In order to deliver monoclonal antibodies into cells we established different antibody-nanoconjugates. The antibodies are released from endosomes by Photochemical internalization (PCI). For this purpose, cells are previously incubated with the photosensitizer BPD. Irradiation with light at a wavelength of 690 nm activates BPD and leads to the opening of endosomal membranes.

A second irradiation at 490 nm activates FITC and leads to the inactivation of Ki-67 and subsequent cell death. The approach has been tested on cells of different tumors such as ovarian cancer, cervical cancer and head and neck cancers.