Ramtin
Rahmanzadeh,
Prakash
Rai,
Johannes
Gerdes, and
Tayyaba
Hasan,
Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells, Samuel, Achilefu and Ramesh, Raghavachari, Eds. SPIE, 2010. pp. 757602.
Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells, Samuel, Achilefu and Ramesh, Raghavachari, Eds. SPIE, 2010. pp. 757602.
DOI: | 10.1117/12.843850 |
Bibtex: | @inproceedings{Rahmanzadeh, author = {Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba}, title = {Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells}, editor = {Samuel, Achilefu and Ramesh, Raghavachari}, publisher = {SPIE}, volume = {7576}, pages = {757602}, year = {2010}, doi ={10.1117/12.843850}, keywords = {Nanotechnology, Ovarian Cancer, Proliferative Index, Photodynamic Therapy,Antibody} } |
Gereon
Hüttmann,
Cuiping
Yao,
Xiaochao
Qu,
Zhenxi
Zhang, and
Ramtin
Rahmanzadeh,
Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection, J Biomed Opt , vol. 14, pp. 054034, 2009.
Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection, J Biomed Opt , vol. 14, pp. 054034, 2009.
DOI: | 10.1117/1.3253320 |
Bibtex: | @article{Yao, author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.}, title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection}, journal = {J Biomed Opt}, volume = {14}, pages = {054034}, note = {Journal article}, year = {2009} } |
Cuiping
Yao,
Zhenxi
Zhang,
Ramtin
Rahmanzadeh, and
Gereon
Hüttmann,
Laser-based gene transfection and gene therapy, IEEE Trans Nanobioscience , vol. 7, no. 2, pp. 111-9, 2008.
Laser-based gene transfection and gene therapy, IEEE Trans Nanobioscience , vol. 7, no. 2, pp. 111-9, 2008.
DOI: | 10.1109/TNB.2008.2000742 |
Bibtex: | @article{Yao, author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.}, title = {Laser-based gene transfection and gene therapy}, journal = {IEEE Trans Nanobioscience}, volume = {7}, number = {2}, pages = {111-9}, note = {Yao, C P Zhang, Z X Rahmanzadeh, R Huettmann, G Research Support, Non-U.S. Gov't Review United States IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.}, abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.}, keywords = {Cell Membrane/ radiation effects DNA/ administration & dosage/ pharmacokinetics Gene Therapy/ methods Lasers Transfection/ methods}, year = {2008} } |
Jonathan R.
Phillips,
Eberhard
Fischer,
Miriam
Baron,
Niels
Dries,
Fabio
Facchinelli,
Michael
Kutzer,
Ramtin
Rahmanzadeh,
Daniela
Remus, and
Dorothea
Bartels,
Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests, in Plant J , 2008, pp. 938-48.
Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests, in Plant J , 2008, pp. 938-48.
DOI: | 10.1111/j.1365-313X.2008.03478.x |
Bibtex: | @incollection{Phillips, author = {Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.}, title = {Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests}, booktitle = {Plant J}, volume = {54}, edition = {2008/03/19}, pages = {938-48}, note = {Phillips, Jonathan R Fischer, Eberhard Baron, Miriam van den Dries, Niels Facchinelli, Fabio Kutzer, Michael Rahmanzadeh, Ramtin Remus, Daniela Bartels, Dorothea England Plant J. 2008 Jun;54(5):938-48. Epub 2008 Mar 13.}, abstract = {A particular adaptation to survival under limited water availability has been realized in the desiccation-tolerant resurrection plants, which tend to grow in a habitat with seasonal rainfall and long dry periods. One of the best-studied examples is Craterostigma plantagineum. Here we report an unexpected finding: Lindernia brevidens, a close relative of C. plantagineum, exhibits desiccation tolerance, even though it is endemic to the montane rainforests of Tanzania and Kenya, where it never experiences seasonal dry periods. L. brevidens has been found exclusively in two fragments of the ancient Eastern Arc Mountains, which were protected from the devastating Pleistocene droughts by the stable Indian Ocean temperature. Analysis of the microhabitat reveals that L. brevidens is found in the same habitat as hygrophilous plant species, which further indicates that the plant never dries out completely. The objective of this investigation was to address whether C. plantagineum and L. brevidens have desiccation-related pathways in common, or whether L. brevidens has acquired novel pathways. A third, closely related, desiccation-sensitive species, Lindernia subracemosa, has been included for comparison. Mechanisms that confer cellular protection during extreme water loss are well conserved between C. plantagineum and L. brevidens, including the interconversion of 2-octulose to sucrose within the two desiccation-tolerant species. Furthermore, transcriptional control regions of desiccation-related genes belonging to the late embryogenesis abundant (LEA) protein family are also highly conserved. We propose that L. brevidens is a neoendemic species that has retained desiccation tolerance through genome stability, despite tolerance being superfluous to environmental conditions.}, keywords = {Adaptation, Physiological Gene Expression Profiling Genome, Plant Lamiaceae/genetics/metabolism/ physiology Sucrose/metabolism Tropical Climate Water}, year = {2008} } |
Xiaochao
Qu,
Jing
Wang,
Zhenxi
Zhang,
Norbert
Koop,
Ramtin
Rahmanzadeh, and
Gereon
Hüttmann,
Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes, vol. 13, no. 3, pp. 031217, 2008.
Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes, vol. 13, no. 3, pp. 031217, 2008.
DOI: | 10.1117/1.2942373 |
ISBN: | 1083-3668 (Print) 1083-3668 (Linking) |
Bibtex: | @misc{Qu, author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.}, title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes}, volume = {13}, number = {3}, pages = {031217}, note = {Using Smart Source Parsing May-Jun}, abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.}, ISBN = {1083-3668 (Print) 1083-3668 (Linking)}, year = {2008} } |
R.
Rahmanzadeh,
Gereon
Hüttmann,
Johannes
Gerdes, and
Thomas
Scholzen,
Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis, Cell Prolif , vol. 40, no. 3, pp. 422-30, 2007.
Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis, Cell Prolif , vol. 40, no. 3, pp. 422-30, 2007.
DOI: | 10.1111/j.1365-2184.2007.00433.x |
Bibtex: | @article{Rahmanzadeh, author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.}, title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis}, journal = {Cell Prolif}, volume = {40}, number = {3}, pages = {422-30}, note = {Rahmanzadeh, R Huttmann, G Gerdes, J Scholzen, T England Cell Prolif. 2007 Jun;40(3):422-30.}, abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.}, keywords = {Antibodies, Antinuclear Antibodies, Monoclonal Cell Division/physiology Cell Nucleolus/physiology Fluorescein-5-isothiocyanate Fluorescent Dyes HeLa Cells Humans Ki-67 Antigen/*genetics/*metabolism Photochemistry RNA Polymerase I/metabolism RNA, Ribosomal/*biosynthesis}, year = {2007} } |
Cuiping
Yao,
Ramtin
Rahmanzadeh,
Elmar
Endl,
Zhenxi
Zhang,
Johannes
Gerdes, and
Gereon
Hüttmann,
Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles, J Biomed Opt , vol. 10, no. 6, pp. 064012, 2005.
Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles, J Biomed Opt , vol. 10, no. 6, pp. 064012, 2005.
DOI: | http://dx.doi.org/10.1117/1.2137321 |
Bibtex: | @article{Yao, author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.}, title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles}, journal = {J Biomed Opt}, volume = {10}, number = {6}, pages = {064012}, note = {Yao, Cuiping Rahmanzadeh, Ramtin Endl, Elmar Zhang, Zhenxi Gerdes, Johannes Huttmann, Gereon Research Support, Non-U.S. Gov't United States J Biomed Opt. 2005 Nov-Dec;10(6):064012.}, abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.}, keywords = {Biopolymers/pharmacokinetics Cell Line, Tumor Cell Membrane Permeability/ physiology/ radiation effects Drug Delivery Systems/ methods Fluoresceins/ pharmacokinetics Humans Lasers Lymphoma/ metabolism Nanostructures}, year = {2005} } |
Ramtin
Rahmanzadeh,
K.
Muller,
Eberhard
Fischer,
Dorothea
Bartels, and
T.
Borsch,
The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales), vol. 7, no. 1, pp. 67-78, 2005.
The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales), vol. 7, no. 1, pp. 67-78, 2005.
DOI: | 10.1055/s-2004-830444 |
ISBN: | 1435-8603 (Print) 1435-8603 (Linking) |
Bibtex: | @misc{Rahmanzadeh, author = {Rahmanzadeh, R. and Muller, K. and Fischer, E. and Bartels, D. and Borsch, T.}, title = {The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales)}, volume = {7}, number = {1}, pages = {67-78}, month = {Jan}, note = {Rahmanzadeh, R Muller, K Fischer, E Bartels, D Borsch, T Research Support, Non-U.S. Gov't Germany Plant Biol (Stuttg). 2005 Jan;7(1):67-78.}, abstract = {The Lamiales are one of the largest orders of angiosperms, with about 22,000 species. The Scrophulariaceae, as one of their most important families, has recently been shown to be polyphyletic. As a consequence, this family was re-classified and several groups of former scrophulariaceous genera now belong to different families, such as the Calceolariaceae, Plantaginaceae, or Phrymaceae. In the present study, relationships of the genera Craterostigma, Lindernia and its allies, hitherto classified within the Scrophulariaceae, were analyzed. Sequences of the chloroplast trnK intron and the matK gene (approximately 2.5 kb) were generated for representatives of all major lineages of the Lamiales and the former Scrophulariaceae. Bayesian and parsimony analyses revealed two isolated lineages, one of which consists of Lindernia and its allies, the other of Gratiola and allies. Gratiola was previously assumed to be related to Lindernia and was therefore included here. It is proposed to treat the two clades as separate families, Linderniaceae and Gratiolaceae. For the Linderniaceae, several morphological synapomorphies exist in addition to molecular data, such as conspicuous club-shaped stamen appendages.}, keywords = {Bayes Theorem Genes, Plant Introns Phenotype Phylogeny Scrophulariaceae/anatomy & histology/ classification/genetics Species Specificity}, ISBN = {1435-8603 (Print) 1435-8603 (Linking)}, year = {2005} } |