@article{Klinger2017,
   author = {Klinger, A. and Krapf, L. and Orzekowsky-Schroeder, R. and Koop, N. and Vogel, A. and Huttmann, G.},
   title = {Intravital autofluorescence 2-photon microscopy of murine intestinal mucosa with ultra-broadband femtosecond laser pulse excitation: image quality, photodamage, and inflammation},
   journal = {J Biomed Opt},
   volume = {20},
   number = {11},
   pages = {116001},
   ISSN = {1083-3668},
   DOI = {10.1117/1.jbo.20.11.116001},
   year = {2015},
   type = {Journal Article}
}

@article{Hüttmann2015,
   author = {Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald},
   title = {Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {56},
   number = {7},
   pages = {5980-5980},
   ISSN = {1552-5783},
   year = {2015},
   type = {Journal Article}
}

@article{Schulz-Hildebrandt2015,
   author = {Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.},
   title = {Improved endoscopic optical coherence microscopy for imaging of humans airways in patients},
   journal = {Pneumologie},
   volume = {69},
   number = {07},
   pages = {A49},
   ISSN = {0934-8387},
   DOI = {10.1055/s-0035-1556641},
   year = {2015},
   type = {Journal Article}
}

@article{Spahr2015,
   author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
   title = {Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography},
   journal = {Optics Letters},
   volume = {40},
   number = {20},
   pages = {4771-4774},
   abstract = {We demonstrate a new noninvasive method to assess biomechanical properties of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. The motion of retinal tissue that is induced by expansion of the vessels therein is measured with an accuracy of about 10 nm. The pulse shapes of arterial and venous pulsations, their temporal delays, as well as the frequency-dependent pulse propagation through the capillary bed, are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
   keywords = {Optical coherence tomography
Ophthalmology
Time-resolved imaging
Functional monitoring and imaging},
   DOI = {10.1364/OL.40.004771},
   url = {http://ol.osa.org/abstract.cfm?URI=ol-40-20-4771},
   year = {2015},
   type = {Journal Article}
}

@article{Spahr2015,
   author = {Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Hillmann, Dierck and Huttmann, Gereon},
   title = {Functional Microangiography of in vivo human retina by Full-Field OCT},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {56},
   number = {7},
   pages = {5974-5974},
   abstract = { PurposeOCT based functional microangiography of the retina requires high speed acquisition of a large number of volumetric datasets. Imaging speed of conventional scanning OCT devices is limited by the applicable radiant power and the mechanics used to scan the focused beam over the desired field of view. Full-Field Swept-Source OCT (FF-SS-OCT) resolves both issues, using an areal illumination, which dramatically increases the allowed amount of radiation, and an ultrafast camera for a highly parallelized acquisition.  MethodsThe retina of healthy volunteers was illuminated with wavelengths between 816 and 867 nm by the extended beam of a tunable laser (Broadsweeper, Superlum). Retinal irradiance was below the maximum permissable exposure (MPE). Light backscattered from the retina was imaged onto an ultrafast CMOS camera (SA-Z, Photron), where it interfered with an extended reference beam. From a series of interference images at different wavelengths, volumetric OCT images of the retina were reconstructed.  ResultsWe demonstrate in vivo retinal imaging at 9.9 billion voxels per second (40 million A-scans/s with 256 axial pixels). Sacrificing depth resolution by reducing the number of axial pixels, the A-scan rate was increased to more than 1 billion A-scans per second. FF-SS-OCT allowed imaging of all important retinal structures with good quality at unprecedented imaging speed (see fig. 1). Fast volumetric imaging at up to 3000 volumes/s was used to visualize small capillaries and to analyze the pulsation of retinal arteries and veins (see fig. 2). Imaging time for an area of 4 mm x 2 mm (896 x 368 A-scans) was only 316 µs. The high volume rate and the inherent phase stability enabled quantitative measurement of the change of retinal thickness due to blood pulsation with approx. 10 nm precision. A delay of the venous pulsation with respect to the arteries was observed (approx. 11 ms). The amplitudes of higher frequency components of the venous pulsation were considerably attenuated.  ConclusionsFF-SS-OCT provides fast volumetric imaging of the retina with good image quality. The capillary network can be analyzed with high spatial and temporal resolution. Analysis of retinal pulsation may provide information on pathological changes of vessels and capillaries. Angiographic OCT acquired with the FF-SS-OCT setup. Functional angiography showing the pulsation of retinal artery and vein.},
   ISSN = {1552-5783},
   url = {http://dx.doi.org/},
   year = {2015},
   type = {Journal Article}
}

@article {Steven900,
	author = {Philipp Steven and Carolin Le Blanc and Eva Lankenau and Marc Krug and Stefan Oelckers and Ludwig M Heindl and Uta Gehlsen and Gereon Huettmann and Claus Cursiefen},
	title = {Optimising deep anterior lamellar keratoplasty (DALK) using intraoperative online optical coherence tomography (iOCT)},
	volume = {98},
	number = {7},
	pages = {900--904},
	year = {2014},
	doi = {10.1136/bjophthalmol-2013-304585},
	publisher = {BMJ Publishing Group Ltd},
	abstract = {Background/aims To describe the use of intraoperative online optical coherence tomography (iOCT) for improving deep anterior lamellar keratoplasty (DALK) surgery. Methods Retrospective case series of 6 eyes of 6 male patients with keratokonus, corneal dystrophy or herpetic stromal scars undergoing DALK were investigated using intraoperative optical coherence tomography and postsurgical image/video analysis. Main outcome measures were: visibility of surgical steps, especially, assessment of placement depth of injection needle, preparation of bare Descemet{\textquoteright}s membrane and drainage of interface fluid. Results iOCT enables real-time visualisation of all surgical steps of DALK procedure in all patients. Placement of air injection needle above Descemet{\textquoteright}s membrane was reliably monitored as was presence of bare Descemet{\textquoteright}s membrane and potential interface fluid. Conclusions iOCT assists with visualisation of injection needle placement and with assessment of bare Descemet{\textquoteright}s membrane as well as interface fluid during the DALK procedure. Overall iOCT may be a helpful device that supports surgeons in all steps of DALK procedure.},
	issn = {0007-1161},
	URL = {https://bjo.bmj.com/content/98/7/900},
	eprint = {https://bjo.bmj.com/content/98/7/900.full.pdf},
	journal = {British Journal of Ophthalmology}
}

@article{Guder2014,
   author = {Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino},
   title = {Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography},
   journal = {European Archives of Oto-Rhino-Laryngology},
   pages = {1-7},
   keywords = {Optical coherence tomography
Tympanic membrane
Chronic myringitis},
   ISSN = {0937-4477},
   DOI = {10.1007/s00405-014-3373-z},
   url = {http://dx.doi.org/10.1007/s00405-014-3373-z},
   year = {2014},
   type = {Journal Article}
}

@article{Ansari2014,
   author = {Ansari, Rehman and Myrtus, Christian and Aherrahrou, Redouane and Erdmann, Jeanette and Schweikard, Achim and Hüttmann, Gereon},
   title = {Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy},
   journal = {Optics Letters},
   volume = {39},
   number = {1},
   pages = {45-47},
   abstract = {We present an ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy (SD-OCPM) system that combines submicrometer transverse spatial resolution and subnanometer optical path length sensitivity, with an acquisition speed of over 217,000  voxels/s. The proposed SD-OCPM system overcomes two significant drawbacks of traditional common-path interferometers—limited transverse spatial resolution and suboptimal detection sensitivity—while maintaining phase stability that is comparable with common-path interferometer setups. The transverse and axial spatial resolution of the setup is measured to be 0.6 and 1.9 μm, respectively, with a phase sensitivity of 0.0027 rad (corresponds to optical path length sensitivity of 110 pm). High-speed acquisition allows for phase-sensitive 4D imaging of biological samples with subcellular resolution.},
   keywords = {Microscopy
Coherence imaging
Three-dimensional microscopy},
   DOI = {10.1364/OL.39.000045},
   url = {http://ol.osa.org/abstract.cfm?URI=ol-39-1-45},
   year = {2014},
   type = {Journal Article}
}

@article{Xie_2013,
doi = {10.1088/0031-9155/58/3/555},
url = {https://dx.doi.org/10.1088/0031-9155/58/3/555},
year = {2013},
month = {jan},
publisher = {IOP Publishing},
volume = {58},
number = {3},
pages = {555},
author = {Yijing Xie and Tim Bonin and Susanne Löffler and Gereon Hüttmann and Volker Tronnier and Ulrich G Hofmann},
title = {Coronal in vivo forward-imaging of rat brain morphology with an ultra-small optical coherence tomography fiber probe},
journal = {Physics in Medicine & Biology},
abstract = {A well-established navigation method is one of the key conditions for successful brain surgery: it should be accurate, safe and online operable. Recent research shows that optical coherence tomography (OCT) is a potential solution for this application by providing a high resolution and small probe dimension. In this study a fiber-based spectral-domain OCT system utilizing a super-luminescent-diode with the center wavelength of 840 nm providing 14.5 μm axial resolution was used. A composite 125 μm diameter detecting probe with a gradient index (GRIN) fiber fused to a single mode fiber was employed. Signals were reconstructed into grayscale images by horizontally aligning A-scans from the same trajectory with different depths. The reconstructed images can display brain morphology along the entire trajectory. For scans of typical white matter, the signals showed a higher reflection of light intensity with lower penetration depth as well as a steeper attenuation rate compared to the scans typical for gray matter. Micro-structures such as axon bundles (70 μm) in the caudate nucleus are visible in the reconstructed images. This study explores the potential of OCT to be a navigation modality in brain surgery.}
}

@article{10.1001/jamaophthalmol.2013.4672,
    author = {Steven, Philipp and Le Blanc, Carolin and Velten, Kai and Lankenau, Eva and Krug, Marc and Oelckers, Stefan and Heindl, Ludwig M. and Gehlsen, Uta and Hüttmann, Gereon and Cursiefen, Claus},
    title = "{Optimizing Descemet Membrane Endothelial Keratoplasty Using Intraoperative Optical Coherence Tomography}",
    journal = {JAMA Ophthalmology},
    volume = {131},
    number = {9},
    pages = {1135-1142},
    year = {2013},
    month = {09},
    abstract = "{Descemet membrane endothelial keratoplasty (DMEK) is a challenging procedure for the surgeon, particularly because of deficient visibility of the delicate tissue due to the natural en face view through the operating microscope. A cross-sectional view would greatly enhance intraoperative overview and enable the surgeon to better control the procedure.  To retrospectively analyze the use of intraoperative optical coherence tomography (iOCT) for improving the safety of DMEK.Intraoperative OCT during DMEK was performed in 26 eyes of 26 patients. We retrospectively analyzed imaging and video data. Department of Ophthalmology, University of Cologne.Seven men and 19 women aged 39 to 93 years with corneal endothelial dysfunction undergoing DMEK.Descemet membrane endothelial keratoplasty.Visibility of surgical steps, overall duration of DMEK, overall time for complete intraoperative air filling of the anterior chamber, and correlation between donor age and Descemet rolling behavior.Intraoperative OCT enables visualization of all steps of the DMEK procedure. Overall mean (SD) duration of the DMEK procedure was 25.7 (6.9) minutes when using iOCT. Overall mean (SD) complete intraoperative anterior chamber air-filling time was 236 (108) seconds in contrast to 60 to 90 minutes for standard air-filling time. Descemet membrane rolling behavior showed significant inverse correlation between donor age (range, 39-93 years) and the extent of rolling (R2 = 0.5 [P = .006]).Intraoperative OCT enhances the visibility of graft orientation and unfolding, thereby improving safety of the DMEK procedure. Overall, iOCT is a helpful device that may support surgeons in all steps of DMEK procedures.}",
    issn = {2168-6165},
    doi = {10.1001/jamaophthalmol.2013.4672},
    url = {https://doi.org/10.1001/jamaophthalmol.2013.4672},
    eprint = {https://jamanetwork.com/journals/jamaophthalmology/articlepdf/1708786/eoi130143.pdf},
}

@article{LankenauKrugOelckersSchrageJustHüttmann+2013+233+239,
url = {https://doi.org/10.1515/aot-2013-0011},
title = {iOCT with surgical microscopes: a new imaging during microsurgery},
title = {},
author = {Eva Maria Lankenau and Marc Krug and Stefan Oelckers and Norbert Schrage and Tino Just and Gereon Hüttmann},
pages = {233--239},
volume = {2},
number = {3},
journal = {Advanced Optical Technologies},
doi = {doi:10.1515/aot-2013-0011},
year = {2013},
lastchecked = {2023-04-13}
}

@inproceedings{10.1117/12.2033174,
author = {B. Olzowy and N. Starke and T. Schuldt and G. H{\"u}ttmann and E. Lankenau and T. Just},
title = {{Optical coherence tomography and confocal endomicroscopy for rhinologic pathologies: a pilot study}},
volume = {8805},
booktitle = {Head and Neck Optical Diagnostics},
editor = {Christian Betz and Brian J. F. Wong M.D.},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {880505},
keywords = {optical coherence tomography, confocal microscopy, inverted papilloma , chronic rhinosinusitis, nasal polyps, mucociliary function, ciliated epithelium},
year = {2013},
doi = {10.1117/12.2033174},
URL = {https://doi.org/10.1117/12.2033174}
}

@inproceedings{Fleischhauer2013,
   author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon},
   title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination},
   booktitle = {Studierendentagung},
   publisher = {Universität zu Lübeck},
   type = {Conference Proceedings},
url = { https://pdfs.semanticscholar.org/a581/a18366acff021e12dcc090b40890ea70dcb8.pdf},
 year = { 2013}
}

@misc{Miura2013,
   author = {Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R },
   title = {Two-photon Microscopy and Fluorescence Lifetime Analysis of Lipid Peroxidation Product in Photoreceptor Outer Segment and in Retinal Pigment Epithelial Cell},
   publisher = {ARVO Meeting Abstracts},
   month = {March 26, 2012 },
   url = {http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=57630548-893d-4e45-9ddc-b6f547dd4ff0&cKey=d08a30bc-fe98-40a2-8a1c-1b171e4becd3&mKey=f0fce029-9bf8-4e7c-b48e-9ff7711d4a0e},
   year = {2013},
   type = {Poster}
}

@article{Franke2013,
   author = {Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon},
   title = {Towards microscopic resolution in holoscopy},
   pages = {85711O-85711O},
   note = {10.1117/12.2006806},
   abstract = {Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.},
   DOI = {10.1117/12.2006806},
   url = {http://dx.doi.org/10.1117/12.2006806},
   year = {2013},
   type = {Journal Article}
}

@inproceedings{Sudkamp2013,
   author = {Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K},
   title = {An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array},
   booktitle = {Studierendentagung},
   publisher = {Universität zu Lübeck},
   type = {Conference Proceedings},
year= { 2013}
}

@article{Hillmann2013,
   author = {Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon},
   title = {Off-axis full-field swept-source optical coherence tomography using holographic refocusing},
   pages = {857104-857104},
   note = {10.1117/12.2006436},
   abstract = {We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.},
   DOI = {10.1117/12.2006436},
   url = {http://dx.doi.org/10.1117/12.2006436},
   year = {2013},
   type = {Journal Article}
}

@article{Yao,
   author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
   title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
   journal = {J Biomed Opt},
   volume = {14},
   pages = {054034},
   note = {Journal article},
   year = {2009}
}

@article{Yao,
   author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.},
   title = {Laser-based gene transfection and gene therapy},
   journal = {IEEE Trans Nanobioscience},
   volume = {7},
   number = {2},
   pages = {111-9},
   note = {Yao, C P
Zhang, Z X
Rahmanzadeh, R
Huettmann, G
Research Support, Non-U.S. Gov't
Review
United States
IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.},
   abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.},
   keywords = {Cell Membrane/ radiation effects
DNA/ administration & dosage/ pharmacokinetics
Gene Therapy/ methods
Lasers
Transfection/ methods},
   year = {2008}
}

@misc{Qu,
   author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.},
   title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes},
   volume = {13},
   number = {3},
   pages = {031217},
   note = {Using Smart Source Parsing
May-Jun},
   abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.},
   ISBN = {1083-3668 (Print)
1083-3668 (Linking)},
   year = {2008}
}

@article{Rahmanzadeh,
   author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.},
   title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis},
   journal = {Cell Prolif},
   volume = {40},
   number = {3},
   pages = {422-30},
   note = {Rahmanzadeh, R
Huttmann, G
Gerdes, J
Scholzen, T
England
Cell Prolif. 2007 Jun;40(3):422-30.},
   abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.},
   keywords = {Antibodies, Antinuclear
Antibodies, Monoclonal
Cell Division/physiology
Cell Nucleolus/physiology
Fluorescein-5-isothiocyanate
Fluorescent Dyes
HeLa Cells
Humans
Ki-67 Antigen/*genetics/*metabolism
Photochemistry
RNA Polymerase I/metabolism
RNA, Ribosomal/*biosynthesis},
   year = {2007}
}

@article{Yao,
   author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.},
   title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles},
   journal = {J Biomed Opt},
   volume = {10},
   number = {6},
   pages = {064012},
   note = {Yao, Cuiping
Rahmanzadeh, Ramtin
Endl, Elmar
Zhang, Zhenxi
Gerdes, Johannes
Huttmann, Gereon
Research Support, Non-U.S. Gov't
United States
J Biomed Opt. 2005 Nov-Dec;10(6):064012.},
   abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.},
   keywords = {Biopolymers/pharmacokinetics
Cell Line, Tumor
Cell Membrane Permeability/ physiology/ radiation effects
Drug Delivery Systems/ methods
Fluoresceins/ pharmacokinetics
Humans
Lasers
Lymphoma/ metabolism
Nanostructures},
   year = {2005}
}

@inproceedings{Radt-2001,
   author = {Radt, Benno and Serbin, Jesper and Lange, Bjoern I. and Birngruber, Reginald and Huettmann, Gereon},
   title = {Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses},
   editor = {Reginald, Birngruber and Hubert van den, Bergh},
   publisher = {SPIE},
   volume = {4433},
   pages = {16-24},
year = { 2001},
URL = { https://doi.org/10.1117/12.446518}

}

@inproceedings{Lange2001,
   author = {Lange, Bjoern I. and Radt, Benno and Huettmann, Gereon},
   title = {Cr,Tm,Ho: YAG laser amplifier},
   editor = {Richard, Scheps},
   publisher = {SPIE},
   volume = {4267},
   pages = {169-174},
URL = {https://doi.org/10.1117/12.424616},
Year = { 2001}
}

@article{Hüttmann,
   author = {Hüttmann, G. and Serbin, J. and Radt, B. and Lange, Björn I. and Birngruber, R.},
   title = {Model system for investigating laser-induced subcellular microeffects.},
   journal = {Proc SPIE},
   volume = {4257},
   pages = {398-409},
   year = {2001}
}

@article{Hüttmann,
   author = {Hüttmann, G. and Birngruber, R.},
   title = {Laserinduzierte thermische Gewebseffekte mit mikroskopischer und makromolekularer Präzision},
   journal = {Z Med Phys},
   volume = {10},
   pages = {169-174},
   year = {2000}
}

@article{Hüttmann,
   author = {Hüttmann, G. and Radt, B. and Birngruber, R.},
   title = {Laserinduzierte Mikro- und Nanoeffekte - Von der selektiven Thermolyse zu molekularen Nanoeffekten},
   journal = {LaserOpto},
   volume = {32},
   pages = {47-55},
   year = {2000}
}

@article{Brinkmann2000-1,
   author = {Brinkmann, R. and Huttmann, G. and Rogener, J. and Roider, J. and Birngruber, R. and Lin, C. P.},
   title = {Origin of retinal pigment epithelium cell damage by pulsed laser irradiance in the nanosecond to microsecond time regimen},
   journal = {Lasers Surg Med},
   volume = {27(5)},
   Year = { 2000},
url = { https://www.researchgate.net/publication/227934019_Origin_of_retinal_pigment_epithelium_cell_damage_by_pulsed_laser_irradiance_in_the_nanosecond_to_microsecond_time_regimen},
   pages = {451-64},
   note = {0196-8092 (Print)
In Vitro
Journal Article
Research Support, Non-U.S. Gov't},
   abstract = {BACKGROUND AND OBJECTIVE: Selective photodamage of the retinal pigment epithelium (RPE) is a new technique to treat a variety of retinal diseases without causing adverse effects to surrounding tissues such as the neural retina including the photoreceptors and the choroid. In this study, the mechanism of cell damage after laser irradiation was investigated. STUDY DESIGN/MATERIALS AND METHODS: Single porcine RPE-melanosomes and RPE cells were irradiated with a Nd:YLF laser (wavelength lambda = 527 nm, adjustable pulse duration tau = 250 nsec-3 microsec) and a Nd:YAG laser (lambda = 532 nm, tau = 8 nsec). Fast flash photography was applied to observe vaporization at melanosomes in suspension. A fluorescence viability assay was used to probe the cells vitality. RESULTS: The threshold radiant exposures for vaporization around individual melanosomes and for ED50 cell damage are similar at 8-nsec pulse duration. Both thresholds increase with pulse duration; however, the ED50 cell damage radiant exposure is 40% lower at 3 microsec. Temperature calculations to model the onset of vaporization around the melanosomes are in good agreement with the experimental results when assuming a surface temperature of 150 degrees C to initiate vaporization and a homogeneous melanosome absorption coefficient of 8,000 cm(-1). Increasing the number of pulses delivered to RPE cells at a repetition rate of 500 Hz, the ED50 value }
}

@article{Schüle,
   author = {Schüle, G. and Hüttmann, G. and Roider, J. and Wirbelauer, C. and Birngruber, R. and Brinkmann, R.},
   title = {Optoacoustic measurements during µs-irradiation of the retinal pigment epithelium},
   journal = {Proc. SPIE},
   volume = {3914A},
   year = {2000}
}

@article{Brinkmann1999,
   author = {Brinkmann, R. and Rögener, J. and Lin, C.P. and Roider, J. and Birngruber, R. and Hüttmann, G.},
   title = {Selective RPE-Photodestruction: Mechanism of Cell Damage by pulsed laser irradiance in the ns to µs time regime},
   journal = {Proc. SPIE},
   volume = {3601},
   pages = {59-65},
   year = { 1999}
}