@article{Yao,
   author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.},
   title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles},
   journal = {J Biomed Opt},
   volume = {10},
   number = {6},
   pages = {064012},
   note = {Yao, Cuiping
Rahmanzadeh, Ramtin
Endl, Elmar
Zhang, Zhenxi
Gerdes, Johannes
Huttmann, Gereon
Research Support, Non-U.S. Gov't
United States
J Biomed Opt. 2005 Nov-Dec;10(6):064012.},
   abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.},
   keywords = {Biopolymers/pharmacokinetics
Cell Line, Tumor
Cell Membrane Permeability/ physiology/ radiation effects
Drug Delivery Systems/ methods
Fluoresceins/ pharmacokinetics
Humans
Lasers
Lymphoma/ metabolism
Nanostructures},
   year = {2005}
}

@inproceedings{Koch-2004,
   author = {Koch, Peter and Huettmann, Gereon and Schleiermacher, Hansfrieder and Eicholz, Joerg and Koch, Edmund},
   title = {Linear OCT system with down conversion of the fringe pattern},
   editor = {Valery, V. Tuchin and Joseph, A. Izatt and James, G. Fujimoto},
   publisher = {SPIE},
   volume = {5316},
   pages = {260-267},
Year = { 2004},
URL = { https://doi.org/10.1117/12.531323}

}

@inproceedings{Hüttmann2003,
   author = {Huettmann, Gereon and Radt, Benno and Serbin, Jesper and Birngruber, Reginald},
   title = {Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses},
   editor = {Rudolf, W. Steiner},
   publisher = {SPIE},
   volume = {5142},
   pages = {88-95},
URL = { https://doi.org/10.1364/ECBO.2003.5142_88},
year = { 2003}
}

@inproceedings{Schuele-2002,
   author = {Schuele, Georg and Huettmann, Gereon and Brinkmann, Ralf},
   title = {Noninvasive temperature measurements during laser irradiation of the retina with optoacoustic techniques},
   editor = {Fabrice, Manns and Per, G. Soederberg and Arthur, Ho},
   publisher = {Proc. SPIE},
   volume = {4611},
   pages = {64-71},
year = { 2002},
url = { https://doi.org/10.1117/12.470601} 
}

@inproceedings{Lange2001,
   author = {Lange, Bjoern I. and Radt, Benno and Huettmann, Gereon},
   title = {Cr,Tm,Ho: YAG laser amplifier},
   editor = {Richard, Scheps},
   publisher = {SPIE},
   volume = {4267},
   pages = {169-174},
URL = {https://doi.org/10.1117/12.424616},
Year = { 2001}
}

@inproceedings{Radt-2001,
   author = {Radt, Benno and Serbin, Jesper and Lange, Bjoern I. and Birngruber, Reginald and Huettmann, Gereon},
   title = {Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses},
   editor = {Reginald, Birngruber and Hubert van den, Bergh},
   publisher = {SPIE},
   volume = {4433},
   pages = {16-24},
year = { 2001},
URL = { https://doi.org/10.1117/12.446518}

}

@article{Eichenauer,
   author = {Eichenauer, Rolf H. and Huettmann, Gereon and Woermer, Stephan and Koop, Norbert and Beyer, Wolfgang and Jocham, Dieter},
   title = {New balloon catheter system used for PDT in the human urinary bladder: accuracy of light distribution},
   pages = {138-144},
   note = {10.1117/12.308141},
   abstract = {Photodynamic therapy (PDT) may provide a new approach for treatment of patients with superficial transitional carcinoma and carcinoma in situ of the bladder. The light applicator for the bladder wall (Rusch) is constructed as a balloon catheter with two concentric balloons. A new PDT applicator (Rusch) was assessed for the homogeneity and accuracy of irradiation during PDT. In an in-vitro experiment with 17 freshly harvested porcine bladders the fluence rate was measured locally with isotropic detectors. The results were compared to the light fluence detected by the PDT applicator. The increase of the fluence rate (beta) inside the bladders due to back scattering ranged between 5.3 and 7.0 with an average of 6.2. Local variations of the fluence rate in the spherical bladders were also smaller than 15%. Therefore it is concluded, that a homogeneous and accurate irradiation during PDT is possible. Blood between the outer balloon and the bladder wall reduces the local fluence rate strongly and should to be avoided. Also larger air bubbles in the applicator can lead to an inhomogeneous light distribution. In regular application the presented new catheter system provides accurate and easy light dosimetry during PDT of the bladder. Attention had to be paid to a continuous flushing of the space between balloon and bladder wall in order to prevent the accumulation of urine and blood. To avoid a malfunction of the system and large errors in light dosimetry and application, it is advisable to monitor the measured light dosage and the shape of the balloon using ultrasonography during PDT.},
   year = {1998}
}