Claus
Burchard,
Christopher
Kren,
Jan-Erik
Fleger,
Dirk
Theisen-Kunde,
Veit
Danicke,
Hossam S.
Abbas,
Viktoria
Kleyman,
Johann
Roider, and
Ralf
Brinkmann,
Real-Time Temperature-Controlled Retinal Laser Irradiation in Rabbits, Translational Vision Science & Technology , vol. 13, no. 4, pp. 26-26, 04 2024.
Real-Time Temperature-Controlled Retinal Laser Irradiation in Rabbits, Translational Vision Science & Technology , vol. 13, no. 4, pp. 26-26, 04 2024.
DOI: | 10.1167/tvst.13.4.26 |
File: | tvst.13.4.26 |
Bibtex: | @article{10.1167/tvst.13.4.26, author = {von der Burchard, Claus and Kren, Christopher and Fleger, Jan-Erik and Theisen-Kunde, Dirk and Danicke, Veit and Abbas, Hossam S. and Kleyman, Viktoria and Roider, Johann and Brinkmann, Ralf}, title = "{Real-Time Temperature-Controlled Retinal Laser Irradiation in Rabbits}", journal = {Translational Vision Science & Technology}, volume = {13}, number = {4}, pages = {26-26}, year = {2024}, month = {04}, abstract = "{ Subdamaging thermal retinal laser therapy has the potential to induce regenerative stimuli in retinal diseases, but validated dosimetry is missing. Real-time optoacoustic temperature determination and control could close this gap. This study investigates a first in vivo application. Two iterations of a control module that were optically coupled in between a continuous-wave commercial laser source and a commercial slit lamp were evaluated on chinchilla rabbits. The module allows extraction of the temperature rise in real time and can control the power of the therapy laser such that a predefined temperature rise at the retina is quickly achieved and held constant. Irradiations with aim temperatures from 45°C to 69°C were performed on a diameter of 200 µm and a heating time of 100 ms. We analyzed 424 temperature-guided irradiations in nine eyes of five rabbits. The mean difference between the measured and aim temperature was −0.04°C ± 0.98°C. The following ED50 values for visibility thresholds could be determined: 58.6°C for funduscopic visibility, 57.7°C for fluorescein angiography, and 57.0°C for OCT. In all measurements, the correlation of tissue effect was higher to the temperature than to the average heating laser power used. The system was able to reliably perform temperature-guided irradiations, which allowed for better tissue effect control than simple power control. This approach could enhance the accuracy, safety, and reproducibility of thermal stimulating laser therapy. This study is a bridge between preclinical ex vivo experiments and a pilot clinical study. }", issn = {2164-2591}, doi = {10.1167/tvst.13.4.26}, url = {https://doi.org/10.1167/tvst.13.4.26}, eprint = {https://arvojournals.org/arvo/content\_public/journal/tvst/938671/i2164-2591-13-4-26\_1713518675.19154.pdf}, } |
Jessica
Kren,
Patrick
Kuppler,
Steffen
Buschschlüter,
Nicolas
Detrez,
Sazgar
Burhan,
Robert
Huber,
Ralf
Brinkmann, and
Matteo Mario
Bonsanto,
Mechanical characteristics of glioblastoma and peritumoral tumor-free human brain tissue, Acta Neurochirurgica , vol. 166, no. 1, pp. 102, 02 2024.
Mechanical characteristics of glioblastoma and peritumoral tumor-free human brain tissue, Acta Neurochirurgica , vol. 166, no. 1, pp. 102, 02 2024.
DOI: | 10.1007/s00701-024-06009-x |
Bibtex: | @article{RN5472, author = {Kren, Jessica;Skambath, Isabelle;Kuppler, Patrick;Buschschlüter, Steffen;Detrez, Nicolas;Burhan, Sazgar;Huber, Robert;Brinkmann, Ralf and Bonsanto, Matteo Mario}, title = {Mechanical characteristics of glioblastoma and peritumoral tumor-free human brain tissue}, journal = {Acta Neurochirurgica}, volume = {166}, number = {1}, pages = {102}, ISSN = {0942-0940}, DOI = {10.1007/s00701-024-06009-x}, url = {https://doi.org/10.1007/s00701-024-06009-x}, year = {2024}, type = {Journal Article} } |
Svenja
Sonntag,
Britta
Klein,
Ralf
Brinkmann,
Salvatore
Grisanti, and
Yoko
Miura,
Fluorescence Lifetime Imaging Ophthalmoscopy of Mouse Models of Age-related Macular Degeneration, Translational Vision Science & Technology , vol. 13, pp. 24-24, 01 2024.
Fluorescence Lifetime Imaging Ophthalmoscopy of Mouse Models of Age-related Macular Degeneration, Translational Vision Science & Technology , vol. 13, pp. 24-24, 01 2024.
DOI: | 10.1167/tvst.13.1.24 |
Weblink: | https://doi.org/10.1167/tvst.13.1.24 |
Bibtex: | @article{10.1167/tvst.13.1.24, author = {Sonntag, Svenja Rebecca and Klein, Britta and Brinkmann, Ralf and Grisanti, Salvatore and Miura, Yoko}, title = "{Fluorescence Lifetime Imaging Ophthalmoscopy of Mouse Models of Age-related Macular Degeneration}", journal = {Translational Vision Science & Technology}, volume = {13}, number = {1}, pages = {24-24}, year = {2024}, month = {01}, abstract = "{ To investigate fluorescence lifetime of mouse models of age-related macular degeneration (AMD) by fluorescence lifetime imaging ophthalmoscopy (FLIO). Two AMD mouse models, apolipoprotein E knockout (ApoE−/−) mice and NF-E2-related factor-2 knockout (Nrf2−/−) mice, and their wild-type mice underwent monthly ophthalmic examinations including FLIO from 3 months of age. After euthanasia at the age of 6 or 11 months, blood plasma was collected to determine total antioxidant capacity and eyes were enucleated for Oil red O (ORO) lipid staining of chorioretinal tissue. In FLIO, the mean fluorescence lifetime (τm) of wild type shortened with age in both spectral channels. In short spectral channel, τm shortening was observed in both AMD models as well, but its rate was more pronounced in ApoE−/− mice and significantly different from the other strains as months of age progressed. In contrast, in long spectral channel, both model strains showed completely opposite trends, with τm becoming shorter in ApoE−/− and longer in Nrf2−/− mice than the others. Oil red O staining at Bruch's membrane was significantly stronger in ApoE−/− mice at 11 months than the other strains. Plasma total antioxidant capacity was highest in ApoE−/− mice at both 6 and 11 months. The two AMD mouse models exhibited largely different fundus fluorescence lifetime, which might be related to the different systemic metabolic state. FLIO might be able to indicate different metabolic states of eyes at risk for AMD. This animal study may provide new insights into the relationship between early AMD-associated metabolic changes and FLIO findings. }", issn = {2164-2591}, doi = {10.1167/tvst.13.1.24}, url = {https://doi.org/10.1167/tvst.13.1.24}, eprint = {https://arvojournals.org/arvo/content\_public/journal/tvst/938660/i2164-2591-13-1-24\_1706520239.75643.pdf}, } |
Svenja
Sonntag,
Maximilian
Hamann,
Eric
Seifert,
Salvatore
Grisanti,
Ralf
Brinkmann, and
Yoko
Miura,
Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium, Graefe's Archive for Clinical and Experimental Ophthalmology , 2024.
Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium, Graefe's Archive for Clinical and Experimental Ophthalmology , 2024.
DOI: | 10.1007/s00417-024-06449-2 |
File: | s00417-024-06449-2 |
Bibtex: | @article{RN5480, author = {Sonntag, Svenja Rebecca;Hamann, Maximilian;Seifert, Eric;Grisanti, Salvatore;Brinkmann, Ralf and Miura, Yoko}, title = {Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium}, journal = {Graefe's Archive for Clinical and Experimental Ophthalmology}, ISSN = {1435-702X}, DOI = {10.1007/s00417-024-06449-2}, url = {https://doi.org/10.1007/s00417-024-06449-2}, year = {2024}, type = {Journal Article} } |
Patrick
Kuppler,
Paul
Strenge,
Birgit
Lange,
Sonja
Spahr-Hess,
Wolfgang
Draxinger,
Christian
Hagel,
Dirk
Theisen-Kunde,
Ralf
Brinkmann,
Robert
Huber,
Volker
Tronnier, and
Matteo Mario
Bonsanto,
Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence, Journal of Neurosurgery , pp. 1 - 9, 2024. American Association of Neurological Surgeons.
Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence, Journal of Neurosurgery , pp. 1 - 9, 2024. American Association of Neurological Surgeons.
DOI: | 10.3171/2024.1.JNS231511 |
File: | article-10.3171-2024.1.JNS231511.xml |
Patrick
Kuppler,
Paul
Strenge,
Birgit
Lange,
Sonja
Spahr-Hess,
Wolfgang
Draxinger,
Christian
Hagel,
Dirk
Theisen-Kunde,
Ralf
Brinkmann,
Robert
Huber,
Volker
Tronnier, and
Matteo Mario
Bonsanto,
Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence, Journal of Neurosurgery , pp. 1 - 9, 2024. American Association of Neurological Surgeons.
Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence, Journal of Neurosurgery , pp. 1 - 9, 2024. American Association of Neurological Surgeons.
DOI: | 10.3171/2024.1.JNS231511 |
Bibtex: | @article { Microscopeintegratedopticalcoherencetomographyforinvivohumanbraintumordetectionwithartificialintelligence, author = "Patrick Kuppler and Paul Strenge and Birgit Lange and Sonja Spahr-Hess and Wolfgang Draxinger and Christian Hagel and Dirk Theisen-Kunde and Ralf Brinkmann and Robert Huber and Volker Tronnier and Matteo Mario Bonsanto", title = "Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence", journal = "Journal of Neurosurgery", year = "2024", publisher = "American Association of Neurological Surgeons", doi = "10.3171/2024.1.JNS231511", pages= "1 - 9", url = "https://thejns.org/view/journals/j-neurosurg/aop/article-10.3171-2024.1.JNS231511/article-10.3171-2024.1.JNS231511.xml" } |
Sazgar
Burhan,
Nicolas
Detrez,
Katharina
Rewerts,
Paul
Strenge,
Steffen
Buschschlüter,
Jessica
Kren,
Christian
Hagel,
Matteo Mario
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue, Biomed. Opt. Express , vol. 15, no. 2, pp. 1038--1058, 2024. Optica Publishing Group.
Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue, Biomed. Opt. Express , vol. 15, no. 2, pp. 1038--1058, 2024. Optica Publishing Group.
DOI: | 10.1364/BOE.510020 |
File: | abstract.cfm |
Sazgar
Burhan,
Nicolas
Detrez,
Katharina
Rewerts,
Paul
Strenge,
Steffen
Buschschlüter,
Jessica
Kren,
Christian
Hagel,
Matteo Mario
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue, Biomed. Opt. Express , vol. 15, no. 2, pp. 1038--1058, 2024. Optica Publishing Group.
Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue, Biomed. Opt. Express , vol. 15, no. 2, pp. 1038--1058, 2024. Optica Publishing Group.
DOI: | 10.1364/BOE.510020 |
Bibtex: | @article{Burhan:24, author = {Sazgar Burhan and Nicolas Detrez and Katharina Rewerts and Paul Strenge and Steffen Buschschl\"{u}ter and Jessica Kren and Christian Hagel and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber}, journal = {Biomed. Opt. Express}, keywords = {High speed imaging; Imaging systems; In vivo imaging; Magnetic resonance imaging; Phase noise; Phase shift}, number = {2}, pages = {1038--1058}, publisher = {Optica Publishing Group}, title = {Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue}, volume = {15}, month = {Feb}, year = {2024}, url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-15-2-1038}, doi = {10.1364/BOE.510020}, abstract = {During neuro-oncologic surgery, phase-sensitive optical coherence elastography (OCE) can be valuable for distinguishing between healthy and diseased tissue. However, the phase unwrapping process required to retrieve the original phase signal is a challenging and critical task. To address this issue, we demonstrate a one-dimensional unwrapping algorithm that recovers the phase signal from a 3.2\&\#x2005;MHz OCE system. With a processing time of approximately 0.11 s per frame on the GPU, multiple 2\&\#x03C0; wraps are detected and corrected. By utilizing this approach, exact and reproducible information on tissue deformation can be obtained with pixel accuracy over the entire acquisition time. Measurements of brain tumor-mimicking phantoms and human ex vivo brain tumor samples verified the algorithm\&\#x0027;s reliability. The tissue samples were subjected to a 200\&\#x2005;ms short air pulse. A correlation with histological findings confirmed the algorithm\&\#x0027;s dependability.}, } |
Andrey
Boyko,
Birgit
Lange,
Sebastian
Eckert,
Fedor
Mayorov, and
Ralf
Brinkmann,
Signal Enhancement of a Differential Photoacoustic Cell by Connecting the Microphones via Capillaries, Sensors , vol. 24, no. 7, 2024.
Signal Enhancement of a Differential Photoacoustic Cell by Connecting the Microphones via Capillaries, Sensors , vol. 24, no. 7, 2024.
DOI: | 10.3390/s24072105 |
File: | 2105 |
Bibtex: | @Article{s24072105, AUTHOR = {Boyko, Andrey and Lange, Birgit and Eckert, Sebastian and Mayorov, Fedor and Brinkmann, Ralf}, TITLE = {Signal Enhancement of a Differential Photoacoustic Cell by Connecting the Microphones via Capillaries}, JOURNAL = {Sensors}, VOLUME = {24}, YEAR = {2024}, NUMBER = {7}, ARTICLE-NUMBER = {2105}, URL = {https://www.mdpi.com/1424-8220/24/7/2105}, ISSN = {1424-8220}, ABSTRACT = {Differential photoacoustic spectroscopy (DPAS) cells are usually excited on the first longitudinal ring mode, with a microphone situated in the middle of each of the two resonator tubes. However, it is known from other photoacoustic spectroscopy cell designs that connecting the microphones via a capillary can lead to signal enhancement. By means of finite element method (FEM) simulations, we compared such a photoacoustic spectroscopy (PAS) cell with a capillary to a DPAS cell with a capillary attached to each of the two resonators and showed that the behavior of both systems is qualitatively the same: In both the PAS and the DPAS cell, in-phase and anti-phase oscillations of the coupled system (resonator–capillary) can be excited. In the DPAS cell, capillaries of suitable length also increase the pressure signal at the microphones according to the FEM simulations. For different capillary diameters (1.2 mm/1.7 mm/2.2 mm), the respective optimal capillary length (36–37.5 mm) and signal amplification was determined (94%, 70%, 53%). According to the results of these FEM simulations, a significant increase in sensitivity can, therefore, also be achieved in DPAS cells by expanding them with thin tubes leading to the microphones.}, DOI = {10.3390/s24072105} } |
Paul
Strenge,
Birgit
Lange,
Wolfgang
Draxinger,
Christian
Hagel,
Christin
Grill,
Veit
Danicke,
Dirk
Theisen-Kunde,
Sonja
Spahr-Hess,
Matteo M.
Bonsanto,
Robert
Huber,
Heinz
Handels, and
Ralf
Brinkmann,
Demarcation of brain and tumor tissue with optical coherence tomography using prior neural networks}, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 082023. pp. 126321P.
Demarcation of brain and tumor tissue with optical coherence tomography using prior neural networks}, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 082023. pp. 126321P.
DOI: | 10.1117/12.2670907 |
File: | 12.2670907 |
Bibtex: | @inproceedings{10.1117/12.2670907, author = {Paul Strenge and Birgit Lange and Wolfgang Draxinger and Christian Hagel and Christin Grill and Veit Danicke and Dirk Theisen-Kunde and Sonja Spahr-Hess and Matteo M. Bonsanto and Robert Huber and Heinz Handels and Ralf Brinkmann}, title = {{Demarcation of brain and tumor tissue with optical coherence tomography using prior neural networks}}, volume = {12632}, booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media V}, editor = {Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126321P}, keywords = {Brain Tumor, OCT, Optical Coherence Tomography, Prior Network, Glioblastoma Multiforme, Neural Network, Classification}, year = {2023}, doi = {10.1117/12.2670907}, URL = {https://doi.org/10.1117/12.2670907} } |
Wolfgang
Draxinger,
Dirk
Theisen-Kunde,
Lion
Schuetz,
Nicolas
Detrez,
Paul
Strenge,
Maximilian
Rixius,
Veit
Danicke,
Wolfgang
Wieser,
Jessica
Kren,
Patrick
Kuppler,
Sonja
Spar-Hess,
Matteo M.
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 082023. pp. 126270W.
Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 082023. pp. 126270W.
DOI: | 10.1117/12.2670953 |
Bibtex: | @inproceedings{10.1117/12.2670953, author = {Wolfgang Draxinger and Dirk Theisen-Kunde and Lion Schuetz and Nicolas Detrez and Paul Strenge and Maximilian Rixius and Veit Danicke and Wolfgang Wieser and Jessica Kren and Patrick Kuppler and Sonja Spar-Hess and Matteo Mario Bonsanto M.D. and Ralf Brinkmann and Robert Huber}, title = {{Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation}}, volume = {12627}, booktitle = {Translational Biophotonics: Diagnostics and Therapeutics III}, editor = {Zhiwei Huang and Lothar D. Lilge}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126270W}, abstract = {Microscope integrated realtime 4D MHz-OCT operating at high scanning densities are capable of capturing additional visual contrast resolving depth and tissue. Even within a plain C-scan en-face projection structures are recognizable, that are not visible in a white light camera image. With advanced post processing methods, such as absorbtion coefficient mapping, and morphological classifiers more information is extraced. Presentation to the user in an intuitive way poses practical challenges that go beyond the implementation of a mere overlay display. We present our microscope integrated high speed 4D OCT imaging system, its clinical study use for in-vivo brain tissue imaging, and user feedback on the presentation methods we developed.}, keywords = {optical coherence tomography, neurosurgery, tissue contrast, image fusion, surgical guidance, theranostics}, year = {2023}, doi = {10.1117/12.2670953}, URL = {https://doi.org/10.1117/12.2670953} } |
Sazgar
Burhan,
Nicolas
Detrez,
Madita
Göb,
Matteo Mario
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
Advanced FFT-based contrast approach for MHz optical coherence elastography, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 082023. pp. 1263215.
Advanced FFT-based contrast approach for MHz optical coherence elastography, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 082023. pp. 1263215.
DOI: | 10.1117/12.2670957 |
Bibtex: | @inproceedings{10.1117/12.2670957, author = {Sazgar Burhan and Nicolas Detrez and Madita G{\"o}b and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber}, title = {{Advanced FFT-based contrast approach for MHz optical coherence elastography}}, volume = {12632}, booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media V}, editor = {Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1263215}, abstract = {Optical coherence elastography represents mechanical characteristics of biological tissue in so-called mechanical contrast maps. In addition to the standard intensity image, the contrast map illustrates numerous mechanical tissue features that would otherwise be undetectable. This is of great interest as abnormal physiological changes influence the mechanical behavior of the tissue. We demonstrate an advanced mechanical contrast approach based on the phase signal of our 3.2 MHz optical coherence tomography system. The robustness and performance of this contrast approach is evaluated and discussed based on preliminary results. }, keywords = {Optical Coherence Tomography, OCT, Megahertz OCT, Fourier Domain Mode Locking, Optical Coherence Elastography, OCE, Phase-sensitive OCT, Biomechanics}, year = {2023}, doi = {10.1117/12.2670957}, URL = {https://doi.org/10.1117/12.2670957} } |
Anke
Leichtle,
Zuzana
Penxova,
Thorge
Kempin,
David
Leffers,
Martin
Ahrens,
Peter
König,
Ralf
Brinkmann,
Gereon
Hüttmann,
Karl-Ludwig
Bruchhage, and
Hinnerk
Schulz-Hildebrandt,
Dynamic Microscopic Optical Coherence Tomography as a New Diagnostic Tool for Otitis Media, Photonics , vol. 10, no. 6, 06 2023.
Dynamic Microscopic Optical Coherence Tomography as a New Diagnostic Tool for Otitis Media, Photonics , vol. 10, no. 6, 06 2023.
DOI: | 10.3390/photonics10060685 |
File: | 685 |
Bibtex: | @Article{photonics10060685, AUTHOR = {Leichtle, Anke and Penxova, Zuzana and Kempin, Thorge and Leffers, David and Ahrens, Martin and König, Peter and Brinkmann, Ralf and Hüttmann, Gereon and Bruchhage, Karl-Ludwig and Schulz-Hildebrandt, Hinnerk}, TITLE = {Dynamic Microscopic Optical Coherence Tomography as a New Diagnostic Tool for Otitis Media}, JOURNAL = {Photonics}, VOLUME = {10}, YEAR = {2023}, NUMBER = {6}, ARTICLE-NUMBER = {685}, URL = {https://www.mdpi.com/2304-6732/10/6/685}, ISSN = {2304-6732}, ABSTRACT = {Hypothesis: Otitis media (OM) can be successfully visualized and diagnosed by dynamic microscopic optical coherence tomography (dmOCT). Background: OM is one of the most common infectious diseases and, according to the WHO, one of the leading health problems with high mortality in developing countries. Despite intensive research, the only definitive treatment of therapy-refractory OM for decades has been the surgical removal of inflamed tissue. Thereby, the intra-operative diagnosis is limited to the surgeon’s visual impression. Supportive imaging modalities have been little explored and have not found their way into clinical application. Finding imaging techniques capable of identifying inflamed tissue intraoperatively, therefore, is of significant clinical relevance. Methods: This work investigated a modified version of optical coherence tomography with a microscopic resolution (mOCT) regarding its ability to differentiate between healthy and inflamed tissue. Despite its high resolution, the differentiation of single cells with mOCT is often impossible. A new form of mOCT termed dynamic mOCT (dmOCT) achieves cellular contrast using micro-movements within cells based on their metabolism. It was used in this study to establish correlative measurements with histology. Results: Using dmOCT, images with microscopic resolution were acquired on ex vivo tissue samples of chronic otitis media and cholesteatoma. Imaging with dmOCT allowed the visualization of specific and characteristic cellular and subcellular structures in the cross-sectional images, which can be identified only to a limited extent in native mOCT. Conclusion: We demonstrated for the first time a new marker-free visualization in otitis media based on intracellular motion using dmOCT.}, DOI = {10.3390/photonics10060685} } |
Patrick
Kuppler,
Paul
Strenge,
Birgit
Lange,
Sonja
Spahr-Hess,
Wolfgang
Draxinger,
Christian
Hagel,
Dirk
Theisen-Kunde,
Ralf
Brinkmann,
Robert
Huber,
Volker
Tronnier, and
Matteo Mario
Bonsanto,
The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study, Frontiers in Oncology , vol. 13, 04 2023.
The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study, Frontiers in Oncology , vol. 13, 04 2023.
DOI: | 10.3389/fonc.2023.1151149 |
File: | fonc.2023.1151149 |
Bibtex: | Kuppler P, Strenge P, Lange B, Spahr-Hess S, Draxinger W, Hagel C, Theisen-Kunde D, Brinkmann R, Huber R, Tronnier V and Bonsanto MM (2023) The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study. Front. Oncol. 13:1151149. doi: 10.3389/fonc.2023.1151149 |
Alessa
Hutfilz,
Dirk
Theisen-Kunde,
Matteo M.
Bonsanto, and
Ralf
Brinkmann,
Pulsed thulium laser blood vessel haemostasis as an alternative to bipolar forceps during neurosurgical tumour resection, Lasers in Medical Science , vol. 38, pp. 94, 03 2023.
Pulsed thulium laser blood vessel haemostasis as an alternative to bipolar forceps during neurosurgical tumour resection, Lasers in Medical Science , vol. 38, pp. 94, 03 2023.
DOI: | 10.1007/s10103-023-03747-9 |
Weblink: | https://doi.org/10.1007/s10103-023-03747-9 |
Bibtex: | @article{RN5430, author = {Hutfilz, Alessa;Theisen-Kunde, Dirk;Bonsanto, Matteo Mario and Brinkmann, Ralf}, title = {Pulsed thulium laser blood vessel haemostasis as an alternative to bipolar forceps during neurosurgical tumour resection}, journal = {Lasers in Medical Science}, volume = {38}, number = {1}, pages = {94}, ISSN = {1435-604X}, DOI = {10.1007/s10103-023-03747-9}, url = {https://doi.org/10.1007/s10103-023-03747-9}, year = {2023}, type = {Journal Article} } |
Wolfgang
Draxinger,
Dirk
Theisen-Kunde,
Lion
Schützeck,
Nicolas
Detrez,
Paul
Strenge,
Veit
Danicke,
Jessica
Kren,
Patrick
Kuppler,
Sonja
Spahr-Hess,
Matteo Mario
Bonsanto,
Ralf
Brinkmann, and
Robert
Huber,
High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time, in High-Speed Biomedical Imaging and Spectroscopy VIII , Kevin K. Tsia and Keisuke Goda, Eds. SPIE, 032023. pp. 123900D.
High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time, in High-Speed Biomedical Imaging and Spectroscopy VIII , Kevin K. Tsia and Keisuke Goda, Eds. SPIE, 032023. pp. 123900D.
DOI: | 10.1117/12.2648505 |
Bibtex: | @inproceedings{10.1117/12.2648505, author = {Wolfgang Draxinger and Dirk Theisen-Kunde and Lion Sch{\"u}tzeck and Nicolas Detrez and Paul Strenge and Veit Danicke and Jessica Kren and Patrick Kuppler and Sonja Spahr-Hess and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber}, title = {{High speed 4D in-vivo OCT imaging of the human brain: creating high density datasets for machine learning toward identification of malign tissue in real time}}, volume = {12390}, booktitle = {High-Speed Biomedical Imaging and Spectroscopy VIII}, editor = {Kevin K. Tsia and Keisuke Goda}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {123900D}, abstract = {Neuro-surgery is challenged by the difficulties of determining brain tumor boundaries during excisions. Optical coherence tomography is investigated as an imaging modality for providing a viable contrast channel. Our MHz-OCT technology enables rapid volumetric imaging, suitable for surgical workflows. We present a surgical microscope integrated MHz-OCT imaging system, which is used for the collection of in-vivo images of human brains, with the purpose of being used in machine learning systems that shall be trained to identify and classify tumorous tissue.}, keywords = {optical coherence tomography, brain tumor, neurosurgery, machine learning, contrast augmentation, histology dataset, clinical study, in-vivo imaging}, year = {2023}, doi = {10.1117/12.2648505}, URL = {https://doi.org/10.1117/12.2648505} } |
Manabu
Yamamoto,
Yoko
Miura,
Kumiko
Hirayama,
Akika
Kyo,
Takeya
Kohno,
Dirk
Theisen-Kunde,
Ralf
Brinkmann, and
Shigeru
Honda,
Comparative Treatment Study on Macular Edema Secondary to Branch Retinal Vein Occlusion by Intravitreal Ranibizumab with and without Selective Retina Therapy, Life , vol. 13, no. 3, pp. 769, 03 2023.
Comparative Treatment Study on Macular Edema Secondary to Branch Retinal Vein Occlusion by Intravitreal Ranibizumab with and without Selective Retina Therapy, Life , vol. 13, no. 3, pp. 769, 03 2023.
DOI: | 10.3390/life13030769 |
File: | 769 |
Bibtex: | @article{RN5362, author = {Yamamoto, Manabu;Miura, Yoko;Hirayama, Kumiko;Kyo, Akika;Kohno, Takeya;Theisen-Kunde, Dirk;Brinkmann, Ralf and Honda, Shigeru}, title = {Comparative Treatment Study on Macular Edema Secondary to Branch Retinal Vein Occlusion by Intravitreal Ranibizumab with and without Selective Retina Therapy}, journal = {Life}, volume = {13}, number = {3}, pages = {769}, ISSN = {2075-1729}, DOI = {10.3390/life13030769}, url = {https://www.mdpi.com/2075-1729/13/3/769}, year = {2023}, type = {Journal Article} } |
Sazgar
Burhan,
Nicolas
Detrez,
Katharina
Rewerts,
Madita
Göb,
Christian
Hagel,
Matteo M.
Bonsanto,
Dirk
Theisen-Kunde,
Robert
Huber, and
Ralf
Brinkmann,
Characterization of brain tumor tissue by time-resolved, phase-sensitive optical coherence elastography at 3.2 MHz line rate, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 123680F.
Characterization of brain tumor tissue by time-resolved, phase-sensitive optical coherence elastography at 3.2 MHz line rate, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 123680F.
DOI: | 10.1117/12.2648301 |
Bibtex: | @inproceedings{10.1117/12.2648301, author = {Sazgar Burhan and Nicolas Detrez and Katharina Rewerts and Madita G{\"o}b and Christian Hagel and Matteo Mario Bonsanto and Dirk Theisen-Kunde and Robert Huber and Ralf Brinkmann}, title = {{Characterization of brain tumor tissue by time-resolved, phase-sensitive optical coherence elastography at 3.2 MHz line rate}}, volume = {12368}, booktitle = {Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI}, editor = {Caroline Boudoux and James W. Tunnell}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {123680F}, abstract = {Optical coherence elastography (OCE) offers the possibility of obtaining the mechanical behavior of a tissue. When also using a non-contact mechanical excitation, it mimics palpation without interobserver variability. One of the most frequently used techniques is phase-sensitive OCE. Depending on the system, depth-resolved changes in the sub-µm to nm range can be detected and visualized volumetrically. Such an approach is used in this work to investigate and detect transitions between healthy and tumorous brain tissue as well as inhomogeneities in the tumor itself to assist the operating surgeon during tumor resection in the future. We present time-resolved, phase-sensitive OCE measurements on various ex vivo brain tumor samples using an ultra-fast 3.2 MHz swept-source optical coherence tomography (SS-OCT) system with a frame rate of 2.45 kHz. 4 mm line scans are acquired which, in combination with the high imaging speed, allow monitoring and investigation of the sample's behavior in response to the mechanical load. Therefore, an air-jet system applies a 200 ms short air pulse to the sample, whose non-contact property facilitates the possibility for future in vivo measurements. Since we can temporally resolve the response of the sample over the entire acquisition time, the mechanical properties are evaluated at different time points with depth resolution. This is done by unwrapping the phase data and performing subsequent assessment. Systematic ex vivo brain tumor measurements were conducted and visualized as distribution maps. The study outcomes are supported by histological analyses and examined in detail.}, keywords = { Optical Coherence Tomography, Optical Coherence Elastography, Phase-sensitive OCT, Fourier Domain Mode Locking, Brain Tumor, Phase Unwrapping, Tissue Characterization, Biomechanics}, year = {2023}, doi = {10.1117/12.2648301}, URL = {https://doi.org/10.1117/12.2648301} } |
Paul
Strenge,
Birgit
Lange,
Wolfgang
Draxinger,
Christian
Hagel,
Christin
Grill,
Veit
Danicke,
Dirk
Theisen-Kunde,
Sonja
Spahr-Hess,
Matteo M.
Bonsanto,
Robert
Huber,
Heinz
Handels, and
Ralf
Brinkmann,
Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 1236805.
Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography, in Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI , Caroline Boudoux and James W. Tunnell, Eds. SPIE, 032023. pp. 1236805.
DOI: | 10.1117/12.2649963 |
Bibtex: | @inproceedings{10.1117/12.2649963, author = {Paul Strenge and Birgit Lange and Wolfgang Draxinger and Christian Hagel and Christin Grill and Veit Danicke and Dirk Theisen-Kunde and Sonja Spahr-Hess and Matteo M. Bonsanto and Robert Huber and Heinz Handels and Ralf Brinkmann}, title = {{Dual wavelength analysis and classification of brain tumor tissue with optical coherence tomography}}, volume = {12368}, booktitle = {Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI}, editor = {Caroline Boudoux and James W. Tunnell}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1236805}, abstract = {The ill-defined tumor borders of glioblastoma multiforme pose a major challenge for the surgeon during tumor resection, since the goal of the tumor resection is the complete removal, while saving as much healthy brain tissue as possible. In recent years, optical coherence tomography (OCT) was successfully used to classify white matter from tumor infiltrated white matter by several research groups. Motivated by these results, a dataset was created, which consisted of sets of corresponding ex vivo OCT images, which were acquired by two OCT-systems with different properties (e.g. wavelength and resolution). Each image was annotated with semantic labels. The labels differentiate between white and gray matter and three different stages of tumor infiltration. The data from both systems not only allowed a comparison of the ability of a system to identify the different tissue types present during the tumor resection, but also enable a multimodal tissue analysis evaluating corresponding OCT images of the two systems simultaneously. A convolutional neural network with dirichlet prior was trained, which allowed to capture the uncertainty of a prediction. The approach increased the sensitivity of identifying tumor infiltration from 58 % to 78 % for data with a low prediction uncertainty compared to a previous monomodal approach. }, keywords = {optical coherence tomography, oct, brain, classification, tumor, dual wavelength, glioblastoma multiforme, tissue analysis}, year = {2023}, doi = {10.1117/12.2649963}, URL = {https://doi.org/10.1117/12.2649963} } |
Nicolas
Detrez,
Sazgar
Burhan,
Katharina
Rewerts,
Jessica
Kren,
Christian
Hagel,
Matteo Mario
Bonsanto,
Dirk
Theisen-Kunde,
Robert
Huber, and
Ralf
Brinkmann,
Air-Jet based optical coherence elastography: processing and mechanical interpretation of brain tumor data, in Optical Elastography and Tissue Biomechanics X , Kirill V. Larin and Giuliano Scarcelli and Frédérique Vanholsbeeck, Eds. SPIE, 2023. pp. 1238105.
Air-Jet based optical coherence elastography: processing and mechanical interpretation of brain tumor data, in Optical Elastography and Tissue Biomechanics X , Kirill V. Larin and Giuliano Scarcelli and Frédérique Vanholsbeeck, Eds. SPIE, 2023. pp. 1238105.
DOI: | 10.1117/12.2649835 |
File: | 12.2649835 |
Bibtex: | @inproceedings{10.1117/12.2649835, author = {Nicolas Detrez and Sazgar Burhan and Katharina Rewerts and Jessica Kren and Christian Hagel and Matteo Mario Bonsanto and Dirk Theisen-Kunde and Robert Huber and Ralf Brinkmann}, title = {{Air-Jet based optical coherence elastography: processing and mechanical interpretation of brain tumor data}}, volume = {12381}, booktitle = {Optical Elastography and Tissue Biomechanics X}, editor = {Kirill V. Larin and Giuliano Scarcelli and Fr{\'e}d{\'e}rique Vanholsbeeck}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {1238105}, keywords = {Optical Coherence Elastography, Air-Jet, Air-Puff, biomechanics, viscoelasticity, rheology, brain tissue, brain tumor}, year = {2023}, doi = {10.1117/12.2649835}, URL = {https://doi.org/10.1117/12.2649835} } |
Nicolas
Detrez,
Sazgar
Burhan,
Paul
Strenge,
Jessica
Kren,
Christian
Hagel,
Matteo Mario
Bonsanto,
Dirk
Theisen-Kunde,
Robert
Huber, and
Ralf
Brinkmann,
Air-jet based optical coherence elastography of brain tumor tissue: stiffness evaluation by structural histological analysis, in Emerging Technologies for Cell and Tissue Characterization II , Seemantini K. Nadkarni and Giuliano Scarcelli, Eds. SPIE, 2023. pp. 126290M.
Air-jet based optical coherence elastography of brain tumor tissue: stiffness evaluation by structural histological analysis, in Emerging Technologies for Cell and Tissue Characterization II , Seemantini K. Nadkarni and Giuliano Scarcelli, Eds. SPIE, 2023. pp. 126290M.
DOI: | 10.1117/12.2670944 |
File: | 12.2670944 |
Bibtex: | @inproceedings{10.1117/12.2670944, author = {Nicolas Detrez and Sazgar Burhan and Paul Strenge and Jessica Kren and Christian Hagel and Matteo Mario Bonsanto and Dirk Theisen-Kunde and Robert Huber and Ralf Brinkmann}, title = {{Air-jet based optical coherence elastography of brain tumor tissue: stiffness evaluation by structural histological analysis}}, volume = {12629}, booktitle = {Emerging Technologies for Cell and Tissue Characterization II}, editor = {Seemantini K. Nadkarni and Giuliano Scarcelli}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126290M}, keywords = {Optical Coherence Elastography, Air-Jet, Phase-sensitive OCT, Histology Structure Analysis, Color-Deconvolution, Structural Tensors, Brain tumor, Tissue Characterization}, year = {2023}, doi = {10.1117/12.2670944}, URL = {https://doi.org/10.1117/12.2670944} } |
Paula
Enzian,
Birgit
Lange,
Zuzana
Penxova,
Anke
Leichtle,
Yoko
Miura,
Karl-Ludwig
Bruchhage, and
Ralf
Brinkmann,
Fluorescence lifetime imaging microscopy (FLIM) of human middle ear tissue samples, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 2023. pp. 126271T.
Fluorescence lifetime imaging microscopy (FLIM) of human middle ear tissue samples, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 2023. pp. 126271T.
DOI: | 10.1117/12.2670902 |
File: | 12.2670902 |
Bibtex: | @inproceedings{10.1117/12.2670902, author = {Paula Enzian and Birgit Lange and Zuzana Penxov{\'a} and Anke Leichtle and Yoko Miura and Karl-Ludwig Bruchhage and Ralf Brinkmann}, title = {{Fluorescence lifetime imaging microscopy (FLIM) of human middle ear tissue samples}}, volume = {12627}, booktitle = {Translational Biophotonics: Diagnostics and Therapeutics III}, editor = {Zhiwei Huang and Lothar D. Lilge}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126271T}, keywords = {FLIM, autofluorescence, otitis media, cholesteatoma, middle ear, inflammation}, year = {2023}, doi = {10.1117/12.2670902}, URL = {https://doi.org/10.1117/12.2670902} } |
Wolfgang
Draxinger,
Dirk
Theisen-Kunde,
Lion
Schuetz,
Nicolas
Detrez,
Paul
Strenge,
Maximilian
Rixius,
Veit
Danicke,
Wolfgang
Wieser,
Jessica
Kren,
Patrick
Kuppler,
Sonja
Spar-Hess,
Matteo Mario Bonsanto
M.D.,
Ralf
Brinkmann, and
Robert
Huber,
Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 2023. pp. 126270W.
Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation, in Translational Biophotonics: Diagnostics and Therapeutics III , Zhiwei Huang and Lothar D. Lilge, Eds. SPIE, 2023. pp. 126270W.
DOI: | 10.1117/12.2670953 |
File: | 12.2670953 |
Bibtex: | @inproceedings{10.1117/12.2670953, author = {Wolfgang Draxinger and Dirk Theisen-Kunde and Lion Schuetz and Nicolas Detrez and Paul Strenge and Maximilian Rixius and Veit Danicke and Wolfgang Wieser and Jessica Kren and Patrick Kuppler and Sonja Spar-Hess and Matteo Mario Bonsanto M.D. and Ralf Brinkmann and Robert Huber}, title = {{Microscope integrated realtime high density 4D MHz-OCT in neurosurgery: a depth and tissue resolving visual contrast channel and the challenge of fused presentation}}, volume = {12627}, booktitle = {Translational Biophotonics: Diagnostics and Therapeutics III}, editor = {Zhiwei Huang and Lothar D. Lilge}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126270W}, keywords = {optical coherence tomography, neurosurgery, tissue contrast, image fusion, surgical guidance, theranostics}, year = {2023}, doi = {10.1117/12.2670953}, URL = {https://doi.org/10.1117/12.2670953} } |
Dirk
Theisen-Kunde,
Jessica
Kren,
Alessa
Hutfilz,
Matteo Mario
Bonsanto, and
Ralf
Brinkmann,
Clinical evaluation of thulium laser / ultrasonic aspirator combination instrument during neurosurgical tumour resection, 2023.
Clinical evaluation of thulium laser / ultrasonic aspirator combination instrument during neurosurgical tumour resection, 2023.
Weblink: | https://spie.org/european-conference-on-biomedical-optics/presentation/Clinical-evaluation-of-thulium-laser-ultrasonic-aspirator-combination-instrument-during/12627-34?SSO=1 |
Bibtex: | @inproceedings{RN5454, author = {Theisen-Kunde, D;Kren, J;Hutfilz, A;Bonsanto, M and Brinkmann, R}, title = {Clinical evaluation of thulium laser/ultrasonic aspirator combination instrument during neurosurgical tumour resection}, booktitle = {ECBO}, publisher = {SPIE}, url = {https://spie.org/european-conference-on-biomedical-optics/presentation/Clinical-evaluation-of-thulium-laser-ultrasonic-aspirator-combination-instrument-during/12627-34}, type = {Conference Proceedings} } |
Christin
Grill,
Julie-Jacqueline
Kuhl,
Maximiliane Amelie
Schlenz, and
Ralf
Brinkmann,
Monitoring of fatigue damage in monolithic dental CAD/CAM crowns by optical coherence tomography, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 2023. pp. 126320J.
Monitoring of fatigue damage in monolithic dental CAD/CAM crowns by optical coherence tomography, in Optical Coherence Imaging Techniques and Imaging in Scattering Media V , Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno, Eds. SPIE, 2023. pp. 126320J.
DOI: | 10.1117/12.2670874 |
File: | 12.2670874 |
Bibtex: | @inproceedings{10.1117/12.2670874, author = {Christin Grill and Julie-Jacqueline Kuhl and Maximiliane Amelie Schlenz and Ralf Brinkmann}, title = {{Monitoring of fatigue damage in monolithic dental CAD/CAM crowns by optical coherence tomography}}, volume = {12632}, booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media V}, editor = {Benjamin J. Vakoc and Maciej Wojtkowski and Yoshiaki Yasuno}, organization = {International Society for Optics and Photonics}, publisher = {SPIE}, pages = {126320J}, keywords = {Optical Coherence Tomography, OCT, Monolithic dental crowns, CAD/CAM materials, Microcracks, Non-destructive method, Fatigue damage, Dental materials}, year = {2023}, doi = {10.1117/12.2670874}, URL = {https://doi.org/10.1117/12.2670874} } |