Publications

2020

  • Tom Pfeiffer and Madita Göb and Wolfgang Draxinger and Sebastian Karpf and Jan Philip Kolb and Robert Huber: Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging. Biomed. Opt. Express, no. 11, pp. 6799-6811, OSA, Nov, 2020
    BibTeX Link
    @article{Pfeiffer:20,
    author = {Tom Pfeiffer and Madita G\"{o}b and Wolfgang Draxinger and Sebastian Karpf and Jan Philip Kolb and Robert Huber},
    journal = {Biomed. Opt. Express},
    keywords = {AG-Huber_OCT; High speed imaging; Image quality; Optical coherence tomography; Swept lasers; Swept sources; Systems design},
    number = {11},
    pages = {6799--6811},
    publisher = {OSA},
    title = {Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging},
    volume = {11},
    month = {Nov},
    year = {2020},
    doi = {10.1364/BOE.402477},
    abstract = {In order to realize adjustable A-scan rates of fast optical coherence tomography (OCT) systems, we investigate averaging of OCT image data acquired with a MHz-OCT system based on a Fourier Domain Mode Locked (FDML) laser. Increased system sensitivity and image quality can be achieved with the same system at the cost of lower imaging speed. Effectively, the A-scan rate can be reduced in software by a freely selectable factor. We demonstrate a detailed technical layout of the strategies necessary to achieve efficient coherent averaging. Since there are many new challenges specific to coherent averaging in swept source MHz-OCT, we analyze them point by point and describe the appropriate solutions. We prove that coherent averaging is possible at MHz OCT-speed without special interferometer designs or digital phase stabilization. We find, that in our system up to \&\#x223C;100x coherent averaging is possible while achieving a sensitivity increase close to the ideal values. This corresponds to a speed reduction from 3.3 MHz to 33 kHz and a sensitivity gain of 20 dB. We show an imaging comparison between coherent and magnitude averaging of a human finger knuckle joint in vivo with 121\&\#x00A0;dB sensitivity for the coherent case. Further, the benefits of computational downscaling in low sensitivity MHz-OCT systems are analyzed.},
    }
    
  • Strauch, Matthias and Kolb, Jan Philip and Merg, Nadine and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert: Evaluation of two-photon fluorescence microscopy for sectioning-free H&E imaging of different tissues. in 32nd Congress of the ESP and XXXIII International Congress of the IAP, 2020
    BibTeX
    @InProceedings{Strauch2020a,
      author    = {Strauch, Matthias and Kolb, Jan Philip and Merg, Nadine and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert},
      booktitle = {32nd Congress of the ESP and XXXIII International Congress of the IAP},
      title     = {Evaluation of two-photon fluorescence microscopy for sectioning-free {H&E} imaging of different tissues},
      year      = {2020},
      keywords  = {AG-Huber_NL},
    }
  • Strauch, Matthias and Kolb, Jan Philip and Weng, Daniel and Wacker, Melanie and Draxinger, Wolfgang and Merg, Nadine and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert: Two-photon microscopy for sectioning-free virtual H&E imaging. in 104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie, 2020
    BibTeX
    @InProceedings{Strauch2020,
      author    = {Strauch, Matthias and Kolb, Jan Philip and Weng, Daniel and Wacker, Melanie and Draxinger, Wolfgang and Merg, Nadine and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert},
      booktitle = {104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie},
      title     = {Two-photon microscopy for sectioning-free virtual {H&E} imaging},
      year      = {2020},
      keywords  = {AG-Huber_NL},
    }
  • Karpf, S;Riche, C T.;Di Carlo, D;Goel, A;Zeiger, W A.;Suresh, A;Portera-Cailliau, C and Jalali, B: Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates. Nature Communications, no. 11(1), pp. 2062, 2020
    BibTeX Link
    @article{Karpf 2020,
       author = {Karpf, S;Riche, C T.;Di Carlo, D;Goel, A;Zeiger, W A.;Suresh, A;Portera-Cailliau, C and Jalali, B},
       title = {Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates},
       journal = {Nature Communications},
       volume = {11(1)},
      keywords={AG-Huber_NL},
       pages = {2062},
       ISSN = {2041-1723},
      
       url = {https://doi.org/10.1038/s41467-020-15618-w},
       year = {2020},
       type = {Journal Article}
    }
    

2019

  • Karpf, S and Jalali, B: Frequency-doubled FDML-MOPA laser in the visible. Opt Lett 44(24), pp. 5913-5916, 2019
    BibTeX Link
    @karpf2019-2,
       author = {Karpf, S and Jalali, B},
       title = {Frequency-doubled FDML-MOPA laser in the visible},
       journal = {Opt Lett 44(24)},
       keywords = {AG-Huber_NL, AG-Huber_FDML},
       pages = {5913-5916},
       DOI = {10.1364/OL.44.005913},
       
       year = {2019},
       type = {Journal Article}
    }
    
  • Jiang, Y;Karpf, S and Jalali, B: Time-stretch LiDAR as a spectrally scanned time-of-flight ranging camera. Nature Photonics, 2019
    BibTeX Link
    @article{Karpf2019,
       author = {Jiang, Y;Karpf, S and Jalali, B},
       title = {Time-stretch LiDAR as a spectrally scanned time-of-flight ranging camera},
       journal = {Nature Photonics},
       keywords={AG-Huber_NL},
       ISSN = {1749-4893},
       URL = {https://www.researchgate.net/publication/337692338_Time-stretch_LiDAR_as_a_spectrally_scanned_time-of-flight_ranging_camera},
       year = {2019},
       type = {Journal Article}
    }
     
    
    
  • Kolb, Jan Philip and Strauch, Matthias and Weng, Daniel and Hakert, Hubertus and Draxinger, Wolfgang and Merg, Nadine and Brinkmann, Ralf and Birngruber, Reginald and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert: Fiber-Based Sub-Nanosecond Two-Photon Microscopy for Virtual H&E Histology. in European Conference on Biomedical Optics (ECBO), 2019
    BibTeX
    @InProceedings{Kolb2019,
      author    = {Kolb, Jan Philip and Strauch, Matthias and Weng, Daniel and Hakert, Hubertus and Draxinger, Wolfgang and Merg, Nadine and Brinkmann, Ralf and Birngruber, Reginald and Hundt, Jennifer and Karpf, Sebastian and Huber, Robert},
      booktitle = {European Conference on Biomedical Optics (ECBO)},
      title     = {Fiber-Based Sub-Nanosecond Two-Photon Microscopy for Virtual H&E Histology},
      year      = {2019},
      keywords  = {AG-Huber_NL},
    }
  • Strauch, Matthias and Kolb, Jan Philip and Weng, Daniel and Wacker, Melanie and Draxinger, Wolfgang and Karpf, Sebastian and Huber, Robert: Sectioning-Free Virtual H&E Imaging of Tissue Samples with Two-Photon Microscopy. in 31st Congress of the ESP, 2019
    BibTeX
    @InProceedings{Strauch2019,
      author    = {Strauch, Matthias and Kolb, Jan Philip and Weng, Daniel and Wacker, Melanie and Draxinger, Wolfgang and Karpf, Sebastian and Huber, Robert},
      booktitle = {31st Congress of the ESP},
      title     = {Sectioning-Free Virtual H&E Imaging of Tissue Samples with Two-Photon Microscopy},
      year      = {2019},
      keywords  = {AG-Huber_NL},
    }
  • Weng, Daniel and Hakert, Hubertus and Bloemker, Torben and Kolb, Jan Philip and Strauch, Matthias and Eibl, Matthias and Lamminger, Philipp and Karpf, Sebastian and Huber, Robert: Sub-Nanosecond Pulsed Fiber Laser for 532nm Two-Photon Excitation Fluorescence (TPEF) Microscopy of UV Transitions. in 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC), IEEE, 2019
    BibTeX Link
    @InProceedings{Weng2019,
      author    = {Weng, Daniel and Hakert, Hubertus and Bloemker, Torben and Kolb, Jan Philip and Strauch, Matthias and Eibl, Matthias and Lamminger, Philipp and Karpf, Sebastian and Huber, Robert},
      booktitle = {2019 Conference on Lasers and Electro-Optics Europe {\&} European Quantum Electronics Conference ({CLEO}/Europe-{EQEC})},
      title     = {Sub-Nanosecond Pulsed Fiber Laser for 532nm Two-Photon Excitation Fluorescence ({TPEF}) Microscopy of {UV} Transitions},
      year      = {2019},
      publisher = {{IEEE}},
      abstract  = {Summary form only given. Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan. Recently we demonstrated a fully fiber-based laser source built around a directly modulated, ytterbium amplified 1064 nm laser diode with sub-nanosecond pulses for two-photon imaging [2]. The overall system enables to capture high-speed fluorescence lifetime imaging (FLIM) with single pulse excitation. Here, we extend the spectral range of this laser source by frequency doubling it to 532nm to achieve two-photon excited fluorescence microscopy (TPM) in the ultraviolett (UV) range to harness endogenous autofluorescence. In this presentation we explore first TPM results of tryptophan to investigate signal levels and fi delity before transitioning to biological tissues. It has been shown that TPM of endogenous tryptophan can be used to visualize immune system activity in vivo. Our laser source could be a cheap, flexible and fiber-based alternative to the OPO-based Ti:Sa Lasers currently employed. The basic concept of our design is to shift the wavelength of the pulsed fiber-based master oscillator power amplifier (MOPA) by second-harmonic generation (SHG) using phase-matching in a KTP crystal. This generates a coherent output at 532nm at a maximal peak power of 500W. We achieved a maximum conversion efficiency of about 17%. After the SHG module, the 532nm light is coupled into a single-mode fiber and delivered to a home built microscope. A 40x microscope objective is used to excite the sample and epi-collect the fluorescence. The fluorescence is recorded on a UV-enhanced photomultiplier tube (PMT). For a proof of concept measurement, crystalized tryptophan was imaged. Here we show signals of pure tryptophan, with laser parameters of 1MHz repetition rate and 100ps pulse duration. We used spectral bandpass fi lters in order to detect only fluorescence signal, however, from crystalized tryptophan we observed an unexpected short lifetime. We have recently shown that we can shift our laser output from 1064nm to longer wavelengths. By shifting to 1180nm and frequency doubling to 590nm a more efficient fluorescence excitation of tryptophan can be achieved. In the future we aim at in vivo imaging with our setup.},
      doi       = {10.1109/CLEOE-EQEC.2019.8872571},
      keywords  = {AG-Huber_NL;amplifiers;biological tissues;biomedical optical imaging;cellular biophysics;fluorescence;laser applications in medicine;medical image processing;molecular biophysics;optical microscopy;proteins;two-photon processes;ultraviolet spectra;two-photon excitation fluorescence microscopy;UV transitions;excitation wavelengths;fluorescence techniques;live cell imaging;subnanosecond pulsed fiber laser;TPEF microscopy;exogenous fluorophores;autofluorescence;endogenous proteins;fiber-based laser source;laser diode;two-photon imaging;high-speed fluorescence lifetime imaging;FLIM;single pulse excitation;tryptophan;biological tissues;immune system activity;OPO-based Ti:Sa lasers;pulsed fiber-based master oscillator power amplifier;MOPA;second-harmonic generation;SHG;phase-matching;KTP crystal;UV-enhanced photomultiplier tube;laser parameters;spectral bandpass filters;fluorescence excitation;Fluorescence;Laser excitation;Fiber lasers;Microscopy;Laser transitions;In vivo},
    }
  • Karpf, S and Jalali, B: Fourier-domain mode-locked laser combined with a master-oscillator power amplifier architecture. J Opt Lett 44(8), pp. 1952-1955, 2019
    BibTeX Link
    @article{Karpf2019,
       author = {Karpf, S and Jalali, B },
       title = {Fourier-domain mode-locked laser combined with a master-oscillator power amplifier architecture},
       journal = {J Opt Lett} {44(8)},
       URL = {https://doi.org/10.1364/OL.44.001952},
       pages = {1952-1955},
       ISSN = {1539-4794},
       year = {2019},
     keywords = {AG-Huber},
       type = {Journal Article}
    }
    
  • Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R;Popp, J;Limpert, J;Karpf, S and Huber, R: Virtual HE histology by fiber-based picosecond two-photon microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIX Proc. SPIE,10882, pp. 108822F, International Society for Optics and Photonics, 2019
    BibTeX Link Datei
    @inproceedings{Kolb2019/2,
       author = {Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R;Popp, J;Limpert, J;Karpf, S and Huber, R},
       title = {Virtual HE histology by fiber-based picosecond two-photon microscopy},
       booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX} {Proc. SPIE,10882},
       editor = {Ammasi Periasamy; Peter T. C. So; Karsten König},
       publisher = { International Society for Optics and Photonics},
       year = {2019},
       pages = {108822F},
    keywords = {AG-Huber_NL},
       
    doi = {10.1117/12.2507866},
       type = {Conference Proceedings}
    }
    
  • Hakert, Hubertus;Eibl, Matthias;Karpf, Sebastian;Wollenberg, Barbara;Pries, Ralph and Huber, Robert: Label-free imaging of tumorous tissue in the Raman fingerprint region with time-encoded (TICO) stimulated Raman scattering (Conference Presentation). in Multiphoton Microscopy in the Biomedical Sciences XIX, no. 10882, pp. 108821R, International Society for Optics and Photonics,
    BibTeX
    @inproceedings{Hakert2019,
       author = {Hakert, Hubertus;Eibl, Matthias;Karpf, Sebastian;Wollenberg, Barbara;Pries, Ralph and Huber, Robert},
       title = {Label-free imaging of tumorous tissue in the Raman fingerprint region with time-encoded (TICO) stimulated Raman scattering (Conference Presentation)},
       booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX},
       publisher = {International Society for Optics and Photonics},
       volume = {10882},
       pages = {108821R},
       type = {Conference Proceedings}
    }
    

2018

  • Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S: Wavelength agile multi-photon microscopy with a fiber amplified diode laser. Biomedical Optics Express 9(12), pp. 6273-6282, 2018
    BibTeX Link
    @article{Eibl2018,
    author = { Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S},
    title = {Wavelength agile multi-photon microscopy with a fiber amplified diode laser},
    journal = {Biomedical Optics Express} {9(12)},
    keywords = {AG-Huber_NL},
    year = {2018},
    pages = {6273--6282},
    doi = {10.1364/BOE.9.006273},
    }
    

2017

  • Eibl, M; Karpf, S; Hakert, H; Blömker, T; Kolb, J P; Jirauschek, C and Huber, R: Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification. Opt Lett 42(21), pp. 4406-4409, OSA, Nov, 2017
    BibTeX Link
    @article{2017Eibl,
    author = {Eibl, M; Karpf, S; Hakert, H; Bl\"{o}mker, T; Kolb, J P; Jirauschek, C and Huber, R},
    journal = {Opt Lett} {42(21)},
    keywords = {NLI, TPEF, Multiphoton, AG-Huber_NL},
    
    pages = {4406--4409},
    publisher = {OSA},
    title = {Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification},
    
    month = {Nov},
    year = {2017},
    doi = {10.1364/OL.42.004406},
    }
    
  • Eibl, M; Karpf, S; Hakert, H; Weng, D; Pfeiffer, T; Kolb, J P and Huber, R: Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup. in Proc. SPIE 10414, pp. 1041403-1041403-7, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, M; Karpf, S; Hakert, H; Weng, D; Pfeiffer, T; Kolb, J P and Huber, R},
       title = {Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup},
      booktitle = {Proc. SPIE} {10414},
      
      year = {2017},
    keywords = {AG-Huber_NL},
       pages = {1041403-1041403-7},
      
     url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10414/1/Single-pulse-two-photon-fluorescence-lifetime-imaging-SP-FLIM-with/10.1117/12.2286035.short?SSO=1},
       type = {Conference Proceedings}
    }
    
  • Hakert, H; Eibl, M; Karpf, S and Huber, R: Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition. in Proc. SPIE 10414, pp. 1041408-1041408-7, 2017
    BibTeX Link
    @inproceedings{Hakert2017,
       author = {Hakert, H; Eibl, M; Karpf, S and Huber, R},
       title = {Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition},
    booktitle = {Proc. SPIE} {10414},
    Year={2017},
       
       pages = {1041408-1041408-7},
       url = {http://dx.doi.org/10.1117/12.2287947},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    
  • Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber: Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate. Biomedical Optics Express, no. 8, pp. 3132-3142, OSA, Jul, 2017
    BibTeX Link Link
    @article{HU_2017_Eibla_BOE,
    author = {Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber},
    journal = {Biomedical Optics Express},
    number = {7},
    pages = {3132--3142},
    publisher = {OSA},
    title = {Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate},
    volume = {8},
    month = {Jul},
    year = {2017},
    keywords = {AG-Huber_NL},
    url = { https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-8-7-3132&id=367232},
    DOI = { https://doi.org/10.1364/BOE.8.003132}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Shot-Noise Limited Time-Encoded Raman Spectroscopy. Journal of Spectroscopy, no. 2017, pp. 1-6, 2017
    BibTeX Link
    @article{Karpf2017,
       author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
       title = {Shot-Noise Limited Time-Encoded Raman Spectroscopy},
       journal = {Journal of Spectroscopy},
       volume = {2017},
       pages = {1-6},
       url = {https://doi.org/10.1155/2017/9253475},
       year = {2017},
    keywords = {AG-Huber_NL},
       type = {Journal Article}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert: Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging. in Proc. SPIE, no. 10083, pp. 100831C-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert},
       title = {Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging},
    booktitle = {Proc. SPIE },
       volume = {10083},
       pages = {100831C-6},
       year= {2017},
       doi= {10.1117/12.2251965},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert: Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection. in Proc. SPIE, no. 10069, pp. 100691F-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert},
       title = {Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection},
    booktitle = {Proc. SPIE },
       volume = {10069},
       pages = {100691F-6},
       doi= {10.1117/12.2250831},
      year={2017},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    

2016

  • Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert: Two-photon microscopy using fiber-based nanosecond excitation. Biomedical Optics Express, no. 7, pp. 2432-2440, 2016
    BibTeX Link
    @article{Karpf2016,
       author = {Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert},
       title = {Two-photon microscopy using fiber-based nanosecond excitation},
       journal = {Biomedical Optics Express},
       volume = {7},
       number = {7},
       pages = {2432-2440},
       abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
       keywords = {AG-Huber_NL},
       DOI = {10.1364/BOE.7.002432},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Karpf, Sebastian and Eibl, Matthias and Huber, Robert: Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser. in Proc. SPIE, no. 9536, pp. 953616-953616-5, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_c,
    author = {Karpf, Sebastian and Eibl, Matthias and Huber, Robert},
    title = { Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953616-953616-5},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183854}
    }
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Hyperspectral stimulated Raman microscopy with two fiber laser sources. in Proc. SPIE, no. 9536, pp. 953604-953604-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Eibl_a,
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = {Hyperspectral stimulated Raman microscopy with two fiber laser sources },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953604-953604-6},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183822}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers. in Proc. SPIE, no. 9541, pp. 95410F-95410F-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_b,
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = { Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers },
    booktitle = {Proc. SPIE },
    volume = {9541},
    type = {Conference Proceedings},
    pages = {95410F-95410F-6},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183859}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy. Nature Communications, no. 6, pp. 784 1-6;, 2015
    BibTeX Link
    @Article{HU_2015_Karpf_a,
      Title                    = {A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy},
      Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
      Journal                  = {Nature Communications},
      Year                     = {2015},
      Volume = {6},
      pages = {6784 1--6};
    keywords = {AG-Huber_NL},
      Doi                      = {10.1038/ncomms7784}
    }
    

2014

  • Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert: A 4-D OCT Engine with 1 GVoxel/s. Optics and Photonics News, no. 25, pp. 36, OSA, c,, 2014
    BibTeX Link
    @Article{HU_2014_Wieser_b,
      Title                    = {{A 4-D OCT Engine with 1 GVoxel/s}},
      Author                   = {Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Optics and Photonics News},
      Year                     = {2014},
    
      Month                    = dec,
      Number                   = {12},
      Pages                    = {36 },
      Volume                   = {25},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
    
      Publisher                = {OSA},
      Url                      = { http://www.osa-opn.org/home/articles/volume_25/december_2014/extras/a_4-d_oct_engine_with_1_gvoxel_s/#.VcH21Pl5raw}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Time-Encoded Raman: Fiber-based, hyperspectral, broadband stimulated Raman microscopy. ArXiv e-prints, 2014
    BibTeX Link
    @Article{HU_2014_Karpf_a,
      Title                    = {{Time-Encoded Raman: Fiber-based, hyperspectral, broadband stimulated Raman microscopy}},
      Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
      journal = {ArXiv e-prints},
      Year                     = {2014},
      Archiveprefix            = {arXiv},
      Arxivid                  = {1405.4181},
      Eprint                   = {1405.4181},
    keywords = {AG-Huber_NL},
      Url                      = {http://arxiv.org/abs/1405.4181}
    }
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. ATu3P.4 1-3, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Eibl_a,
    abstract = {A fast all fiber based setup for stimulated Raman spectroscopy with a rapidly wavelength swept cw-laser is presented. It enables flexible acquisition of broadband (750 cm-1 to 3150 cm-1) spectra with high resolution (0.5 cm-1).},
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_AT.2014.ATu3P.4},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,stimulated Raman,tunable,AG-Huber_NL},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_AT-2014-ATu3P.4},
    year = { 2014}
    }
  • Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert: High definition live 3D-OCT in vivo: design and evaluation of a 4D OCT engine with 1 GVoxel/s. Biomedical optics express, no. 5, pp. 2963-77, p,, 2014
    BibTeX Link Link
    @Article{HU_2014_Wieser_a,
      Title                    = {{High definition live 3D-OCT in vivo: design and evaluation of a 4D OCT engine with 1 GVoxel/s}},
      Author                   = {Wieser, Wolfgang and Draxinger, Wolfgang and Klein, Thomas and Karpf, Sebastian and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2014},
    
      Month                    = sep,
      Number                   = {9},
      Pages                    = {2963--77},
      Volume                   = {5},
    
      Doi                      = {10.1364/BOE.5.002963},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-9-2963}
    }
  • Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert: Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. 1-2, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Karpf_b,
    abstract = {A hyperspectral stimulated Raman microscopy system using rapidly wavelength swept lasers is presented. Imaging of biological samples with shot noise limited detection is demonstrated with the fiber based setup.},
    author = {Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_SI.2014.SM3P.3},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman microscopy,Scattering,stimulated Raman,tunable, AG-Huber_NL},
    pages = {1--2},
    publisher = {OSA},
    title = {{Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_SI-2014-SM3P.3},
    year = { 2014}
    }

2013

  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: New High Resolution SRS with ultra broadband spectral coverage. in 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference, pp. PD_A_8 1-3, Optical Society of America, Munich, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_a,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    address = {Munich},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference},
    pages = {PD_A_8 1--3},
    publisher = {Optical Society of America},
    doi={10.1109/CLEOE-IQEC.2013.6801995},
    title = {{FDML Raman: New High Resolution SRS with ultra broadband spectral coverage}},
    url = {https://doi.org/10.1109/CLEOE-IQEC.2013.6801995},
    keywords = {AG-Huber_NL},
    year = { 2013}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers. in CLEO: 2013, pp. ATu3P.4 1-3, OSA, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_b,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2013},
    doi = {10.1364/CLEO_SI.2013.CTu2H.5},
    isbn = {978-1-55752-972-5},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,fiber,stimulated Raman,AG-Huber_NL},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_SI-2013-CTu2H.5},
    year = { 2013}
    }

2012

  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert: Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment. in Conference on Lasers and Electro-Optics 2012, pp. JTh3J.2 1-3, OSA, Washington, D.C., 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Wieser_c,
      Title                    = {{Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert},
      Booktitle                = {Conference on Lasers and Electro-Optics 2012},
      Year                     = {2012},
    
      Address                  = {Washington, D.C.},
      Pages                    = {JTh3J.2 1--3},
      Publisher                = {OSA},
    
      Doi                      = {10.1364/CLEO_AT.2012.JTh3J.2},
      ISBN                     = {978-1-55752-943-5},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_AT-2012-JTh3J.2}
    }
  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert: Extended coherence length megahertz FDML and its application for anterior segment imaging. Biomedical optics express, no. 3, pp. 2647-57, t,, 2012
    BibTeX Link Link
    @Article{HU_2012_Wieser_b,
      Title                    = {{Extended coherence length megahertz FDML and its application for anterior segment imaging}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = oct,
      Number                   = {10},
      Pages                    = {2647--57},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002647},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-10-2647}
    }