Publications
2013
H.
Sudkamp,
H.
Lee,
G.
Hüttmann, and
A.
Kellerbee,
An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array, in Studierendentagung , Universität zu Lübeck, 2013.
An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array, in Studierendentagung , Universität zu Lübeck, 2013.
D.
Hillmann,
G.
Franke,
L.
Hinkel,
T.
Bonin,
P.
Koch, and
G.
Hüttmann,
Off-axis full-field swept-source optical coherence tomography using holographic refocusing, pp. 857104-857104, 2013.
Off-axis full-field swept-source optical coherence tomography using holographic refocusing, pp. 857104-857104, 2013.
DOI: | 10.1117/12.2006436 |
File: | 12.2006436 |
Bibtex: | ![]() @article{Hillmann2013, author = {Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon}, title = {Off-axis full-field swept-source optical coherence tomography using holographic refocusing}, pages = {857104-857104}, note = {10.1117/12.2006436}, abstract = {We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.}, DOI = {10.1117/12.2006436}, url = {http://dx.doi.org/10.1117/12.2006436}, year = {2013}, type = {Journal Article} } |
F.
Fleischhauer,
H.
Schulz-Hildebrandt,
T.
Bonin, and
G.
Hüttmann,
Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination, in Studierendentagung , Universität zu Lübeck, 2013.
Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination, in Studierendentagung , Universität zu Lübeck, 2013.
File: | |
Bibtex: | ![]() @inproceedings{Fleischhauer2013, author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon}, title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination}, booktitle = {Studierendentagung}, publisher = {Universität zu Lübeck}, type = {Conference Proceedings}, url = { https://pdfs.semanticscholar.org/a581/a18366acff021e12dcc090b40890ea70dcb8.pdf}, year = { 2013} } |
G.
Franke,
D.
Hillmann,
C.
Luhrs,
P.
Koch,
J.
Wollenzin, and
G.
Hüttmann,
Towards microscopic resolution in holoscopy, pp. 85711O-85711O, 2013.
Towards microscopic resolution in holoscopy, pp. 85711O-85711O, 2013.
DOI: | 10.1117/12.2006806 |
File: | 12.2006806 |
Bibtex: | ![]() @article{Franke2013, author = {Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon}, title = {Towards microscopic resolution in holoscopy}, pages = {85711O-85711O}, note = {10.1117/12.2006806}, abstract = {Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.}, DOI = {10.1117/12.2006806}, url = {http://dx.doi.org/10.1117/12.2006806}, year = {2013}, type = {Journal Article} } |
2009
G.
Hüttmann,
C.
Yao,
X.
Qu,
Z.
Zhang, and
R.
Rahmanzadeh,
Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection, J Biomed Opt , vol. 14, pp. 054034, 2009.
Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection, J Biomed Opt , vol. 14, pp. 054034, 2009.
DOI: | 10.1117/1.3253320 |
Bibtex: | ![]() @article{Yao, author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.}, title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection}, journal = {J Biomed Opt}, volume = {14}, pages = {054034}, note = {Journal article}, year = {2009} } |
2008
X.
Qu,
J.
Wang,
Z.
Zhang,
N.
Koop,
R.
Rahmanzadeh, and
G.
Hüttmann,
Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes, vol. 13, no. 3, pp. 031217, 2008.
Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes, vol. 13, no. 3, pp. 031217, 2008.
DOI: | 10.1117/1.2942373 |
ISBN: | 1083-3668 (Print) 1083-3668 (Linking) |
Bibtex: | ![]() @misc{Qu, author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.}, title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes}, volume = {13}, number = {3}, pages = {031217}, note = {Using Smart Source Parsing May-Jun}, abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.}, ISBN = {1083-3668 (Print) 1083-3668 (Linking)}, year = {2008} } |
C.
Yao,
Z.
Zhang,
R.
Rahmanzadeh, and
G.
Hüttmann,
Laser-based gene transfection and gene therapy, IEEE Trans Nanobioscience , vol. 7, no. 2, pp. 111-9, 2008.
Laser-based gene transfection and gene therapy, IEEE Trans Nanobioscience , vol. 7, no. 2, pp. 111-9, 2008.
DOI: | 10.1109/TNB.2008.2000742 |
Bibtex: | ![]() @article{Yao, author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.}, title = {Laser-based gene transfection and gene therapy}, journal = {IEEE Trans Nanobioscience}, volume = {7}, number = {2}, pages = {111-9}, note = {Yao, C P Zhang, Z X Rahmanzadeh, R Huettmann, G Research Support, Non-U.S. Gov't Review United States IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.}, abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.}, keywords = {Cell Membrane/ radiation effects DNA/ administration & dosage/ pharmacokinetics Gene Therapy/ methods Lasers Transfection/ methods}, year = {2008} } |
2007
R.
Rahmanzadeh,
G.
Hüttmann,
J.
Gerdes, and
T.
Scholzen,
Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis, Cell Prolif , vol. 40, no. 3, pp. 422-30, 2007.
Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis, Cell Prolif , vol. 40, no. 3, pp. 422-30, 2007.
DOI: | 10.1111/j.1365-2184.2007.00433.x |
Bibtex: | ![]() @article{Rahmanzadeh, author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.}, title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis}, journal = {Cell Prolif}, volume = {40}, number = {3}, pages = {422-30}, note = {Rahmanzadeh, R Huttmann, G Gerdes, J Scholzen, T England Cell Prolif. 2007 Jun;40(3):422-30.}, abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.}, keywords = {Antibodies, Antinuclear Antibodies, Monoclonal Cell Division/physiology Cell Nucleolus/physiology Fluorescein-5-isothiocyanate Fluorescent Dyes HeLa Cells Humans Ki-67 Antigen/*genetics/*metabolism Photochemistry RNA Polymerase I/metabolism RNA, Ribosomal/*biosynthesis}, year = {2007} } |
2005
C.
Yao,
R.
Rahmanzadeh,
E.
Endl,
Z.
Zhang,
J.
Gerdes, and
G.
Hüttmann,
Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles, J Biomed Opt , vol. 10, no. 6, pp. 064012, 2005.
Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles, J Biomed Opt , vol. 10, no. 6, pp. 064012, 2005.
DOI: | http://dx.doi.org/10.1117/1.2137321 |
Bibtex: | ![]() @article{Yao, author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.}, title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles}, journal = {J Biomed Opt}, volume = {10}, number = {6}, pages = {064012}, note = {Yao, Cuiping Rahmanzadeh, Ramtin Endl, Elmar Zhang, Zhenxi Gerdes, Johannes Huttmann, Gereon Research Support, Non-U.S. Gov't United States J Biomed Opt. 2005 Nov-Dec;10(6):064012.}, abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.}, keywords = {Biopolymers/pharmacokinetics Cell Line, Tumor Cell Membrane Permeability/ physiology/ radiation effects Drug Delivery Systems/ methods Fluoresceins/ pharmacokinetics Humans Lasers Lymphoma/ metabolism Nanostructures}, year = {2005} } |
2004
P.
Koch,
G.
Huettmann,
H.
Schleiermacher,
J.
Eicholz, and
E.
Koch,
Linear OCT system with down conversion of the fringe pattern, Valery, V. Tuchin and Joseph, A. Izatt and James, G. Fujimoto, Eds. SPIE, 2004. pp. 260-267.
Linear OCT system with down conversion of the fringe pattern, Valery, V. Tuchin and Joseph, A. Izatt and James, G. Fujimoto, Eds. SPIE, 2004. pp. 260-267.
File: | 12.531323 |
Bibtex: | ![]() @inproceedings{Koch-2004, author = {Koch, Peter and Huettmann, Gereon and Schleiermacher, Hansfrieder and Eicholz, Joerg and Koch, Edmund}, title = {Linear OCT system with down conversion of the fringe pattern}, editor = {Valery, V. Tuchin and Joseph, A. Izatt and James, G. Fujimoto}, publisher = {SPIE}, volume = {5316}, pages = {260-267}, Year = { 2004}, URL = { https://doi.org/10.1117/12.531323} } |
2003
G.
Huettmann,
B.
Radt, and
J.
Serbin,
Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses, Rudolf, W. Steiner, Eds. SPIE, 2003. pp. 88-95.
Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses, Rudolf, W. Steiner, Eds. SPIE, 2003. pp. 88-95.
File: | ECBO.2003.5142_88 |
Bibtex: | ![]() @inproceedings{Hüttmann2003, author = {Huettmann, Gereon and Radt, Benno and Serbin, Jesper and Birngruber, Reginald}, title = {Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses}, editor = {Rudolf, W. Steiner}, publisher = {SPIE}, volume = {5142}, pages = {88-95}, URL = { https://doi.org/10.1364/ECBO.2003.5142_88}, year = { 2003} } |
2002
G.
Schuele,
G.
Huettmann, and
R.
Brinkmann,
Noninvasive temperature measurements during laser irradiation of the retina with optoacoustic techniques, Fabrice, Manns and Per, G. Soederberg and Arthur, Ho, Eds. Proc. SPIE, 2002. pp. 64-71.
Noninvasive temperature measurements during laser irradiation of the retina with optoacoustic techniques, Fabrice, Manns and Per, G. Soederberg and Arthur, Ho, Eds. Proc. SPIE, 2002. pp. 64-71.
File: | 12.470601 |
Bibtex: | ![]() @inproceedings{Schuele-2002, author = {Schuele, Georg and Huettmann, Gereon and Brinkmann, Ralf}, title = {Noninvasive temperature measurements during laser irradiation of the retina with optoacoustic techniques}, editor = {Fabrice, Manns and Per, G. Soederberg and Arthur, Ho}, publisher = {Proc. SPIE}, volume = {4611}, pages = {64-71}, year = { 2002}, url = { https://doi.org/10.1117/12.470601} } |
2001
B.
Lange,
B.
Radt, and
G.
Huettmann,
Cr,Tm,Ho: YAG laser amplifier, Richard, Scheps, Eds. SPIE, 2001. pp. 169-174.
Cr,Tm,Ho: YAG laser amplifier, Richard, Scheps, Eds. SPIE, 2001. pp. 169-174.
File: | 12.424616 |
Bibtex: | ![]() @inproceedings{Lange2001, author = {Lange, Bjoern I. and Radt, Benno and Huettmann, Gereon}, title = {Cr,Tm,Ho: YAG laser amplifier}, editor = {Richard, Scheps}, publisher = {SPIE}, volume = {4267}, pages = {169-174}, URL = {https://doi.org/10.1117/12.424616}, Year = { 2001} } |
B.
Radt,
J.
Serbin,
B.
Lange,
R.
Birngruber, and
G.
Huettmann,
Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses, Reginald, Birngruber and Hubert van den, Bergh, Eds. SPIE, 2001. pp. 16-24.
Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses, Reginald, Birngruber and Hubert van den, Bergh, Eds. SPIE, 2001. pp. 16-24.
File: | 12.446518 |
Bibtex: | ![]() @inproceedings{Radt-2001, author = {Radt, Benno and Serbin, Jesper and Lange, Bjoern I. and Birngruber, Reginald and Huettmann, Gereon}, title = {Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses}, editor = {Reginald, Birngruber and Hubert van den, Bergh}, publisher = {SPIE}, volume = {4433}, pages = {16-24}, year = { 2001}, URL = { https://doi.org/10.1117/12.446518} } |
G.
Hüttmann,
J.
Serbin,
B.
Radt,
B.
Lange, and
R.
Birngruber,
Model system for investigating laser-induced subcellular microeffects., Proc SPIE , vol. 4257, pp. 398-409, 2001.
Model system for investigating laser-induced subcellular microeffects., Proc SPIE , vol. 4257, pp. 398-409, 2001.
2000
R.
Brinkmann,
G.
Hüttmann,
J.
Rögener,
J.
Roider,
R.
Birngruber, and
C.
Lin,
Corneal endothelial cell damage after experimental diode laser thermal keratoplasty, Lasers Surg Med , vol. 27(5), pp. 451-64, 2000.
Corneal endothelial cell damage after experimental diode laser thermal keratoplasty, Lasers Surg Med , vol. 27(5), pp. 451-64, 2000.
File: | 227934019_Origin_of_retinal_pigment_epithelium_cell_damage_by_pulsed_laser_irradiance_in_the_nanosecond_to_microsecond_time_regimen |
Bibtex: | ![]() @article{Brinkmann2000-1, author = {Brinkmann, R. and Huttmann, G. and Rogener, J. and Roider, J. and Birngruber, R. and Lin, C. P.}, title = {Origin of retinal pigment epithelium cell damage by pulsed laser irradiance in the nanosecond to microsecond time regimen}, journal = {Lasers Surg Med}, volume = {27(5)}, Year = { 2000}, url = { https://www.researchgate.net/publication/227934019_Origin_of_retinal_pigment_epithelium_cell_damage_by_pulsed_laser_irradiance_in_the_nanosecond_to_microsecond_time_regimen}, pages = {451-64}, note = {0196-8092 (Print) In Vitro Journal Article Research Support, Non-U.S. Gov't}, abstract = {BACKGROUND AND OBJECTIVE: Selective photodamage of the retinal pigment epithelium (RPE) is a new technique to treat a variety of retinal diseases without causing adverse effects to surrounding tissues such as the neural retina including the photoreceptors and the choroid. In this study, the mechanism of cell damage after laser irradiation was investigated. STUDY DESIGN/MATERIALS AND METHODS: Single porcine RPE-melanosomes and RPE cells were irradiated with a Nd:YLF laser (wavelength lambda = 527 nm, adjustable pulse duration tau = 250 nsec-3 microsec) and a Nd:YAG laser (lambda = 532 nm, tau = 8 nsec). Fast flash photography was applied to observe vaporization at melanosomes in suspension. A fluorescence viability assay was used to probe the cells vitality. RESULTS: The threshold radiant exposures for vaporization around individual melanosomes and for ED50 cell damage are similar at 8-nsec pulse duration. Both thresholds increase with pulse duration; however, the ED50 cell damage radiant exposure is 40% lower at 3 microsec. Temperature calculations to model the onset of vaporization around the melanosomes are in good agreement with the experimental results when assuming a surface temperature of 150 degrees C to initiate vaporization and a homogeneous melanosome absorption coefficient of 8,000 cm(-1). Increasing the number of pulses delivered to RPE cells at a repetition rate of 500 Hz, the ED50 value } } |
G.
Hüttmann,
B.
Radt, and
R.
Birngruber,
Laserinduzierte Mikro- und Nanoeffekte - Von der selektiven Thermolyse zu molekularen Nanoeffekten, LaserOpto , vol. 32, pp. 47-55, 2000.
Laserinduzierte Mikro- und Nanoeffekte - Von der selektiven Thermolyse zu molekularen Nanoeffekten, LaserOpto , vol. 32, pp. 47-55, 2000.
G.
Hüttmann, and
R.
Birngruber,
Laserinduzierte thermische Gewebseffekte mit mikroskopischer und makromolekularer Präzision, Z Med Phys , vol. 10, pp. 169-174, 2000.
Laserinduzierte thermische Gewebseffekte mit mikroskopischer und makromolekularer Präzision, Z Med Phys , vol. 10, pp. 169-174, 2000.
G.
Schule,
G.
Hüttmann,
J.
Roider,
C.
Wirbelauer,
R.
Birngruber, and
R.
Brinkmann,
Optoacoustic measurements during µs-irradiation of the retinal pigment epithelium, Proc. SPIE , vol. 3914A, 2000.
Optoacoustic measurements during µs-irradiation of the retinal pigment epithelium, Proc. SPIE , vol. 3914A, 2000.
1999
G.
Hüttmann, and
R.
Birngruber,
On the possibility of high-precision photothermal microeffects and the measurement of fast thermal denaturation of proteins, Ieee Journal of Selected Topics in Quantum Electronics , vol. 5, no. 4, pp. 954-962, 1999.
On the possibility of high-precision photothermal microeffects and the measurement of fast thermal denaturation of proteins, Ieee Journal of Selected Topics in Quantum Electronics , vol. 5, no. 4, pp. 954-962, 1999.
DOI: | Doi 10.1109/2944.796317 |
File: | WOS:000083257800013 |
Bibtex: | ![]() @article{Hüttmann1999, author = {Huttmann, G. and Birngruber, R.}, title = {On the possibility of high-precision photothermal microeffects and the measurement of fast thermal denaturation of proteins}, journal = {Ieee Journal of Selected Topics in Quantum Electronics}, volume = {5}, number = {4}, pages = {954-962}, note = {248CM Times Cited:77 Cited References Count:43}, abstract = {The precision of laser-induced effects is often limited by thermal and thermomechanical collateral damage. Adjusting the pulsewidth of the laser to the size of the absorbing structure can at least avoid thermal side effects and facilitates a selective treatment of vessels or pigmented cells. Further extending the precision of thermal effects below cellular dimensions by using nanometer sized particles could open up new fields of applications for lasers in medicine and biology. Calculations show that under irradiation with nano- or picosecond laser pulses gold particles of submicrometer size can easily be heated by several hundred K, High temperatures have to be used for subcellular thermal effects, because heat confinement to such small structures requires the thermal damage to occur in extremely short times. Estimating the denaturation temperature by extrapolating the Arrhenius equation from a time range of minutes and seconds into a time range of nano- and picoseconds leads to temperatures beta;een 370 K-470 K, There is evidence that in aqueous media, due to the surface tension, these temperatures can be generated at the surface of nanometer sized particles without vaporization of the surrounding water, In order to show whether or not an extrapolation of the damage rates over six to nine orders of magnitude gives correct data, a temperature-jump experiment was designed and tested which allows to measure denaturation rates of proteins in the millisecond time range. Denaturation of chymotrypsin was observed within 300 mu s at temperatures below 380 K, The rate constants for the unfolding of chymotrypsin followed the Arrhenius equation up to rates of 3000 s(-1).}, keywords = {laser medicine microeffects protein denaturation thermal effects assisted laser inactivation}, ISSN = {1077-260X}, DOI = {Doi 10.1109/2944.796317}, url = {<Go to ISI>://WOS:000083257800013}, year = {1999}, type = {Journal Article} } |
G.
Hüttmann, and
R.
Birngruber,
On the Possibility of High-Precision Photothermal Microeffects and the Measurement of Fast thermal Denaturation of Proteins., IEEE J Quant Electr , vol. 5, no. 4, pp. 954-962, 1999.
On the Possibility of High-Precision Photothermal Microeffects and the Measurement of Fast thermal Denaturation of Proteins., IEEE J Quant Electr , vol. 5, no. 4, pp. 954-962, 1999.
R.
Brinkmann,
J.
Rögener,
C.
Lin,
J.
Roider,
R.
Birngruber, and
G.
Hüttmann,
Selective RPE-Photodestruction: Mechanism of Cell Damage by pulsed laser irradiance in the ns to µs time regime, Proc. SPIE , vol. 3601, pp. 59-65, 1999.
Selective RPE-Photodestruction: Mechanism of Cell Damage by pulsed laser irradiance in the ns to µs time regime, Proc. SPIE , vol. 3601, pp. 59-65, 1999.
1998
G.
Hüttmann, and
R.
Birngruber,
Dynamics of Thermal Microeffects: Rate Constants of Thermal Denaturation Measured by a Temperature-Jump Experiment, OSA TOPS , vol. 22, pp. 300-305, 1998.
Dynamics of Thermal Microeffects: Rate Constants of Thermal Denaturation Measured by a Temperature-Jump Experiment, OSA TOPS , vol. 22, pp. 300-305, 1998.
R.
Eichenauer,
G.
Huettmann,
S.
Woermer,
N.
Koop,
W.
Beyer, and
D.
Jocham,
New balloon catheter system used for PDT in the human urinary bladder: accuracy of light distribution, pp. 138-144, 1998.
New balloon catheter system used for PDT in the human urinary bladder: accuracy of light distribution, pp. 138-144, 1998.
1997
C.
Hendrich,
G.
Hüttmann,
C.
Lehnert,
H.
Diddens, and
W.
Siebert,
Photodynamic laser therapy for rheumatoid arthritis: Cell culture studies and animal experiments., Knee Surg Sports Traumatol Arthroscopy , vol. 5, pp. 58-63, 1997.
Photodynamic laser therapy for rheumatoid arthritis: Cell culture studies and animal experiments., Knee Surg Sports Traumatol Arthroscopy , vol. 5, pp. 58-63, 1997.
1996
M.
Novo,
G.
Hüttmann, and
H.
Diddens,
Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumours, J Photoch Photobio , vol. 34, pp. 143-148, 1996.
Chemical instability of 5-aminolevulinic acid used in the fluorescence diagnosis of bladder tumours, J Photoch Photobio , vol. 34, pp. 143-148, 1996.
G.
Hüttmann,
W.
Achtelik,
M.
Löning,
K.
Sommer, and
H.
Diddens,
Fluorescence microscopy studies on ALA sensitized tissues, in SPIE Proc. , 1996. pp. 24-31.
Fluorescence microscopy studies on ALA sensitized tissues, in SPIE Proc. , 1996. pp. 24-31.
File: | 12.260815 |
Bibtex: | ![]() @inproceedings{Hüttmann, author = {Hüttmann, G. and Achtelik, W. and Löning, M. and Sommer, K. and Diddens, H.}, title = {Fluorescence microscopy studies on ALA sensitized tissues}, booktitle = {SPIE Proc.}, volume = {2926}, pages = {24-31}, year = { 1996}, URL = { https://doi.org/10.1117/12.260815} } |
G.
Hüttmann,
C.
Hendrich,
R.
Birngruber,
C.
Lehnert,
J.
Seara,
W.
Siebert, and
H.
Diddens,
Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints . Mechanisms and Techniques in Photodynamic Therapy V,, in Proc. SPIE , 1996. pp. 238-242.
Protoporphyrin IX distribution after intra-articular and systemic application of 5-aminolevulinic acid in healthy and arthritic joints . Mechanisms and Techniques in Photodynamic Therapy V,, in Proc. SPIE , 1996. pp. 238-242.
1994
Y.
Pan,
R.
Engelhardt,
J.
Rosperich,
G.
Hüttmann, and
R.
Birngruber,
Measurement of Optical-Transport-Coefficients of Intralipid in Visible and NIR Range, in Proc. SPIE , 1994. pp. 354-363.
Measurement of Optical-Transport-Coefficients of Intralipid in Visible and NIR Range, in Proc. SPIE , 1994. pp. 354-363.
File: | 12.182954 |
Bibtex: | ![]() @inproceedings{Pan, author = {Pan, Y. and Engelhardt, R. and Rosperich, J. and Hüttmann, G. and Birngruber, R.}, title = {Measurement of Optical-Transport-Coefficients of Intralipid in Visible and NIR Range}, booktitle = {Proc. SPIE}, volume = {2134A}, pages = {354-363}, Year = { 1994}, URL = { https://doi.org/10.1117/12.182954} } |
G.
Hüttmann,
S.
Churio,
S.
Braslavsky, and
R.
Birngruber,
The Possibility of Measuring Thermal Protein Denaturation by an Optoacoustic Method., in Proc. SPIE , 1994. pp. 202-212.
The Possibility of Measuring Thermal Protein Denaturation by an Optoacoustic Method., in Proc. SPIE , 1994. pp. 202-212.
File: | 12.168030 |
Bibtex: | ![]() @inproceedings{Hüttmann, author = {Hüttmann, G. and Churio, S. and Braslavsky, S.E. and Birngruber, R.}, title = {The Possibility of Measuring Thermal Protein Denaturation by an Optoacoustic Method.}, booktitle = {Proc. SPIE}, volume = {2077}, pages = {202-212}, url = { https://doi.org/10.1117/12.168030}, year = { 1994} } |