Publikationen AG-Hüttmann

DOI Link

2018

  • Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Gereon Hüttmann and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten: Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation. Journal of Medical Robotics Research, no. 3, pp. 184005, 2018
    BibTeX Link Link
    @article{borghesan2018single,
    title = {Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation},
    author = {Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten},
    editor = {World Scientific},
    url = {https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    doi = {10.1142/S2424905X18400056},
    year = {2018},
    date = {2018-02-12},
    journal = {Journal of Medical Robotics Research},
    volume = {3},
    number = {02},
    pages = {184005},
    abstract = {Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures or to inadvertently injure the underlying retina. Damage to the latter is mostly irreparable and might cause permanent loss of vision. Despite the availability over excellent stereo microscopes, wide-angle lenses and powerful light source visualization remains a problem. Especially, the limited depth perception is still perceived as a major bottle-neck whereas efforts have been conducted to integrate sensing capability in today’s state-of-the-art instruments, so far, little effort has been paid to process the obtained sensor data and turns this into a reliable source of information upon which robot assistive guidance schemes could be endowed upon. This paper proposes a number of processing techniques tailored to Optical Coherence Tomography (OCT) measurements. The first results of the proposed algorithms show that it is feasible to extract good and reliable distance estimates from this otherwise rather noisy signal and from a fairly limited dataset. The used data are the so-called A-scans. These are OCT measurements consisting of a single-line image that could be captured by an instrument-mounted fiber through which the OCT signal passes back-and-forth. However, in this work, we perform a pilot study whereby the employed A-scans are extracted from B-scans that are captured by a microscope-mounted OCT scanner, rather than obtained from a probe. The performance of a first embodiment of the algorithm that is based on an Unscented Kalman Filter (UKF) is compared to the performance of a second embodiment that relies on a Particle Filter (PF), focusing on the issues in filter initialization and the tracking quality. Finally, results of UKF and PF executions on a validation dataset are presented. 
    Read More: https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Sudkamp, Helge and Hillmann, Dierck and Koch, Peter and Endt, Malte vom and Spahr, Hendrik and Münst, Michael and Pfäffle, Clara and Birngruber, Reginald and Hüttmann, Gereon: Simple approach for aberration-corrected OCT imaging of the human retina. Optics Letters, no. 43, pp. 4224, 2018
    BibTeX Link
    @article{Sudkamp2018a,
       author = {Sudkamp, Helge and Hillmann, Dierck and Koch, Peter and Endt, Malte vom and Spahr, Hendrik and Münst, Michael and Pfäffle, Clara and Birngruber, Reginald and Hüttmann, Gereon},
       title = {Simple approach for aberration-corrected OCT imaging of the human retina},
       journal = {Optics Letters},
       volume = {43},
       number = {17},
       pages = {4224},
       ISSN = {0146-9592
    1539-4794},
       DOI = {10.1364/ol.43.004224},
       year = {2018},
       type = {Journal Article},
       keyword = {Retome}
    }
    
  • Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Reginald Birngruber and Dieter Manstein and Gereon Hüttmann: Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation). Proc.SPIE, in Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, no. 10467, 2018
    BibTeX Link Link
    @conference{Casper2018,
    title = {Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation)},
    author = {Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Reginald Birngruber and Dieter Manstein and Gereon H\"{u}ttmann},
    url = {https://doi.org/10.1117/12.2292005},
    doi = {10.1117/12.2292005},
    year = {2018},
    date = {2018-03-14},
    booktitle = {Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018},
    journal = {Proc.SPIE},
    volume = {10467},
    abstract = {The ability to image the physiology of microvasculature with high spatial resolution in three dimensions while investigating structural changes of skin, is essential for understanding the complex processes of skin aging, wound healing and disease development. Further, the quantitative, automatic assessment of these changes enables to analyze large amounts of image data in an abstract but comprehensive manner. 
    However, previous work using OCT with methods of angiography was imaging less scattering, hence more challenging tissue than skin, such as brain and retina tissue. The published methods for capillary segmentation were mostly non-automatic, poorly benchmarked against state-of-the-art methods of computer vision and not applied to investigate medical processes and studies in a comprehensive fashion. 
    Here, segmentation of capillaries in skin is reported and its efficacy is demonstrated in both, a 
    longitudinal mouse study and a preliminary study in humans. By combining state-of-the-art image 
    processing methods in an optimized way, we were able to improve the segmentation results and analyze the impact of each post-processing step. 
    Furthermore, this automatic segmentation enabled us to analyze big amounts of 
    datasets automatically and derive meaningful conclusions for the planning of clinical studies. 
    With this work, optical coherence tomography is combined with methods of computer vision to a diagnostic 
    tool with unique capabilities to characterize vascular diversity and provide extraordinary 
    opportunities for dermatological investigation in both, clinics and research.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Hinnerk Schulz-Hildebrandt and Mario Pieper and Charlotte Stehmar and Martin Ahrens and Christian Idel and Barbara Wollenberg and Peter König and Gereon Hüttmann: Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography. Biomedical Optics Express, no. 9, pp. 636-647, 2018
    BibTeX Link Link
    @article{schulz2018novel,
    title = {Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography},
    author = {Hinnerk Schulz-Hildebrandt and Mario Pieper and Charlotte Stehmar and Martin Ahrens and Christian Idel and Barbara Wollenberg and Peter K\"{o}nig and Gereon H\"{u}ttmann},
    editor = {Optical Society of America},
    url = {https://www.osapublishing.org/abstract.cfm?URI=boe-9-2-636
    https://www.osapublishing.org/boe/viewmedia.cfm?uri=boe-9-2-636&seq=0},
    doi = {10.1364/BOE.9.000636},
    isbn = {10.1364/BOE.9.000636},
    year = {2018},
    date = {2018-01-16},
    journal = {Biomedical Optics Express},
    volume = {9},
    number = {2},
    pages = {636-647},
    abstract = {Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 \textendash 2 μm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests overall a better image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 μm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was determined successfully. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.},
    keywords = {Endoskope, OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Hendrik Spahr and Clara Pfäffle and Peter Koch and Helge Sudkamp and Gereon Hüttmann and Dierck Hillmann: Interferometric detection of 3D motion using computational subapertures in optical coherence tomography. Opt. Express, no. 26, pp. 18803-18816, OSA, Jul, 2018
    BibTeX Link Link
    @article{Spahr:18,
    author = {Hendrik Spahr, Clara Pfäffle, Peter Koch, Helge Sudkamp, Gereon Hüttmann und Dierck Hillmann},
    journal = {Opt. Express},
    keywords = {Funktion, Fullfield},
    number = {15},
    pages = {18803--18816},
    publisher = {OSA},
    title = {Interferometric detection of 3D motion using computational subapertures in optical coherence tomography},
    volume = {26},
    month = {Jul},
    year = {2018},
    url = {http://www.opticsexpress.org/abstract.cfm?URI=oe-26-15-18803},
    doi = {10.1364/OE.26.018803},
    abstract = {Doppler optical coherence tomography (OCT) quantifies axial motion with high precision, whereas lateral motion cannot be detected by a mere evaluation of phase changes. This problem was solved by the introduction of three-beam Doppler OCT, which, however, entails a high experimental effort. Here, we present the numerical analogue to this experimental approach. Phase-stable complex-valued OCT datasets, recorded with full-field swept-source OCT, are filtered in the Fourier domain to limit imaging to different computational subapertures. These are used to calculate all three components of the motion vector with interferometric precision. As known from conventional Doppler OCT for axial motion only, the achievable accuracy exceeds the actual imaging resolution by orders of magnitude in all three dimensions. The feasibility of this method is first demonstrated by quantifying micro-rotation of a scattering sample. Subsequently, a potential application is explored by recording the 3D motion vector field of tissue during laser photocoagulation in ex-vivo porcine retina.},
    }
    
    
  • Hinnerk Schulz-Hildebrandt /Tom Pfeiffer /Tim Eixmann /Sabrina Lohmann /Martin Ahrens /Josua Rehra /Wolfgang Draxinger /Peter König /Robert Huber and Gereon Hüttmann: High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography. Optics Letters, no. 43, pp. 4386-4389, 2018
    BibTeX Link Link
    @article{Schulz-Hildebrandt2018b,
    title = {High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography},
    author = {Hinnerk Schulz-Hildebrandt /Tom Pfeiffer /Tim Eixmann /Sabrina Lohmann /Martin Ahrens /Josua Rehra /Wolfgang Draxinger /Peter K\"{o}nig /Robert Huber and Gereon H\"{u}ttmann},
    url = {ttp://ol.osa.org/abstract.cfm?URI=ol-43-18-4386},
     DOI = {10.1364/OL.43.004386},
    
    year = {2018},
    date = {2018-09-05},
    journal = {Optics Letters},
    volume = {43},
    number = {18},
    pages = {4386-4389},
    abstract = {We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 μm was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in spirals for scanning the sample, the beam quality remains constant over the entire field of view with a diameter of 0.8 mm. The scanning frequency was adjusted to 1.22 kHz for use with a 3.28 MHz Fourier domain mode locked OCT system. Densely sampled volumes have been imaged at a rate of 6 volumes per second.
    
    © 2018 Optical Society of America},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Jonas Smits and Mouloud Ourak and Andy Gijbels and Laure Esteveny and Gianni Borghesan and Laurent Schoevaerdts and K. Willekens P. Stalmans E. Lankenau Hinnerk Schulz-Hildebrandt and Gereon Hüttmann and Dominiek Reynaerts and Emmanuel B. Vander Poorten: Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation. 2018 IEEE International Conference on Robotics and Automation (ICRA), pp. 129-134, 2018
    BibTeX Link Link
    @inproceedings{Smits2018,
    title = {Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation},
    author = {Jonas Smits and Mouloud Ourak and Andy Gijbels and Laure Esteveny and Gianni Borghesan and Laurent Schoevaerdts and K. Willekens P. Stalmans E. Lankenau Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Dominiek Reynaerts and Emmanuel B. Vander Poorten},
    
    doi = {10.1109/ICRA.2018.8460983},
    year = {2018},
    date = {2018-09-20},
    journal = {2018 IEEE International Conference on Robotics and Automation (ICRA)},
    pages = {129-134},
    abstract = {Retinal Vein Occlusion is a common retinal vascular disorder which can cause severe loss of vision. Retinal vein cannulation followed by injection of an anti-coagulant into the affected vein is a promising treatment. However, given the scale and fragility of the surgical workfield, this procedure is considered too high-risk to perform manually. A first successful robot-assisted procedure has been demonstrated. Even though successful, the procedure remains extremely challenging. This paper aims at providing a solution for the limited perception of instrument-tissue interaction forces as well as depth estimation during retinal vein cannulation. The development of a novel combined force and distance sensing cannulation needle relying on Fiber Bragg grating (FBG) and Optical Coherence Tomography (OCT) A-scan technology is reported. The design, the manufacturing process, the calibration method, and the experimental characterization of the produced sensor are discussed. 
    The functionality of the combined sensing modalities and the real-time distance estimation algorithm are validated respectively on in-vitro and ex-vivo models.},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Ourak, M. and Smits, J. and Esteveny, L. and Borghesan, G. and Gijbels, A. and Schoevaerdts, L. and Douven, Y. and Scholtes, J. and Lankenau, E. and Eixmann, T. and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and Kozlovszky, M. and Kronreif, G. and Willekens, K. and Stalmans, P. and Faridpooya, K. and Cereda, M. and Giani, A. and Staurenghi, G. and Reynaerts, D. and Vander Poorten, E. B.: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. International Journal of Computer Assisted Radiology and Surgery, no. 13, pp. 1-9, 2018
    BibTeX Link Link
    @article{Ourak2018,
    title = {Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation},
    author = {Ourak, M. and Smits, J. and Esteveny, L. and Borghesan, G. and Gijbels, A. and Schoevaerdts, L. and Douven, Y. and Scholtes, J. and Lankenau, E. and Eixmann, T. and Schulz-Hildebrandt, Hinnerk and H\"{u}ttmann, Gereon and Kozlovszky, M. and Kronreif, G. and Willekens, K. and Stalmans, P. and Faridpooya, K. and Cereda, M. and Giani, A. and Staurenghi, G. and Reynaerts, D. and Vander Poorten, E. B.},
    
    doi = {10.1007/s11548-018-1829-0},
    year = {2018},
    date = {2018-07-28},
    journal = {International Journal of Computer Assisted Radiology and Surgery},
    volume = {13},
    number = {107},
    pages = {1-9},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Hinnerk Schulz-Hildebrandt and Michael Münter and Martin Ahrens and Hendrik Spahr and Dierck Hillmann and Peter König and Gereon Hüttmann: Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations. in 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources, no. 10591, pp. 105910O, 2018
    BibTeX Link
    @inproceedings{Schulz-Hildebrandt2018a,
    title = {Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations},
    author = {Hinnerk Schulz-Hildebrandt and Michael M\"{u}nter and Martin Ahrens and Hendrik Spahr and Dierck Hillmann and Peter K\"{o}nig and Gereon H\"{u}ttmann},
    doi = {10.1117/12.2303755},
    isbn = {9781510616745},
    year = {2018},
    date = {2018-03-05},
    booktitle = {2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources},
    volume = {10591},
    pages = {105910O},
    abstract = {Optical coherence tomography (OCT) images scattering tissues with 5 to 15 μm resolution. This is usually not sufficient for a distinction of cellular and subcellular structures. Increasing axial and lateral resolution and compensation of artifacts caused by dispersion and aberrations is required to achieve cellular and subcellular resolution. This includes defocus which limit the usable depth of field at high lateral resolution. OCT gives access the phase of the scattered light and hence correction of dispersion and aberrations is possible by numerical algorithms. Here we present a unified dispersion/aberration correction which is based on a polynomial parameterization of the phase error and an optimization of the image quality using Shannon’s entropy. For validation, a supercontinuum light sources and a costume-made spectrometer with 400 nm bandwidth were combined with a high NA microscope objective in a setup for tissue and small animal imaging. Using this setup and computation corrections, volumetric imaging at 1.5 μm resolution is possible. Cellular and near cellular resolution is demonstrated in porcine cornea and the drosophila larva, when computational correction of dispersion and aberrations is used. Due to the excellent correction of the used microscope objective, defocus was the main contribution to the aberrations. In addition, higher aberrations caused by the sample itself were successfully corrected. Dispersion and aberrations are closely related artifacts in microscopic OCT imaging. Hence they can be corrected in the same way by optimization of the image quality. This way microscopic resolution is easily achieved in OCT imaging of static biological tissues.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {inproceedings}
    }

2017

  • Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon: Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]. Biomedical Optics Express, no. 8, pp. 1499-1511, 2017
    BibTeX Link
    @article{Pfäffle2017,
       author = {Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon},
       title = {Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {3},
       pages = {1499-1511},
       keywords = {Image reconstruction-restoration
    Optical coherence tomography},
       url = {http://www.osapublishing.org/boe/abstract.cfm?URI=boe-8-3-1499},
       year = {2017},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon: Off-axis reference beam for full-field swept-source OCT and holoscopy. Optics Express, no. 25, pp. 27770-27784, 2017
    BibTeX Link
    @article{Hillmann2017,
       author = {Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon},
       title = {Off-axis reference beam for full-field swept-source OCT and holoscopy},
       journal = {Optics Express},
       volume = {25},
       number = {22},
       pages = {27770-27784},
       DOI = {10.1364/OE.25.027770},
       year = {2017},
       type = {Journal Article}
    }
    
  • Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.: Noncontact holographic detection for photoacoustic tomography. J Biomed Opt, no. 22, pp. 1-14, 2017
    BibTeX Link
    @article{Buj2017,
       author = {Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.},
       title = {Noncontact holographic detection for photoacoustic tomography},
       journal = {J Biomed Opt},
       volume = {22},
       number = {10},
       pages = {1-14},
       DOI = {10.1117/1.jbo.22.10.106007},
       year = {2017},
       type = {Journal Article}
    }
    
    
    
  • Jens Horstmann and Hinnerk Schulz-Hildebrandt and Felix Bock and Sebastian Siebelmann and Eva Lankenau and Gereon Hüttmann and Philipp Steven and Claus Cursiefen: Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography. Investigative Ophthalmology & Visual Science, no. 58, pp. 5880-5886, 2017
    BibTeX Link Link
    @article{Horstmann2017,
    title = {Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography},
    author = {Jens Horstmann and Hinnerk Schulz-Hildebrandt and Felix Bock and Sebastian Siebelmann and Eva Lankenau and Gereon H\"{u}ttmann and Philipp Steven and Claus Cursiefen},
    editor = {The Association Research for in Vision and Ophthalmology},
    url = {http://iovs.arvojournals.org/article.aspx?articleid=2664271},
    doi = {10.1167/iovs.17-22286},
    isbn = {1552-5783},
    year = {2017},
    date = {2017-12-25},
    journal = {Investigative Ophthalmology & Visual Science},
    volume = {58},
    number = {13},
    pages = {5880-5886},
    abstract = {Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). 
    
    Methods: Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were acquired using a custom-built mOCT system enabling a resolution of 1 μm at a B-scan rate of 165/s. Three of the six animals received an additional intrastromal injection of India ink 24 hours before the measurement to stain the corneal lymphatic system in vivo. Immunohistochemistry using CD31 and LYVE-1 was used to validate the mOCT findings. 
    
    Results: Using mOCT, lymphatic vessels were visible as dark vessel-like structures with the lumen lacking a hyperreflective wall and mostly lacking cells. However, individual, slowly moving particles, which most likely are immune cells, occasionally could be observed inside the lumen. In lymphatic vessels of ink-stained corneas, hyperreflection and shadowing underneath was observed. Ink-filled lymphatic vessels were colocalized in consecutive corneal flat mounts of the same specimen. 
    
    Conclusions: Corneal lymphatic vessels can be imaged using mOCT. This novel approach opens new options for noninvasive clinical imaging of corneal lymphatic vessels for diagnostic and therapeutic indications.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation. International Journal of Nanomedicine, no. Volume 12, pp. 5659-5672, 2017
    BibTeX Link
    @article{Yao2017,
       author = {Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
    journal = {International Journal of Nanomedicine},
       volume = {Volume 12},
       pages = {5659-5672},
       DOI = {10.2147/IJN.S140620},
       year = {2017},
       type = {Journal Article}
    }
    
  • Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.: Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea. Am J Pathol, no. 187, pp. 2451-2460, 2017
    BibTeX Link
    @article{Kretschmer2017,
       author = {Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.},
       title = {Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea},
       journal = {Am J Pathol},
       ISSN = {0002-9440},
       DOI = {10.1016/j.ajpath.2017.07.006},
      volume = {187},
      number = {11},
       year = {2017},
    pages = {2451-2460},
       type = {Journal Article}
    }
    
  • Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon Hüttmann and Dieter Manstein: Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation). in Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery, no. 10037OS, 2017
    BibTeX Link
    @conference{Casper2017,
    title = {Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)},
    author = {Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon H\"{u}ttmann and Dieter Manstein},
    editor = {Spie},
    doi = {doi: 10.1117/12.2251485},
    year = {2017},
    date = {2017-04-19},
    booktitle = {Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery},
    volume = {10037OS},
    abstract = {In dermatology the reflexes of vasoconstriction and vasodilation are known as important mechanisms of thermoregulation of the inner body. Imaging the physiology of microvasculature of the skin with high spatial resolution in three dimensions while reacting to changes in temperature is crucial for understanding the complex processes of vasodynamics, which result in constriction and dilation of vessels. However, previous studies using Laser-Doppler flowmetry and -imaging could not provide reliable angiographic images which allow to quantify changes in blood vessel diameter. Here, we report a different approach for angiographic imaging of microvasculature of a anaesthetized rodent model using speckle variance optical coherence tomography (svOCT) during and after localized cooling. Therefore a commercial OCT with a center wavelength of 1.3 μm and a spatial resolution of 13µm was used in combination with a custom built cooling device to image such reflexes at the mouse ear pinna and dorsal skinfold. Cooling was applied in steps of 2−5◦ C starting at the baseline temperature of 27◦ C down to −10◦ C. To our surprise and in contrast to the general opinion in literature, we were able to observe that the majority of vessels with a diameter larger than 20 μm maintain perfused with a constant diameter when the tissue is cooled from baseline to subzero temperatures. However, vasoconstriction was observed very rarely and only in veins, which led to their occlusion. The results of this experiment lead us to reconsider essential aspects of previous understanding of temperature-induced vasodynamics in cutaneous microvasculature.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Sarah Latus and Christoph Otte and Matthias Schlüter and Josua Rehra and Kevin Bizon and Hinnerk Schulz-Hildebrandt and Thore Saathoff and Gereon Hüttmann and Alexander Schlaefer: An Approach for Needle Based Optical Coherence Elastography Measurements. in Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017, no. 10434, pp. 655-663, 2017
    BibTeX Link Link
    @conference{Latus2017,
    title = {An Approach for Needle Based Optical Coherence Elastography Measurements},
    author = {Sarah Latus and Christoph Otte and Matthias Schl\"{u}ter and Josua Rehra and Kevin Bizon and Hinnerk Schulz-Hildebrandt and Thore Saathoff and Gereon H\"{u}ttmann and Alexander Schlaefer},
    editor = {Springer International Publishing},
    url = {https://link.springer.com/chapter/10.1007/978-3-319-66185-8_74},
    doi = {https://doi.org/10.1007/978-3-319-66185-8_74},
    isbn = {978-3-319-66185-8},
    year = {2017},
    date = {2017-09-01},
    booktitle = {Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017},
    volume = {10434},
    pages = {655--663},
    organization = {Springer},
    abstract = {© Springer International Publishing AG 2017. 
    While navigation and interventional guidance are typically based on image data, the images do not necessarily reflect mechanical tissue properties. Optical coherence elastography (OCE) presents a modality with high sensitivity and very high spatial and temporal resolution. However, OCE has a limited field of view of only 2\textendash5 mm depth. We present a side-facing needle probe to image externally induced shear waves from within soft tissue. A first method of quantitative needle-based OCE is provided. Using a time of flight setup, we establish the shear wave velocity and estimate the tissue elasticity. For comparison, an external scan head is used for imaging. Results for four different phantoms indicate a good agreement between the shear wave velocities estimated from the needle probe at different depths and the scan head. The velocities ranging from 0.9\textendash3.4 m/s agree with the expected values, illustrating that tissue elasticity estimates from within needle probes are feasible.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }

2016

  • Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert: Two-photon microscopy using fiber-based nanosecond excitation. Biomedical Optics Express, no. 7, pp. 2432-2440, 2016
    BibTeX Link
    @article{Karpf2016,
       author = {Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert},
       title = {Two-photon microscopy using fiber-based nanosecond excitation},
       journal = {Biomedical Optics Express},
       volume = {7},
       number = {7},
       pages = {2432-2440},
       abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
       keywords = {Fiber optics imaging
    Nonlinear optics, fibers
    Lasers, fiber
    Fluorescence microscopy
    Nonlinear microscopy},
       DOI = {10.1364/BOE.7.002432},
       year = {2016},
       type = {Journal Article}
    }
    
  • Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.: Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. 1-48, 2016
    BibTeX Link
    @article{Schulz-Hildebrandt2016,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.},
       title = {Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {1-48},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584651},
       year = {2016},
       type = {Journal Article}
    }
    
  • Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf: Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results. Investigative Ophthalmology & Visual Science, no. 57, pp. 5852-5852, 2016
    BibTeX Link
    @article{Hüttmann2016,
       author = {Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf},
       title = {Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {57},
       number = {12},
       pages = {5852-5852},
       abstract = {Abstract Purpose : The molecular processes during heating with a photocoagulation laser, particularly in sub-visible or mere thermal stimulation treatment, have only partly been understood, and different theories exist that try to explain its clinical efficacy. Optical coherence tomography (OCT) was successfully used to grade lesions with high accuracy 1 hour after the treatments and beyond. During the irradiation, changes in tissue scattering and, by use of the Doppler signal, tissue motion caused by thermal expansion and coagulation-induced tissue contraction were shown to correlate ex-vivo and in rabbits with the strength of photocoagulation lesions. Aim of this study was to validate feasibility and reproducibility of these results in humans. Methods : In an ongoing study more than 100 lesions of three patients have been imaged with a slitlamp-based OCT (1060 nm, 90,000 A-scans/s) with varying irradiance during laser exposure. Durations of the exposure were 50 ms and 200 ms; spot size was 300 µm. Eye movements and heart beat were corrected by cross-correlation of the images. Increased tissue scattering and movement of the neuronal retina due to thermal expansion were determined from the image sequences with 3 ms temporal resolution. Results : In the first treatments with this prototype device, we received acceptable image quality in 1/3 of the lesions. Changes in the neuronal retina were successful visualized during and after the laser irradiation, demonstrating the feasibility of a real-time assessment of initial effects of photocoagulation in humans. Lesion visibility in standard, reflection-based OCT was much weaker during treatment compared to 1 hour afterwards. Increased tissue scattering was observed in stronger lesions already during the laser irradiation. At reduced irradiance, scattering increase was only observed after the end of irradiation. However, tissue motion towards the vitreous was still observed in these cases. Conclusions : In conclusion, high-speed OCT recording during photocoagulation measures initial tissue changes during photocoagulation in humans. It may enhance our understanding of the tissue dynamics right after laser irradiation. It may provide useful information for a real-time dosage control as well. This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 2: Praktische Aspekte und Anwendung. Augenheilkunde up2date, no. 6, pp. 305-320, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 2: Praktische Aspekte und Anwendung},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {305-320},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-117459},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 1: Physikalische Grundlagen. Augenheilkunde up2date, no. 6, pp. 289-300, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 1: Physikalische Grundlagen},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {289-300},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-113337},
       year = {2016},
       type = {Journal Article}
    }
    
  • Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.: Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. SOP2, 2016
    BibTeX Link
    @article{Pieper2016,
       author = {Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.},
       title = {Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {SOP2},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584654},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy. Journal of Biomedical Optics, no. 21, pp. 078001-078001, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {7},
       pages = {078001-078001},
       note = {10.1117/1.JBO.21.7.078001},
       abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
       ISSN = {1083-3668},
       year = {2016},
       type = {Journal Article}
    }
    
  • Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon: In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography. Optics Letters, no. 41, pp. 4987-4990, 2016
    BibTeX Link
    @article{Sudkamp2016,
       author = {Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon},
       title = {In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography},
       journal = {Optics Letters},
       volume = {41},
       number = {21},
       pages = {4987-4990},
       abstract = {With a simple setup, mainly composed of a low coherence light source and a camera, full-field optical coherence tomography (FF-OCT) allows volumetric tissue imaging. However, fringe washout constrains its use in retinal imaging. Here, we present a novel motion-insensitive approach to FF-OCT, which introduces path-length differences between the reference and the sample light in neighboring pixels using an off-axis reference beam. The temporal carrier frequency in scanned time-domain OCT is replaced by a spatial carrier frequency. Volumetric in-vivo FF-OCT measurements of the human retina were acquired in only 1.3 s, comparable to the acquisition times of current clinically used OCT devices.},
       keywords = {Imaging systems
    Coherence imaging
    Optical coherence tomography
    Frequency filtering
    Interferometric imaging},
    DOI = {10.1364/OL.41.004987}
     url= {https://www.osapublishing.org/ol/abstract.cfm?uri=ol-41-21-4987}  
       year = {2016},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: In vivo optical imaging of physiological responses to photostimulation in human photoreceptors. PNAS Early Edition, pp. 1-6, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {In vivo optical imaging of physiological responses to photostimulation in human photoreceptors},
       journal = {PNAS Early Edition},
       pages = {1-6},
       abstract = {Noninvasive functional imaging of molecular and cellular processes of vision may have immense impact on research and clinical diagnostics. Although suitable intrinsic optical signals (IOSs) have been observed ex vivo and in immobilized animals in vivo, detecting IOSs of photoreceptor activity in living humans was cumbersome and time consuming. Here, we observed clear spatially and temporally resolved changes in the optical path length of the photoreceptor outer segment as a response to an optical stimulus in the living human eye. To witness these changes, we evaluated phase data obtained with a parallelized and computationally aberration-corrected optical coherence tomography system. The noninvasive detection of optical path length changes shows neuronal photoreceptor activity of single cones in living human retina, and therefore, it may provide diagnostic options in ophthalmology and neurology and could provide insights into visual phototransduction in humans.},
       url = {http://www.pnas.org/content/early/2016/10/10/1606428113.abstract},
       year = {2016},
       type = {Journal Article}
    }
    
  • Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.: Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT. Klin Monatsbl Augenheilkd, no. 233, pp. 1324-1330, 2016
    BibTeX Link
    @article{Spahr2016,
       author = {Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.},
       title = {Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT},
       journal = {Klin Monatsbl Augenheilkd},
       volume = {233},
       number = {12},
       pages = {1324-1330},
       ISSN = {0023-2165},
       DOI = {10.1055/s-0042-120279},
       year = {2016},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon: Aberration-free volumetric high-speed imaging of in vivo retina. Scientific Reports, no. 6, pp. 1-11, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon},
       title = {Aberration-free volumetric high-speed imaging of in vivo retina},
       journal = {Scientific Reports},
       volume = {6},
       pages = {1-11},
       url = {http://dx.doi.org/10.1038/srep35209},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.: A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67. Sci Rep, no. 6, pp. 27032, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
       title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
       journal = {Sci Rep},
       volume = {6},
       pages = {27032},
       note = {2045-2322
    Wang, Sijia
    Huttmann, Gereon
    Scholzen, Thomas
    Zhang, Zhenxi
    Vogel, Alfred
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal Article
    England
    Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
       abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
       ISSN = {2045-2322},
       DOI = {10.1038/srep27032},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald: Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits. Investigative Ophthalmology & Visual Science, no. 56, pp. 5980-5980, 2015
    BibTeX
    @article{Hüttmann2015,
       author = {Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald},
       title = {Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {56},
       number = {7},
       pages = {5980-5980},
       ISSN = {1552-5783},
       year = {2015},
       type = {Journal Article}
    }
    
  • Ansari, R. and Buj, C. and Pieper, M. and Konig, P. and Schweikard, A. and Huttmann, G.: Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy. Opt Express, no. 23, pp. 23217-24, 2015
    BibTeX Link
    @article{Ansari2015,
       author = {Ansari, R. and Buj, C. and Pieper, M. and Konig, P. and Schweikard, A. and Huttmann, G.},
       title = {Micro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy},
       journal = {Opt Express},
       volume = {23},
       number = {18},
       pages = {23217-24},
       note = {1094-4087
    Ansari, Rehman
    Buj, Christian
    Pieper, Mario
    Konig, Peter
    Schweikard, Achim
    Huttmann, Gereon
    Journal Article
    United States
    Opt Express. 2015 Sep 7;23(18):23217-24. doi: 10.1364/OE.23.023217.},
       abstract = {Motile cilia perform a range of important mechanosensory and chemosensory functions, along with expulsion of mucus and inhaled pathogens from the lungs. Here we demonstrate that spectral domain optical coherence phase microscopy (SD-OCPM), which combines the principles of optical coherence tomography (OCT) and confocal microscopy, is particularly well-suited for characterization of both morphology and the ciliary dynamics of mouse trachea. We present micro-anatomical images of mouse trachea, where different cell types can be clearly visualized. The phase contrast, which measures the sub-nanometer changes in axial optical pathlength is used to determine the frequency and direction of cilia beatings.},
       ISSN = {1094-4087},
       DOI = {10.1364/oe.23.023217},
       year = {2015},
       type = {Journal Article}
    }
    
  • Diddens-Tschoeke, Heyke C. and Hüttmann, Gereon and Gruber, Achim D. and Pottier, Roy H. and Hanken, Henning: Localized thermal tumor destruction using dye-enhanced photothermal tumor therapy. Lasers in Surgery and Medicine, pp. n/a-n/a, 2015
    BibTeX Link
    @article{Diddens-Tschoeke2015,
       author = {Diddens-Tschoeke, Heyke C. and Hüttmann, Gereon and Gruber, Achim D. and Pottier, Roy H. and Hanken, Henning},
       title = {Localized thermal tumor destruction using dye-enhanced photothermal tumor therapy},
       journal = {Lasers in Surgery and Medicine},
       pages = {n/a-n/a},
       keywords = {photothermal therapy
    naphthalocyanine derivative
    in-vivo
    laser
    dye-enhanced},
       ISSN = {1096-9101},
       DOI = {10.1002/lsm.22356},
       url = {http://dx.doi.org/10.1002/lsm.22356},
       year = {2015},
       type = {Journal Article}
    }
    
    
  • Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.: Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67. Mol Pharm, 2015
    BibTeX Link
    @article{Wang2015,
       author = {Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.},
       title = {Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67},
       journal = {Mol Pharm},
       note = {1543-8392
    Wang, Sijia
    Huttmann, Gereon
    Zhang, Zhenxi
    Vogel, Alfred
    Birngruber, Reginald
    Tangutoori, Shifalika
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal article
    Mol Pharm. 2015 Aug 13.},
       abstract = {The selective inhibition of intracellular and nuclear molecules such as Ki-67 holds great promise for the treatment of cancer and other diseases. However, the choice of the target protein and the intracellular delivery of the functional agent remain crucial challenges. Main hurdles are (a) an effective delivery into cells, (b) endosomal escape of the delivered agents, and (c) an effective, externally triggered destruction of cells. Here we show a light-controlled two-step approach for selective cellular delivery and cell elimination of proliferating cells. Three different cell-penetrating nano constructs, including liposomes, conjugates with the nuclear localization sequence (NLS), and conjugates with the cell penetrating peptide Pep-1, delivered the light activatable antibody conjugate TuBB-9-FITC, which targets the proliferation associated protein Ki-67. HeLa cells were treated with the photosensitizer benzoporphyrin monoacid derivative (BPD) and the antibody constructs. In the first optically controlled step, activation of BPD at 690 nm triggered a controlled endosomal escape of the TuBB-9-FITC constructs. In more than 75% of Ki-67 positive, irradiated cells TuBB-9-FITC antibodies relocated within 24 h from cytoplasmic organelles to the cell nucleus and bound to Ki-67. After a second light irradiation at 490 nm, which activated FITC, cell viability decreased to approximately 13%. Our study shows an effective targeting strategy, which uses light-controlled endosomal escape and the light inactivation of Ki-67 for cell elimination. The fact that liposomal or peptide-assisted delivery give similar results leads to the additional conclusion that an effective mechanism for endosomal escape leaves greater variability for the choice of the delivery agent.},
       keywords = {endosomal entrapment
    liposome
    nanotechnology
    nuclear localization sequence (NLS)
    photodynamic therapy},
       ISSN = {1543-8384},
       DOI = {10.1021/acs.molpharmaceut.5b00260},
       year = {2015},
       type = {Journal Article}
    }
    
  • Klinger, A. and Krapf, L. and Orzekowsky-Schroeder, R. and Koop, N. and Vogel, A. and Huttmann, G.: Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation. J Biomed Opt, no. 20, pp. 116001, 2015
    BibTeX Link
    @article{Klinger2017,
       author = {Klinger, A. and Krapf, L. and Orzekowsky-Schroeder, R. and Koop, N. and Vogel, A. and Huttmann, G.},
       title = {Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation},
       journal = {J Biomed Opt},
       volume = {20},
       number = {11},
       pages = {116001},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.20.11.116001},
       year = {2015},
       type = {Journal Article}
    }
    
  • Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.: Improved endoscopic optical coherence microscopy for imaging of humans airways in patients. Pneumologie, no. 69, pp. A49, 2015
    BibTeX Link
    @article{Schulz-Hildebrandt2015,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.},
       title = {Improved endoscopic optical coherence microscopy for imaging of humans airways in patients},
       journal = {Pneumologie},
       volume = {69},
       number = {07},
       pages = {A49},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0035-1556641},
       year = {2015},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography. Optics Letters, no. 40, pp. 4771-4774, 2015
    BibTeX Link Link
    @article{Spahr2015,
       author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography},
       journal = {Optics Letters},
       volume = {40},
       number = {20},
       pages = {4771-4774},
       abstract = {We demonstrate a new noninvasive method to assess biomechanical properties of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. The motion of retinal tissue that is induced by expansion of the vessels therein is measured with an accuracy of about 10 nm. The pulse shapes of arterial and venous pulsations, their temporal delays, as well as the frequency-dependent pulse propagation through the capillary bed, are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
       keywords = {Optical coherence tomography
    Ophthalmology
    Time-resolved imaging
    Functional monitoring and imaging},
       DOI = {10.1364/OL.40.004771},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-40-20-4771},
       year = {2015},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Hillmann, Dierck and Huttmann, Gereon: Functional Microangiography of in vivo human retina by Full-Field OCT. Investigative Ophthalmology & Visual Science, no. 56, pp. 5974-5974, 2015
    BibTeX Link
    @article{Spahr2015,
       author = {Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Hillmann, Dierck and Huttmann, Gereon},
       title = {Functional Microangiography of in vivo human retina by Full-Field OCT},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {56},
       number = {7},
       pages = {5974-5974},
       abstract = { PurposeOCT based functional microangiography of the retina requires high speed acquisition of a large number of volumetric datasets. Imaging speed of conventional scanning OCT devices is limited by the applicable radiant power and the mechanics used to scan the focused beam over the desired field of view. Full-Field Swept-Source OCT (FF-SS-OCT) resolves both issues, using an areal illumination, which dramatically increases the allowed amount of radiation, and an ultrafast camera for a highly parallelized acquisition.  MethodsThe retina of healthy volunteers was illuminated with wavelengths between 816 and 867 nm by the extended beam of a tunable laser (Broadsweeper, Superlum). Retinal irradiance was below the maximum permissable exposure (MPE). Light backscattered from the retina was imaged onto an ultrafast CMOS camera (SA-Z, Photron), where it interfered with an extended reference beam. From a series of interference images at different wavelengths, volumetric OCT images of the retina were reconstructed.  ResultsWe demonstrate in vivo retinal imaging at 9.9 billion voxels per second (40 million A-scans/s with 256 axial pixels). Sacrificing depth resolution by reducing the number of axial pixels, the A-scan rate was increased to more than 1 billion A-scans per second. FF-SS-OCT allowed imaging of all important retinal structures with good quality at unprecedented imaging speed (see fig. 1). Fast volumetric imaging at up to 3000 volumes/s was used to visualize small capillaries and to analyze the pulsation of retinal arteries and veins (see fig. 2). Imaging time for an area of 4 mm x 2 mm (896 x 368 A-scans) was only 316 µs. The high volume rate and the inherent phase stability enabled quantitative measurement of the change of retinal thickness due to blood pulsation with approx. 10 nm precision. A delay of the venous pulsation with respect to the arteries was observed (approx. 11 ms). The amplitudes of higher frequency components of the venous pulsation were considerably attenuated.  ConclusionsFF-SS-OCT provides fast volumetric imaging of the retina with good image quality. The capillary network can be analyzed with high spatial and temporal resolution. Analysis of retinal pulsation may provide information on pathological changes of vessels and capillaries. Angiographic OCT acquired with the FF-SS-OCT setup. Functional angiography showing the pulsation of retinal artery and vein.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2015},
       type = {Journal Article}
    }
    

2014

  • Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon: Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy. Optics Letters, no. 39, pp. 45-47, 2014
    BibTeX Link
    @article{Ansari2014,
       author = {Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon},
       title = {Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy},
       journal = {Optics Letters},
       volume = {39},
       number = {1},
       pages = {45-47},
       abstract = {We present an ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy (SD-OCPM) system that combines submicrometer transverse spatial resolution and subnanometer optical path length sensitivity, with an acquisition speed of over 217,000  voxels/s. The proposed SD-OCPM system overcomes two significant drawbacks of traditional common-path interferometers—limited transverse spatial resolution and suboptimal detection sensitivity—while maintaining phase stability that is comparable with common-path interferometer setups. The transverse and axial spatial resolution of the setup is measured to be 0.6 and 1.9 μm, respectively, with a phase sensitivity of 0.0027 rad (corresponds to optical path length sensitivity of 110 pm). High-speed acquisition allows for phase-sensitive 4D imaging of biological samples with subcellular resolution.},
       keywords = {Microscopy
    Coherence imaging
    Three-dimensional microscopy},
       DOI = {10.1364/OL.39.000045},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-39-1-45},
       year = {2014},
       type = {Journal Article}
    }
    
  • Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon: Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy. Optics Letters, no. 39, pp. 45-47, 2014
    BibTeX Link Link
    @article{Ansari2014,
       author = {Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon},
       title = {Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy},
       journal = {Optics Letters},
       volume = {39},
       number = {1},
       pages = {45-47},
       abstract = {We present an ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy (SD-OCPM) system that combines submicrometer transverse spatial resolution and subnanometer optical path length sensitivity, with an acquisition speed of over 217,000  voxels/s. The proposed SD-OCPM system overcomes two significant drawbacks of traditional common-path interferometers—limited transverse spatial resolution and suboptimal detection sensitivity—while maintaining phase stability that is comparable with common-path interferometer setups. The transverse and axial spatial resolution of the setup is measured to be 0.6 and 1.9 μm, respectively, with a phase sensitivity of 0.0027 rad (corresponds to optical path length sensitivity of 110 pm). High-speed acquisition allows for phase-sensitive 4D imaging of biological samples with subcellular resolution.},
       keywords = {Microscopy
    Coherence imaging
    Three-dimensional microscopy},
       DOI = {10.1364/OL.39.000045},
       url = {http://ol.osa.org/abstract.cfm?URI=ol-39-1-45},
       year = {2014},
       type = {Journal Article}
    }
    
  • Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino: Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography. European Archives of Oto-Rhino-Laryngology, pp. 1-7, 2014
    BibTeX Link
    @article{Guder2014,
       author = {Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino},
       title = {Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography},
       journal = {European Archives of Oto-Rhino-Laryngology},
       pages = {1-7},
       keywords = {Optical coherence tomography
    Tympanic membrane
    Chronic myringitis},
       ISSN = {0937-4477},
       DOI = {10.1007/s00405-014-3373-z},
       url = {http://dx.doi.org/10.1007/s00405-014-3373-z},
       year = {2014},
       type = {Journal Article}
    }
    

2013

  • Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon: Towards microscopic resolution in holoscopy. pp. 85711O-85711O, 2013
    BibTeX Link Link
    @article{Franke2013,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon},
       title = {Towards microscopic resolution in holoscopy},
       pages = {85711O-85711O},
       note = {10.1117/12.2006806},
       abstract = {Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.},
       DOI = {10.1117/12.2006806},
       url = {http://dx.doi.org/10.1117/12.2006806},
       year = {2013},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon: Off-axis full-field swept-source optical coherence tomography using holographic refocusing. pp. 857104-857104, 2013
    BibTeX Link Link
    @article{Hillmann2013,
       author = {Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon},
       title = {Off-axis full-field swept-source optical coherence tomography using holographic refocusing},
       pages = {857104-857104},
       note = {10.1117/12.2006436},
       abstract = {We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.},
       DOI = {10.1117/12.2006436},
       url = {http://dx.doi.org/10.1117/12.2006436},
       year = {2013},
       type = {Journal Article}
    }
    

2012

  • Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon: Efficient holoscopy image reconstruction. Opt. Express, no. 20, pp. 21247-21263, 2012
    BibTeX
    @article{Hillmann2012,
       author = {Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon},
       title = {Efficient holoscopy image reconstruction},
       journal = {Opt. Express},
       volume = {20},
       number = {19},
       pages = {21247-21263},
       abstract = {Holoscopy is a tomographic imaging technique that combines digital holography and Fourier-domain optical coherence tomography (OCT) to gain tomograms with diffraction limited resolution and uniform sensitivity over several Rayleigh lengths. The lateral image information is calculated from the spatial interference pattern formed by light scattered from the sample and a reference beam. The depth information is obtained from the spectral dependence of the recorded digital holograms. Numerous digital holograms are acquired at different wavelengths and then reconstructed for a common plane in the sample. Afterwards standard Fourier-domain OCT signal processing achieves depth discrimination. Here we describe and demonstrate an optimized data reconstruction algorithm for holoscopy which is related to the inverse scattering reconstruction of wavelength-scanned full-field optical coherence tomography data. Instead of calculating a regularized pseudoinverse of the forward operator, the recorded optical fields are propagated back into the sample volume. In one processing step the high frequency components of the scattering potential are reconstructed on a non-equidistant grid in three-dimensional spatial frequency space. A Fourier transform yields an OCT equivalent image of the object structure. In contrast to the original holoscopy reconstruction with backpropagation and Fourier transform with respect to the wavenumber, the required processing time does neither depend on the confocal parameter nor on the depth of the volume. For an imaging NA of 0.14, the processing time was decreased by a factor of 15, at higher NA the gain in reconstruction speed may reach two orders of magnitude.},
       keywords = {Image processing
    Optical coherence tomography
    Digital holography},
       year = {2012}
    }
    
  • Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.: Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy. Histochem Cell Biol, no. 137, pp. 269-278, 2012
    BibTeX Link
    @article{Klinder2017,
       author = {Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.},
       title = {Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy},
       journal = {Histochem Cell Biol},
       volume = {137},
       number = {3},
       pages = {269-278},
       ISSN = {1432-119X (Electronic)
    0948-6143 (Linking)},
       DOI = {10.1007/s00418-011-0905-0},
       year = {2012},
       type = {Journal Article}
    }
    
  • Hillmann, D. and Bonin, T. and Luhrs, C. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Common approach for compensation of axial motion artifacts in swept-source OCT and dispersion in Fourier-domain OCT. Opt Express, no. 20, pp. 6761-6776, Mar, 2012
    BibTeX
    @article{Hillmann2012,
       author={Hillmann, D.  and Bonin, T.  and Luhrs, C.  and Franke, G.  and Hagen-Eggert, M.  and Koch, P.  and Huttmann, G. },
       title={{{C}ommon approach for compensation of axial motion artifacts in swept-source {O}{C}{T} and dispersion in {F}ourier-domain {O}{C}{T}}},
       journal={Opt Express},
       year={2012},
       volume={20},
       number={6},
       pages={6761--6776},
       month={Mar}
    }
    
  • Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon: Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction. Journal of Biomedical Optics, no. 17, pp. 058003, 2012
    BibTeX
    @article{Rudnitzki2012,
       author = {Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon},
       title = {Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {5},
       pages = {058003},
       year = {2012}
    }

2011

  • Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba: Targeted molecular effects through laser-irradiated nanoabsorbers. 2011
    BibTeX Link
    @inproceedings{Hüttmann2011,
       author = {Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba},
       title = {Targeted molecular effects through laser-irradiated nanoabsorbers},
       editor = {Newsrrom, SPIE},
       DOI = {10.1117/2.1201102.003548},
       type = {Conference Proceedings},
       year = { 2011}
    }
    
  • Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus: Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels. PLoS One, no. 6, pp. e26253, 2011
    BibTeX
    @article{Steven2011,
       author = {Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus},
       title = {Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels},
       journal = {PLoS One},
       volume = {6},
       number = {10},
       pages = {e26253},
       abstract = {<sec> <title>Background</title> <p>The role of lymphatic vessels in tissue and organ transplantation as well as in tumor growth and metastasis has drawn great attention in recent years.</p> </sec> <sec> <title>Methodology/Principal Findings</title> <p>We now developed a novel method using non-invasive two-photon microscopy to simultaneously visualize and track specifically stained lymphatic vessels and autofluorescent adjacent tissues such as collagen fibrils, blood vessels and immune cells in the mouse model of corneal neovascularization in vivo. The mouse cornea serves as an ideal tissue for this technique due to its easy accessibility and its inducible and modifiable state of pathological hem- and lymphvascularization.</p> <p>Neovascularization was induced by suture placement in corneas of Balb/C mice. Two weeks after treatment, lymphatic vessels were stained intravital by intrastromal injection of a fluorescently labeled LYVE-1 antibody and the corneas were evaluated in vivo by two-photon microscopy (TPM). Intravital TPM was performed at 710 nm and 826 nm excitation wavelengths to detect immunofluorescence and tissue autofluorescence using a custom made animal holder. Corneas were then harvested, fixed and analyzed by histology.</p> <p>Time lapse imaging demonstrated the first in vivo evidence of immune cell migration into lymphatic vessels and luminal transport of individual cells. Cells immigrated within 1–5.5 min into the vessel lumen. Mean velocities of intrastromal corneal immune cells were around 9 µm/min and therefore comparable to those of T-cells and macrophages in other mucosal surfaces.</p> </sec> <sec> <title>Conclusions</title> <p>To our knowledge we here demonstrate for the first time the intravital real-time transmigration of immune cells into lymphatic vessels. Overall this study demonstrates the valuable use of intravital autofluorescence two-photon microscopy in the model of suture-induced corneal vascularizations to study interactions of immune and subsequently tumor cells with lymphatic vessels under close as possible physiological conditions.</p> </sec>},
       year = {2011}
    }
  • Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon: In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence. Journal of Biomedical Optics, no. 16, pp. 116025, 2011
    BibTeX
    @article{Orzekowsky2011,
       author = {Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon},
       title = {In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence},
       journal = {Journal of Biomedical Optics},
       volume = {16},
       number = {11},
       pages = {116025},
       keywords = {biological organs
    biomedical optical imaging
    cellular biophysics
    fluorescence
    laser applications in medicine
    two-photon processes},
       year = {2011}
    }
  • Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.: Holoscopy-holographic optical coherence tomography. Opt Lett, no. 36, pp. 2390-2, 2011
    BibTeX Link Link
    @article{Hillmann2011,
       author = {Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.},
       title = {Holoscopy--holographic optical coherence tomography},
       journal = {Opt Lett},
       volume = {36},
       number = {13},
       pages = {2390-2},
       abstract = {Scanning optical coherence tomography (OCT) is limited in sensitivity and resolution by the restricted focal depth of the confocal detection scheme. Holoscopy, a combination of holography and Fourier-domain full-field OCT, is proposed as a way to detect photons from all depths of a sample volume simultaneously with uniform sensitivity and lateral resolution, even at high NAs. By using the scalar diffraction theory, as frequently applied in digital holographic imaging, we fully reconstruct the object field with depth-invariant imaging quality. In vivo imaging of human skin is demonstrated with an image quality comparable to conventionally scanned OCT.},
       keywords = {Fingers
    Fourier Analysis
    Holography/*methods
    Humans
    Photons
    Tomography, Optical Coherence/*methods},
       ISSN = {1539-4794 (Electronic)
    0146-9592 (Linking)},
       DOI = {10.1364/OL.36.002390},
       url = {http://www.ncbi.nlm.nih.gov/pubmed/21725421},
       year = {2011},
       type = {Journal Article}
    }
    
  • Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan: Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell. PLoS Pathog, no. 7, pp. e1002108, 2011
    BibTeX
    @article{Szaszak,
       author = {Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan},
       title = {Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell},
       journal = {PLoS Pathog},
       volume = {7},
       number = {7},
       pages = {e1002108},
       abstract = {<title>Author Summary</title> <p>Separate analysis of host and pathogen metabolic changes in intracellular <italic>C. trachomatis</italic> infections is arduous and has not been comprehensively realized so far. A more detailed understanding about the metabolic activity and needs of <italic>C. trachomatis</italic> and its specific interactions with the host cell would be the basis for the development of novel treatment strategies. We therefore applied fluorescence lifetime imaging (FLIM) of the metabolic coenzymes NAD(P)H using two-photon microscopy to directly visualize metabolic changes of host cells and pathogens in living cells. NAD(P)H fluorescence was detected both on the chlamydial inclusion membrane and inside the inclusion. Interestingly, changes in chlamydial growth and progeny induced by glucose starvation and IFN? treatment were directly linked to significant changes of the NAD(P)H fluorescence lifetimes inside the inclusions. Furthermore, measurement of the NAD(P)H fluorescence lifetime in the host cell nucleus revealed that infected cells were programmed for starvation during the metabolically active phase of intracellular chlamydial growth. Our findings highlight for the first time a direct interaction between host and pathogen metabolism in intracellular bacterial infections that exceeds sole competition for nutrients. In conclusion, fluorescence lifetime imaging of NAD(P)H by two-photon microscopy enables real-time analysis of metabolic host-pathogen interactions in intracellular infections with high spatial and temporal resolution.</p>},
       year = {2011}
    }
  • Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.: Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy. Laser Physics, no. 21, pp. 130-136, 2011
    BibTeX
    @article{Wang,
       author = {Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.},
       title = {Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy},
       journal = {Laser Physics},
       volume = {21},
       number = {1},
       pages = {130-136},
       keywords = {Physik und Astronomie},
       year = {2011}
    }

2010

  • Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon: Optical coherence tomography with online visualization of more than seven rendered volumes per second. Journal of Biomedical Optics, no. 15, pp. 026014, 2010
    BibTeX
    @article{Probst2010,
       author = {Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon},
       title = {Optical coherence tomography with online visualization of more than seven rendered volumes per second},
       journal = {Journal of Biomedical Optics},
       volume = {15},
       number = {2},
       pages = {026014},
       keywords = {image resolution
    medical image processing
    optical tomography
    rendering (computer graphics)
    surgery},
       year = {2010}
    }
  • Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.: Optical coherence tomography for process control of laser micromachining. Rev Sci Instrum, no. 81, pp. 033705, 2010
    BibTeX
    @article{Wiesner2010,
       author = {Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.},
       title = {Optical coherence tomography for process control of laser micromachining},
       journal = {Rev Sci Instrum},
       volume = {81},
       number = {3},
       pages = {033705},
       note = {Using Smart Source Parsing
    Mar},
       abstract = {In situ surface imaging for nondestructive evaluation (NDE) by optical coherence tomography (OCT) before, during, and after ablative laser processing is presented. Furthermore, it is shown that the ability of in situ characterization is beneficial for samples such as optical fibers, which are difficult to handle in the standard analysis. Surface images taken by the OCT are compared with these common analysis tools such as scanning electron microscopy (SEM), reflected-light, and confocal microscopy. An axial resolution of approximately 126 nm for surface detection and a lateral resolution <2.5 microm are obtained and the potential of the setup to imaging structures with high aspect ratio is demonstrated.},
       year = {2010}
    }
  • Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s. Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432., 2010
    BibTeX
    @book{Bonin2010,
       author = {Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s},
       publisher = {Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432.},
       abstract = {In vivo full-field (FF) optical coherence tomography (OCT) images of human retina are presented by using a rapidly tunable laser source in combination with an ultra-high-speed camera. Fourier-domain FF-OCT provided a way to increase the speed of retinal imaging by parallel acquisition of A-scans. Reduced contrast caused by cross talk was observed only below the retinal pigment epithelium. With a 100Hz sweep rate, FF-OCT was fast enough to acquire OCT images with acceptable motion artifacts. FF-OCT allows ultrafast retinal imaging, boosting image speed by a lack of moving parts and a considerably higher irradiation power.},
      year={2010}
    }

2009

  • Hüttmann, G.: Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review. Klinische Monatsblätter für Augenheilkunde, no. 226, pp. 958, 2009
    BibTeX
    @article{Hüttmann2009,
       author = {Hüttmann, G.},
       title = {Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review},
       journal = {Klinische Monatsblätter für Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958},
       year = {2009}
    }
  • Probst, J. and Koch, P. and Hüttmann, G: Real Time 3D Rendering of Coherence Tomography Volumetric Data. 14.-16.06.2009, 2009
    BibTeX
    @techreport{Probst,
       author = {Probst, J. and Koch, P. and Hüttmann, G},
       title = {Real Time 3D Rendering of Coherence Tomography Volumetric Data},
       month = {14.-16.06.2009},
       year = {2009}
    }
  • Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.: Optische Kohärenztomographie in der Mittelohrchirurgie. HNO, no. 57, pp. 421-427, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.},
       title = {Optische Kohärenztomographie in der Mittelohrchirurgie},
       journal = {HNO},
       volume = {57},
       number = {5},
       pages = {421-427},
       abstract = {Die Anwendung der optischen Kohärenztomographie (OCT) in der Mittelohrchirurgie gilt als vielversprechendes Diagnostikum, insbesondere wenn es um die intraoperative Abklärung der Ursache einer Stapesfixation, die intraoperative Beurteilung der Stapesfußplatte bei Revisionsstapesplastik und zur Orientierung bei der Kochleaimplantation, vorwiegend bei Missbildungen, geht. Die OCT bildet Mittel- und Innenohrstrukturen präzise ab und ermöglicht, diese zur Therapiefestlegung zu nutzen. Aus den eigenen bisherigen Ex- und In-vivo-Untersuchungen mit einem OCT-System, das an ein Operationsmikroskop gekoppelt ist, lassen sich derzeit – unter Berücksichtigung der bislang publizierten experimentellen Arbeiten – potenzielle Anwendungsgebiete definieren: Darstellung der ovalen Fensternische bei Revisionsstapes- und rekonstruktiver Mittelohrchirurgie sowie bei der explorativen Tympanotomie zur Abklärung von Perilymphfisteln und Visualisierung von Strukturen des nicht eröffneten Innenohrs.},
       keywords = {Medizin},
       year = {2009}
    }
  • Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.: Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review. Klinische Monatsblatter Fur Augenheilkunde, no. 226, pp. 958-964, 2009
    BibTeX Link
    @article{Hüttmann2009,
       author = {Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.},
       title = {Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review},
       journal = {Klinische Monatsblatter Fur Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958-964},
       note = {542VT
    Times Cited:6
    Cited References Count:48},
       abstract = {Background: Optical coherence tomography (OCT) is new diagnostic procedure that has rapidly evolved in the last years. The recently developed spectral domain OCT allows one to increase the imaging speed by a hundred times compared to the first generation time domain OCT and enables three-dimensional imaging as well as real-time imaging of fast moving structures. Volumetric imaging improves the quantitative measurement of morphology and the evaluation of temporal changes. In addition, an exact correlation with images acquired with other imaging modalities is possible. Real-time imaging enables also the use of OCT during examinations with the slit-lamp and during ophthalmological surgery.
    Methods: A spectral domain OCT was adapted to a slit lamp. 70 patients (91 eyes) were examined at the anterior or posterior segment of the eye. Images of healthy structures and different pathologies were compared to OCT images obtained with Stratus 3, Spectralis, and the SL-OCT. To demonstrate the feasibility of OCT during surgery, spectral domain OCT devices working with 20000 and 210000 A scans per second were coupled by specially developed optics to the camera port of a surgical microscope. The device was tested with phantoms and enucleated pig eyes.
    Results and Conclusions: A5 kHz spectral domain OCT can image the retina during slit lamp-based indirect ophthalmoscopy with a quality similar to that of the Stratus 3. In addition, relevant structures of the anterior segment were imaged. Here, compared to commercially available devices, the imaged field was smaller and the angle of the anterior chamber was not directly visible due to the 830 nm wavelength, which had to be used for retinal imaging. Through the surgical microscope, a volumetric imaging of epithelium, Bowman's, Descemet's membranes, limbus, iris, lens, conjunctiva and sclera was demonstra-ted with several tens of centimetre working distance. Instruments and incisions in the cornea were visualised with 20 mu m precision. Real-time imaging and visualisation of volumetric OCT data were also demonstrated. In principle, all technical problems of an intraoperative use of OCT have been solved and a clinical trial will start in the near future. OCT has the potential to improve the precision of surgical interventions and may even enable new interventions.},
       keywords = {physiological optics
    cornea
    anatomy
    in-vivo
    ultrahigh-resolution
    anterior segment
    time-domain
    blood-flow
    eye
    surgery
    oct
    cyclophotocoagulation
    cornea},
       ISSN = {0023-2165},
       DOI = {DOI 10.1055/s-0028-1109939},
       url = {<Go to ISI>://WOS:000273527800005},
       year = {2009},
       type = {Journal Article}
    }
    
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Optical coherence tomography of the oval window niche.. J Laryngol Oto, no. 1, pp. 1-6, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Optical coherence tomography of the oval window niche.},
       journal = { J Laryngol Oto},
       volume = {1},
       pages = {1-6},
       year = {2009}
    }
  • Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.: Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density. Acta Neurochir, no. 151, pp. 253-262, 2009
    BibTeX
    @article{Kandelhardt,
       author = {Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.},
       title = {Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density},
       journal = {Acta Neurochir },
       volume = {151},
       pages = {253-262},
       year = {2009}
    }
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Intra-operative application of optical coherence tomography with an operating microscope.. J Laryngol Otol, no. 123, pp. 1027-1030, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Intra-operative application of optical coherence tomography with an operating microscope.},
       journal = {J Laryngol Otol},
       volume = {123},
       pages = {1027-1030},
       year = {2009}
    }
  • Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.: Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection. J Biomed Opt, no. 14, pp. 054034, 2009
    BibTeX
    @article{Yao,
       author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
       title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
       journal = {J Biomed Opt},
       volume = {14},
       pages = {054034},
       note = {Journal article},
       year = {2009}
    }
  • Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.: Imaging of human brain tumor tissue by near-infrared laser coherence tomography. Acta Neurochir (Wien), no. 151, pp. 507-17; discussion 517, 2009
    BibTeX
    @article{Böhringer,
       author = {Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.},
       title = {Imaging of human brain tumor tissue by near-infrared laser coherence tomography},
       journal = {Acta Neurochir (Wien)},
       volume = {151},
       number = {5},
       pages = {507-17; discussion 517},
       note = {Bohringer, H J
    Lankenau, E
    Stellmacher, F
    Reusche, E
    Huttmann, G
    Giese, A
    Austria
    Acta Neurochir (Wien). 2009 May;151(5):507-17; discussion 517. Epub 2009 Apr 3.},
       abstract = {INTRODUCTION: Intraoperative detection of residual tumor remains an important challenge in surgery to treat gliomas. New developments in optical techniques offer non-invasive high-resolution imaging that may integrate well into the workflow of neurosurgical operations. Using an intracranial glioma model, we have recently shown that time domain optical coherence tomography (OCT) allows discrimination of normal brain, diffusely invaded brain tissue, and solid tumor. OCT imaging allowed acquisition of 2D and 3D data arrays for multiplanar analysis of the tumor to brain interface. In this study we have analyzed biopsy specimens of human brain tumors and we present the first feasibility study of intraoperative OCT and post-image acquisition processing for non-invasive imaging of the brain and brain tumor. METHODS: We used a Sirius 713 Tomograph with a superluminescence diode emitting light at a near infrared central wavelength of 1,310 nm and a coherence length of 15 microm. The light is passed through an optical mono mode fiber to a modified OCT adapter containing a lens system with a working distance of 10 cm and an integrated pilot laser. Navigation-registered tumor biopsies were imaged ex vivo and the intraoperative site of optical tissue analysis was registered by marker acquisition using a neuronavigation system. RESULTS: Optical coherence tomography non-contact measurements of brain and brain tumor tissue produced B-scan images of 4 mm in width and 1.5-2.0 mm in depth at an axial and lateral optical resolution of 15 microm. OCT imaging demonstrated a different microstructure and characteristic signal attenuation profiles of tumor versus normal brain. Post-image acquisition processing and automated detection of the tissue to air interface was used to realign A-scans to compensate for image distortions caused by pulse- and respiration-induced movements of the target volume. Realigned images allowed monitoring of intensity changes within the scan line and facilitated selection of areas for the averaging of A-scans and the calculation of attenuation coefficients for specific regions of interest. CONCLUSION: This feasibility study has demonstrated that OCT analysis of the tissue microstructure and light attenuation characteristics discriminate normal brain, areas of tumor infiltrated brain, solid tumor, and necrosis. The working distance of the OCT adapter and the A-scan acquisition rate conceptually allows integration of the OCT applicator into the optical path of the operating microscopes. This would allow a continuous analysis of the resection plain, providing optical tomography, thereby adding a third dimension to the microscopic view and information on the light attenuation characteristics of the tissue.},
       keywords = {Biopsy
    Brain Neoplasms/*diagnosis/pathology/surgery
    Feasibility Studies
    Glioma/*diagnosis/pathology/surgery
    Humans
    Monitoring, Intraoperative/methods
    Pilot Projects
    Tomography, Optical Coherence/*methods},
       year = {2009}
    }
  • Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon: Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies. Journal of Biomedical Optics, no. 14, pp. 064040-064040, 2009
    BibTeX
    @article{Steven,
       author = {Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon},
       title = {Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies},
       journal = {Journal of Biomedical Optics},
       volume = {14},
       number = {6},
       pages = {064040-064040},
       note = {10.1117/1.3275475},
       abstract = {Minimally invasive imaging of ocular surface pathologies aims at securing clinical diagnosis without actual tissue probing. For this matter, confocal microscopy (Cornea Module) is in daily use in ophthalmic practice. Multiphoton microscopy is a new optical technique that enables high-resolution imaging and functional analysis of living tissues based on tissue autofluorescence. This study was set up to compare the potential of a multiphoton microscope (DermaInspect) to the Cornea Module. Ocular surface pathologies such as pterygia, papillomae, and nevi were investigated in vivo using the Cornea Module and imaged immediately after excision by DermaInspect. Two excitation wavelengths, fluorescence lifetime imaging and second-harmonic generation (SHG), were used to discriminate different tissue structures. Images were compared with the histopathological assessment of the samples. At wavelengths of 730nm, multiphoton microscopy exclusively revealed cellular structures. Collagen fibrils were specifically demonstrated by second-harmonic generation. Measurements of fluorescent lifetimes enabled the highly specific detection of goblet cells, erythrocytes, and nevus-cell clusters. At the settings used, DermaInspect reaches higher resolutions than the Cornea Module and obtains additional structural information. The parallel detection of multiphoton excited autofluorescence and confocal imaging could expand the possibilities of minimally invasive investigation of the ocular surface toward functional analysis at higher resolutions.},
       year = {2009}
    }

2008

  • Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W: Optically controlled thermal management on the nanometer length scale. Nanotechnology, no. 19, pp. 055207, 2008
    BibTeX
    @article{Garwe,
       author = {Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W},
       title = {Optically controlled thermal management on the nanometer length scale},
       journal = {Nanotechnology},
       volume = {19},
       number = {5},
       pages = {055207},
       abstract = {The manipulation of polymers and biological molecules or the control of chemical reactions on a nanometer scale by means of laser pulses shows great promise for applications in modern nanotechnology, biotechnology, molecular medicine or chemistry. A controllable, parallel, highly efficient and very local heat conversion of the incident laser light into metal nanoparticles without ablation or fragmentation provides the means for a tool like a 'nanoreactor', a 'nanowelder', a 'nanocrystallizer' or a 'nanodesorber'. In this paper we explain theoretically and show experimentally the interaction of laser radiation with gold nanoparticles on a polymethylmethacrylate (PMMA) layer (one-photon excitation) by means of different laser pulse lengths, wavelengths and pulse repetition rates. To the best of our knowledge this is the first report showing the possibility of highly local (in a 40 nm range) regulated heat insertion into the nanoparticle and its surroundings without ablation of the gold nanoparticles. In an earlier paper we showed that near-infrared femtosecond irradiation can cut labeled DNA sequences in metaphase chromosomes below the diffraction-limited spot size. Now, we use gold as well as silver-enhanced gold nanoparticles on DNA (also within chromosomes) as energy coupling objects for femtosecond laser irradiation with single-and two-photon excitation. We show the results of highly localized destruction effects on DNA that occur only nearby the nanoparticles.},
       year = {2008}
    }
  • Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.: Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental. Laser Surg Med, no. 40, pp. 273-81, 2008
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.},
       title = {Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental},
       journal = {Laser Surg Med},
       volume = {40
    },
       number = {4},
       pages = {273-81},
       year = {2008}
    }
  • Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.: Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes. no. 13, pp. 031217, 2008
    BibTeX
    @misc{Qu,
       author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.},
       title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes},
       volume = {13},
       number = {3},
       pages = {031217},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.},
       ISBN = {1083-3668 (Print)
    1083-3668 (Linking)},
       year = {2008}
    }
  • Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.: Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue. Invest Ophthalmol Vis Sci, no. 49, pp. 1512-7, 2008
    BibTeX
    @article{Steven,
       author = {Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.},
       title = {Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {49},
       number = {4},
       pages = {1512-7},
       note = {Using Smart Source Parsing
    Apr},
       abstract = {PURPOSE: Conjunctiva-associated lymphoid tissue (CALT) is assumed to be a key location for the generation of adaptive immune mechanisms of the ocular surface, but functional studies of CALT are still lacking. The purpose of this study was to establish an animal model that enables functional analysis of immune mechanisms going on within CALT. In addition, the use of two-photon microscopy, a new optical method, was evaluated for examining complex immunologic interactions of CALT by volume (three-dimensional [3-D]) and time-dependence (four-dimensional [4-D]) in vivo. METHODS: The conjunctiva of female BALB/c mice was repeatedly challenged with topical Chlamydia trachomatis serovar C or a solution of ovalbumin and cholera toxin B. Two-photon microscopy was conducted on explanted, unfixed, and unstained eyes with adjacent nictitating membranes. RESULTS: After three to five stimulations, CALT was detected exclusively in the nictitating membrane of 73% (C. trachomatis) or 70% (ovalbumin/ cholera toxin) of the animals. CALT mainly consisted of CD45R/B220+ B cells and CD4+ and CD8+ T cells. Electron microscopy showed intraepithelial lymphocytes and follicles consisting of lymphocytes, dendritic cells, and macrophages. Two-photon microscopy based on tissue autofluorescence allowed all components of CALT to be detected three dimensionally. High-resolution images were generated in tissue depths of 65 microm below the mucosal surface. CONCLUSIONS: This study introduces a novel mouse model for functional investigations of CALT. Topical stimulation with C. trachomatis or ovalbumin/cholera toxin B reliably leads to CALT generation at the nictitating membrane. The use of two-photon microscopy enables groundbreaking 3-D and, in the future, intravital 4-D investigations of immunologic processes initiated in CALT.},
       year = {2008}
    }

2007

  • Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.: Principles of laser microdissection and catapulting of histologic specimens and live cells. in Laser Manipulation of Cells and Tissues, Methods in Cell Biology, no. 82, pp. 153-205, Academic Press Elsevier, San Diego, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.},
       title = {Principles of laser microdissection and catapulting of histologic specimens and live cells},
       booktitle = {Laser Manipulation of Cells and Tissues, Methods in Cell Biology},
       editor = {Berns, M.  and Greulich, K.O.},
       publisher = {Academic Press Elsevier},
       address = {San Diego},
       volume = {82},
       pages = {153-205},
       year = {2007}
    }
  • Hüttmann, G. and Löning, M.: Physikalische Grundlagen optischer Technologien. Der Gynäkologe, no. 40, pp. 332-337, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G. and Löning, M.},
       title = {Physikalische Grundlagen optischer Technologien},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {332-337},
       year = {2007}
    }
  • Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.: Optische Kohärenztomographie in der Gynokologie. Der Gynäkologe, no. 40, 2007
    BibTeX
    @article{Löning,
       author = {Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.},
       title = {Optische Kohärenztomographie in der Gynokologie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       year = {2007}
    }
  • Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.: Mechanisms of laser-induced dissection and transport of histologic specimens.. Biophys J, no. 93, pp. 4481-4500, 2007
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.},
       title = {Mechanisms of laser-induced dissection and transport of histologic specimens.},
       journal = {Biophys J},
       volume = {93},
       pages = {4481-4500},
       year = {2007}
    }
  • Vogel, Alfred and Lorenz, Kathrin and Horneffer, Verena and Hüttmann, Gereon and Von Smolinski, Dorthe and Gebert, Andreas: Mechanisms of laser-induced dissection and transport of histologic specimens. Biophysical journal, no. 93, pp. 4481-4500, 2007
    BibTeX
    @article{Vogel2007,
       author = {Vogel, Alfred and Lorenz, Kathrin and Horneffer, Verena and Hüttmann, Gereon and Von Smolinski, Dorthe and Gebert, Andreas},
       title = {Mechanisms of laser-induced dissection and transport of histologic specimens},
       journal = {Biophysical journal},
       volume = {93},
       number = {12},
       pages = {4481-4500},
       ISSN = {0006-3495},
       year = {2007},
       type = {Journal Article}
    }
    
  • Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.: Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues. in The Eigth International Conference on Laser Ablation (COLA 2005), no. 59, pp. 249-254, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues},
       booktitle = {The Eigth International Conference on Laser Ablation (COLA 2005)},
       editor = {Herman, P. and Hess, W .},
       series = {Journal of Physics: Conference Series},
       volume = {59},
       pages = {249-254},
       year = {2007}
    }
  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.: Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography. Curr Eye Res, no. 32, pp. 171-176, 2007
    BibTeX
    @article{Müller,
       author = {Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.},
       title = {Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography},
       journal = {Curr Eye Res},
       volume = {32},
       pages = {171-176},
       year = {2007}
    }
  • Hüttmann, G.: Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie. Der Gynäkologe, no. 40, pp. 372, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G.},
       title = {Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {372},
       year = {2007}
    }
  • Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.: Combining Optical Coherence Tomographie (OCT) with an operating microscope. in Medical Engineering, pp. pp 343-348, Springer, Berlin, Heidelberg, New York, 2007
    BibTeX
    @inbook{Lankenau,
       author = {Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.},
       title = {Combining Optical Coherence Tomographie (OCT) with an operating microscope},
       booktitle = {Medical Engineering},
       editor = {Buzug, T.M. and Holz, D. and Weber, S.  and Bongartz, J. and Kohl-Bareise, M. and Hartmann, U.},
       publisher = {Springer, Berlin, Heidelberg, New York},
       pages = {pp 343-348},
       year = {2007}
    }
  • Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.: Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif, no. 40, pp. 422-30, 2007
    BibTeX
    @article{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.},
       title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis},
       journal = {Cell Prolif},
       volume = {40},
       number = {3},
       pages = {422-30},
       note = {Rahmanzadeh, R
    Huttmann, G
    Gerdes, J
    Scholzen, T
    England
    Cell Prolif. 2007 Jun;40(3):422-30.},
       abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.},
       keywords = {Antibodies, Antinuclear
    Antibodies, Monoclonal
    Cell Division/physiology
    Cell Nucleolus/physiology
    Fluorescein-5-isothiocyanate
    Fluorescent Dyes
    HeLa Cells
    Humans
    Ki-67 Antigen/*genetics/*metabolism
    Photochemistry
    RNA Polymerase I/metabolism
    RNA, Ribosomal/*biosynthesis},
       year = {2007}
    }
  • Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.: Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues. in Handai Nanophotonics, no. Volume 3, pp. 273-286, Elsevier, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues},
       booktitle = {Handai Nanophotonics},
       editor = {Hiroshi Masuhara, Satoshi Kawata and Fumio, Tokunaga},
       publisher = {Elsevier},
       volume = {Volume 3},
       pages = {273-286},
       year = {2007}
    }

2006

  • Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.: Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.. Proc SPIE, no. 6078, pp. 495-502, 2006
    BibTeX
    @article{Giese2006,
       author = {Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.},
       title = {Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.},
       journal = {Proc SPIE},
       volume = {6078},
       pages = {495-502},
       year = {2006},
       type = {Journal Article}
    }
    
  • Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.: Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.. Proc SPIE, no. 6078, pp. 495-502, 2006
    BibTeX
    @article{Giese,
       author = {Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.},
       title = {Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.},
       journal = {Proc SPIE},
       volume = {6078},
       pages = {495-502},
       year = {2006}
    }
  • Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.: Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.. Proc SPIE, no. 6078, pp. 495-502, 2006
    BibTeX
    @article{Giese,
       author = {Giese, A. and Böhringer, H.J. and Leppert, J. and Kandelhardt, S.R. and Lankenau, E. and Koch, P. and Birngruber, R. and Hüttmann, G.},
       title = {Non-invasive intraoperative optical coherence tomography of the resection cavity during surgery of intrinsic brain tumors.},
       journal = {Proc SPIE},
       volume = {6078},
       pages = {495-502},
       year = {2006}
    }
  • Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Hüttmann, G. and Birngruber, R. and Laqua, H.: Filtring Bleb Evaluation with Slit-Lamp-Adapted 1310-nm Optical Coherence Tomography. Curr Eye Res, no. 31, pp. 909-915, 2006
    BibTeX
    @article{Müller2006,
       author = {Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Hüttmann, G. and Birngruber, R. and Laqua, H.},
       title = {Filtring Bleb Evaluation with Slit-Lamp-Adapted 1310-nm Optical Coherence Tomography},
       journal = {Curr Eye Res},
       volume = {31},
       number = {11},
       pages = {909-915},
       year = {2006},
       type = {Journal Article}
    }
    
  • Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Huttmann, G. and Birngruber, R. and Laqua, H.: Filtering bleb evaluation with slit-lamp-adapted 1310-nm optical coherence tomography. Current Eye Research, no. 31, pp. 909-915, 2006
    BibTeX Link
    @article{Müller2006,
       author = {Muller, M. and Hoerauf, H. and Geerling, G. and Pape, S. and Winter, C. and Huttmann, G. and Birngruber, R. and Laqua, H.},
       title = {Filtering bleb evaluation with slit-lamp-adapted 1310-nm optical coherence tomography},
       journal = {Current Eye Research},
       volume = {31},
       number = {11},
       pages = {909-915},
       note = {107AB
    Times Cited:24
    Cited References Count:27},
       abstract = {Purpose: Investigation of slit-lamp-adapted 1310-nm optical coherence tomography (OCT) as an in vivo imaging device in the postoperative course of glaucoma surgery. Methods: Postoperative images of filtering blebs and deep sclerectomies and their healing processes were qualitatively evaluated with a slit-lamp-adapted anterior segment OCT (AS-OCT; Heidelberg Engineering, Heidelberg, Germany) in 28 patients. Ophthalmologic examinations included slit-lamp examination, applanation tonometry, and slit-lamp photography. The OCT scans were qualitatively correlated with the morphologic and functional outcome of the filtering bleb. Results: 1310-nm OCT was able to demonstrate the internal structure and the dimensions of filtering blebs, as well as the scleral flap and the deep sclerectomy location including Descemet membrane. Functioning filtering blebs showed a low OCT signal, small fluid-filled cysts, superficial microcystic layer, and a slack internal texture. High internal reflectivity indicated an earlier scarring of the filtering bleb. Nonfunctioning filtering blebs delivered a high OCT signal, no or few cysts, and a dense internal texture. These different OCT patterns correlated with the clinical outcome. Conclusions: Slit-lamp-adapted 1310-nm OCT allowed the noncontact observation and documentation of the postoperative healing course of filtering blebs after glaucoma surgery. Internal structures of the filtering bleb and deep sclerectomies could be visualized. Functioning and dysfunctioning filtering blebs delivered different OCT pattern and correlated with the clinical outcome. This could be a new way to assess the postoperative healing process with the possibility of earlier intervention in cases of impending scarring.},
       keywords = {deep sclerectomy
    filtering bleb
    oct
    scarring
    anterior segment
    mitomycin-c
    follow-up
    trabeculectomy
    surgery
    cornea
    eye},
       ISSN = {0271-3683},
       DOI = {Doi 10.1080/02713680600910528},
       url = {<Go to ISI>://WOS:000242142000003},
       year = {2006},
       type = {Journal Article}
    }
    
  • Vogel, A. and Noack, J and Hüttmann, G. and Paltauf, G.: Femtosecond plasma-mediated nanosurgery of cells and tissues.. in Laser Ablation, pp. 217-262, Springer, Heidelberg, 2006
    BibTeX
    @inbook{Vogel2006,
       author = {Vogel, A. and Noack, J and Hüttmann, G. and Paltauf, G.},
       title = {Femtosecond plasma-mediated nanosurgery of cells and tissues.},
       booktitle = {Laser Ablation},
       editor = {), Phipps C (Hrsg.},
       publisher = { Springer, Heidelberg},
       pages = {217-262},
       year = {2006}
    }

2005

  • Schüle, G and Rumohr, M and Hüttmann, G and Brinkmann, R: RPE damage thresholds and mechanisms for laser exposure in the µs to ms time regimen. Inv Ophthalmol & Vis Sci, no. 46, pp. 714-719, 2005
    BibTeX
    @article{Schüle,
       author = {Schüle, G and Rumohr, M and Hüttmann, G and Brinkmann, R},
       title = {RPE damage thresholds and mechanisms for laser exposure in the µs to ms time regimen},
       journal = {Inv Ophthalmol & Vis Sci},
       volume = {46},
       number = {2},
       pages = {714-719},
       year = {2005}
    }
  • Hüttmann, Gereon and Yao, Cuiping and Endl, Elmar: New concepts in laser medicine: Towards a laser surgery with cellular precision. Medical laser application, no. 20, pp. 135-139, 2005
    BibTeX
    @article{Hüttmann2005,
       author = {Hüttmann, Gereon and Yao, Cuiping and Endl, Elmar},
       title = {New concepts in laser medicine: Towards a laser surgery with cellular precision},
       journal = {Medical laser application},
       volume = {20},
       number = {2},
       pages = {135-139},
       abstract = {New concepts and instrumentation in laser medicine are driven by the progress in optical technology as well as by advances in the understanding of the interaction of optical irradiation with tissue, especially at a macromolecular scale, and by the changing needs in health care. Complexity and costs of laser sources will decrease due to the use of semiconductor and fiber lasers, and complex or non-linear mechanisms by which the radiation effects tissues are better understood, especially when ultra-short laser pulses are used. Especially femtosecond lasers and nanotechnology have the potential to treat diseases at a cellular level. Focused femtosecond irradiation was successfully used to manipulate tissues with subcellular precision. Laser-irradiated nanoparticles can selectively destroy individual cells.},
       keywords = {Mikro- und Nanowirkungen
    Selective Foto thermolyse
    Faserlaser
    Femtosekundenlaser
    Nanopartikel
    Micro- and nanoeffects
    Selective photothermolysis
    Fiber laser
    Femtosecond laser
    Nanoparticles},
       year = {2005}
    }
  • Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.: Mechanisms of femtosecond laser nanosurgery of biological cells and tissues. Appl. Phys B, no. 81, pp. 1015-1047, 2005
    BibTeX
    @article{Vogel2005,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Mechanisms of femtosecond laser nanosurgery of biological cells and tissues},
       journal = {Appl. Phys B},
       volume = {81},
       pages = {1015-1047},
       year = {2005}
    }
  • Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.: Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles. J Biomed Opt, no. 10, pp. 064012, 2005
    BibTeX
    @article{Yao,
       author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.},
       title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles},
       journal = {J Biomed Opt},
       volume = {10},
       number = {6},
       pages = {064012},
       note = {Yao, Cuiping
    Rahmanzadeh, Ramtin
    Endl, Elmar
    Zhang, Zhenxi
    Gerdes, Johannes
    Huttmann, Gereon
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2005 Nov-Dec;10(6):064012.},
       abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.},
       keywords = {Biopolymers/pharmacokinetics
    Cell Line, Tumor
    Cell Membrane Permeability/ physiology/ radiation effects
    Drug Delivery Systems/ methods
    Fluoresceins/ pharmacokinetics
    Humans
    Lasers
    Lymphoma/ metabolism
    Nanostructures},
       year = {2005}
    }

2004

  • Löning, M. and Diddens, H. and W, Küpker and Diedrich, K. and Hüttmann, G.: Laparoscopic Fluorescence Detection of Ovarian Carcinoma Metastases Using 5-Aminolevulinic Acid-Induced Protoporphyrin IX. no. 100, 2004
    BibTeX
    @article{Löning,
       author = {Löning, M. and Diddens, H. and W, Küpker and Diedrich, K. and Hüttmann, G.},
       title = {Laparoscopic Fluorescence Detection of Ovarian Carcinoma Metastases Using 5-Aminolevulinic Acid-Induced Protoporphyrin IX},
       journal = {CANCER},
       volume = {100},
       number = {8},
       year = {2004}
    }
    
    
  • Daniltchenko, D. and Lankenau, E. and König, F. and Shay, B. and Hüttmann, G. and Sachs, M.D. and Schnorr, D.: In Vivo Optical Coherence Tomography in Endoscopic Diagnosis of Bladder Disease. in Clinical technoligies and systems, no. 5316, pp. 473-478, Proc Soc Photo-Opt Instrum Eng, SPIE 2004, 2004
    BibTeX
    @inbook{Daniltchenko,
       author = {Daniltchenko, D. and Lankenau, E. and König, F. and Shay, B. and Hüttmann, G. and Sachs, M.D. and Schnorr, D.},
       title = {In Vivo Optical Coherence Tomography in Endoscopic Diagnosis of Bladder Disease},
       booktitle = {Clinical technoligies and systems},
       editor = {Vo-Dinh, Tuan},
       publisher = {Proc Soc Photo-Opt Instrum Eng, SPIE 2004},
       volume = {5316},
       pages = {473-478},
       year = {2004}
    }

2003

  • Löning, M. and Lankenau, E. and Strunck, C. and Krokowski, M. and Hillbricht, S. and Diedrich, K. and Hüttmann, G.: Optische Kohärenztomographie - ein neues hochauflösendes Schnittbildverfahren als Ergänzung zur Kolposkopie. Geburtsh Frauenheilk, no. 63, pp. 1158-1161, 2003
    BibTeX
    @article{Löning,
       author = {Löning, M. and Lankenau, E. and Strunck, C. and Krokowski, M. and Hillbricht, S. and Diedrich, K. and Hüttmann, G.},
       title = {Optische Kohärenztomographie - ein neues hochauflösendes Schnittbildverfahren als Ergänzung zur Kolposkopie},
       journal = {Geburtsh Frauenheilk},
       volume = {63},
       pages = {1158-1161},
       year = {2003}
    }
    

2002

  • Hüttmann, G and Houserek, S. and Diddens, H. and Hendrich, C.: Photodynamische Therapie in der Orthopädie. pp. 344-352, 2002
    BibTeX
    @misc{Hüttmann,
       author = {Hüttmann, G and Houserek, S. and Diddens, H. and Hendrich, C.},
       title = {Photodynamische Therapie in der Orthopädie},
       pages = {344-352},
       year = {2002}
    }
    
  • Vogel, A. and Hüttmann, G. and Paltauf, G.: Low-density plasmas below the optical breakdown threshold: potential hazard for multiphoton microscopy, and a tool for the manipulation of intracellular events. Proc SPIE Multiphoton Microscopy in the Biomedical Sciences II, no. 4620, pp. 202-216, 2002
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Hüttmann, G. and Paltauf, G.},
       title = {Low-density plasmas below the optical breakdown threshold: potential hazard for multiphoton microscopy, and a tool for the manipulation of intracellular events},
       journal = {Proc SPIE "Multiphoton Microscopy in the Biomedical Sciences II"},
       volume = {4620},
       pages = {202-216},
       year = {2002}
    }
    
  • Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.: High Precision Cell Surgery with Nanoparticles?. Med Laser Appl, no. 17, pp. 9-14, 2002
    BibTeX
    @article{Hüttmann2002,
       author = {Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.},
       title = {High Precision Cell Surgery with Nanoparticles?},
       journal = {Med Laser Appl},
       volume = {17},
       pages = {9-14},
       year = {2002},
       type = {Journal Article}
    }
    
  • Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.: High Precision Cell Surgery with Nanoparticles?. Med Laser Appl, no. 17, pp. 9-14, 2002
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Radt, B. and Serbin, J. and Lange, B.I. and Birngruber, R.},
       title = {High Precision Cell Surgery with Nanoparticles?},
       journal = {Med Laser Appl},
       volume = {17},
       pages = {9-14},
       year = {2002}
    }
    
  • Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.: Femtosecond-laser-produced low-density plasmas in transparent biological media: A tool for the creation of chemical, thermal and thermomechanical effets below the optical brekdown threshold. Proc. SPIE Commercial and Biological Applications of Ultrafast Lasers IV, no. 4633, pp. 23-37, 2002
    BibTeX
    @article{Vogel, 
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Femtosecond-laser-produced low-density plasmas in transparent biological media: A tool for the creation of chemical, thermal and thermomechanical effets below the optical brekdown threshold},
       journal = {Proc. SPIE "Commercial and Biological Applications of Ultrafast Lasers IV"},
       volume = {4633},
       pages = {23-37},
       year = {2002}
    }
    

2001

  • Hüttmann, G. and Serbin, J. and Radt, B. and Lange, B.I. and Birngruber, R.: Model system for investigating laser-induced subcellular microeffects.. Proc SPIE, no. 4257, pp. 398-409, 2001
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Serbin, J. and Radt, B. and Lange, B.I. and Birngruber, R.},
       title = {Model system for investigating laser-induced subcellular microeffects.},
       journal = {Proc SPIE},
       volume = {4257},
       pages = {398-409},
       year = {2001}
    }
    

2000

  • Schüle, G. and Hüttmann, G. and Roider, J. and Wirbelauer, C. and Birngruber, R. and Brinkmann, R.: Optoacoustic measurements during µs-irradiation of the retinal pigment epithelium. Proc. SPIE, no. 3914A, 2000
    BibTeX
    @article{Schüle,
       author = {Schüle, G. and Hüttmann, G. and Roider, J. and Wirbelauer, C. and Birngruber, R. and Brinkmann, R.},
       title = {Optoacoustic measurements during µs-irradiation of the retinal pigment epithelium},
       journal = {Proc. SPIE},
       volume = {3914A},
       year = {2000}
    }
    
  • Hüttmann, G. and Birngruber, R.: Laserinduzierte thermische Gewebseffekte mit mikroskopischer und makromolekularer Präzision. Z Med Phys, no. 10, pp. 169-174, 2000
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Birngruber, R.},
       title = {Laserinduzierte thermische Gewebseffekte mit mikroskopischer und makromolekularer Präzision},
       journal = {Z Med Phys},
       volume = {10},
       pages = {169-174},
       year = {2000}
    }
    
  • Hüttmann, G. and Radt, B. and Birngruber, R.: Laserinduzierte Mikro- und Nanoeffekte - Von der selektiven Thermolyse zu molekularen Nanoeffekten. LaserOpto, no. 32, pp. 47-55, 2000
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Radt, B. and Birngruber, R.},
       title = {Laserinduzierte Mikro- und Nanoeffekte - Von der selektiven Thermolyse zu molekularen Nanoeffekten},
       journal = {LaserOpto},
       volume = {32},
       pages = {47-55},
       year = {2000}
    }
    

1999

  • Brinkmann, R. and Rögener, J. and Lin, C.P. and Roider, J. and Birngruber, R. and Hüttmann, G.: Selective RPE-Photodestruction: Mechanism of Cell Damage by pulsed laser irradiance in the ns to µs time regime. Proc. SPIE, no. 3601, pp. 59-65, 1999
    BibTeX
    @article{Brinkmann1999,
       author = {Brinkmann, R. and Rögener, J. and Lin, C.P. and Roider, J. and Birngruber, R. and Hüttmann, G.},
       title = {Selective RPE-Photodestruction: Mechanism of Cell Damage by pulsed laser irradiance in the ns to µs time regime},
       journal = {Proc. SPIE},
       volume = {3601},
       pages = {59-65},
       year = { 1999}
    }
    
  • Hüttmann, G. and Birngruber, R.: On the Possibility of High-Precision Photothermal Microeffects and the Measurement of Fast thermal Denaturation of Proteins.. IEEE J Quant Electr, no. 5, pp. 954-962, 1999
    BibTeX
    @article{Hüttmann,
       author = {Hüttmann, G. and Birngruber, R.},
       title = {On the Possibility of High-Precision Photothermal Microeffects and the Measurement of Fast thermal Denaturation of Proteins.},
       journal = {IEEE J Quant Electr},
       volume = {5},
       number = {4},
       pages = {954-962},
       year = {1999}
    }
    
  • Huttmann, G. and Birngruber, R.: On the possibility of high-precision photothermal microeffects and the measurement of fast thermal denaturation of proteins. Ieee Journal of Selected Topics in Quantum Electronics, no. 5, pp. 954-962, 1999
    BibTeX Link
    @article{Hüttmann1999,
       author = {Huttmann, G. and Birngruber, R.},
       title = {On the possibility of high-precision photothermal microeffects and the measurement of fast thermal denaturation of proteins},
       journal = {Ieee Journal of Selected Topics in Quantum Electronics},
       volume = {5},
       number = {4},
       pages = {954-962},
       note = {248CM
    Times Cited:77
    Cited References Count:43},
       abstract = {The precision of laser-induced effects is often limited by thermal and thermomechanical collateral damage. Adjusting the pulsewidth of the laser to the size of the absorbing structure can at least avoid thermal side effects and facilitates a selective treatment of vessels or pigmented cells. Further extending the precision of thermal effects below cellular dimensions by using nanometer sized particles could open up new fields of applications for lasers in medicine and biology. Calculations show that under irradiation with nano- or picosecond laser pulses gold particles of submicrometer size can easily be heated by several hundred K, High temperatures have to be used for subcellular thermal effects, because heat confinement to such small structures requires the thermal damage to occur in extremely short times. Estimating the denaturation temperature by extrapolating the Arrhenius equation from a time range of minutes and seconds into a time range of nano- and picoseconds leads to temperatures beta;een 370 K-470 K, There is evidence that in aqueous media, due to the surface tension, these temperatures can be generated at the surface of nanometer sized particles without vaporization of the surrounding water,
    In order to show whether or not an extrapolation of the damage rates over six to nine orders of magnitude gives correct data, a temperature-jump experiment was designed and tested which allows to measure denaturation rates of proteins in the millisecond time range. Denaturation of chymotrypsin was observed within 300 mu s at temperatures below 380 K, The rate constants for the unfolding of chymotrypsin followed the Arrhenius equation up to rates of 3000 s(-1).},
       keywords = {laser medicine
    microeffects
    protein denaturation
    thermal effects
    assisted laser inactivation},
       ISSN = {1077-260X},
       DOI = {Doi 10.1109/2944.796317},
       url = {<Go to ISI>://WOS:000083257800013},
       year = {1999},
       type = {Journal Article}
    }
    

1998

  • Hüttmann, G. and Birngruber, R.: Dynamics of Thermal Microeffects: Rate Constants of Thermal Denaturation Measured by a Temperature-Jump Experiment. OSA TOPS, no. 22, pp. 300-305, 1998
    BibTeX
    @article{Hüttmann1998,
       author = {Hüttmann, G. and Birngruber, R.},
       title = {Dynamics of Thermal Microeffects: Rate Constants of Thermal Denaturation Measured by a Temperature-Jump Experiment},
       journal = {OSA TOPS},
       volume = {22},
       pages = {300-305},
       year = {1998},
       type = {Journal Article}
    }
    

1997

  • Hendrich, C. and Hüttmann, G. and Lehnert, C. and Diddens, H. and Siebert, W.E.: Photodynamic laser therapy for rheumatoid arthritis: Cell culture studies and animal experiments.. Knee Surg Sports Traumatol Arthroscopy, no. 5, pp. 58-63, 1997
    BibTeX
    @article{Hendrich,
       author = {Hendrich, C. and Hüttmann, G. and Lehnert, C. and Diddens, H. and Siebert, W.E.},
       title = {Photodynamic laser therapy for rheumatoid arthritis: Cell culture studies and animal experiments.},
       journal = {Knee Surg Sports Traumatol Arthroscopy},
       volume = {5},
       pages = {58-63},
       year = {1997}
    }
    

1996

  • Novo, M. and Hüttmann, G. and Diddens, H.: Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumours. J Photoch Photobio, no. 34, pp. 143-148, 1996
    BibTeX
    @article{Novo,
       author = {Novo, M. and Hüttmann, G. and Diddens, H.},
       title = {Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumours},
       journal = {J Photoch Photobio},
       volume = {34},
       pages = {143-148},
       year = {1996}
    }
    

  • Hillmann, Dierck and Huttmann, Gereon and Koch, Peter: Using nonequispaced fast Fourier transformation to process optical coherence tomography signals. no. 7372, pp. 73720R, SPIE,
    BibTeX
    @inproceedings{Hillmann,
       author = {Hillmann, Dierck and Huttmann, Gereon and Koch, Peter},
       title = {Using nonequispaced fast Fourier transformation to process optical coherence tomography signals},
       editor = {Peter, E. Andersen and Brett, E. Bouma},
       publisher = {SPIE},
       volume = {7372},
       pages = {73720R},
    
    }
  • Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon: Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina. no. 7889, pp. 788906, SPIE,
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon},
       title = {Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {788906},
    
    }
  • Hüttmann, G. and Churio, S. and Braslavsky, S.E. and Birngruber, R.: The Possibility of Measuring Thermal Protein Denaturation by an Optoacoustic Method.. in Proc. SPIE, no. 2077, pp. 202-212,
    BibTeX
    @inproceedings{Hüttmann,
       author = {Hüttmann, G. and Churio, S. and Braslavsky, S.E. and Birngruber, R.},
       title = {The Possibility of Measuring Thermal Protein Denaturation by an Optoacoustic Method.},
       booktitle = {Proc. SPIE},
       volume = {2077},
       pages = {202-212},
    
    }
    
  • Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon: Stray light rejection by structured illumination. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Peer2013,
       author = {Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon},
       title = {Stray light rejection by structured illumination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon: Speckle variance optical coherence tomography for imaging microcirculation. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Klawitter2013,
       author = {Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon},
       title = {Speckle variance optical coherence tomography for
    imaging microcirculation},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim: Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy. no. 8213, pp. 821338, SPIE,
    BibTeX
    @inproceedings{Ansari2012,
       author = {Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim},
       title = {Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821338},
    
    }
  • Hüttmann, G. and Hendrich, C. and Birngruber, R. and Lehnert, C. and Seara, J. and Siebert, W. E. and Diddens, H.: Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints . Mechanisms and Techniques in Photodynamic Therapy V,. in Proc. SPIE, no. 2675, pp. 238-242,
    BibTeX
    @inproceedings{Hüttmann,
       author = {Hüttmann, G. and Hendrich, C. and Birngruber, R. and Lehnert, C. and Seara, J. and Siebert, W. E. and Diddens, H.},
       title = {Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints . 
    Mechanisms and Techniques in Photodynamic Therapy V,},
       booktitle = {Proc. SPIE},
       volume = {2675},
       pages = {238-242},
    
    }
    
  • Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon: Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Fleischhauer2013,
       author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon},
       title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Brinkmann, R. and Huttmann, G. and Rogener, J. and Roider, J. and Birngruber, R. and Lin, C. P.: Origin of retinal pigment epithelium cell damage by pulsed laser irradiance in the nanosecond to microsecond time regimen. Lasers Surg Med, no. 27, pp. 451-64,
    BibTeX
    @article{Brinkmann2000,
       author = {Brinkmann, R. and Huttmann, G. and Rogener, J. and Roider, J. and Birngruber, R. and Lin, C. P.},
       title = {Origin of retinal pigment epithelium cell damage by pulsed laser irradiance in the nanosecond to microsecond time regimen},
       journal = {Lasers Surg Med},
       volume = {27},
       number = {5},
       pages = {451-64},
       note = {0196-8092 (Print)
    In Vitro
    Journal Article
    Research Support, Non-U.S. Gov't},
       abstract = {BACKGROUND AND OBJECTIVE: Selective photodamage of the retinal pigment epithelium (RPE) is a new technique to treat a variety of retinal diseases without causing adverse effects to surrounding tissues such as the neural retina including the photoreceptors and the choroid. In this study, the mechanism of cell damage after laser irradiation was investigated. STUDY DESIGN/MATERIALS AND METHODS: Single porcine RPE-melanosomes and RPE cells were irradiated with a Nd:YLF laser (wavelength lambda = 527 nm, adjustable pulse duration tau = 250 nsec-3 microsec) and a Nd:YAG laser (lambda = 532 nm, tau = 8 nsec). Fast flash photography was applied to observe vaporization at melanosomes in suspension. A fluorescence viability assay was used to probe the cells vitality. RESULTS: The threshold radiant exposures for vaporization around individual melanosomes and for ED50 cell damage are similar at 8-nsec pulse duration. Both thresholds increase with pulse duration; however, the ED50 cell damage radiant exposure is 40% lower at 3 microsec. Temperature calculations to model the onset of vaporization around the melanosomes are in good agreement with the experimental results when assuming a surface temperature of 150 degrees C to initiate vaporization and a homogeneous melanosome absorption coefficient of 8,000 cm(-1). Increasing the number of pulses delivered to RPE cells at a repetition rate of 500 Hz, the ED50 value 
  • Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.: OCT in the field of laryngology: further perspectives. no. 7883, pp. 78831W, SPIE,
    BibTeX
    @inproceedings{Just2011,
       author = {Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.},
       title = {OCT in the field of laryngology: further perspectives},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7883},
       pages = {78831W},
    
    }
  • Qu, Xiaochao and Norbert, Koop and Li, Zheng and Wang, Jing and Zhang, Zhenxi and Hüttmann, Gereon: Multiphoton fluorescence lifetime imaging of Karpas 299 cells using ACT1 antibody conjugated gold nanoparticles. no. 6630, pp. 66301C-66301C-8,
    BibTeX Link
    @inproceedings{Qu2007,
       author = {Qu, Xiaochao and Norbert, Koop and Li, Zheng and Wang, Jing and Zhang, Zhenxi and Hüttmann, Gereon},
       title = {Multiphoton fluorescence lifetime imaging of Karpas 299 cells using ACT1 antibody conjugated gold nanoparticles},
       volume = {6630},
       pages = {66301C-66301C-8},
       note = {10.1117/12.728239},
       abstract = {Due to the unique optical properties, gold nanoparticles (NPs) can play a useful role in biological cellular imaging as biological probes. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) system, we recorded the images of Karpas 299 cells incubated without, or with gold NPs, and ACT1 antibodies conjugated with gold NPs. From the FLIM, we can easily discriminate the difference among different experiment conditions due to the distinct lifetime between cells and gold NPs. Our results present that nonconjugated gold NPs are accumulated inside cells, but conjugated gold NPs bind homogeneously and specifically to the surface of cancer cells. For single Karpas 299 cells, the signal is very week when the excitation power is about 10mw; while the power is approximately 28 mw, a very sharp cell imaging can be obtained. For the Karpas 299 incubated with ACT1 conjugated gold NPs, while the excitation power is 10mw, gold NPs have clear fluorescence signal so that the profile of cells can be detected; Signal of gold NPs is very strong when the power arrived in 20mw. These results suggest that the multiphoton lifetime imaging of antibody conjugated gold NPs can support a useful method in diagnosis of cancer.},
       url = {http://dx.doi.org/10.1117/12.728239},
       type = {Conference Proceedings}
    }
    
  • Pan, Y. and Engelhardt, R. and Rosperich, J. and Hüttmann, G. and Birngruber, R.: Measurement of Optical-Transport-Coefficients of Intralipid in Visible and NIR Range. in Proc. SPIE, no. 2134A, pp. 354-363,
    BibTeX
    @inproceedings{Pan,
       author = {Pan, Y. and Engelhardt, R. and Rosperich, J. and Hüttmann, G. and Birngruber, R.},
       title = {Measurement of Optical-Transport-Coefficients of Intralipid in Visible and NIR Range},
       booktitle = {Proc. SPIE},
       volume = {2134A},
       pages = {354-363},
    
    }
    
  • Orzekowsky-Schroeder, Regina and Klinger, Antje and Schuth, Anna and Freidank, Sebastian and Huttmann, Gereon and Gebert, Andreas and Vogel, Alfred: Intravital real-time study of tissue response to controlled laser-induced cavitation using 500-ps UV laser pulses focused in murine gut mucosa under online dosimetry and spectrally resolved 2-photon microscopy. no. 7568, pp. 756815, SPIE,
    BibTeX
    @inproceedings{Orzekowsky2010,
       author = {Orzekowsky-Schroeder, Regina and Klinger, Antje and Schuth, Anna and Freidank, Sebastian and Huttmann, Gereon and Gebert, Andreas and Vogel, Alfred},
       title = {Intravital real-time study of tissue response to controlled laser-induced cavitation using 500-ps UV laser pulses focused in murine gut mucosa under online dosimetry and spectrally resolved 2-photon microscopy},
       editor = {Daniel, L. Farkas and Dan, V. Nicolau and Robert, C. Leif},
       publisher = {SPIE},
       volume = {7568},
       pages = {756815},
    
    }
  • Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: Imaging vascular dynamics in human retina using full-field swept-source optical coherence tomography (Conference Presentation). no. 9697, pp. 96970E-96970E-1,
    BibTeX Link
    @inproceedings{Spahr2016,
       author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {Imaging vascular dynamics in human retina using full-field swept-source optical coherence tomography (Conference Presentation)},
       volume = {9697},
       pages = {96970E-96970E-1},
       note = {10.1117/12.2214303},
       abstract = {We demonstrate a new non-invasive method to assess the functional condition of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. Motion of retinal tissue, that is induced by expansion of the vessels therein, is measured with an accuracy of about 10 nm. The pulse shape of arterial and venous pulsation, their temporal delay as well as the frequency dependent pulse propagation through the capillary bed are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
       url = {http://dx.doi.org/10.1117/12.2214303},
       type = {Conference Proceedings}
    }
    
  • Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon: Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT. no. 7889, pp. 78890E, SPIE,
    BibTeX
    @inproceedings{Müller2011,
       author = {Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon},
       title = {Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78890E},
    
    }
  • Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon: Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography. no. 8213, pp. 82131S, SPIE,
    BibTeX
    @inproceedings{Spahr2012,
       author = {Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon},
       title = {Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131S},
    
    }
  • Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter: Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation). no. 9691, pp. 969116-969116-1,
    BibTeX Link
    @inproceedings{Pieper2016,
       author = {Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter},
       title = {Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation)},
       volume = {9691},
       pages = {969116-969116-1},
       note = {10.1117/12.2209054},
       abstract = {Mucus transport is essential to remove inhaled particles and pathogens from the lung. Impaired removal of mucus often results in worsening of lung diseases. To understand the mechanisms of mucus transport and to monitor the impact of therapeutic strategies, it is essential to visualize airways and mucus in living animals without disturbing transport processes by intubation or surgically opening the airways. We developed a custom-built optical coherence microscope (OCM) providing a lateral and axial resolution of approximately 1.5 µm with a field of view of 2 mm at up to 150 images/s. Images of the intact trachea and its mucus transport were recorded in anesthetized spontaneously breathing mice. NaCl solution (0.9% and 7%) or Lipopolysaccharide were applied intranasally. OCM resolved detailed structure of the trachea and enabled measuring the airway surface liquid (ASL) thickness through the tracheal wall. Without stimulation, the amount of ASL was only a few µm above the epithelium and remained constant. After intranasal application of 30 µl saline at different concentrations, an early fast cough-like fluid removal with velocities higher than 1 mm/s was observed that removed a high amount of liquid. The ASL thickness increased transiently and quickly returned to levels before stimulation. In contrast to saline, application of Lipopolysaccharide induced substantial mucus release and an additional slow mucus transport by ciliary beating (around 100 µm/s) towards the larynx was observed. In conclusion, OCM is appropriate unique tool to study mechanisms of mucus transport in the airways and effects of therapeutic interventions in living animals.},
       url = {http://dx.doi.org/10.1117/12.2209054},
       type = {Conference Proceedings}
    }
    
  • Hüttmann, G. and Orzekowsky-Schröder, R.: Imaging of immune cell dynamics in small intestine and the eye by 2-photon microcopy. in 7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012),
    BibTeX
    @inproceedings{Hüttmann2012,
       author = {Hüttmann, G. and Orzekowsky-Schröder, R.},
       title = {Imaging of immune cell dynamics in small intestine and the eye  by 2-photon microcopy},
       booktitle = {7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012)},
       abstract = {Autofluorescence based 2-photon microscopy was investigated as a
    tool for studying the dynamics of immunological processes in vivo. Nearly
    all tissue components were simultaneously visible by autofluorescence and a
    nearly complete visualization of tissue architecture was possible. Within
    the tissue, immune competent cells like lymphocytes, macrophages and
    dendritic cells were visualized in their dynamic interaction with other
    cells or surrounding tissues.
    Immunological processes were studied in the small intestine and at the
    surface of the eye. Excitation and emission spectra of the different mucosal
    tissue components were quantitatively determined and a compared to the
    respective spectra of endogenous chromophores. It was shown, that by using
    only two excitation wavelengths within the tuning range of a Ti:Saphire
    laser enterocytes, antigen presenting cells and lysosomes of the small
    intestine could be discriminated based on the excitation and emission
    properties. By additionally using an intravital nuclear stain, motion of
    lymphocytes in the lamina propria and the epithelium of small intestine
    villi was quantitatively analyzed. 
    
    2-photon microscopy was also a powerful tool for studying the conjunctiva
    and cornea of the eye. Lymphocyte dynamics and uptake of microspheres or
    fluorescing heat-inactivated E. coli was followed over time in the
    conjunctiva-associated lymphoid tissue of mice. In a different mouse model
    of suture induced corneal hem- and lymphangiogenesis, immune cell migration
    into lymphatic vessels and luminal transport of individual cells was
    observed in vivo.
    
    Autofluorescence based 2-photon intravital microscopy is a valuable tool to
    study dynamic immunological processes. While tissue autofluorescence gives a
    good overview over all relevant structures and allows for discriminating
    different cell types by spectral analysis, the additional combination of
    specific staining with external dyes or fluorescent proteins is easily
    possible and enhances the potential of the technique further. },
    
    }
  • Hillmann, Dierck and Luhrs, Christian and Bonin, Tim and Koch, Peter and Vogel, Alfred and Huttmann, Gereon: Holoscopy: holographic optical coherence tomography. no. 8091, pp. 80911H, Proc. SPIE,
    BibTeX Link
    @inproceedings{Hillmann2011,
       author = {Hillmann, Dierck and Luhrs, Christian  and Bonin, Tim  and Koch, Peter and Vogel, Alfred  and Huttmann, Gereon },
       title = {Holoscopy: holographic optical coherence tomography},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {Proc. SPIE},
       volume = {8091},
       pages = {80911H},
       url = {http://link.aip.org/link/?PSI/8091/80911H/1
    http://dx.doi.org/10.1117/12.889485},
       type = {Conference Proceedings}
    }
    
  • Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon: High resolution holoscopy. no. 8213, pp. 821324, SPIE,
    BibTeX
    @inproceedings{Franke2012,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon},
       title = {High resolution holoscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821324},
    
    }
  • Hüttmann, G. and Achtelik, W. and Löning, M. and Sommer, K. and Diddens, H.: Fluorescence microscopy studies on ALA sensitized tissues. in SPIE Proc., no. 2926, pp. 24-31,
    BibTeX
    @inproceedings{Hüttmann,
       author = {Hüttmann, G. and Achtelik, W. and Löning, M. and Sommer, K. and Diddens, H.},
       title = {Fluorescence microscopy studies on ALA sensitized tissues},
       booktitle = {SPIE Proc.},
       volume = {2926},
       pages = {24-31},
    
    }
    
  • Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.: Femtosecond laser nanosurgery of biological cells and tissues. in 4th International Congress on Laser Advanced Materials Processing,
    BibTeX
    @inproceedings{Vogel2006,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Femtosecond laser nanosurgery of biological cells and tissues},
       booktitle = {4th International Congress on Laser Advanced Materials Processing}
    }
  • Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander: Feasibiliy of optical detection of soft tissue deformation during needle insertion. no. 8316, pp. 83160V, SPIE,
    BibTeX
    @inproceedings{Otte,
       author = {Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander},
       title = {Feasibiliy of optical detection of soft tissue deformation during needle insertion},
       editor = {David, R. Holmes, III and Kenneth, H. Wong},
       publisher = {SPIE},
       volume = {8316},
       pages = {83160V},
    
    }
  • Daniltchenko, D. and Sachs, M. and Lankenau, E. and König, F. and Burckhardt, M. and Hüttmann, G. and Kristiansen, G. and Schnorr, D. and Al-Shukri, S. and Löning, S.: Ex vivo and in vivo topographics studies of bladder by optical coherence tomography. in Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, no. 5771, pp. 209-14, Proceedings 2005,
    BibTeX
    @inproceedings{Daniltchenko,
       author = {Daniltchenko, D. and Sachs, M. and Lankenau, E. and König, F. and Burckhardt, M. and Hüttmann, G. and Kristiansen, G. and Schnorr, D. and Al-Shukri, S. and Löning, S.},
       title = {Ex vivo and in vivo topographics studies of bladder by optical coherence tomography},
       booktitle = {Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI},
       editor = {Tuchin, V.},
       publisher = {Proceedings 2005},
       volume = {5771},
       pages = {209-14},
    
    }
  • Lankenau, E. and Schumacher, M. and Koch, P. and König, F. and Daniltchenko, D. and Schmorr, D. and Hüttmann, G.: Dispersion compensation for proximal scanning rigid OCT endoscopes. in SPIE Proc., no. 5316, pp. 172-177,
    BibTeX
    @inproceedings{Lankenau,
       author = {Lankenau, E. and Schumacher, M. and Koch, P. and König, F. and Daniltchenko, D. and Schmorr, D. and Hüttmann, G.},
       title = {Dispersion compensation for proximal scanning rigid OCT endoscopes},
       booktitle = {SPIE Proc.},
       volume = {5316},
       pages = {172-177},
    
    }
  • Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.: Diffusion-sensitive Fourier-domain optical coherence tomography. no. 7889, pp. 78892B, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert2011,
       author = {Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.},
       title = {Diffusion-sensitive Fourier-domain optical coherence tomography},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78892B},
    
    }
  • Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.: Diffusion-sensitive Fourier-domain optical coherence tomography. no. 7889, pp. 78892B, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert2011,
       author = {Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.},
       title = {Diffusion-sensitive Fourier-domain optical coherence tomography},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78892B},
    
    }
  • Steven, Philip and Koop, Norbert and Huttmann, Gereon: Confocal microscopy versus two-photon microscopy: imaging of ocular surface pathologies. no. 6860, pp. 686023, SPIE,
    BibTeX
    @inproceedings{Steven,
       author = {Steven, Philip and Koop, Norbert and Huttmann, Gereon},
       title = {Confocal microscopy versus two-photon microscopy: imaging of ocular surface pathologies},
       editor = {Ammasi, Periasamy and Peter, T. C. So},
       publisher = {SPIE},
       volume = {6860},
       pages = {686023},
    
    }
  • Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.: Confocal endomicroscopy of the larynx. no. 8207, pp. 82072H, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.},
       title = {Confocal endomicroscopy of the larynx},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian Jet-Fei, Wong and Justus, F. Ilgner and Krzysztof, Izdebski and Melissa, J. Suter and Stephen, Lam and Matthew, Brenner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, Madsen and Anita, Mahadevan-Jansen and Jansen, E. Duco and Andreas, Mandelis},
       publisher = {SPIE},
       volume = {8207},
       pages = {82072H},
    
    }
  • Bonin, Tim and Koch, Peter and Huttmann, Gereon: Comparison of fast swept source full-field OCT with conventional scanning OCT. no. 8091, pp. 80911K, SPIE,
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Koch, Peter and Huttmann, Gereon},
       title = {Comparison of fast swept source full-field OCT with conventional scanning OCT},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {SPIE},
       volume = {8091},
       pages = {80911K},
    
    }
  • Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Analysis of the signal fall-off in spectral domain optical coherence tomography systems. no. 8213, pp. 82131K, SPIE,
    BibTeX
    @inproceedings{Hagen-Eggert,
       author = {Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {Analysis of the signal fall-off in spectral domain optical coherence tomography systems},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131K},
    
    }
    
  • Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.: An optical coherence tomography study for imaging the round window niche and the promontorium tympani. no. 7548, pp. 754833, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.},
       title = {An optical coherence tomography study for imaging the round window niche and the promontorium tympani},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Reza, S. Malek and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7548},
       pages = {754833},
    
    }
  • Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.: An optical coherence tomography study for imaging the round window niche and the promontorium tympani. no. 7548, pp. 754833, SPIE,
    BibTeX
    @inproceedings{Just,
       author = {Just, T. and Lankenau, E. and Huttmann, G. and Pau, H. W.},
       title = {An optical coherence tomography study for imaging the round window niche and the promontorium tympani},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Reza, S. Malek and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7548},
       pages = {754833},
    
    }
  • Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K: An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Sudkamp2013,
       author = {Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K},
       title = {An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}