100-370 muJ, spot diameter 200 mum, pulse repetition rate 100 Hz,) and 16 eyes to a control group. After 3 months of follow-up, patients in the control group with persistence of subretinal fluid (SRF) were allocated to a cross-over group, treated with SRT and followed up for further 3 months. The main outcome measures were change of best-corrected Early Treatment Diabetic Retinopathy Study visual acuity (BCVA) and SRF. RESULTS: At 3 months of follow-up, the mean (SD) improvement of BCVA was significantly greater after SRT than in the control group: 12.7 (7.2) versus 6.3 (8.9) letters (p=0.04). SRF had decreased significantly more after SRT as compared with that the control group: 203 (136) mum versus 41 (150) mum (p=0.005). In eight eyes allocated to the cross-over group, the mean BCVA had increased during 3 months of follow up before SRT by 1.4 (5.2) letters and continued to increase during 3 months following SRT by 7.4 (6.3) letters, while SRF increased by 39.5 (160.2) mum before SRT and decreased by 151.5 (204.9) mum after SRT. In six of the eight eyes, SRF had completely resolved 3 months after SRT. CONCLUSIONS: SRT appears to expedite functional recovery and the re-absorption of SRF as compared with that in untreated controls. A larger prospective, randomised phase 3 confirmative patient study is warranted. TRIAL REGISTRATION NUMBER: NCT00987077.},
       year = {2011}
    }
  • Bonin, Tim and Koch, Peter and Huttmann, Gereon: Comparison of fast swept source full-field OCT with conventional scanning OCT. no. 8091, pp. 80911K, SPIE,
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Koch, Peter and Huttmann, Gereon},
       title = {Comparison of fast swept source full-field OCT with conventional scanning OCT},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {SPIE},
       volume = {8091},
       pages = {80911K},
    
    }
  • Miura, Yoko: Retinal pigment epithelium-choroid organ culture. Expert Rev Ophthalmol, no. 6, pp. 669-680, 2011
    BibTeX
    @article{Miura2011,
       author = {Miura, Yoko},
       title = {Retinal pigment epithelium-choroid organ culture},
       journal = {Expert Rev  Ophthalmol},
       volume = {6},
       number = {6},
       pages = {669-680},
       abstract = {The retinal pigment epithelium (RPE) plays a vital role in retinal function, and therefore studies on RPE provide a significant benefit for our visual function. In order to obtain useful information from study results, choosing the suitable experimental model for each purpose of study is of great importance. Although RPE cell cultures are widely used, cells in cell culture have significantly different phenotypical characteristics from in vivo cells. The advantage of using native tissue is that cells in the tissue have close biological properties to in vivo conditions. This review describes basic characteristics of native RPE–choroid tissues in comparison to RPE cells in cell culture and introduces the possibility of preserving tissues in different culture systems. Advantages and disadvantages of organ culture and suitable studies with recent study results are also introduced.},
       year = {2011}
    }
  • Vogel, Alfred and Venugopalan, Vasan: Pulsed Laser Ablation of Soft Biological Tissues. in Optical-Thermal Response of Laser-Irradiated Tissue, pp. 551-615, 2011
    BibTeX
    @inbook{Vogel,
       author = {Vogel, Alfred and Venugopalan, Vasan},
       title = {Pulsed Laser Ablation of Soft Biological Tissues},
       booktitle = {Optical-Thermal Response of Laser-Irradiated Tissue},
       pages = {551-615},
       year = {2011}
    }
    
  • Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan: Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell. PLoS Pathog, no. 7, pp. e1002108, 2011
    BibTeX
    @article{Szaszak,
       author = {Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan},
       title = {Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell},
       journal = {PLoS Pathog},
       volume = {7},
       number = {7},
       pages = {e1002108},
       abstract = {<title>Author Summary</title> <p>Separate analysis of host and pathogen metabolic changes in intracellular <italic>C. trachomatis</italic> infections is arduous and has not been comprehensively realized so far. A more detailed understanding about the metabolic activity and needs of <italic>C. trachomatis</italic> and its specific interactions with the host cell would be the basis for the development of novel treatment strategies. We therefore applied fluorescence lifetime imaging (FLIM) of the metabolic coenzymes NAD(P)H using two-photon microscopy to directly visualize metabolic changes of host cells and pathogens in living cells. NAD(P)H fluorescence was detected both on the chlamydial inclusion membrane and inside the inclusion. Interestingly, changes in chlamydial growth and progeny induced by glucose starvation and IFN? treatment were directly linked to significant changes of the NAD(P)H fluorescence lifetimes inside the inclusions. Furthermore, measurement of the NAD(P)H fluorescence lifetime in the host cell nucleus revealed that infected cells were programmed for starvation during the metabolically active phase of intracellular chlamydial growth. Our findings highlight for the first time a direct interaction between host and pathogen metabolism in intracellular bacterial infections that exceeds sole competition for nutrients. In conclusion, fluorescence lifetime imaging of NAD(P)H by two-photon microscopy enables real-time analysis of metabolic host-pathogen interactions in intracellular infections with high spatial and temporal resolution.</p>},
       year = {2011}
    }
  • Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus: Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels. PLoS One, no. 6, pp. e26253, 2011
    BibTeX
    @article{Steven2011,
       author = {Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus},
       title = {Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels},
       journal = {PLoS One},
       volume = {6},
       number = {10},
       pages = {e26253},
       abstract = {<sec> <title>Background</title> <p>The role of lymphatic vessels in tissue and organ transplantation as well as in tumor growth and metastasis has drawn great attention in recent years.</p> </sec> <sec> <title>Methodology/Principal Findings</title> <p>We now developed a novel method using non-invasive two-photon microscopy to simultaneously visualize and track specifically stained lymphatic vessels and autofluorescent adjacent tissues such as collagen fibrils, blood vessels and immune cells in the mouse model of corneal neovascularization in vivo. The mouse cornea serves as an ideal tissue for this technique due to its easy accessibility and its inducible and modifiable state of pathological hem- and lymphvascularization.</p> <p>Neovascularization was induced by suture placement in corneas of Balb/C mice. Two weeks after treatment, lymphatic vessels were stained intravital by intrastromal injection of a fluorescently labeled LYVE-1 antibody and the corneas were evaluated in vivo by two-photon microscopy (TPM). Intravital TPM was performed at 710 nm and 826 nm excitation wavelengths to detect immunofluorescence and tissue autofluorescence using a custom made animal holder. Corneas were then harvested, fixed and analyzed by histology.</p> <p>Time lapse imaging demonstrated the first in vivo evidence of immune cell migration into lymphatic vessels and luminal transport of individual cells. Cells immigrated within 1–5.5 min into the vessel lumen. Mean velocities of intrastromal corneal immune cells were around 9 µm/min and therefore comparable to those of T-cells and macrophages in other mucosal surfaces.</p> </sec> <sec> <title>Conclusions</title> <p>To our knowledge we here demonstrate for the first time the intravital real-time transmigration of immune cells into lymphatic vessels. Overall this study demonstrates the valuable use of intravital autofluorescence two-photon microscopy in the model of suture-induced corneal vascularizations to study interactions of immune and subsequently tumor cells with lymphatic vessels under close as possible physiological conditions.</p> </sec>},
       year = {2011}
    }
  • Theisen-Kunde, D. and Tedsen, S. and Doehn, C. and Jocham, D. and Kausch von Schmeling, I.: Comparison between a 1.92-mum fiber laser and a standard HF-dissection device for nephron-sparing kidney resection in a porcine in vivo study. Lasers Med Sci, no. 26, pp. 509-14, 2011
    BibTeX
    @article{Theisen-Kunde,
       author = {Theisen-Kunde, D. and Tedsen, S. and Doehn, C. and Jocham, D. and Kausch von Schmeling, I.},
       title = {Comparison between a 1.92-mum fiber laser and a standard HF-dissection device for nephron-sparing kidney resection in a porcine in vivo study},
       journal = {Lasers Med Sci},
       volume = {26},
       number = {4},
       pages = {509-14},
       note = {Theisen-Kunde, Dirk
    Tedsen, Sonke
    Doehn, Christian
    Jocham, Dieter
    Kausch von Schmeling, Ingo
    England
    Lasers Med Sci. 2011 Jul;26(4):509-14. Epub 2011 Jan 18.},
       abstract = {Nephron-sparing surgery was performed in a porcine model with a 1.92-mum fiber laser dissection device in comparison to a standard high-frequency dissection device. In nine pigs, general anesthesia and a median laparotomy were performed to expose both kidneys. On six kidneys (three HF and three laser) a partial renal parenchyma resection of the lower pole without opening of the renal pelvis was performed (group A). On 12 kidneys (four HF and eight laser), a hemi nephrectomy with opening of the renal pelvis was performed (group B). Total resection time including hemostasis of the remaining tissue was 501 +/- 394 s in group "A-laser " vs. 176 +/- 139 s in group "A-HF". For the group "B", the total resection time was 1174 +/- 501 s (B laser) vs. 960 +/- 407 s (B-HF). Blood loss was 28 +/- 22 ml in group "A laser " vs. 15 +/- 15 ml in group "A-HF". In group "B", the blood loss was 98 +/- 73 ml (B laser) vs. 137 +/- 118 ml (B-HF). No ischemic time for the kidneys was needed in group "A" for both dissection devices. In group "B", ischemia of the kidneys was performed three times during the eight laser procedures (420 +/- 60 s) and only once at the four HF procedures (1,260 s). Healing process was observed over 4-6 weeks, survival rate was 100%, and no renal fistulas were found after the survival period. In conclusion, no significant differences were found between the compared dissection devices. However, the laser system with the flexible transmission fiber may have an advantage for a laparoscopic approach by steerable instruments.},
       keywords = {Animals
    Carcinoma, Renal Cell/*surgery
    Hemostasis
    Kidney/*surgery
    Kidney Neoplasms/*surgery
    Laser Therapy/*instrumentation/methods
    Swine},
       year = {2011}
    }
  • Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon: In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence. Journal of Biomedical Optics, no. 16, pp. 116025, 2011
    BibTeX
    @article{Orzekowsky2011,
       author = {Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon},
       title = {In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence},
       journal = {Journal of Biomedical Optics},
       volume = {16},
       number = {11},
       pages = {116025},
       keywords = {biological organs
    biomedical optical imaging
    cellular biophysics
    fluorescence
    laser applications in medicine
    two-photon processes},
       year = {2011}
    }
  • Fritz, Andreas and Zegelin, Andrea and Ptaszynski, Lars and Birngruber, Reginald and Brinkmann, Ralf: Dynamics of laser induced micro bubble clusters on tissue phantoms. no. 7885, pp. 78850S-78850S-6,
    BibTeX
    @inproceedings{Fritz2011,
       author = {Fritz, Andreas and Zegelin, Andrea and Ptaszynski, Lars and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Dynamics of laser induced micro bubble clusters on tissue phantoms},
       volume = {7885},
       pages = {78850S-78850S-6},
       note = {10.1117/12.875031},
       abstract = {Selective retina treatment (SRT) is a laser based method to treat retinal diseases associated with disorders of the retinal pigment epithelium (RPE) while preserving photoreceptors and choroid. Applying microsecond laser pulses to the 100- 200 strongly absorbing melanin granules inside the RPE cells induces transient micro bubbles which disrupt the cells. Aim of this work is to understand bubble dynamics in clusters with respect to the influence of the adjacent retina. Bubble dynamics were investigated in vitro on porcine RPE. An about 200 μm thick layer of agarose gel was applied to the RPE layer in order to simulate the mechanical properties of retina. Different laser pulse durations from 1 ns (532 nm, Nd:YAG) to 1.7 μs (527 nm, Nd:YLF) were used. The bubbles were investigated interferometrically (fiber interferometer @ 830 nm) and with fast flash photography (25 ns flash duration). Bubble lifetimes were measured. The results show that with retina phantoms the bubble formation threshold was reached at 2.5 times higher irradiation than without retina phantom for 1.7 μs laser pulses. The microbubbles generated with 1 ns laser pulses were almost not influenced by the agarose layer. Irradiation twofold over bubble formation threshold resulted in 3.5 times longer bubble lifetimes for μs and 2 times longer for ns pulse durations, respectively.},
       url = {http://dx.doi.org/10.1117/12.875031},
       type = {Conference Proceedings}
    }
    
  • Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Luft, Susanne and Baade, Alex and Bever, Marco and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf: Optoacoustic temperature determination and automatic coagulation control in rabbits. in Ophthalmic Technologies XXI, no. 7885, Proc. SPIE,
    BibTeX Link
    @inproceedings{Schlott2011,
       author = {Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Luft, Susanne and Baade, Alex and Bever, Marco and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Optoacoustic temperature determination and automatic coagulation control in rabbits},
       booktitle = {Ophthalmic Technologies XXI },
       editor = {Ho, Fabrice Manns; Per G. Söderberg; Arthur},
       publisher = {Proc. SPIE},
       volume = {7885},
       note = {10.1117/12.875104},
       abstract = {Retinal laser photocoagulation is an established treatment method for many retinal diseases like macula edema or diabetic retinopathy. The selection of the laser parameters is so far based on post treatment evaluation of the lesion size and strength. Due to local pigment variations in the fundus and individual transmission the same laser parameters often lead to an overtreatment. Optoacoustic allows a non invasive monitoring of the retinal temperature increase during retinal laser irradiation by measuring the temperature dependent pressure amplitudes, which are induced by short probe laser pulses. A 75 ns/ 523 nm Nd:YLF was used as a probe laser at a repetition rate of 1 kHz, and a cw / 532 nm treatment laser for heating. A contact lens was modified with a ring-shaped ultrasonic transducer to detect the pressure waves at the cornea. Temperatures were collected for irradiations leading to soft or invisible lesions. Based on this data the threshold for denaturation was found. By analyzing the initial temperature increase, the further temperature development during irradiation could be predicted. An algorithm was found to calculate the irradiation time, which is needed for a soft lesion formation, from the temperature curve. By this it was possible to provide a real-time dosimetry by automatically switching off the treatment laser after the calculated irradiation time. Automatically controlled coagulations appear softer and more uniformly.},
       keywords = {AutoPhoN},
       url = {http://dx.doi.org/10.1117/12.875104},
       type = {Conference Proceedings}
    }
    
  • Vogel, A and Venugopalan, V.: Pulsed laser ablation of tissue. Springer, Heidelberg, New York, 2011
    BibTeX
    @book{Vogel,
       author = {Vogel, A and Venugopalan, V.},
       title = {Pulsed laser ablation of tissue},
       publisher = {Springer, Heidelberg, New York},
       edition = {2},
       year = {2011}
    }
  • Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi: A Case of Retinal Injury By A Violet Light-Emitting Diode. Retinal Cases and Brief Reports, no. 5, pp. 223-226 10.1097/ICB.0b013e3181e180d5, 2011
    BibTeX
    @article{Obana,
       author = {Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi},
       title = {A Case of Retinal Injury By A Violet Light-Emitting Diode},
       journal = {Retinal Cases and Brief Reports},
       volume = {5},
       number = {3},
       pages = {223-226 10.1097/ICB.0b013e3181e180d5},
       abstract = {Purpose: To describe the first case of retinal injury by a misuse of a toy using light-emitting diode. Methods: A 15-year-old male Japanese student received irradiation on his right eye by a 5 mW light-emitting diode of 410 nm wavelength for 20 seconds in 2 days. He noticed decreased vision and central scotoma approximately 2 weeks later from these events. The mechanism of injury was evaluated from the estimated irradiance on the retina by comparison with experimental threshold data published. Results: Chorioretinal atrophy with visual loss and central scotoma has remained on the fovea. The patient received an estimated dose of 1.58 J/cm2 2 times, which was close to the experimentally determined radiant exposure for photochemical injury of rat retina. Conclusion: The violet light from light-emitting diodes is a potential hazard for the retina, and thus, direct viewing into the beam should be avoided. Children, especially, should not be allowed to play with such toys without being carefully instructed about their proper use and fully supervised.},
       keywords = {black light
    light-emitting diode
    photochemical damage
    retinal injury
    visual disturbance.
    01271216-201100530-00011},
       year = {2011}
    }
    
  • Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.: Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy. Laser Physics, no. 21, pp. 130-136, 2011
    BibTeX
    @article{Wang,
       author = {Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.},
       title = {Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy},
       journal = {Laser Physics},
       volume = {21},
       number = {1},
       pages = {130-136},
       keywords = {Physik und Astronomie},
       year = {2011}
    }
  • Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A: Extended coherence length Fourier domain mode locked lasers at 1310 nm. Optics express, no. 19, pp. 20930-9, OSA, oct,, 2011
    BibTeX Link
    @Article{HU_2011_Adler_a,
      Title                    = {{Extended coherence length Fourier domain mode locked lasers at 1310 nm}},
      Author                   = {Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {20930--9},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.020930},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=oe-19-21-20930}
    }
  • Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A: Extended focus high-speed swept source OCT with self-reconstructive illumination. Optics express, no. 19, pp. 12141-55, jun,, 2011
    BibTeX Link
    
    
    @Article{HU_2011_Blatter_a,
      Title                    = {{Extended focus high-speed swept source OCT with self-reconstructive illumination}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = jun,
      Number                   = {13},
      Pages                    = {12141--55},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.012141},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-13-12141}
    }
  • Klein, T and Wieser, W and Biedermann, B R and Eigenwillig, C M and Huber, R: FDML laser for megahertz retinal OCT imaging. in 2011 Conference on Lasers and Electro-Optics (CLEO), IEEE, 2011
    BibTeX
    @InProceedings{HU_2011_Klein_b,
      Title                    = {{FDML laser for megahertz retinal OCT imaging}},
      Author                   = {Klein, T and Wieser, W and Biedermann, B R and Eigenwillig, C M and Huber, R},
      Booktitle                = {2011 Conference on Lasers and Electro-Optics (CLEO)},
      Year                     = {2011},
      Publisher                = {IEEE}
    }
  • Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A: High-speed functional OCT with self-reconstructive Bessel illumination at 1300 nm. in European Conferences on Biomedical Optics, pp. 809104-809104-6, International Society for Optics and Photonics, jun,, 2011
    BibTeX Link
    @InProceedings{HU_2011_Blatter_b,
      Title                    = {{High-speed functional OCT with self-reconstructive Bessel illumination at 1300 nm}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2011},
      Editor                   = {Leitgeb, Rainer A and Bouma, Brett E},
      Month                    = jun,
      Pages                    = {809104--809104--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.889669},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1272395}
    }
  • Todor, Sebastian and Biedermann, Benjamin and Wieser, Wolfgang and Huber, Robert and Jirauschek, Christian: Instantaneous lineshape analysis of Fourier domain mode-locked lasers. Optics express, no. 19, pp. 8802-7, apr,, 2011
    BibTeX Link
    @Article{HU_2011_Todor_a,
      Title                    = {{Instantaneous lineshape analysis of Fourier domain mode-locked lasers}},
      Author                   = {Todor, Sebastian and Biedermann, Benjamin and Wieser, Wolfgang and Huber, Robert and Jirauschek, Christian},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = apr,
      Number                   = {9},
      Pages                    = {8802--7},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.008802},
      Institution              = {Institute for Nanoelectronics, Technische Universit\"{a}t M\"{u}nchen, Munich, Germany. todor@tum.de},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-9-8802\&seq=0}
    }
  • Klein, Thomas and Wieser, Wolfgang and Eigenwillig, Christoph M and Biedermann, Benjamin R and Huber, Robert: Megahertz OCT for ultrawide-field retinal imaging with a 1050 nm Fourier domain mode-locked laser. Optics express, no. 19, pp. 3044-62, feb,, 2011
    BibTeX Link
    @Article{HU_2011_Klein_a,
      Title                    = {{Megahertz OCT for ultrawide-field retinal imaging with a 1050 nm Fourier domain mode-locked laser}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Eigenwillig, Christoph M and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = feb,
      Number                   = {4},
      Pages                    = {3044--62},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.003044},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-4-3044}
    }
  • Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer A: Structural and functional imaging with extended focus dark-field OCT at 1300nm. in SPIE BiOS, pp. 78891D-78891D-6, International Society for Optics and Photonics, feb,, 2011
    BibTeX Link
    @InProceedings{HU_2011_Blatter_c,
      Title                    = {{Structural and functional imaging with extended focus dark-field OCT at 1300nm}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2011},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {78891D--78891D--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.875594},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=732654}
    }
  • Eigenwillig, Christoph M and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert: Wavelength swept amplified spontaneous emission source for high speed retinal optical coherence tomography at 1060 nm. Journal of biophotonics, no. 4, pp. 552-8, aug,, 2011
    BibTeX Link
    @Article{HU_2011_Eigenwillig_a,
      Title                    = {{Wavelength swept amplified spontaneous emission source for high speed retinal optical coherence tomography at 1060 nm}},
      Author                   = {Eigenwillig, Christoph M and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Journal of biophotonics},
      Year                     = {2011},
    
      Month                    = aug,
      Number                   = {7-8},
      Pages                    = {552--8},
      Volume                   = {4},
    
      Doi                      = {10.1002/jbio.201000104},
      ISSN                     = {1864-0648},
      Url                      = {http://onlinelibrary.wiley.com/doi/10.1002/jbio.201000104/abstract}
    }

2010

  • Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s. Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432., 2010
    BibTeX
    @book{Bonin2010,
       author = {Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s},
       publisher = {Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432.},
       abstract = {In vivo full-field (FF) optical coherence tomography (OCT) images of human retina are presented by using a rapidly tunable laser source in combination with an ultra-high-speed camera. Fourier-domain FF-OCT provided a way to increase the speed of retinal imaging by parallel acquisition of A-scans. Reduced contrast caused by cross talk was observed only below the retinal pigment epithelium. With a 100Hz sweep rate, FF-OCT was fast enough to acquire OCT images with acceptable motion artifacts. FF-OCT allows ultrafast retinal imaging, boosting image speed by a lack of moving parts and a considerably higher irradiation power.},
      year={2010}
    }
  • Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S: Photodynamic therapy to treat ovarian cancer. no. 5, National Cancer Institute (NCI) Alliance for Nanotechnology, 2010
    BibTeX
    @misc{Hasan,
       author = {Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S},
       title = {Photodynamic therapy to treat ovarian cancer},
       publisher = {National Cancer Institute (NCI) Alliance for Nanotechnology},
       volume = {5},
       number = {4},
       year = {2010}
    }
  • Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.: Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer. Cancer Res, no. 70, pp. 9234-42, 2010
    BibTeX
    @article{Rahmanzadeh2010,
       author = {Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.},
       title = {Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer},
       journal = {Cancer Res},
       volume = {70},
       number = {22},
       pages = {9234-42},
       note = {Using Smart Source Parsing
    Nov 15; Epub 2010 Nov 2},
       abstract = {Targeting molecular markers and pathways implicated in cancer cell growth is a promising avenue for developing effective therapies. Although the Ki-67 protein (pKi-67) is a key marker associated with aggressively proliferating cancer cells and poor prognosis, its full potential as a therapeutic target has never before been successfully shown. In this regard, its nuclear localization presents a major hurdle because of the need for intracellular and intranuclear delivery of targeting and therapeutic moieties. Using a liposomally encapsulated construct, we show for the first time the specific delivery of a Ki-67-directed antibody and subsequent light-triggered death in the human ovarian cancer cell line OVCAR-5. Photoimmunoconjugate-encapsulating liposomes (PICEL) were constructed from anti-pKi-67 antibodies conjugated to fluorescein 5(6)-isothiocyanate, as a photoactivatable agent, followed by encapsulation in noncationic liposomes. Nucleolar localization of the PICELs was confirmed by confocal imaging. Photodynamic activation with PICELs specifically killed pKi-67-positive cancer cells both in monolayer and in three-dimensional (3D) cultures of OVCAR-5 cells, with the antibody TuBB-9 targeting a physiologically active form of pKi-67 but not with MIB-1, directed to a different epitope. This is the first demonstration of (a) the exploitation of Ki-67 as a molecular target for therapy and (b) specific delivery of an antibody to the nucleolus in monolayer cancer cells and in an in vitro 3D model system. In view of the ubiquity of pKi-67 in proliferating cells in cancer and the specificity of targeting in 3D multicellular acini, these findings are promising and the approach merits further investigation.},
       year = {2010}
    }
  • Miura, Y and Klettner, A and Roider, J: VEGF antagonists decrease barrier function of retinal pigment epithelium in vitro: possible participation of intracellular glutathione. Invest Ophthalmol Vis Sci, no. 51, pp. 4848-55, 2010
    BibTeX Link
    @article{Miura2010,
       author = {Miura, Y and Klettner, A and Roider, J},
       title = {VEGF antagonists decrease barrier function of retinal pigment epithelium in vitro: possible participation of intracellular glutathione},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {51},
       number = {9},
       pages = {4848-55},
       note = {Miura, Yoko
    Klettner, Alexa
    Roider, Johann
    United States
    Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4848-55. Epub 2010 Apr 30.},
       abstract = {PURPOSE: To investigate the influence of VEGF antagonists on the barrier function of the retinal pigment epithelium and underlying mechanisms. METHODS: Porcine RPE cells were cultured on six-well membrane inserts. The cells were exposed to bevacizumab (62.5 microg/mL) or ranibizumab (25 microg/mL) for 24 hours (short term) or 9 days (long term). Transepithelial flux of FITC-dextran and intracellular levels of reduced glutathione (GSH) at normal and low-glucose conditions were investigated at different points in time. The influence of the addition of triamcinolone acetonide (TA) was investigated. The effect of GSH depletion on RPE permeability was examined using L-buthionine sulfoximine (BSO), a gamma-glutamylcysteine synthethase inhibitor. RESULTS: After short-term exposure, VEGF antagonists increased the transepithelial flux of FITC-dextran significantly on day 2. Bevacizumab, but not ranibizumab, increased permeability up to 9 days. Under long-term exposure, both drugs enhanced permeability for 7 days; bevacizumab had the stronger effect. The addition of TA inhibited this increase. At the ninth day of short- and long-term exposure, bevacizumab-exposed cells, but not ranibizumab-exposed cells, exhibited a significantly lower GSH level. In the low-glucose condition, both drugs accelerated the decrease of intracellular GSH for the first 48 hours. GSH depletion increased the permeability of retinal pigment epithelium. TA had no effect on BSO-induced GSH depletion. CONCLUSIONS: The results suggest that bevacizumab and ranibizumab may decrease RPE barrier function, with bevacizumab exhibiting a prolonged and more profound effect. Combination with TA is thought to be beneficial because of its protective effect on stabilizing RPE junctional integrity.},
       keywords = {Angiogenesis Inhibitors/ pharmacology
    Animals
    Antibodies, Monoclonal/ pharmacology
    Antibodies, Monoclonal, Humanized
    Buthionine Sulfoximine/pharmacology
    Cell Membrane Permeability/drug effects
    Cells, Cultured
    Dextrans/pharmacokinetics
    Enzyme Inhibitors/pharmacology
    Fluorescein-5-isothiocyanate/analogs & derivatives/pharmacokinetics
    Glucose/pharmacology
    Glutathione/ metabolism
    Retinal Pigment Epithelium/cytology/ drug effects/ metabolism
    Swine
    Tight Junctions/drug effects/metabolism
    Vascular Endothelial Growth Factor A/ antagonists & inhibitors/metabolis
    AutoPhoN},
       ISSN = {1552-5783 (Electronic)
    0146-0404 (Linking)},
       DOI = {10.1167/iovs.09-4699},
       year = {2010},
       type = {Journal Article}
    }
    
  • Framme, C. and Panagakis, G. and Birngruber, R.: Effects on Choroidal Neovascularization after Anti-VEGF Upload Using Intravitreal Ranibizumab, as Determined by Spectral Domain-Optical Coherence Tomography. Investigative Ophthalmology & Visual Science, no. 51, pp. 1671-1676, 2010
    BibTeX Link
    @article{Framme2010,
       author = {Framme, C. and Panagakis, G. and Birngruber, R.},
       title = {Effects on Choroidal Neovascularization after Anti-VEGF Upload Using Intravitreal Ranibizumab, as Determined by Spectral Domain-Optical Coherence Tomography},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {51},
       number = {3},
       pages = {1671-1676},
       note = {563VW
    Times Cited:18
    Cited References Count:17},
       abstract = {PURPOSE. It is unclear whether anti-VEGF monotherapy in age-related macular degeneration (AMD) achieves morphologic CNV regression or only stops further CNV growth. In this study, spectral domain-optical coherence tomography (SD-OCT) was used to image CNV structure before and after anti-VEGF treatment.
    METHODS. Out of 107 consecutive patients, a prospective CNV evaluation was possible in 78 of them. Newly diagnosed CNV (classic CNV: n = 16; occult CNV: n = 54; minimal classic CNV: n = 8) due to AMD was imaged before and 4 weeks after anti-VEGF upload in three intravitreal injections of ranibizumab. Qualitative (structural changes) and quantitative measurements (diameter and thickness) of the CNV were obtained from the OCT images.
    RESULTS. Classic CNV components were observed above the RPE/photoreceptor complex, whereas occult CNVs stayed below. Of all postoperative OCTs, 59% revealed complete dry retinal structures, 27% showed reduced edema, and 14% showed edema remaining unchanged. Mean macular thickness decreased significantly from 427 to 303 mu m (P = 0.000). Qualitatively, overall CNV architecture appeared to be unchanged in 78%, was reduced in thickness in 18%, and became larger in 4%. Quantitatively, in all CNV subtypes, the diameter of the CNV lesions (preoperative, 2813 mu m; postoperative, 2804 mu m) did not change after treatment (classic CNV: P = 0.390; occult CNV: P = 0.405, minimal classic CNV: P = 0.092) independent of postoperative retinal edema. The overall thickness of the lesion, however, was reduced from 205 to 175 mu m (P = 0.000). Thickness reduction was significantly enhanced especially in CNV with classic components (n = 24; 252 to 197 mu m; P = 0.000; reduction, 22%), whereas reduction was smaller but also significant in occult CNV (183 to 164 mu m; P = 0.003; reduction, 10%).
    CONCLUSIONS. With SD-OCT, CNV size can be two-dimensionally determined and followed up after intravitreal anti-VEGF treatment. In only 4% of CNV was enlargement observed, whereas in 78%, CNV architecture appeared qualitatively unchanged, independent of retinal edema. Quantitative measurements underlined stable CNV diameters for all subtypes but revealed significant reduction of thickness especially for classic CNV components. In this series, ranibizumab monotherapy was able to morphologically stop further CNV growth but, in most patients, did not lead to a major regression of CNV, especially of its occult components. (Invest Ophthalmol Vis Sci. 2010;51:1671-1676) DOI:10.1167/iovs.09-4496},
       keywords = {macular degeneration
    therapy
    verteporfin
    oct},
       ISSN = {0146-0404},
       DOI = {Doi 10.1167/Iovs.09-4496},
       url = {<Go to ISI>://WOS:000275164300060},
       year = {2010},
       type = {Journal Article}
    }
    
  • Bombien, R. and Lesche, C. and Lozonschi, L. and Feucker, M. and Brinkmann, R. and Dahmen, C. and Schunke, M. and Cremer, J. and Lutter, G.: Percutaneous Aortic Valve Replacement: Emerging Tractability for Sufficient Intracardiac Resection of the Aortic Valve. Innovations (Phila), no. 5, pp. 55-59, 2010
    BibTeX
    @article{Bombien,
       author = {Bombien, R. and Lesche, C. and Lozonschi, L. and Feucker, M. and Brinkmann, R. and Dahmen, C. and Schunke, M. and Cremer, J. and Lutter, G.},
       title = {Percutaneous Aortic Valve Replacement: Emerging Tractability for Sufficient Intracardiac Resection of the Aortic Valve},
       journal = {Innovations (Phila)},
       volume = {5},
       number = {1},
       pages = {55-59},
       note = {Philadelphia, Pa.
    Innovations (Phila). 2010 Jan;5(1):55-59.},
       abstract = {OBJECTIVE:: The feasibility of endovascular resection of highly calcified aortic valves has already been demonstrated by our group. Different endovascular and intracardiac tractability methods were applied. In this study, these technologies were analyzed comparing the tractability, the resection time, and the lesions in the surrounding tissue. METHODS:: All aortic valve resections (seven human hearts and 21 porcine hearts) were performed using a Thulium:YAG laser (continuous wave, wavelength of 2.01 mum, 20 watts power rating). In the first resection system, the laser fiber was controlled by a free in-lying flexible endoscope (O 2.5 mm, length of 600 mm). The distal part of the endoscope (40 mm) was moved in one plane by proximal manual control (three degrees of freedom). The resection system was separated into defined rooms assigning one room for one tool. The fiber was controlled by the above-mentioned endoscope (*) (three degrees of freedom). The third resection system was a mechanical microactuator carrying the laser fiber (three degrees of freedom). The fourth resection system contains a rotatable inlay with defined rooms and a newly designed nitinol (NiTi) microactuator that controlled the laser fiber (four degrees of freedom). The resection time per leaflet was measured in minutes. Gross anatomy and histology in the surrounding tissue were evaluated. RESULTS:: The resection time in approaches 1, 2, 3, and 4 was 5.5 +/- 2.3 minutes, 7.4 +/- 2.7 minutes, +/- 6.6 minutes, and2.3 +/- 1.2 minutes, respectively. The gross anatomy and histology of collateral damages revealed only superficial lesions of the surrounding tissue. The amount of lesions and the resection time were lower in the fourth approach with four degrees of freedom. CONCLUSIONS:: This analysis demonstrated that a precise tractability with four degrees of freedom is necessary for a faster and safer endovascular resection of the aortic valve. The analysis will help to optimize the ongoing development of the endovascular and intracardiac resection technology.},
       year = {2010}
    }
  • Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon: Optical coherence tomography with online visualization of more than seven rendered volumes per second. Journal of Biomedical Optics, no. 15, pp. 026014, 2010
    BibTeX
    @article{Probst2010,
       author = {Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon},
       title = {Optical coherence tomography with online visualization of more than seven rendered volumes per second},
       journal = {Journal of Biomedical Optics},
       volume = {15},
       number = {2},
       pages = {026014},
       keywords = {image resolution
    medical image processing
    optical tomography
    rendering (computer graphics)
    surgery},
       year = {2010}
    }
  • Orzekowsky-Schroeder, Regina and Klinger, Antje and Schuth, Anna and Freidank, Sebastian and Huttmann, Gereon and Gebert, Andreas and Vogel, Alfred: Intravital real-time study of tissue response to controlled laser-induced cavitation using 500-ps UV laser pulses focused in murine gut mucosa under online dosimetry and spectrally resolved 2-photon microscopy. no. 7568, pp. 756815, SPIE,
    BibTeX
    @inproceedings{Orzekowsky2010,
       author = {Orzekowsky-Schroeder, Regina and Klinger, Antje and Schuth, Anna and Freidank, Sebastian and Huttmann, Gereon and Gebert, Andreas and Vogel, Alfred},
       title = {Intravital real-time study of tissue response to controlled laser-induced cavitation using 500-ps UV laser pulses focused in murine gut mucosa under online dosimetry and spectrally resolved 2-photon microscopy},
       editor = {Daniel, L. Farkas and Dan, V. Nicolau and Robert, C. Leif},
       publisher = {SPIE},
       volume = {7568},
       pages = {756815},
    
    }
  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX Link
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {1423-0259
    Miura, Yoko
    Klettner, Alexa
    Noelle, Bernhard
    Hasselbach, Heike
    Roider, Johann
    Journal Article
    Switzerland
    Ophthalmic Res. 2010;43(3):122-33. doi: 10.1159/000252979. Epub 2009 Oct 29.},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       keywords = {Animals
    *Apoptosis
    Bruch Membrane/pathology
    Cell Survival
    Choroid/metabolism/*pathology/surgery
    Collagen Type IV/metabolism
    Enzyme-Linked Immunosorbent Assay
    Fluoresceins/metabolism
    Immunoenzyme Techniques
    In Situ Nick-End Labeling
    Laser Therapy
    Membrane Proteins/metabolism
    Occludin
    Organ Culture Techniques
    Retinal Pigment Epithelium/metabolism/*pathology/surgery
    Swine
    Time Factors
    Vascular Endothelial Growth Factor A/metabolism
    Wound Healing
    von Willebrand Factor/metabolism},
       ISSN = {0030-3747},
       DOI = {10.1159/000252979},
       year = {2010},
       type = {Journal Article}
    }
    
  • Mueller, M. and Schulz-Wackerbarth, C. and Steven, P. and Lankenau, E. and Bonin, T. and Mueller, H. and Brueggemann, A. and Birngruber, R. and Grisanti, S. and Huettmann, G.: Slit-lamp-adapted fourier-domain OCT for anterior and posterior segments: preliminary results and comparison to time-domain OCT. Curr Eye Res, no. 35, pp. 722-32, 2010
    BibTeX
    @article{Müller,
       author = {Mueller, M. and Schulz-Wackerbarth, C. and Steven, P. and Lankenau, E. and Bonin, T. and Mueller, H. and Brueggemann, A. and Birngruber, R. and Grisanti, S. and Huettmann, G.},
       title = {Slit-lamp-adapted fourier-domain OCT for anterior and posterior segments: preliminary results and comparison to time-domain OCT},
       journal = {Curr Eye Res},
       volume = {35},
       number = {8},
       pages = {722-32},
       note = {Using Smart Source Parsing
    Aug},
       abstract = {PURPOSE: To evaluate the diagnostic potential of a slit-lamp (SL)-adapted Fourier-domain (= spectral radar, SR) optical coherence tomography (OCT)-SL-SR-OCT-instrument as an in vivo imaging device for use in examinations of the anterior and posterior segments. MATERIALS AND METHODS: In a pilot study, 88 eyes from 70 healthy volunteers and patients were examined using a prototype Fourier-domain SL-SR-OCT system. Results were compared to those from the following commercially available systems: the 1310-nm SL-OCT (Heidelberg Engineering, Heidelberg, Germany) for anterior segment and the Stratus OCT (Zeiss Meditec, Jena, Germany) for posterior segment imaging. Our SL-SR-OCT provides 1025 axial scans, 5000 Hz line-scan frequency, scan length of up to 8 mm, axial depth in air of 3.5 mm, and resolution of 9 mum. For posterior visualization, a hand-held 78-diopter ophthalmoscopic lens was used. RESULTS: Our SL-SR-OCT system allowed simultaneous scanning with direct biomicroscopic and SL imaging of anterior and posterior segment structures. Anatomical structures and pathological changes were displayed with high resolution and excellent contrast. Measurements of corneal and retinal thickness were possible. In comparison to images obtained by the SL-OCT, our SL-SR-OCT boasted a higher resolution, thus providing more clinically relevant details of the corneal epithelium, internal structure of filtering blebs, etc. Complete imaging of the chamber angle was limited, however, due to the backscattering properties of the sclera at 830 nm. For posterior segment imaging, excellent delineation of the macula and optic nerve head details, with a distinct portrayal of macular pathology and retinal edema, was possible with SL-SR-OCT. CONCLUSION: SL-SR-OCT enables detailed imaging of physiological and pathological anterior and posterior segment structures. As a multi-purpose device, it offers a wide spectrum of applications, with high-quality OCT-imaging, in a comfortable setting without the need to move the patient.},
       year = {2010}
    }
  • Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R: Appearance of autofluorescence in RPE cells at the rim of photocoagulation. in FLIM 2010 - Symposium Fluorescence Lifetime Imaging of the Human Retina,
    BibTeX
    @inproceedings{Miura2010,
       author = {Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R},
       title = {Appearance of autofluorescence in RPE cells at the rim of photocoagulation},
       booktitle = {FLIM 2010 - Symposium "Fluorescence Lifetime Imaging of the Human Retina"},
       type = {Conference Proceedings}
    }
    
    
    
  • Xie, Y. and Bonin, T. and Loeffler, S. and Huettmann, G. and Tronnier, V. and Hofmann, U. G.: Fiber spectral domain optical coherence tomography for in vivo rat brain imaging. no. 7715, pp. 77152F, SPIE,
    BibTeX
    @inproceedings{Xie,
       author = {Xie, Y. and Bonin, T. and Loeffler, S. and Huettmann, G. and Tronnier, V. and Hofmann, U. G.},
       title = {Fiber spectral domain optical coherence tomography for in vivo rat brain imaging},
       editor = {Jurgen, Popp and Wolfgang, Drexler and Valery, V. Tuchin and Dennis, L. Matthews},
       publisher = {SPIE},
       volume = {7715},
       pages = {77152F},
    
    }
  • Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba: Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells. no. 7576, pp. 757602, SPIE,
    BibTeX
    @inproceedings{Rahmanzadeh,
       author = {Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba},
       title = {Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells},
       editor = {Samuel, Achilefu and Ramesh, Raghavachari},
       publisher = {SPIE},
       volume = {7576},
       pages = {757602},
    
    }
  • Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.: Optical coherence tomography for process control of laser micromachining. Rev Sci Instrum, no. 81, pp. 033705, 2010
    BibTeX
    @article{Wiesner2010,
       author = {Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.},
       title = {Optical coherence tomography for process control of laser micromachining},
       journal = {Rev Sci Instrum},
       volume = {81},
       number = {3},
       pages = {033705},
       note = {Using Smart Source Parsing
    Mar},
       abstract = {In situ surface imaging for nondestructive evaluation (NDE) by optical coherence tomography (OCT) before, during, and after ablative laser processing is presented. Furthermore, it is shown that the ability of in situ characterization is beneficial for samples such as optical fibers, which are difficult to handle in the standard analysis. Surface images taken by the OCT are compared with these common analysis tools such as scanning electron microscopy (SEM), reflected-light, and confocal microscopy. An axial resolution of approximately 126 nm for surface detection and a lateral resolution <2.5 microm are obtained and the potential of the setup to imaging structures with high aspect ratio is demonstrated.},
       year = {2010}
    }
  • Roider, J. and Liew, S. H. and Klatt, C. and Elsner, H. and Poerksen, E. and Hillenkamp, J. and Brinkmann, R. and Birngruber, R.: Selective retina therapy (SRT) for clinically significant diabetic macular edema. Graefes Arch Clin Exp Ophthalmol, no. 248, pp. 1263-72, 2010
    BibTeX
    @article{Roider,
       author = {Roider, J. and Liew, S. H. and Klatt, C. and Elsner, H. and Poerksen, E. and Hillenkamp, J. and Brinkmann, R. and Birngruber, R.},
       title = {Selective retina therapy (SRT) for clinically significant diabetic macular edema},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {248},
       number = {9},
       pages = {1263-72},
       note = {Using Smart Source Parsing
    Sep},
       abstract = {PURPOSE: To test selective retina therapy (SRT) as a treatment of clinically significant diabetic macular edema (DME). METHODS: Prospective two-center interventional uncontrolled phase II pilot study. Thirty-nine eyes of 39 patients with previously untreated non-ischemic DME were treated with focal laser treatment using a Q-switched frequency doubled Nd:YLF laser which selectively affects the retinal pigment epithelium while sparing the photoreceptor layer. Optoacoustic measurements, fundus fluorescein angiography (FFA), and funduscopy were used to determine the individual threshold of RPE damage of each patient. The pulse energy was adjusted to apply angiographically visible but funduscopically invisible effects. Optoacoustic measurements were correlated with funduscopy and FFA. Follow-up examinations at 3 and 6 months post-treatment included best-corrected ETDRS visual acuity (BCVA), FFA, fundus photography, and retinal thickness measured by optical coherence tomography. The primary outcome measure was change of BCVA. Other outcome measures were change of retinal thickness, presence of hard exudates, leakage in FFA, accuracy of optoacoustic measurements, and correlation of BCVA with change of anatomical and systemic parameters. RESULTS: Mean BCVA improved from 43.7 letters (standard deviation, SD=9.1) at baseline to 46.1 letters (SD=10.5) at the 6-month follow-up (p=0.02). BCVA improved (>5 letters) or remained stable (+/-5 letters) in 84% of eyes. Thirteen percent of eyes improved by > or =10 letters, while 16% of eyes lost more than 5 letters. There was no severe loss of vision (> or =15 letters). Overall, retinal thickness, hard exudates, and leakage in FFA did not change significantly (p> 0.05), while improvement of BCVA correlated with a reduction of hard exudates (p=0.01) and central retinal thickness (p=0.01). Specificity and sensitivity of detecting the angiographic visible threshold of RPE damage by optoacoustic measurements were 86% and 70% respectively. No adverse effects or pain were noted during or after treatment. Conclusions Functional and anatomical improvement or stabilization was observed in most patients. SRT appears to be safe. Optoacoustic measurements accurately detect the individual threshold of RPE damage. A randomized trial is required to further test efficacy and safety of SRT as a treatment of clinically significant diabetic macular edema (DME).},
       year = {2010}
    }
    
  • Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.: An optical coherence tomography study for imaging the round window niche and the promontorium tympani. no. 7548, pp. 754833, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.},
       title = {An optical coherence tomography study for imaging the round window niche and the promontorium tympani},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Reza, S. Malek and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7548},
       pages = {754833},
    
    }
  • Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.: An optical coherence tomography study for imaging the round window niche and the promontorium tympani. no. 7548, pp. 754833, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.},
       title = {An optical coherence tomography study for imaging the round window niche and the promontorium tympani},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Reza, S. Malek and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7548},
       pages = {754833},
    
    }
  • Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.: Development and applications of photo-triggered theranostic agents. Adv Drug Deliv Rev, no. 62, pp. 1094-124, 2010
    BibTeX
    @article{Rai,
       author = {Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.},
       title = {Development and applications of photo-triggered theranostic agents},
       journal = {Adv Drug Deliv Rev},
       volume = {62},
       number = {11},
       pages = {1094-124},
       note = {Rai, Prakash
    Mallidi, Srivalleesha
    Zheng, Xiang
    Rahmanzadeh, Ramtin
    Mir, Youssef
    Elrington, Stefan
    Khurshid, Ahmat
    Hasan, Tayyaba
    Nihms238162
    Adv Drug Deliv Rev. 2010 Aug 30;62(11):1094-124. Epub 2010 Sep 19.},
       abstract = {Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.},
       keywords = {Animals
    Anti-Infective Agents/diagnostic use/therapeutic use
    Antineoplastic Agents/diagnostic use/therapeutic use
    Diagnostic Imaging/ methods
    Drug Carriers/diagnostic use/therapeutic use
    Humans
    Infection/ diagnosis/ drug therapy
    Nanoparticles/diagnostic use/therapeutic use
    Neoplasms/ diagnosis/drug therapy/ therapy
    Phototherapy/ methods},
       year = {2010}
    }
    
    
  • Prahs, P. and Walter, A. and Regler, R. and Theisen-Kunde, D. and Birngruber, R. and Brinkmann, R. and Framme, C.: Selective retina therapy (SRT) in patients with geographic atrophy due to age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol, no. 248, pp. 651-8, 2010
    BibTeX Link
    @article{Prahs2010,
       author = {Prahs, P. and Walter, A. and Regler, R. and Theisen-Kunde, D. and Birngruber, R. and Brinkmann, R. and Framme, C.},
       title = {Selective retina therapy (SRT) in patients with geographic atrophy due to age-related macular degeneration},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {248},
       number = {5},
       pages = {651-8},
       note = {1435-702x
    Prahs, Philipp
    Walter, Andreas
    Regler, Roman
    Theisen-Kunde, Dirk
    Birngruber, Reginald
    Brinkmann, Ralf
    Framme, Carsten
    Journal Article
    Germany
    Graefes Arch Clin Exp Ophthalmol. 2010 May;248(5):651-8. doi: 10.1007/s00417-009-1208-1. Epub 2009 Dec 22.},
       abstract = {BACKGROUND: For geographic atrophy (GA) due to age-related macular degeneration (AMD) there is so far no approved treatment option. Usually, increased autofluorescence (AF) levels of different patterns adjacent to the atrophic area indicate lipofuscin-laden retinal pigment epithelium (RPE) cells at a high risk for apoptosis. Herein, SRT was used to selectively treat these cells to stimulate RPE proliferation, in order to reduce or ideally stop further growth of the atrophic area. MATERIAL AND METHODS: Six eyes of six patients with bilateral equally pronounced GA were treated by SRT, while the fellow eye served as control. Irradiation was performed using a prototype SRT laser (Medical Laser Center Lubeck, Nd:YLF laser; 527 nm; 200 ns/1.7 micros pulse duration; 30 repetitive pulses at 100 Hz). Test lesions with increasing energies were applied at the lower vessel arcade to determine the individual angiographic and ophthalmoscopic threshold radiant exposures. Treatment was then performed in the area of increased AF adjacent to the GA using energies between both thresholds. The GA progression rates of treated and fellow eyes were evaluated. RESULTS: After a 1-year follow-up, a progression of the atrophic area was observed in the treated eyes (0.7-8.0 mm(2)/yr, mean 3.0 mm(2)/yr; 46%/yr) whereas the progression rates of the fellow eyes were insignificantly lower (0.46-4.04 mm(2)/yr, mean 1.9 mm(2)/yr; 30%/yr; p = 0.134). The progression rate in the treated eyes of two patients increased significantly, while in the other four patients, the progression rates were nearly the same between both eyes. Moreover, one of these two eyes showed an unexpected RPE reaction after treatment, since all laser lesions led to RPE atrophy and thus an accelerated enlargement of the GA occurred. CONCLUSION: SRT in the hyperautofluorescent areas of GA was not able to stop or slow down the progression of GA. However, modified treatment strategies might be more promising, e.g. placing the spots outside the hyperautofluorescent areas where RPE apoptosis is postulated. Moreover, SRT studies on GA might be more successfully performed on specific subgroups of GA, based on autofluorescence and other findings.},
       keywords = {Aged
    Aged, 80 and over
    Disease Progression
    Fluorescein Angiography
    Fluorescence
    Follow-Up Studies
    Geographic Atrophy/etiology/physiopathology/*surgery
    Humans
    Image Processing, Computer-Assisted
    *Laser Coagulation
    Lasers, Solid-State/therapeutic use
    Lipofuscin/metabolism
    Macular Degeneration/complications/physiopathology/*surgery
    Ophthalmologic Surgical Procedures/*methods
    Pilot Projects
    Prognosis
    Retinal Pigment Epithelium/metabolism},
       ISSN = {0721-832x},
       DOI = {10.1007/s00417-009-1208-1},
       year = {2010},
       type = {Journal Article}
    }
    
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Direct measurement of the instantaneous linewidth of rapidly wavelength-swept lasers. Optics letters, no. 35, pp. 3733-5, nov,, 2010
    BibTeX Link
    @Article{HU_2010_Biedermann_a,
      Title                    = {{Direct measurement of the instantaneous linewidth of rapidly wavelength-swept lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Journal                  = {Optics letters},
      Year                     = {2010},
    
      Month                    = nov,
      Number                   = {22},
      Pages                    = {3733--5},
      Volume                   = {35},
    
      Doi                      = {10.1364/OL.35.003733},
      ISSN                     = {1539-4794},
      Url                      = {http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-35-22-3733}
    }
    
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: FDML swept source at 1060 nm using a tapered amplifier. in BiOS, pp. 75541H-75541H-5, International Society for Optics and Photonics, feb,, 2010
    BibTeX Link
    @InProceedings{HU_2010_Marschall_c,
      Title                    = {{FDML swept source at 1060 nm using a tapered amplifier}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Booktitle                = {BiOS},
      Year                     = {2010},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {75541H--75541H--5},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.842011},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1343844}
    }
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Hsu, Kevin and Hansen, Kim P and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: Fourier domain mode-locked swept source at 1050 nm based on a tapered amplifier. Optics express, no. 18, pp. 15820-31, jul,, 2010
    BibTeX Link
    @Article{HU_2010_Marschall_a,
      Title                    = {{Fourier domain mode-locked swept source at 1050 nm based on a tapered amplifier}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Hsu, Kevin and Hansen, Kim P and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Journal                  = {Optics express},
      Year                     = {2010},
    
      Month                    = jul,
      Number                   = {15},
      Pages                    = {15820--31},
      Volume                   = {18},
    
      Doi                      = {10.1364/OE.18.015820},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-15-15820}
    }
    
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: High-power FDML laser for swept source-OCT at 1060 nm. in SPIE Photonics Europe, pp. 77150B-77150B-5, International Society for Optics and Photonics, apr,, 2010
    BibTeX Link
    @InProceedings{HU_2010_Marschall_b,
      Title                    = {{High-power FDML laser for swept source-OCT at 1060 nm}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Booktitle                = {SPIE Photonics Europe},
      Year                     = {2010},
      Editor                   = {Popp, J\"{u}rgen and Drexler, Wolfgang and Tuchin, Valery V and Matthews, Dennis L},
      Month                    = apr,
      Pages                    = {77150B--77150B--5},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.854238},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1345306}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: High-Quality 3-D Imaging with Multimegahertz OCT. Optics and Photonics News, no. 21, pp. 28 - 29, OSA, dec,, 2010
    BibTeX Link
    @Article{HU_2010_Wieser_a,
      Title                    = {{High-Quality 3-D Imaging with Multimegahertz OCT}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics and Photonics News},
      Year                     = {2010},
    
      Month                    = dec,
      Number                   = {12},
      Pages                    = {28 -- 29 },
      Volume                   = {21},
    
      Doi                      = {10.1364/OPN.21.12.000028},
      ISSN                     = {1047-6938},
      Publisher                = {OSA},
      Url                      = {http://www.osa-opn.org/abstract.cfm?URI=opn-21-12-28 http://www.opticsinfobase.org/abstract.cfm?URI=OPN-21-12-28}
    }
  • Todor, Sebastian and Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert: Linewidth Optimization of Fourier Domain Mode-Locked Lasers. Conference on Lasers and Electro-Optics 2010, pp. CMW7 1-3, OSA, Washington, D.C., 2010
    BibTeX Link
    @Article{HU_2010_Todor_a,
      Title                    = {{Linewidth Optimization of Fourier Domain Mode-Locked Lasers}},
      Author                   = {Todor, Sebastian and Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert},
      Journal                  = {Conference on Lasers and Electro-Optics 2010},
      Year                     = {2010},
      Pages                    = {CMW7 1--3 },
    
      Address                  = {Washington, D.C.},
      Doi                      = {10.1364/CLEO.2010.CMW7},
      ISBN                     = {978-1-55752-889-6},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO-2010-CMW7}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second. Optics express, no. 18, pp. 14685-704, jul,, 2010
    BibTeX Link
    @Article{HU_2010_Wieser_b,
      Title                    = {{Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2010},
    
      Month                    = jul,
      Number                   = {14},
      Pages                    = {14685--704},
      Volume                   = {18},
    
      Doi                      = {10.1364/OE.18.014685},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen,Oettingenstr. 67, 80538 Munich, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-14-14685}
    }

2009

  • Bever, M. and Schlott, K. and Ptaszynski, L. and Koinzer, S. and Birngruber, R. and Brinkmann, R.: Automatische Dosimetrie bei der Laserphotokoagulation der Netzhaut. 3. Dresdner Medizintechnik-Symposium mit DFG Forschungsschwerpunkt Protektive Beatmungskonzepte, no. 10, 2009
    BibTeX
    @article{Bever2009,
       author = {Bever, M. and Schlott, K. and Ptaszynski, L. and Koinzer, S. and Birngruber, R. and Brinkmann, R.},
       title = {Automatische Dosimetrie bei der Laserphotokoagulation der Netzhaut},
       journal = {3. Dresdner Medizintechnik-Symposium mit DFG Forschungsschwerpunkt Protektive Beatmungskonzepte},
       volume = {10},
       year = {2009},
       type = {Journal Article}
    }
    
  • Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.: Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review. Klinische Monatsblatter Fur Augenheilkunde, no. 226, pp. 958-964, 2009
    BibTeX Link
    @article{Hüttmann2009,
       author = {Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.},
       title = {Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review},
       journal = {Klinische Monatsblatter Fur Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958-964},
       note = {542VT
    Times Cited:6
    Cited References Count:48},
       abstract = {Background: Optical coherence tomography (OCT) is new diagnostic procedure that has rapidly evolved in the last years. The recently developed spectral domain OCT allows one to increase the imaging speed by a hundred times compared to the first generation time domain OCT and enables three-dimensional imaging as well as real-time imaging of fast moving structures. Volumetric imaging improves the quantitative measurement of morphology and the evaluation of temporal changes. In addition, an exact correlation with images acquired with other imaging modalities is possible. Real-time imaging enables also the use of OCT during examinations with the slit-lamp and during ophthalmological surgery.
    Methods: A spectral domain OCT was adapted to a slit lamp. 70 patients (91 eyes) were examined at the anterior or posterior segment of the eye. Images of healthy structures and different pathologies were compared to OCT images obtained with Stratus 3, Spectralis, and the SL-OCT. To demonstrate the feasibility of OCT during surgery, spectral domain OCT devices working with 20000 and 210000 A scans per second were coupled by specially developed optics to the camera port of a surgical microscope. The device was tested with phantoms and enucleated pig eyes.
    Results and Conclusions: A5 kHz spectral domain OCT can image the retina during slit lamp-based indirect ophthalmoscopy with a quality similar to that of the Stratus 3. In addition, relevant structures of the anterior segment were imaged. Here, compared to commercially available devices, the imaged field was smaller and the angle of the anterior chamber was not directly visible due to the 830 nm wavelength, which had to be used for retinal imaging. Through the surgical microscope, a volumetric imaging of epithelium, Bowman's, Descemet's membranes, limbus, iris, lens, conjunctiva and sclera was demonstra-ted with several tens of centimetre working distance. Instruments and incisions in the cornea were visualised with 20 mu m precision. Real-time imaging and visualisation of volumetric OCT data were also demonstrated. In principle, all technical problems of an intraoperative use of OCT have been solved and a clinical trial will start in the near future. OCT has the potential to improve the precision of surgical interventions and may even enable new interventions.},
       keywords = {physiological optics
    cornea
    anatomy
    in-vivo
    ultrahigh-resolution
    anterior segment
    time-domain
    blood-flow
    eye
    surgery
    oct
    cyclophotocoagulation
    cornea},
       ISSN = {0023-2165},
       DOI = {DOI 10.1055/s-0028-1109939},
       url = {<Go to ISI>://WOS:000273527800005},
       year = {2009},
       type = {Journal Article}
    }
    
  • Framme, C. and Panagakis, G. and Birngruber, R.: Effects on Choroidal Neovascularizations after Anti-VEGF Upload Using Intravitreal Ranibizumab as Determined by Spectral Domain Optical Coherence Tomography. Invest Ophthalmol Vis Sci, 2009
    BibTeX Link
    @article{Framme2009,
       author = {Framme, C. and Panagakis, G. and Birngruber, R.},
       title = {Effects on Choroidal Neovascularizations after Anti-VEGF Upload Using Intravitreal Ranibizumab as Determined by Spectral Domain Optical Coherence Tomography},
       journal = {Invest Ophthalmol Vis Sci},
       note = {Journal article
    Investigative ophthalmology & visual science
    Invest Ophthalmol Vis Sci. 2009 Oct 29.},
       abstract = {Purpose: To image CNV structure before and after anti-VEGF treatment using spectral domain optical coherence tomography (SD-OCT). Material and methods: In 78 patients newly diagnosed CNVs (classic CNV: n=16; occult CNV: n=54; minimal classic CNV: n=8) due to AMD were imaged before and 4 weeks after anti-VEGF upload with 3 intravitreal injections of Ranibizumab. Qualitative (structural changes) and quantitative measurements (diameter and thickness) of the CNVs were obtained from the OCT images using the Heidelberg Eye Explorer software. Results: Classic CNV components were observed above the RPE/photoreceptor complex, whereas occult CNVs stayed below. Qualitatively, overall CNV architecture appeared to be unchanged in 78%, reduced in thickness in 18%, and became larger in 4%. Quantitatively, for all CNV subtypes the diameter of CNVs (preoperatively: 2813mum; postoperatively: 2804mum) did not change after treatment (classic CNVs: p=0.390; occult CNVs: p=0.405, minimal classic CNVs: p=0.092) independently of postoperative retinal edema. The overall thickness of the CNV; however, reduced from 205mum to 175mum (p=0,000). Thickness reduction was significantly enhanced especially in CNVs with classic components (n=24; 252mum to 197mum (p=0.000); reduction: 22%), whereas reduction was smaller but also significant in occult CNVs (183mum to 164mum (p=0.003); reduction: 10%). Conclusion: Using SD-OCT CNV size can be two-dimensionally determined and followed up after intravitreal anti-VEGF treatment. Quantitative measurements underlined stable CNV diameters for all subtypes but revealed significant reduction of thickness especially for classic CNV components. Thus, Ranibizumab monotherapy is able to morphologically stop further CNV growth but does not lead to a major regression of CNVs especially of its occult components.},
       ISSN = {1552-5783 (Electronic)
    0146-0404 (Linking)},
       DOI = {iovs.09-4496 [pii]
    10.1167/iovs.09-4496},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19875667},
       year = {2009},
       type = {Journal Article}
    }
    
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Optical coherence tomography of the oval window niche.. J Laryngol Oto, no. 1, pp. 1-6, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Optical coherence tomography of the oval window niche.},
       journal = { J Laryngol Oto},
       volume = {1},
       pages = {1-6},
       year = {2009}
    }
  • Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.: Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection. J Biomed Opt, no. 14, pp. 054034, 2009
    BibTeX
    @article{Yao,
       author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
       title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
       journal = {J Biomed Opt},
       volume = {14},
       pages = {054034},
       note = {Journal article},
       year = {2009}
    }
  • Tiede, S. and Koop, N. and Kloepper, J. E. and Fassler, R. and Paus, R.: Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells. Stem Cells, no. 27, pp. 2793-803, 2009
    BibTeX Link
    @article{Tiede2009,
       author = {Tiede, S. and Koop, N. and Kloepper, J. E. and Fassler, R. and Paus, R.},
       title = {Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells},
       journal = {Stem Cells},
       volume = {27},
       number = {11},
       pages = {2793-803},
       ISSN = {1066-5099},
       DOI = {10.1002/stem.213},
       year = {2009},
       type = {Journal Article}
    }
    
  • Hüttmann, G.: Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review. Klinische Monatsblätter für Augenheilkunde, no. 226, pp. 958, 2009
    BibTeX
    @article{Hüttmann2009,
       author = {Hüttmann, G.},
       title = {Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review},
       journal = {Klinische Monatsblätter für Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958},
       year = {2009}
    }
  • Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon: Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies. Journal of Biomedical Optics, no. 14, pp. 064040-064040, 2009
    BibTeX
    @article{Steven,
       author = {Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon},
       title = {Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies},
       journal = {Journal of Biomedical Optics},
       volume = {14},
       number = {6},
       pages = {064040-064040},
       note = {10.1117/1.3275475},
       abstract = {Minimally invasive imaging of ocular surface pathologies aims at securing clinical diagnosis without actual tissue probing. For this matter, confocal microscopy (Cornea Module) is in daily use in ophthalmic practice. Multiphoton microscopy is a new optical technique that enables high-resolution imaging and functional analysis of living tissues based on tissue autofluorescence. This study was set up to compare the potential of a multiphoton microscope (DermaInspect) to the Cornea Module. Ocular surface pathologies such as pterygia, papillomae, and nevi were investigated in vivo using the Cornea Module and imaged immediately after excision by DermaInspect. Two excitation wavelengths, fluorescence lifetime imaging and second-harmonic generation (SHG), were used to discriminate different tissue structures. Images were compared with the histopathological assessment of the samples. At wavelengths of 730nm, multiphoton microscopy exclusively revealed cellular structures. Collagen fibrils were specifically demonstrated by second-harmonic generation. Measurements of fluorescent lifetimes enabled the highly specific detection of goblet cells, erythrocytes, and nevus-cell clusters. At the settings used, DermaInspect reaches higher resolutions than the Cornea Module and obtains additional structural information. The parallel detection of multiphoton excited autofluorescence and confocal imaging could expand the possibilities of minimally invasive investigation of the ocular surface toward functional analysis at higher resolutions.},
       year = {2009}
    }
  • Lachmann, Kristina and Eckert, Sebastian and Vogel, Alfred and Klinger, Antje and Gebert, Andreas and Klages, Claus-Peter: Development of a multifunctional coating system for laser-induced material transport. Progress in Organic Coatings, no. 64, pp. 294-299, 2009
    BibTeX
    @article{Lachmann,
       author = {Lachmann, Kristina and Eckert, Sebastian and Vogel, Alfred and Klinger, Antje and Gebert, Andreas and Klages, Claus-Peter},
       title = {Development of a multifunctional coating system for laser-induced material transport},
       journal = {Progress in Organic Coatings},
       volume = {64},
       number = {2–3},
       pages = {294-299},
       abstract = {The aim of our research is to develop a novel surface coating for the use in laser microdissection and laser pressure catapulting (LMPC). LMPC is a contact- and contamination-free technique to separate histologic material and living cells for further proteomic and genomic analysis. Several physico-chemical functions must be included within the optimum coating system designed for this purpose, like optical absorption at the laser wavelength, combined with optical transparency in the visible region, a control of the laser ablation process, mechanical stability and biocompability for the adhesion of the histologic material. To achieve the optimum system the combination of several layers is required. The optical absorbance to capture the radiation energy from a frequency-tripled Nd:YAG laser (?&#xa0;=&#xa0;355&#xa0;nm) is reached by a thin layer of zinc oxide (ZnO), deposited by hollow cathode gas flow sputtering. The laser ablation process is controlled by a polyelectrolyte multilayer, consisting of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS). The evaporation of chemisorbed water from the film is used to promote the catapulting process. For the mechanically stable, laser-dissectible layer organic coatings, like photoresists or lacquers, are suitable. Silica-containing polyacrylate nanocomposites were employed for this purpose. The investigation of the coating system included LMPC experiments with varying compositions of the layer system. The best results were obtained using a system consisting of ZnO, a polyelectrolyte multilayer deposited from 0.1&#xa0;M Na2SO4 containing polymer solutions, and a 1.5-?m thick layer of the polyacrylate nanocomposite. To check the quality of the developed system, experiments with the commonly used poly(ethylene naphthalate) (PEN) foil were performed simultaneously. In addition to the determination of the parameters required for LMPC, quantitative real-time Polymerase Chain Reaction (rt-PCR) of the dissected material verified the benefit of the new system.},
       keywords = {Laser microdissection
    Laser pressure catapulting
    Polyelectrolyte multilayer
    Laser ablation
    Zinc oxide},
       year = {2009}
    }
  • Langejürgen, J and Schlott, K and Bever, M and Hausmann, K and Koinzer, S and Ptaszynski, L and Roider, J and Birngruber, R and Brinkmann, R: Dependence of optoacoustic transients on exciting laser parameters for real-time monitoring of retinal photocoagulation. pp. 73730K-73730K,
    BibTeX Link
    @inproceedings{Langejürgen2009,
       author = {Langejürgen, J and Schlott, K and Bever, M and Hausmann, K and Koinzer, S and Ptaszynski, L and Roider, J and Birngruber, R and Brinkmann, R},
       title = {Dependence of optoacoustic transients on exciting laser parameters for real-time monitoring of retinal photocoagulation},
       pages = {73730K-73730K},
       note = {10.1117/12.831913},
       abstract = {The extent of retinal laser coagulations depends on the temperature increase at the fundus and the time of irradiation. Due to light scattering within the eye and variable fundus pigmentation the induced temperature increase and therefore the extent of the coagulations cannot be predicted solely from the laser parameters. We use optoacoustics to monitor the temperature rise in real-time in vivo (rabbit) and ex vivo (porcine eye) and to automatically control the coagulation strength. Continuous wave treatment laser radiation and pulsed probe laser light (1-1100 ns) are coupled into the same fibre and are imaged onto the retina by a laser slit lamp. The temperature dependent pressure waves are detected by an ultrasonic transducer embedded in a customary contact lens. Below the coagulation threshold the increase in acoustic amplitude due to thermal tissue expansion is up to 40 %. Best signal to noise ratios &gt; 10 are achieved with probe pulse durations of 1 to 75 ns. Further a time critical algorithm is developed which automatically ceases laser treatment when a certain preset coagulation strength is achieved. Coagulations with similar extent are obtained with this method in vitro and in vivo even when varying the power of the treatment laser by 50 %. These preliminary results are very promising, thus this method might be suitable for an automatic feedback controlled photocoagulation with adjustable coagulation strength.},
    url = {http://dx.doi.org/10.1117/12.831913},
       type = {Conference Proceedings}
    }
    
  • Schlott, Kerstin and Langejürgen, Jens and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf: Time resolved detection of tissue denaturation during retinal photocoagulation. pp. 73730E-73730E,
    BibTeX Link
    @inproceedings{Schlott2009,
       author = {Schlott, Kerstin and Langejürgen, Jens and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Time resolved detection of tissue denaturation during retinal photocoagulation},
       editor = {7373, Proc. SPIE},
       pages = {73730E-73730E},
       note = {10.1117/12.831877},
       abstract = {The retinal photocoagulation is an established treatment method for different retinal diseases. The extent of the thermal coagulations depends strongly on the generated temperature increase. Until now the dosage is based on a pool of experience of the treating physicians as well as the appearance of the whitish lesions on the retina. The temperature course during photocoagulation can be measured in real-time by optoacoustics. A frequency-doubled Q-switched Nd:YLF laser (523nm, 75 ns) is used for optoacoustic excitation and a continuous-wave Nd:YAG laser (532nm) with adjustable irradiation time and power for heating of the fundus tissue. The onset of coagulation is determined by a photodiode that is placed directly behind enucleated porcine eyes, which served as a model. The onset of coagulation is observed clearly when scattering sets in. The required power for coagulation increases exponentially with decreasing irradiation time. The first results on rabbit eyes in vivo indicate that the onset of coagulation defined by just barely visibile lesions at a slit lamp sets in at an ED50 threshold temperature of 63°C for an irradiation time of 400 ms. In conclusion, optoacoustics can be used to determine temperatures during retinal laser treatments in real-time. This allows evaluating the time-temperature-dependence of retinal coagulation in vivo.},
       keywords = {AutoPhoN},
       url = {http://dx.doi.org/10.1117/12.831877},
       type = {Conference Proceedings}
    }
    
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Intra-operative application of optical coherence tomography with an operating microscope.. J Laryngol Otol, no. 123, pp. 1027-1030, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Intra-operative application of optical coherence tomography with an operating microscope.},
       journal = {J Laryngol Otol},
       volume = {123},
       pages = {1027-1030},
       year = {2009}
    }
  • Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.: Optische Kohärenztomographie in der Mittelohrchirurgie. HNO, no. 57, pp. 421-427, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.},
       title = {Optische Kohärenztomographie in der Mittelohrchirurgie},
       journal = {HNO},
       volume = {57},
       number = {5},
       pages = {421-427},
       abstract = {Die Anwendung der optischen Kohärenztomographie (OCT) in der Mittelohrchirurgie gilt als vielversprechendes Diagnostikum, insbesondere wenn es um die intraoperative Abklärung der Ursache einer Stapesfixation, die intraoperative Beurteilung der Stapesfußplatte bei Revisionsstapesplastik und zur Orientierung bei der Kochleaimplantation, vorwiegend bei Missbildungen, geht. Die OCT bildet Mittel- und Innenohrstrukturen präzise ab und ermöglicht, diese zur Therapiefestlegung zu nutzen. Aus den eigenen bisherigen Ex- und In-vivo-Untersuchungen mit einem OCT-System, das an ein Operationsmikroskop gekoppelt ist, lassen sich derzeit – unter Berücksichtigung der bislang publizierten experimentellen Arbeiten – potenzielle Anwendungsgebiete definieren: Darstellung der ovalen Fensternische bei Revisionsstapes- und rekonstruktiver Mittelohrchirurgie sowie bei der explorativen Tympanotomie zur Abklärung von Perilymphfisteln und Visualisierung von Strukturen des nicht eröffneten Innenohrs.},
       keywords = {Medizin},
       year = {2009}
    }
  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {Using Smart Source Parsing
    Epub 2009 Oct 29},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       year = {2010}
    }
  • Framme, C. and Walter, A. and Prahs, P. and Regler, R. and Theisen-Kunde, D. and Alt, C. and Brinkmann, R.: Structural changes of the retina after conventional laser photocoagulation and selective retina treatment (SRT) in spectral domain OCT. Curr Eye Res, no. 34, pp. 568-79, 2009
    BibTeX
    @article{Framme,
       author = {Framme, C. and Walter, A. and Prahs, P. and Regler, R. and Theisen-Kunde, D. and Alt, C. and Brinkmann, R.},
       title = {Structural changes of the retina after conventional laser photocoagulation and selective retina treatment (SRT) in spectral domain OCT},
       journal = {Curr Eye Res},
       volume = {34},
       number = {7},
       pages = {568-79},
       note = {Framme, Carsten
    Walter, Andreas
    Prahs, Philipp
    Regler, Roman
    Theisen-Kunde, Dirk
    Alt, Clemens
    Brinkmann, Ralf
    Research Support, Non-U.S. Gov't
    England
    Current eye research
    Curr Eye Res. 2009 Jul;34(7):568-79.},
       abstract = {BACKGROUND: Spectral domain optical coherence tomography (SD-OCT) in patients can deliver retinal cross-sectional images with high resolution. This may allow the evaluation of the extent of damage to the retinal pigment epithelium (RPE) and the neurosensory retina after laser treatment. This article aims to investigate the value of SD-OCT in comparing laser lesions produced by conventional laser photocoagulation and selective retina treatment (SRT). MATERIAL AND METHODS: In a retrospective study, conventional retinal laser (CRL) lesions and SRT laser lesions were evaluated with SD-OCT. One hundred seventy-five CRL lesions were investigated in 10 patients with diabetic maculopathy at timepoints between 1 hr and 4 years after treatment. Ninety-one SRT lesions were examined in 9 patients with central serous retinopathy, geographic atrophy, and diabetic maculopathy at timepoints between 1 hr and 2 years. CRL lesions were applied with an ophthalmoscopically slightly grayish-white appearance (Nd:YAG laser at 532-nm wavelength; power 100-200 mW; retinal spot diameter 100 microm; pulse duration 100 ms). SRT lesions were applied with a Nd:YLF (527 nm; pulse duration 200 ns [30 pulses at 100 Hz]; energy 100-200 microJ/pulse; retinal spot diameter 200 microm) and were visible only angiographically. RESULTS: All CRL lesions were characterized by high reflectivity in OCT images throughout the full thickness of the neurosensory tissue 1 hr after irradiation, suggesting complete neurosensory coagulation. Strong contraction through the full thickness of the neurosensory layers was observed within 7 days after treatment. In contrast, the neural retina appeared unaffected after SRT. For both lesion types, the RPE layer appeared to be regular or thinner immediately after treatment, whereas within a period of 4 weeks, a RPE thickening indicating RPE proliferation was observable. One year and later after treatment, CRL lesions were characterized by RPE atrophy combined with significant damage of the neurosensory tissue. SRT lesions aged one year and older revealed unaffected neurosensory structures and an intact RPE layer. CONCLUSION: Spectral domain OCT can be used clinically to follow the development of laser-induced lesions over time. Postoperative RPE proliferation was observed in both CRL and SRT laser lesions. RPE atrophy appeared subsequently only in CRL lesions, whereas the neurosensory retina appeared unaffected following SRT. These results suggest the selective effect of SRT in humans without causing adverse effects to the neurosensory retina.},
       keywords = {Adult
    Aged
    Aged, 80 and over
    Atrophy
    Humans
    Laser Coagulation/*adverse effects
    Lasers, Solid-State/*adverse effects
    Middle Aged
    *Postoperative Complications
    Retina/*pathology
    Retinal Diseases/*diagnosis/*surgery
    Retinal Pigment Epithelium/pathology
    Retrospective Studies
    *Tomography, Optical Coherence},
       year = {2009}
    }
  • Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.: Imaging of human brain tumor tissue by near-infrared laser coherence tomography. Acta Neurochir (Wien), no. 151, pp. 507-17; discussion 517, 2009
    BibTeX
    @article{Böhringer,
       author = {Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.},
       title = {Imaging of human brain tumor tissue by near-infrared laser coherence tomography},
       journal = {Acta Neurochir (Wien)},
       volume = {151},
       number = {5},
       pages = {507-17; discussion 517},
       note = {Bohringer, H J
    Lankenau, E
    Stellmacher, F
    Reusche, E
    Huttmann, G
    Giese, A
    Austria
    Acta Neurochir (Wien). 2009 May;151(5):507-17; discussion 517. Epub 2009 Apr 3.},
       abstract = {INTRODUCTION: Intraoperative detection of residual tumor remains an important challenge in surgery to treat gliomas. New developments in optical techniques offer non-invasive high-resolution imaging that may integrate well into the workflow of neurosurgical operations. Using an intracranial glioma model, we have recently shown that time domain optical coherence tomography (OCT) allows discrimination of normal brain, diffusely invaded brain tissue, and solid tumor. OCT imaging allowed acquisition of 2D and 3D data arrays for multiplanar analysis of the tumor to brain interface. In this study we have analyzed biopsy specimens of human brain tumors and we present the first feasibility study of intraoperative OCT and post-image acquisition processing for non-invasive imaging of the brain and brain tumor. METHODS: We used a Sirius 713 Tomograph with a superluminescence diode emitting light at a near infrared central wavelength of 1,310 nm and a coherence length of 15 microm. The light is passed through an optical mono mode fiber to a modified OCT adapter containing a lens system with a working distance of 10 cm and an integrated pilot laser. Navigation-registered tumor biopsies were imaged ex vivo and the intraoperative site of optical tissue analysis was registered by marker acquisition using a neuronavigation system. RESULTS: Optical coherence tomography non-contact measurements of brain and brain tumor tissue produced B-scan images of 4 mm in width and 1.5-2.0 mm in depth at an axial and lateral optical resolution of 15 microm. OCT imaging demonstrated a different microstructure and characteristic signal attenuation profiles of tumor versus normal brain. Post-image acquisition processing and automated detection of the tissue to air interface was used to realign A-scans to compensate for image distortions caused by pulse- and respiration-induced movements of the target volume. Realigned images allowed monitoring of intensity changes within the scan line and facilitated selection of areas for the averaging of A-scans and the calculation of attenuation coefficients for specific regions of interest. CONCLUSION: This feasibility study has demonstrated that OCT analysis of the tissue microstructure and light attenuation characteristics discriminate normal brain, areas of tumor infiltrated brain, solid tumor, and necrosis. The working distance of the OCT adapter and the A-scan acquisition rate conceptually allows integration of the OCT applicator into the optical path of the operating microscopes. This would allow a continuous analysis of the resection plain, providing optical tomography, thereby adding a third dimension to the microscopic view and information on the light attenuation characteristics of the tissue.},
       keywords = {Biopsy
    Brain Neoplasms/*diagnosis/pathology/surgery
    Feasibility Studies
    Glioma/*diagnosis/pathology/surgery
    Humans
    Monitoring, Intraoperative/methods
    Pilot Projects
    Tomography, Optical Coherence/*methods},
       year = {2009}
    }
  • Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.: Automated segmentation of tissue structures in optical coherence tomography data. J Biomed Opt, no. 14, pp. 034046, 2009
    BibTeX
    @article{Gasca,
       author = {Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.},
       title = {Automated segmentation of tissue structures in optical coherence tomography data},
       journal = {J Biomed Opt},
       volume = {14},
       number = {3},
       pages = {034046},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Segmentation of optical coherence tomography (OCT) images provides useful information, especially in medical imaging applications. Because OCT images are subject to speckle noise, the identification of structures is complicated. Addressing this issue, two methods for the automated segmentation of arbitrary structures in OCT images are proposed. The methods perform a seeded region growing, applying a model-based analysis of OCT A-scans for the seed's acquisition. The segmentation therefore avoids any user-intervention dependency. The first region-growing algorithm uses an adaptive neighborhood homogeneity criterion based on a model of an OCT intensity course in tissue and a model of speckle noise corruption. It can be applied to an unfiltered OCT image. The second performs region growing on a filtered OCT image applying the local median as a measure for homogeneity in the region. Performance is compared through the quantitative evaluation of artificial data, showing the capabilities of both in terms of structures detected and leakage. The proposed methods were tested on real OCT data in different scenarios and showed promising results for their application in OCT imaging.},
       year = {2009}
    }
  • Freidank, S. and Linz, N.: Mit der biomedizinischen Optik hoch hinaus - Lübecker Projekt zur Laserforschung auf der Zugspitze. Focus Uni Luebeck / Universität Lübeck, no. 26, pp. 16, 2009
    BibTeX
    @article{Freidank,
       author = {Freidank, S. and Linz, N.},
       title = {Mit der biomedizinischen Optik hoch hinaus - Lübecker Projekt zur Laserforschung auf der Zugspitze },
       journal = {Focus Uni Luebeck / Universität Lübeck},
       volume = {26},
       number = {1},
       pages = {16},
       year = {2009}
    }
  • Hillmann, Dierck and Huttmann, Gereon and Koch, Peter: Using nonequispaced fast Fourier transformation to process optical coherence tomography signals. no. 7372, pp. 73720R, SPIE,
    BibTeX
    @inproceedings{Hillmann,
       author = {Hillmann, Dierck and Huttmann, Gereon and Koch, Peter},
       title = {Using nonequispaced fast Fourier transformation to process optical coherence tomography signals},
       editor = {Peter, E. Andersen and Brett, E. Bouma},
       publisher = {SPIE},
       volume = {7372},
       pages = {73720R},
    
    }
  • Probst, J. and Koch, P. and Hüttmann, G: Real Time 3D Rendering of Coherence Tomography Volumetric Data. 14.-16.06.2009, 2009
    BibTeX
    @techreport{Probst,
       author = {Probst, J. and Koch, P. and Hüttmann, G},
       title = {Real Time 3D Rendering of Coherence Tomography Volumetric Data},
       month = {14.-16.06.2009},
       year = {2009}
    }
  • Miura, Y. and Roider, J.: Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells. Graefes Arch Clin Exp Ophthalmol, no. 247, pp. 641-9, 2009
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Roider, J.},
       title = {Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {247},
       number = {5},
       pages = {641-9},
       note = {Using Smart Source Parsing
    May; Epub 2009 Feb 3},
       abstract = {PURPOSE: Oxidative stress is known to disrupt the integrity of retinal pigment epithelium (RPE) tight junctions. The goal of this study is to evaluate the effect of triamcinolone acetonide (TA) on the junctional integrity of RPE under oxidative stress and to identify the underlying mechanisms. METHODS: Second passage porcine RPE cells were cultured on 6-well membrane inserts until 4 weeks after reaching confluence. Cells were incubated with TA (10(-5) M) for 30 min. FITC-containing medium was added to the upper chamber (cell's apical side). The cells were then challenged with 1 mM Hydrogen Peroxide (H(2)O(2)). After 5 h, the fluorescence intensity of the medium from lower chamber (cell's basolateral side) was measured using a fluorescence spectrofluorophotometer. This transepithelial flux of FITC-dextran was measured until the 21st day. The immunolocalization of occludin and F-actin was examined with fluorescence microscope. Reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio was determined by a colorimetric assay kit. RESULTS: Non-lethal oxidative stress by H(2)O(2) increased transepithelial flux of FITC-dextran significantly. TA inhibited this increase and preserved the lower flux through the whole experimental period. This permeability change by H(2)O(2) was reversible and recovered to the normal level within 3 weeks. In immunohistological study, H(2)O(2) reduced linear occludin staining at the cell border and increased actin stress fibers. TA prevented H(2)O(2)-induced disruption of junctional assembly of occludin and F-actin. Glutathione assay demonstrated that intracellular GSH/GSSG ratio decreased significantly with H(2)O(2), while TA preserved this ratio by up-regulating GSH synthesis. CONCLUSIONS: TA has a protective effect against oxidative stress-induced disruption of RPE tight junction by preserving cellular redox state.},
       year = {2009}
    }
  • Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.: Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density. Acta Neurochir, no. 151, pp. 253-262, 2009
    BibTeX
    @article{Kandelhardt,
       author = {Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.},
       title = {Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density},
       journal = {Acta Neurochir },
       volume = {151},
       pages = {253-262},
       year = {2009}
    }
  • Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.: Automated segmentation of tissue structures in optical coherence tomography data. J Biomed Opt, no. 14, pp. 034046, 2009
    BibTeX
    @article{Gasca,
       author = {Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.},
       title = {Automated segmentation of tissue structures in optical coherence tomography data},
       journal = {J Biomed Opt},
       volume = {14},
       number = {3},
       pages = {034046},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Segmentation of optical coherence tomography (OCT) images provides useful information, especially in medical imaging applications. Because OCT images are subject to speckle noise, the identification of structures is complicated. Addressing this issue, two methods for the automated segmentation of arbitrary structures in OCT images are proposed. The methods perform a seeded region growing, applying a model-based analysis of OCT A-scans for the seed's acquisition. The segmentation therefore avoids any user-intervention dependency. The first region-growing algorithm uses an adaptive neighborhood homogeneity criterion based on a model of an OCT intensity course in tissue and a model of speckle noise corruption. It can be applied to an unfiltered OCT image. The second performs region growing on a filtered OCT image applying the local median as a measure for homogeneity in the region. Performance is compared through the quantitative evaluation of artificial data, showing the capabilities of both in terms of structures detected and leakage. The proposed methods were tested on real OCT data in different scenarios and showed promising results for their application in OCT imaging.},
       year = {2009}
    }
  • Fritz, A and Ptaszynski, L and Stoehr, H and Brinkmann, R: Dynamic of laser induced transient microbubble clusters. Proc SPIE,
    BibTeX
    @inproceedings{Fritz2009,
       author = {Fritz, A and Ptaszynski, L and Stoehr, H and Brinkmann, R},
       title = {Dynamic of laser induced transient microbubble clusters},
       editor = {(BiOS), Conference on Biomedical Optics},
       publisher = {Proc SPIE},
       type = {Conference Proceedings}
    }
    
  • Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P: A 75 MHz light source for femtosecond stimulated raman microscopy. Optics express, no. 17, pp. 18612-20, oct,, 2009
    BibTeX Link
    @Article{HU_2009_Ploetz_a,
      Title                    = {{A 75 MHz light source for femtosecond stimulated raman microscopy}},
      Author                   = {Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {18612--20},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.018612},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik and Center of Integrated Protein Science Munich Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen Oettingenstr 67, D-80538 M\"{u}nchen, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-21-18612}
    }
    
  • Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert: A theoretical description of Fourier domain mode locked lasers. Optics express, no. 17, pp. 24013-9, dec,, 2009
    BibTeX Link
    @Article{HU_2009_Jirauschek_a,
      Title                    = {{A theoretical description of Fourier domain mode locked lasers}},
      Author                   = {Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = dec,
      Number                   = {26},
      Pages                    = {24013--9},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.024013},
      Institution              = {Institute for Nanoelectronics, Technische Universit\"{a}t M\"{u}nchen, Arcisstrasse 21, D-80333 Munich, Germany. jirauschek@tum.de},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-26-24013}
    }
  • Huber, Robert: Advances in Fourier domain OCT. in 2009 IEEE LEOS Annual Meeting Conference Proceedings, pp. 201-202, IEEE, oct,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Huber_a,
      Title                    = {{Advances in Fourier domain OCT}},
      Author                   = {Huber, Robert},
      Booktitle                = {2009 IEEE LEOS Annual Meeting Conference Proceedings},
      Year                     = {2009},
      Month                    = oct,
      Pages                    = {201--202},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/LEOS.2009.5343314},
      ISBN                     = {978-1-4244-3680-4},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5343314}
    }
    
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Dispersion, coherence and noise of Fourier Domain Mode Locked (FDML) lasers. in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Biedermann_b,
      Title                    = {{Dispersion, coherence and noise of Fourier Domain Mode Locked (FDML) lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5192900},
      ISBN                     = {978-1-4244-4079-5},
      Pmid                     = {19506645},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5192900}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Dispersion, coherence and noise of Fourier domain mode locked lasers. Optics Express, no. 17, pp. 9947 - 9961, may,, 2009
    BibTeX Link
    @Article{HU_2009_Biedermann_a,
      Title                    = {{Dispersion, coherence and noise of Fourier domain mode locked lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Journal                  = {Optics Express},
      Year                     = {2009},
    
      Month                    = may,
      Number                   = {12},
      Pages                    = {9947 -- 9961 },
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.009947},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen, Munich, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-12-9947}
    }
  • Palte, Gesa and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Huber, Robert: Fourier domain mode locked (FDML) lasers for polarization sensitive OCT. in European Conferences on Biomedical Optics, pp. 73720M-73720M-6, International Society for Optics and Photonics, jul,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Palte_a,
      Title                    = {{Fourier domain mode locked (FDML) lasers for polarization sensitive OCT}},
      Author                   = {Palte, Gesa and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Huber, Robert},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Andersen, Peter E and Bouma, Brett E},
      Month                    = jul,
      Pages                    = {73720M--73720M--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.831835},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1339476}
    }
  • Huber, Robert: Fourier domain mode locking: new lasers for optical coherence tomography. in SPIE Newsroom, 2009
    BibTeX Link
    @Misc{HU_2009_Huber_b,
      Title                    = {{Fourier domain mode locking: new lasers for optical coherence tomography}},
    
      Author                   = {Huber, Robert},
      Year                     = {2009},
    
      Booktitle                = {SPIE Newsroom},
      Doi                      = {10.1117/2.1200901.1440},
      ISSN                     = {18182259},
      Url                      = {http://www.spie.org/x33321.xml}
    }
  • Leonhardt, Rainer and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Nonlinear optical frequency conversion of an amplified Fourier Domain Mode Locked (FDML) laser.. Optics express, no. 17, pp. 16801-8, OSA, sep,, 2009
    BibTeX Link
    @Article{HU_2009_Leonhardt_a,
      Title                    = {{Nonlinear optical frequency conversion of an amplified Fourier Domain Mode Locked (FDML) laser.}},
      Author                   = {Leonhardt, Rainer and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = sep,
      Number                   = {19},
      Pages                    = {16801--8},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.016801},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=oe-17-19-16801}
    }
  • Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert: Raman pumped Fourier Domain Mode Locked (FDML) laser: Analysis of operation and application for optical coherence tomography (OCT). in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Klein_a,
      Title                    = {{Raman pumped Fourier Domain Mode Locked (FDML) laser: Analysis of operation and application for optical coherence tomography (OCT)}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5194704},
      ISBN                     = {978-1-4244-4079-5},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5194704}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Huber, Robert: Recent developments in Fourier domain mode locked lasers for optical coherence tomography: imaging at 1310 nm vs. 1550 nm wavelength. Journal of biophotonics, no. 2, pp. 357-63, jul,, 2009
    BibTeX Link
    @Article{HU_2009_Biedermann_c,
      Title                    = {{Recent developments in Fourier domain mode locked lasers for optical coherence tomography: imaging at 1310 nm vs. 1550 nm wavelength}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Journal of biophotonics},
      Year                     = {2009},
    
      Month                    = jul,
      Number                   = {6-7},
      Pages                    = {357--63},
      Volume                   = {2},
    
      Doi                      = {10.1002/jbio.200910028},
      ISSN                     = {1864-0648},
      Url                      = {http://onlinelibrary.wiley.com/doi/10.1002/jbio.200910028/abstract;jsessionid=4F65E7D7366C8202661DB8227E57EBEC.f03t04}
    }
    
  • Huber, Robert: State-of-the-art and future of ultrahigh speed OCT. in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Huber_c,
      Title                    = {{State-of-the-art and future of ultrahigh speed OCT}},
      Author                   = {Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5191695},
      ISBN                     = {978-1-4244-4079-5},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5191695}
    }
  • Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert: Subharmonic Fourier domain mode locking. Optics Letters, no. 34, pp. 725 - 727, mar,, 2009
    BibTeX Link
    @Article{HU_2009_Eigenwillig_b,
      Title                    = {{Subharmonic Fourier domain mode locking}},
      Author                   = {Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2009},
    
      Month                    = mar,
      Number                   = {6},
      Pages                    = {725 -- 727 },
      Volume                   = {34},
    
      Doi                      = {10.1364/OL.34.000725},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-34-6-725}
    }
  • Karnowski, Karol and Gora, Michalina and Kaluzny, Bartlomiej and Huber, Robert and Szkulmowski, Maciej and Kowalczyk, Andrzej and Wojtkowski, Maciej: Swept source OCT imaging of human anterior segment at 200 kHz. in SPIE BiOS: Biomedical Optics, pp. 716308-716308-7, International Society for Optics and Photonics, feb,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Karnowski_a,
      Title                    = {{Swept source OCT imaging of human anterior segment at 200 kHz}},
      Author                   = {Karnowski, Karol and Gora, Michalina and Kaluzny, Bartlomiej and Huber, Robert and Szkulmowski, Maciej and Kowalczyk, Andrzej and Wojtkowski, Maciej},
      Booktitle                = {SPIE BiOS: Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Manns, Fabrice and S\"{o}derberg, Per G and Ho, Arthur},
      Month                    = feb,
      Pages                    = {716308--716308--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.808555},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1331711}
    }
  • Gora, Michalina and Karnowski, Karol and Szkulmowski, Maciej and Kaluzny, Bartlomiej J and Huber, Robert and Kowalczyk, Andrzej and Wojtkowski, Maciej: Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range. Optics Express, no. 17, pp. 14880-14894, aug,, 2009
    BibTeX Link
    @Article{HU_2009_Gora_a,
      Title                    = {{Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range}},
      Author                   = {Gora, Michalina and Karnowski, Karol and Szkulmowski, Maciej and Kaluzny, Bartlomiej J and Huber, Robert and Kowalczyk, Andrzej and Wojtkowski, Maciej},
      Journal                  = {Optics Express},
      Year                     = {2009},
    
      Month                    = aug,
      Number                   = {17},
      Pages                    = {14880--14894 },
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.014880},
      Institution              = {Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5/7, PL-87-100 Toruń, Poland.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-17-14880}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: Ultra-rapid dispersion measurement in optical fibers. Optics express, no. 17, pp. 22871-8, dec,, 2009
    BibTeX Link
    @Article{HU_2009_Wieser_a,
      Title                    = {{Ultra-rapid dispersion measurement in optical fibers}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = dec,
      Number                   = {25},
      Pages                    = {22871--8},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.022871},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-25-22871}
    }
  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Wavelength swept amplified spontaneous emission source. Optics express, no. 17, pp. 18794-807, oct,, 2009
    BibTeX Link
    @Article{HU_2009_Eigenwillig_a,
      Title                    = {{Wavelength swept amplified spontaneous emission source}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {18794--807},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.018794},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen Oettingenstr 67, 80538 Munich, Germany.},
      ISSN                     = {1094-4087}
    }
  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Wavelength swept ASE source. in European Conferences on Biomedical Optics, pp. 73720O-73720O-6, International Society for Optics and Photonics, jul,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Eigenwillig_c,
      Title                    = {{Wavelength swept ASE source}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Andersen, Peter E and Bouma, Brett E},
      Month                    = jul,
      Pages                    = {73720O--73720O--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.831831},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=781146}
    }

2008

  • Framme, C. and Roider, J. and Brinkmann, R. and Birngruber, R. and Gabel, V. P.: Basic principles and clinical application of retinal laser therapy. Klinische Monatsblatter Fur Augenheilkunde, no. 225, pp. 259-268, 2008
    BibTeX Link
    @article{Framme2008,
       author = {Framme, C. and Roider, J. and Brinkmann, R. and Birngruber, R. and Gabel, V. P.},
       title = {Basic principles and clinical application of retinal laser therapy},
       journal = {Klinische Monatsblatter Fur Augenheilkunde},
       volume = {225},
       number = {4},
       pages = {259-268},
       note = {301AZ
    Times Cited:3
    Cited References Count:39},
       abstract = {The scientific background of laser photocoagulation of the ocular fundus was studied extensively by several investigators in the 1970 s and 1980 s. The basic principles were succesfully resolved during that time and clinical consequences for proper application of the laser photocoagulation for various diseases were deduced. The present paper gives an overview about the physical basics of laser-tissue interactions during and after retinal laser treatment and the particular laser strategies in the treatment of different retinal diseases. Thus, it addresses the issue of the impact on tissue of laser parameters as wavelength, spot size, pulse duration and laser power. Additionally, the different biological tissue reactions after laser treatment are presented, such as, e.g., for retinopexia or macular treatments as well as for diabetic retinopathies. Specific laser strategies such as the selective laser treatment of the RPE (SRT) or the transpupillary thermotherapy (TTT) are presented and discussed.},
       keywords = {retina
    anatomy
    vitreous
    subfoveal choroidal neovascularization
    central vein occlusion
    transpupillary thermotherapy
    macular degeneration
    pigment epithelium
    photocoagulation
    argon
    trial
    rpe
    diseases},
       ISSN = {0023-2165},
       DOI = {DOI 10.1055/s-2008-1027202},
       url = {<Go to ISI>://WOS:000255870100001},
       year = {2008},
       type = {Journal Article}
    }
    
  • Framme, C. and Sachs, H. G. and Kobuch, K. and Flucke, B. and Birngruber, R.: Clinical evaluation of experimentally induced choroidal neovascularizations in Pigmented rabbits by subretinal injection of lipid hydroperoxide and consecutive preliminary photodynamic treatment with tookad. Ophthalmologica, no. 222, pp. 254-264, 2008
    BibTeX Link
    @article{Framme2008,
       author = {Framme, C. and Sachs, H. G. and Kobuch, K. and Flucke, B. and Birngruber, R.},
       title = {Clinical evaluation of experimentally induced choroidal neovascularizations in Pigmented rabbits by subretinal injection of lipid hydroperoxide and consecutive preliminary photodynamic treatment with tookad},
       journal = {Ophthalmologica},
       volume = {222},
       number = {4},
       pages = {254-264},
       note = {338UZ
    Times Cited:3
    Cited References Count:27},
       abstract = {Purpose: Up to date several approaches have been undertaken to achieve an 'easy-to-handle' animal model of choroidal neovascularizations (CNVs) in rabbits; however, so far in none of the studies could healthy retinal tissue be maintained, which is mandatory to further investigate the effects of photodynamic therapy (PDT) or anti-vascular-endothelial-growth-factor treatments. It was our aim to reevaluate and verify the method of inducing experimental CNVs in rabbits using subretinally injected linoleic acid hydroperoxicle (LHP) as proposed by Tamai et al. and to use it for experimental PDT. Material and Methods: In 33 eyes of Chinchilla breed rabbits LHP of two different concentrations (25 and 100 mu g/50 mu l) was injected into the subretinal space via a transvitreal approach under guidance of an operation microscope. Ophthalmoscopic and angiographic examinations were performed on days 3, 7, 14 and 28 after surgery. Preliminary PDT with different experimental parameter sets was performed in 3 eyes using the new photosensitizer Tookad. Results: Using LHP in the higher concentration, an angiographically determined CNV induction was observed in 27% of all injection sites (n = 34) on days 14 and 28 revealing early well-demarcated and progressive leakage. No CNV was detected at the lower LHP concentration (60 injection sites). Subretinal CNV was verified histologically revealing vessel formation above the retinal pigment epithelium level. Herein, a significant damage to the outer retinal layers was always observed; however, the general structure of the choriocapillary layer was maintained. Tookad PDT was clinically able to completely stop leakage in I case and reduce leakage in 2 cases. Histologically the choriocapillary layer was occluded. Conclusion: Subretinal injection of LHP induces angiographically well-demarcated classic CNVs in rabbits; however, the CNV rate was low, and histology revealed severe damage of the outer retinal layers but not of the choriocapillary layer, which is important for studying PDT interactions. Preliminary experimental PDT could clinically stop or reduce leakage from angiographic CNV. Due to the small CNV rate and the significant collateral retinal tissue damage, this model seems to be only of partial suitability for investigating new treatment modalities in CNV. Copyright (c) 2008 S. Karger AG, Basel.},
       keywords = {age-related macular degeneration
    animal model
    choroidal neovascularization
    retinal pigment epithelium
    linoleic acid hydroperoxide
    senile macular degeneration
    bruchs membrane
    ranibizumab
    verteporfin
    therapy
    growth
    model},
       ISSN = {0030-3755},
       DOI = {Doi 10.1159/000136903},
       url = {<Go to ISI>://WOS:000258536200008},
       year = {2008},
       type = {Journal Article}
    }
    
  • Neumann, J and Brinkmann, R: Self-limited growth of laser-induced vapor bubbles around single micro-absorbers. Appl Phys Lett, no. 93, pp. 033901, 2008
    BibTeX
    @article{Neumann,
       author = {Neumann, J and Brinkmann, R},
       title = {Self-limited growth of laser-induced vapor bubbles around single micro-absorbers},
       journal = {Appl  Phys Lett},
       volume = {93},
       pages = {033901},
       year = {2008}
    }
  • Mueller, M. and Huettmann, G. and Koop, N. and Steven, P.: Minimal-Invasive Imaging of Ocular Surface Pathologies - Confocal vs. Two-Photon Microscopy. Investigative Ophthalmology & Visual Science, no. 49, pp. 2258-2258, 2008
    BibTeX Link
    @article{Mueller2008,
       author = {Mueller, M. and Huettmann, G. and Koop, N. and Steven, P.},
       title = {Minimal-Invasive Imaging of Ocular Surface Pathologies - Confocal vs. Two-Photon Microscopy},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {49},
       number = {13},
       pages = {2258-2258},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2008},
       type = {Journal Article}
    }
    
  • Sandeau, J. and Caillibotte, G. and Kandulla, J. and Elsner, H. and Brinkmann, R and Birngruber, R. and Apiou-Sbirlea, G.: Numerical Modelling of Conductive and Convective Heat Transfers in Retinal Laser Applications. no. 1, pp. 43-52, 2008
    BibTeX
    @misc{Sandeau,
       author = {Sandeau, J. and Caillibotte, G. and Kandulla, J. and Elsner, H. and Brinkmann, R and Birngruber, R. and Apiou-Sbirlea, G.},
       title = {Numerical Modelling of Conductive and Convective Heat Transfers in Retinal  Laser Applications},
       volume = {1},
       number = {1},
       pages = {43-52},
       year = {2008}
    }
  • Framme, C and Roider, J and Brinkmann, R and Birngruber, R and Gabel, V-P: Grundlagen und klinische Anwendung der Lasertherapie an der Netzhaut. no. 225, pp. 259-268, 2008
    BibTeX
    @misc{Framme,
       author = {Framme, C and Roider, J and Brinkmann, R and Birngruber, R and Gabel, V-P},
       title = {Grundlagen und klinische Anwendung der Lasertherapie an der Netzhaut},
       volume = {225},
       number = {4},
       pages = {259-268},
       year = {2008}
    }
  • Framme, C. and Walter, A. and Prahs, P. and Theisen-Kunde, D. and Brinkmann, R.: Comparison of threshold irradiances and online dosimetry for selective retina treatment (SRT) in patients treated with 200 nanoseconds and 1.7 microseconds laser pulses. Lasers Surg Med, no. 40, pp. 616-24, 2008
    BibTeX
    @article{Framme,
       author = {Framme, C. and Walter, A. and Prahs, P. and Theisen-Kunde, D. and Brinkmann, R.},
       title = {Comparison of threshold irradiances and online dosimetry for selective retina treatment (SRT) in patients treated with 200 nanoseconds and 1.7 microseconds laser pulses},
       journal = {Lasers Surg Med},
       volume = {40},
       number = {9},
       pages = {616-24},
       note = {Framme, Carsten
    Walter, Andreas
    Prahs, Philipp
    Theisen-Kunde, Dirk
    Brinkmann, Ralf
    Clinical Trial
    Comparative Study
    Research Support, Non-U.S. Gov't
    United States
    Lasers Surg Med. 2008 Nov;40(9):616-24.},
       abstract = {BACKGROUND: Selective retina therapy (SRT) solely affecting the RPE while sparing of the photoreceptors is usually performed with a train of repetitive laser pulses of 1.7 microseconds in duration. It was our purpose to evaluate the principle feasibility of SRT with shorter 200 nanoseconds laser pulses in patients. METHODS: Nineteen patients with macular disorders [diabetic maculopathy (DMP), geographic atrophy (GA), drusen maculopathy and central serous chorioretinopathy (CSR)] were treated with a prototype of a SRT laser (Nd:YLF laser; 527 nm; 1.7 microseconds and 200 nanoseconds pulse duration; 30 pulses at 100 Hz; spot size: 200 microm). Test lesions (n = 175) with increasing energy were applied at the lower arcade to determine the individual angiographic and ophthalmoscopic threshold radiant exposures (therapeutic window) before applying the central treatment lesions within these ranges additionally guided by online optoacoustic measurements. Postoperatively RPE damage was visualized and confirmed by fluorescein angiographic leakage and correlated with optoacoustic results. Additionally ED(50) damage thresholds were calculated by probit analysis. RESULTS: None of the short repetitive 200 nanoseconds laser pulses led to retinal hemorrhages or retinal ruptures. Nearly all of the test- and treatment lesions could be visualized by angiography indicating desired RPE damage but were ophthalmoscopically invisible suggesting intact neurosensory retinal structures. ED(50) cell damage threshold energies were significantly lower using 200 nanoseconds (99.6 microJ; n = 122) instead of 1.7 microseconds (196.3 microJ; n = 53) laser pulses. Optoacoustic and angiographic visibility correlated in 83.7% (200 nanoseconds) and 87.5% (1.7 microseconds). CONCLUSIONS: Selective RPE effects can safely be achieved using shorter 200 nanoseconds laser pulses in patients without adverse effects to the neurosensory retina. The required pulse energy compared to the standard 1.7 microseconds regime was reduced by about a factor of 2 suggesting a reduced heat generation and flow into adjacent tissues during the shorter laser impact and thus possibly enhancing selectivity. Optoacoustics also seem to be a viable alternative in 200 nanoseconds treatment for a non-invasive online dosimetry control system.},
       keywords = {Adult
    Aged
    Aged, 80 and over
    Cohort Studies
    Feasibility Studies
    Female
    Humans
    Laser Coagulation/ methods
    Lasers, Semiconductor/ therapeutic use
    Male
    Middle Aged
    Ophthalmoscopy
    Radiometry
    Retinal Diseases/pathology/radiography/ surgery
    Retinal Pigment Epithelium/pathology/radiation effects/radiography},
       year = {2008}
    }
    
  • Vogel, A. and Linz, N. and Freidank, S. and Paltauf, G.: Femtosecond-laser-induced nanocavitation in water: implications for optical breakdown threshold and cell surgery. no. 100, pp. 23, 2008
    BibTeX
    @misc{Vogel2008,
       author = {Vogel, A. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Femtosecond-laser-induced nanocavitation in water: implications for optical breakdown threshold and cell surgery},
       volume = {100},
       number = {3},
       pages = {23},
       note = {Using Smart Source Parsing
    Jan 25;:038102. Epub 2008 Jan},
       abstract = {We determined the bubble radius R_(max) for femtosecond optical breakdown in water at 347, 520, and 1040 nm with an unprecedented accuracy (+/-10 nm). At threshold, R_(max) was smaller than the diffraction-limited focus radius and ranged from 190 nm to 320 nm. The increase of R_(max) with laser energy E_(L) is slowest at 347 nm, providing optimum control of cell surgery. Experimental results agree with a model of bubble formation in heated and thermoelastically stretched liquids. Theory predicts a threshold temperature T_(th) approximately equal to 168 degrees C. For T>300 degrees C, a phase explosion sets in, and R_(max) increases rapidly with E_(L).},
       ISBN = {0031-9007 (Print)
    0031-9007 (Linking)},
       year = {2008}
    }
  • Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.: Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests. in Plant J, no. 54, pp. 938-48, 2008
    BibTeX
    @incollection{Phillips,
       author = {Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.},
       title = {Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests},
       booktitle = {Plant J},
       volume = {54},
       edition = {2008/03/19},
       pages = {938-48},
       note = {Phillips, Jonathan R
    Fischer, Eberhard
    Baron, Miriam
    van den Dries, Niels
    Facchinelli, Fabio
    Kutzer, Michael
    Rahmanzadeh, Ramtin
    Remus, Daniela
    Bartels, Dorothea
    England
    Plant J. 2008 Jun;54(5):938-48. Epub 2008 Mar 13.},
       abstract = {A particular adaptation to survival under limited water availability has been realized in the desiccation-tolerant resurrection plants, which tend to grow in a habitat with seasonal rainfall and long dry periods. One of the best-studied examples is Craterostigma plantagineum. Here we report an unexpected finding: Lindernia brevidens, a close relative of C. plantagineum, exhibits desiccation tolerance, even though it is endemic to the montane rainforests of Tanzania and Kenya, where it never experiences seasonal dry periods. L. brevidens has been found exclusively in two fragments of the ancient Eastern Arc Mountains, which were protected from the devastating Pleistocene droughts by the stable Indian Ocean temperature. Analysis of the microhabitat reveals that L. brevidens is found in the same habitat as hygrophilous plant species, which further indicates that the plant never dries out completely. The objective of this investigation was to address whether C. plantagineum and L. brevidens have desiccation-related pathways in common, or whether L. brevidens has acquired novel pathways. A third, closely related, desiccation-sensitive species, Lindernia subracemosa, has been included for comparison. Mechanisms that confer cellular protection during extreme water loss are well conserved between C. plantagineum and L. brevidens, including the interconversion of 2-octulose to sucrose within the two desiccation-tolerant species. Furthermore, transcriptional control regions of desiccation-related genes belonging to the late embryogenesis abundant (LEA) protein family are also highly conserved. We propose that L. brevidens is a neoendemic species that has retained desiccation tolerance through genome stability, despite tolerance being superfluous to environmental conditions.},
       keywords = {Adaptation, Physiological
    Gene Expression Profiling
    Genome, Plant
    Lamiaceae/genetics/metabolism/ physiology
    Sucrose/metabolism
    Tropical Climate
    Water},
       year = {2008}
    }
  • Framme, C and Schüle, G and Kobuch, K and Flucke, B and Birngruber, R and Brinkmann, R: Investigation of Selective Retina Treatment (SRT) by Means of 8 ns Laser Pulses in a Rabbit Model. no. 40, pp. 20-27, 2008
    BibTeX
    @misc{Framme,
       author = {Framme, C and Schüle, G and Kobuch, K and Flucke, B and Birngruber, R and Brinkmann, R},
       title = {Investigation of Selective Retina Treatment (SRT) by Means of 8 ns Laser Pulses in a Rabbit Model},
       volume = {40},
       pages = {20-27},
       year = {2008}
    }
  • Just, T. and Lankenau, E. and Huettmann, G. and Pau, H.W.: Optical coherence tomography as a guide for cochlear implant surgery. in Progress in Biomedical Optics and Imaging, pp. F1-F6, SPIE 6842,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Lankenau, E. and Huettmann, G. and Pau, H.W.},
       title = {Optical coherence tomography as a guide for cochlear implant surgery},
       booktitle = {Progress in Biomedical Optics and Imaging},
       editor = {Nikiforos, K. and Bernard, C. and Haishan, Z.},
       publisher = {SPIE 6842},
       pages = {F1-F6},
    
    }
  • Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W: Optically controlled thermal management on the nanometer length scale. Nanotechnology, no. 19, pp. 055207, 2008
    BibTeX
    @article{Garwe,
       author = {Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W},
       title = {Optically controlled thermal management on the nanometer length scale},
       journal = {Nanotechnology},
       volume = {19},
       number = {5},
       pages = {055207},
       abstract = {The manipulation of polymers and biological molecules or the control of chemical reactions on a nanometer scale by means of laser pulses shows great promise for applications in modern nanotechnology, biotechnology, molecular medicine or chemistry. A controllable, parallel, highly efficient and very local heat conversion of the incident laser light into metal nanoparticles without ablation or fragmentation provides the means for a tool like a 'nanoreactor', a 'nanowelder', a 'nanocrystallizer' or a 'nanodesorber'. In this paper we explain theoretically and show experimentally the interaction of laser radiation with gold nanoparticles on a polymethylmethacrylate (PMMA) layer (one-photon excitation) by means of different laser pulse lengths, wavelengths and pulse repetition rates. To the best of our knowledge this is the first report showing the possibility of highly local (in a 40 nm range) regulated heat insertion into the nanoparticle and its surroundings without ablation of the gold nanoparticles. In an earlier paper we showed that near-infrared femtosecond irradiation can cut labeled DNA sequences in metaphase chromosomes below the diffraction-limited spot size. Now, we use gold as well as silver-enhanced gold nanoparticles on DNA (also within chromosomes) as energy coupling objects for femtosecond laser irradiation with single-and two-photon excitation. We show the results of highly localized destruction effects on DNA that occur only nearby the nanoparticles.},
       year = {2008}
    }
  • Koinzer, S and Elsner, H and Klatt, C and Brinkmann, R and Birngruber, R and Roider, J: Selective Retina Therapy (SRT) of chronic subfoveal fluid after surgery of rhegmatogenous retinal detachment: three case reports. no. 246, pp. 1373-8, 2008
    BibTeX
    @misc{Koinzer,
       author = {Koinzer, S and Elsner, H and Klatt, C and Brinkmann, R and Birngruber, R and Roider, J},
       title = {Selective Retina Therapy (SRT) of chronic subfoveal fluid after surgery of rhegmatogenous retinal detachment: three case reports},
       volume = {246},
       number = {10},
       pages = {1373-8},
       year = {2008}
    }
  • Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.: Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental. Laser Surg Med, no. 40, pp. 273-81, 2008
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.},
       title = {Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental},
       journal = {Laser Surg Med},
       volume = {40
    },
       number = {4},
       pages = {273-81},
       year = {2008}
    }
  • Ramrath, L. and Moreno, G. and Mueller, H. and Bonin, T. and Huettmann, G. and Schweikard, A.: Towards multi-directional OCT for speckle noise reduction. Med Image Comput Comput Assist Interv, no. 11, pp. 815-23, 2008
    BibTeX
    @article{Ramrath,
       author = {Ramrath, L. and Moreno, G. and Mueller, H. and Bonin, T. and Huettmann, G. and Schweikard, A.},
       title = {Towards multi-directional OCT for speckle noise reduction},
       journal = {Med Image Comput Comput Assist Interv},
       volume = {11},
       number = {Pt 1},
       pages = {815-23},
       note = {Using Smart Source Parsing},
       abstract = {Multi-directional optical coherence tomography (MD-OCT) applies and extends the concept of angular compounding for speckle noise reduction to the area of OCT imaging. OCT images are acquired from a wide range of angles of view. Averaging of the rotated images therefore requires compensation of the parallax which is achieved by simple image registration for image reconstruction. Test measurements of a sample structure in a low and highly scattering environment show that the method improves the signal-to-noise ratio by a factor of 4 and hence reduces speckle noise significantly. Experimental results also show that the proposed averaging increases the performance of common edge-detection algorithms.},
       year = {2008}
    }
  • Steven, Philip and Koop, Norbert and Huttmann, Gereon: Confocal microscopy versus two-photon microscopy: imaging of ocular surface pathologies. no. 6860, pp. 686023, SPIE,
    BibTeX
    @inproceedings{Steven,
       author = {Steven, Philip and Koop, Norbert and Huttmann, Gereon},
       title = {Confocal microscopy versus two-photon microscopy: imaging of ocular surface pathologies},
       editor = {Ammasi, Periasamy and Peter, T. C. So},
       publisher = {SPIE},
       volume = {6860},
       pages = {686023},
    
    }
  • Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.: Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue. Invest Ophthalmol Vis Sci, no. 49, pp. 1512-7, 2008
    BibTeX
    @article{Steven,
       author = {Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.},
       title = {Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {49},
       number = {4},
       pages = {1512-7},
       note = {Using Smart Source Parsing
    Apr},
       abstract = {PURPOSE: Conjunctiva-associated lymphoid tissue (CALT) is assumed to be a key location for the generation of adaptive immune mechanisms of the ocular surface, but functional studies of CALT are still lacking. The purpose of this study was to establish an animal model that enables functional analysis of immune mechanisms going on within CALT. In addition, the use of two-photon microscopy, a new optical method, was evaluated for examining complex immunologic interactions of CALT by volume (three-dimensional [3-D]) and time-dependence (four-dimensional [4-D]) in vivo. METHODS: The conjunctiva of female BALB/c mice was repeatedly challenged with topical Chlamydia trachomatis serovar C or a solution of ovalbumin and cholera toxin B. Two-photon microscopy was conducted on explanted, unfixed, and unstained eyes with adjacent nictitating membranes. RESULTS: After three to five stimulations, CALT was detected exclusively in the nictitating membrane of 73% (C. trachomatis) or 70% (ovalbumin/ cholera toxin) of the animals. CALT mainly consisted of CD45R/B220+ B cells and CD4+ and CD8+ T cells. Electron microscopy showed intraepithelial lymphocytes and follicles consisting of lymphocytes, dendritic cells, and macrophages. Two-photon microscopy based on tissue autofluorescence allowed all components of CALT to be detected three dimensionally. High-resolution images were generated in tissue depths of 65 microm below the mucosal surface. CONCLUSIONS: This study introduces a novel mouse model for functional investigations of CALT. Topical stimulation with C. trachomatis or ovalbumin/cholera toxin B reliably leads to CALT generation at the nictitating membrane. The use of two-photon microscopy enables groundbreaking 3-D and, in the future, intravital 4-D investigations of immunologic processes initiated in CALT.},
       year = {2008}
    }
  • Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.: Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes. no. 13, pp. 031217, 2008
    BibTeX
    @misc{Qu,
       author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.},
       title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes},
       volume = {13},
       number = {3},
       pages = {031217},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.},
       ISBN = {1083-3668 (Print)
    1083-3668 (Linking)},
       year = {2008}
    }
  • Kandulla, J and Brinkmann, R: Non invasive real-time temperature determination during laser treatments at the retina. Photonik international, no. 2008/1, pp. 42-45, 2008
    BibTeX
    @article{Kandulla,
       author = {Kandulla, J and Brinkmann, R},
       title = {Non invasive real-time temperature determination during laser treatments at the retina},
       journal = {Photonik international},
       volume = {2008/1},
       pages = {42-45},
       year = {2008}
    }
  • Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.: Laser-based gene transfection and gene therapy. IEEE Trans Nanobioscience, no. 7, pp. 111-9, 2008
    BibTeX
    @article{Yao,
       author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.},
       title = {Laser-based gene transfection and gene therapy},
       journal = {IEEE Trans Nanobioscience},
       volume = {7},
       number = {2},
       pages = {111-9},
       note = {Yao, C P
    Zhang, Z X
    Rahmanzadeh, R
    Huettmann, G
    Research Support, Non-U.S. Gov't
    Review
    United States
    IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.},
       abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.},
       keywords = {Cell Membrane/ radiation effects
    DNA/ administration & dosage/ pharmacokinetics
    Gene Therapy/ methods
    Lasers
    Transfection/ methods},
       year = {2008}
    }
  • Jirauschek, Christian and Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert: Fourier domain mode locking theory. in 2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS, 2008
    BibTeX Link
    @InProceedings{HU_2008_Jirauschek_a,
      Title                    = {{Fourier domain mode locking theory}},
      Author                   = {Jirauschek, Christian and Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert},
      Booktitle                = {2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS},
      Year                     = {2008},
    
      Url                      = {http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=4571888&searchWithin%3Deigenwillig%26sortType%3Dasc_p_Sequence%26filter%3DAND%28p_IS_Number%3A4571172%29}
    }
  • Andrews, Peter M and Chen, Yu and Onozato, Maristela L and Huang, Shu-Wei and Adler, Desmond C and Huber, Robert A and Jiang, James and Barry, Scott E and Cable, Alex E and Fujimoto, James G: High-resolution optical coherence tomography imaging of the living kidney. Laboratory investigation; a journal of technical methods and pathology, no. 88, pp. 441-9, apr,, 2008
    BibTeX Link
    @Article{HU_2008_Andrews_a,
      Title                    = {{High-resolution optical coherence tomography imaging of the living kidney}},
      Author                   = {Andrews, Peter M and Chen, Yu and Onozato, Maristela L and Huang, Shu-Wei and Adler, Desmond C and Huber, Robert A and Jiang, James and Barry, Scott E and Cable, Alex E and Fujimoto, James G},
      Journal                  = {Laboratory investigation; a journal of technical methods and pathology},
      Year                     = {2008},
    
      Month                    = apr,
      Number                   = {4},
      Pages                    = {441--9},
      Volume                   = {88},
    
      Doi                      = {10.1038/labinvest.2008.4},
      ISSN                     = {1530-0307},
      Url                      = {http://www.nature.com/labinvest/journal/v88/n4/full/labinvest20084a.html}
    }
  • Bajraszewski, Tomasz and Wojtkowski, Maciej and Szkulmowski, Maciej and Szkulmowska, Anna and Huber, Robert and Kowalczyk, Andrzej: Improved spectral optical coherence tomography using optical frequency comb. Optics Express, no. 16, pp. 4163 - 4176, mar,, 2008
    BibTeX Link
    @Article{HU_2008_Bajraszewski_a,
      Title                    = {{Improved spectral optical coherence tomography using optical frequency comb}},
      Author                   = {Bajraszewski, Tomasz and Wojtkowski, Maciej and Szkulmowski, Maciej and Szkulmowska, Anna and Huber, Robert and Kowalczyk, Andrzej},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = mar,
      Number                   = {6},
      Pages                    = {4163 -- 4176 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.004163},
      Institution              = {Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5/7, 87-100 Torun, Poland.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-6-4163}
    }
  • Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G: In vivo endomicroscopy using three-dimensional optical coherence tomography and Fourier domain mode locked lasers. in Biomedical Optics (BiOS) 2008, pp. 684708-684708-7, International Society for Optics and Photonics, feb,, 2008
    BibTeX Link
    @InProceedings{HU_2008_Adler_b,
      Title                    = {{In vivo endomicroscopy using three-dimensional optical coherence tomography and Fourier domain mode locked lasers}},
      Author                   = {Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G},
      Booktitle                = {Biomedical Optics (BiOS) 2008},
      Year                     = {2008},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {684708--684708--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.761850},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1326810}
    }
    
  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Palte, Gesa and Huber, Robert: K-space linear Fourier domain mode locked laser and applications for optical coherence tomography. Optics Express, no. 16, pp. 8916 - 8937, OSA, jun,, 2008
    BibTeX Link
    @Article{HU_2008_Eigenwillig_b,
      Title                    = {{K-space linear Fourier domain mode locked laser and applications for optical coherence tomography}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Palte, Gesa and Huber, Robert},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = jun,
      Number                   = {12},
      Pages                    = {8916 -- 8937 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.008916},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=oe-16-12-8916}
    }
  • Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert: Optical coherence tomography imaging with k-space linear Fourier Domain Mode Locked lasers. in 2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS, 2008
    BibTeX Link
    @InProceedings{HU_2008_Eigenwillig_a,
      Title                    = {{Optical coherence tomography imaging with k-space linear Fourier Domain Mode Locked lasers}},
      Author                   = {Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert},
      Booktitle                = {2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS},
      Year                     = {2008},
    
      Url                      = {http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=4571261&searchWithin%3Deigenwillig%26sortType%3Dasc_p_Sequence%26filter%3DAND%28p_IS_Number%3A4571172%29}
    }
  • Adler, Desmond C and Huang, Shu-Wei and Huber, Robert and Fujimoto, James G: Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography. Optics Express, no. 16, pp. 4376 - 4393, mar,, 2008
    BibTeX Link
    @Article{HU_2008_Adler_a,
      Title                    = {{Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography}},
      Author                   = {Adler, Desmond C and Huang, Shu-Wei and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = mar,
      Number                   = {7},
      Pages                    = {4376 -- 4393 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.004376},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-7-4376}
    }
  • Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert: Raman-pumped Fourier-domain mode-locked laser: analysis of operation and application for optical coherence tomography. Optics Letters, no. 33, pp. 2815 - 2817, OSA, nov,, 2008
    BibTeX Link
    @Article{HU_2008_Klein_a,
      Title                    = {{Raman-pumped Fourier-domain mode-locked laser: analysis of operation and application for optical coherence tomography}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2008},
    
      Month                    = nov,
      Number                   = {23},
      Pages                    = {2815 -- 2817 },
      Volume                   = {33},
    
      Doi                      = {10.1364/OL.33.002815},
      ISSN                     = {0146-9592},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-33-23-2815}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Palte, Gesa and Adler, Desmond C and Srinivasan, Vivek J and Fujimoto, James G and Huber, Robert: Real time en face Fourier-domain optical coherence tomography with direct hardware frequency demodulation. Optics Letters, no. 33, pp. 2556 - 2558, oct,, 2008
    BibTeX Link
    @Article{HU_2008_Biedermann_a,
      Title                    = {{Real time en face Fourier-domain optical coherence tomography with direct hardware frequency demodulation}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Palte, Gesa and Adler, Desmond C and Srinivasan, Vivek J and Fujimoto, James G and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2008},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {2556 -- 2558 },
      Volume                   = {33},
    
      Doi                      = {10.1364/OL.33.002556},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-33-21-2556}
    }
  • Srinivasan, Vivek J and Adler, Desmond C and Chen, Yueli and Gorczynska, Iwona and Huber, Robert and Duker, Jay S and Schuman, Joel S and Fujimoto, James G: Ultrahigh-speed optical coherence tomography for three-dimensional and en face imaging of the retina and optic nerve head.. Investigative ophthalmology &amp; visual science, no. 49, pp. 5103-10, nov,, 2008
    BibTeX Link
    @Article{HU_2008_Srinivasan_a,
      Title                    = {{Ultrahigh-speed optical coherence tomography for three-dimensional and en face imaging of the retina and optic nerve head.}},
      Author                   = {Srinivasan, Vivek J and Adler, Desmond C and Chen, Yueli and Gorczynska, Iwona and Huber, Robert and Duker, Jay S and Schuman, Joel S and Fujimoto, James G},
      Journal                  = {Investigative ophthalmology \& visual science},
      Year                     = {2008},
    
      Month                    = nov,
      Number                   = {11},
      Pages                    = {5103--10},
      Volume                   = {49},
    
      Doi                      = {10.1167/iovs.08-2127},
      ISSN                     = {1552-5783},
      Url                      = {http://www.iovs.org/content/49/11/5103}
    }

2007

  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.: Mechanisms of laser-induced dissection and transport of histologic specimens.. Biophys J, no. 93, pp. 4481-4500, 2007
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.},
       title = {Mechanisms of laser-induced dissection and transport of histologic specimens.},
       journal = {Biophys J},
       volume = {93},
       pages = {4481-4500},
       year = {2007}
    }
  • Quaden, R. and Klaws, G. R. and Theisen-Kunde, D. and Lutter, G.: Percutaneous aortic valve replacement: first endovascular resection of human aortic valves in situ. Eur J Cardiothorac Surg, no. 31, pp. 305, 2007
    BibTeX
    @article{Quaden,
       author = {Quaden, R. and Klaws, G. R. and Theisen-Kunde, D. and Lutter, G.},
       title = {Percutaneous aortic valve replacement: first endovascular resection of human aortic valves in situ},
       journal = {Eur J Cardiothorac Surg},
       volume = {31},
       number = {2},
       pages = {305},
       note = {1010-7940 (Print)
    Journal Article},
       keywords = {Aortic Valve/*surgery
    Endoscopy
    Heart Valve Prosthesis Implantation/*methods
    Humans
    Laser Surgery/methods
    Surgical Procedures, Minimally Invasive/*methods},
       year = {2007}
    }
  • Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.: Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues. in The Eigth International Conference on Laser Ablation (COLA 2005), no. 59, pp. 249-254, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues},
       booktitle = {The Eigth International Conference on Laser Ablation (COLA 2005)},
       editor = {Herman, P. and Hess, W .},
       series = {Journal of Physics: Conference Series},
       volume = {59},
       pages = {249-254},
       year = {2007}
    }
  • Fritz, Andreas and Ptaszynski, Lars and Stoehr, Hardo and Brinkmann, Ralf: Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE). no. 6632, pp. 66321C-66321C-11,
    BibTeX Link
    @inproceedings{Fritz2007,
       author = {Fritz, Andreas and Ptaszynski, Lars and Stoehr, Hardo and Brinkmann, Ralf},
       title = {Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)},
       volume = {6632},
       pages = {66321C-66321C-11},
       note = {10.1117/12.728344},
       abstract = {Selective Retina Treatment (SRT) is a new method to treat eye diseases associated with disorders of the RPE. Selective RPE cell damage is achieved by applying a train of 1.7 μs laser pulses at 527 nm. The treatment of retinal diseases as e.g. diabetic maculopathy (DMP), is currently investigated within clinical studies, however 200 ns pulse durations are under investigation. Transient micro bubbles in the retinal pigment epithelium (RPE) are expected to be the origin of cell damage due to irradiation with laser pulses shorter than 50 μs. The bubbles emerge at the strongly absorbing RPE melanosomes. Cell membrane disruption caused by the transient associated volume increase is expected to be the origin of the angiographically observed RPE leakage. We investigate micro bubble formation and dynamics in porcine RPE using pulse durations of 150 ns. A laser interferometry system at 830 nm with the aim of an online dosimetry control for SRT was developed. Bubble formation was detected interferometrically and by fast flash photography. A correlation to cell damage observed with a vitality stain is found. A bubble detection algorithm is presented.},
       url = {http://dx.doi.org/10.1117/12.728344},
       type = {Conference Proceedings}
    }
    
  • Herrmann, Katharina and Flöhr, Christian and Stalljohann, Jens and Apiou-Sbirlea, Gabriela and Kandulla, Jochen and Birngruber, Reginald and Brinkmann, Ralf: Influence of choroidal perfusion on retinal temperature increase during retinal laser treatments. no. 6632, pp. 66321D-66321D-7,
    BibTeX Link
    @inproceedings{Herrmann2007,
       author = {Herrmann, Katharina and Flöhr, Christian and Stalljohann, Jens and Apiou-Sbirlea, Gabriela and Kandulla, Jochen and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Influence of choroidal perfusion on retinal temperature increase during retinal laser treatments},
       volume = {6632},
       pages = {66321D-66321D-7},
       note = {10.1117/12.728222},
       abstract = {In most retinal laser treatments the therapeutic effect is initiated by a transient temperature increase at and around the retinal pigment epithelium (RPE). Especially in long exposure time treatments like Transpupillary Thermotherapy (TTT) choroidal perfusion has a strong influence on the realized temperature at the fundus. The fundus blood circulation and therefore the heat dissipation is influenced by the intraocular pressure (IOP), which is investigated in the study presented here. In order to reduce the choroidal perfusion, the IOP is increased by injection of physiological saline solution into the eye of anaesthetized rabbits. The fundus is irradiated with 3.64 W/cm2 by means of a TTT-laser (λ = 810 nm) for t = 20 s causing a retinal temperature increase. Realtime temperature determination at the irradiated spot is achieved by a non invasive optoacoustic technique. Perfusion can be reduced by increasing IOP, which leads to different temperature increases when irradiating the retina. This should be considered for long time laser treatments.},
       url = {http://dx.doi.org/10.1117/12.728222},
       type = {Conference Proceedings}
    }
    
  • Steven, P. and Rupp, J. and Huettmann, G. and Koop, N. and Laqua, H.: Two-Photon Real-Time Imaging of Conjunctiva-Associated Lymphoid Tissue (CALT). Investigative Ophthalmology & Visual Science, no. 48, pp. 201-201, 2007
    BibTeX Link
    @article{Steven2007,
       author = {Steven, P. and Rupp, J. and Huettmann, G. and Koop, N. and Laqua, H.},
       title = {Two-Photon Real-Time Imaging of Conjunctiva-Associated Lymphoid Tissue (CALT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {48},
       number = {13},
       pages = {201-201},
       abstract = {AbstractPurpose:: Immunological real-time analysis of conjunctiva-associated lymphoid tissue (CALT) is challenging at state. For the first time, two-photon microscopy, a new optical method, is evaluated in its use to analyze morphology and function of CALT. Methods:: Conjunctiva of female Balb/c mice is challenged with Chlamydia trachomatis serovar C (ChtC) or ovalbumin and choleratoxin subunit B (OVA/CTB) for CALT induction. A two-photon microscope equipped with a near infrared femtosecond-laser and a fluorescence-lifetime detector is used for ex-vivo analysis of unfixed and unstained ocular tissue with additional application of fluorescent microspheres to demonstrate transepithelial particle transport. Results:: Challenge with ChtC or OVA/CTB induce all CALT components (lymphoepithelium, follicles, blood and lymphatic vessels), that are demonstrated in cellular and subcellular resolution by means of autofluorescence imaging. Wavelength adaptation allows specific differentiation of cellular and acellular components. Fluorescence-lifetime detection permits differentiation of cellular subsets (e.g. lymphocytes and macrophages). Application of fluorescent microspheres demonstrates transepithelial particle transport and detection within intracellular vesicles. Conclusions:: Two-photonmicroscopy is an innovative optical technique to analyse morphological and functional features of CALT. Detection of transepithelial particle transport and its impact on conjunctival immunological processes can be visualized in real-time. Future in-vivo experiments with suitable animal models would allow detailed analysis of CALT in a clinical context e.g. corneal transplant rejection, keratoconjunctivitis sicca and follicular conjunctivitis.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2007},
       type = {Journal Article}
    }
    
  • Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther: Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues. in Laser Ablation and Its Applications, no. 129, pp. 231-280, Springer US, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther},
       title = {Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues},
       booktitle = {Laser Ablation and Its Applications},
       editor = {Phipps, Claude},
       series = {Springer Series in Optical Sciences},
       publisher = {Springer US},
       volume = {129},
       chapter = {10},
       pages = {231-280},
       year = {2007}
    }
    
  • Theisen-Kunde, D and Ott, V and Brinkmann, R and Keller, R: Potential of a new cw 2µm laser scalpel for laparoscopic surgery. Med. Laser Appl., no. 22, pp. 139-45, 2007
    BibTeX
    @article{Theisen-Kunde,
       author = {Theisen-Kunde, D and Ott, V and Brinkmann, R and Keller, R},
       title = {Potential of a new cw 2µm laser scalpel for laparoscopic surgery},
       journal = {Med. Laser Appl.},
       volume = {22},
       number = {2},
       pages = {139-45},
       abstract = {Abstract
    The potential of a new continuous-wave (cw) thulium–YAG laser for laparoscopic small intestine resection was investigated in pigs in comparison to standard bipolar scissors.
    
    Method
    A diode-pumped solid-state thulium–YAG laser system emitting at a wavelength of 2.01 µm was used. Laser power up to 25 W in cw mode was transmitted via a quartz fibre (400 µm core diameter). In order to resect 1 m of the small intestine, the accompanied mesentery was dissected with both devices in 12 pigs (six each group). Arteries and veins of 0.25–3.2 mm inner diameter were dissected in vivo and the resistance of the occluded vessels to pressures up to 375 mmHg was measured by an in vitro set-up. Samples were prepared for histological evaluation.
    
    Results
    With respect to intestine resection with bipolar scissors, bleeding occurred significantly less (25%) and dissection time was reduced by 19% using the 2 µm laser scalpel. With the 2 µm laser scalpel, small vessels (<0.5 mm) were successfully occluded up to 100% (arteries) and 89% (veins), larger vessels (1–2.3 mm) to 74% (arteries) and 65% (veins) in vivo. In the in vitro pressure measurement with 375 mmHg, 30% of veins and 35% of arteries stayed closed. In conclusion, the first experiments show that the 2 µm laser scalpel is a promising dissection device for minimally invasive surgery. 
    Zusammenfassung
    In einer vergleichenden Tierstudie wurde das Potential eines neuen cw Thulium–YAG-Lasersystems mit dem einer kommerziell erhältlichen bipolaren Schere zur laparoskopischen Resektion des Dünndarms verglichen.
    
    Methode
    Es wurde ein diodengepumptes Thulium–YAG-Festkörper-Lasersystem mit einer Emissionswellenlänge von 2,01 µm verwendet. Die Laserleistung betrug 25 W (Dauerstrich) und die Laserstrahlung wurde mittels einer Quarzglasfaser (Kerndurchmesser 400 µm) zum Applikationsort transmittiert. Zur Entnahme von 1 m Dünndarm wurde zunächst mit beiden Dissektionsgeräten das angrenzende Mesenterium an 12 Schweinen (6 je Instrument) durchtrennt. Zur Untersuchung der Hämostaseeigenschaften des 2 µm Laserskalpells wurden ausgewählte Arterien und Venen mit einem inneren Durchmesser von 0,25 bis 3,2 mm in vivo durchtrennt. In einer anschließenden in vitro Untersuchung wurde der Gefäßverschluss mit einem Druck von 375 mmHg überprüft. Zur histologischen Auswertung wurden Gewebeproben entnommen, mittels H&E gefärbt und lichtmikroskopisch untersucht.
    
    Ergebnisse
    Im Vergleich zur konventionellen bipolaren Schere konnte in der durchgeführten Studie die Anzahl der auftretenden Blutungen mit dem 2 µm Laserskalpell signifikant (25%) und die gesamte Resektionszeit um 19% (nicht signifikant) reduziert werden. Die Hämostase von Gefäßen, welche mittels des 2 µm Laserskalpells durchtrennt wurden, betrug bei Durchmessern <0.5 mm 100% bei den Arterien und 89% bei den Venen. Bei größeren Gefäßen (1–2.3 mm innerer Durchmesser) wurden 74% der Arterien und 65% der Venen dauerhaft in vivo koaguliert. Bei den in vitro Untersuchungen der koagulierten Gefäße mit einem Druck von 375 mmHg waren noch insgesamt 35% der Arterien und 30% der Venen verschlossen.
    
    In dieser Studie konnte gezeigt werden, dass das 2 µm Laserskalpell ein vielversprechendes Instrument für die laparoskopische Chirurgie darstellt.
    
    Resúmen
    En este estudio se investigó el potencial del nuevo láser cw thulium–YAG comparado al uso de la tijera bipolar estándar en intervenciones laparoscópicas en intestino delgado de cerdos.
    
    Método
    Se utilizó un láser thulium–YAG de estado sólido bombeado por diodos que emite en una longitud de onda de 2.01 µm. Una potencia láser de hasta 25 W de onda continua (cw) fue trasmitida a través de una fibra de cuarzo de 400 µm de diámetro. El mesenterio que acompaña al intestino delgado fue diseccionado utilizando uno de los dos sistemas en 12 cerdos (6 con cada instrumento). Se seccionaron in vivo, arterias y venas de 0.25 a 3.2 mm de diámetro interno y se midió in vitro la resistencia de los vasos ocluídos con una presión de hasta 375 mmHg. Las muestras fueron preparadas luego para evaluaciones histológicas.
    
    Resultados
    En la resección de intestino, el uso del bisturí láser de 2 µm ofrece menor sangrado (25%) y el reduce el tiempo de dissección en un 19% con respecto a las tijeras bipolares. Además, el uso del láser permite la oclusión in vivo de los pequeños vasos (diámetro menor a 0,5 mm) hasta en un 100% en arterias y un 89% en venas. Mientras que para las de mayor diámetro (de 1 a 2.3 mm) se obtuvo un 74% y 65%, respectivamente. En las mediciones in vitro de presión con 375 mmHg, un 30% de las venas y un 35% de las arterias permanecieron cerradas. En conclusión, estos primeros experimentos demuestran que el bisturí láser de 2 µm es una herramienta de disección prometedora para la cirugía laparoscópica. 
    
    Keywords: Thulium laser; Minimally invasive; Vessel sealing; Laser scalpel
    Schlüsselwörter: Thuliumlaser; Minimalinvasiv; Hämostase; Laserskalpell
    Palabras clave: Thulium láser; Cirugía mínimamente invasiva; Oclusión de vasos; Bisturí láser},
       year = {2007}
    }
  • Brinkmann, R. and Birngruber, R.: Selektive Retina-Therapie (SRT). Zeitschrift für Medizinische Physik, no. 17, pp. 6-22, 2007
    BibTeX Link
    @article{Brinkmann2007,
       author = {Brinkmann, R. and Birngruber, R.},
       title = {Selektive Retina-Therapie (SRT)},
       journal = {Zeitschrift für Medizinische Physik},
       volume = {17},
       number = {1},
       pages = {6-22},
       note = {276HP
    Times Cited:0
    Cited References Count:42},
       abstract = {Selective Retina Therapy (SRT) is a new and very gentle laser method developed at the Medical Laser Center Lubeck. It is currently investigated clinically in order to treat retinal disorders associated with a decreased function of the retinal pigment epithelium (RPE). SRT is designed to selectively effect the RPE while sparing the neural retina and the photoreceptors as well as the chorioidea. Aim of the therapy is the rejuvenation of the RPE in the treated areas, which should ideally lead to a long term metabolic increase at the chorio- retinal junction. In contrast to conventional laser photocoagulation, which is associated with a complete thermal necrosis of the treated site, SRT completely retains full vision. This paper reviews the methods and mechanisms behind selective RPE effects and reports the first clinical results. An online dosimetry technique to visualize the ophthalmoscopically invisible effects is introduced.},
       keywords = {selective cellular effects
    optoacoustics
    online dosimetry
    rpe
    mu s-laser pulses
    macula oedema
    rcs
    pigment epithelium
    diabetic maculopathy
    laser irradiation
    time regimen
    damage
    rpe
    photocoagulation
    absorption
    mechanisms
    radiation},
       ISSN = {0939-3889},
       DOI = {DOI 10.1016/j.zemedi.2006.11.002},
       url = {<Go to ISI>://WOS:000254132200002},
       year = {2007},
       type = {Journal Article}
    }
    
  • Schlott, Kerstin and Stalljohann, Jens and Weber, Benjamin and Kandulla, Jochen and Herrmann, Katharina and Birngruber, Reginald and Brinkmann, Ralf: Optoacoustic online temperature determination during retinal laser photocoagulation. no. 6632, pp. 66321B-66321B-8,
    BibTeX Link
    @inproceedings{Schlott2007,
       author = {Schlott, Kerstin and Stalljohann, Jens and Weber, Benjamin and Kandulla, Jochen and Herrmann, Katharina and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Optoacoustic online temperature determination during retinal laser photocoagulation},
       volume = {6632},
       pages = {66321B-66321B-8},
       note = {10.1117/12.728291},
       abstract = {Retinal photocoagulation is an established treatment of different retinal diseases. The treatment relies on a short, local heating of the tissue which induces a denaturation. The resulting scar formation may for example prevent the further detachment of the retina. The extent of the coagulation is besides other parameters mostly dependent on the induced temperature increase. However, until today a temperature based dosimetry for photocoagulation does not exist. The dosage is rather based on the experience of the treating physicians to achieve visible whitish lesions on the retina. In this work a technique is presented, which allows an online temperature monitoring during photocoagulation. If an absorbing material is irradiated with short laser pulses, a thermoelastic expansion of the absorber induces an acoustic wave. Its amplitude is dependent on the temperature of the absorber. For analyzing the applicability of the optoacoustic temperature determination for dosimetry, measurements were performed on enucleated porcine eye globes. The pressure transients are detected by an ultrasonic transducer, which is embedded in an ophthalmologic contact lens. As long as no strong lesions occur, the determined temperatures are almost proportional to the power of the treatment laser. Using a spot diameter of 200 μm and different laser powers, the temperature rise at the end of the 400 ms irradiation was found to be approximately 0.16 °C/mW. The onset of the denaturation was observed around 50°C. The far aim of this project is an automatic regulation of the treatment laser onto a desired temperature course.},
       url = {http://dx.doi.org/10.1117/12.728291},
       type = {Conference Proceedings}
    }
    
  • Stoehr, Hardo and Ptaszynski, Lars and Fritz, Andreas and Brinkmann, Ralf: Interferometric optical online dosimetry for selective retina treatment (SRT). no. 6426, pp. 642619-642619-7,
    BibTeX Link
    @inproceedings{Stoehr2007,
       author = {Stoehr, Hardo and Ptaszynski, Lars and Fritz, Andreas and Brinkmann, Ralf},
       title = {Interferometric optical online dosimetry for selective retina treatment (SRT)},
       volume = {6426},
       pages = {642619-642619-7},
       note = {10.1117/12.708521},
       abstract = {In selective retina treatment (SRT) spatial confined tissue damage in the absorbing retinal pigment epithelium (RPE) is obtained by applying microsecond laser pulses. The damage in the RPE is caused by transient microbubbles forming around the laser heated melanin granules inside the cells. For treatment of RPE related diseases, SRT is thought to share the therapeutic benefits of conventional photocoagulation but without affecting the photoreceptors. A drawback for effective clinical SRT is that the laser-induced lesions are ophthalmoscopically invisible. Therefore, a real-time feedback system for dosimetry is demanded in order to avoid undertreatment or unwanted collateral damage to the adjacent tissue. We develop a dosimetry system which uses optical interferometry for the detection of the transient microbubbles. The system is based on an optical fiber interferometer which is operated with a laser diode at 830nm. We present current results obtained with porcine RPE explants in vitro and complete porcine eye globes ex vivo.},
       url = {http://dx.doi.org/10.1117/12.708521},
       type = {Conference Proceedings}
    }
    
  • Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther: Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues Laser Ablation and its Applications. no. 129, pp. 231-280, Springer Berlin / Heidelberg, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther},
       title = {Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues
    Laser Ablation and its Applications},
       editor = {Phipps, Claude},
       series = {Springer Series in Optical Sciences},
       publisher = {Springer Berlin / Heidelberg},
       volume = {129},
       pages = {231-280},
       keywords = {Physics and Astronomy},
       year = {2007}
    }
  • Framme, C. and Alt, C. and Schnell, S. and Sherwood, M. and Brinkmann, R. and Lin, C. P.: Selective targeting of the retinal pigment epithelium in rabbit eyes with a scanning laser beam. Invest Ophthalmol Vis Sci, no. 48, pp. 1782-92, 2007
    BibTeX
    @article{Framme,
       author = {Framme, C. and Alt, C. and Schnell, S. and Sherwood, M. and Brinkmann, R. and Lin, C. P.},
       title = {Selective targeting of the retinal pigment epithelium in rabbit eyes with a scanning laser beam},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {48},
       number = {4},
       pages = {1782-92},
       note = {0146-0404 (Print)
    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't},
       abstract = {PURPOSE: Selective targeting of the retinal pigment epithelium (RPE) with repetitive laser pulses that minimize thermal damage to the adjacent photoreceptors is a promising new therapeutic modality for RPE-related retinal diseases. The selectivity of an alternative, more versatile scanning approach was examined in vivo by using a broad range of scanning parameters. METHODS: Acousto-optic deflectors repeatedly scanned the focus of a continuous wave (cw)-laser across the retina of Dutch belted rabbits, producing microsecond irradiation at each RPE cell. Two irradiation patterns forming separated lines (SEP) or interlaced lines (INT), different dwell times (2.5-75 micros), and repetition numbers (10 and 100 scans with 100-Hz repetition rate) were tested. Thresholds were evaluated by fundus imaging and angiography. Histology was performed for selected parameters. RESULTS: Selective RPE cell damage was obtained with moderate laser power. The angiographic threshold power decreased with pulse duration, number of exposures, and applying the INT pattern. Ophthalmoscopic thresholds, indicating onset of thermal coagulation, were higher than twice the angiographic threshold for most tested parameters. Histology confirmed selective RPE cell damage for SEP irradiation with 7.5 and 15 micros; slower scan speeds or closed lines caused photoreceptor damage. CONCLUSIONS: A cw-laser scanner can be set up as a highly compact and versatile device. Selective RPE damage is feasible with dwell times up to 15 micros. Greatest selectivity is achieved with short exposure times and separated scan lines. Interlaced lines and long exposure times facilitate heat conduction into photoreceptors. A scanner is an attractive alternative for pulsed selective targeting, because both selective targeting and thermal photocoagulation can be realized.},
       keywords = {Animals
    Eye Injuries/diagnosis
    Fluorescein Angiography
    Laser Coagulation/adverse effects/instrumentation/*methods
    Ophthalmoscopy
    Photoreceptors, Vertebrate/pathology
    Pigment Epithelium of Eye/injuries/pathology/*surgery
    Rabbits
    Retina/injuries/pathology},
       year = {2007}
    }
  • Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.: Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif, no. 40, pp. 422-30, 2007
    BibTeX
    @article{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.},
       title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis},
       journal = {Cell Prolif},
       volume = {40},
       number = {3},
       pages = {422-30},
       note = {Rahmanzadeh, R
    Huttmann, G
    Gerdes, J
    Scholzen, T
    England
    Cell Prolif. 2007 Jun;40(3):422-30.},
       abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.},
       keywords = {Antibodies, Antinuclear
    Antibodies, Monoclonal
    Cell Division/physiology
    Cell Nucleolus/physiology
    Fluorescein-5-isothiocyanate
    Fluorescent Dyes
    HeLa Cells
    Humans
    Ki-67 Antigen/*genetics/*metabolism
    Photochemistry
    RNA Polymerase I/metabolism
    RNA, Ribosomal/*biosynthesis},
       year = {2007}
    }
  • Vogel, Alfred and Apitz, Ingo and Venugopalan, Vasan: Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues. in Oscillations, Waves and Interactions, T. Kurz, U. Parlitz, and U. Kaatze, eds.(Universitätsverlag Göttingen, Göttingen, 2007), pp. 217-258, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Apitz, Ingo and Venugopalan, Vasan},
       title = {Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues},
       booktitle = {Oscillations, Waves and Interactions, T. Kurz, U. Parlitz, and U. Kaatze, eds.(Universitätsverlag Göttingen, Göttingen, 2007)},
       pages = {217-258},
       year = {2007}
    }
    
  • Neumann, J and Brinkmann, R: Nucleation dynamics around single microabsorbers in water heated by nanosecond irradiation. J Appl Phys, no. 101, 2007
    BibTeX
    @article{Neumann,
       author = {Neumann, J and Brinkmann, R},
       title = {Nucleation dynamics around single microabsorbers in water heated by nanosecond irradiation},
       journal = {J Appl Phys},
       volume = {101},
       number = {114701},
       year = {2007}
    }
  • Brinkmann, R. and Birngruber, R.: [Selective Retina Therapy (SRT)]. Z Med Phys, no. 17, pp. 6-22, 2007
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R. and Birngruber, R.},
       title = {[Selective Retina Therapy (SRT)]},
       journal = {Z Med Phys},
       volume = {17},
       number = {1},
       pages = {6-22},
       note = {Brinkmann, Ralf
    Birngruber, Reginald
    English Abstract
    Review
    Germany
    Z Med Phys. 2007;17(1):6-22.},
       abstract = {Selective Retina Therapy (SRT) is a new and very gentle laser method developed at the Medical Laser Center Lubeck. It is currently investigated clinically in order to treat retinal disorders associated with a decreased function of the retinal pigment epithelium (RPE). SRT is designed to selectively effect the RPE while sparing the neural retina and the photoreceptors as well as the chorioidea. Aim of the therapy is the rejuvenation of the RPE in the treated areas, which should ideally lead to a long term metabolic increase at the chorio-retinal junction. In contrast to conventional laser photocoagulation, which is associated with a complete thermal necrosis of the treated site, SRT completely retains full vision. This paper reviews the methods and mechanisms behind selective RPE effects and reports the first clinical results. An online dosimetry technique to visualize the ophthalmoscopically invisible effects is introduced.},
       keywords = {Humans
    Laser Therapy
    Pigment Epithelium of Eye/pathology
    Regeneration
    Retinal Diseases/pathology/surgery/ therapy},
       year = {2007}
    }
    
  • Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.: Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography. Curr Eye Res, no. 32, pp. 171-176, 2007
    BibTeX
    @article{Müller,
       author = {Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.},
       title = {Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography},
       journal = {Curr Eye Res},
       volume = {32},
       pages = {171-176},
       year = {2007}
    }
  • Brinkmann, R and Stalljohann, J and Weber, B and Schlott, K and Kandulla, J and Birngruber, R: Retinal Temperature Determination During Laser Photocoagulation. Proc SPIE, no. 6632, pp. 8, 2007
    BibTeX
    @article{Brinkmann2007,
       author = {Brinkmann, R and Stalljohann, J and Weber, B  and Schlott, K and Kandulla, J and Birngruber, R},
       title = {Retinal Temperature Determination During Laser Photocoagulation},
       journal = {Proc SPIE},
       volume = {6632},
       pages = {8},
       year = {2007},
       type = {Journal Article}
    }
    
  • Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.: Optische Kohärenztomographie in der Gynokologie. Der Gynäkologe, no. 40, 2007
    BibTeX
    @article{Löning,
       author = {Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.},
       title = {Optische Kohärenztomographie in der Gynokologie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       year = {2007}
    }
  • Hüttmann, G.: Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie. Der Gynäkologe, no. 40, pp. 372, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G.},
       title = {Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {372},
       year = {2007}
    }
  • Qu, Xiaochao and Norbert, Koop and Li, Zheng and Wang, Jing and Zhang, Zhenxi and Hüttmann, Gereon: Multiphoton fluorescence lifetime imaging of Karpas 299 cells using ACT1 antibody conjugated gold nanoparticles. no. 6630, pp. 66301C-66301C-8,
    BibTeX Link
    @inproceedings{Qu2007,
       author = {Qu, Xiaochao and Norbert, Koop and Li, Zheng and Wang, Jing and Zhang, Zhenxi and Hüttmann, Gereon},
       title = {Multiphoton fluorescence lifetime imaging of Karpas 299 cells using ACT1 antibody conjugated gold nanoparticles},
       volume = {6630},
       pages = {66301C-66301C-8},
       note = {10.1117/12.728239},
       abstract = {Due to the unique optical properties, gold nanoparticles (NPs) can play a useful role in biological cellular imaging as biological probes. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) system, we recorded the images of Karpas 299 cells incubated without, or with gold NPs, and ACT1 antibodies conjugated with gold NPs. From the FLIM, we can easily discriminate the difference among different experiment conditions due to the distinct lifetime between cells and gold NPs. Our results present that nonconjugated gold NPs are accumulated inside cells, but conjugated gold NPs bind homogeneously and specifically to the surface of cancer cells. For single Karpas 299 cells, the signal is very week when the excitation power is about 10mw; while the power is approximately 28 mw, a very sharp cell imaging can be obtained. For the Karpas 299 incubated with ACT1 conjugated gold NPs, while the excitation power is 10mw, gold NPs have clear fluorescence signal so that the profile of cells can be detected; Signal of gold NPs is very strong when the power arrived in 20mw. These results suggest that the multiphoton lifetime imaging of antibody conjugated gold NPs can support a useful method in diagnosis of cancer.},
       url = {http://dx.doi.org/10.1117/12.728239},
       type = {Conference Proceedings}
    }
    
  • Rusanov, V. and Paulsen, H. and Böttger, L. H. and Winkler, H. and Wolny, J. A. and Koop, N. and Dorn, Th. and Janiak, C. and Trautwein, A. X.: Mössbauer, nuclear inelastic scattering and density functional studies on the second metastable state of Na2[Fe(CN)5NO]·2H2O. Hyperfine Interactions, no. 175, pp. 141-150, 2007
    BibTeX Link Link
    @article{Rusanov2007,
       author = {Rusanov, V. and Paulsen, H. and Böttger, L. H. and Winkler, H. and Wolny, J. A. and Koop, N. and Dorn, Th. and Janiak, C. and Trautwein, A. X.},
       title = {Mössbauer, nuclear inelastic scattering and density functional studies on the second metastable state of Na2[Fe(CN)5NO]·2H2O},
       journal = {Hyperfine Interactions},
       volume = {175},
       number = {1},
       pages = {141-150},
       abstract = {The structure of the light-induced metastable state SII of Na2[Fe(CN)5NO]·2H2O was investigated by transmission Mössbauer spectroscopy (TMS) in the temperature range between 85 and 135 K, nuclear inelastic scattering (NIS) at 98 K using synchrotron radiation and density functional theory (DFT) calculations. The DFT and TMS results strongly support the view that the NO group in SII takes a side-on molecular orientation and, further, is dynamically displaced from one eclipsed, via a staggered, to a second eclipsed orientation. The population conditions for generating SII are optimal for measurements by TMS, yet they are modest for accumulating NIS spectra. Optimization of population conditions for NIS measurements is discussed and new NIS experiments on SII are proposed.},
       ISSN = {1572-9540},
       DOI = {10.1007/s10751-008-9598-8},
       url = {http://dx.doi.org/10.1007/s10751-008-9598-8},
       year = {2007},
       type = {Journal Article}
    }
    
  • Hüttmann, G. and Löning, M.: Physikalische Grundlagen optischer Technologien. Der Gynäkologe, no. 40, pp. 332-337, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G. and Löning, M.},
       title = {Physikalische Grundlagen optischer Technologien},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {332-337},
       year = {2007}
    }
  • Vogel, A and Apitz, I. and Venugopalan, V.: Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues. in Oscillations, Waves, and Interactions. Sixty Years Drittes Physikalisches Institut., no. A Festschrift, pp. 217-258, Universitätsverlag, Göttingen, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Apitz, I. and Venugopalan, V.},
       title = {Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues},
       booktitle = {Oscillations, Waves, and Interactions. Sixty Years Drittes Physikalisches Institut.},
       editor = {Kurtz, T., Parlitz, U. and  Kaatze, U.},
       publisher = {Universitätsverlag},
       address = {Göttingen},
       volume = {A Festschrift},
       pages = {217-258},
       year = {2007}
    }
  • Framme, C and Brinkmann, R: Die Selektive Retina Therapie (SRT). Der Augenspiegel, no. 53G1396, 2007
    BibTeX
    @article{Framme,
       author = {Framme, C and Brinkmann, R},
       title = {Die Selektive Retina Therapie (SRT)},
       journal = {Der Augenspiegel},
       volume = {53G1396},
       number = {11},
       year = {2007}
    }
  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Horneffer, V. and Linz, N. and Vogel, A.: Principles of laser-induced separation and transport of living cells. J Biomed Opt, no. 12, pp. 054016, 2007
    BibTeX
    @article{Horneffer,
       author = {Horneffer, V. and Linz, N. and Vogel, A.},
       title = {Principles of laser-induced separation and transport of living cells},
       journal = {J Biomed Opt},
       volume = {12},
       number = {5},
       pages = {054016},
       note = {Horneffer, Verena
    Linz, Norbert
    Vogel, Alfred
    Evaluation Studies
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2007 Sep-Oct;12(5):054016.},
       abstract = {Separation and transport of defined populations of living cells grown on a thin membrane can be achieved by laser microdissection (LMD) of the sample of interest, followed by a laser-induced forward transport process [laser pressure "catapulting" (LPC)] of the dissected cell cluster. We investigate the dynamics of LMD and LPC with focused and defocused UV-A laser pulses by means of time-resolved photography. Catapulting is driven by plasma formation when tightly focused pulses are used, and by confined thermal ablation at the bottom of the sample for defocused catapulting. With both modalities, the initial specimen velocity amounts to about 50 to 60 ms. Time-resolved photography of live cell catapulting reveals that in defocused catapulting, strong shear forces arise when the sample is accelerated out of the culture medium covering the cells. By contrast, pulses focused at the periphery of the specimen cause a fast rotational movement that minimizes the flow of culture medium parallel to the sample surface, and thus the resulting shear stresses. Therefore, the recultivation rate of catapulted cells is much higher when focused pulses are used. Compared to collateral damage by mechanical forces, side effects by heat and UV exposure of the cells play only a minor role.},
       keywords = {Animals
    CHO Cells
    Cell Separation/ methods
    Cricetinae
    Cricetulus
    Microdissection/ methods
    Optical Tweezers
    Specimen Handling/ methods},
       year = {2007}
    }
  • Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.: Combining Optical Coherence Tomographie (OCT) with an operating microscope. in Medical Engineering, pp. pp 343-348, Springer, Berlin, Heidelberg, New York, 2007
    BibTeX
    @inbook{Lankenau,
       author = {Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.},
       title = {Combining Optical Coherence Tomographie (OCT) with an operating microscope},
       booktitle = {Medical Engineering},
       editor = {Buzug, T.M. and Holz, D. and Weber, S.  and Bongartz, J. and Kohl-Bareise, M. and Hartmann, U.},
       publisher = {Springer, Berlin, Heidelberg, New York},
       pages = {pp 343-348},
       year = {2007}
    }
  • Stoehr, Hardo and Ptaszynski, Lars and Fritz, Andreas and Brinkmann, Ralf: Interferometric optical online dosimetry for selective retina treatment (SRT). no. 6426, pp. 642619-642619-7,
    BibTeX
    @inproceedings{Stoehr2007,
       author = {Stoehr, Hardo and Ptaszynski, Lars and Fritz, Andreas and Brinkmann, Ralf},
       title = {Interferometric optical online dosimetry for selective retina treatment (SRT)},
       volume = {6426},
       pages = {642619-642619-7},
       note = {10.1117/12.708521},
       abstract = {In selective retina treatment (SRT) spatial confined tissue damage in the absorbing retinal pigment epithelium (RPE) is obtained by applying microsecond laser pulses. The damage in the RPE is caused by transient microbubbles forming around the laser heated melanin granules inside the cells. For treatment of RPE related diseases, SRT is thought to share the therapeutic benefits of conventional photocoagulation but without affecting the photoreceptors. A drawback for effective clinical SRT is that the laser-induced lesions are ophthalmoscopically invisible. Therefore, a real-time feedback system for dosimetry is demanded in order to avoid undertreatment or unwanted collateral damage to the adjacent tissue. We develop a dosimetry system which uses optical interferometry for the detection of the transient microbubbles. The system is based on an optical fiber interferometer which is operated with a laser diode at 830nm. We present current results obtained with porcine RPE explants in vitro and complete porcine eye globes ex vivo.},
       url = {http://dx.doi.org/10.1117/12.708521},
       type = {Conference Proceedings}
    }
    
  • Fritz, Andreas and Ptaszynski, Lars and Stoehr, Hardo and Brinkmann, Ralf: Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE). no. 6632, pp. 66321C-66321C-11,
    BibTeX
    @inproceedings{Fritz2007,
       author = {Fritz, Andreas and Ptaszynski, Lars and Stoehr, Hardo and Brinkmann, Ralf},
       title = {Dynamics and detection of laser induced microbubbles in the retinal pigment epithelium (RPE)},
       volume = {6632},
       pages = {66321C-66321C-11},
       note = {10.1117/12.728344},
       abstract = {Selective Retina Treatment (SRT) is a new method to treat eye diseases associated with disorders of the RPE. Selective RPE cell damage is achieved by applying a train of 1.7 μs laser pulses at 527 nm. The treatment of retinal diseases as e.g. diabetic maculopathy (DMP), is currently investigated within clinical studies, however 200 ns pulse durations are under investigation. Transient micro bubbles in the retinal pigment epithelium (RPE) are expected to be the origin of cell damage due to irradiation with laser pulses shorter than 50 μs. The bubbles emerge at the strongly absorbing RPE melanosomes. Cell membrane disruption caused by the transient associated volume increase is expected to be the origin of the angiographically observed RPE leakage. We investigate micro bubble formation and dynamics in porcine RPE using pulse durations of 150 ns. A laser interferometry system at 830 nm with the aim of an online dosimetry control for SRT was developed. Bubble formation was detected interferometrically and by fast flash photography. A correlation to cell damage observed with a vitality stain is found. A bubble detection algorithm is presented.},
       url = {http://dx.doi.org/10.1117/12.728344},
       type = {Conference Proceedings}
    }
    
  • Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.: Principles of laser microdissection and catapulting of histologic specimens and live cells. in Laser Manipulation of Cells and Tissues, Methods in Cell Biology, no. 82, pp. 153-205, Academic Press Elsevier, San Diego, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.},
       title = {Principles of laser microdissection and catapulting of histologic specimens and live cells},
       booktitle = {Laser Manipulation of Cells and Tissues, Methods in Cell Biology},
       editor = {Berns, M.  and Greulich, K.O.},
       publisher = {Academic Press Elsevier},
       address = {San Diego},
       volume = {82},
       pages = {153-205},
       year = {2007}
    }
  • Theisen-Kunde, D and Tedsen, S and Danicke, V and Herrmann, K and Brinkmann, R: Partial kidney resection by use of a 1,94 µm thulium fiber laser. in Proc ECBO, no. 6632, SPIE,
    BibTeX
    @inproceedings{Theisen-Kunde2007,
       author = {Theisen-Kunde, D and Tedsen, S and Danicke, V and Herrmann, K and Brinkmann, R},
       title = {Partial kidney resection by use of a 1,94 µm thulium fiber laser},
       booktitle = {Proc ECBO},
       series = {Therapeutic Laser Applications and Laser-Tissue Interactions},
       publisher = {SPIE},
       volume = {6632},
       type = {Conference Proceedings}
    }
    
  • Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.: Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues. in Handai Nanophotonics, no. Volume 3, pp. 273-286, Elsevier, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues},
       booktitle = {Handai Nanophotonics},
       editor = {Hiroshi Masuhara, Satoshi Kawata and Fumio, Tokunaga},
       publisher = {Elsevier},
       volume = {Volume 3},
       pages = {273-286},
       year = {2007}
    }
  • Adler, Desmond C and Stenger, Jens and Gorczynska, Iwona and Lie, Henry and Hensick, Teri and Spronk, Ron and Wolohojian, Stephan and Khandekar, Narayan and Jiang, James Y and Barry, Scott and Cable, Alex E and Huber, Robert and Fujimoto, James G: Comparison of three-dimensional optical coherence tomography and high resolution photography for art conservation studies. Optics Express, no. 15, pp. 15972 -15986, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Adler_b,
      Title                    = {{Comparison of three-dimensional optical coherence tomography and high resolution photography for art conservation studies}},
      Author                   = {Adler, Desmond C and Stenger, Jens and Gorczynska, Iwona and Lie, Henry and Hensick, Teri and Spronk, Ron and Wolohojian, Stephan and Khandekar, Narayan and Jiang, James Y and Barry, Scott and Cable, Alex E and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {24},
      Pages                    = {15972 -- 15986 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.015972},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-24-15972}
    }
  • Huber, Robert and Srinivasan, Vivek J and Adler, Desmond C and Gorczynska, I and Fujimoto, James G: Fourier Domain Mode Locking (FDML) in the non-zero dispersion regime: A laser for ultrahigh-speed retinal OCT imaging at 236kHz line rate. in Conference on Lasers and Electro-Optics, 2007, CLEO 2007, 2007
    BibTeX Link
    @InProceedings{HU_2007_Huber_b,
      Title                    = {{Fourier Domain Mode Locking (FDML) in the non-zero dispersion regime: A laser for ultrahigh-speed retinal OCT imaging at 236kHz line rate}},
      Author                   = {Huber, Robert and Srinivasan, Vivek J and Adler, Desmond C and Gorczynska, I and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics, 2007, CLEO 2007},
      Year                     = {2007},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=CLEO-2007-CThAA5}
    }
  • Huber, R and Adler, D C and Srinivasan, V J and Fujimoto, J G: Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second. Optics Letters, no. 32, pp. 2049 - 2051, jul,, 2007
    BibTeX Link
    @Article{HU_2007_Huber_a,
      Title                    = {{Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second}},
      Author                   = {Huber, R and Adler, D C and Srinivasan, V J and Fujimoto, J G},
      Journal                  = {Optics Letters},
      Year                     = {2007},
    
      Month                    = jul,
      Number                   = {14},
      Pages                    = {2049 -- 2051},
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.002049},
      Institution              = {Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. Robert.Huber@Physik.Uni-Muenchen.de},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-14-2049}
    }
  • Huber, Robert A and Adler, Desmond C and Srinivasan, Vivek J and Gorczynska, Iwona M and Fujimoto, James G: Fourier domain mode-locked (FDML) lasers at 1050 nm and 202,000 sweeps per second for OCT retinal imaging. in Proceedings of SPIE, no. 6429, pp. 642907-642907-7, feb,, 2007
    BibTeX Link
    @InProceedings{HU_2007_Huber_c,
      Title                    = {{Fourier domain mode-locked (FDML) lasers at 1050 nm and 202,000 sweeps per second for OCT retinal imaging}},
      Author                   = {Huber, Robert A and Adler, Desmond C and Srinivasan, Vivek J and Gorczynska, Iwona M and Fujimoto, James G},
      Booktitle                = {Proceedings of SPIE},
      Year                     = {2007},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {642907--642907--7},
      Volume                   = {6429},
    
      Doi                      = {10.1117/12.704084},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1296450}
    }
  • Kranendonk, Laura A and An, Xinliang and Caswell, Andrew W and Herold, Randy E and Sanders, Scott T and Huber, Robert and Fujimoto, James G and Okura, Yasuhiro and Urata, Yasuhiro: High speed engine gas thermometry by Fourier-domain mode-locked laser absorption spectroscopy. Optics Express, no. 15, pp. 15115 - 15128, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Kranendonk_a,
      Title                    = {{High speed engine gas thermometry by Fourier-domain mode-locked laser absorption spectroscopy}},
      Author                   = {Kranendonk, Laura A and An, Xinliang and Caswell, Andrew W and Herold, Randy E and Sanders, Scott T and Huber, Robert and Fujimoto, James G and Okura, Yasuhiro and Urata, Yasuhiro},
      Journal                  = {Optics Express},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {23},
      Pages                    = {15115 -- 15128 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.015115},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-23-15115}
    }
  • Srinivasan, V J and Huber, R and Gorczynska, I and Fujimoto, J G and Jiang, J Y and Reisen, P and Cable, A E: High-speed, high-resolution optical coherence tomography retinal imaging with a frequency-swept laser at 850 nm. Optics Letters, no. 32, pp. 361 - 363, OSA, 2007
    BibTeX Link
    @Article{HU_2007_Srinivasan_a,
      Title                    = {{High-speed, high-resolution optical coherence tomography retinal imaging with a frequency-swept laser at 850 nm}},
      Author                   = {Srinivasan, V J and Huber, R and Gorczynska, I and Fujimoto, J G and Jiang, J Y and Reisen, P and Cable, A E},
      Journal                  = {Optics Letters},
      Year                     = {2007},
      Number                   = {4},
      Pages                    = {361 -- 363 },
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.000361},
      ISSN                     = {0146-9592},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-4-361}
    }
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Optical coherence tomography phase microscopy using buffered fourier domain mode locked (FDML) lasers at up to 370,000 lines per second. in Conference on Lasers and Electro-Optics, 2007, CLEO 2007, 2007
    BibTeX Link
    @InProceedings{HU_2007_Adler_e,
      Title                    = {{Optical coherence tomography phase microscopy using buffered fourier domain mode locked (FDML) lasers at up to 370,000 lines per second}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics, 2007, CLEO 2007},
      Year                     = {2007},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=CLEO-2007-CFL1}
    }
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers. Optics Letters, no. 32, pp. 626 - 628, 2007
    BibTeX Link
    @Article{HU_2007_Adler_c,
      Title                    = {{Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Letters},
      Year                     = {2007},
      Number                   = {6},
      Pages                    = {626 -- 628},
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.000626},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-6-626}
    }
    
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Phase-sensitive optical coherence tomography using buffered Fourier domain mode-locked lasers at up to 370,000 scans per second. in Biomedical Optics (BiOS) 2007, pp. 64291L-64291L-9, International Society for Optics and Photonics, feb,, 2007
    BibTeX Link
    @InProceedings{HU_2007_Adler_d,
      Title                    = {{Phase-sensitive optical coherence tomography using buffered Fourier domain mode-locked lasers at up to 370,000 scans per second}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Booktitle                = {Biomedical Optics (BiOS) 2007},
      Year                     = {2007},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {64291L--64291L--9},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.704128},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1334093}
    }
  • Huang, Shu-Wei and Aguirre, Aaron D and Huber, Robert A and Adler, Desmond C and Fujimoto, James G: Swept source optical coherence microscopy using a Fourier domain mode-locked laser. Optics Express, no. 15, pp. 6210 - 6217, 2007
    BibTeX Link
    @Article{HU_2007_Huang_a,
      Title                    = {{Swept source optical coherence microscopy using a Fourier domain mode-locked laser}},
      Author                   = {Huang, Shu-Wei and Aguirre, Aaron D and Huber, Robert A and Adler, Desmond C and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2007},
      Number                   = {10},
      Pages                    = {6210 -- 6217 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.006210},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-10-6210}
    }
  • Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G: Three-dimensional endomicroscopy using optical coherence tomography. Nature Photonics, no. 1, pp. 709-716, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Adler_a,
      Title                    = {{Three-dimensional endomicroscopy using optical coherence tomography}},
      Author                   = {Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G},
      Journal                  = {Nature Photonics},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {12},
      Pages                    = {709--716},
      Volume                   = {1},
    
      Doi                      = {10.1038/nphoton.2007.228},
      ISSN                     = {1749-4885},
      Url                      = {http://www.nature.com/doifinder/10.1038/nphoton.2007.228}
    }
  • Jenkins, M W and Adler, D C and Gargesha, M and Huber, R and Rothenberg, F and Belding, J and Watanabe, M and Wilson, D L and Fujimoto, J G and Rollins, A M: Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser. Optics Express, no. 15, pp. 6251 - 6267, 2007
    BibTeX Link
    @Article{HU_2007_Jenkins_a,
      Title                    = {{Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser}},
      Author                   = {Jenkins, M W and Adler, D C and Gargesha, M and Huber, R and Rothenberg, F and Belding, J and Watanabe, M and Wilson, D L and Fujimoto, J G and Rollins, A M},
      Journal                  = {Optics Express},
      Year                     = {2007},
      Number                   = {10},
      Pages                    = {6251 -- 6267 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.006251},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-10-6251}
    }
  • Kranendonk, Laura A and Huber, Robert and Fujimoto, James G and Sanders, Scott T: Wavelength-agile H2O absorption spectrometer for thermometry of general combustion gases. Proceedings of the Combustion Institute, no. 31, pp. 783-790, jan,, 2007
    BibTeX Link
    @Article{HU_2007_Kranendonk_b,
      Title                    = {{Wavelength-agile H2O absorption spectrometer for thermometry of general combustion gases}},
      Author                   = {Kranendonk, Laura A and Huber, Robert and Fujimoto, James G and Sanders, Scott T},
      Journal                  = {Proceedings of the Combustion Institute},
      Year                     = {2007},
    
      Month                    = jan,
      Number                   = {1},
      Pages                    = {783--790},
      Volume                   = {31},
    
      Doi                      = {10.1016/j.proci.2006.08.003},
      ISSN                     = {15407489},
      Url                      = {http://linkinghub.elsevier.com/retrieve/pii/S1540748906002665}
    }

2006

  • Sandeau, Julien and Caillibotte, Georges and Kandulla, Jochen and Birngruber, Reginald and Apiou-Sbirlea, Gabriela: Modeling of conductive and convective heat transfers in retinal laser treatments. no. 6138, pp. 61381A-61381A-9,
    BibTeX Link
    @inproceedings{Saneau2006,
       author = {Sandeau, Julien and Caillibotte, Georges and Kandulla, Jochen and Birngruber, Reginald and Apiou-Sbirlea, Gabriela},
       title = {Modeling of conductive and convective heat transfers in retinal laser treatments},
       volume = {6138},
       pages = {61381A-61381A-9},
       note = {10.1117/12.673494},
       abstract = {Tumor thermo treatment such as photodynamic therapy (PDT) or transpupillary thermotherapy (TTT) deal with long term and large laser spot exposures. The induced temperature increase is not exactly known [1]. Under these conditions convective heat transfers due to the blood flow in the choroid and the choriocapillaris must be considered in addition to the usually calculated heat conduction. From an existing analytical model defining a unique convective term for the whole fundus irradiated with Gaussian irradiance distribution lasers [2], we developed a numerical one allowing a precise modelling of convection and calculating heating evolution and temperature profiles of the fundus of the eye. The aim of this study is to present the modelling and several comparisons between experimental results [3] and numerical ones concerning the convective heat transfers inside the fundus of the eye.},
       url = {http://dx.doi.org/10.1117/12.673494},
       type = {Conference Proceedings}
    }
    
  • Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Hüttmann, G. and Birngruber, R. and Laqua, H.: Filtring Bleb Evaluation with Slit-Lamp-Adapted 1310-nm Optical Coherence Tomography. Curr Eye Res, no. 31, pp. 909-915, 2006
    BibTeX
    @article{Müller2006,
       author = {Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Hüttmann, G. and Birngruber, R. and Laqua, H.},
       title = {Filtring Bleb Evaluation with Slit-Lamp-Adapted 1310-nm Optical Coherence Tomography},
       journal = {Curr Eye Res},
       volume = {31},
       number = {11},
       pages = {909-915},
       year = {2006},
       type = {Journal Article}
    }
    
  • Elsner, H and Pörksen, E and Klatt, C and Bunse, A and Theisen-Kunde, D and Brinkmann, R and Birngruber, R and Laqua, H and Roider, J: Selective Retina Therapy (SRT) in patients with central serous chorioretinopathy (CSC). Graefes Arch Ophthalmol, in print., no. on-line: DOI 10.1007/s00417-006-0368-5, 2006
    BibTeX
    @article{Elsner2006,
       author = {Elsner, H and Pörksen, E and Klatt, C and Bunse, A and Theisen-Kunde, D and Brinkmann, R and Birngruber, R and Laqua, H and Roider, J},
       title = {Selective Retina Therapy (SRT) in patients with central serous chorioretinopathy (CSC)},
       journal = {Graefes Arch Ophthalmol, in print.},
       volume = {on-line: DOI 10.1007/s00417-006-0368-5},
       year = {2006},
       type = {Journal Article}
    }
    
  • Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Huttmann, G. and Birngruber, R. and Laqua, H.: Filtering bleb evaluation with slit-lamp-adapted 1310-nm optical coherence tomography. Current Eye Research, no. 31, pp. 909-915, 2006
    BibTeX Link
    @article{Müller2006,
       author = {Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Huttmann, G. and Birngruber, R. and Laqua, H.},
       title = {Filtering bleb evaluation with slit-lamp-adapted 1310-nm optical coherence tomography},
       journal = {Current Eye Research},
       volume = {31},
       number = {11},
       pages = {909-915},
       note = {107AB
    Times Cited:24
    Cited References Count:27},
       abstract = {Purpose: Investigation of slit-lamp-adapted 1310-nm optical coherence tomography (OCT) as an in vivo imaging device in the postoperative course of glaucoma surgery. Methods: Postoperative images of filtering blebs and deep sclerectomies and their healing processes were qualitatively evaluated with a slit-lamp-adapted anterior segment OCT (AS-OCT; Heidelberg Engineering, Heidelberg, Germany) in 28 patients. Ophthalmologic examinations included slit-lamp examination, applanation tonometry, and slit-lamp photography. The OCT scans were qualitatively correlated with the morphologic and functional outcome of the filtering bleb. Results: 1310-nm OCT was able to demonstrate the internal structure and the dimensions of filtering blebs, as well as the scleral flap and the deep sclerectomy location including Descemet membrane. Functioning filtering blebs showed a low OCT signal, small fluid-filled cysts, superficial microcystic layer, and a slack internal texture. High internal reflectivity indicated an earlier scarring of the filtering bleb. Nonfunctioning filtering blebs delivered a high OCT signal, no or few cysts, and a dense internal texture. These different OCT patterns correlated with the clinical outcome. Conclusions: Slit-lamp-adapted 1310-nm OCT allowed the noncontact observation and documentation of the postoperative healing course of filtering blebs after glaucoma surgery. Internal structures of the filtering bleb and deep sclerectomies could be visualized. Functioning and dysfunctioning filtering blebs delivered different OCT pattern and correlated with the clinical outcome. This could be a new way to assess the postoperative healing process with the possibility of earlier intervention in cases of impending scarring.},
       keywords = {deep sclerectomy
    filtering bleb
    oct
    scarring
    anterior segment
    mitomycin-c
    follow-up
    trabeculectomy
    surgery
    cornea
    eye},
       ISSN = {0271-3683},
       DOI = {Doi 10.1080/02713680600910528},
       url = {<Go to ISI>://WOS:000242142000003},
       year = {2006},
       type = {Journal Article}
    }
    
  • Klatt, C. and Elsner, H. and Porksen, E. and Brinkmann, R. and Bunse, A. and Birngruber, R. and Roider, J.: Selective retina therapy in central serous chorioretinopathy with detachment of the pigmentary epithelium. Ophthalmologe, no. 103, pp. 850-5, 2006
    BibTeX Link
    @article{Klatt2006,
       author = {Klatt, C. and Elsner, H. and Porksen, E. and Brinkmann, R. and Bunse, A. and Birngruber, R. and Roider, J.},
       title = {Selective retina therapy in central serous chorioretinopathy with detachment of the pigmentary epithelium},
       journal = {Ophthalmologe},
       volume = {103},
       number = {10},
       pages = {850-5},
       note = {0941-293X (Print)
    Case Reports
    English Abstract
    Journal Article},
       abstract = {BACKGROUND: Selective Retina Therapy (SRT) is a new and innovative laser treatment modality that selectively treats the retinal pigmentary epithelium while sparing the photoreceptors. This therapeutic concept appears to be particularly suitable for treating patients with acute or chronic central serous chorioretinopathy (CSC). We present preliminary results obtained in five patients who had CSC associated with pigmentary epithelium detachment (PED) and serous subretinal fluid (SRF) and who were treated with SRT. METHODS: This case series was made up of five male patients (mean age 47 years) with chronic CSC and SRF resulting from PED. Examinations performed before and at 1 month and 3 months after the treatment were: BCVA, FLA, OCT (Zeiss OCT III). For SRT, confluent treatment of the PED (area of leakage) was carried out using a pulsed frequency-doubled, Q-switched Nd-YLF prototype laser (lambda=527 nm, t= 1.7 s, 100 Hz, energy = 150-250 J). RESULTS: Best corrected visual acuity at baseline was 0.53, while after 4 weeks it was 0.56 and after 12 weeks, 0.5. At baseline leakage was seen at the PED on fluorescein angiography in all patients. After 4 weeks leakage activity was no longer noted on angiography in 4 of 5 patients. OCT at baseline showed SRF at the edge of the PED in all patients, but in 4 of the 5 patients this was no longer detectable after 4 weeks. CONCLUSION: SRT is a safe and effective treatment for patients with CSC in which PED has caused SRF. Not a single case of rip syndrome was observed in this study, even though the PED was treated confluently. Since SRT spares the photoreceptors it is particularly suitable for the treatment of CSC, especially when the origin of leakage is located close to the fovea. The results indicate that SRT leads to reconstruction of the outer blood-retina barrier.},
       keywords = {Adult
    Chorioretinitis/complications/*surgery
    Humans
    Laser Surgery/*methods
    Lasers/*therapeutic use
    Male
    Middle Aged
    Ophthalmologic Surgical Procedures/*methods
    Preoperative Care/methods
    Retinal Detachment/etiology/*surgery
    Treatment Outcome},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16937094},
       year = {2006},
       type = {Journal Article}
    }
    
  • Kandulla, J. and Elsner, H. and Birngruber, R. and Brinkmann, R.: Noninvasive optoacoustic online retinal temperature determination during continuous-wave laser irradiation. Journal of Biomedical Optics, no. 11, 2006
    BibTeX Link
    @article{Kandulla2006,
       author = {Kandulla, J. and Elsner, H. and Birngruber, R. and Brinkmann, R.},
       title = {Noninvasive optoacoustic online retinal temperature determination during continuous-wave laser irradiation},
       journal = {Journal of Biomedical Optics},
       volume = {11},
       number = {4},
       note = {093IM
    Times Cited:30
    Cited References Count:40},
       abstract = {The therapeutic effect of most retinal laser treatments is initiated by a transient temperature increase. Although crucial to the effectiveness of the treatment, the temperature course is not exactly known due to individually different tissue properties. We develop an optoacoustic method to determine the retinal temperature increase in real time during continuous-wave (cw) laser irradiation, and perform temperature calculations to interpret the results exemplary for transpupillary thermotherapy (TTT). Porcine globes ex vivo and rabbit eyes in vivo are irradiated with a diode laser (lambda=810 nm, P <= 3 W, phi = 2 mm) for 60 s. Simultaneously, pulses from a N-2-laser pumped dye laser (lambda= 500 nm, tau= 3.5 ns, E approximate to 5 mu J) are applied on the retina. Following its absorption, an ultrasonic pressure wave is emitted, which is detected by a transducer embedded in a contact lens. Using the previously measured temperature-dependent Gruneisen coefficient of chorioretinal tissue, a temperature raise in porcine eyes of 5.8 degrees C/ (W/cm(2)) after 60 s is observed and confirmed by simultaneous measurements with an inserted thermocouple. In a rabbit, we find 1.4 degrees C/(W/cm(2)) with, and 2.2 degrees C/(W/cm(2)) without perfusion at the same location. Coagulation of the rabbit's retina occurs at Delta T = 21 degrees C after 40 s. In conclusion, this optoacoustic method seems feasible for an in vivo real-time determination of temperature, opening the possibility for feedback control retinal laser treatments. (c) 2006 Society of Photo-Optical Instrumentation Engineers.},
       keywords = {temperature
    optoacoustics
    transpupillary thermotherapy
    ultrasonic transducer
    laser
    heat diffusion calculations
    retinal laser treatment
    subfoveal choroidal neovascularization
    transpupillary thermotherapy
    macular degeneration
    ocular media
    blood flow
    eye
    fundus
    light
    photocoagulation
    transmission},
       ISSN = {1083-3668},
       DOI = {Artn 041111
    Doi 10.1117/1.2236301},
       url = {<Go to ISI>://WOS:000241162000016},
       year = {2006},
       type = {Journal Article}
    }
    
  • Hoerauf, H. and Brix, A. and Winkler, J. and Droege, G. and Winter, C. and Birngruber, R. and Laqua, H. and Vogel, A.: Photoablation of inner limiting membrane and inner retinal layers using the erbium : YAG-laser: An in vitro study. Lasers in Surgery and Medicine, no. 38, pp. 52-61, 2006
    BibTeX Link
    @article{Hoerauf2006,
       author = {Hoerauf, H. and Brix, A. and Winkler, J. and Droege, G. and Winter, C. and Birngruber, R. and Laqua, H. and Vogel, A.},
       title = {Photoablation of inner limiting membrane and inner retinal layers using the erbium : YAG-laser: An in vitro study},
       journal = {Lasers in Surgery and Medicine},
       volume = {38},
       number = {1},
       pages = {52-61},
       note = {009YN
    Times Cited:4
    Cited References Count:51},
       abstract = {Background and Objectives: To explore the potential of Er:YAG-laser irradiation for precise and tractionless retinal tissue and inner limiting membrane ablation.
    Materials and Methods: We used free-running Er:YAG-laser irradiation (lambda = 2.94 mu m) transmitted either through a 10 em long low-OH-quartz fiber or a 2 m long sapphire fiber that produced a more homogenous light distribution at the fiber tip. Retinal ablation in porcine retinal explants was performed under air or perfluorodecaline (PFD). Ablation depth was evaluated by optical coherence tomography (OCT) and from histologic sections.
    Results: A radiant exposure of 5.0 J/cm(2) delivered through a low-OH-quartz fiber and PFD caused a complete transsection of the neurosensory retina. Radiant exposures between 3.5 and 2.0 J/cm(2) resulted in marked variations of ablation depth and adjacent thermal damage. By contrast, laser pulses of 4.0 and 3.0 J/cm(2) transmitted through the sapphire fiber produced more homogenous defect patterns and less thermal damage. Close to the ablation threshold, with 1.0-2.0 J/cm(2), ablation was limited to a 10-20 mu m thin layer of the neural retina.
    Conclusions: We achieved in vitro ablation of inner retinal layers, but could not produce selective and reproducible ILM removal.},
       keywords = {macular surgery
    optical coherence tomography
    perfluorocarbon liquid
    retina
    retinal explant
    optical coherence tomography
    macular hole surgery
    experimental vitreous membranes
    er-yag
    vitreoretinal surgery
    clinical-experience
    indocyanine green
    excimer-laser
    pig eyes
    ablation},
       ISSN = {0196-8092},
       DOI = {Doi 10.1002/Lsm.20269},
       url = {<Go to ISI>://WOS:000235149600007},
       year = {2006},
       type = {Journal Article}
    }
    
  • Framme, C. and Flucke, B. and Birngruber, R.: Comparison of reduced and standard light application in photodynamic therapy of the eye in two rabbit models. Graefes Arch Clin Exp Ophthalmol, no. 244, pp. 773-81, 2006
    BibTeX Link
    @article{Famme2006,
       author = {Framme, C. and Flucke, B. and Birngruber, R.},
       title = {Comparison of reduced and standard light application in photodynamic therapy of the eye in two rabbit models},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {244},
       number = {7},
       pages = {773-81},
       note = {0721-832X (Print)
    Comparative Study
    Journal Article},
       abstract = {BACKGROUND: Current PDT treatment for age-related macular degeneration uses a standard radiant exposure of 50 J/cm(2) at an irradiance of 600 mW/cm(2). However; there is a general problem with the unusually high irradiance; in fact, the rate of photochemical production of singlet oxygen may be limited by insufficiently oxygenized neovascular tissue. It was the aim of this study to evaluate the efficacy of verteporfin (Visudyne) photoactivation to induce thrombosis of choriocapillaries and in experimentally induced corneal neovascularizations in rabbits by varying irradiance and retinal radiant exposure. MATERIALS AND METHODS: The light-dose threshold to induce micro-thrombosis in the choriocapillaries (seven eyes) and in corneal neovascularizations (eight eyes) of Chinchilla-Bastard rabbits using different retinal irradiances (100 and 600 mW/cm(2)) at different radiant exposures (20, 10, 5, 2.5, 1.25, 0.62, and 0.3 J/cm(2)) was evaluated. Induction of neovascularizations was performed 7 days prior to PDT treatment using intracorneal silk sutures. A dose of 2 mg/kg verteporfin was intravenously infused 10 min before standard PDT. The criterion for vascular thrombosis was vessel closure as determined by fluorescein angiography 1 h and 1 day post exposure. RESULTS: Experiments on the choroid revealed vessel closure 1 h after irradiation at ED(50) = 10.8 J/cm(2) (both 600 and 100 mW/cm(2)) and after 24 h at ED(50) = 2.4 J/cm(2) (600 mW/cm(2)) versus 1.8 J/cm(2) (100 mW/cm(2)). Vessel closure was enhanced at irradiation with 100 mW/cm(2). Regarding corneal neovascularizations, vessel thrombosis was observable by dark appearance of irradiated clotted neovascular tissue and angiographically by a lack of leakage at ED(50) thresholds of 0.62 J/cm(2) (1 h) and 0.41 J/cm(2) (1 day) for 100 mW/cm(2) and of 0.99 J/cm(2) (1 h), and 0.67 J/cm(2) (1 day) for 600 mW/cm(2). Thus in both experiments thresholds for vessel closure were reduced by a factor of 1.5 for the lower intensity. Histology revealed more selective vessel occlusion without RPE and photoreceptor damage for 100 mW/cm(2) rather than 600 mW/cm(2) intensity at threshold irradiation. CONCLUSION: Low-intensity PDT with verteporfin for neovascular tissue seems to be more effective than regular high-intensity PDT. Future preclinical trials should address the issue of proper dosimetry for effective PDT in age-related macular degeneration.},
       keywords = {Animals
    Choroidal Neovascularization/*drug therapy
    Corneal Neovascularization/*drug therapy
    *Disease Models, Animal
    Fluorescein Angiography
    Photochemotherapy/*methods
    Photosensitizing Agents/*therapeutic use
    Porphyrins/*therapeutic use
    Rabbits
    Radiation Dosage},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16440207},
       year = {2006},
       type = {Journal Article}
    }
    
  • Framme, C. and Sachs, H. G. and Flucke, B. and Theisen-Kunde, D. and Birngruber, R.: Evaluation of the new photosensitizer Tookad (WST09) for photodynamic vessel occlusion of the choroidal tissue in rabbits. Invest Ophthalmol Vis Sci, no. 47, pp. 5437-46, 2006
    BibTeX Link
    @article{Framme2006,
       author = {Framme, C. and Sachs, H. G. and Flucke, B. and Theisen-Kunde, D. and Birngruber, R.},
       title = {Evaluation of the new photosensitizer Tookad (WST09) for photodynamic vessel occlusion of the choroidal tissue in rabbits},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {47},
       number = {12},
       pages = {5437-46},
       note = {0146-0404 (Print)
    Journal Article
    Research Support, Non-U.S. Gov't},
       abstract = {PURPOSE: To determine the efficacy of Tookad (WST09; Negma-Lerads, Magny-Les-Hameaux, France) photodynamic therapy (T-PDT) by evaluating the angiographic and histologic closure of choroidal vessels at different radiance exposures, drug dosages, and intervals between photosensitizer injection and laser application in a rabbit model. METHODS: Chinchilla Bastard rabbits were injected intravenously with three different dye concentrations (2.5, 5, and 10 mg/kg) before application of light. In every group T-PDT was performed at four different times after injection: 5, 15, 30, and 60 minutes with different radiance exposures ranging from 200 to 3 J/cm2. Fundus photographs and fluorescein angiograms were obtained 90 minutes after injection. Follow-up angiographies were performed at days 1, 3, 7, and 14 after initial treatment. Histology was performed in selected cases immediately after treatment and on days 1, 3, and 7. RESULTS: Immediately after irradiation, most of the visible lesions were angiographically hyperfluorescent due to damaged vessel endothelium and associated RPE damage. Lesions from high-radiance exposures revealed immediate hypofluorescence, indicating vessel closure. Hypofluorescent lesions appeared mainly during day 1 (all lesions angiographically visible, some hypofluorescent) to day 3 (all lesions hypofluorescent) after treatment. At day 7, ophthalmoscopically visible hyperpigmentation took place in all lesions. ED50 thresholds for angiographic hypofluorescence determined at day 3 after treatment with 2.5 mg/kg were 18.8 J/cm2 (5 minutes), 62.0 J/cm2 (15 minutes), and >100 J/cm2 (30 minutes); with 5 mg/kg, 8.4 J/cm2 (5 minutes), 22.8 J/cm2 (15 minutes), 54.5 J/cm2 (30 minutes), and >100 J/cm2 (60 minutes); and with 10 mg/kg, 11.7 J/cm2 (30 minutes) and 54.1 J/cm2 (60 minutes). Histology of the angiographically hypofluorescent lesions revealed vessel thrombosis in all groups 1 hour after PDT up to 7 days after treatment. Sparing of photoreceptors indicated selectivity of T-PDT; however, slight damage was partly observable. After 7 days, localized proliferation of the RPE cells was noted and was enhanced 14 days after treatment. CONCLUSIONS: T-PDT has the potential to achieve selective choroidal vessel occlusion with proper parameter selection, such as (1) 2.5 mg/kg, 5 minutes, 100 J/cm2; (2) 5 mg/kg, 5 minutes, 25 J/cm2; or (3) 5 mg/kg, 15 minutes, 50 J/cm2; however, slight damage to the photoreceptors cannot be ruled out. RPE proliferation indicates primary RPE damage due to PDT, also described with the use of all other photosensitizers.},
       keywords = {Animals
    Bacteriochlorophylls/*administration & dosage
    Choroid/*blood supply/drug effects/pathology
    Choroidal Neovascularization/diagnosis/*drug therapy
    Drug Evaluation, Preclinical
    Fluorescein Angiography
    *Photochemotherapy
    Photosensitizing Agents/*administration & dosage
    Rabbits},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17122134},
       year = {2006},
       type = {Journal Article}
    }
    
  • Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.: Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.. Proc SPIE, no. 6078, pp. 495-502, 2006
    BibTeX
    @article{Giese2006,
       author = {Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.},
       title = {Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.},
       journal = {Proc SPIE},
       volume = {6078},
       pages = {495-502},
       year = {2006},
       type = {Journal Article}
    }
    
  • Ahlers, C. and Michels, S. and Beckendorf, A. and Birngruber, R. and Schmidt-Erfurth, U.: Three-dimensional imaging of pigment epithelial detachment in age-related macular degeneration using optical coherence tomography, retinal thickness analysis and topographic angiography. Graefes Archive for Clinical and Experimental Ophthalmology, no. 244, pp. 1233-1239, 2006
    BibTeX Link
    @article{Ahlers2006,
    
       author = {Ahlers, C. and Michels, S. and Beckendorf, A. and Birngruber, R. and Schmidt-Erfurth, U.},
       title = {Three-dimensional imaging of pigment epithelial detachment in age-related macular degeneration using optical coherence tomography, retinal thickness analysis and topographic angiography},
       journal = {Graefes Archive for Clinical and Experimental Ophthalmology},
       volume = {244},
       number = {10},
       pages = {1233-1239},
       note = {087FX
    Times Cited:16
    Cited References Count:19},
       abstract = {Introduction: New diagnostic tools such as the retinal thickness analyzer (RTA), optical coherence tomography (OCT), and topographic angiography (TAG) were introduced into clinical ophthalmology during the last years giving the examiner new insights into anatomical and functional aspects of macular disease. In this study, advantages and disadvantages of the new imaging methods have been evaluated in patients with serous (sPED) and fibrovascular pigment epithelial detachments (fPED) secondary to age-related macular degeneration (AMD). Methods: TAG, using fluorescein angiography (FA), provides a three-dimensional profile of the fluorescein pattern based on the analysis of a set of 32 confocal images over a depth of 4 mm. RTA and OCT provide cross-sectional images of the neurosensory retina and the retinal pigment epithelium-choriocapillary complex as well as retinal thickness data encoded in a false color map. We compared and evaluated these modalities in 15 patients with fPED and 15 patients with sPED secondary to AMD. Results: In patients with classic fPED, TAG detected neovascular structures and delineated their configuration. In sPEDs, pooling of extravascular fluid was detected in a dome-shaped configuration. OCT provided detailed information on the neurosensory retina's structures but failed to detect the neovascular membrane in fPED. Mapping the retinal thickness, RTA and OCT both failed to detect the PED and showed typical algorithm error-based patterns. Conclusions: TAG OCT and RTA are useful imaging modalities in the evaluation of AMD cases. TAG visualizes the vascular configuration, dynamic perfusion, and leakage changes. OCT and RTA are able to complementarily document intra-, subretinal, and sub-RPE fluid accumulation secondary to CNV. However, OCT seems to be more efficient in imaging AMD-related pathologies than RTA, as this modality is often compromised by intra- or subretinal structural abnormalities. Nevertheless, all modalities may provide further valuable insight into AMD pathogenesis, enhance diagnostic quality, and improve the assessment of therapeutic effects.},
       keywords = {choroidal neovascularization
    pigment epithelial detachment (ped)
    topographic angiography
    retinal thickness analyzer
    age-related macular degeneration (amd)
    optical coherence tomography
    imaging
    choroidal neovascularization
    verteporfin therapy
    disease
    repeatability
    eyes
    oct
    tap},
       ISSN = {0721-832X},
       DOI = {DOI 10.1007/s00417-006-0418-z},
       url = {<Go to ISI>://WOS:000240729400003},
       year = {2006},
       type = {Journal Article}
    }
    
  • Roider, J.: Selektive Retina Therapie – SRT. Der Ophthalmologe, no. 103, pp. 837-838, 2006
    BibTeX
    @article{Roider,
       author = {Roider, J.},
       title = {Selektive Retina Therapie – SRT},
       journal = {Der Ophthalmologe},
       volume = {103},
       number = {10},
       pages = {837-838},
       year = {2006}
    }
  • Brujan, EA. and Vogel, A .: Stress wave emission and cavitation bubble dynamics by nanosecond optical breakdown in a tissue phantom.. J Fluid Mech, no. 558, pp. 281-308, 2006
    BibTeX
    @article{Brujan,
       author = {Brujan, EA. and Vogel, A .},
       title = {Stress wave emission and cavitation bubble dynamics by nanosecond optical breakdown in a tissue phantom.},
       journal = { J Fluid Mech},
       volume = {558},
       pages = {281-308},
       year = {2006}
    }
  • Daniltchenko, D.: Anwendung der optischen Kohärenztomographie (OCT) bei der Darstellung von Urothelerkrankungen der Harnblase. Der Radiologe, no. 46, pp. 584, 2006
    BibTeX
    @article{Daniltchenko,
       author = {Daniltchenko, D.},
       title = {Anwendung der optischen Kohärenztomographie (OCT) bei der Darstellung von Urothelerkrankungen der Harnblase},
       journal = {Der Radiologe},
       volume = {46},
       number = {7},
       pages = {584},
       year = {2006}
    }
  • Brinkmann, Ralf and Birngruber, Reginald: Selektive Retina-Therapie (SRT). Zeitschrift für Medizinische Physik, no. 17, pp. 6-22, 2006
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, Ralf and Birngruber, Reginald},
       title = {Selektive Retina-Therapie (SRT)},
       journal = {Zeitschrift für Medizinische Physik},
       volume = {17},
       number = {1},
       pages = {6-22},
       abstract = {Zusammenfassung Die am Medizinischen Laserzentrum Lübeck entwickelte selektive Retina-Therapie (SRT) wird zur Zeit als neue, schonende Laser-Behandlungsmethode für verschiedene Erkrankungen des Augenhintergrunds evaluiert, deren Ursachen einer Degradation des Retinalen Pigmentepithels (RPE) zugeschrieben werden. Mit der SRT lässt sich selektiv das RPE behandeln, ohne die angrenzende neurosensorische Netzhaut mit den Photorezeptoren und die unter dem RPE liegende Aderhaut (Choroidea) zu beeinträchtigen. Die Therapie führt idealerweise zu einer Regeneration des RPEs und einem gesteigerten Metabolismus am chorio-retinalen Übergang. Im Gegensatz zur etablierten Laserphotokoagulation, bei der die Netzhaut in und um die bestrahlten Areale komplett verödet wird, bleibt bei der SRT die Sehfähigkeit der Patienten in den bestrahlten Arealen erhalten. Der Artikel gibt einen Überblick über die Idee und die physikalischen Mechanismen selektiver RPE-Behandlung, die online Dosimetrie der optisch nicht sichtbaren Effekte und fasst die ersten klinischen Ergebnisse zusammen. Selective Retina Therapy (SRT) is a new and very gentle laser method developed at the Medical Laser Center Lübeck. It is currently investigated clinically in order to treat retinal disorders associated with a decreased function of the retinal pigment epithelium (RPE). SRT is designed to selectively effect the RPE while sparing the neural retina and the photoreceptors as well as the chorioidea. Aim of the therapy is the rejuvenation of the RPE in the treated areas, which should ideally lead to a long term metabolic increase at the chorio-retinal junction. In contrast to conventional laser photocoagulation, which is associated with a complete thermal necrosis of the treated site, SRT completely retains full vision. This paper reviews the methods and mechanisms behind selective RPE effects and reports the first clinical results. An online dosimetry technique to visualize the ophthalmoscopically invisible effects is introduced.},
       keywords = {Selektive Zelleffekte, Optoakustik, Mikroblasen, Online-Dosimetrie, RPE, ?s-Laserpulse, Makulaödeme, RCS
    Selective cellular effects, optoacoustics, online dosimetry, RPE, ?s-laser pulses, macula oedema, RCS},
       year = {2006}
    }
  • Diddens, H. and Arp, N. and Eisenbeiß, W.: Photodynamische Therapie zur Behandlung lokaler Wunden. GMS Krankenhaushyg Interdiszip, no. 1, pp. Doc 19, 2006
    BibTeX
    @article{Diddens,
       author = {Diddens, H. and Arp, N. and Eisenbeiß, W.},
       title = {Photodynamische Therapie zur Behandlung lokaler Wunden},
       journal = {GMS Krankenhaushyg Interdiszip},
       volume = {1},
       number = {1},
       pages = {Doc 19},
       year = {2006}
    }
  • Elsner, H. and Klatt, C. and Liew, S. H. and Porksen, E. and Bunse, A. and Rudolf, M. and Brinkmann, R. and Hamilton, R. P. and Birngruber, R. and Laqua, H. and Roider, J.: Selektive Retina Therapiey (SRT) bei Patienten mit diabetischer Makulopathie. Ophthalmologe, no. 103, pp. 856-860, 2006
    BibTeX
    @article{Elsner2006,
       author = {Elsner, H. and Klatt, C. and Liew, S. H. and Porksen, E. and Bunse, A. and Rudolf, M. and Brinkmann, R. and Hamilton, R. P. and Birngruber, R. and Laqua, H. and Roider, J.},
       title = {Selektive Retina Therapiey (SRT) bei Patienten mit diabetischer Makulopathie},
       journal = {Ophthalmologe},
       volume = {103},
       number = {10},
       pages = {856-860},
       note = {0941-293X (Print)
    Journal article},
       abstract = {Selective Retina Therapy (SRT) is a new laser treatment that selectively targets the retinal pigmen epithelium (RPE). In this study, we treated 39 patients presenting with nonischemic, focal and focal-diffuse diabetic maculopathy with SRT. In the main. the results indicate that SRT had stabilizing effects on visual acuity, angiographic leakage, lipid exudation, and foveal retinal thickness. SRT is safe and is especially useful for treating pathologies that are located close to the fovea, which cannot be treated with conventional argon laser photocoagulation.},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16937095},
       year = {2006},
       type = {Journal Article}
    }
    
  • Kandulla, J and Elsner, H and Sandeau, J and Birngruber, R and Brinkmann, R: Non-invasive optoacoustic temperature determination during retinal cw-laser treatments. in Proc SPIE, no. 6138, pp. 336-343,
    BibTeX
    @inproceedings{Kandulla2006,
       author = {Kandulla, J and Elsner, H and Sandeau, J and Birngruber, R and Brinkmann, R},
       title = {Non-invasive optoacoustic temperature determination during retinal cw-laser treatments},
       booktitle = {Proc SPIE},
       volume = {6138},
       pages = {336-343},
       type = {Conference Proceedings}
    }
    
  • Ueda, S and Hayashi, Y and Kohno, T and Miki, N and Hishida, E and Shiraki, K: A case of pachymeningitis with impaired ocular motility.. Japanese Journal of Clinical Ophthalmology, no. 60, pp. 553-557, 2006
    BibTeX
    @article{Hayashi,
       author = {Ueda, S and Hayashi, Y and Kohno, T and Miki, N and Hishida, E and Shiraki, K},
       title = {A case of pachymeningitis with impaired ocular motility.},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {60},
       number = {4},
       pages = {553-557},
       year = {2006}
    }
    
  • Yamamoto, M and Kohno, T and Murasawa, M and Ono, H and Ueda, S and Hayashi, Y and Ataka, S and Shiraki, K: Lipofuscin autofluorescence in a case of crystalline retinopathy.. Japanese Journal of Clinical Ophthalmology, no. 60, pp. 1039-1043, 2006
    BibTeX
    @article{Hayashi,
       author = {Yamamoto, M and Kohno, T and Murasawa, M and Ono, H and Ueda, S and Hayashi, Y and Ataka, S and Shiraki, K},
       title = {Lipofuscin autofluorescence in a case of crystalline retinopathy.},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {60},
       number = {6},
       pages = {1039-1043},
       year = {2006}
    }
    
  • Brinkmann, R. and Roider, J. and Birngruber, R.: Selective retina therapy (SRT): a review on methods, techniques, preclinical and first clinical results. Bull Soc Belge Ophtalmol, pp. 51-69, 2006
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R. and Roider, J. and Birngruber, R.},
       title = {Selective retina therapy (SRT): a review on methods, techniques, preclinical and first clinical results},
       journal = {Bull Soc Belge Ophtalmol},
       number = {302},
       pages = {51-69},
       note = {0081-0746 (Print)
    Journal Article
    Review},
       abstract = {Selective retina therapy (SRT) is a new laser procedure for retinal diseases that are thought to be associated with a degradation of the retinal pigment epithelium (RPE). The aim of the irradiation is to selectively damage the RPE without affecting the neural retina, the photoreceptors and the choroid. Goal of the treatment is to stimulate RPE cell migration and proliferation into the irradiated areas in order to improve the metabolism at the diseased retinal sites. In a pilot study more than 150 patients with soft drusen, retinopathia centralis serosa (RCS) and macular edema were treated. The first 3-center international trial targets diabetic macular edema and branch vein occlusion. In this review, selective RPE effects are motivated and two modalities to achieve selective RPE effects will be introduced: a pulsed and a continuous wave scanning mode. The mechanism behind selective RPE-effects will be discussed reviewing in vitro results and temperature calculations. So far clinical SRT is performed by applying trains of 30 laser pulses from a Nd:YLF-Laser (527 nm, 1.7 micros, 100 Hz) to the diseased fundus areas. In the range of 450-800 mJ/cm(2) per pulse, RPE-defects in patients were proved angiographically by fluorescein or ICG-leakage. The selectivity with respect to surrounding highly sensitive tissue and the safety range of the treatment will be reviewed. With the laser parameters used neither bleeding nor scotoma, proved by microperimetry, were observed thus demonstrating no adverse effects to the choroid and the photoreceptors, respectively. During and after irradiation, it shows that the irradiated locations are ophthalmoscopically invisible, since the effects are very limited and confined to the RPE, thus a dosimetry control is demanded. We report on a non-invasive opto-acoustic on-line technique to monitor successful RPE-irradiation and compare the data to those achieved with standard angiography one-hour post treatment.},
       keywords = {Fluorescein Angiography
    Humans
    Laser Coagulation/adverse effects/*methods
    Multicenter Studies
    Perimetry
    Pigment Epithelium of Eye/pathology/surgery
    Retinal Diseases/diagnosis/pathology/*surgery
    Scotoma/etiology/prevention & control},
       year = {2006}
    }
  • Vogel, A. and Apitz, I. and Freidank, S. and Dijkink, R.: Sensitive high-resolution white-light Schlieren technique with a large dynamic range for the investigation of ablation dynamics. Opt. Lett., no. 31, pp. 1812-1814, 2006
    BibTeX
    @article{Vogel2006,
       author = {Vogel, A. and Apitz, I. and Freidank, S. and Dijkink, R.},
       title = {Sensitive high-resolution white-light Schlieren technique with a large dynamic range for the investigation of ablation dynamics},
       journal = {Opt. Lett.},
       volume = {31},
       pages = {1812-1814},
       year = {2006}
    }
  • Tanaka, R and Hayashi, Y and Kohno, T and Yamazaki, K and Shiraki, K: Retinal phlebitis in a case of erythema induratum of Bazin. Japanese Journal of Clinical Ophthalmology, no. 60, pp. 1901-1904, 2006
    BibTeX
    @article{Hayashi,
       author = {Tanaka, R and Hayashi, Y and Kohno, T and Yamazaki, K and Shiraki, K},
       title = {Retinal phlebitis in a case of erythema induratum of Bazin},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {60},
       number = {12},
       pages = {1901-1904},
       abstract = {Purpose?To report a case of erythema of Bazin associated with retinal phlebitis. Case and Findings?A 59-year-old woman developed painful exanthema in both legs,followed by blurring and seeing flies in her left eye one month later. She was strongly positive to tuberculin test. Biopsy of erythema showed epithelioid granuloma with Langhans giant cells,leading to the diagnosis of Bazin disease. When seen 6 weeks after onset of blurring,her corrected visual acuity was 1.2 right and 1.0 left. Her left eye showed signs of retinal phlebitis. Peroral corticosteroid failed to improve skin and fundus lesions. Similar retinal phlebitis developed in the right eye 2 months later. Pulsed corticosteroid followed by tapering was followed by gradual improvement of skin and eye manifestations. Conclusion?Pulsed corticosteroid therapy may be effective for skin and eye manifestations of erythema of Bazin.},
       year = {2006}
    }
    
  • Tamaoki, S and Hayashi, Y and Sakaguchi, H and Tanaka, N and Ota, M and Yokoyama, T and Shiraki, K: Retinoblastoma in the patient with 13q-syndrome: a report of two cases.. Japanese Review of Clinical Ophthalmology, no. 100, pp. 587-590, 2006
    BibTeX
    @article{Hayashi,
       author = {Tamaoki, S and Hayashi, Y and Sakaguchi, H and Tanaka, N and Ota, M and Yokoyama, T and Shiraki, K},
       title = {Retinoblastoma in the patient with 13q-syndrome: a report of two cases.},
       journal = {Japanese Review of Clinical Ophthalmology},
       volume = {100},
       number = {8},
       pages = {587-590},
       year = {2006}
    }
    
  • Narumi, T and Hayashi, Y and Ataka, S and Tanaka, R and Murasawa, M and Kohno, T and Shiraki, K: Blunt ocular trauma in a patient with hemophilia-A. Japanese Journal of Clinical Ophthalmology, no. 60, pp. 1653-1656, 2006
    BibTeX
    @article{Hayashi,
       author = {Narumi, T and Hayashi, Y and Ataka, S and Tanaka, R and Murasawa, M and Kohno, T and Shiraki, K},
       title = {Blunt ocular trauma in a patient with hemophilia-A},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {60},
       number = {9},
       pages = {1653-1656},
       abstract = {Purpose?To report a patient with hemophilia-A who was treated by recombinant factor VIII concentrate for trauma-induced hyphema. Case and Findings?A 18-year-old male was hit by a flying softball on his left eye. He noted pain and blurring of left vision. When seen the next day,his visual acuity was hand motion left with intraocular pressure?IOP?of 37mmHg. The left cornea was edematous. The anterior chamber was filled with blood. He was diagnosed with hemophilia A with factor VIII level of 3?. His bleeding time was within normal limit. Irrigation of anterior was performed without incident after intravenous injection of recombinant factor VIII concentrate. Vitreous hemorrhage started to be absorbed 3 weeks after the trauma. Visual acuity improved to 0.02 5 weeks and to 1.5 9 weeks after trauma. Conclusion?Systemic recombinant factor VIII concentrate seemed to facilitate irrigation of anterior chamber and spontaneous absorption of vitreous hemorrhage in a patient with hemophilia-A.},
       year = {2006}
    }
    
  • Hishida, E and Hayashi, Y. and Narumi, T and Shiraki, K.: A case of Tolosa-Hunt syndrome with optic neuritis and impaired visual acuity. Japanese Journal of Clinical Ophthalmology, no. 60, pp. 943-946, 2006
    BibTeX
    @article{Hayashi2006,
       author = {Hishida, E and Hayashi, Y. and Narumi, T and Shiraki, K.},
       title = {A case of Tolosa-Hunt syndrome with optic neuritis and impaired visual acuity},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {60},
       number = {6},
       pages = {943-946},
       abstract = {Purpose?To report a case of Tolosa-Hunt syndrome with optic neuritis and impaired visual acuity. Case and Findings?A 31-year-old woman presented with retrobulbar pain in her left eye. Her corrected visual acuity was 1.2 right and 0.8 left. The left eye developed total ophthalmoplegia with visual acuity of 0.08 four days later. She was diagnosed with Tolosa-Hunt syndrome with optic neuritis. Pulsed corticosteroid therapy induced improved ocular motility with reduced pain. Visual acuity improved to 0.7. Discontinuation of corticosteroid was followed by severe recurrence. Left visual acuity was reduced to no light perception. Another course of pulsed corticosteroid therapy with peroral prednisolone induced improvement. There has been no recurrence until 9months later. Conclusion?Pulsed corticosteroid therapy with peroral prednisolone may be useful in preventing recurrence of optic neuritis in Tolosa-Hunt syndrome.},
       year = {2006}
    }
    
  • Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.: Femtosecond laser nanosurgery of biological cells and tissues. in 4th International Congress on Laser Advanced Materials Processing,
    BibTeX
    @inproceedings{Vogel2006,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Femtosecond laser nanosurgery of biological cells and tissues},
       booktitle = {4th International Congress on Laser Advanced Materials Processing}
    }
  • Brinkmann, R. and Roider, J. and Birngruber, R.: Selective retina therapy (SRT): a review on methods, techniques, preclinical and first clinical results. Bull Soc Belge Ophtalmol, pp. 51-69, 2006
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R. and Roider, J. and Birngruber, R.},
       title = {Selective retina therapy (SRT): a review on methods, techniques, preclinical and first clinical results},
       journal = {Bull Soc Belge Ophtalmol},
       number = {302},
       pages = {51-69},
       note = {0081-0746 (Print)
    Journal Article
    Review},
       abstract = {Selective retina therapy (SRT) is a new laser procedure for retinal diseases that are thought to be associated with a degradation of the retinal pigment epithelium (RPE). The aim of the irradiation is to selectively damage the RPE without affecting the neural retina, the photoreceptors and the choroid. Goal of the treatment is to stimulate RPE cell migration and proliferation into the irradiated areas in order to improve the metabolism at the diseased retinal sites. In a pilot study more than 150 patients with soft drusen, retinopathia centralis serosa (RCS) and macular edema were treated. The first 3-center international trial targets diabetic macular edema and branch vein occlusion. In this review, selective RPE effects are motivated and two modalities to achieve selective RPE effects will be introduced: a pulsed and a continuous wave scanning mode. The mechanism behind selective RPE-effects will be discussed reviewing in vitro results and temperature calculations. So far clinical SRT is performed by applying trains of 30 laser pulses from a Nd:YLF-Laser (527 nm, 1.7 micros, 100 Hz) to the diseased fundus areas. In the range of 450-800 mJ/cm(2) per pulse, RPE-defects in patients were proved angiographically by fluorescein or ICG-leakage. The selectivity with respect to surrounding highly sensitive tissue and the safety range of the treatment will be reviewed. With the laser parameters used neither bleeding nor scotoma, proved by microperimetry, were observed thus demonstrating no adverse effects to the choroid and the photoreceptors, respectively. During and after irradiation, it shows that the irradiated locations are ophthalmoscopically invisible, since the effects are very limited and confined to the RPE, thus a dosimetry control is demanded. We report on a non-invasive opto-acoustic on-line technique to monitor successful RPE-irradiation and compare the data to those achieved with standard angiography one-hour post treatment.},
       keywords = {Fluorescein Angiography
    Humans
    Laser Coagulation/adverse effects/*methods
    Multicenter Studies
    Perimetry
    Pigment Epithelium of Eye/pathology/surgery
    Retinal Diseases/diagnosis/pathology/*surgery
    Scotoma/etiology/prevention & control},
       year = {2006}
    }
  • Brinkmann, R and Schuele, G. and Neumann, J. and Framme, C. and Pörkensen, E. and Elsner, H. and Theisen-Kunde, D and Roider, J. and Birngruber, R.: Selektive Retina Therapie - Methodik, Technik und Online-Dosimetrie. Ophthalmologe, no. 103, pp. 839-849, 2006
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R and Schuele, G. and Neumann, J. and Framme, C. and Pörkensen, E. and Elsner, H. and Theisen-Kunde, D and Roider, J. and Birngruber, R.},
       title = {Selektive Retina Therapie - Methodik, Technik und Online-Dosimetrie},
       journal = {Ophthalmologe},
       volume = {103},
       pages = {839-849},
       year = {2006}
    }
  • Vogel, A. and Noack, J and Hüttmann, G. and Paltauf, G.: Femtosecond plasma-mediated nanosurgery of cells and tissues.. in Laser Ablation, pp. 217-262, Springer, Heidelberg, 2006
    BibTeX
    @inbook{Vogel2006,
       author = {Vogel, A. and Noack, J and Hüttmann, G. and Paltauf, G.},
       title = {Femtosecond plasma-mediated nanosurgery of cells and tissues.},
       booktitle = {Laser Ablation},
       editor = {), Phipps C (Hrsg.},
       publisher = { Springer, Heidelberg},
       pages = {217-262},
       year = {2006}
    }
  • Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.: Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.. Proc SPIE, no. 6078, pp. 495-502, 2006
    BibTeX
    @article{Giese,
       author = {Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.},
       title = {Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.},
       journal = {Proc SPIE},
       volume = {6078},
       pages = {495-502},
       year = {2006}
    }
  • Neumann, J. and Brinkmann, R: Cell disintegration by laser-induced transient microbubbles and its simultaneous monitoring by interferometry. Journal of Biomedical Optics, no. 11, pp. 041112-1 - 041112-11, 2006
    BibTeX
    @article{Neumann,
       author = {Neumann, J. and Brinkmann, R},
       title = {Cell disintegration by laser-induced transient microbubbles and its simultaneous monitoring by interferometry},
       journal = {Journal of Biomedical Optics},
       volume = {11},
       number = {4},
       pages = {041112-1 - 041112-11},
       year = {2006}
    }
  • Klatt, C. and Elsner, H. and Porksen, E. and Brinkmann, R. and Bunse, A. and Birngruber, R. and Roider, J.: Selektive Retina-Therapie bei Retinopathia centralis serosa mit Pigmentepithelabhebung. Ophthalmologe, no. 103, pp. 850-855, Aug, 2006
    BibTeX
    @article{Klatt,
       author = {Klatt, C. and Elsner, H. and Porksen, E. and Brinkmann, R. and Bunse, A. and Birngruber, R. and Roider, J.},
       title = {Selektive Retina-Therapie bei Retinopathia centralis serosa mit Pigmentepithelabhebung},
       journal = {Ophthalmologe},
       volume = {103},
       number = {10},
       pages = {850-855},
       note = {0941-293X (Print)
    Clinical Trial
    English Abstract
    Journal Article},
       month = {Aug},
       abstract = {Selective Retina Therapy (SRT) is a new laser treatment that selectively targets the retinal pigmen epithelium (RPE). In this study, we treated 39 patients presenting with nonischemic, focal and focal-diffuse diabetic maculopathy with SRT. In the main. the results indicate that SRT had stabilizing effects on visual acuity, angiographic leakage, lipid exudation, and foveal retinal thickness. SRT is safe and is especially useful for treating pathologies that are located close to the fovea, which cannot be treated with conventional argon laser photocoagulation.},
       keywords = {Aged
    Diabetic Retinopathy/*surgery
    Female
    Humans
    Laser Surgery/*methods
    Lasers/*therapeutic use
    Macular Degeneration/*surgery
    Male
    Ophthalmologic Surgical Procedures/*methods
    Preoperative Care/methods
    Treatment Outcome},
       year = {2006}
    }
  • Kandulla, J. and Elsner, H. and Birngruber, R. and Brinkmann, R.: Noninvasive optoacoustic online retinal temperature determination during continuous-wave laser irradiation. J Biomed Opt, no. 11, pp. 041111, 2006
    BibTeX
    @article{Kandulla,
       author = {Kandulla, J. and Elsner, H. and Birngruber, R. and Brinkmann, R.},
       title = {Noninvasive optoacoustic online retinal temperature determination during continuous-wave laser irradiation},
       journal = {J Biomed Opt},
       volume = {11},
       number = {4},
       pages = {041111},
       note = {1083-3668 (Print)
    In Vitro
    Journal Article
    Research Support, Non-U.S. Gov't},
       abstract = {The therapeutic effect of most retinal laser treatments is initiated by a transient temperature increase. Although crucial to the effectiveness of the treatment, the temperature course is not exactly known due to individually different tissue properties. We develop an optoacoustic method to determine the retinal temperature increase in real time during continuous-wave (cw) laser irradiation, and perform temperature calculations to interpret the results exemplary for transpupillary thermotherapy (TTT). Porcine globes ex vivo and rabbit eyes in vivo are irradiated with a diode laser (lambda=810 nm, P< or =3 W, phi=2 mm) for 60 s. Simultaneously, pulses from a N2-laser pumped dye laser (lambda=500 nm, tau=3.5 ns, E approximately 5 microJ) are applied on the retina. Following its absorption, an ultrasonic pressure wave is emitted, which is detected by a transducer embedded in a contact lens. Using the previously measured temperature-dependent Gruneisen coefficient of chorioretinal tissue, a temperature raise in porcine eyes of 5.8 degrees C(Wcm2) after 60 s is observed and confirmed by simultaneous measurements with an inserted thermocouple. In a rabbit, we find 1.4 degrees C(Wcm2) with, and 2.2 degrees C(Wcm2) without perfusion at the same location. Coagulation of the rabbit's retina occurs at DeltaT=21 degrees C after 40 s. In conclusion, this optoacoustic method seems feasible for an in vivo real-time determination of temperature, opening the possibility for feedback control retinal laser treatments.},
       keywords = {Animals
    Body Temperature/physiology/radiation effects
    Equipment Design
    Equipment Failure Analysis
    Feasibility Studies
    Hyperthermia, Induced/*instrumentation
    Lasers/*therapeutic use
    Online Systems
    Rabbits
    Reproducibility of Results
    Retina/*physiology/*radiation effects/ultrasonography
    Sensitivity and Specificity
    Swine
    Thermography/*instrumentation/methods
    Ultrasonography/*instrumentation/methods},
       year = {2006}
    }
  • Elsner, H and Pörksen, E and Klatt, C and Bunse, A and Theisen-Kunde, D and Brinkmann, R and Birngruber, R and Laqua, H and Roider, J: Selective Retina Therapy (SRT) in patients with central serous chorioretinopathy (CSC). Graefes Arch Ophthalmol, in print., no. on-line: DOI 10.1007/s00417-006-0368-5, 2006
    BibTeX
    @article{Elsner,
       author = {Elsner, H and Pörksen, E and Klatt, C and Bunse, A and Theisen-Kunde, D and Brinkmann, R and Birngruber, R and Laqua, H and Roider, J},
       title = {Selective Retina Therapy (SRT) in patients with central serous chorioretinopathy (CSC)},
       journal = {Graefes Arch Ophthalmol, in print.},
       volume = {on-line: DOI 10.1007/s00417-006-0368-5},
       year = {2006}
    }
  • Huber, Robert and Adler, Desmond C and Fujimoto, James G: Buffered Fourier domain mode locking: unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s. Optics Letters, no. 31, pp. 2975 - 2977, oct,, 2006
    BibTeX Link
    @Article{HU_2006_Huber_b,
      Title                    = {{Buffered Fourier domain mode locking: unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s}},
      Author                   = {Huber, Robert and Adler, Desmond C and Fujimoto, James G},
      Journal                  = {Optics Letters},
      Year                     = {2006},
    
      Month                    = oct,
      Number                   = {20},
      Pages                    = {2975 -- 2977 },
      Volume                   = {31},
    
      Doi                      = {10.1364/OL.31.002975},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-31-20-2975}
    }
  • Lenz, Martin O and Huber, Robert and Schmidt, Bernhard and Gilch, Peter and Kalmbach, Rolf and Engelhard, Martin and Wachtveitl, Josef: First steps of retinal photoisomerization in proteorhodopsin. Biophysical journal, no. 91, pp. 255-62, jul,, 2006
    BibTeX Link
    @Article{HU_2006_Lenz_a,
      Title                    = {{First steps of retinal photoisomerization in proteorhodopsin}},
      Author                   = {Lenz, Martin O and Huber, Robert and Schmidt, Bernhard and Gilch, Peter and Kalmbach, Rolf and Engelhard, Martin and Wachtveitl, Josef},
      Journal                  = {Biophysical journal},
      Year                     = {2006},
    
      Month                    = jul,
      Number                   = {1},
      Pages                    = {255--62},
      Volume                   = {91},
    
      Doi                      = {10.1529/biophysj.105.074690},
      Institution              = {Institut f\"{u}r Physikalische und Theoretische Chemie, Johann-Wolfgang-Goethe-Universit\"{a}t, Frankfurt, Germany.},
      ISSN                     = {0006-3495},
      Url                      = {http://www.cell.com/biophysj/abstract/S0006-3495\%2806\%2971725-8}
    }
  • Huber, R and Wojtkowski, M and Fujimoto, J G: Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography. Optics Express, no. 14, pp. 3225 - 3237, apr,, 2006
    BibTeX Link
    @Article{HU_2006_Huber_c,
      Title                    = {{Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography}},
      Author                   = {Huber, R and Wojtkowski, M and Fujimoto, J G},
      Journal                  = {Optics Express},
      Year                     = {2006},
    
      Month                    = apr,
      Number                   = {8},
      Pages                    = {3225 -- 3237 },
      Volume                   = {14},
    
      Doi                      = {10.1364/OE.14.003225},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-8-3225}
    }
  • Huber, R and Wojtkowski, M and Fujimoto, J G: Fourier domain mode locking (FDML): Three-dimensional OCT imaging at 906 frames per second. in Conference on Lasers and Electro-Optics and 2006 Quantum Electronics and Laser Science Conference, CLEO/QELS 2006, 2006
    BibTeX Link
    @InProceedings{HU_2006_Huber_d,
      Title                    = {{Fourier domain mode locking (FDML): Three-dimensional OCT imaging at 906 frames per second}},
      Author                   = {Huber, R and Wojtkowski, M and Fujimoto, J G},
      Booktitle                = {Conference on Lasers and Electro-Optics and 2006 Quantum Electronics and Laser Science Conference, CLEO/QELS 2006},
      Year                     = {2006},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=CLEO-2006-CMZ2}
    }
  • Huber, R and Taira, K and Wojtkowski, M and Fujimoto, J G: Fourier domain mode-locked lasers for swept source OCT imaging at up to 290 kHz scan rates. in Proceedings of SPIE, no. 6079, pp. 60790U-60790U-6, feb,, 2006
    BibTeX Link
    @InProceedings{HU_2006_Huber_a,
      Title                    = {{Fourier domain mode-locked lasers for swept source OCT imaging at up to 290 kHz scan rates}},
      Author                   = {Huber, R and Taira, K and Wojtkowski, M and Fujimoto, J G},
      Booktitle                = {Proceedings of SPIE},
      Year                     = {2006},
      Editor                   = {Tuchin, Valery V. and Izatt, Joseph A. and Fujimoto, James G.},
      Month                    = feb,
      Pages                    = {60790U--60790U--6},
      Volume                   = {6079},
    
      Doi                      = {10.1117/12.648880},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1273744}
    }
  • Kranendonk, Laura A and Walewski, Joachim W and Sanders, Scott T and Huber, Robert and Fujimoto, James G: Measurements of Gas Temperature in a HCCI Engine Using a Fourier Domain Mode Locking Laser. pp. 1366 1-5, apr,, 2006
    BibTeX Link
    @TechReport{HU_2006_Kranendonk_a,
      Title                    = {{Measurements of Gas Temperature in a HCCI Engine Using a Fourier Domain Mode Locking Laser}},
      Author                   = {Kranendonk, Laura A and Walewski, Joachim W and Sanders, Scott T and Huber, Robert and Fujimoto, James G},
      Year                     = {2006},
      Month                    = apr,
      pages  = {1366 1--5}
    
      Doi                      = {10.4271/2006-01-1366},
      Url                      = {http://www.sae.org/technical/papers/2006-01-1366}
    }
  • Kranendonk, Laura A and Walewski, Joachim W and Sanders, Scott T and Huber, Robert and Fujimoto, James G: Measurements of Gas Temperature in an HCCI Engine by Use of a Fourier-Domain Mode-Locking Laser. in Laser Applilcations to Chemical, Security and Environmental Analysis, pp. TuB2 1-2, OSA, Washington, D.C., 2006
    BibTeX Link
    @InProceedings{HU_2006_Kranendonk_b,
      Title                    = {{Measurements of Gas Temperature in an HCCI Engine by Use of a Fourier-Domain Mode-Locking Laser}},
      Author                   = {Kranendonk, Laura A and Walewski, Joachim W and Sanders, Scott T and Huber, Robert and Fujimoto, James G},
      Booktitle                = {Laser Applilcations to Chemical, Security and Environmental Analysis},
      Year                     = {2006},
    
      Address                  = {Washington, D.C.},
      Pages                    = {TuB2 1--2 },
      Publisher                = {OSA},
    
      Doi                      = {10.1364/LACSEA.2006.TuB2},
      ISBN                     = {1-55752-799-7},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=LACSEA-2006-TuB2}
    }

2005

  • Schmidt-Erfurth, U. and Michels, S. and Indorf, L. and Eggers, R. and Birngruber, R.: Mechanism of photodynamic occlusion using liposomal Zn(II)-phtalocyanine. Current Eye Research, no. 30, pp. 601-612, 2005
    BibTeX Link
    @article{Schmidt-Erfurth2005,
       author = {Schmidt-Erfurth, U. and Michels, S. and Indorf, L. and Eggers, R. and Birngruber, R.},
       title = {Mechanism of photodynamic occlusion using liposomal Zn(II)-phtalocyanine},
       journal = {Current Eye Research},
       volume = {30},
       number = {7},
       pages = {601-612},
       note = {953LO
    Times Cited:2
    Cited References Count:30},
       abstract = {Purpose: To evaluate the potential of liposomal Zinc(II)phthalocyanine (ZnPc) to selectively target subretinal vasculature. Methods: Photodynamic therapy (PDT) with liposomal Zinc(II)-phtalocyanine was used to induce choroidal occlusion in eyes of pigmented rabbits. Drug doses of 0.16, 0.24, 0.32, and 0.4 mg/kg body weight were administered. Photosensitization was performed at a wavelength of 671 nm and an irradiance of 100 mW/cm(2) applying fluences of 5, 10, 20, and 50 J/cm(2). Results: Using liposomal ZnPc, occlusion of choroidal vessels was achieved without damage to the overlying neurosensory retina. A tight dose correlation was found with a drug dose of 0.32 mg/kg and a light dose of 10 J/cm(2) inducing a selective thrombosis of the subretinal capillary layer. Histology revealed a selective intravascular alteration of the endothelial cells. Conclusions: PDT using liposomal ZnPc allows occlusion of subretinal vasculature with maintenance of neuroretina and RPE. The destructive effect on choroidal vascular endothelium is intensive.},
       keywords = {choroidal vasculature
    liposomes
    photodynamic therapy
    zinc(ii)-phtalocyanine
    low-density-lipoprotein
    receptor-mediated uptake
    choroidal neovascularization
    macular degeneration
    endothelial-cells
    therapy
    verteporfin
    angiography
    model},
       ISSN = {0271-3683},
       DOI = {Doi 10.1080/02713680590968286},
       url = {<Go to ISI>://WOS:000231078000012},
       year = {2005},
       type = {Journal Article}
    }
    
  • Poerksen, E. and Elsner, H. and Theisen-Kunde, D. and Schuele, G. and Hamilton, P. and Laqua, H. and Birngruber, R. and Brinkmann, R. and Roider, J.: Selective retina treatment (SRT): Clinical investigation of an optoacoustic on-line dosimetry control. Investigative Ophthalmology & Visual Science, no. 46, 2005
    BibTeX Link
    @article{Poerksen2005,
       author = {Poerksen, E. and Elsner, H. and Theisen-Kunde, D. and Schuele, G. and Hamilton, P. and Laqua, H. and Birngruber, R. and Brinkmann, R. and Roider, J.},
       title = {Selective retina treatment (SRT): Clinical investigation of an optoacoustic on-line dosimetry control},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {46},
       note = {Suppl. S
    911CZ
    294
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000227980400286},
       year = {2005},
       type = {Journal Article}
    }
    
  • Neumann, J and Brinkmann, R: Boiling nucleation on melanosomes and microbeads transiently heated by nanosecond and microsecond laser pulses.. J Biomed Optics, no. 10, pp. 024001, 2005
    BibTeX
    @article{Neumann,
       author = {Neumann, J and Brinkmann, R},
       title = {Boiling nucleation on melanosomes and microbeads transiently heated by nanosecond and microsecond laser pulses.},
       journal = {J Biomed Optics},
       volume = {10},
       number = {2},
       pages = {024001},
       year = {2005}
    }
  • Herrmann, K and Birngruber, R. and Pauleikhoff, D.: Photodynamische Therapie in der Praxis immer breiter eingesetzt.. Beilage für Der Ophthalmologe, no. 102, 2005
    BibTeX
    @article{Herrmann2005,
       author = {Herrmann, K and Birngruber, R. and Pauleikhoff, D.},
       title = {Photodynamische Therapie in der Praxis immer breiter eingesetzt.},
       journal = {Beilage für "Der Ophthalmologe"},
       volume = {102},
       number = {6},
       year = {2005},
       type = {Journal Article}
    }
    
  • Geerling, G. and Muller, M. and Winter, C. and Hoerauf, H. and Oelckers, S. and Laqua, H. and Birngruber, R.: Intraoperative 2-dimensional optical coherence tomography as a new tool for anterior segment surgery. Arch Ophthalmol, no. 123, pp. 253-7, 2005
    BibTeX Link
    @article{Geerling2005,
       author = {Geerling, G. and Muller, M. and Winter, C. and Hoerauf, H. and Oelckers, S. and Laqua, H. and Birngruber, R.},
       title = {Intraoperative 2-dimensional optical coherence tomography as a new tool for anterior segment surgery},
       journal = {Arch Ophthalmol},
       volume = {123},
       number = {2},
       pages = {253-7},
       note = {0003-9950 (Print)
    Journal Article},
       abstract = {We tested a 1310-nm optical coherence tomograph coupled with a beam splitter on the front lens of an operating microscope for intraoperative, noncontact visualization of anterior segment procedures. This new modification of optical coherence tomography technology allows intraoperative, high-resolution, cross-sectional imaging and pachymetry of the cornea and sclera during anterior segment surgery. It is particularly helpful for lamellar dissection techniques such as deep anterior lamellar keratoplasty and trabeculectomy.},
       keywords = {Anterior Eye Segment/*surgery
    Cornea/pathology
    Corneal Transplantation/methods
    Diagnostic Techniques, Ophthalmological/*instrumentation
    Humans
    Monitoring, Intraoperative/*instrumentation
    *Ophthalmologic Surgical Procedures
    Sclera/pathology
    Tomography, Optical Coherence/*instrumentation
    Trabecular Meshwork/pathology
    Trabeculectomy/methods},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15710824},
       year = {2005},
       type = {Journal Article}
    }
    
  • Framme, C. and Schuele, G. and Roider, J. and Holz, F. G. and Birngruber, R. and Brinkmann, R.: Temperature dependence of A2-E fluorescence in vitro and detection of fundus autofluorescence during selective RPE laser treatment (SRT). Investigative Ophthalmology & Visual Science, no. 46, 2005
    BibTeX Link
    @article{Framme2005,
       author = {Framme, C. and Schuele, G. and Roider, J. and Holz, F. G. and Birngruber, R. and Brinkmann, R.},
       title = {Temperature dependence of A2-E fluorescence in vitro and detection of fundus autofluorescence during selective RPE laser treatment (SRT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {46},
       note = {Suppl. S
    911CZ
    266
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000227980400260},
       year = {2005},
       type = {Journal Article}
    }
    
  • Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.: Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles. J Biomed Opt, no. 10, pp. 064012, 2005
    BibTeX
    @article{Yao,
       author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.},
       title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles},
       journal = {J Biomed Opt},
       volume = {10},
       number = {6},
       pages = {064012},
       note = {Yao, Cuiping
    Rahmanzadeh, Ramtin
    Endl, Elmar
    Zhang, Zhenxi
    Gerdes, Johannes
    Huttmann, Gereon
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2005 Nov-Dec;10(6):064012.},
       abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.},
       keywords = {Biopolymers/pharmacokinetics
    Cell Line, Tumor
    Cell Membrane Permeability/ physiology/ radiation effects
    Drug Delivery Systems/ methods
    Fluoresceins/ pharmacokinetics
    Humans
    Lasers
    Lymphoma/ metabolism
    Nanostructures},
       year = {2005}
    }
  • Rahmanzadeh, R. and Muller, K. and Fischer, E. and Bartels, D. and Borsch, T.: The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales). no. 7, pp. 67-78, Jan, 2005
    BibTeX
    @misc{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Muller, K. and Fischer, E. and Bartels, D. and Borsch, T.},
       title = {The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales)},
       volume = {7},
       number = {1},
       pages = {67-78},
       month = {Jan},
       note = {Rahmanzadeh, R
    Muller, K
    Fischer, E
    Bartels, D
    Borsch, T
    Research Support, Non-U.S. Gov't
    Germany
    Plant Biol (Stuttg). 2005 Jan;7(1):67-78.},
       abstract = {The Lamiales are one of the largest orders of angiosperms, with about 22,000 species. The Scrophulariaceae, as one of their most important families, has recently been shown to be polyphyletic. As a consequence, this family was re-classified and several groups of former scrophulariaceous genera now belong to different families, such as the Calceolariaceae, Plantaginaceae, or Phrymaceae. In the present study, relationships of the genera Craterostigma, Lindernia and its allies, hitherto classified within the Scrophulariaceae, were analyzed. Sequences of the chloroplast trnK intron and the matK gene (approximately 2.5 kb) were generated for representatives of all major lineages of the Lamiales and the former Scrophulariaceae. Bayesian and parsimony analyses revealed two isolated lineages, one of which consists of Lindernia and its allies, the other of Gratiola and allies. Gratiola was previously assumed to be related to Lindernia and was therefore included here. It is proposed to treat the two clades as separate families, Linderniaceae and Gratiolaceae. For the Linderniaceae, several morphological synapomorphies exist in addition to molecular data, such as conspicuous club-shaped stamen appendages.},
       keywords = {Bayes Theorem
    Genes, Plant
    Introns
    Phenotype
    Phylogeny
    Scrophulariaceae/anatomy & histology/ classification/genetics
    Species Specificity},
       ISBN = {1435-8603 (Print)
    1435-8603 (Linking)},
       year = {2005}
    }
  • Yamaguchi, M and Yokoyama, T and Tamaoki, S and Hayashi, Y and Tabuchi, H and Shiraki, K: Lateral rectus posterior-fixation suture for large-angle exotropia. Japanese Review of Clinical Ophthalmology, no. 99, pp. 307-311, 2005
    BibTeX
    @article{Hayashi,
       author = {Yamaguchi, M and Yokoyama, T and Tamaoki, S and Hayashi, Y and Tabuchi, H and Shiraki, K},
       title = {Lateral rectus posterior-fixation suture for large-angle exotropia},
       journal = {Japanese Review of Clinical Ophthalmology},
       volume = {99},
       number = {4},
       pages = {307-311},
       year = {2005}
    }
    
  • Schuele, G. and Elsner, H. and Framme, C. and Roider, J. and Birngruber, R. and Brinkmann, R.: Optoacoustic real-time dosimetry for selective retina treatment. J Biomed Opt, no. 10, pp. 064022, 2005
    BibTeX
    @article{Schuele,
       author = {Schuele, G. and Elsner, H. and Framme, C. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Optoacoustic real-time dosimetry for selective retina treatment},
       journal = {J Biomed Opt},
       volume = {10},
       number = {6},
       pages = {064022},
       note = {1083-3668 (Print)
    Journal Article},
       abstract = {The selective retina treatment (SRT) targets retinal diseases associated with disorders in the retinal pigment epithelium (RPE). Due to the ophthalmoscopic invisibility of the laser-induced RPE effects, we investigate a noninvasive optoacoustic real-time dosimetry system. In vitro porcine RPE is irradiated with a Nd:YLF laser (527 nm, 1.7-micros pulse duration, 5 to 40 microJ, 30 pulses, 100-Hz repetition rate). Generated acoustic transients are measured with a piezoelectric transducer. During 27 patient treatments, the acoustic transients are measured with a transducer embedded in an ophthalmic contact lens. After treatment, RPE damage is visualized by fluorescein angiographic leakage. Below the RPE damage threshold, the optoacoustic transients show no pulse-to-pulse fluctuations within a laser pulse train. Above threshold, fluctuations of the individual transients among each other are observed. If optoacoustic pulse-to-pulse fluctuations are present, RPE leakage is observed in fluorescein angiography. In 96% of the irradiated areas, RPE leakage correlated with the optoacoustic defined threshold value. A noninvasive optoacoustic real-time dosimetry for SRT is developed and proved in vitro and during patient treatment. It detects the ophthalmoscopically invisible laser-induced damage of RPE cells and overcomes practical limitations of SRT for use in private practice.},
       keywords = {Acoustics/*instrumentation
    Animals
    Computer Systems
    Dose-Response Relationship, Radiation
    Equipment Design
    Equipment Failure Analysis
    Humans
    Laser Coagulation/*methods
    Lasers/*therapeutic use
    Optics/*instrumentation
    Radiation Dosage
    Radiometry/*instrumentation/methods
    Reproducibility of Results
    Retina/pathology/*radiation effects/*surgery
    Sensitivity and Specificity
    Swine},
       year = {2005}
    }
  • Vogel, A and Lorenz, B. and Sägmüller, B. and Schütze, K.: Catapulting of microdissected histologic specimens with focused and defocused laser pulses. Optical Society of America, 2005
    BibTeX
    @article{Vogel,
       author = {Vogel, A and Lorenz, B. and Sägmüller, B. and Schütze, K.},
       title = {Catapulting of microdissected histologic specimens with focused and defocused laser pulses},
       journal = {Optical Society of America},
       year = {2005}
    }
  • Kasahara, F and Yokoyama, T and Hayashi, Y and Kato, Y and Yamaguchi, M and Shiraki, K: Postoperative results of distance stereoacuity in intermittent exotropia. Japanese Review of Clinical Ophthalmology, no. 99, pp. 246-249, 2005
    BibTeX
    @article{Hayashi,
       author = {Kasahara, F and Yokoyama, T and Hayashi, Y and Kato, Y and Yamaguchi, M and Shiraki, K},
       title = {Postoperative results of distance stereoacuity in intermittent exotropia},
       journal = {Japanese Review of Clinical Ophthalmology},
       volume = {99},
       number = {3},
       pages = {246-249},
       year = {2005}
    }
    
  • Schüle, G and Rumohr, M and Hüttmann, G and Brinkmann, R: RPE damage thresholds and mechanisms for laser exposure in the µs to ms time regimen. Inv Ophthalmol & Vis Sci, no. 46, pp. 714-719, 2005
    BibTeX
    @article{Schüle,
       author = {Schüle, G and Rumohr, M and Hüttmann, G and Brinkmann, R},
       title = {RPE damage thresholds and mechanisms for laser exposure in the µs to ms time regimen},
       journal = {Inv Ophthalmol & Vis Sci},
       volume = {46},
       number = {2},
       pages = {714-719},
       year = {2005}
    }
  • Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.: Mechanisms of femtosecond laser nanosurgery of biological cells and tissues. Appl. Phys B, no. 81, pp. 1015-1047, 2005
    BibTeX
    @article{Vogel2005,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Mechanisms of femtosecond laser nanosurgery of biological cells and tissues},
       journal = {Appl. Phys B},
       volume = {81},
       pages = {1015-1047},
       year = {2005}
    }
  • Hüttmann, Gereon and Yao, Cuiping and Endl, Elmar: New concepts in laser medicine: Towards a laser surgery with cellular precision. Medical laser application, no. 20, pp. 135-139, 2005
    BibTeX
    @article{Hüttmann2005,
       author = {Hüttmann, Gereon and Yao, Cuiping and Endl, Elmar},
       title = {New concepts in laser medicine: Towards a laser surgery with cellular precision},
       journal = {Medical laser application},
       volume = {20},
       number = {2},
       pages = {135-139},
       abstract = {New concepts and instrumentation in laser medicine are driven by the progress in optical technology as well as by advances in the understanding of the interaction of optical irradiation with tissue, especially at a macromolecular scale, and by the changing needs in health care. Complexity and costs of laser sources will decrease due to the use of semiconductor and fiber lasers, and complex or non-linear mechanisms by which the radiation effects tissues are better understood, especially when ultra-short laser pulses are used. Especially femtosecond lasers and nanotechnology have the potential to treat diseases at a cellular level. Focused femtosecond irradiation was successfully used to manipulate tissues with subcellular precision. Laser-irradiated nanoparticles can selectively destroy individual cells.},
       keywords = {Mikro- und Nanowirkungen
    Selective Foto thermolyse
    Faserlaser
    Femtosekundenlaser
    Nanopartikel
    Micro- and nanoeffects
    Selective photothermolysis
    Fiber laser
    Femtosecond laser
    Nanoparticles},
       year = {2005}
    }
  • Daniltchenko, D. and Sachs, M. and Lankenau, E. and König, F. and Burckhardt, M. and Hüttmann, G. and Kristiansen, G. and Schnorr, D. and Al-Shukri, S. and Löning, S.: Ex vivo and in vivo topographics studies of bladder by optical coherence tomography. in Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, no. 5771, pp. 209-14, Proceedings 2005,
    BibTeX
    @inproceedings{Daniltchenko,
       author = {Daniltchenko, D. and Sachs, M. and Lankenau, E. and König, F. and Burckhardt, M. and Hüttmann, G. and Kristiansen, G. and Schnorr, D. and Al-Shukri, S. and Löning, S.},
       title = {Ex vivo and in vivo topographics studies of bladder by optical coherence tomography},
       booktitle = {Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI},
       editor = {Tuchin, V.},
       publisher = {Proceedings 2005},
       volume = {5771},
       pages = {209-14},
    
    }
  • Framme, C and Roider, J and Sachs, H G and Brinkmann, R and Gabel, V-P: Noninvasive Imaging and Monitoring of Retinal Pigment Epithelium Patterns Using Fundus Autofluorescence - Review. Curr Med Imag Rev, no. 1, pp. 89-103, 2005
    BibTeX
    @article{Framme,
       author = {Framme, C and Roider, J and Sachs, H G and Brinkmann, R and Gabel, V-P},
       title = {Noninvasive Imaging and Monitoring of Retinal Pigment Epithelium Patterns Using Fundus Autofluorescence - Review},
       journal = {Curr Med Imag Rev},
       volume = {1},
       pages = {89-103},
       year = {2005}
    }
  • Apitz, I. and Vogel, A.: Material ejection in nanosecond Er:YAG laser ablation of water, liver, and skin. Applied Physics A: Materials Science & Processing, no. 81, pp. 329-338, 2005
    BibTeX
    @article{Apitz,
       author = {Apitz, I. and Vogel, A.},
       title = {Material ejection in nanosecond Er:YAG laser ablation of water, liver, and skin},
       journal = {Applied Physics A: Materials Science & Processing},
       volume = {81},
       number = {2},
       pages = {329-338},
       abstract = {We investigated the mechanisms of material ejection in Q-switched Er:YAG laser tissue ablation (70-ns pulse duration) where moderate and large radiant exposures are associated with large volumetric energy densities in the target material. For water, an initial phase of non-equilibrium surface vaporization is followed by an explosive vaporization of the superficial liquid volume from a supercritical state. The ablation of deeper layers with lower peak temperatures proceeds as phase explosion. For mechanically strong tissues, non-equilibrium surface vaporization is followed by a vapour explosion coupled with thermal dissociation of the biomolecules into volatile products. In deeper layers, ablation proceeds as confined boiling with mechanical tearing of the tissue matrix by the vapour pressure. The recoil stress induced at a radiant exposure of 5.4 J/cm 2 is in the order of 500–900 MPa. For water and soft tissues such as liver, the recoil causes a powerful secondary material expulsion. For stronger tissues such as skin, no secondary expulsion was observed even though the recoil stress largely exceeds the static tensile strength of the tissue. Recoil-induced material expulsion results in an increase of both ablation efficiency and mechanical side effects of ablation. Theoretical modelling of the succession of phase transitions in nanosecond-laser tissue ablation and of recoil-induced material expulsion remain a major challenge for future work.},
       keywords = {Physik und Astronomie},
       year = {2005}
    }
  • Alt, C. and Framme, C. and Schnell, S. and Lee, H. and Brinkmann, R. and Lin, C. P.: Selective targeting of the retinal pigment epithelium using an acousto-optic laser scanner. J Biomed Opt, no. 10, pp. 64014, 2005
    BibTeX
    @article{Alt,
       author = {Alt, C. and Framme, C. and Schnell, S. and Lee, H. and Brinkmann, R. and Lin, C. P.},
       title = {Selective targeting of the retinal pigment epithelium using an acousto-optic laser scanner},
       journal = {J Biomed Opt},
       volume = {10},
       number = {6},
       pages = {64014},
       note = {1083-3668 (Print)
    Journal Article},
       abstract = {Selective targeting of the retinal pigment epithelium (RPE) is a new strategy for treating certain retinal disorders while preserving adjacent photoreceptors. The treatment currently relies on a complex laser system to produce the required microsecond pulse structure. In our new approach, we scan the focus of a continuous-wave (cw) laser beam with acousto-optic deflectors to produce microsecond-long exposures at each RPE cell. Experiments were performed in vitro with a bench-top scanner on samples of young bovine RPE and in vivo on Dutch belted rabbits with a slit-lamp adapted scanner. Effective dose 50% (ED(50)) for RPE damage was determined in vitro by fluorescence cell viability assay and in vivo by fluorescein angiography. Damage to individual RPE cells was achieved with laser power on the order of 100 mW. Using separated scan lines, we demonstrate selectivity in the form of alternating lines of dead and surviving cells that resemble the scan pattern. Selectivity is also shown by the absence of retinal thermal coagulation in vivo. Selective RPE damage is feasible by rapidly scanning a cw laser beam. The scanning device is an attractive alternative to conventional laser coagulation and pulsed laser targeting of the RPE.},
       year = {2005}
    }
  • Kandulla, J and Elsner, H and Hilmes, M and Hartert, C and Brinkmann, R: Optoacoustic temperature determination at the fundus of the eye during Transpupillary Thermotherapy. in Proc SPIE, no. 5688, pp. 208-214,
    BibTeX
    @inproceedings{Kandulla2005,
       author = {Kandulla, J and Elsner, H and Hilmes, M and Hartert, C and Brinkmann, R},
       title = {Optoacoustic temperature determination at the fundus of the eye during Transpupillary Thermotherapy},
       booktitle = {Proc SPIE},
       volume = {5688},
       pages = {208-214},
       keywords = {AutoPhoN},
       type = {Conference Proceedings}
    }
    
  • Nakakura, S. and Shiraki, K. and Yasunari, T. and Hayashi, Y. and Ataka, S. and Kohno, T.: Quantification and anatomic distribution of choroidal abnormalities in patients with type I neurofibromatosis. Graefes Arch Clin Exp Ophthalmol, no. 243, pp. 980-4, 2005
    BibTeX
    @article{Hayashi,
       author = {Nakakura, S. and Shiraki, K. and Yasunari, T. and Hayashi, Y. and Ataka, S. and Kohno, T.},
       title = {Quantification and anatomic distribution of choroidal abnormalities in patients with type I neurofibromatosis},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {243},
       number = {10},
       pages = {980-4},
       note = {Nakakura, Shunsuke
    Shiraki, Kunihiko
    Yasunari, Takaharu
    Hayashi, Yoko
    Ataka, Shinsuke
    Kohno, Takeya
    eng
    Comparative Study
    Germany
    2005/05/14 09:00
    Graefes Arch Clin Exp Ophthalmol. 2005 Oct;243(10):980-4. Epub 2005 Oct 20.},
       abstract = {BACKGROUND: Choroidal abnormality manifesting as a bright patchy lesion under infrared monochromatic light has previously been described in neurofibromatosis type I patients in whom the choroid appears normal under conventional ophthalmoscopic examination or on the fluorescein angiogram. We investigated the correlation between patient age and the number of choroidal abnormalities, as well as the anatomic distribution of choroidal abnormalities in the fundus. METHODS: We examined the fundus of 28 eyes in 14 patients with neurofibromatosis type I. Patients ranged in age from 2 to 38 years and were examined between April 2001 and April 2002 by confocal scanning laser ophthalmoscopy with infrared monochromatic light (780 nm wavelength). We divided the fundus into five regions (one within the retinal vascular arcade and those supero-temporal, infero-temporal, supero-nasal, and infero-nasal to it), and lesions on the border between regions were assigned to the region containing the greater part of the lesion. We studied the total number of choroidal abnormalities and the correlation between the total number and age. RESULTS: A positive correlation was found between the total number of choroidal abnormalities and age (Spearman rank correlation coefficient, r=0.6209, P=0.0178). There was a significantly greater number of choroidal abnormalities in the arcade region than in the other four regions (ANOVA, P<0.001). CONCLUSIONS: Choroidal abnormalities tend to increase with age and are most often observed within the vascular arcade.},
       keywords = {Adolescent
    Adult
    Child
    Child, Preschool
    Choroid/*abnormalities
    Choroid Diseases/complications/epidemiology/*pathology
    Female
    Fundus Oculi
    Humans
    Lasers/diagnostic use
    Male
    Neurofibromatosis 1/*complications/pathology
    Ophthalmoscopy/methods
    Prevalence
    Retrospective Studies},
       year = {2005}
    }
    
  • Hamaguchi, A and Hayashi, Y and Motoyama, T and Imamura, H and Imamoto, K and Kohno, T and Shiraki, K: A case of bilateral endogenous bacterial endophthalmitis. Folia ophthalmologica Japonica, no. 56, pp. 826-830, 2005
    BibTeX
    @article{Hamaguchi,
       author = {Hamaguchi, A and Hayashi, Y and Motoyama, T and Imamura, H and Imamoto, K and Kohno, T and Shiraki, K},
       title = {A case of bilateral endogenous bacterial endophthalmitis},
       journal = {Folia ophthalmologica Japonica},
       volume = {56},
       number = {10},
       pages = {826-830},
       year = {2005}
    }
    
  • Horneffer, V. and Vogel, A. and Sägmüller, B. and Schütze, K.: Microdissection, catapulting, and microinjection of biologic specimens with femtosecond laser pulses. in SPIE/OSA Conference on Biomedical Optics ECBO,12.-16.06.2005,
    BibTeX
    @inproceedings{Horneffer,
       author = {Horneffer, V. and Vogel, A. and Sägmüller, B. and Schütze, K.},
       title = {Microdissection, catapulting, and microinjection of biologic specimens with femtosecond laser pulses},
       booktitle = {SPIE/OSA Conference on Biomedical Optics ECBO,12.-16.06.2005},
    }
  • Elsner, H. and Liew, S. H. M. and Klatt, C. and Hamilton, P. and Marshall, J. and Porksen, E. and Laqua, H. and Brinkmann, R. and Birngruber, R. and Roider, J.: Selective-retina-therapy (SRT) multicenter clinical trial: 6 month results in patients with diabetic maculopathy. Investigative Ophthalmology & Visual Science, no. 46, 2005
    BibTeX Link
    @article{Elsner2005,
       author = {Elsner, H. and Liew, S. H. M. and Klatt, C. and Hamilton, P. and Marshall, J. and Porksen, E. and Laqua, H. and Brinkmann, R. and Birngruber, R. and Roider, J.},
       title = {Selective-retina-therapy (SRT) multicenter clinical trial: 6 month results in patients with diabetic maculopathy},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {46},
       note = {Suppl. S
    911CZ
    1463
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000227980401487},
       year = {2005},
       type = {Journal Article}
    }
    
  • Huber, R and Wojtkowski, M and Taira, K and Fujimoto, J. G. and Hsu, K: Amplified, frequency swept lasers for frequency domain reflectometry and OCT imaging: design and scaling principles. Optics Express, no. 13, pp. 3513 - 3528, may,, 2005
    BibTeX Link
    @Article{HU_2005_Huber_c,
      Title                    = {{Amplified, frequency swept lasers for frequency domain reflectometry and OCT imaging: design and scaling principles}},
      Author                   = {Huber, R and Wojtkowski, M and Taira, K and Fujimoto, J. G. and Hsu, K},
      Journal                  = {Optics Express},
      Year                     = {2005},
    
      Month                    = may,
      Number                   = {9},
      Pages                    = {3513 -- 3528 },
      Volume                   = {13},
    
      Doi                      = {10.1364/OPEX.13.003513},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-9-3513}
    }
  • Huber, R and Taira, K and Wojtkowski, M and Fujimoto, J G: Fourier domain mode locked lasers for OCT imaging at up to 290 kHz sweep rates. in European Conference on Biomedical Optics 2005, pp. 58611B-58611B-6, International Society for Optics and Photonics, jun,, 2005
    BibTeX Link
    @InProceedings{HU_2005_Huber_g,
      Title                    = {{Fourier domain mode locked lasers for OCT imaging at up to 290 kHz sweep rates}},
      Author                   = {Huber, R and Taira, K and Wojtkowski, M and Fujimoto, J G},
      Booktitle                = {European Conference on Biomedical Optics 2005},
      Year                     = {2005},
      Editor                   = {Drexler, Wolfgang},
      Month                    = jun,
      Pages                    = {58611B--58611B--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.641732},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=867251}
    }
  • Huber, Robert A and Taira, Kenji and Fujimoto, James G: Fourier Domain Mode Locking: Overcoming limitations of frequency swept light sources and pulsed lasers. in Conference on Lasers and Electro-Optics Europe/ European Quantum Electronics Conference (CLEO/Europe - EQEC 2005), 2005
    BibTeX
    @InProceedings{HU_2005_Huber_a,
      Title                    = {{Fourier Domain Mode Locking: Overcoming limitations of frequency swept light sources and pulsed lasers}},
      Author                   = {Huber, Robert A and Taira, Kenji and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics Europe/ European Quantum Electronics Conference (CLEO/Europe - EQEC 2005)},
      Year                     = {2005}
    }
  • Huber, Robert and Taira, Kenji and Wojtkowski, Maciej and Ko, Tony H and Fujimoto, James G and Hsu, Kevin: High-speed frequency swept light source for Fourier domain OCT at 20 kHz A-scan rate. in Biomedical Optics 2005, pp. 96-100, International Society for Optics and Photonics, apr,, 2005
    BibTeX Link
    @InProceedings{HU_2005_Huber_f,
      Title                    = {{High-speed frequency swept light source for Fourier domain OCT at 20 kHz A-scan rate}},
      Author                   = {Huber, Robert and Taira, Kenji and Wojtkowski, Maciej and Ko, Tony H and Fujimoto, James G and Hsu, Kevin},
      Booktitle                = {Biomedical Optics 2005},
      Year                     = {2005},
      Editor                   = {Tuchin, Valery V and Izatt, Joseph A and Fujimoto, James G},
      Month                    = apr,
      Pages                    = {96--100},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.592552},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1323562}
    }
  • Huber, Robert and Taira, Kenji and Ko, Tony H and Wojtkowski, Maciej and Srinivasan, Vivek and Hsu, Kevin and Fujimoto, James G: High-Speed, Amplified, Frequency Swept Laser at 20 kHz Sweep Rates for OCT Imaging - Technical Digest (CD). in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, pp. JThE33, Optical Society of America, 2005
    BibTeX
    @InProceedings{HU_2005_Huber_b,
      Title                    = {{High-Speed, Amplified, Frequency Swept Laser at 20 kHz Sweep Rates for OCT Imaging - Technical Digest (CD)}},
      Author                   = {Huber, Robert and Taira, Kenji and Ko, Tony H and Wojtkowski, Maciej and Srinivasan, Vivek and Hsu, Kevin and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies},
      Year                     = {2005},
      Pages                    = {JThE33},
      Publisher                = {Optical Society of America},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=QELS-2005-JThE33}
    }
  • Huber, Robert and Köhler, Thomas and Lenz, Martin O and Bamberg, Ernst and Kalmbach, Rolf and Engelhard, Martin and Wachtveitl, Josef: pH-dependent photoisomerization of retinal in proteorhodopsin.. Biochemistry, no. 44, pp. 1800-6, feb,, 2005
    BibTeX Link
    @Article{HU_2005_Huber_e,
      Title                    = {{pH-dependent photoisomerization of retinal in proteorhodopsin.}},
      Author                   = {Huber, Robert and K\"{o}hler, Thomas and Lenz, Martin O and Bamberg, Ernst and Kalmbach, Rolf and Engelhard, Martin and Wachtveitl, Josef},
      Journal                  = {Biochemistry},
      Year                     = {2005},
    
      Month                    = feb,
      Number                   = {6},
      Pages                    = {1800--6},
      Volume                   = {44},
    
      Doi                      = {10.1021/bi048318h},
      ISSN                     = {0006-2960},
      Url                      = {http://pubs.acs.org/doi/abs/10.1021/bi048318h}
    }
    
  • Sharma, Vikas and Kowalevicz Jr., Andrew M and Huber, Robert and Fujimoto, James G and Minoshima, Kaoru: Three Dimensional Waveguide Splitters Fabricated in Glass Using a Femtosecond Laser Oscillator - Technical Digest (CD). in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, pp. CThCC4, Optical Society of America, 2005
    BibTeX Link
    @InProceedings{HU_2005_Sharma_a,
      Title                    = {{Three Dimensional Waveguide Splitters Fabricated in Glass Using a Femtosecond Laser Oscillator - Technical Digest (CD)}},
      Author                   = {Sharma, Vikas and {Kowalevicz Jr.}, Andrew M and Huber, Robert and Fujimoto, James G and Minoshima, Kaoru},
      Booktitle                = {Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies},
      Year                     = {2005},
      Pages                    = {CThCC4},
      Publisher                = {Optical Society of America},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO-2005-CThCC4}
    }
  • Huber, R and Wojtkowski, M and Fujimoto, James G and Jiang, J Y and Cable, A E: Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm. Optics Express, no. 13, pp. 10523 - 10538, 2005
    BibTeX Link
    @Article{HU_2005_Huber_d,
      Title                    = {{Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm}},
      Author                   = {Huber, R and Wojtkowski, M and Fujimoto, James G and Jiang, J Y and Cable, A E},
      Journal                  = {Optics Express},
      Year                     = {2005},
      Number                   = {26},
      Pages                    = {10523 -- 10538 },
      Volume                   = {13},
    
      Doi                      = {10.1364/OPEX.13.010523},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-26-10523}
    }
  • Ahlers, C. and Michels, S. and Elsner, H. and Birngruber, R. and Pruente, C. and Schmidt-Erfurth, U.: Topographic angiography and optical coherence tomography: A correlation of imaging characteristics. European Journal of Ophthalmology, no. 15, pp. 774-781, 2005
    BibTeX Link
    @article{Ahlers2005,
       author = {Ahlers, C. and Michels, S. and Elsner, H. and Birngruber, R. and Pruente, C. and Schmidt-Erfurth, U.},
       title = {Topographic angiography and optical coherence tomography: A correlation of imaging characteristics},
       journal = {European Journal of Ophthalmology},
       volume = {15},
       number = {6},
       pages = {774-781},
       note = {990KX
    Times Cited:8
    Cited References Count:8},
       abstract = {PURPOSE. Topographic angiography (TAG) using confocal scanning laser angiography and optical coherence tomography (OCT) are new imaging modalities that have been introduced during recent years. OCT and TAG imaging were compared to specify the characteristics of each imaging modality.
    METHODS. TAG using fluorescein angiography (FA) provides a three-dimensional pro file of the vascular structures based on the analysis of a set of 32 confocal images over a depth of 4 mm. OCT provides cross-sectional images of the neurosensory retina and the retinal pigment epithelium-choriocapillary complex (RPE-CC). The authors compared and evaluated both modalities in 10 patients with predominantly classic choroidal neovascularization (CNV), 10 patients with serous pigment epithelial detachment (PED), and 10 patients with geographic RPE atrophy, all secondary to age-related macular degeneration (ARMD).
    RESULTS. In patients with classic CNV, TAG detected neovascular structures and delineated their configuration. In PEDs pooling of extravascular fluid is demonstrated, and in geographic RPE atrophy TAG showed reduced choroidal perfusion. Classic CNV was demonstrated by OCT as a hyperreflective band at the level of the RPE-CC, and PED showed a dome-shaped RPE detachment. In geographic RPE atrophy, OCT imaged loss of the RPE band and had an increased depth resolution.
    CONCLUSIONS. TAG and OCT are useful imaging modalities in the evaluation of ARMD cases. TAG visualizes the vascular configuration and dynamic perfusion and leakage changes. OCT is able to document intra-, subretinal, and sub-RPE fluid accumulation secondary to CNV Both modalities may provide further valuable insight into ARMD pathogenesis, enhance diagnostic quality, and improve the assessment of therapeutic effects.},
       keywords = {age-related macular degeneration
    choroidal neovascularization
    geographic atrophy
    imaging
    optical coherence tomography
    pigment epithelial detachment
    topographic angiography
    macular degeneration
    choroidal neovascularization
    disease},
       ISSN = {1120-6721},
       url = {<Go to ISI>://WOS:000233742900019},
       year = {2005},
       type = {Journal Article}
    }
    

2004

  • Schüle, G. and Framme, C. and Roider, J. and Brinkmann, R: Noninvasive optoacoustic temperature determination at the fundus of the eye during laser irradiation. Journal of Biomedical Optics, no. 9, pp. 173-179, 2004
    BibTeX
    @article{Schüle,
       author = {Schüle, G. and Framme, C. and Roider, J. and Brinkmann, R},
       title = {Noninvasive optoacoustic temperature determination at the fundus of the eye during laser irradiation},
       journal = {Journal of Biomedical Optics},
       volume = {9},
       number = {1},
       pages = {173-179},
       year = {2004}
    }
    
  • Roegener, J and Brinkmann, R and Lin, C P: Pump-probe detection of laser-induced microbubble formation in retinal pigment epithelium cells. J Biomedical Optics, no. 9, pp. 367-371, 2004
    BibTeX
    @article{Roegener,
       author = {Roegener, J and Brinkmann, R and Lin, C P},
       title = {Pump-probe detection of laser-induced microbubble formation in retinal pigment epithelium cells},
       journal = {J Biomedical Optics},
       volume = {9},
       number = {2},
       pages = {367-371},
       year = {2004}
    }
    
  • Mori, H and Hayashi, Y: Repair of leaking blebs using conunctiva and adjacent half-thickness cornea as free autografts.. Japanese Journal of Clinical Ophthalmology, no. 58, pp. 1695-1698, 2004
    BibTeX
    @article{Hayashi,
       author = {Mori, H and Hayashi, Y},
       title = {Repair of leaking blebs using conunctiva and adjacent half-thickness cornea as free autografts.},
       journal = {Japanese Journal of Clinical Ophthalmology},
       volume = {58},
       number = {9},
       pages = {1695-1698},
       year = {2004}
    }
    
  • Kracht, D. and Brinkmann, R: Green Q-switched microsecond laser pulses by overcoulped intracavity second harmonic generation. no. 231, pp. 319-324, 2004
    BibTeX
    @misc{Kracht,
       author = {Kracht, D. and Brinkmann, R},
       title = {Green Q-switched microsecond laser pulses by overcoulped intracavity second harmonic generation},
       volume = {231},
       pages = {319-324},
       year = {2004}
    }
    
  • Löning, M. and Diddens, H. and W, Küpker and Diedrich, K. and Hüttmann, G.: Laparoscopic Fluorescence Detection of Ovarian Carcinoma Metastases Using 5-Aminolevulinic Acid-Induced Protoporphyrin IX. no. 100, 2004
    BibTeX
    @article{Löning,
       author = {Löning, M. and Diddens, H. and W, Küpker and Diedrich, K. and Hüttmann, G.},
       title = {Laparoscopic Fluorescence Detection of Ovarian Carcinoma Metastases Using 5-Aminolevulinic Acid-Induced Protoporphyrin IX},
       journal = {CANCER},
       volume = {100},
       number = {8},
       year = {2004}
    }
    
    
  • Rau, K.R. and Vogel, A. and Venugopalan, V.: Investigation of laser-induced cell lysis using time-resolved imaging. Appl Phys Lett, no. 84, pp. 2940-2942, 2004
    BibTeX
    @article{Rau,
       author = {Rau, K.R. and Vogel, A. and Venugopalan, V.},
       title = {Investigation of laser-induced cell lysis using time-resolved imaging},
       journal = {Appl Phys Lett},
       volume = {84},
       number = {15},
       pages = {2940-2942},
       year = {2004}
    }
    
  • Porksen, E. and Elsner, H. and Theisen-Kunde, D. and Brinkmann, R. and Klatt, C. and Bunse, A. and Hamilton, P. and Birngruber, R. and Lagua, H. and Roider, J.: Clinical application of optoacoustic on-line dosimetry in Selective-RPE-laser-treatment (SRT). Investigative Ophthalmology & Visual Science, no. 45, pp. U363-U363, 2004
    BibTeX Link
    @article{Porksen2004,
       author = {Porksen, E. and Elsner, H. and Theisen-Kunde, D. and Brinkmann, R. and Klatt, C. and Bunse, A. and Hamilton, P. and Birngruber, R. and Lagua, H. and Roider, J.},
       title = {Clinical application of optoacoustic on-line dosimetry in Selective-RPE-laser-treatment (SRT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {45},
       pages = {U363-U363},
       note = {Suppl. 2
    846TC
    4074
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000223338201270},
       year = {2004},
       type = {Journal Article}
    }
    

  • BibTeX
    article{Elsner2004,
       author = {Elsner, H. G. I. and Poerksen, E. and Klatt, C. and Bunse, A. and Theisen-Kunde, D. and Brinkmann, R. and Hamilton, P. and Birngruber, R. and Laqua, H. and Roider, J.},
       title = {Functional and angiographic findings in patients with diabetic maculopathy treated with Selective-RPE-laser-treatment (SRT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {45},
       pages = {U368-U368},
       note = {Suppl. 2
    846TC
    4105
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000223338201298},
       year = {2004},
       type = {Journal Article}
    }
    
  • Framme, C. and Alt, C. and Schnell, S. and Brinkmann, R. and Lin, C. P.: Selektive RPE-Behandlung (SRT) durch Laserscanning eines CW- Lasers. in 102nd Congress Deutsche Ophthalmologische Gesellschaft,
    BibTeX
    @inproceedings{Framme,
       author = {Framme, C. and Alt, C. and Schnell, S. and Brinkmann, R. and Lin, C. P.},
       title = {Selektive RPE-Behandlung (SRT) durch Laserscanning eines CW- Lasers},
       booktitle = {102nd Congress Deutsche Ophthalmologische Gesellschaft},
    
    }
    
  • Framme, C. and Schuele, G. and Roider, J. and Birngruber, R. and Brinkmann, R.: Influence of pulse duration and pulse number in selective RPE laser treatment. Lasers Surg Med, no. 34, pp. 206-15, 2004
    BibTeX
    @article{Framme,
       author = {Framme, C. and Schuele, G. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Influence of pulse duration and pulse number in selective RPE laser treatment},
       journal = {Lasers Surg Med},
       volume = {34},
       number = {3},
       pages = {206-15},
       note = {0196-8092 (Print)
    Journal Article
    Research Support, Non-U.S. Gov't},
       abstract = {BACKGROUND AND OBJECTIVES: The therapeutic effect of laser treatment for macular diseases is related to the damage to the retinal pigment epithelium (RPE) and the subsequent restoration of the defect due to RPE proliferation. In contrast to conventional laser treatment, it is possible to damage the RPE selectively and to spare the photoreceptors by using repetitive microsecond laser pulses. It was the aim of the study to investigate the influence of pulse duration and number of pulses on angiographically and ophthalmoscopically visible retinal damage thresholds in order to optimize treatment modalities. STUDY DESIGN/MATERIALS AND METHODS: In total, 625 laser lesions with various parameters were applied to the retina in 11 eyes of 6 Chinchilla breed rabbits using an experimental laser system (Nd:YLF at 527 nm). Pulse duration (1.7 microseconds and 200 nanoseconds) and number of pulses (100, 10, and 1 pulses) were varied at a constant repetition rate of 100 Hz. Damage thresholds were determined in terms of ophthalmoscopic and fluorescein angiographic visibility, and the therapeutic window (TW; angiographic ED(50) vs. ophthalmoscopic ED(50)) as well as the safety range (SR; angiographic ED(84) vs. ophthalmoscopic ED(16)) between both thresholds were calculated. Selected laser lesions were evaluated by histology. RESULTS: Generally, the ED(50) radiant exposure for angiographic visibility decreases for shorter laser pulses and with an increase in the number of pulses. The TW for both pulse durations (1.7 microseconds and 200 nanoseconds) was wider with 100 pulses than with single pulses. The widest TW was found for 100 pulses at 200 nanoseconds pulse duration (5.9-fold above the angiographic threshold), and the smallest TW with a factor of 1.6 was found for 1.7 microseconds single pulses. In terms of SR, only irradiation with 100 pulses at 200 nanoseconds pulse duration was associated with a ratio >2. Independently of pulse duration, histological examination of laser sites 1 hour after irradiation revealed widely intact photoreceptors, while the underlying RPE was damaged. CONCLUSIONS: Pulse duration and number of pulses have a significant influence on RPE damage thresholds and consecutively on TW and SR. Because fundus pigmentation in humans may vary intra- and interindividually by a factor of 2, a large TW and ideally also a large SR should be ensured in a clinical treatment context. In rabbits, the safety range with 200 nanoseconds pulses is higher than with the pulse duration of 1.7 microseconds currently in clinical use. These findings suggest the need for clinical pilot studies to prove whether these results can be transposed to the situation in humans.},
       keywords = {Animals
    *Laser Coagulation/adverse effects
    Pigment Epithelium of Eye/*injuries
    Rabbits
    Time Factors},
       year = {2004}
    }
  • Framme, C. and Schule, G. and Roider, J. and Birngruber, R. and Brinkmann, R.: Online autofluorescence measurements during selective RPE laser treatment. no. 242, pp. 863-9, Oct, 2004
    BibTeX
    @misc{Framme,
       author = {Framme, C. and Schule, G. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Online autofluorescence measurements during selective RPE laser treatment},
       volume = {242},
       number = {10},
       pages = {863-9},
       month = {Oct},
       note = {0721-832X (Print)
    Journal Article},
       abstract = {BACKGROUND: Fundus autofluorescence (AF) is derived from the lipofuscin contained by the retinal pigment epithelial cells. Using a scanning laser ophthalmoscope, two-dimensional AF measurements of the ocular fundus can be achieved. Directly after conventional photocoagulation and also after selective RPE laser treatment (SRT) with ophthalmoscopically non-visible laser lesions, irradiated areas reveal reduced AF, indicating RPE damage. Since the green treatment laser beam could also be used for AF excitation, the aim of this study was to evaluate whether absolute measurements of AF can be performed, and also possible changes in AF detected, online during SRT. METHODS: SRT was carried out by use of a frequency-doubled Nd:YLF laser (wavelength 527 nm, pulse duration 1.7 micros, repetition rate 500 and 100 Hz, number of pulses 100 and 30, single pulse energy 50-130 microJ) in vitro (porcine RPE; retinal spot size 160 microm) and during patient treatment (retinal spot size 176 microm). During irradiation, fluorescence light from the RPE was decoupled from the laser light inside the slit lamp and detected by a photomultiplier or photodiode at wavelengths above 550 nm. Additionally, temperature-dependent fluorescence intensity measurements of A2-E, the main fluorescent component of lipofuscin, were performed in a different in-vitro setup. RESULTS: The intensity of AF decreased over the number of applied pulses during laser irradiation, and this trend was more pronounced in porcine RPE samples than during human treatment. In vitro, the AF intensity decreased by about 22%; however, only a weak signal was detected. When treating patients, the AF intensity was strong and the rate of decay of fluorescence intensity with number of pulses was greater when irradiating at 500 Hz than at the 100 Hz repetition rate. However, for both repetition rates the AF decay was merely up to 6-8% over the number of pulses per laser spot. Fluorescence intensity of A2-E decreased linearly with increasing temperature at about 1% per 1 degrees C and was completely reversible. CONCLUSIONS: Online measurements of AF during selective RPE laser treatment are possible and reveal a decay in AF as a function of the number of laser pulses applied to the RPE. If A2-E results can be transferred to RPE fluorescence, the AF decay could be related to the temperature increase within the tissue during treatment. Further clinical studies-in SRT as well as in conventional laser photocoagulation-might be able to show online AF changes on different areas of the retina and on different pathologies. Due to the temperature dependence of the fluorescence, on-line AF measurements during laser treatments such as photocoagulation or TTT may be able to be used as a real-time method for temperature monitoring.},
       keywords = {Animals
    Diabetic Retinopathy/*surgery
    *Fluorescence
    Humans
    *Laser Coagulation
    Lasers/diagnostic use
    Lipofuscin/*metabolism
    Ophthalmoscopy/methods
    Pigment Epithelium of Eye/*metabolism/surgery
    Swine},
       year = {2004}
    }
  • Framme, C and Schüle, G and Birngruber, R and Roider, J and Schütt, F and Kopitz, J and Holz, F and Brinkmann, R: Temperature dependent fluorescence of A2-E, the main fluorescent lipofuscin component in the RPE. no. 29, pp. 287-291, 2004
    BibTeX
    @article{Framme,
       author = {Framme, C and Schüle, G and Birngruber, R and Roider, J and Schütt, F and Kopitz, J and Holz, F and Brinkmann, R},
       title = {Temperature dependent fluorescence of A2-E, the main fluorescent lipofuscin component in the RPE},
       journal = {Curr Eye Res},
       volume = {29},
       number = {4-5},
       pages = {287-291},
       year = {2004}
    }
  • Brendel, T and Brinkmann, R: Mid-IR laser induced superheating of water and its quantification by an optical temperature probe. Appl Optics, no. 43, pp. 1856-1862, 2004
    BibTeX
    @article{Brendel,
       author = {Brendel, T and Brinkmann, R},
       title = {Mid-IR laser induced superheating of water and its quantification by an optical temperature probe},
       journal = {Appl Optics},
       volume = {43},
       number = {9},
       pages = {1856-1862},
       year = {2004}
    }
  • Lankenau, E. and Schumacher, M. and Koch, P. and König, F. and Daniltchenko, D. and Schmorr, D. and Hüttmann, G.: Dispersion compensation for proximal scanning rigid OCT endoscopes. in SPIE Proc., no. 5316, pp. 172-177,
    BibTeX
    @inproceedings{Lankenau,
       author = {Lankenau, E. and Schumacher, M. and Koch, P. and König, F. and Daniltchenko, D. and Schmorr, D. and Hüttmann, G.},
       title = {Dispersion compensation for proximal scanning rigid OCT endoscopes},
       booktitle = {SPIE Proc.},
       volume = {5316},
       pages = {172-177},
    
    }
  • Daniltchenko, D. and Lankenau, E. and König, F. and Shay, B. and Hüttmann, G. and Sachs, M.D. and Schnorr, D.: In Vivo Optical Coherence Tomography in Endoscopic Diagnosis of Bladder Disease. in Clinical technoligies and systems, no. 5316, pp. 473-478, Proc Soc Photo-Opt Instrum Eng, SPIE 2004, 2004
    BibTeX
    @inbook{Daniltchenko,
       author = {Daniltchenko, D. and Lankenau, E. and König, F. and Shay, B. and Hüttmann, G. and Sachs, M.D. and Schnorr, D.},
       title = {In Vivo Optical Coherence Tomography in Endoscopic Diagnosis of Bladder Disease},
       booktitle = {Clinical technoligies and systems},
       editor = {Vo-Dinh, Tuan},
       publisher = {Proc Soc Photo-Opt Instrum Eng, SPIE 2004},
       volume = {5316},
       pages = {473-478},
       year = {2004}
    }
  • Klatt, C. and Bunse, A. and Elsner, H. and Poerksen, E. and Theisen, D. and Brinkman, R. and Hamilton, P. and Birngruber, R. and Laqua, H. and Roider, J.: OCT-findings in patients treated with Selective-RPE-laser-treatment (SRT). Investigative Ophthalmology & Visual Science, no. 45, pp. U366-U366, 2004
    BibTeX Link
    @article{Klatt2004,
       author = {Klatt, C. and Bunse, A. and Elsner, H. and Poerksen, E. and Theisen, D. and Brinkman, R. and Hamilton, P. and Birngruber, R. and Laqua, H. and Roider, J.},
       title = {OCT-findings in patients treated with Selective-RPE-laser-treatment (SRT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {45},
       pages = {U366-U366},
       note = {Suppl. 2
    846TC
    4091
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000223338201285},
       year = {2004},
       type = {Journal Article}
    }
    
  • Kirsch, C. and Schneider-Brachert, W. and Grossmann, D. and Ebert, S. and Schlichting, S. and Birngruber, R. and Morgner, A. and Madisch, A. and Stolte, M. and Lehn, N. and Miehlke, S.: The efficacy of omeprazole/esomeprazole-based first-line and rescue therapies for treatment of Helicobacter pylori infection is not affected by polymorphisms of cytochrome P450-2C19. Gastroenterology, no. 126, pp. A189-A189, 2004
    BibTeX Link
    @article{Kirsch2004,
       author = {Kirsch, C. and Schneider-Brachert, W. and Grossmann, D. and Ebert, S. and Schlichting, S. and Birngruber, R. and Morgner, A. and Madisch, A. and Stolte, M. and Lehn, N. and Miehlke, S.},
       title = {The efficacy of omeprazole/esomeprazole-based first-line and rescue therapies for treatment of Helicobacter pylori infection is not affected by polymorphisms of cytochrome P450-2C19},
       journal = {Gastroenterology},
       volume = {126},
       number = {4},
       pages = {A189-A189},
       note = {Suppl. 2
    813EK
    Times Cited:0
    Cited References Count:0},
       ISSN = {0016-5085},
       url = {<Go to ISI>://WOS:000220890200944},
       year = {2004},
       type = {Journal Article}
    }
    
  • Framme, C. and Flucke, B. and Birngruber, R.: Comparison of reduced and standard light application in photodynamic treatment in two rabbit models. Investigative Ophthalmology & Visual Science, no. 45, pp. U81-U81, 2004
    BibTeX Link
    @article{Framme2004,
       author = {Framme, C. and Flucke, B. and Birngruber, R.},
       title = {Comparison of reduced and standard light application in photodynamic treatment in two rabbit models},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {45},
       pages = {U81-U81},
       note = {Suppl. 2
    846TC
    3189
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000223338200390},
       year = {2004},
       type = {Journal Article}
    }
    
  • Roach, W. P. and Cain, C. P. and Narayan, D. G. and Noojin, G. D. and Boppart, S. A. and Birngruber, R. and Fujimoto, J. G. and Toth, C. A.: Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size. Journal of Biomedical Optics, no. 9, pp. 1288-1296, 2004
    BibTeX Link
    @article{Roach2004,
       author = {Roach, W. P. and Cain, C. P. and Narayan, D. G. and Noojin, G. D. and Boppart, S. A. and Birngruber, R. and Fujimoto, J. G. and Toth, C. A.},
       title = {Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size},
       journal = {Journal of Biomedical Optics},
       volume = {9},
       number = {6},
       pages = {1288-1296},
       note = {879DU
    Times Cited:2
    Cited References Count:19},
       abstract = {We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induced breakdown (LIB) at the site of laser delivery. Single 3-ps pulses of 580-nm laser energy are delivered over a range of spot sizes to the retina of Macaca mulatta. The retinal response is captured sequentially with optical coherence tomography (OCT). The in vivo OCT images and the extent of pathology on final microscopic sections of the laser site are compared. With delivery of a laser pulse with peak irradiance greater than that required for LIB, OCT and light micrographs demonstrate inner retinal injury with many intraretinal and/or vitreous hemorrhages. In contrast, broad outer retinal injury with minimal to no choriocapillaris effect is seen after delivery of laser pulses to a larger retinal area (60 to 300 mum diam) when peak irradiance is less than that required for LIB. The broader lesions extend into the inner retina when higher energy delivery produces intraretinal injury. Microscopic examination of stained fixed tissues provide better resolution of retinal morphology than OCT. OCT provides less resolution but could be guided over an in vivo, visible retinal lesion for repeated sampling over time during the evolution of the lesion formation. For 3-ps visible wavelength laser pulses, varying the spot size and laser energy directly affects the extent of retinal injury. This again is believed to be partly due to the onset of LIB, as seen in previous studies. Spot-size dependence should be considered when comparing studies of retinal effects or when pursuing a specific retinal effect from ultrashort laser pulses. (C) 2004 Society of Photo-Optical Instrumentation Engineers.},
       keywords = {histopathology
    laser
    laser-tissue interaction
    ophthalmology
    optical coherence tomography
    retina
    ultrashort laser pulses
    wound healing
    induced breakdown thresholds
    optical coherence tomography
    aqueous-media
    1st-order model
    lesions
    damage
    computation
    injury},
       ISSN = {1083-3668},
       DOI = {Doi 10.1117/1.1805554},
       url = {<Go to ISI>://WOS:000225697400023},
       year = {2004},
       type = {Journal Article}
    }
    
  • Koch, Peter and Huettmann, Gereon and Schleiermacher, Hansfrieder and Eicholz, Joerg and Koch, Edmund: Linear OCT system with down conversion of the fringe pattern. no. 5316, pp. 260-267, SPIE,
    BibTeX
    @inproceedings{Koch,
       author = {Koch, Peter and Huettmann, Gereon and Schleiermacher, Hansfrieder and Eicholz, Joerg and Koch, Edmund},
       title = {Linear OCT system with down conversion of the fringe pattern},
       editor = {Valery, V. Tuchin and Joseph, A. Izatt and James, G. Fujimoto},
       publisher = {SPIE},
       volume = {5316},
       pages = {260-267},
    
    }
  • Ahlers, C. and Michels, S. and Birngruber, R. and Schmidt-Erfurth, U.: Differentiation of lesion types in exsudative age-related macular degeneration using current imaging methods. Investigative Ophthalmology & Visual Science, no. 45, pp. U61-U61, 2004
    BibTeX Link
    @article{Ahlers2004,
       author = {Ahlers, C. and Michels, S. and Birngruber, R. and Schmidt-Erfurth, U.},
       title = {Differentiation of lesion types in exsudative age-related macular degeneration using current imaging methods},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {45},
       pages = {U61-U61},
       note = {Suppl. 2
    846TC
    3071
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000223338200273},
       year = {2004},
       type = {Journal Article}
    }
    
  • Helbing, Jan and Bregy, Harald and Bredenbeck, Jens and Pfister, Rolf and Hamm, Peter and Huber, Robert and Wachtveitl, Josef and De Vico, Luca and Olivucci, Massimo: A fast photoswitch for minimally perturbed peptides: investigation of the trans->cis photoisomerization of N-methylthioacetamide.. Journal of the American Chemical Society, no. 126, pp. 8823-34, jul,, 2004
    BibTeX Link
    @Article{HU_2004_Helbing_a,
      Title                    = {{A fast photoswitch for minimally perturbed peptides: investigation of the trans-->cis photoisomerization of N-methylthioacetamide.}},
      Author                   = {Helbing, Jan and Bregy, Harald and Bredenbeck, Jens and Pfister, Rolf and Hamm, Peter and Huber, Robert and Wachtveitl, Josef and {De Vico}, Luca and Olivucci, Massimo},
      Journal                  = {Journal of the American Chemical Society},
      Year                     = {2004},
    
      Month                    = jul,
      Number                   = {28},
      Pages                    = {8823--34},
      Volume                   = {126},
    
      Doi                      = {10.1021/ja049227a},
      ISSN                     = {0002-7863},
      Url                      = {http://pubs.acs.org/doi/abs/10.1021/ja049227a}
    }

2003

  • Vogel, A. and Venugopalan, V.: Mechanisms of Pulsed Laser Ablation of Biological Tissues. Chem. Rev., no. 103, pp. 577-644, 2003
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Venugopalan, V.},
       title = {Mechanisms of Pulsed Laser Ablation of Biological Tissues},
       journal = {Chem. Rev.},
       volume = {103},
       pages = {577-644},
       year = {2003}
    }
    
  • Huettmann, Gereon and Radt, Benno and Serbin, Jesper and Birngruber, Reginald: Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses. no. 5142, pp. 88-95, SPIE,
    BibTeX
    @inproceedings{Hüttmann2003,
       author = {Huettmann, Gereon and Radt, Benno and Serbin, Jesper and Birngruber, Reginald},
       title = {Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses},
       editor = {Rudolf, W. Steiner},
       publisher = {SPIE},
       volume = {5142},
       pages = {88-95},
    }
    
    
    
    
  • Vogel, Alfred: Mechanism of short-pulsed plasma-mediated laser ablation and disruption. in Lasers in Ophthalmology - Basic, Diagnostic and Surgical Aspects, pp. pp. 99-113, Kugler Publications, The Hague, The Netherlands, 2003
    BibTeX
    @inbook{Vogel,
       author = {Vogel, Alfred},
       title = {Mechanism of short-pulsed plasma-mediated laser ablation and disruption},
       booktitle = {Lasers in Ophthalmology - Basic, Diagnostic and Surgical Aspects},
       editor = {Frankenhauser, F. and Kwasniewska, S.},
       publisher = {Kugler Publications, The Hague, The Netherlands},
       pages = {pp. 99-113},
       year = {2003}
    }
    
  • Vogel, A. and Venugopalan, V.: Additions and Corrections. Chemical Reviews, no. 103, pp. 2079, 2003
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Venugopalan, V.},
       title = {Additions and Corrections},
       journal = {Chemical Reviews},
       volume = {103},
       number = {5},
       pages = {2079},
       year = {2003}
    }
    
  • Löning, M. and Lankenau, E. and Strunck, C. and Krokowski, M. and Hillbricht, S. and Diedrich, K. and Hüttmann, G.: Optische Kohärenztomographie - ein neues hochauflösendes Schnittbildverfahren als Ergänzung zur Kolposkopie. Geburtsh Frauenheilk, no. 63, pp. 1158-1161, 2003
    BibTeX
    @article{Löning,
       author = {Löning, M. and Lankenau, E. and Strunck, C. and Krokowski, M. and Hillbricht, S. and Diedrich, K. and Hüttmann, G.},
       title = {Optische Kohärenztomographie - ein neues hochauflösendes Schnittbildverfahren als Ergänzung zur Kolposkopie},
       journal = {Geburtsh Frauenheilk},
       volume = {63},
       pages = {1158-1161},
       year = {2003}
    }
    
  • Miura, Y. and Yanagihara, N. and Imamura, H. and Kaida, M. and Moriwaki, M. and Shiraki, K. and Miki, T.: Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro. Jpn J Ophthalmol, no. 47, pp. 268-75, 2003
    BibTeX
    @article{Miura2003,
       author = {Miura, Y. and Yanagihara, N. and Imamura, H. and Kaida, M. and Moriwaki, M. and Shiraki, K. and Miki, T.},
       title = {Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro},
       journal = {Jpn J Ophthalmol},
       volume = {47},
       number = {3},
       pages = {268-75},
       note = {Miura, Yoko
    Yanagihara, Nobuyo
    Imamura, Hitoshi
    Kaida, Mayumi
    Moriwaki, Mitsuyasu
    Shiraki, Kunihiko
    Miki, Tokuhiko
    eng
    Research Support, Non-U.S. Gov't
    2003/06/05 05:00
    Jpn J Ophthalmol. 2003 May-Jun;47(3):268-75.},
       abstract = {PURPOSE: A defect in retinal pigment epithelial (RPE) cells may cause dysfunction of the neural retina, so rapid recovery of differentiated RPE cells is required after RPE injury. We investigated the effect of hepatocyte growth factor (HGF) on wound healing in RPE cells. METHODS: Confluent monolayers of bovine RPE cells were denuded, and the cells were allowed to recover in the presence or absence of HGF. The effect of HGF on RPE cell proliferation was evaluated by a 3-(4;5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tet raz olium assay. In a migration assay, mitomycin C was used to inhibit proliferation, and the number of migrated cells was counted. The signaling pathways involved were examined using inhibitors of mitogen-activated protein kinase (MAPK), phosphatidylinositol-3 (PI3) kinase and protein kinase C pathways. RESULTS: At 80 ng/mL, HGF stimulated the wound closure of RPE monolayers and rendered the restituted cells more epithelioid in shape. HGF at 10 ng/mL stimulated RPE cell migration the most, whereas 80 ng/mL of HGF inhibited migration, but stimulated proliferation the most. In particular, PI3 kinase and MAPK inhibitor inhibited PRE cell migration and proliferation, respectively. CONCLUSIONS: HGF stimulated wound closure in cultured RPE cells, and rendered restituted cells epithelioid in shape. HGF may become a therapeutic candidate for RPE wound healing.},
       keywords = {Animals
    Cattle
    Cell Count
    Cell Division/*drug effects
    Cell Movement/*drug effects
    Cells, Cultured
    Enzyme Inhibitors/pharmacology
    Hepatocyte Growth Factor/*pharmacology
    Mitogen-Activated Protein Kinases/antagonists & inhibitors
    Phosphatidylinositol 3-Kinases/antagonists & inhibitors
    Pigment Epithelium of Eye/*cytology/physiology
    Protein Kinase C/antagonists & inhibitors
    Signal Transduction
    Wound Healing/*drug effects},
       year = {2003}
    }
    
  • Niemeyer, M. and Michels, S. and Birngruber, R. and Schmidt-Erfurth, U.: Three-dimensional imaging of photodynamic effects in choroidal neovascularization and collateral choroid. Investigative Ophthalmology & Visual Science, no. 44, pp. U297-U297, 2003
    BibTeX Link
    @article{Niemeyer2003,
       author = {Niemeyer, M. and Michels, S. and Birngruber, R. and Schmidt-Erfurth, U.},
       title = {Three-dimensional imaging of photodynamic effects in choroidal neovascularization and collateral choroid},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {44},
       pages = {U297-U297},
       note = {Suppl. 1
    709CH
    1098
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000184606801048},
       year = {2003},
       type = {Journal Article}
    }
    
  • Hoerauf, H. and Brix, A. and Scholz, C. and Winkler, J. and Droge, G. and Birngruber, R. and Vogel, A. and Laqua, H.: [Retinal photoablation with the Erbium:YAG laser. Initial experimental results for traction-free removal of tissue]. Ophthalmologe, no. 100, pp. 115-21, Feb,
    BibTeX
    @article{Hoerauf,
       author = {Hoerauf, H. and Brix, A. and Scholz, C. and Winkler, J. and Droge, G. and Birngruber, R. and Vogel, A. and Laqua, H.},
       title = {[Retinal photoablation with the Erbium:YAG laser. Initial experimental results for traction-free removal of tissue]},
       journal = {Ophthalmologe},
       volume = {100},
       number = {2},
       pages = {115-21},
       note = {0941-293X (Print)
    Comparative Study
    English Abstract
    Journal Article},
       month = {Feb},
       abstract = {BACKGROUND: To investigate the potential of an Er:YAG laser for precise and traction-free removal of retinal layers in vitro. MATERIAL AND METHODS: Retinal ablation in porcine retinal explants was performed using a free running Er:YAG laser focused either into a low-OH quartz fiber or a sapphire fiber. The explants were treated under air or perfluorodecaline (PFD). The ablation depth was evaluated by optical coherence tomography (OCT) and histology sections. RESULTS: A radiant exposure of 5.0 J/cm(2) under air and PFD resulted in complete transsection of the neurosensory retina.Between 3.5 and 2.0 J/cm(2) the ablation depth and the defect patterns varied markedly and adjacent thermal zones areas were seen. Below 2.0 J/cm(2) no defects could be created in air, whereas under PFD the ablation extended into the ganglion cell layer. Ablations using a sapphire fiber and 2.0 J/cm(2) showed a significantly higher reproducibility of ablation depth, and homogeneous defect patterns limited to the nerve fiber layer could be produced without thermal damage. CONCLUSIONS: The Er:YAG laser system with a low-OH quartz fiber allowed ablation of inner retinal layers in vitro, but revealed a variable ablation depth and low reproducibility.However, a sapphire fiber showed markedly improved results. Therefore its use during vitreoretinal surgery seems possible not only as a cutting device but also as a tool for the ablation of fine retinal structures.},
       keywords = {Animals
    Erbium
    Fluorocarbons
    Laser Surgery/*methods
    *Ophthalmologic Surgical Procedures
    Retina/anatomy & histology/*surgery
    Swine
    
  • Roider, J. and Brinkmann, R. and Birngruber, R.: Selective retinal pigment epithelium laser treatment - Theoretical and clinical aspects. in Lasers in Ophthalmology - Basic, Diagnostic and Surgical Aspects, pp. 119-129, Kugler Publications, The Hague, The Netherlands, 2003
    BibTeX
    @inbook{Roider,
       author = {Roider, J. and Brinkmann, R. and Birngruber, R.},
       title = {Selective retinal pigment epithelium laser treatment -   Theoretical and clinical aspects},
       booktitle = {Lasers in Ophthalmology - Basic, Diagnostic and Surgical Aspects},
       editor = {Fankhauser, F. and Kwasniewska, S.},
       publisher = {Kugler Publications, The Hague, The Netherlands},
       pages = {119-129},
       year = {2003}
    }
    
  • Diddens, H. and Fischer, F. and Pottier, R. H.: In-vivo investigations on dye-enhanced photothermal tumor therapy with a naphthalocyanine derivative. Oftalmologia, no. 56, pp. 59-61, 2003
    BibTeX
    @article{Diddens,
       author = {Diddens, H. and Fischer, F. and Pottier, R. H.},
       title = {In-vivo investigations on dye-enhanced photothermal tumor therapy with a naphthalocyanine derivative},
       journal = {Oftalmologia},
       volume = {56},
       number = {1},
       pages = {59-61},
       note = {Diddens, Heyke
    Fischer, Frank
    Pottier, Roy H
    Romania
    Bucharest, Romania : 1990
    Oftalmologia. 2003;56(1):59-61.},
       abstract = {Chromophore-enhanced photothermal therapy involves the application of an exogenous chromophore in combination with irradiation, using an appropriate wavelength, exposure duration and sufficient irradiances. The chromophore palladium(II) octabutoxynaphthalocyanine (PdNc(OBu)8) accumulates at satisfactory concentrations and with good selectivity between both tumor and muscle and tumor and skin in tumor-bearing mice. In an attempt to thermally damage tumor tissue with concurrent sparing of adjacent normal tissue, the potential of PdNc(OBu)8 for photothermal therapy was investigated. Using a Balb/c mouse model with subcutaneously implanted EMT6 adenocarcinoma, 90-100 hours after intraperitoneal application of PdNc(OBu)8, the tumor and surrounding tissue were irradiated with a 826nm continuous-wave diode laser. The thermal effects on tumor and normal tissue were evaluated histologically. Our results indicate that after PdNc(OBu)8 administration and tumor irradiation using 5W/cm-for 100 seconds, pronounced selective heating of the tumor was achieved in mice, while in control animals merely an unspecific and marginal overall increase in temperature over the entire irradiation area was observed. Histological evaluation of treated areas indicated that the PdNc(OBu)8-targeted tumor tissue showed severe thermal damage while peripheral tissue like skin and muscle remained largely unaffected. This study shows the potential of creating localized thermal effects by using PdNc(OBu)8 and continuous-wave light for chromophore-enhanced photothermal therapy.},
       keywords = {Adenocarcinoma/*therapy
    Animals
    Disease Models, Animal
    Female
    Hindlimb
    Lasers
    Mice
    Mice, Inbred BALB C
    Organometallic Compounds/*therapeutic use
    Photochemotherapy/*methods
    Soft Tissue Neoplasms/*therapy
    Treatment Outcome},
       year = {2003}
    }
    
  • Framme, C. and Alt, C. and Schuele, G. and Brinkmann, R. and Birngruber, R. and Lin, C.: Threshold determinations for selective RPE laser treatment with a laser scanner in rabbits with different scan times in the microsecond regime. Investigative Ophthalmology & Visual Science, no. 44, pp. U663-U663, 2003
    BibTeX Link
    @article{Framme2003,
       author = {Framme, C. and Alt, C. and Schuele, G. and Brinkmann, R. and Birngruber, R. and Lin, C.},
       title = {Threshold determinations for selective RPE laser treatment with a laser scanner in rabbits with different scan times in the microsecond regime},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {44},
       pages = {U663-U663},
       note = {Suppl. 2
    709CK
    4865
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000184607002325},
       year = {2003},
       type = {Journal Article}
    }
    
  • Brinkmann, R. and Koop, N. and Ozdemir, M. and Alt, C. and Schule, G. and Lin, C. P. and Birngruber, R.: Targeting of the retinal pigment epithelium (RPE) by means of a rapidly scanned continuous wave (CW) laser beam. Lasers Surg Med, no. 32, pp. 252-64,
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R. and Koop, N. and Ozdemir, M. and Alt, C. and Schule, G. and Lin, C. P. and Birngruber, R.},
       title = {Targeting of the retinal pigment epithelium (RPE) by means of a rapidly scanned continuous wave (CW) laser beam},
       journal = {Lasers Surg Med},
       volume = {32},
       number = {4},
       pages = {252-64},
       note = {0196-8092 (Print)
    In Vitro
    
  • Teschner, S. and Noack, J. and Birngruber, R. and Schmidt-Erfurth, U.: Characterization of leakage activity in exudative chorioretinal disease with three-dimensional confocal angiography. Ophthalmology, no. 110, pp. 687-697, 2003
    BibTeX Link Link
    @article{Teschner2003,
       author = {Teschner, S. and Noack, J. and Birngruber, R. and Schmidt-Erfurth, U.},
       title = {Characterization of leakage activity in exudative chorioretinal disease with three-dimensional confocal angiography},
       journal = {Ophthalmology},
       volume = {110},
       number = {4},
       pages = {687-697},
       note = {673EE
    Times Cited:8
    Cited References Count:30},
       abstract = {Purpose: A novel angiographic technique providing topographic imaging of chorioretinal fluorescence is applied to the characterization of leakage dynamics in exudative chorioretinopathy. The three-dimensional imaging is evaluated with respect to results with conventional two-dimensional fluorescence angiography.
    Design: Prospective observational case series.
    Participants: Thirty eyes of 30 patients with different exudative maculopathies (pigment epithelium detachment, branch retinal vein occlusion, central serous chorioretinopathy, each n = 10) and 11 eyes of 10 patients with clinically normal appearance.
    Methods: Depth-resolved fluorescence angiography using a confocal scanning laser system was performed after complete ophthalmologic examination. The axial distribution of fluorescein and indocyanine green fluorescence at each x/y position within a tomographic scan of 32 images was analyzed. The chorioretinal fluorescence topography was reconstructed by localizing a defined threshold value of fluorescence and displayed as topographic relief. Qualitative description and quantitative measurements of exudation or structural alterations were performed topographically and conventionally.
    Main Outcome Measures. Qualitative and quantitative analysis of structural or exudative changes in time course in topographic illustration compared with conventional angiography.
    Results: Clinically physiologic eyes were presented topographically as a smooth concave surface of fluorescence with defined illustration of retinal vascular structures and the optic disc. Retinal vascular pathologic conditions induce a precisely demarcated pattern of intraretinal edema with a characteristic temporal evolution. In central serous retinopathy the underlying pathologic condition was identified as a perfusion defect, which was subsequently filled with a peak of exudation with differences in the time of maximum in fluorescein/indocyanine green angiography. Pigment epithelium detachment appeared as a high and well defined elevation, with the origin of exudation within the base of the detachment. Differences in the time of maximum prominence were found in indocyanine green angiography within the pigment epithelium detachment group.
    Conclusions: Confocal topographic angiography allows for the first time precise three-dimensional functional imaging of fundus alterations caused by leakage or barrier dysfunction. Compared with conventional angiography, depth-resolved angiographic imaging is less impaired by masking phenomena or low fluorescence intensity, which improves the diagnostic yield of angiography. The characterization and quantification of leakage activity is a promising tool in the assessment of exudative maculopathy. (C) 2003 by the American Academy of Ophthalmology.},
       keywords = {indocyanine green angiography
    scanning laser ophthalmoscope
    central serous chorioretinopathy
    choroidal neovascularization
    macular degeneration
    topographic angiography
    retinal thickness
    fluorescein
    videoangiography
    tomography},
       ISSN = {0161-6420},
       DOI = {Doi 10.1016/S6420(02)01972-3},
       url = {<Go to ISI>://WOS:000182566600026},
       year = {2003},
       type = {Journal Article}
    }
    
  • Vogel, Alfred and Venugopalan, Vasan: Kinetics of phase transitions in pulsed IR laser ablation of biological tissues. no. 4961, pp. 66-74, SPIE,
    BibTeX
    @inproceedings{Vogel,
       author = {Vogel, Alfred and Venugopalan, Vasan},
       title = {Kinetics of phase transitions in pulsed IR laser ablation of biological tissues},
       editor = {Steven, L. Jacques and Donald, D. Duncan and Sean, J. Kirkpatrick and Andres, Kriete},
       publisher = {SPIE},
       volume = {4961},
       pages = {66-74},
    }
    
  • Apitz, Ingo and Vogel, Alfred: Material ejection in Q-switched Er:YAG laser ablation of water, liver, and skin. no. 4961, pp. 48-59, SPIE,
    BibTeX
    @inproceedings{Apitz,
       author = {Apitz, Ingo and Vogel, Alfred},
       title = {Material ejection in Q-switched Er:YAG laser ablation of water, liver, and skin},
       editor = {Steven, L. Jacques and Donald, D. Duncan and Sean, J. Kirkpatrick and Andres, Kriete},
       publisher = {SPIE},
       volume = {4961},
       pages = {48-59},
    }
    
  • Huber, R and Moser, J.E. and Grutzet, M and Wachtveitl, J: Beyond vibrationally mediated electron transfer: coherent phenomena in a sub-10-femtosecond reaction regime. 2003 European Quantum Electronics Conference. EQEC 2003 (IEEE Cat No.03TH8665), pp. 158, IEEE, 2003
    BibTeX Link
    @Article{HU_2003_Huber_a,
      Title                    = {{Beyond vibrationally mediated electron transfer: coherent phenomena in a sub-10-femtosecond reaction regime}},
      Author                   = {Huber, R and Moser, J.E. and Grutzet, M and Wachtveitl, J},
      Journal                  = {2003 European Quantum Electronics Conference. EQEC 2003 (IEEE Cat No.03TH8665)},
      Year                     = {2003},
      Pages                    = {158},
    
      Doi                      = {10.1109/EQEC.2003.1314015},
      ISBN                     = {0-7803-7733-8},
      Publisher                = {IEEE},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=1314015}
    }
    
  • Huber, Robert and Moser, Jacques E and Gratzel, Michael and Wachtveitl, Josef L: Beyond vibrationally mediated electron transfer: interfacial charge injection on a sub-10-fs time scale. in Optical Science and Technology, SPIE`s 48th Annual Meeting, pp. 121-131, International Society for Optics and Photonics, dec,, 2003
    BibTeX Link
    @InProceedings{HU_2003_Huber_b,
      Title                    = {{Beyond vibrationally mediated electron transfer: interfacial charge injection on a sub-10-fs time scale}},
      Author                   = {Huber, Robert and Moser, Jacques E and Gratzel, Michael and Wachtveitl, Josef L},
      Booktitle                = {Optical Science and Technology, SPIE's 48th Annual Meeting},
      Year                     = {2003},
      Editor                   = {Lian, Tianquan and Dai, Hai-Lung},
      Month                    = dec,
      Pages                    = {121--131},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.506722},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=828500}
    }

2002

  • Muller-Velten, R. and Hillmann, K. and Birngruber, R. and Noack, J. and Schmidt-Erfurth, U.: Topographic imaging and quantification of retinal vascular leakage in venous branch occlusion. Investigative Ophthalmology & Visual Science, no. 43, pp. U805-U805, 2002
    BibTeX Link
    @article{Müller-Velten2002,
       author = {Muller-Velten, R. and Hillmann, K. and Birngruber, R. and Noack, J. and Schmidt-Erfurth, U.},
       title = {Topographic imaging and quantification of retinal vascular leakage in venous branch occlusion},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {43},
       pages = {U805-U805},
       note = {Suppl. 2
    709CG
    2859
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000184606700021},
       year = {2002},
       type = {Journal Article}
    }
    
  • Brinkmann, R and Koop, N and Özdemir, M and Alt, C and Schüle, G and Lin, C P and Birngruber, R: Selective damage of pigmented cells by means of a rapidly scanned cw laser beam. Proc SPIE, no. 4617, pp. 134-140, 2002
    BibTeX
    @article{Brinkmann2002,
       author = {Brinkmann, R  and Koop, N and Özdemir, M  and Alt, C and Schüle, G and Lin, C P and Birngruber, R},
       title = {Selective damage of pigmented cells by means of a rapidly scanned cw laser beam},
       journal = {Proc SPIE},
       volume = {4617},
       pages = {134-140},
       year = {2002},
       type = {Journal Article}
    }
    
  • Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.: High Precision Cell Surgery with Nanoparticles?. Med Laser Appl, no. 17, pp. 9-14, 2002
    BibTeX
    @article{Hüttmann2002,
       author = {Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.},
       title = {High Precision Cell Surgery with Nanoparticles?},
       journal = {Med Laser Appl},
       volume = {17},
       pages = {9-14},
       year = {2002},
       type = {Journal Article}
    }
    
  • Alt, C and Framme, C and Schnell, S and Schuele, G and Brinkmann, R and Lin, C P: In vivo and in vitro Selective Targeting of the Retinal Pigment Epithelium (RPE) Using a Laser-Scanning Device. in Proc SPIE, no. 4611, pp. 59-63,
    BibTeX
    @inproceedings{Alt2002,
       author = {Alt, C and Framme, C and Schnell, S and Schuele, G and Brinkmann, R and Lin, C P},
       title = {In vivo and in vitro Selective Targeting of the Retinal Pigment Epithelium (RPE) Using a Laser-Scanning Device},
       booktitle = {Proc SPIE},
       volume = {4611},
       pages = {59-63},
       type = {Conference Proceedings}
    }
    
  • Framme, C and Brinkmann, R and Birngruber, R and Roider, J: Autofluorescence imaging after selective RPE treatment in macular diseases and clinical outcome: a pilot study. Br J Ophthalmol, no. 86, pp. 1099-1106, 2002
    BibTeX
    @article{Framme2002,
       author = {Framme, C and Brinkmann, R and Birngruber, R and Roider, J},
       title = {Autofluorescence imaging after selective RPE treatment in macular diseases and clinical outcome: a pilot study},
       journal = {Br J Ophthalmol},
       volume = {86},
       pages = {1099-1106},
       year = {2002},
       type = {Journal Article}
    }
    
  • Y, Miura and T, Yokoyma and H, Tabuchi and M, Yamaguchi and N, Hosoda and Y, Miyata: Long-term outcome of juvenile-onset myasthenia ocularis treated by systemic corticosteroid. Japanese review of clinical ophthalmology.. Japanese Review of Clinical Ophthalmology, no. 96, pp. 1133-1135, 2002
    BibTeX
    @article{Miura2002,
       author = {Y, Miura and T, Yokoyma and H, Tabuchi and M, Yamaguchi and N, Hosoda and Y, Miyata},
       title = {Long-term outcome of juvenile-onset myasthenia ocularis treated by systemic corticosteroid. Japanese review of clinical ophthalmology. },
       journal = {Japanese Review of Clinical Ophthalmology},
       volume = {96},
       number = {11},
       pages = {1133-1135},
       year = {2002}
    }
    
  • Vogel, A. and Schmidt, P. and Flucke, B.: Minimization of thermomechanical side effects and increase of ablation efficiency in IR ablation by use of multiply Q-switched laser pulses. SPIE Proc. Laser Tissue Interaction XIII, no. 4617A, 2002
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Schmidt, P. and Flucke, B.},
       title = {Minimization of thermomechanical side effects and increase of ablation efficiency in IR ablation by use of multiply Q-switched laser pulses},
       journal = {SPIE Proc. Laser Tissue Interaction XIII},
       volume = {4617A},
       year = {2002}
    }
    
  • Hoerauf, H. and Scholz, C. and Koch, P. and Engelhardt, R. and Laqua, H. and Birngruber, R.: Transscleral optical coherence tomography: a new imaging method for the anterior segment of the eye. Arch Ophthalmol, no. 120, pp. 816-9, 2002
    BibTeX
    @article{Hoerauf2002,
       author = {Hoerauf, H. and Scholz, C. and Koch, P. and Engelhardt, R. and Laqua, H. and Birngruber, R.},
       title = {Transscleral optical coherence tomography: a new imaging method for the anterior segment of the eye},
       journal = {Arch Ophthalmol},
       volume = {120},
       number = {6},
       pages = {816-9},
       note = {0003-9950 (Print)
    Journal Article},
       abstract = {OBJECTIVE: To present a new imaging method for the anterior segment of the eye. METHODS: Transscleral optical coherence tomographic images were generated in healthy volunteers using a slitlamp-adapted prototype equipped with a superluminescence diode with an infrared wavelength of 1310 nm. RESULTS: The optical coherence tomographic system used allowed penetration of human sclera in vivo and high-resolution, cross-sectional imaging of the anterior chamber angle and the ciliary body. CONCLUSION: The 1310-nm optical coherence tomographic image shows a significant potential as a noninvasive diagnostic tool for the anterior segment of the eye.},
       keywords = {Anatomy, Cross-Sectional/*methods
    Anterior Eye Segment/*anatomy & histology
    *Diagnostic Techniques, Ophthalmological
    Humans
    Interferometry
    Light
    Sclera
    Tomography/methods},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12049589},
       year = {2002},
       type = {Journal Article}
    }
    
  • Venugopalan, V. and Guerra, A. and Nahen, K. and Vogel, A.: Role of Laser-Induced Plasma Formation in Pulsed Cellular Microsurgery and Micromanipulation. Phys Rev Let, no. 88, pp. 078103 (1-4), 2002
    BibTeX
    @article{Venogupalan,
       author = {Venugopalan, V. and Guerra, A. and Nahen, K. and Vogel, A.},
       title = {Role of Laser-Induced Plasma Formation in Pulsed Cellular Microsurgery and Micromanipulation},
       journal = {Phys Rev Let},
       volume = {88},
       number = {7},
       pages = {078103 (1-4)},
       year = {2002}
    }
    
  • Framme, C. and Schuele, G. and Roider, J. and Kracht, D. and Birngruber, R. and Brinkmann, R.: Threshold determinations for selective retinal pigment epithelium damage with repetitive pulsed microsecond laser systems in rabbits. Ophthalmic Surgery and Lasers, no. 33, pp. 400-409, 2002
    BibTeX
    @article{Framme2002,
       author = {Framme, C. and Schuele, G. and Roider, J. and Kracht, D. and Birngruber, R. and Brinkmann, R.},
       title = {Threshold determinations for selective retinal pigment epithelium damage with repetitive pulsed microsecond laser systems in rabbits},
       journal = {Ophthalmic Surgery and Lasers},
       volume = {33},
       number = {5},
       pages = {400-409},
       note = {596HY
    Times Cited:18
    Cited References Count:28},
       abstract = {BACKGROUND AND OBJECTIVE: In both clinical and animal studies, it has been shown that repetitive short laser pulses can cause selective retinal pigment epithelium damage (RPE) with sparing of photoreceptors. Our purpose was to determine the ophthalmoscopic and angiographic damage thresholds as a function of pulse durations by using different pulsed laser systems to optimize treatment modalities.
    MATERIALS AND METHODS: Chinchilla-breed rabbits were narcotized and placed in a special holding system. Laser lesions were applied using a commercial laser slit lamp, contact lens, and irradiation with a frequency-doubled Nd:YLF laser (wavelength: 527 nm; repetition rate: 500 Hz; number of pulses: 100; pulse duration: 5 mus, 1.7 mus, 200 ns) and an argon-ion laser (514 nm, 500 Hz, 100 pulses, 5 mus and 200 ins). In all eyes, spots with different energies were placed into the regio macularis with a diameter of 102 mum (tophat profile). After treatment, fundus photography and fluorescein angiography were performed and radiant exposure for ED50 damage determined. Speckle measurements at the fiber tips were performed to determine intensity peaks in the beam profile.
    RESULTS: Using the Nd:YLF laser system, the ophthalmoscopic ED50 threshold energies were 25.4 lJ (5 mus), 32 muJ (1.7 mus), and 30 muJ (200 ns). The angiographic ED50 thresholds were 13.4 muJ (5 mus), 9.2 muJ (1.7 mus), and 6.7 muJ (200 ns). With the argon laser, the angiographic threshold for 5 mus pulses was 5.5 muJ. The ophthalmoscopic threshold could not be determined because of a lack of power; however, it was > 12 muJ. For 200 ms, the ED50 radiant exposures were 20.4 mW ophthalmoscopically and 19.2 mW angiographically. Speckle factors were found to be 1.225 for the Nd:YLF and 3.180 for the argon laser. Thus, the maximal ED50-threshold radiant exposures for the Nd:YLF were calculated to be 362 mJ/cm(2) (5 mus), 478 mJ/cm(2) (1.7 mus), and 438 mJ/cm(2) (200 ns) ophthalmoscopically. Angiographically, the thresholds were 189 mJ/cm(2) (5 mus), 143 mJ/cm(2) (1.7 mus), and 97 mJ/cm(2) (200 ns). For the argon laser, the maximal ED50 radiant exposure threshold was 170 mJ/cm(2) angiographically.
    CONCLUSION: The gap between the angiographic and the ophthalmoscopic thresholds for the 200 ns regime (4.5 times above angiographic ED50) was wider than for the 1.7 mus regime (3.3 times above the angiographic ED50). This would suggest the appropriate treatment would be 200 ns pulses. However, histologies have yet to prove that nonvisible mechanical effects increase with shorter pulse durations and could reduce the "therapeutic window." When comparing the thresholds with 5 mus pulses from the argon and Nd:YLF laser, it demonstrates that intensity modulations in the beam profile must be considered.},
       keywords = {primate eye
    photocoagulation
    neovascularization
    nanosecond
    lesions
    model},
       ISSN = {0022-023X},
       url = {<Go to ISI>://WOS:000178160100008},
       year = {2002},
       type = {Journal Article}
    }
    
  • Brinkmann, R. and Koop, N. and Oezdemir, M. and Alt, C. and Schuele, G. and Lin, C. P. and Birngruber, R.: Selective RPE damage by means of a rapidly scanned cw laser beam. Investigative Ophthalmology & Visual Science, no. 43, pp. U595-U595, 2002
    BibTeX Link
    @article{Brinkmann2002,
       author = {Brinkmann, R. and Koop, N. and Oezdemir, M. and Alt, C. and Schuele, G. and Lin, C. P. and Birngruber, R.},
       title = {Selective RPE damage by means of a rapidly scanned cw laser beam},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {43},
       pages = {U595-U595},
       note = {Suppl. 1
    709CF
    2535
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000184606602467},
       year = {2002},
       type = {Journal Article}
    }
    
  • Vogel, Alfred . and Schweiger, P. and Frieser, A. and Asiyo, M. and Birngruber, R.: Intraocular Nd: YAG Laser Surgery: Light-Tissue Interaction, Damage Range, and Reduction of Collateral Effects. IEEE J Quant Electr, no. 26, pp. 2241-2260, 2002
    BibTeX
    @article{Vogel2002,
       author = {Vogel, Alfred . and Schweiger, P. and Frieser, A. and Asiyo, M. and Birngruber, R.},
       title = {Intraocular Nd: YAG Laser Surgery: Light-Tissue Interaction, Damage Range, and Reduction of Collateral Effects},
       journal = {IEEE J Quant Electr},
       volume = {26},
       pages = {2241-2260},
       year = {2002},
       type = {Journal Article}
    }
    
  • Nahen, K. and Vogel, A.: Plume dynamics and shielding by the ablation plume during Er:YAG laser ablation. Journal of Biomedical Optics, no. 7, pp. 165-178, 2002
    BibTeX
    @article{Nahen,
       author = {Nahen, K. and Vogel, A.},
       title = {Plume dynamics and shielding by the ablation plume during Er:YAG laser ablation},
       journal = {Journal of Biomedical Optics},
       volume = {7},
       number = {2},
       pages = {165-178},
       year = {2002}
    }
    
  • Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.: High Precision Cell Surgery with Nanoparticles?. Med Laser Appl, no. 17, pp. 9-14, 2002
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.},
       title = {High Precision Cell Surgery with Nanoparticles?},
       journal = {Med Laser Appl},
       volume = {17},
       pages = {9-14},
       year = {2002}
    }
    
  • Hüttmann, G and Houserek, S. and Diddens, H. and Hendrich, C.: Photodynamische Therapie in der Orthopädie. pp. 344-352, 2002
    BibTeX
    @misc{Hüttmann,
       author = {Hüttmann, G and Houserek, S. and Diddens, H. and Hendrich, C.},
       title = {Photodynamische Therapie in der Orthopädie},
       pages = {344-352},
       year = {2002}
    }
    
  • Schmidt-Erfurth, U. M. and Ahlswede, W. and Michels, S. and Birngruber, R. and Laqua, H.: Three-dimensional analysis of photodynamic therapy (PDT)-induced vascular effects in choroidal neovascularization (CNV) and choroid: A two year follow-up. Investigative Ophthalmology & Visual Science, no. 43, pp. U1143-U1143, 2002
    BibTeX Link
    @article{Schmidt-Erfurth2002,
       author = {Schmidt-Erfurth, U. M. and Ahlswede, W. and Michels, S. and Birngruber, R. and Laqua, H.},
       title = {Three-dimensional analysis of photodynamic therapy (PDT)-induced vascular effects in choroidal neovascularization (CNV) and choroid: A two year follow-up},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {43},
       pages = {U1143-U1143},
       note = {Suppl. 2
    709CG
    3978
    Times Cited:0
    Cited References Count:0},
       ISSN = {0146-0404},
       url = {<Go to ISI>://WOS:000184606701132},
       year = {2002},
       type = {Journal Article}
    }
    
  • Framme, C. and Schuele, G. and Roider, J. and Kracht, D. and Birngruber, R. and Brinkmann, R.: Threshold determinations for selective retinal pigment epithelium damage with repetitive pulsed microsecond laser systems in rabbits. no. 33, pp. 400-9, Sep-Oct, 2002
    BibTeX
    @misc{Framme,
       author = {Framme, C. and Schuele, G. and Roider, J. and Kracht, D. and Birngruber, R. and Brinkmann, R.},
       title = {Threshold determinations for selective retinal pigment epithelium damage with repetitive pulsed microsecond laser systems in rabbits},
       volume = {33},
       number = {5},
       pages = {400-9},
       month = {Sep-Oct},
       note = {1082-3069 (Print)
    Comparative Study
    Journal Article},
       abstract = {BACKGROUND AND OBJECTIVE: In both clinical and animal studies, it has been shown that repetitive short laser pulses can cause selective retinal pigment epithelium damage (RPE) with sparing of photoreceptors. Our purpose was to determine the ophthalmoscopic and angiographic damage thresholds as a function of pulse durations by using different pulsed laser systems to optimize treatment modalities. MATERIALS AND METHODS: Chinchilla-breed rabbits were narcotized and placed in a special holding system. Laser lesions were applied using a commercial laser slit lamp, contact lens, and irradiation with a frequency-doubled Nd:YLF laser (wave-length: 527 nm; repetition rate: 500 Hz; number of pulses: 100; pulse duration: 5 micros, 1.7 micros, 200 ns) and an argon-ion laser (514 nm, 500 Hz, 100 pulses, 5 micros and 200 ms). In all eyes, spots with different energies were placed into the regio macularis with a diameter of 102 microm (tophat profile). After treatment, fundus photography and fluorescein angiography were performed and radiant exposure for ED50 damage determined. Speckle measurements at the fiber tips were performed to determine intensity peaks in the beam profile. RESULTS: Using the Nd:YLF laser system, the ophthalmoscopic ED50 threshold energies were 25.4 microJ (5 micros), 32 microJ (1.7 micros), and 30 microJ (200 ns). The angiographic ED50 thresholds were 13.4 microJ (5 micros), 9.2 microJ (1.7 micros), and 6.7 microJ (200 ns). With the argon laser, the angiographic threshold for 5 micros pulses was 5.5 microJ. The ophthalmoscopic threshold could not be determined because of a lack of power; however, it was > 12 microJ. For 200 ms, the ED50 radiant exposures were 20.4 mW ophthalmoscopically and 19.2 mW angiographically. Speckle factors were found to be 1.225 for the Nd:YLF and 3.180 for the argon laser. Thus, the maximal ED50 -threshold radiant exposures for the Nd:YLF were calculated to be 362 mJ/cM2 (5 micros), 478 mJ/cm2 (1.7 micros), and 438 mJ/cm2 (200 ns) ophthalmoscopically. Angiographically, the thresholds were 189 mJ/cm2 (5 micros), 143 mJ/cm2 (1.7 micros), and 97 mJ/cm2 (200 ns). For the argon laser, the maximal ED50 radiant exposure threshold was 170 mJ/cm2 angiographically. CONCLUSION: The gap between the angiographic and the ophthalmoscopic thresholds for the 200 ns regime (4.5 times above angiographic ED50) was wider than for the 1.7 micros regime (3.3 times above the angiographic ED50). This would suggest the appropriate treatment would be 200 ns pulses. However, histologies have yet to prove that nonvisible mechanical effects increase with shorter pulse durations and could reduce the "therapeutic window." When comparing the thresholds with 5 micros pulses from the argon and Nd:YLF laser, it demonstrates that intensity modulations in the beam profile must be considered.},
       keywords = {Animals
    Eye Injuries/diagnosis/*etiology
    Fluorescein Angiography
    Laser Coagulation/*adverse effects
    Ophthalmoscopy
    Photography
    Pigment Epithelium of Eye/*injuries
    Rabbits
    Retina/*surgery
    Threshold Limit Values
    Time Factors},
       year = {2002}
    }
    
  • Hoerauf, H. and Winkler, J. and Scholz, C. and Wirbelauer, C. and Gordes, R. S. and Koch, P. and Engelhardt, R. and Laqua, H. and Birngruber, R.: Transscleral optical coherence tomography-an experimental study in ex-vivo human eyes. Lasers Surg Med, no. 30, pp. 209-15, 2002
    BibTeX
    @article{Hoerauf2002,
       author = {Hoerauf, H. and Winkler, J. and Scholz, C. and Wirbelauer, C. and Gordes, R. S. and Koch, P. and Engelhardt, R. and Laqua, H. and Birngruber, R.},
       title = {Transscleral optical coherence tomography--an experimental study in ex-vivo human eyes},
       journal = {Lasers Surg Med},
       volume = {30},
       number = {3},
       pages = {209-15},
       note = {0196-8092 (Print)
    Journal Article},
       abstract = {BACKGROUND AND OBJECTIVE: To evaluate the potentials of a 1310-nm optical coherence tomography (OCT) system to penetrate the highly backscattering sclera in enucleated human eyes and provide visualization of intraocular structures by transscleral imaging. STUDY DESIGN/MATERIALS AND METHODS: OCT-images were generated by an experimental prototype (Medical Laser Center, Lubeck, Germany) using a superluminescence diode with a wavelength of 1310 nm. OCT-images were taken from two enucleated human eyes using 100-200 axial scans with 60 Hz line scan frequency and compared to subsequent histologic sections. RESULTS: Transscleral OCT allowed penetration of the sclera and the anterior chamber angle could be completely identified. Some change within the anterior eye segment could be demonstrated with high accuracy. Additionally, limited demonstration of the ciliary body region was achieved. Due to limited signal intensity no detailed imaging of the pars plana and pars plicata region was possible. However, more posterior measurements allowed transscleral visualization of a retinal detachment. CONCLUSIONS: OCT using lightsources with a wavelength longer than that used in conventional OCT provides a promising imaging technique at high resolution allowing transscleral imaging of the anterior eye segment.},
       keywords = {Anterior Chamber/cytology
    Anterior Eye Segment/cytology
    Ciliary Body/cytology
    Equipment Design
    Eye/*cytology
    Humans
    Tomography/instrumentation/*methods},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11891740},
       year = {2002},
       type = {Journal Article}
    }
    
  • Framme, C. and Brinkmann, R. and Birngruber, R. and Roider, J.: Autofluorescence imaging after selective RPE laser treatment in macular diseases and clinical outcome: a pilot study. British Journal of Ophthalmology, no. 86, pp. 1099-1106, 2002
    BibTeX Link
    @article{Framme2002,
       author = {Framme, C. and Brinkmann, R. and Birngruber, R. and Roider, J.},
       title = {Autofluorescence imaging after selective RPE laser treatment in macular diseases and clinical outcome: a pilot study},
       journal = {British Journal of Ophthalmology},
       volume = {86},
       number = {10},
       pages = {1099-1106},
       note = {595YE
    Times Cited:47
    Cited References Count:35},
       abstract = {Aim: Selective retinal pigment epithelium (RPE) laser treatment is a new technique which selectively damages the RPE while sparing the neural retina. One difficulty is the inability to visualise the laser lesions, The aim of the study was to investigate whether fundus autofluorescence (AF) is changed because of the RPE damage, and thus might be used for treatment control. Additionally, the clinical course of patients with various macular diseases was evaluated.
    Methods: 26 patients with macular diseases (diabetic maculopathy (DMP), soft drusen maculopathy (AMD), and central serous retinopathy (CSR)) were treated and followed up for at least 6 months. Treatment was performed with a train of repetitive short laser pulses (800 ns) of a frequency doubled Nd:YAG laser (parameters: 532 nm, 50 and 500 pulses at 100 and 500 Hz, retinal spot diameter 200 pm, pulse energies 75-175 muJ). AF was excited by 488 nm and detected by a barrier filter at 500 nm (HRA, Heidelberg Engineering, Germany). Patients were examined by ophthalmoscopy, fluorescein angiography, and autofluorescence measurements at various times after treatment (10 minutes, 1 hour, 1 and 6 weeks, 3, 6, and 12 months).
    Results: Fluorescein angiography showed leakage from the irradiated areas for about I week after treatment. None of the laser lesions was ophthalmoscopically visible during treatment. Identification of the lesions was possible by AF imaging showing an intensity decay in the irradiated area in 22 out of 26 patients, predominantly in patients with CSR and AMD. Lesions could be identified 10 minutes after treatment as hypoautofluorescent spots, which were more pronounced I hour later. During follow up the laser spots became hyperautofluorescent. In patients with DMP some AF images were less helpful because of diffuse oedema and larger retinal thickness. In these cases ICG angiography was able to confirm therapeutic success very well. Most of the patients have had benefit from the treatment, with best results obtained for CSR patients.
    Conclusion: Imaging of non-visible selective RPE laser effects can be achieved by AF measurements predominantly in patients without retinal oedema. Therefore, AF may replace invasive fluorescein angiography in many cases to verify therapeutic laser success, Selective laser treatment has the potential to improve the prognosis of macular diseases without the risk of laser scotomas.},
       keywords = {retinal-pigment epithelium
    primate retina
    fundus autofluorescence
    scanning ophthalmoscope
    photocoagulation
    lipofuscin
    krypton
    fluorescence
    melanin
    lesions},
       ISSN = {0007-1161},
       DOI = {DOI 10.1136/bjo.86.10.1099},
       url = {<Go to ISI>://WOS:000178135200010},
       year = {2002},
       type = {Journal Article}
    }
    
  • Framme, C and Brinkmann, R and Birngruber, R and Roider, J: Autofluorescence imaging after selective RPE treatment in macular diseases and clinical outcome: a pilot study. Br J Ophthalmol, no. 86, pp. 1099-1106, 2002
    BibTeX
    @article{Framme,
       author = {Framme, C and Brinkmann, R and Birngruber, R and Roider, J},
       title = {Autofluorescence imaging after selective RPE treatment in macular diseases and clinical outcome: a pilot study},
       journal = {Br J Ophthalmol},
       volume = {86},
       pages = {1099-1106},
       year = {2002}
    }
    
    
  • Wirbelauer, C. and Scholz, C. and Hoerauf, H. and Pham, D. T. and Laqua, H. and Birngruber, R.: Noncontact corneal pachymetry with slit lamp-adapted optical coherence tomography. American Journal of Ophthalmology, no. 133, pp. 444-450, 2002
    BibTeX Link
    @article{Wirbelauer2002,
       author = {Wirbelauer, C. and Scholz, C. and Hoerauf, H. and Pham, D. T. and Laqua, H. and Birngruber, R.},
       title = {Noncontact corneal pachymetry with slit lamp-adapted optical coherence tomography},
       journal = {American Journal of Ophthalmology},
       volume = {133},
       number = {4},
       pages = {444-450},
       note = {538DE
    Times Cited:73
    Cited References Count:35},
       abstract = {PURPOSE: The purpose of this study was to determine the accuracy, the reproducibility, and the limits of agreement of noncontact central corneal thickness measurement with slit lamp adapted optical coherence tomography (OCT). DESIGN: Nonrandomized comparative clinical trial.
    METHODS: In a prospective comparative observational study, a total of 108 consecutive patients ( 108 eyes) with normal corneas (92 eyes) and different corneal alters ations (16 eyes) participated. Six sequential measure. ments of the central corneal thickness with slit lamp-adapted OCT and with ultrasound (US) pachymetry at 1640 ms(-1) were performed. The main outcome measures were accuracy, reproducibility assessed with precision and coefficient of variation (CV), and limits of agreement of central corneal thickness measurement.
    RESULTS: The mean central corneal thickness values were 541 +/- 43 mum (OCT) and 549 +/- 44 mum (US) with a mean precision of +/- 5.8 mum (CV 1.08%) and of +/- 4.0 mum (CV 0.73%), respectively. The method comparison revealed equivalence (+/- 2SD) in the 5% range with a mean difference between both methods of 7.9 mum (1-45%). The relative error was 8.7 mum (1.6%), which corresponded to limits of agreement (+/- 2SD) ranging from -9.5 mum to 25.3 mum.
    CONCLUSIONS: Central corneal pachymetry with slit lamp,adapted OCT revealed, for clinical purposes, an excellent accuracy and reproducibility with a high degree of agreement compared with US pachymetry. Thus, the presented OCT system seems to be a promising diagnostic modality to objectively measure corneal thickness in a convenient noncontact mode. (C) 2002 by Elsevier Science Inc. All rights reserved.},
       keywords = {in-situ keratomileusis
    anterior segment
    human-eye
    thickness
    interferometry
    regression
    precision},
       ISSN = {0002-9394},
       DOI = {Pii S0002-9394(01)01425-8
    Doi 10.1016/S0002-9394(01)01425-8},
       url = {<Go to ISI>://WOS:000174798200002},
       year = {2002},
       type = {Journal Article}
    }
    
  • Brujan, EA. and Keen, G.S. and Vogel, A. and Blake, J.R.: The final stage of the collapse of a cavitation bubble close to a rigid boundary. Phys Fluids, no. 14, pp. 85-92, 2002
    BibTeX
    @article{Brujan2002,
       author = {Brujan, EA. and Keen, G.S. and Vogel, A. and Blake, J.R.},
       title = {The final stage of the collapse of a cavitation bubble close to a rigid boundary},
       journal = {Phys Fluids},
       volume = {14},
       pages = {85-92},
       year = {2002}
    }
    
    
  • Vogel, A. and Schmidt, P. and Flucke, B.: Minimization of thermomechanical side effects and increase of ablation efficiency in IR ablation by use of multiply Q-switched laser pulses. SPIE Proc. Laser Tissue Interaction XIII, no. 4617A, 2002
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Schmidt, P. and Flucke, B.},
       title = {Minimization of thermomechanical side effects and increase of ablation efficiency in IR ablation by use of multiply Q-switched laser pulses},
       journal = {SPIE Proc. Laser Tissue Interaction XIII},
       volume = {4617A},
       year = {2002}
    }
    
  • Vogel, A. and Hüttmann, G. and Paltauf, G.: Low-density plasmas below the optical breakdown threshold: potential hazard for multiphoton microscopy, and a tool for the manipulation of intracellular events. Proc SPIE Multiphoton Microscopy in the Biomedical Sciences II, no. 4620, pp. 202-216, 2002
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Hüttmann, G. and Paltauf, G.},
       title = {Low-density pl