Paula Enzian, and Ramtin Rahmanzadeh,
Photochemical Internalization with Fimaporfin: Enhanced Bleomycin Treatment for Head and Neck Cancer, Pharmaceutics , vol. 15, no. 8, 07 2023.
DOI:10.3390/pharmaceutics15082040
Datei: 2040
Bibtex: BibTeX
@Article{pharmaceutics15082040,
AUTHOR = {Enzian, Paula and Rahmanzadeh, Ramtin},
TITLE = {Photochemical Internalization with Fimaporfin: Enhanced Bleomycin Treatment for Head and Neck Cancer},
JOURNAL = {Pharmaceutics},
VOLUME = {15},
YEAR = {2023},
NUMBER = {8},
ARTICLE-NUMBER = {2040},
URL = {https://www.mdpi.com/1999-4923/15/8/2040},
ISSN = {1999-4923},
ABSTRACT = {Head and neck squamous cell carcinoma (HNSCC) still represents the world’s sixth most common tumor entity, with increasing incidence. The reachability of light makes HNSCC suitable for light-based therapies such as Photochemical Internalization (PCI). The drug Bleomycin is cytotoxic and used as an anti-tumor medication. Since Bleomycin is endocytosed as a relatively large molecule, part of it is degraded in lysosomes before reaching its intracellular target. The goal of our study was to improve the intracellular availability of Bleomycin with PCI. We investigate the intracellular delivery of Bleomycin after PCI with the photosensitizer Fimaporfin. A systematic variation of Bleomycin and Fimaporfin concentrations and light irradiation led to the pronounced cell death of HNSCC cells. After optimization, the same level of tumor cell death of 75% was reached with a 20-fold lower Bleomycin concentration. This would allow treatment of HNSCC with high local tumor cell death and reduce the side effects of Bleomycin, e.g., lung fibrosis, at the same time. This demonstrates the increased efficacy of the anti-tumor medication Bleomycin in combination with PCI.},
DOI = {10.3390/pharmaceutics15082040}
}
Alexander Altmann, Michel Eden, Gereon Hüttmann, Christian Schell, and Ramtin Rahmanzadeh,
Porphyrin-based sensor films for monitoring food spoilage, Food Packaging and Shelf Life , vol. 38, pp. 101105, 06 2023.
DOI:https://doi.org/10.1016/j.fpsl.2023.101105
Datei: S2214289423000820
Bibtex: BibTeX
@article{ALTMANN2023101105,
title = {Porphyrin-based sensor films for monitoring food spoilage},
journal = {Food Packaging and Shelf Life},
volume = {38},
pages = {101105},
year = {2023},
issn = {2214-2894},
doi = {https://doi.org/10.1016/j.fpsl.2023.101105},
url = {https://www.sciencedirect.com/science/article/pii/S2214289423000820},
author = {Alexander Altmann and Michel Eden and Gereon Hüttmann and Christian Schell and Ramtin Rahmanzadeh},
keywords = {Biogenic amines, Sensors, Fish freshness, Food safety, Porphyrin, Smart packaging},
abstract = {To increase food safety and to minimize food waste, it is interesting for the food industry and consumers to be able to determine food spoilage continuously and non-destructively. When food of animal origin is degraded, amines are released as protein breakdown products, which could be used to monitor the freshness of meat and fish. In this work, we introduce a porphyrin-based sensor foil aimed at the detection of biogenic amines. The sensor-porphyrin is formulated on mesoporous silica. Reactivity towards moderate humidity was eliminated by dispersion of the functionalized silica in polyethylene (PE), followed by thermal extrusion resulting in PE foils. After exposure to amines, the sensor foil changes its color irreversibly from green to red. The color change is accompanied by a pronounced shift of the fluorescence spectrum, which was used as a sensitive method to detect the degradation of fish products in model experiments. Titanium dioxide particles in the foil increased the detected fluorescence emission. Experiments with fish filets showed the applicability of the sensor foils in a real-life application by indicating the degree of spoilage after several days, while the microbial growth was depicted by total viable count. We anticipate that our sensor can be an integral part of smart food packages, helping to track the freshness of food during transport or storage.}
}
Alexander Altmann, Mohammad Khodaygani, Martin Leucker, Christian Schell, and Ramtin Rahmanzadeh,
Fluorescence based detection of gaseous food spoilage indicators, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 2023. pp. 126270I.
DOI:10.1117/12.2671751
Datei: 12.2671751
Bibtex: BibTeX
@inproceedings{10.1117/12.2671751,
author = {Alexander Altmann and Mohammad Khodaygani and Martin Leucker and Christian Schell and Ramtin Rahmanzadeh},
title = {{Fluorescence based detection of gaseous food spoilage indicators}},
volume = {12627},
booktitle = {Translational Biophotonics: Diagnostics and Therapeutics III},
editor = {Zhiwei Huang and Lothar D. Lilge},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {126270I},
keywords = {fluorescence spectroscopy, gas sensing, optode technology, food safety, porphyrins, SVM classifier},
year = {2023},
doi = {10.1117/12.2671751},
URL = {https://doi.org/10.1117/12.2671751}
}
Tabea Kohlfaerber, Mario Pieper, Peter König, Ramtin Rahmanzadeh, Gereon Hüttmann, and Hinnerk Schulz-Hildebrandt,
Comparison between dynamic microscopic OCT and autofluorescence multiphoton microscopy for label-free analysis of murine trachea, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , Joseph A Izatt and James G Fujimoto, Eds. SPIE, 2021.
DOI:10.1117/12.2583811
Datei: 12.2583811
Bibtex: BibTeX
@inproceedings{10.1117/12.2583811,

title = {Comparison between dynamic microscopic OCT and autofluorescence multiphoton microscopy for label-free analysis of murine trachea},

author = {Tabea Kohlfaerber and Michael M\"{u}nter and Mario Pieper and Peter K\"{o}nig and Ramtin Rahmanzadeh and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},

editor = {Joseph A Izatt and James G Fujimoto},

url = {https://doi.org/10.1117/12.2583811},

doi = {10.1117/12.2583811},

year  = {2021},

date = {2021-01-01},

booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV},

volume = {11630},

publisher = {SPIE},

organization = {International Society for Optics and Photonics},

keywords = {},

pubstate = {published},

tppubtype = {inproceedings}

}
Cuiping Yao, Florian Rudnitzki, Yida He, Zhenxi Zhang, Gereon Hüttmann, and Ramtin Rahmanzadeh,
Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods, JBio , 2020.
DOI:10.1002/jbio.202000017
Bibtex: BibTeX
@article{Rahmanzadeh-2020,
   author = {Yao, C;Rudnitzki, F;He, Y;Zhang, Z;Hüttmann, G and Rahmanzadeh, R},
   title = {Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods},
   journal = {JBio},
   
   ISSN = {1864-063X},
   DOI = {10.1002/jbio.202000017},
  Year = {2020},
   type = {Journal Article}
}
Michael Münter, Mario Pieper, Malte Casper, Martin Ahrens, Tabea Kohlfaerber, Ramtin Rahmanzadeh, Peter König, Gereon Hüttmann, and Hinnerk Schulz-Hildebrandt,
Dynamic contrast in scanning microscopic OCT, Optic Letters , 2020.
Datei: 2003.00006
Bibtex: BibTeX
@article{Münter2020,
   author = {Münter, M;Endt, M v;Pieper, M;Casper, M;Ahrens, M;Kohlfaerber, T;Rahmanzadeh, R;König, P;Hüttmann, G and Schulz-Hildebrandt, H},
   title = {Dynamic contrast in scanning microscopic OCT},
   journal = {Optic Letters},
url = {https://arxiv.org/abs/2003.00006},
   year = {2020},
   type = {Journal Article}
}
Paula Enzian, Christian Schell, Astrid Link, Carina Malich, Ralph Pries, Barbara Wollenberg, and Ramtin Rahmanzadeh,
Optically controlled drug release from light-sensitive liposomes with the new photosensitizer 5,10-DiOH, Molecular Pharmaceutics , 2020.
DOI:10.1021/acs.molpharmaceut.9b01173
Bibtex: BibTeX
@article{Enzian2020,
   author = {Enzian, P.;Schell, C.;Link, A.;Malich, C.;Pries, R.;Wollenberg, B and Rahmanzadeh, R},
   title = {Optically controlled drug release from light-sensitive liposomes with the new photosensitizer 5,10-DiOH},
   journal = {Molecular Pharmaceutics},
   ISSN = {1543-8384},
   DOI = {10.1021/acs.molpharmaceut.9b01173},
   
   year = {2020},
   type = {Journal Article}
}
Matthias Müller, Ramtin Rahmanzadeh, Thao Tran, Jan Kappelhoff, Eman Aburieda Akam, Peter Caravan, Thomas Jüstel, Kathryn D Held, R.Rox Anderson, and Martin Purschke,
Particle size of X-ray pumped UVC emitting nanoparticles defines intracellular localization and biological activity against cancer cells, Particle and Particle Systems Characterization , 2020.
DOI:10.1002/ppsc.202000201
Datei: 0
Bibtex: BibTeX
@article{Müller2020,
   author = {Müller M;Rahmanzadeh R;Tran T;Kappelhoff J;Akam EA;Caravan P;Jüstel T;Held KD;Anderson R and M, and Purschke},
   title = {Particle size of X-ray pumped UVC emitting nanoparticles defines intracellular localization and biological activity against cancer cells},
   journal = {Particle and Particle Systems Characterization },
   year = {2020},
   type = {Journal Article},
url = { https://onlinelibrary.wiley.com/toc/15214117/0/0}
}
Paula Enzian, Astrid Link, Christian Schell, Carina Malich, and Ramtin Rahmanzadeh,
Light-induced permeabilization of liposomes, vol. 11070, 08 2019. Proc.SPIE.
Datei: 12.2526071
Bibtex: BibTeX
@Proc{Enzian2019,
   author = {Enzian, P.;Link, A.;Schell, C.;Malich, C. and Rahmanzadeh, R.},
   title = {Light-induced permeabilization of liposomes},
   publisher = {Proc.SPIE},
   volume = {11070},
   series = {17th International Photodynamic Association World Congress},
   
   url = {https://doi.org/10.1117/12.2526071},
   year = {2019},
   type = {Book}
}
Tabea Kohlfaerber, Ding Shujun, Ramtin Rahmanzadeh, Thomas Jüngst, Jürgen Groll, Hinnerk Schulz-Hildebrandt, and Gereon Hüttmann,
Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT, Transactions on Additive Manufacturing Meets Medicine 1(1) , 2019.
DOI:10.18416/AMMM.2019.1909S03P19
Bibtex: BibTeX
@article{Kohlfärber2019,
   author = {Kohlfaerber, T;Ding, S;Rahmanzadeh, R;Jüngst, T;Groll, J;Schulz-Hildebrandt, H and Hüttmann, G},
   title = {Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT},
   journal = {Transactions on Additive Manufacturing Meets Medicine 1(1)},
   
   DOI = {10.18416/AMMM.2019.1909S03P19},
   year = {2019},
   type = {Journal Article}
}
Ramtin Rahmanzadeh, Florian Rudnitzki, and Gereon Hüttmann,
Two ways to inactivate the Ki-67 protein—Fragmentation by nanoparticles, crosslinking with fluorescent dyes, Journal of Biophotonics , pp. e201800460, 2019.
DOI:10.1002/jbio.201800460
Datei: jbio.201800460
Bibtex: BibTeX
@article{Rahmanzadeh-2019,
   author = {Rahmanzadeh, R;Rudnitzki, F and Hüttmann, G},
   title = {Two ways to inactivate the Ki-67 protein—Fragmentation by nanoparticles, crosslinking with fluorescent dyes},
   journal = {Journal of Biophotonics},
   Year = {2019},
   pages = {e201800460},
   ISSN = {1864-063X},
   DOI = {10.1002/jbio.201800460},
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201800460},
   type = {Journal Article}
}
Cuiping Yao, Florian Rudnitzki, Gereon Hüttmann, Zhenxi Zhang, and Ramtin Rahmanzadeh,
Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation, International Journal of Nanomedicine , vol. 12, pp. 5659-5672, 2017.
DOI:10.2147/IJN.S140620
Bibtex: BibTeX
@article{Yao2017,
   author = {Yao, C; Rudnitzki, F; Hüttmann, G; Zhang, Zand Rahmanzadeh, R},
   title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
journal = {International Journal of Nanomedicine},
  
   pages = {5659-5672},
   DOI = {10.2147/IJN.S140620},
   year = {2017},
   type = {Journal Article}
}
Sijia Wang, Gereon Hüttmann, Tayyaba Hasan, and Ramtin Rahmanzadeh,
Molecular targeted PDT with selective delivery of ICG Photo-Immunoconjugates(Conference Presentation), in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV , David H. Kessel and Tayyaba Hasan, Eds. SPIE, 042016. pp. 96940O.
DOI:10.1117/12.2217572
Datei: 12.2217572
Bibtex: BibTeX
@inproceedings{10.1117/12.2217572,
author = {Sijia Wang and Gereon H{\"u}ttmann and Tayyaba Hasan and Ramtin Rahmanzadeh},
title = {{Molecular targeted PDT with selective delivery of ICG Photo-Immunoconjugates
(Conference Presentation)}},
volume = {9694},
booktitle = {Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV},
editor = {David H. Kessel and Tayyaba Hasan},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {96940O},
keywords = {photodynamic therapy, liposome, endosomal entrapment, nanotechnology, cell proliferation, photochemical internalization},
year = {2016},
doi = {10.1117/12.2217572},
URL = {https://doi.org/10.1117/12.2217572}
}
Regina Maushagen, Stefan Reers, Ann-Christin Pfannerstill, Angelina Hahlbrock, Roland Stauber, Ramtin Rahmanzadeh, Dirk Rades, Ralph Pries, and Barbara Wollenberg,
Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b, J Cancer Res Clin Oncol , vol. 142, no. 6, pp. 1261--71, 2016.
DOI:10.1007/s00432-016-2150-3
Bibtex: BibTeX
@article{Maushagen2016,
   title        = {Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b},
   author       = {Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.},
   year         = 2016,
   journal      = {J Cancer Res Clin Oncol},
   volume       = 142,
   number       = 6,
   pages        = {1261--71},
   doi          = {10.1007/s00432-016-2150-3},
   issn         = {0171-5216},
   note         = {1432-1335 Maushagen, Regina Reers, Stefan Pfannerstill, Ann-Christin Hahlbrock, Angelina Stauber, Roland Rahmanzadeh, Ramtin Rades, Dirk Pries, Ralph Wollenberg, Barbara Journal Article Germany J Cancer Res Clin Oncol. 2016 Jun;142(6):1261-71. doi: 10.1007/s00432-016-2150-3. Epub 2016 Apr 1.},
   abstract     = {PURPOSE: Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to beta-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet. METHODS: The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies. RESULTS: Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome. CONCLUSION: Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.},
   keywords     = {Apoptosis Caspase 9b Caspases Head and neck cancer Paclitaxel},
   type         = {Journal Article}
}
Sijia Wang, Gereon Hüttmann, Florian Rudnitzki, Heyke Diddens-Tschoeke, Zhenxi Zhang, and Ramtin Rahmanzadeh,
Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy, Journal of Biomedical Optics , vol. 21, no. 7, pp. 078001-078001, 2016.
DOI:10.1117/1.JBO.21.7.078001
Bibtex: BibTeX
@article{Wang2016,
   author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
   title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
   journal = {Journal of Biomedical Optics},
   volume = {21},
   number = {7},
   pages = {078001-078001},
   note = {10.1117/1.JBO.21.7.078001},
   abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
   ISSN = {1083-3668},
   year = {2016},
   type = {Journal Article}
}
Sijia Wang, Gereon Hüttmann, Thomas Scholzen, Zhenxi Zhang, Alfred Vogel, Tayyaba Hasan, and Ramtin Rahmanzadeh,
A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67, Sci Rep , vol. 6, pp. 27032, 2016.
DOI:10.1038/srep27032
Bibtex: BibTeX
@article{Wang2016,
   author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
   title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
   journal = {Sci Rep},
   volume = {6},
   pages = {27032},
   note = {2045-2322
Wang, Sijia
Huttmann, Gereon
Scholzen, Thomas
Zhang, Zhenxi
Vogel, Alfred
Hasan, Tayyaba
Rahmanzadeh, Ramtin
Journal Article
England
Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
   abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
   ISSN = {2045-2322},
   DOI = {10.1038/srep27032},
   year = {2016},
   type = {Journal Article}
}
Sijia Wang, Gereon Hüttmann, Zhenxi Zhang, Alfred Vogel, Reginald Birngruber, Shifalika Tangutoori, Tayyaba Hasan, and Ramtin Rahmanzadeh,
Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67, Mol Pharm , vol. 12, no. 9, pp. 3272-81, 2015.
DOI:10.1021/acs.molpharmaceut.5b00260
Bibtex: BibTeX
@article{Wang2015,
   author = {Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.},
   title = {Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67},
   journal = {Mol Pharm},
   note = {1543-8392
Wang, Sijia
Huttmann, Gereon
Zhang, Zhenxi
Vogel, Alfred
Birngruber, Reginald
Tangutoori, Shifalika
Hasan, Tayyaba
Rahmanzadeh, Ramtin
Journal article
Mol Pharm. 2015 Aug 13.},
   abstract = {The selective inhibition of intracellular and nuclear molecules such as Ki-67 holds great promise for the treatment of cancer and other diseases. However, the choice of the target protein and the intracellular delivery of the functional agent remain crucial challenges. Main hurdles are (a) an effective delivery into cells, (b) endosomal escape of the delivered agents, and (c) an effective, externally triggered destruction of cells. Here we show a light-controlled two-step approach for selective cellular delivery and cell elimination of proliferating cells. Three different cell-penetrating nano constructs, including liposomes, conjugates with the nuclear localization sequence (NLS), and conjugates with the cell penetrating peptide Pep-1, delivered the light activatable antibody conjugate TuBB-9-FITC, which targets the proliferation associated protein Ki-67. HeLa cells were treated with the photosensitizer benzoporphyrin monoacid derivative (BPD) and the antibody constructs. In the first optically controlled step, activation of BPD at 690 nm triggered a controlled endosomal escape of the TuBB-9-FITC constructs. In more than 75% of Ki-67 positive, irradiated cells TuBB-9-FITC antibodies relocated within 24 h from cytoplasmic organelles to the cell nucleus and bound to Ki-67. After a second light irradiation at 490 nm, which activated FITC, cell viability decreased to approximately 13%. Our study shows an effective targeting strategy, which uses light-controlled endosomal escape and the light inactivation of Ki-67 for cell elimination. The fact that liposomal or peptide-assisted delivery give similar results leads to the additional conclusion that an effective mechanism for endosomal escape leaves greater variability for the choice of the delivery agent.},
   keywords = {endosomal entrapment
liposome
nanotechnology
nuclear localization sequence (NLS)
photodynamic therapy},
   ISSN = {1543-8384},
   DOI = {10.1021/acs.molpharmaceut.5b00260},
   year = {2015},
   type = {Journal Article}
}
Florian Rudnitzki, Marco Bever, Ramtin Rahmanzadeh, Katrin Brieger, Elmar Endl, and Jürgen Groll,
Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction, J Biomed Opt , vol. 17, no. 5, pp. 058003, 2012.
DOI:10.1117/1.JBO.17.5.058003
Bibtex: BibTeX
@Article{pmid22612150,
   Author="Rudnitzki, F.  and Bever, M.  and Rahmanzadeh, R.  and Brieger, K.  and Endl, E.  and Groll, J.  and Huttmann, G. ",
   Title="{{B}leaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction}",
   Journal="J Biomed Opt",
   Year="2012",
   Volume="17",
   Number="5",
   Pages="058003",
   Month="May"
}
Ramtin Rahmanzadeh, Jonathan Celli, Imran Rizvi, Johannes Gerdes, and Tayyaba Hasan,
The proliferation marker Ki-67 as novel molecular target for PDT, Photodiagnosis and Photodynamic Therapy , vol. 8, no. 2, pp. 129, 2011.
DOI:10.1016/j.pdpdt.2011.03.025
Bibtex: BibTeX
@article{Rahmanzadeh2011,
   author = {Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.},
   title = {The proliferation marker Ki-67 as novel molecular target for PDT},
   journal = {Photodiagnosis and Photodynamic Therapy},
   volume = {8},
   number = {2},
 pages = {129},
   DOI = {10.1016/j.pdpdt.2011.03.025},
   year = {2011},
   type = {Journal Article}
}
Ramtin Rahmanzadeh, Florian Rudnitzki, Elmar Endl, and Tayyaba Hasan,
Targeted molecular effects through laser-irradiated nanoabsorbers, in Proc. SPIE , Newsrrom, SPIE, Eds. 2011.
DOI:10.1117/2.1201102.003548
Bibtex: BibTeX
@inproceedings{Hüttmann2011,
   author = {Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba},
   title = {Targeted molecular effects through laser-irradiated nanoabsorbers},
   editor = {Newsrrom, SPIE},
   DOI = {10.1117/2.1201102.003548},
   type = {Conference Proceedings},
   year = { 2011}
}
Ramtin Rahmanzadeh, Prakash Rai, Johannes Gerdes, and Tayyaba Hasan,
Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells, Samuel, Achilefu and Ramesh, Raghavachari, Eds. SPIE, 2010. pp. 757602.
DOI:10.1117/12.843850
Bibtex: BibTeX
@inproceedings{Rahmanzadeh,
   author = {Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba},
   title = {Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells},
   editor = {Samuel, Achilefu and Ramesh, Raghavachari},
   publisher = {SPIE},
   volume = {7576},
   pages = {757602},
year = {2010},
doi ={10.1117/12.843850},
keywords = {Nanotechnology, Ovarian Cancer, Proliferative Index, Photodynamic Therapy,Antibody}

}
Ramtin Rahmanzadeh, Prakash Rai, Jonathan Celli, Imran Rizvi, Bettina Baron-Luhr, Johannes Gerdes, and Tayyaba Hasan,
Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer, Cancer Res , vol. 70, no. 22, pp. 9234-42, 2010.
DOI:10.1158/0008-5472.CAN-10-1190
Weblink: https://doi.org/10.1016/j.addr.2010.09.002
Bibtex: BibTeX
@article{Rahmanzadeh2010,
   author = {Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.},
   title = {Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer},
   journal = {Cancer Res},
   volume = {70},
   number = {22},
   pages = {9234-42},
   note = {Using Smart Source Parsing
Nov 15; Epub 2010 Nov 2},
   abstract = {Targeting molecular markers and pathways implicated in cancer cell growth is a promising avenue for developing effective therapies. Although the Ki-67 protein (pKi-67) is a key marker associated with aggressively proliferating cancer cells and poor prognosis, its full potential as a therapeutic target has never before been successfully shown. In this regard, its nuclear localization presents a major hurdle because of the need for intracellular and intranuclear delivery of targeting and therapeutic moieties. Using a liposomally encapsulated construct, we show for the first time the specific delivery of a Ki-67-directed antibody and subsequent light-triggered death in the human ovarian cancer cell line OVCAR-5. Photoimmunoconjugate-encapsulating liposomes (PICEL) were constructed from anti-pKi-67 antibodies conjugated to fluorescein 5(6)-isothiocyanate, as a photoactivatable agent, followed by encapsulation in noncationic liposomes. Nucleolar localization of the PICELs was confirmed by confocal imaging. Photodynamic activation with PICELs specifically killed pKi-67-positive cancer cells both in monolayer and in three-dimensional (3D) cultures of OVCAR-5 cells, with the antibody TuBB-9 targeting a physiologically active form of pKi-67 but not with MIB-1, directed to a different epitope. This is the first demonstration of (a) the exploitation of Ki-67 as a molecular target for therapy and (b) specific delivery of an antibody to the nucleolus in monolayer cancer cells and in an in vitro 3D model system. In view of the ubiquity of pKi-67 in proliferating cells in cancer and the specificity of targeting in 3D multicellular acini, these findings are promising and the approach merits further investigation.},
   year = {2010}
}
Prakash Rai, Srivalleesha Mallidi, Xiang Zheng, Ramtin Rahmanzadeh, Youssef Mir, Stefan Elrington, Ahmat Khurshid, and Tayyaba Hasan,
Development and applications of photo-triggered theranostic agents, Adv Drug Deliv Rev , vol. 62, no. 11, pp. 1094-124, 2010. https://doi.org/10.1016/j.addr.2010.09.002 .
Weblink: https://doi.org/10.1016/j.addr.2010.09.002
Bibtex: BibTeX
@article{Rai,
   author = {Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.},
   title = {Development and applications of photo-triggered theranostic agents},
   journal = {Adv Drug Deliv Rev},
   volume = {62},
   number = {11},
   pages = {1094-124},
   note = {Rai, Prakash
Mallidi, Srivalleesha
Zheng, Xiang
Rahmanzadeh, Ramtin
Mir, Youssef
Elrington, Stefan
Khurshid, Ahmat
Hasan, Tayyaba
Nihms238162
Adv Drug Deliv Rev. 2010 Aug 30;62(11):1094-124. Epub 2010 Sep 19.},
   abstract = {Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.},
   keywords = {Animals
Anti-Infective Agents/diagnostic use/therapeutic use
Antineoplastic Agents/diagnostic use/therapeutic use
Diagnostic Imaging/ methods
Drug Carriers/diagnostic use/therapeutic use
Humans
Infection/ diagnosis/ drug therapy
Nanoparticles/diagnostic use/therapeutic use
Neoplasms/ diagnosis/drug therapy/ therapy
Phototherapy/ methods},
   year = {2010}
}

Gereon Hüttmann, Cuiping Yao, Xiaochao Qu, Zhenxi Zhang, and Ramtin Rahmanzadeh,
Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection, J Biomed Opt , vol. 14, pp. 054034, 2009.
DOI:10.1117/1.3253320
Bibtex: BibTeX
@article{Yao,
   author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
   title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
   journal = {J Biomed Opt},
   volume = {14},
   pages = {054034},
   note = {Journal article},
   year = {2009}
}
Cuiping Yao, Zhenxi Zhang, Ramtin Rahmanzadeh, and Gereon Hüttmann,
Laser-based gene transfection and gene therapy, IEEE Trans Nanobioscience , vol. 7, no. 2, pp. 111-9, 2008.
DOI:10.1109/TNB.2008.2000742
Bibtex: BibTeX
@article{Yao,
   author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.},
   title = {Laser-based gene transfection and gene therapy},
   journal = {IEEE Trans Nanobioscience},
   volume = {7},
   number = {2},
   pages = {111-9},
   note = {Yao, C P
Zhang, Z X
Rahmanzadeh, R
Huettmann, G
Research Support, Non-U.S. Gov't
Review
United States
IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.},
   abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.},
   keywords = {Cell Membrane/ radiation effects
DNA/ administration & dosage/ pharmacokinetics
Gene Therapy/ methods
Lasers
Transfection/ methods},
   year = {2008}
}