The research group led by Robert Huber conducts research in the fields of optical coherence tomography (OCT), non-linear imaging and laser physics. Here, work is mainly done on novel pico second lasers and on Fourier domain mode-locked (FDML) lasers. This laser concept was developed by Robert Huber and enables the realization of particularly fast tunable laser light sources. The research focus here is on further technological development, understanding of the physical processes and also on the implementation of FDML lasers for OCT applications. Besides OCT, FDML lasers are also used for nonlinear imaging and spectroscopy.
Another focus is on optical coherence tomography with tunable light sources (swept source OCT, SS-OCT). Here, among other applications, the in-house developed FDML lasers are used for ultrafast imaging to generate cross-sectional images of biological tissues such as skin or eye. Due to the high tuning rate, applications like VR-OCT, which displays entire volumes in a virtual environment with real-time video repetition rates, are possible.
With non-linear optical imaging, the group is pursuing further imaging techniques. Research areas are in time-encoded (TICO) Raman spectroscopy and microscopy, two-photon fluorescence microscopy (TPEF), and two-photon single-pulse fluorescence lifetime imaging (SP-FLIM). These techniques use novel pico second lasers that are also being researched and developed in the group.
Our main research topics:
- Fourier Domain Mode Locked (FDML) lasers - laser physics, technology and application.
- Optical Coherence Tomography (OCT) - applications of MHz-OCT on skin and eye
- Non-linear microscopy and spectroscopy
- Pico second lasers
Publications
2023
Characterization of brain tumor tissue by time-resolved, phase-sensitive optical coherence elastography at 3.2 MHz line rate, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 123680F.
DOI: | 10.1117/12.2648301 |
Bibtex: | @inproceedings{10.1117/12.2648301, author = {Sazgar Burhan and Nicolas Detrez and Katharina Rewerts and Madita G{\"o}b and Christian Hagel and Matteo Mario Bonsanto and Dirk Theisen-Kunde and Robert Huber and Ralf Brinkmann}, title = {{Characterization of brain tumor tissue by time-resolved, phase-sensitive optical coherence elastography at 3.2 MHz line rate}}, volume = {12368}, booktitle = {Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI}, editor = {Caroline Boudoux and James W. Tunnell}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {123680F}, abstract = {Optical coherence elastography (OCE) offers the possibility of obtaining the mechanical behavior of a tissue. When also using a non-contact mechanical excitation, it mimics palpation without interobserver variability. One of the most frequently used techniques is phase-sensitive OCE. Depending on the system, depth-resolved changes in the sub-µm to nm range can be detected and visualized volumetrically. Such an approach is used in this work to investigate and detect transitions between healthy and tumorous brain tissue as well as inhomogeneities in the tumor itself to assist the operating surgeon during tumor resection in the future. We present time-resolved, phase-sensitive OCE measurements on various ex vivo brain tumor samples using an ultra-fast 3.2 MHz swept-source optical coherence tomography (SS-OCT) system with a frame rate of 2.45 kHz. 4 mm line scans are acquired which, in combination with the high imaging speed, allow monitoring and investigation of the sample's behavior in response to the mechanical load. Therefore, an air-jet system applies a 200 ms short air pulse to the sample, whose non-contact property facilitates the possibility for future in vivo measurements. Since we can temporally resolve the response of the sample over the entire acquisition time, the mechanical properties are evaluated at different time points with depth resolution. This is done by unwrapping the phase data and performing subsequent assessment. Systematic ex vivo brain tumor measurements were conducted and visualized as distribution maps. The study outcomes are supported by histological analyses and examined in detail.}, keywords = { Optical Coherence Tomography, Optical Coherence Elastography, Phase-sensitive OCT, Fourier Domain Mode Locking, Brain Tumor, Phase Unwrapping, Tissue Characterization, Biomechanics}, year = {2023}, doi = {10.1117/12.2648301}, URL = {https://doi.org/10.1117/12.2648301} } |
900 nm swept source FDML laser with kW peak power, in Fiber Lasers XX: Technology and Systems , V. R. Supradeepa, Eds. SPIE, 032023. pp. 124001I.
DOI: | 10.1117/12.2649663 |
Bibtex: | @inproceedings{10.1117/12.2649663, author = {Philipp Lamminger and Hubertus Hakert and Simon Lotz and Jan Philip Kolb and Tonio Kutscher and Sebastian Karpf and Robert Huber}, title = {{900 nm swept source FDML laser with kW peak power}}, volume = {12400}, booktitle = {Fiber Lasers XX: Technology and Systems}, editor = {V. R. Supradeepa}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {124001I}, abstract = {A wavelength agile 900 nm 2.5 kW peak power fiber laser is created by four-wave mixing (FWM) in a photonic crystal fiber (PCF), while amplifying a 1300 nm Fourier-domain mode-locked (FDML) laser. The FWM process is pumped by a home-built 1064 nm master oscillator power amplifier (MOPA) laser and seeded by a home-built 1300 nm FDML laser, generating high power pulses at wavelengths, where amplification by active fiber media is difficult. The 900 nm pulses have a spectral linewidth of 70 pm, are tunable over 54 nm and have electronic pulse-to-pulse tuning capability. These pulses can be used for nonlinear imaging like two-photon or coherent anti-Stokes Raman microscopy (CARS) microscopy including spectro-temporal laser imaging by diffracted excitation (SLIDE) and time-encoded (Tico) stimulated Raman microscopy.}, keywords = {Fourier domain mode locking, FDML, Raman, two photon microscopy, SLIDE, 900 nm, fiber laser, photonic crystal fiber, swept source}, year = {2023}, doi = {10.1117/12.2649663}, URL = {https://doi.org/10.1117/12.2649663} } |
850 nm FDML: performance and challenges, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 032023. pp. 1236705.
DOI: | 10.1117/12.2649646 |
Bibtex: | @inproceedings{10.1117/12.2649646, author = {M. Klufts and S. Lotz and M. A. Bashir and T. Pfeiffer and A. Mlynek and W. Wieser and A. Chamorovskiy and V. Shidlovski and R. Huber}, title = {{850 nm FDML: performance and challenges}}, volume = {12367}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1236705}, abstract = {We demonstrate a Fourier domain mode locked (FDML) laser centered around 850 nm with a sweeping range of 50 nm, a fundamental repetition rate of 2×416 kHz and an output power of 2 mW. A new cavity design using three chirped Fiber Bragg gratings is required to overcome sweeping limitations caused by high dispersion. Other solutions to address challenges such as high loss and high polarization mode dispersion will be discussed along with performance. A main application of this laser will be retinal imaging, but it might also be applicable for TiCo-Raman and SLIDE microscopy. }, keywords = {Swept source, FDML, Laser, Ophthalmic imaging, OCT, 800 nm, retinal imaging, light sources}, year = {2023}, doi = {10.1117/12.2649646}, URL = {https://doi.org/10.1117/12.2649646} } |
1190 nm Fourier domain mode locked (FDML) laser for optical coherence tomography (OCT), in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 032023. pp. 1236707.
DOI: | 10.1117/12.2652884 |
Bibtex: | @inproceedings{10.1117/12.2652884, author = {M. A. Bashir and S. Lotz and M. Klufts and I. Krestnikov and C. Jirauschek and R. Huber}, title = {{1190 nm Fourier domain mode locked (FDML) laser for optical coherence tomography (OCT)}}, volume = {12367}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1236707}, abstract = {We demonstrate a Fourier domain mode locked (FDML) laser centered at 1190 nm with 2×410 kHz sweep repetition rate, a sweeping range of 100 nm and 2.5 mW output power. The laser is based on a quantum dot-semiconductor optical amplifier with small linewidth enhancement factor. The laser could be used as a probe laser in stimulated Raman scattering microscopy and it may be attractive for optical coherence tomography due to low water absorption and the spectral signature of lipids around 1200nm. Moreover, it is ideal to close the gap between FDML lasers at 1064 nm and 1300 nm. Combining these three lasers can enable ultrawideband sweeping to improve the axial OCT resolution down to 2 μm. }, keywords = {FDML, Swept source, laser, SS-OCT, OCT, Tunable lasers}, year = {2023}, doi = {10.1117/12.2652884}, URL = {https://doi.org/10.1117/12.2652884} } |
Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 1236805.
DOI: | 10.1117/12.2649963 |
Bibtex: | @inproceedings{10.1117/12.2649963, author = {Paul Strenge and Birgit Lange and Wolfgang Draxinger and Christian Hagel and Christin Grill and Veit Danicke and Dirk Theisen-Kunde and Sonja Spahr-Hess and Matteo M. Bonsanto and Robert Huber and Heinz Handels and Ralf Brinkmann}, title = {{Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography}}, volume = {12368}, booktitle = {Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI}, editor = {Caroline Boudoux and James W. Tunnell}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1236805}, abstract = {The ill-defined tumor borders of glioblastoma multiforme pose a major challenge for the surgeon during tumor resection, since the goal of the tumor resection is the complete removal, while saving as much healthy brain tissue as possible. In recent years, optical coherence tomography (OCT) was successfully used to classify white matter from tumor infiltrated white matter by several research groups. Motivated by these results, a dataset was created, which consisted of sets of corresponding ex vivo OCT images, which were acquired by two OCT-systems with different properties (e.g. wavelength and resolution). Each image was annotated with semantic labels. The labels differentiate between white and gray matter and three different stages of tumor infiltration. The data from both systems not only allowed a comparison of the ability of a system to identify the different tissue types present during the tumor resection, but also enable a multimodal tissue analysis evaluating corresponding OCT images of the two systems simultaneously. A convolutional neural network with dirichlet prior was trained, which allowed to capture the uncertainty of a prediction. The approach increased the sensitivity of identifying tumor infiltration from 58 % to 78 % for data with a low prediction uncertainty compared to a previous monomodal approach. }, keywords = {optical coherence tomography, oct, brain, classification, tumor, dual wavelength, glioblastoma multiforme, tissue analysis}, year = {2023}, doi = {10.1117/12.2649963}, URL = {https://doi.org/10.1117/12.2649963} } |
Accelerating intraoperative tumor histology with sectioning-free multiphoton microscopy, European Journal of Surgical Oncology , vol. 49, no. 2, pp. e210, 02 2023.
DOI: | https://doi.org/10.1016/j.ejso.2022.11.575 |
File: | S0748798322013245 |
Bibtex: | @article{STRAUCH2023e210, title = {Accelerating intraoperative tumor histology with sectioning-free multiphoton microscopy}, journal = {European Journal of Surgical Oncology}, volume = {49}, number = {2}, pages = {e210}, year = {2023}, issn = {0748-7983}, doi = {https://doi.org/10.1016/j.ejso.2022.11.575}, url = {https://www.sciencedirect.com/science/article/pii/S0748798322013245}, author = {Matthias Strauch and Jan Philip Kolb and Christian Rose and Nadine Merg and Jennifer Hundt and Christiane Kümpers and Sven Perner and Sebastian Karpf and Robert Huber} } |
Phase analysis strategies for MHz OCE in the large displacement regime, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 2023. pp. 123670Q.
DOI: | 10.1117/12.2652847 |
Bibtex: | @inproceedings{10.1117/12.2652847, author = {Sazgar Burhan and Nicolas Detrez and Katharina Rewerts and Madita G{\"o}b and Steffen Buschschl{\"u}ter and Christian Hagel and Matteo Mario Bonsanto M.D. and Dirk Theisen-Kunde and Robert Huber and Ralf Brinkmann}, title = {{Phase analysis strategies for MHz OCE in the large displacement regime}}, volume = {12367}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {123670Q}, abstract = {In neurosurgical tumor operations on the central nervous system, intraoperative haptic information often assists for discrimination between healthy and diseased tissue. Thus, it can provide the neurosurgeon with additional intraoperative source of information during resection, next to the visual information by the light microscope, fluorescent dyes and neuronavigation. One approach to obtain elastic and viscoelastic tissue characteristics non-subjectively is phase-sensitive optical coherence elastography (OCE), which is based on the principle of optical coherence tomography (OCT). While phase-sensitive OCE offers significantly higher displacement sensitivity inside a sample than commonly used intensity-based correlation methods, it requires a reliable algorithm to recover the phase signal, which is mathematically restricted in the -π to π range. This problem of phase wrapping is especially critical for inter-frame phase analysis since the time intervals between two referenced voxels is long. Here, we demonstrate a one-dimensional unwrapping algorithm capable of removing up to 4π-ambiguities between two frames in the complex phase data obtained from a 3.2 MHz-OCT system. The high sampling rate allows us to resolve large sample displacements induced by a 200 ms air pulse and acquires pixel-precise detail information. The deformation behavior of the tissue can be monitored over the entire acquisition time, offering various subsequent mechanical analysis procedures. The reliability of the algorithm and imaging concept was initially evaluated using different brain tumor mimicking phantoms. Additionally, results from human ex vivo brain tumor samples are presented and correlated with histological findings supporting the robustness of the algorithm.}, keywords = {Optical Coherence Tomography, Megahertz OCT, Fourier Domain Mode Locking, Optical Coherence Elastography, Phase-sensitive OCT, Phase Unwrapping, Brain tumor, Biomechanics}, year = {2023}, doi = {10.1117/12.2652847}, URL = {https://doi.org/10.1117/12.2652847} } |
2022
Case report: Optical coherence tomography for monitoring biologic therapy in psoriasis and atopic dermatitis, Frontiers in Medicine , vol. 9, 09 2022.
DOI: | 10.3389/fmed.2022.995883 |
File: | fmed.2022.995883 |
Bibtex: | @article{RN5359, author = {Ha-Wissel, L.;Yasak, H.;Huber, R.;Zillikens, D.;Ludwig, R. J.;Thaçi, D. and Hundt, J. E.}, title = {Case report: Optical coherence tomography for monitoring biologic therapy in psoriasis and atopic dermatitis}, journal = {Front Med (Lausanne)}, volume = {9}, pages = {995883}, ISSN = {2296-858X (Print) 2296-858x}, DOI = {10.3389/fmed.2022.995883}, year = {2022}, type = {Journal Article} } |
Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology, Frontiers in Oncology , 08 2022.
DOI: | 10.3389/fonc.2022.896060 |
Bibtex: | @article{Strenge-2022, author = {Strenge, P.;Lange, B.;Grill,C.;Danicke,V.;Theisen-Kunde, D.;Hagel, C.;Spahr-Hess, S.;;Bonsanto, Matteo M.;Handels, H.; and Huber, R.;Brinkmann, R.}, title = {Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology}, journal = {Frontiers in Oncology}, Keywords = {AG-Huber_FDML, AG-Huber_OCT, brain, tumor, glioblastoma multiforme, OCT, neural network, attenuation (absorption) coefficient, optical coherence tomography}, DOI = {https://doi.org/10.3389/fonc.2022.896060}, url = {https://www.frontiersin.org/articles/10.3389/fonc.2022.896060/full}, year = {2022}, type = {Journal Article} } |
Towards phase-stabilized Fourier domain mode-locked frequency combs, Communications Physics , vol. 5, no. 1, 08 2022. Springer Science and Business Media LLC.
DOI: | 10.1038/s42005-022-00960-w |
Bibtex: | @article{Grill2022, doi = {10.1038/s42005-022-00960-w}, year = {2022}, publisher = {Springer Science and Business Media {LLC}}, volume = {{5}}, number = {{1}}, author = {C. Grill, T. Bl\"{o}mker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber}, title = {Towards phase-stabilized Fourier domain mode-locked frequency combs}, journal = {{Communications Physics}}, keywords={AG-Huber_FDML, FDML, Fourier domain mode locking, phase, frequency comb, coherence, beating} } |
Registration of histological brain images onto optical coherence tomography images based on shape information, Physics in Medicine & Biology , 06 2022.
DOI: | 10.1088/1361-6560/ac6d9d |
Bibtex: | @article{Strenge2022, author = {Strenge, P;Lange, B;Grill, C;Draxinger, W;Danicke, V;Theisen-Kunde, D;Hagel, C;Spahr-Hess, S;Bonsanto, Matteo M.;Huber, R;Handels, H and Brinkmann, R}, title = {Registration of histological brain images onto optical coherence tomography images based on shape information}, keywords = {brain, glioblastoma multiforme, shape, OCT, optical coherence tomography, AG-Huber_OCT,}, journal = {Physics in Medicine & Biology}, ISSN = {0031-9155}, url = {http://iopscience.iop.org/article/10.1088/1361-6560/ac6d9d}, year = {2022}, type = {Journal Article} } |
Towards ultra-large area vascular contrast skin imaging using multi-MHz-OCT, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 032022. pp. 27 -- 31.
DOI: | 10.1117/12.2612171 |
Bibtex: | @inproceedings{10.1117/12.2612171, author = {Madita G{\"o}b and Sazgar Burhan and Simon Lotz and Robert Huber}, title = {{Towards ultra-large area vascular contrast skin imaging using multi-MHz-OCT}}, volume = {11948}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1194807}, abstract = {We demonstrate ultra-large field of view OCT scanning using standard optics, a X-Y-galvanometer scanner and a synchronously driven motorized XYZ-positioning stage. The integration of a movable stage into our self-built 3.3 MHz- OCT system allows acquiring coherent ultra-large area images, fully leveraging the high speed potential of our system. For fast OCT-angiography, one galvanometer axis scanner is driven in a repetitive sawtooth pattern, fully synchronized to the movement of the linear stage, to obtain multiple measurements at each position. This technique requires exact synchronization, precise repositioning, and uniform movements with low tolerances to ensure a minimum revisitation error. We analyze error and performance of our setup and demonstrate angiographic imaging.}, keywords = {Optical Coherence Tomography, Fourier Domain Mode Locking, FDML, Optical Coherence Angiography, OCTA, Medical optics and biotechnology, Medical imaging, Three-dimensional image acquisition, Scanners, Microscopy}, year = {2022}, doi = {10.1117/12.2612171}, URL = {https://doi.org/10.1117/12.2612171} } |
Ultra-high-accuracy chromatic dispersion measurement in optical fibers, in Optical Components and Materials XIX , Shibin Jiang and Michel J. F. Digonnet, Eds. SPIE, 032022. pp. 119970L.
DOI: | 10.1117/12.2608773 |
Bibtex: | @inproceedings{10.1117/12.2608773, author = {M. Klufts and S. Lotz and M. Bashir and S. Karpf and R. Huber}, title = {{Ultra-high-accuracy chromatic dispersion measurement in optical fibers}}, volume = {11997}, booktitle = {Optical Components and Materials XIX}, editor = {Shibin Jiang and Michel J. F. Digonnet}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {119970L}, abstract = {The chromatic dispersion in optical fibers is a key property for applications where a broadband light source is used and the timing of each individual wavelength is crucial. Counteracting the timing offset introduced by the fiber is a challenge in many applications especially in mode locked lasers. The dispersion parameters need to be measured with high precision. The length of the fiber, the temperature, and the used wavelength will highly impact the amount of dispersion and the accuracy of the measurement. We developed an ultra-high-accuracy dispersion measurement setup at 1080 ± 50 nm considering all the parameters that may influence the measurement. It is based on a home-built wavelength tunable laser where the output is modulated by an electro-optical modulator connected to a 24 GSamples/s arbitrary waveform generator to a complex pattern consisting of pulses and a 4 GHz sine wave. After passing through the fiber the signal is measured with an 80 GSamples/s real time oscilloscope. The fiber’s temperature is controlled to allow for reproducible measurements over several days and we achieve timing measurement accuracies down to ~200 fs. We also present the performance of the setup at ~850 nm. We will discuss and quantify all effects which can negatively impact the system accuracy and we will report on more cost-effective options using lower performance equipment.}, keywords = {Dispersion measurement, Chromatic dispersion, fiber dispersion measurement, optical component characterization, tunable laser, FDML}, year = {2022}, doi = {10.1117/12.2608773}, URL = {https://doi.org/10.1117/12.2608773} } |
Spectroscopic thermo-elastic optical coherence tomography for tissue characterization, Biomedical Optics Express , vol. 13(3), pp. 1430-1446, 02 2022.
DOI: | 10.1364/BOE.447911 |
Bibtex: | @article{Deen2022, author = {Deen, A D;Van Beusekom, H M. M.;Pfeiffer, T;Stam, M;Kleijn, D De;Wentzel, J;Huber, R;Van Der Steen, A F. W.;Soest, G Van and Wang, T}, title = {Spectroscopic thermo-elastic optical coherence tomography for tissue characterization}, journal = {BioOptExpr}, keywords = {AG-Huber, Endoscopic imaging, Image processing, Image quality, Imaging techniques, Optical imaging, Tissue characterization}, volume = {13(3)}, pages = {1430-1446}, DOI = {10.1364/BOE.447911}, year = {2022}, type = {Journal Article} } |
Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence, Biomed. Opt. Express , vol. 13, no. 2, pp. 713--727, 02 2022. OSA.
DOI: | 10.1364/BOE.448353 |
Bibtex: | @article{Gob:22, author = {Madita G\"{o}b and Tom Pfeiffer and Wolfgang Draxinger and Simon Lotz and Jan Philip Kolb and Robert Huber}, journal = {Biomed. Opt. Express}, keywords = {High speed imaging; Image processing; Image quality; In vivo imaging; Range imaging; Vertical cavity surface emitting lasers}, number = {2}, pages = {713--727}, publisher = {Optica Publishing Group}, title = {Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence}, volume = {13}, month = {Feb}, year = {2022}, url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-13-2-713}, doi = {10.1364/BOE.448353}, abstract = {We present continuous three-dimensional spectral zooming in live 4D-OCT using a home-built FDML based OCT system with 3.28 MHz A-scan rate. Improved coherence characteristics of the FDML laser allow for imaging ranges up to 10 cm. For the axial spectral zoom feature, we switch between high resolution and long imaging range by adjusting the sweep range of our laser. We present a new imaging setup allowing for synchronized adjustments of the imaging range and lateral field of view during live OCT imaging. For this, a novel inline recalibration algorithm was implemented that enables numerical k-linearization of the raw OCT fringes for every frame instead of every volume. This is realized by acquiring recalibration data within the dead time of the raster scan at the turning points of the fast axis scanner. We demonstrate in vivo OCT images of fingers and hands at different resolution modes and show real three-dimensional zooming during live 4D-OCT. A three-dimensional spectral zooming feature for live 4D-OCT is expected to be a useful tool for a wide range of biomedical, scientific and research applications, especially in OCT guided surgery.}, } |
OCT-Guided Surgery for Gliomas: Current Concept and Future Perspectives, Diagnostics , vol. 12, no. 2, pp. 335, 01 2022.
DOI: | 10.3390/diagnostics12020335 |
File: | 335 |
Bibtex: | @article{Yashin-2022, author = {Yashin, K;Bonsanto, M M;Achkasova, K;Zolotova, A;Wael, Al-M;Kiseleva, E;Moiseev, A;Medyanik, I;Kravets, L;Huber, R;Brinkmann, R and Gladkova, N}, title = {OCT-Guided Surgery for Gliomas: Current Concept and Future Perspectives}, journal = {Diagnostics}, volume = {12}, number = {2}, pages = {335}, ISSN = {2075-4418}, keywords = {AG-Huber; optical coherence tomography; brain imaging; neurosurgical guidance; brain tumor; minimally invasive theranostics; intraoperative imaging}, url = {https://www.mdpi.com/2075-4418/12/2/335}, year = {2022}, type = {Journal Article} } |
2021
Comparison of two optical coherence tomography systems to identify human brain tumor, Optical Society of America, Dec.2021. pp. EW1C.7.
DOI: | 10.1117/12.2616044 |
Bibtex: | @inproceedings{Strenge:21, author = {P. Strenge, B. Lange, C. Grill, W. Draxinger, V. Danicke, D. Theisen-Kunde, H. Handels, M. M. Bonsanto, C. Hagel, R. Huber and R. Brinkmann}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT; Absorption coefficient; Attenuation coefficient; Fourier domain mode locking; Multiple scattering; Optical coherence tomography; Spectral domain optical coherence tomography}, pages = {EW1C.7}, publisher = {Optical Society of America}, title = {Comparison of two optical coherence tomography systems to identify human brain tumor}, year = {2021}, url = {https://doi.org/10.1117/12.2616044}, abstract = {The identification of ex vivo brain tumor tissue was investigated with two different optical coherence tomography systems exploiting two optical parameters. The optical parameters were calculated from semantically labelled OCT B-scans.}, } |
Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm2/sec, in European Conferences on Biomedical Optics 2021 (ECBO) , Optical Society of America, Dec.2021. pp. EW3C.4.
DOI: | 10.1117/12.2616054 |
Bibtex: | @inproceedings{Gob:21, author = {Madita G\"{o}b and Sazgar Burhan and Wolfgang Draxinger and Jan Philip Kolb and Robert Huber}, booktitle = {European Conferences on Biomedical Optics 2021 (ECBO)}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT;Fourier domain mode locking; Image processing; Image quality; Optical coherence tomography; Temporal resolution; Three dimensional imaging}, pages = {EW3C.4}, publisher = {Optical Society of America}, title = {Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm$^2$/sec}, year = {2021}, url = {http://www.osapublishing.org/abstract.cfm?URI=ECBO-2021-EW3C.4}, abstract = {We demonstrate a 3.3 MHz A-scan rate OCT for rapid scanning of large areas of human skin. The mosaicking performance and different OCT imaging modalities including intervolume speckle contrast are evaluated.}, } |
Flow Controlled Air Puff Generator Towards In Situ Brain Tumor Detection Based on MHz Optical Coherence Elastography, in ECBO , Optical Society of America, Dec.2021. pp. EW4A.10.
Weblink: | https://opg.optica.org/abstract.cfm?uri=ECBO-2021-EW4A.10 |
Bibtex: | @inproceedings{Detrez:21, author = {N. Detrez, K. Rewerts, M. Matthiae, S. Buschschlueter, M.M. Bonsanto, D. Theisen-Kunde and R. Brinkmann}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT}, pages = {EW4A.10}, publisher = {Optical Society of America}, title = {Flow Controlled Air Puff Generator Towards In Situ Brain Tumor Detection Based on MHz Optical Coherence Elastography}, year = {2021}, url = {https://doi.org/10.1117/12.2615022}, abstract = {A precision air puff excitation system for MHz Optical Coherence Elastography in neurosurgery was developed. It enables non-contact soft-tissue excitation down to {\textmu}N, with direct, noncontact force determination via gas flow measurement.}, } |
Phase-Sensitive Optical Coherence Elastography with a 3.2 MHz FDML-Laser Using Focused Air-Puff Tissue Indentation, in ECBO , Optical Society of America, Dec.2021. pp. ETh3A.3.
Weblink: | https://opg.optica.org/abstract.cfm?URI=ECBO-2021-ETh3A.3 |
Bibtex: | @inproceedings{Rewerts2021ECBO, author = {K. Rewerts, M. Matthiae, N. Detrez, S. Buschschlueter, M.M. Bonsanto, R. Huber and R. Brinkmann}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT}, pages = {ETh3A.3}, publisher = {Optical Society of America}, title = {Phase-Sensitive Optical Coherence Elastography with a 3.2 MHz FDML-Laser Using Focused Air-Puff Tissue Indentation}, year = {2021}, url = {http://www.osapublishing.org/abstract.cfm?URI=ECBO-2021-ETh3A.3}, abstract = {Tumor discrimination from healthy tissue is often performed by haptically probing tissue elasticity. We demonstrate non-contact elastography using air-puff excitation and tissue indentation measurement by phase-sensitive OCT with a 3.2 MHz FDML-laser.}, } |
Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm-1, Optics Letters , vol. 46(14), no. 14, pp. 3456-3459, 07 2021.
DOI: | 10.1364/OL.424726 |
Bibtex: | @article{Hakert2021, author = {H. Hakert, M. Eibl, M. Tillich, R.Pries, G. Hüttmann, R. Brinkmann, B. Wollenberg, K-L. Bruchhage, S. Karpf and R. Huber}, title = {Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800 cm-1}, journal = {Optics Letters}, volume = {46(14)}, number = {14}, pages = {3456-3459}, keywords = {AG-Huber_NL, Clinical applications, Master oscillator power amplifiers, Optical coherence tomography, Raman scattering, Stimulated Raman scattering, Stimulated scattering}, DOI = {https://doi.org/10.1364/OL.424726}, year = {2021}, type = {Journal Article} } |
Imaging Inflammation - From Whole Body Imaging to Cellular Resolution, Frontiers in immunology , vol. 12, pp. 692222-692222, 06 2021.
DOI: | 10.3389/fimmu.2021.692222 |
Bibtex: | @article{PenateMedina2021, author = {Peñate Medina, T;Kolb, J P;Hüttmann, G;Huber, R;Peñate Medina, O;Ha, L;Ulloa, P;Larsen, N;Ferrari, A;Rafecas, M;Ellrichmann, M;Pravdivtseva, M S.;Anikeeva, M;Humbert, J;Both, M;Hundt, J E. and Hövener, J-B}, title = {Imaging Inflammation - From Whole Body Imaging to Cellular Resolution}, journal = {Frontiers in immunology}, keywords = {AG-Huber, MRI, PET, SPECT, optical imaging, Optical coherence tomography (OCT), precision medicine, Two-Photon microscopy (TPM), hyperpolarization}, volume = {12}, pages = {692222-692222}, ISSN = {1664-3224}, DOI = {10.3389/fimmu.2021.692222}, url = {https://pubmed.ncbi.nlm.nih.gov/34248987 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264453/}, year = {2021}, type = {Journal Article} } |
Superposition of two independent FDML lasers, in 2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , 062021. pp. 1-1.
DOI: | 10.1109/CLEO/Europe-EQEC52157.2021.9542126 |
Bibtex: | @INPROCEEDINGS{9542126, author={Grill, Christin and Lotz, Simon and Blömker, Torben and Schmidt, Mark and Draxinger, Wolfgang and Kolb, Jan Philip and Jirauschek, Christian and Huber, Robert}, booktitle={2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)}, title={Superposition of two independent FDML lasers}, year={2021}, volume={}, number={}, pages={1-1}, abstract={Fourier domain mode locking (FDML) is a laser operating regime, which was developed in 2005 [1] . The output of this laser is a train of optical wavelength sweeps, equivalent to extremely chirped pulses with an optical bandwidth of up to 25 THz and frequency tuning rates of >10 19 Hz/s. This laser type was developed for optical coherence tomography [2] , but found recently more and more applications like LiDAR [3] , Raman microscopy [4] or two-photon microscopy [5] . The laser’s coherence properties are relevant for a better understanding of the FDML laser itself and its applications. Because of the wide sweep range and high tuning rate, the laser linewidth cannot be measured with an RF spectrometer. Superposition with a narrowband continuous wave laser only yields phase information for small fractions of the sweep [6] . However, beat signal measurements between two independent FDML lasers with equal sweep range and direction can give information about the complete sweep.}, keywords={}, doi={10.1109/CLEO/Europe-EQEC52157.2021.9542126}, ISSN={}, month={June} } |
High finesse tunable Fabry-Perot filters in Fourier-domain modelocked lasers, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 062021.
DOI: | 10.1117/12.2583501 |
Bibtex: | @inproceedings{Pfeiffer2021, author = {T. Pfeiffer, T. Klein, A. Mlynek, W. Wieser, S. Lotz, C. Grill and R. Huber}, title = {{High finesse tunable Fabry-Perot filters in Fourier-domain modelocked lasers}}, volume = {11630}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, abstract = {We demonstrate that the coherence roll-off and dynamic range of OCT systems using Fourier-domain mode-locked (FDML) lasers can be significantly improved by a fiber Fabry-Perot tunable filter (FFP-TF) with a finesse of more than 3000, a more than fivefold improvement over previous designs. In contrast to previous work, standard resampling using a pre-acquired signal (as in SD-OCT) with no k-clocking is sufficient for 20 nm and 100 nm sweep range, significantly reducing the system complexity. 3D-OCT imaging at 20 cm imaging range is demonstrated.}, keywords = {AG-Huber_FDML, AG-Huber_OCT, optical coherence tomography, FDML laser, swept source laser, high finesse, Fabry-Perot, MHz-OCT, OCT, tunable laser}, year = {2021}, URL = {hhttps://doi.org/10.1117/12.2583501} } |
Ultra-compact tunable fiber laser for coherent anti-Stokes Raman imaging, JRS , 06 2021.
DOI: | 10.1002/jrs.6171 |
Bibtex: | @article{Gottschall2021, author = {T. Gottschall, T. Meyer-Zedler, M. Schmitt, R. Huber, J. Popp, A. Tünnermann and J. Limpert}, title = {Ultra-compact tunable fiber laser for coherent anti-Stokes Raman imaging}, journal = {JRS}, keywords = { AG-Huber_NL, coherent anti-Stokes Raman scattering microscopy, four-wave mixing, nonlinear microscopy, ultrafast laser}, ISSN = {0377-0486}, url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jrs.6171}, year = {2021}, type = {Journal Article} } |
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