Wolfgang Draxinger

Doktorand / PhD Student
Universität zu Lübeck
Institut für Biomedizinische Optik
Maria-Goeppert-Str. 1
23562 Lübeck
Gebäude MFC 1,
Raum 2.24
Email: | wolfgang.draxinger(at)uni-luebeck.de |
Phone: | +49 451 3101 3234 |
Fax: | +49 451 3101 3233 |
2023
Patrick
Kuppler,
Paul
Strenge,
Birgit
Lange,
Sonja
Spahr-Hess,
Wolfgang
Draxinger,
Christian
Hagel,
Dirk
Theisen-Kunde,
Ralf
Brinkmann,
Robert
Huber,
Volker
Tronnier, and
Matteo M.
Bonsanto,
The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study, Frontiers in Oncology , vol. 13, 04 2023.
The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study, Frontiers in Oncology , vol. 13, 04 2023.
DOI: | 10.3389/fonc.2023.1151149 |
Bibtex: | ![]() Kuppler P, Strenge P, Lange B, Spahr-Hess S, Draxinger W, Hagel C, Theisen-Kunde D, Brinkmann R, Huber R, Tronnier V and Bonsanto MM (2023) The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study. Front. Oncol. 13:1151149. doi: 10.3389/fonc.2023.1151149 |
Wolfgang
Draxinger,
Dirk
Theisen-Kunde,
Lion
Schützeck,
Nicolas
Detrez,
Paul
Strenge,
Veit
Danicke,
Jessica
Kren,
Patrick
Kuppler,
Sonja
Spahr-Hess,
Matteo Mario
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time, in High-Speed Biomedical Imaging and Spectroscopy VIII , Kevin K. Tsia and Keisuke Goda, Eds. SPIE, 032023. pp. 123900D.
High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time, in High-Speed Biomedical Imaging and Spectroscopy VIII , Kevin K. Tsia and Keisuke Goda, Eds. SPIE, 032023. pp. 123900D.
DOI: | 10.1117/12.2648505 |
Bibtex: | ![]() @inproceedings{10.1117/12.2648505, author = {Wolfgang Draxinger and Dirk Theisen-Kunde and Lion Sch{\"u}tzeck and Nicolas Detrez and Paul Strenge and Veit Danicke and Jessica Kren and Patrick Kuppler and Sonja Spahr-Hess and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber}, title = {{High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time}}, volume = {12390}, booktitle = {High-Speed Biomedical Imaging and Spectroscopy VIII}, editor = {Kevin K. Tsia and Keisuke Goda}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {123900D}, abstract = {Neuro-surgery is challenged by the difficulties of determining brain tumor boundaries during excisions. Optical coherence tomography is investigated as an imaging modality for providing a viable contrast channel. Our MHz-OCT technology enables rapid volumetric imaging, suitable for surgical workflows. We present a surgical microscope integrated MHz-OCT imaging system, which is used for the collection of in-vivo images of human brains, with the purpose of being used in machine learning systems that shall be trained to identify and classify tumorous tissue.}, keywords = {optical coherence tomography, brain tumor, neurosurgery, machine learning, contrast augmentation, histology dataset, clinical study, in-vivo imaging}, year = {2023}, doi = {10.1117/12.2648505}, URL = {https://doi.org/10.1117/12.2648505} } |
Paul
Strenge,
Birgit
Lange,
Wolfgang
Draxinger,
Christian
Hagel,
Christin
Grill,
Veit
Danicke,
Dirk
Theisen-Kunde,
Sonja
Spahr-Hess,
Matteo M.
Bonsanto,
Robert
Huber,
Heinz
Handels, and
Ralf
Brinkmann,
Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 1236805.
Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 1236805.
DOI: | 10.1117/12.2649963 |
Bibtex: | ![]() @inproceedings{10.1117/12.2649963, author = {Paul Strenge and Birgit Lange and Wolfgang Draxinger and Christian Hagel and Christin Grill and Veit Danicke and Dirk Theisen-Kunde and Sonja Spahr-Hess and Matteo M. Bonsanto and Robert Huber and Heinz Handels and Ralf Brinkmann}, title = {{Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography}}, volume = {12368}, booktitle = {Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI}, editor = {Caroline Boudoux and James W. Tunnell}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1236805}, abstract = {The ill-defined tumor borders of glioblastoma multiforme pose a major challenge for the surgeon during tumor resection, since the goal of the tumor resection is the complete removal, while saving as much healthy brain tissue as possible. In recent years, optical coherence tomography (OCT) was successfully used to classify white matter from tumor infiltrated white matter by several research groups. Motivated by these results, a dataset was created, which consisted of sets of corresponding ex vivo OCT images, which were acquired by two OCT-systems with different properties (e.g. wavelength and resolution). Each image was annotated with semantic labels. The labels differentiate between white and gray matter and three different stages of tumor infiltration. The data from both systems not only allowed a comparison of the ability of a system to identify the different tissue types present during the tumor resection, but also enable a multimodal tissue analysis evaluating corresponding OCT images of the two systems simultaneously. A convolutional neural network with dirichlet prior was trained, which allowed to capture the uncertainty of a prediction. The approach increased the sensitivity of identifying tumor infiltration from 58 % to 78 % for data with a low prediction uncertainty compared to a previous monomodal approach. }, keywords = {optical coherence tomography, oct, brain, classification, tumor, dual wavelength, glioblastoma multiforme, tissue analysis}, year = {2023}, doi = {10.1117/12.2649963}, URL = {https://doi.org/10.1117/12.2649963} } |
2022
Paul
Strenge,
Birgit
Lange,
Wolfgang
Draxinger,
Christin
Grill,
Veit
Danicke,
Dirk
Theisen-Kunde,
Christian
Hagel,
Sonja
Spahr-Hess,
Matteo M.
Bonsanto,
Heinz
Handels,
Ralf
Brinkmann, and
Robert
Huber,
Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology, Frontiers in Oncology , 08 2022.
Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology, Frontiers in Oncology , 08 2022.
DOI: | 10.3389/fonc.2022.896060 |
Bibtex: | ![]() @article{Strenge-2022, author = {Strenge, P.;Lange, B.;Grill,C.;Danicke,V.;Theisen-Kunde, D.;Hagel, C.;Spahr-Hess, S.;;Bonsanto, Matteo M.;Handels, H.; and Huber, R.;Brinkmann, R.}, title = {Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology}, journal = {Frontiers in Oncology}, Keywords = {AG-Huber_FDML, AG-Huber_OCT, brain, tumor, glioblastoma multiforme, OCT, neural network, attenuation (absorption) coefficient, optical coherence tomography}, DOI = {https://doi.org/10.3389/fonc.2022.896060}, url = {https://www.frontiersin.org/articles/10.3389/fonc.2022.896060/full}, year = {2022}, type = {Journal Article} } |
Christin
Grill,
Torben
Blömker,
Mark
Schmidt,
Dominic
Kastner,
Tom
Pfeiffer,
Jan Philip
Kolb,
Wolfgang
Draxinger,
Sebastian
Karpf,
Christian
Jirauschek, and
Robert
Huber,
Towards phase-stabilized Fourier domain mode-locked frequency combs, Communications Physics , vol. 5, no. 1, 08 2022. Springer Science and Business Media LLC.
Towards phase-stabilized Fourier domain mode-locked frequency combs, Communications Physics , vol. 5, no. 1, 08 2022. Springer Science and Business Media LLC.
DOI: | 10.1038/s42005-022-00960-w |
Bibtex: | ![]() @article{Grill2022, doi = {10.1038/s42005-022-00960-w}, year = {2022}, publisher = {Springer Science and Business Media {LLC}}, volume = {{5}}, number = {{1}}, author = {C. Grill, T. Bl\"{o}mker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber}, title = {Towards phase-stabilized Fourier domain mode-locked frequency combs}, journal = {{Communications Physics}}, keywords={AG-Huber_FDML, FDML, Fourier domain mode locking, phase, frequency comb, coherence, beating} } |
Paul
Strenge,
Birgit
Lange,
Christin
Grill,
Wolfgang
Draxinger,
Veit
Danicke,
Dirk
Theisen-Kunde,
Christian
Hagel,
Sonja
Spahr-Hess,
Matteo M.
Bonsanto,
Robert
Huber,
Heinz
Handels, and
Ralf
Brinkmann,
Registration of histological brain images onto optical coherence tomography images based on shape information, Physics in Medicine & Biology , 06 2022.
Registration of histological brain images onto optical coherence tomography images based on shape information, Physics in Medicine & Biology , 06 2022.
DOI: | 10.1088/1361-6560/ac6d9d |
Bibtex: | ![]() @article{Strenge2022, author = {Strenge, P;Lange, B;Grill, C;Draxinger, W;Danicke, V;Theisen-Kunde, D;Hagel, C;Spahr-Hess, S;Bonsanto, Matteo M.;Huber, R;Handels, H and Brinkmann, R}, title = {Registration of histological brain images onto optical coherence tomography images based on shape information}, keywords = {brain, glioblastoma multiforme, shape, OCT, optical coherence tomography, AG-Huber_OCT,}, journal = {Physics in Medicine & Biology}, ISSN = {0031-9155}, url = {http://iopscience.iop.org/article/10.1088/1361-6560/ac6d9d}, year = {2022}, type = {Journal Article} } |
Madita
Göb,
Tom
Pfeiffer,
Wolfgang
Draxinger,
Simon
Lotz,
Jan Philip
Kolb, and
Robert
Huber,
Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence, Biomed. Opt. Express , vol. 13, no. 2, pp. 713--727, 02 2022. OSA.
Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence, Biomed. Opt. Express , vol. 13, no. 2, pp. 713--727, 02 2022. OSA.
DOI: | 10.1364/BOE.448353 |
Bibtex: | ![]() @article{Gob:22, author = {Madita G\"{o}b and Tom Pfeiffer and Wolfgang Draxinger and Simon Lotz and Jan Philip Kolb and Robert Huber}, journal = {Biomed. Opt. Express}, keywords = {High speed imaging; Image processing; Image quality; In vivo imaging; Range imaging; Vertical cavity surface emitting lasers}, number = {2}, pages = {713--727}, publisher = {Optica Publishing Group}, title = {Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence}, volume = {13}, month = {Feb}, year = {2022}, url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-13-2-713}, doi = {10.1364/BOE.448353}, abstract = {We present continuous three-dimensional spectral zooming in live 4D-OCT using a home-built FDML based OCT system with 3.28 MHz A-scan rate. Improved coherence characteristics of the FDML laser allow for imaging ranges up to 10 cm. For the axial spectral zoom feature, we switch between high resolution and long imaging range by adjusting the sweep range of our laser. We present a new imaging setup allowing for synchronized adjustments of the imaging range and lateral field of view during live OCT imaging. For this, a novel inline recalibration algorithm was implemented that enables numerical k-linearization of the raw OCT fringes for every frame instead of every volume. This is realized by acquiring recalibration data within the dead time of the raster scan at the turning points of the fast axis scanner. We demonstrate in vivo OCT images of fingers and hands at different resolution modes and show real three-dimensional zooming during live 4D-OCT. A three-dimensional spectral zooming feature for live 4D-OCT is expected to be a useful tool for a wide range of biomedical, scientific and research applications, especially in OCT guided surgery.}, } |
2021
Paul
Strenge,
Birgit
Lange,
Christin
Grill,
Wolfgang
Draxinger,
Veit
Danicke,
Dirk
Theisen-Kunde,
Heinz
Handels,
Matteo M.
Bonsanto,
Christian
Hagel,
Robert
Huber, and
Ralf
Brinkmann,
Comparison of two optical coherence tomography systems to identify human brain tumor, Optical Society of America, Dez.2021. pp. EW1C.7.
Comparison of two optical coherence tomography systems to identify human brain tumor, Optical Society of America, Dez.2021. pp. EW1C.7.
DOI: | 10.1117/12.2616044 |
Bibtex: | ![]() @inproceedings{Strenge:21, author = {P. Strenge, B. Lange, C. Grill, W. Draxinger, V. Danicke, D. Theisen-Kunde, H. Handels, M. M. Bonsanto, C. Hagel, R. Huber and R. Brinkmann}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT; Absorption coefficient; Attenuation coefficient; Fourier domain mode locking; Multiple scattering; Optical coherence tomography; Spectral domain optical coherence tomography}, pages = {EW1C.7}, publisher = {Optical Society of America}, title = {Comparison of two optical coherence tomography systems to identify human brain tumor}, year = {2021}, url = {https://doi.org/10.1117/12.2616044}, abstract = {The identification of ex vivo brain tumor tissue was investigated with two different optical coherence tomography systems exploiting two optical parameters. The optical parameters were calculated from semantically labelled OCT B-scans.}, } |
Madita
Göb,
Sazgar
Burhan,
Wolfgang
Draxinger,
Jan Philip
Kolb, and
Robert
Huber,
Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm2/sec, in European Conferences on Biomedical Optics 2021 (ECBO) , Optical Society of America, Dez.2021. pp. EW3C.4.
Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm2/sec, in European Conferences on Biomedical Optics 2021 (ECBO) , Optical Society of America, Dez.2021. pp. EW3C.4.
DOI: | 10.1117/12.2616054 |
Bibtex: | ![]() @inproceedings{Gob:21, author = {Madita G\"{o}b and Sazgar Burhan and Wolfgang Draxinger and Jan Philip Kolb and Robert Huber}, booktitle = {European Conferences on Biomedical Optics 2021 (ECBO)}, journal = {European Conferences on Biomedical Optics 2021 (ECBO)}, keywords = {AG-Huber_OCT;Fourier domain mode locking; Image processing; Image quality; Optical coherence tomography; Temporal resolution; Three dimensional imaging}, pages = {EW3C.4}, publisher = {Optical Society of America}, title = {Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm$^2$/sec}, year = {2021}, url = {http://www.osapublishing.org/abstract.cfm?URI=ECBO-2021-EW3C.4}, abstract = {We demonstrate a 3.3 MHz A-scan rate OCT for rapid scanning of large areas of human skin. The mosaicking performance and different OCT imaging modalities including intervolume speckle contrast are evaluated.}, } |
Christin
Grill,
Simon
Lotz,
Torben
Blömker,
Mark
Schmidt,
Wolfgang
Draxinger,
Jan Philip
Kolb,
Christian
Jirauschek, and
Robert
Huber,
Superposition of two independent FDML lasers, in 2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , 062021. pp. 1-1.
Superposition of two independent FDML lasers, in 2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , 062021. pp. 1-1.
DOI: | 10.1109/CLEO/Europe-EQEC52157.2021.9542126 |
Bibtex: | ![]() @INPROCEEDINGS{9542126, author={Grill, Christin and Lotz, Simon and Blömker, Torben and Schmidt, Mark and Draxinger, Wolfgang and Kolb, Jan Philip and Jirauschek, Christian and Huber, Robert}, booktitle={2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)}, title={Superposition of two independent FDML lasers}, year={2021}, volume={}, number={}, pages={1-1}, abstract={Fourier domain mode locking (FDML) is a laser operating regime, which was developed in 2005 [1] . The output of this laser is a train of optical wavelength sweeps, equivalent to extremely chirped pulses with an optical bandwidth of up to 25 THz and frequency tuning rates of >10 19 Hz/s. This laser type was developed for optical coherence tomography [2] , but found recently more and more applications like LiDAR [3] , Raman microscopy [4] or two-photon microscopy [5] . The laser’s coherence properties are relevant for a better understanding of the FDML laser itself and its applications. Because of the wide sweep range and high tuning rate, the laser linewidth cannot be measured with an RF spectrometer. Superposition with a narrowband continuous wave laser only yields phase information for small fractions of the sweep [6] . However, beat signal measurements between two independent FDML lasers with equal sweep range and direction can give information about the complete sweep.}, keywords={}, doi={10.1109/CLEO/Europe-EQEC52157.2021.9542126}, ISSN={}, month={June} } |
Christin
Grill,
Torben
Blömker,
Mark
Schmidt,
Dominic
Kastner,
Tom
Pfeiffer,
Jan Philip
Kolb,
Wolfgang
Draxinger,
Sebastian
Karpf,
Christian
Jirauschek, and
Robert
Huber,
A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 032021. pp. 242 -- 247.
A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 032021. pp. 242 -- 247.
DOI: | 10.1117/12.2578293 |
Bibtex: | ![]() @inproceedings{Grill2021, author = {C. Grill, T. Blömker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber}, title = {{A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements}}, volume = {11665}, booktitle = {Fiber Lasers XVIII: Technology and Systems}, editor = {Michalis N. Zervas}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {242 -- 247}, keywords = {AG-Huber_FDML, Fourier domain mode locking, FDML laser, laser beating , tunable laser, optical coherence tomography, OCT}, year = {2021}, URL = {hhttps://doi.org/10.1117/12.2578293} } |
Paul
Strenge,
Birgit
Lange,
Wolfgang
Draxinger,
Veit
Danicke,
Dirk
Theisen-Kunde,
Heinz
Handels,
Matteo M.
Bonsanto,
Christian
Hagel,
Robert
Huber, and
Ralf
Brinkmann,
Characterization of brain tumor tissue with 1310 nm optical coherence tomography, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 032021. pp. 74 -- 80.
Characterization of brain tumor tissue with 1310 nm optical coherence tomography, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , Joseph A. Izatt and James G. Fujimoto, Eds. SPIE, 032021. pp. 74 -- 80.
DOI: | 10.1117/12.2578409 |
Bibtex: | ![]() @inproceedings{Strenge2021A, author = {P. Strenge, B. Lange, C. Grill, W. Draxinger, V. Danicke, D. Theisen-Kunde, H. Handels, M. Bonsanto, C. Hagel, R. Huber and R. Brinkmann}, title = {{Characterization of brain tumor tissue with 1310 nm optical coherence tomography}}, volume = {11630}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {74 -- 80}, abstract = {The separation of tumorous brain tissue and healthy brain tissue is still a big challenge in the field of neurosurgery, especially when it comes to the detection of different infiltration grades of glioblastoma multiforme at the tumor border. On the basis of a recently created labelled OCT dataset of ex vivo glioblastoma multiforme tumor samples the detection of brain tumor tissue and the identification of zones with varying degrees of infiltration of tumor cells was investigated. The identification was based on the optical properties, which were extracted by an exponential fit function. The results showed that a separation of tumorous tissue and healthy white matter based on these optical properties is possible. A support vector machine was trained on the optical properties to separate tumor from healthy white matter tissue, which achieved a sensitivity of 91% and a specificity of 76% on an independent training dataset.}, keywords = {AG-Huber_OCT, optical coherence tomography, OCT, glioblastoma multiforme, MHz-OCT, brain imaging, tumor, neurosurgery}, year = {2021}, URL = {hhttps://doi.org/10.1117/12.2578409} } |
Paul
Strenge,
Birgit
Lange,
Christin
Grill,
Wolfgang
Draxinger,
Veit
Danicke,
Dirk
Theisen-Kunde,
Heinz
Handels,
Christian
Hagel,
Matteo M.
Bonsanto,
Robert
Huber, and
Ralf
Brinkmann,
Creating a depth-resolved OCT-dataset for supervised classification based on ex vivo human brain samples, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , SPIE, 032021. pp. 66 -- 73.
Creating a depth-resolved OCT-dataset for supervised classification based on ex vivo human brain samples, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV , SPIE, 032021. pp. 66 -- 73.
DOI: | 10.1117/12.2578391 |
Bibtex: | ![]() @inproceedings{Strenge2021, author = {P. Strenge, B. Lange, C. Grill, W. Draxinger, V. Danicke, D. Theisen-Kunde, H. Handels, C. Hagel, M. Bonsanto, R. Huber and R. Brinkmann}, title = {{Creating a depth-resolved OCT-dataset for supervised classification based on ex vivo human brain samples}}, volume = {11630}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {66 -- 73}, abstract = {Optical coherence tomography (OCT) has the potential to become an additional imaging modality for surgical guidance in the field of neurosurgery, especially when it comes to the detection of different infiltration grades of glioblastoma multiforme at the tumor border. Interpretation of the images, however, is still a big challenge. A method to create a labeled OCT dataset based on ex vivo brain samples is introduced. The tissue samples were embedded in an agarose mold giving them a distinctive shape before images were acquired with two OCT systems (spectral domain (SD) and swept source (SS) OCT) and histological sections were created and segmented by a neuropathologist. Based on the given shape, the corresponding OCT images for each histological image can be determined. The transfer of the labels from the histological images onto the OCT images was done with a non-affine image registration approach based on the tissue shape. It was demonstrated that finding OCT images of a tissue sample corresponding to segmented histological images without any color or laser marking is possible. It was also shown that the set labels can be transferred onto OCT images. The accuracy of method is 26 ± 11 pixel, which translates to 192 ± 75 μm for the SS-OCT and 94 ± 43 μm for the SD-OCT. The dataset consists of several hundred labeled OCT images, which can be used to train a classification algorithm.}, keywords = {AG-Huber_OCT, optical coherence tomography, OCT, image registration, glioblastoma multiforme, MHz-OCT, brain imaging, tumor, neurosurgery}, year = {2021}, URL = {https://doi.org/10.1117/12.2578391} } |
Matthias
Strauch,
Jan Philip
Kolb,
Wolfgang
Draxinger,
Ann-Kathrin
Popp,
Melanie
Wacker,
Nadine
Merg,
Jennifer
Hundt,
Sebastian
Karpf, and
Robert
Huber,
Sectioning-free virtual H&E histology with fiber-based two-photon microscopy, in SPIE BiOS , SPIE, 032021.
Sectioning-free virtual H&E histology with fiber-based two-photon microscopy, in SPIE BiOS , SPIE, 032021.
DOI: | 10.1117/12.2578334 |
Bibtex: | ![]() @inproceedings{RN5318, author = {Strauch, M;Kolb, J P;Draxinger, W;Popp, A-K;Wacker, M;Merg, N;Hundt, J;Karpf, S and Huber, R}, title = {Sectioning-free virtual H&E histology with fiber-based two-photon microscopy}, booktitle = {SPIE BiOS}, publisher = {SPIE}, volume = {11648}, Year = {2021}, DOI = {https://doi.org/10.1117/12.2578334}, url = {https://doi.org/10.1117/12.2578334}, type = {Conference Proceedings} } |
Simon
Lotz,
Christin
Grill,
Madita
Göb,
Wolfgang
Draxinger,
Jan Philip
Kolb, and
Robert
Huber,
Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision, Biomedical Optics Express , vol. 12(5), pp. 2604-2616, 03 2021.
Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision, Biomedical Optics Express , vol. 12(5), pp. 2604-2616, 03 2021.
DOI: | 10.1117/12.2578514 |
Bibtex: | ![]() @article{Lotz2021, author = {S. Lotz, C. Grill, M. Göb, W. Draxinger, J.P. Kolb and R. Huber}, title = {Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision}, journal = {Biomedical Optics Express}, volume = {12(5)}, keywords={AG-Huber_FDML}, pages = {2604-2616}, url = {https://doi.org/10.1364/BOE.422898}, year = {2021}, type = {Journal Article} } |
Simon
Lotz,
Christin
Grill,
Madita
Göb,
Wolfgang
Draxinger,
Jan Philip
Kolb, and
Robert
Huber,
Characterization of the dynamics of an FDML laser during closed-loop cavity length control, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 2021. pp. 236 -- 241.
Characterization of the dynamics of an FDML laser during closed-loop cavity length control, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 2021. pp. 236 -- 241.
DOI: | 10.1117/12.2578514 |
Bibtex: | ![]() @inproceedings{LotzLASE2021, author = {S. Lotz, C. Grill, M. Göb, W. Draxinger, J. P. Kolb and R. Huber}, title = {{Characterization of the dynamics of an FDML laser during closed-loop cavity length control}}, volume = {11665}, booktitle = {Fiber Lasers XVIII: Technology and Systems}, editor = {Michalis N. Zervas}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {236 -- 241}, abstract = {In Fourier domain mode locked (FDML) lasers, extremely precise and stable matching of the filter tuning period and light circulation time in the cavity is essential for ultra-low noise operation. During the operation of FDML lasers, the ultra-low noise mode can be lost due to temperature drifts of the already temperature stabilized cavity resulting in increased intensity noise. Until now, the filter frequency is continuously regulated to match the changing light circulation time. However, this causes the filter frequency to constantly change by a few mHz and leads to synchronization issues in cases where a fixed filter frequency is desired. We present an actively cavity length controlled FDML laser and a robust and high precision feedback loop algorithm for maintaining ultra-low noise operation. Instead of adapting the filter frequency, the cavity length is adjusted by a motorized free space beam path to match the fixed filter frequency. The closed-loop system achieves a stability of ~0.18 mHz at a sweep repetition rate of ~418 kHz which corresponds to a ratio of 4×10<sup>-10</sup>. We investigate the coherence properties during the active cavity length adjustments and observe no noise increase compared to fixed cavity length. The cavity length control is fully functional and for the first time, offers the possibility to operate an FDML laser in sweet spot mode at a fixed frequency or phase locked to an external clock. This opens new possibilities for system integration of FDML lasers.}, keywords = {AG-Huber_FDML, FDML, Fourier domain mode locking, laser beating, tunable laser, optical coherence tomography, OCT}, year = {2021}, URL = {hhttps://doi.org/10.1117/12.2578514} } |
2020
Tom
Pfeiffer,
Madita
Göb,
Wolfgang
Draxinger,
Sebastian
Karpf,
Jan Philip
Kolb, and
Robert
Huber,
Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging, Biomed. Opt. Express , vol. 11, no. 11, pp. 6799--6811, Nov. 2020. OSA.
Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging, Biomed. Opt. Express , vol. 11, no. 11, pp. 6799--6811, Nov. 2020. OSA.
DOI: | 10.1364/BOE.402477 |
Bibtex: | ![]() @article{Pfeiffer:20, author = {T. Pfeiffer, M. G\"{o}b, W. Draxinger, S. Karpf, J.P. Kolb and R. Huber}, journal = {Biomed. Opt. Express}, keywords = {AG-Huber_OCT; High speed imaging; Image quality; Optical coherence tomography; Swept lasers; Swept sources; Systems design}, number = {11}, pages = {6799--6811}, publisher = {OSA}, title = {Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging}, volume = {11}, month = {Nov}, year = {2020}, doi = {10.1364/BOE.402477}, abstract = {In order to realize adjustable A-scan rates of fast optical coherence tomography (OCT) systems, we investigate averaging of OCT image data acquired with a MHz-OCT system based on a Fourier Domain Mode Locked (FDML) laser. Increased system sensitivity and image quality can be achieved with the same system at the cost of lower imaging speed. Effectively, the A-scan rate can be reduced in software by a freely selectable factor. We demonstrate a detailed technical layout of the strategies necessary to achieve efficient coherent averaging. Since there are many new challenges specific to coherent averaging in swept source MHz-OCT, we analyze them point by point and describe the appropriate solutions. We prove that coherent averaging is possible at MHz OCT-speed without special interferometer designs or digital phase stabilization. We find, that in our system up to \&\#x223C;100x coherent averaging is possible while achieving a sensitivity increase close to the ideal values. This corresponds to a speed reduction from 3.3 MHz to 33 kHz and a sensitivity gain of 20 dB. We show an imaging comparison between coherent and magnitude averaging of a human finger knuckle joint in vivo with 121\&\#x00A0;dB sensitivity for the coherent case. Further, the benefits of computational downscaling in low sensitivity MHz-OCT systems are analyzed.}, } |
Matthias
Strauch,
Jan Philip
Kolb,
Daniel
Weng,
Melanie
Wacker,
Wolfgang
Draxinger,
Nadine
Merg,
Jennifer
Hundt,
Sebastian
Karpf, and
Robert
Huber,
Two-photon microscopy for sectioning-free virtual H&E imaging, in 104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie , 062020.
Two-photon microscopy for sectioning-free virtual H&E imaging, in 104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie , 062020.
Weblink: | https://www.pathologie-dgp.de/media/Dgp/user_upload/Verhandlungsband_2020_final__kompr._.pdf |
Bibtex: | ![]() @InProceedings{Strauch2020, author = {M. Strauch, J.P. Kolb, D. Weng, M. Wacker, W. Draxinger, N. Merg, J. Hundt, S. Karpf and R. Huber}, booktitle = {104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie}, title = {Two-photon microscopy for sectioning-free virtual {H&E} imaging}, URL = {https://www.pathologie-dgp.de/media/Dgp/user_upload/Verhandlungsband_2020_final__kompr._.pdf}, year = {2020}, keywords = {AG-Huber_NL}, } |
Christin
Grill,
Simon
Lotz,
Torben
Blömker,
Dominic
Kastner,
Tom
Pfeiffer,
Mark
Schmidt,
Wolfgang
Draxinger,
Christian
Jirauschek, and
Robert
Huber,
Beating of two FDML lasers in real time, in Fiber Lasers XVII: Technology and Systems , Liang Dong, Eds. SPIE, 022020. pp. 132 -- 138.
Beating of two FDML lasers in real time, in Fiber Lasers XVII: Technology and Systems , Liang Dong, Eds. SPIE, 022020. pp. 132 -- 138.
DOI: | 10.1117/12.2545794 |
Bibtex: | ![]() @inproceedings{Grill2020, author = {C. {Grill}, S. {Lotz}, T. {Blömker}, D. {Kastner}, T. {Pfeiffer}, S. {Karpf}, M. {Schmidt}, W. {Draxinger}, C. {Jirauschek} and R. {Huber}}, title = {{Beating of two FDML lasers in real time}}, volume = {11260}, booktitle = {Fiber Lasers XVII: Technology and Systems}, editor = {Liang Dong}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {132 -- 138}, keywords = {AG-Huber_FDML, FDML laser, fiber lasers, beat signal, OCT, Optical Coherence Tomography, Fourier domain mode locking}, year = {2020}, doi = {10.1117/12.2545794}, } |
Paul
Strenge,
Birgit
Lange,
Christin
Grill,
Wolfgang
Draxinger,
Matteo M.
Bonsanto,
Christian
Hagel,
Robert
Huber, and
Ralf
Brinkmann,
Segmented OCT data set for depth resolved brain tumor detection validated by histological analysis, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV , SPIE, 022020. pp. 82 -- 89.
Segmented OCT data set for depth resolved brain tumor detection validated by histological analysis, in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV , SPIE, 022020. pp. 82 -- 89.
DOI: | 10.1117/12.2545659 |
Bibtex: | ![]() @inproceedings{Strenge2020, author = {P. Strenge and B. Lange and C. Grill and W. Draxinger and M. M. Bonsanto and C. Hagel and R. Huber and R. Brinkmann}, title = {{Segmented OCT data set for depth resolved brain tumor detection validated by histological analysis}}, volume = {11228}, booktitle = {Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV}, editor = {Joseph A. Izatt and James G. Fujimoto}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {82 -- 89}, keywords = {AG-Huber_OCT, Optical coherence tomography, OCT, FDML Laser, MHz-OCT, brain tumor, brain imaging, neurosurgery}, year = {2020}, URL = { https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11228/112282O/Segmented-OCT-data-set-for-depth-resolved-brain-tumor-detection/10.1117/12.2545659.short} } |
2019
Yoko
Miura,
Wolfgang
Draxinger,
Christin
Grill,
Tom
Pfeiffer,
Salvatore
Grisanti, and
Robert
Huber,
MHz-OCT for low latency virtual reality guided surgery: first wet lab experiments on ex-vivo porcine eye, in Optical Coherence Imaging Techniques and Imaging in Scattering Media III , Maciej Wojtkowski and Stephen A. Boppart and Wang-Yuhl Oh, Eds. SPIE, 072019. pp. 110780E.
MHz-OCT for low latency virtual reality guided surgery: first wet lab experiments on ex-vivo porcine eye, in Optical Coherence Imaging Techniques and Imaging in Scattering Media III , Maciej Wojtkowski and Stephen A. Boppart and Wang-Yuhl Oh, Eds. SPIE, 072019. pp. 110780E.
DOI: | 10.1117/12.2527123 |
Bibtex: | ![]() @inproceedings{10.1117/12.2527123, author = {Yoko Miura and Wolfgang Draxinger and Christin Grill and Tom Pfeiffer and Salvatore Grisanti and Robert Huber}, title = {{MHz-OCT for low latency virtual reality guided surgery: first wet lab experiments on ex-vivo porcine eye }}, volume = {11078}, booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media III}, editor = {Maciej Wojtkowski and Stephen A. Boppart and Wang-Yuhl Oh}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {110780E}, abstract = {MHz-OCT systems based on FDML swept laser sources combined with the massive parallel processing capabilities of modern computer hardware enable volumetric imaging, processing and stereoscopic display at video rates. The increasing image quality and speed might enable new fields of application where the volumetric OCT completely replaces stereoscopic microscopes instead of being a mere supplement. Aside from the depth resolving capability, a particular advantage is the ability to display a whole image volume from arbitrary points of view without the need to move the actual microscope or to rotate the patient’s eye. Purely digital microscopy is already offered as alternative to traditional through-an-eyepiece surgical microscope. We explore the use of virtual reality to present digital OCT microscopy images to a trained surgeon, carrying out a series of surgical procedures ex-vivo on a porcine eye model.}, keywords = {virtual reality, surgery guidance , real-time OCT, user experience}, year = {2019}, doi = {10.1117/12.2527123}, URL = {https://doi.org/10.1117/12.2527123} } |
Julian
Klee,
Jan Philip
Kolb,
Christin
Grill,
Wolfgang
Draxinger,
Tom
Pfeiffer, and
Robert
Huber,
Zero roll-off retinal MHz-OCT using an FDML-laser, in Optical Coherence Imaging Techniques and Imaging in Scattering Media III , SPIE, 072019. pp. 110780S.
Zero roll-off retinal MHz-OCT using an FDML-laser, in Optical Coherence Imaging Techniques and Imaging in Scattering Media III , SPIE, 072019. pp. 110780S.
DOI: | 10.1117/12.2527034 |
Datei: | 12.2527034.short |
Bibtex: | ![]() @inproceedings{10.1117/12.2527034, author = {Julian Klee and Jan Philip Kolb and Christin Grill and Wolfgang Draxinger and Tom Pfeiffer and Robert Huber}, title = {{Zero roll-off retinal MHz-OCT using an FDML-laser}}, volume = {11078}, booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media III}, editor = {Maciej Wojtkowski and Stephen A. Boppart and Wang-Yuhl Oh}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {110780S}, abstract = {Optical coherence tomography (OCT) applications like ultra-widefield and full eye-length imaging are of high interest for various diagnostic purposes. In swept-source OCT these techniques require a swept light source, which is coherent over the whole imaging depth. We present a zero roll-off 1060 nm Fourier Domain Mode Locked-Laser (FDML-Laser) for retinal OCT imaging at 1.7 MHz A-scan rate and first long-range imaging results with it. Several steps such as improved dispersion compensation and frequency regulation were performed and will be discussed. Besides virtually no loss in OCT signal over the maximum depth range of 4.6 mm and very good dynamic range was observed. Roll-off measurements show no decrease of the point-spread function (PSF), while maintaining a high dynamic range.}, keywords = {optical coherence tomography, OCT, tunable laser, Fourier Domain Mode Locking, FDML, MHz OCT}, year = {2019}, doi = {10.1117/12.2527034}, URL = {https://doi.org/10.1117/12.2527034} } |
Jan Philip
Kolb,
Wolfgang
Draxinger,
Julian
Klee,
Tom
Pfeiffer,
Matthias
Eibl,
Thomas
Klein,
Wolfgang
Wieser, and
Robert
Huber,
Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates, PLOS ONE , vol. 14, no. 7, pp. e0213144, 03 2019.
Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates, PLOS ONE , vol. 14, no. 7, pp. e0213144, 03 2019.
DOI: | 10.1371/journal.pone.0213144 |
Bibtex: | ![]() @article{Kolb2019, author = {Kolb, J P;Draxinger, W;Klee, J;Pfeiffer, T;Eibl, M;Klein, T;Wieser, W and Huber, R}, title = {Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates}, journal = {J pone}, keywords = {AG-Huber_OCT}, url = {https://doi.org/10.1371/journal.pone.0213144}, pages = {e0213144}, ISSN = {1932-6203}, year = {2019}, type = {Journal Article} } |
Jan Philip
Kolb,
Daniel
Weng,
Hubertus
Hakert,
Matthias
Eibl,
Wolfgang
Draxinger,
Tobias
Meyer-Zedler,
Thomas
Gottschall,
Ralf
Brinkmann,
Reginald
Birngruber,
Jürgen
Popp,
Jens
Limpert,
Sebastian
Karpf, and
Robert
Huber,
Virtual HE histology by fiber-based picosecond two-photon microscopy, in Multiphoton Microscopy in the Biomedical Sciences XIX , Ammasi Periasamy; Peter T. C. So; Karsten König, Eds. International Society for Optics and Photonics, 022019. pp. 108822F.
Virtual HE histology by fiber-based picosecond two-photon microscopy, in Multiphoton Microscopy in the Biomedical Sciences XIX , Ammasi Periasamy; Peter T. C. So; Karsten König, Eds. International Society for Optics and Photonics, 022019. pp. 108822F.
DOI: | 10.1117/12.2507866 |
Bibtex: | ![]() @inproceedings{10.1117/12.2507866, author = {Jan Philip Kolb and Daniel Weng and Hubertus Hakert and Matthias Eibl and Wolfgang Draxinger and Tobias Meyer and Thomas Gottschall and Ralf Brinkmann and Reginald Birngruber and J{\"u}rgen Popp and Jens Limpert and Sebastian Nino Karpf and Robert Huber}, title = {{Virtual HE histology by fiber-based picosecond two-photon microscopy}}, volume = {10882}, booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX}, editor = {Ammasi Periasamy and Peter T. C. So and Karsten K{\"o}nig}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {108822F}, abstract = {Two-Photon Microscopy (TPM) can provide three-dimensional morphological and functional contrast in vivo. Through proper staining, TPM can be utilized to create virtual, HE equivalent images and thus can improve throughput in histology-based applications. We previously reported on a new light source for TPM that employs a compact and robust fiber-amplified, directly modulated laser. This laser is pulse-to-pulse wavelength switchable between 1064 nm, 1122 nm, and 1186 nm with an adjustable pulse duration from 50ps to 5ns and arbitrary repetition rates up to 1MHz at kW-peak powers. Despite the longer pulse duration, it can achieve similar average signal levels compared to fs-setups by lowering the repetition rate to achieve similar cw and peak power levels. The longer pulses lead to a larger number of photons per pulse, which yields single shot fluorescence lifetime measurements (FLIM) by applying a fast 4 GSamples/s digitizer. In the previous setup, the wavelengths were limited to 1064 nm and longer. Here, we use four wave mixing in a non-linear photonic crystal fiber to expand the wavelength range down to 940 nm. This wavelength is highly suitable for imaging green fluorescent proteins in neurosciences and stains such as acridine orange (AO), eosin yellow (EY) and sulforhodamine 101 (SR101) used for histology applications. In a more compact setup, we also show virtual HE histological imaging using a direct 1030 nm fiber MOPA.}, keywords = {Multiphoton Microscopy, Four Wave Mixing, FWM, Histology, Laser, Non Linear Microscopy, Two Photon Microscopy, JenLab Young Investigator Award}, year = {2019}, doi = {10.1117/12.2507866}, URL = {https://doi.org/10.1117/12.2507866} } |