Dr. rer. nat. Hinnerk Schulz-Hildebrandt

Gruppenleiter


Institut für Biomedizinische Optik
CBBM
Marie-Curie-Straße
23562 Lübeck

Gebäude 66, Raum 20.20

Email:
Phone:
+49 (0)451 3101 3236
Fax:
+49 (0)451 3101 3204




2019

  • Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber,R; Manstein, D and Hüttmann, G: Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography. J Biomed Opt 24(4), pp. 046005 - 1 - 11, 2019
    BibTeX Link
    @article{
    citation_key = { Casper19}
    author = { Casper, M;  Schulz-Hildebrandt, H; Evers, M; Birngruber,R; Manstein, D and Hüttmann, G},
    title = {Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography},
    
    journal = {J Biomed Opt} {24(4)},
    
    pages = {046005 - 1 - 11},
    year = {2019},
    
    URL = {https://doi.org/10.1117/1.JBO.24.4.046005},
    eprint = {}
    }
  • Ourak, M. Smits, J. Esteveny, L. Borghesan, G. Gijbels, A. Schoevaerdts, L. Douven, Y. Scholtes, J. Lankenau, E. Eixmann, T. Schulz-Hildebrandt, H. Hüttmann, G. Kozlovszky, M. Kronreif, G. Willekens, K. Stalmans, P. Faridpooya, K. Cereda, M. Giani, A. Staurenghi, G. Reynaerts, D. Vander Poorten, E. B.: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. International Journal of Computer Assisted Radiology and Surgery, no. 14, pp. 301-309, Feb, 2019
    BibTeX Link Link
    @Article{Ourak2019,
    author="Ourak, M.
    and Smits, J.
    and Esteveny, L.
    and Borghesan, G.
    and Gijbels, A.
    and Schoevaerdts, L.
    and Douven, Y.
    and Scholtes, J.
    and Lankenau, E.
    and Eixmann, T.
    and Schulz-Hildebrandt, H.
    and H{\"u}ttmann, G.
    and Kozlovszky, M.
    and Kronreif, G.
    and Willekens, K.
    and Stalmans, P.
    and Faridpooya, K.
    and Cereda, M.
    and Giani, A.
    and Staurenghi, G.
    and Reynaerts, D.
    and Vander Poorten, E. B.",
    title="Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation",
    journal="International Journal of Computer Assisted Radiology and Surgery",
    year="2019",
    month="Feb",
    day="01",
    volume="14",
    number="2",
    pages="301--309",
    abstract="Retinal vein cannulation is an experimental procedure during which a clot-dissolving drug is injected into an obstructed retinal vein. However, due to the fragility and minute size of retinal veins, such procedure is considered too risky to perform manually. With the aid of surgical robots, key limiting factors such as: unwanted eye rotations, hand tremor and instrument immobilization can be tackled. However, local instrument anatomy distance and force estimation remain unresolved issues. A reliable, real-time local interaction estimation between instrument tip and the retina could be a solution. This paper reports on the development of a combined force and distance sensing cannulation needle, and its experimental validation during in vivo animal trials.",
    issn="1861-6429",
    doi="10.1007/s11548-018-1829-0",
    url="https://doi.org/10.1007/s11548-018-1829-0"
    citation_key = {Ourak2019}
    }

2018

  • Borghesan,G; Ourak,M; Lankenau, E; Hüttmann,G; Schulz-Hildebrandt,H; Willekens,K; Stalmans, K; Reynaerts, D and Vander Poorten,E: Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation. J Med Robot Res 3(2), pp. 184005, 2018
    BibTeX Link Link
    @article{borghesan2018single,
    title = {Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation},
    author = {Borghesan,G; Ourak,M; Lankenau, E; H\"{u}ttmann,G; Schulz-Hildebrandt,H; Willekens,K; Stalmans, K; Reynaerts, D and Vander Poorten,E},
    editor = {World Scientific},
    url = {https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    doi = {10.1142/S2424905X18400056},
    year = {2018},
    date = {2018-02-12},
    journal = {J Med Robot Res} {3(2)},
    
    pages = {184005},
    abstract = {Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures or to inadvertently injure the underlying retina. Damage to the latter is mostly irreparable and might cause permanent loss of vision. Despite the availability over excellent stereo microscopes, wide-angle lenses and powerful light source visualization remains a problem. Especially, the limited depth perception is still perceived as a major bottle-neck whereas efforts have been conducted to integrate sensing capability in today’s state-of-the-art instruments, so far, little effort has been paid to process the obtained sensor data and turns this into a reliable source of information upon which robot assistive guidance schemes could be endowed upon. This paper proposes a number of processing techniques tailored to Optical Coherence Tomography (OCT) measurements. The first results of the proposed algorithms show that it is feasible to extract good and reliable distance estimates from this otherwise rather noisy signal and from a fairly limited dataset. The used data are the so-called A-scans. These are OCT measurements consisting of a single-line image that could be captured by an instrument-mounted fiber through which the OCT signal passes back-and-forth. However, in this work, we perform a pilot study whereby the employed A-scans are extracted from B-scans that are captured by a microscope-mounted OCT scanner, rather than obtained from a probe. The performance of a first embodiment of the algorithm that is based on an Unscented Kalman Filter (UKF) is compared to the performance of a second embodiment that relies on a Particle Filter (PF), focusing on the issues in filter initialization and the tracking quality. Finally, results of UKF and PF executions on a validation dataset are presented. 
    Read More: https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber, R; Manstein, D and Hüttmann, G: Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation). Proc.SPIE 10467, in Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 2018
    BibTeX Link Link
    @conference{Casper2018,
    title = {Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation)},
    author = {Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber, R; Manstein, D and H\"{u}ttmann, G },
    url = {https://doi.org/10.1117/12.2292005},
    doi = {10.1117/12.2292005},
    year = {2018},
    date = {2018-03-14},
    booktitle = {Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018},
    journal = {Proc.SPIE} {10467},
    
    abstract = {The ability to image the physiology of microvasculature with high spatial resolution in three dimensions while investigating structural changes of skin, is essential for understanding the complex processes of skin aging, wound healing and disease development. Further, the quantitative, automatic assessment of these changes enables to analyze large amounts of image data in an abstract but comprehensive manner. 
    However, previous work using OCT with methods of angiography was imaging less scattering, hence more challenging tissue than skin, such as brain and retina tissue. The published methods for capillary segmentation were mostly non-automatic, poorly benchmarked against state-of-the-art methods of computer vision and not applied to investigate medical processes and studies in a comprehensive fashion. 
    Here, segmentation of capillaries in skin is reported and its efficacy is demonstrated in both, a 
    longitudinal mouse study and a preliminary study in humans. By combining state-of-the-art image 
    processing methods in an optimized way, we were able to improve the segmentation results and analyze the impact of each post-processing step. 
    Furthermore, this automatic segmentation enabled us to analyze big amounts of 
    datasets automatically and derive meaningful conclusions for the planning of clinical studies. 
    With this work, optical coherence tomography is combined with methods of computer vision to a diagnostic 
    tool with unique capabilities to characterize vascular diversity and provide extraordinary 
    opportunities for dermatological investigation in both, clinics and research.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Schulz-Hildebrandt, H; Pieper, M; Stehmar,C; Ahrens, M; Idel, C; Wollenberg,B; König,P and Gereon Hüttmann: Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography. Biomedical Optics Express, pp. 636-647, 2018
    BibTeX Link Link
    @article{schulz2018novel,
    title = {Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography},
    author = {Schulz-Hildebrandt, H; Pieper, M; Stehmar,C; Ahrens, M; Idel, C; Wollenberg,B; K\"{o}nig,P and Gereon H\"{u}ttmann},
    editor = {Optic Socie Amer} {9(2)},
    url = {https://www.osapublishing.org/abstract.cfm?URI=boe-9-2-636
    https://www.osapublishing.org/boe/viewmedia.cfm?uri=boe-9-2-636&seq=0},
    doi = {10.1364/BOE.9.000636},
    isbn = {10.1364/BOE.9.000636},
    year = {2018},
    date = {2018-01-16},
    journal = {Biomedical Optics Express},
    
    pages = {636-647},
    abstract = {Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 \textendash 2 μm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests overall a better image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 μm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was determined successfully. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.},
    keywords = {Endoskope, OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Schulz-Hildebrandt, H; Pfeiffer, T; Eixmann, T; Lohmann, S; Ahrens, M; Rehra, J; Draxinger, W; König, P; Huber, R and Gereon Hüttmann: High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography. Op Lett 43(18), pp. 4386-4389, 2018
    BibTeX Link
    @article{Schulz-Hildebrandt2018b,
    title = {High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography},
    author = { Schulz-Hildebrandt, H; Pfeiffer, T; Eixmann, T; Lohmann, S; Ahrens, M; Rehra, J; Draxinger, W; K\"{o}nig, P; Huber, R and Gereon H\"{u}ttmann},
    url = {https://doi.org/10.1364/OL.43.004386},
    
    
    year = {2018},
    date = {2018-09-05},
    journal = {Op Lett} {43(18)},
    
    pages = {4386-4389},
    abstract = {We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 μm was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in spirals for scanning the sample, the beam quality remains constant over the entire field of view with a diameter of 0.8 mm. The scanning frequency was adjusted to 1.22 kHz for use with a 3.28 MHz Fourier domain mode locked OCT system. Densely sampled volumes have been imaged at a rate of 6 volumes per second.
    
    © 2018 Optical Society of America},
    keywords = {Endoskope, AG-Huber_OCT},
    pubstate = {published},
    tppubtype = {article}
    }
  • Smits, J; Ourak, M; Gijbels, A; Esteveny, L; Borghesan, G; Schoevaerdts, L; Willekens; Stalmans, P; Lankenau, E; Schulz-Hildebrandt, H; Hüttmann, G; Reynaerts, D and Vander Poorten, E.B: Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation. 2018 IEEE Intern Conf Robot a Automation (ICRA), pp. 129-134, 2018
    BibTeX Link
    @inproceedings{Smits2018,
    title = {Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation},
    author = {Smits, J; Ourak, M; Gijbels, A;  Esteveny, L; Borghesan, G; Schoevaerdts, L; Willekens; Stalmans, P; Lankenau, E; Schulz-Hildebrandt, H; H\"{u}ttmann, G; Reynaerts, D and  Vander Poorten, E.B},
    
    doi = {10.1109/ICRA.2018.8460983},
    year = {2018},
    date = {2018-09-20},
    journal = {2018 IEEE Intern Conf Robot a Automation (ICRA)},
    pages = {129-134},
    abstract = {Retinal Vein Occlusion is a common retinal vascular disorder which can cause severe loss of vision. Retinal vein cannulation followed by injection of an anti-coagulant into the affected vein is a promising treatment. However, given the scale and fragility of the surgical workfield, this procedure is considered too high-risk to perform manually. A first successful robot-assisted procedure has been demonstrated. Even though successful, the procedure remains extremely challenging. This paper aims at providing a solution for the limited perception of instrument-tissue interaction forces as well as depth estimation during retinal vein cannulation. The development of a novel combined force and distance sensing cannulation needle relying on Fiber Bragg grating (FBG) and Optical Coherence Tomography (OCT) A-scan technology is reported. The design, the manufacturing process, the calibration method, and the experimental characterization of the produced sensor are discussed. 
    The functionality of the combined sensing modalities and the real-time distance estimation algorithm are validated respectively on in-vitro and ex-vivo models.},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Ourak, M; Smits, J; Esteveny, L; Borghesan, G; Gijbels, A; Schoevaerdts, L; Douven, Y, Scholtes, J; Lankenau, E; Eixmann, T; Schulz-Hildebrandt, H; Hüttmann, G; Kozlovszky, M; Kronreif, G; Willekens, K; Stalmans, P; Faridpooya, K; Cereda, M; Giani, A; Staurenghi, G; Reynaerts, D and Vander Poorten, E B: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. Int J Comp Assis Radiol Surg 13(107), pp. 1-9, 2018
    BibTeX Link
    @article{Ourak2018,
    title = {Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation},
    author = {Ourak, M; Smits, J; Esteveny, L; Borghesan, G; Gijbels, A; Schoevaerdts, L;  Douven, Y, Scholtes, J; Lankenau, E;  Eixmann, T; Schulz-Hildebrandt, H; H\"{u}ttmann, G; Kozlovszky, M; Kronreif, G; Willekens, K; Stalmans, P; Faridpooya, K; Cereda, M; Giani, A; Staurenghi, G; Reynaerts, D and Vander Poorten, E B},
    
    doi = {10.1007/s11548-018-1829-0},
    year = {2018},
    date = {2018-07-28},
    journal = {Int J Comp Assis Radiol Surg} {13(107)},
    v
    pages = {1-9},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Schulz-Hildebrandt,H; Münter, M; Ahrens,M; Spahr, H; Hillmann, D; König, P and Hüttmann, G: Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations. in 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources, no. 10591, pp. 105910O, 2018
    BibTeX Link
    @inproceedings{Schulz-Hildebrandt2018a,
    title = {Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations},
    author = {Schulz-Hildebrandt,H; M\"{u}nter, M; Ahrens,M; Spahr, H; Hillmann, D; K\"{o}nig, P and  H\"{u}ttmann, G},
    doi = {10.1117/12.2303755},
    isbn = {9781510616745},
    year = {2018},
    date = {2018-03-05},
    booktitle = {2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources},
    volume = {10591},
    pages = {105910O},
    abstract = {Optical coherence tomography (OCT) images scattering tissues with 5 to 15 μm resolution. This is usually not sufficient for a distinction of cellular and subcellular structures. Increasing axial and lateral resolution and compensation of artifacts caused by dispersion and aberrations is required to achieve cellular and subcellular resolution. This includes defocus which limit the usable depth of field at high lateral resolution. OCT gives access the phase of the scattered light and hence correction of dispersion and aberrations is possible by numerical algorithms. Here we present a unified dispersion/aberration correction which is based on a polynomial parameterization of the phase error and an optimization of the image quality using Shannon’s entropy. For validation, a supercontinuum light sources and a costume-made spectrometer with 400 nm bandwidth were combined with a high NA microscope objective in a setup for tissue and small animal imaging. Using this setup and computation corrections, volumetric imaging at 1.5 μm resolution is possible. Cellular and near cellular resolution is demonstrated in porcine cornea and the drosophila larva, when computational correction of dispersion and aberrations is used. Due to the excellent correction of the used microscope objective, defocus was the main contribution to the aberrations. In addition, higher aberrations caused by the sample itself were successfully corrected. Dispersion and aberrations are closely related artifacts in microscopic OCT imaging. Hence they can be corrected in the same way by optimization of the image quality. This way microscopic resolution is easily achieved in OCT imaging of static biological tissues.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {inproceedings}
    }

2017

  • Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Richard Neffin and Peter Koch and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten: Probabilistic Principal Component Analysis and Particle Filtering for real-time retina detection from a single-fiber OCT. in Proceedings of the 7th Joint Workshop on New Technologies for Computer/Robot Assisted Surgery, 2017
    BibTeX
    @conference{Borghesan2017,
    title = {Probabilistic Principal Component Analysis and Particle Filtering for real-time retina detection from a single-fiber OCT},
    author = {Gianni Borghesan and Mouloud Ourak and Eva Lankenau and Richard Neffin and Peter Koch and Hinnerk Schulz-Hildebrandt and Koen Willekens and Peter Stalmans and Dominiek Reynaerts and Emmanuel Vander Poorten},
    year = {2017},
    date = {2017-06-02},
    booktitle = {Proceedings of the 7th Joint Workshop on New Technologies for Computer/Robot Assisted Surgery},
    abstract = {Vitreo-retinal surgery concerns a set of particularly demanding micro-surgical interventions that take place at the back of the eye. Examples of such procedures are retinal vein cannulation (where the surgeon aims to insert a needle in a vein of the size of human hairs) and epiretinal membrane peeling (where a detached membrane must be removed from the retina). As severe retinal damage can be caused by undesired collisions, good instrument to retina distance perception would be very useful. We propose to use an OCT-fiber instrumented tool, and purposefully designed algorithms to interpret the measurements and extract a reliable real-time distance estimate. This abstract describes the progress that was made and includes a test conducted with a robotic platform on a synthetic eye mockup.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Horstmann, J; Schulz-Hildebrandt, H; Bock, F; Siebelmann, S; Lankenau, E; Hüttmann, G; Steven, P and Cursiefen, C: Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography. Investig Ophthal & Vis Scie 58(13), pp. 5880-5886, 2017
    BibTeX Link
    @article{Horstmann2017,
    title = {Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography},
    author = {Horstmann, J; Schulz-Hildebrandt, H; Bock, F; Siebelmann, S; Lankenau, E; H\"{u}ttmann, G; Steven, P and  Cursiefen, C},
    editor = {The Association Research for in Vision and Ophthalmology},
    doi = {10.1167/iovs.17-22286},
    isbn = {1552-5783},
    year = {2017},
    date = {2017-12-25},
    journal = { Investig Ophthal & Vis Scie} {58(13)},
    
    pages = {5880-5886},
    abstract = {Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). 
    
    Methods: Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were acquired using a custom-built mOCT system enabling a resolution of 1 μm at a B-scan rate of 165/s. Three of the six animals received an additional intrastromal injection of India ink 24 hours before the measurement to stain the corneal lymphatic system in vivo. Immunohistochemistry using CD31 and LYVE-1 was used to validate the mOCT findings. 
    
    Results: Using mOCT, lymphatic vessels were visible as dark vessel-like structures with the lumen lacking a hyperreflective wall and mostly lacking cells. However, individual, slowly moving particles, which most likely are immune cells, occasionally could be observed inside the lumen. In lymphatic vessels of ink-stained corneas, hyperreflection and shadowing underneath was observed. Ink-filled lymphatic vessels were colocalized in consecutive corneal flat mounts of the same specimen. 
    
    Conclusions: Corneal lymphatic vessels can be imaged using mOCT. This novel approach opens new options for noninvasive clinical imaging of corneal lymphatic vessels for diagnostic and therapeutic indications.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon Hüttmann and Dieter Manstein: Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation). in Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery, no. 10037OS, 2017
    BibTeX Link
    @conference{Casper2017,
    title = {Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)},
    author = {Malte Casper and Hinnerk Schulz-Hildebrandt and Michael Evers and Cuc Nguyen and Reginald Birngruber and Gereon H\"{u}ttmann and Dieter Manstein},
    editor = {Spie},
    doi = {doi: 10.1117/12.2251485},
    year = {2017},
    date = {2017-04-19},
    booktitle = {Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery},
    volume = {10037OS},
    abstract = {In dermatology the reflexes of vasoconstriction and vasodilation are known as important mechanisms of thermoregulation of the inner body. Imaging the physiology of microvasculature of the skin with high spatial resolution in three dimensions while reacting to changes in temperature is crucial for understanding the complex processes of vasodynamics, which result in constriction and dilation of vessels. However, previous studies using Laser-Doppler flowmetry and -imaging could not provide reliable angiographic images which allow to quantify changes in blood vessel diameter. Here, we report a different approach for angiographic imaging of microvasculature of a anaesthetized rodent model using speckle variance optical coherence tomography (svOCT) during and after localized cooling. Therefore a commercial OCT with a center wavelength of 1.3 μm and a spatial resolution of 13µm was used in combination with a custom built cooling device to image such reflexes at the mouse ear pinna and dorsal skinfold. Cooling was applied in steps of 2−5◦ C starting at the baseline temperature of 27◦ C down to −10◦ C. To our surprise and in contrast to the general opinion in literature, we were able to observe that the majority of vessels with a diameter larger than 20 μm maintain perfused with a constant diameter when the tissue is cooled from baseline to subzero temperatures. However, vasoconstriction was observed very rarely and only in veins, which led to their occlusion. The results of this experiment lead us to reconsider essential aspects of previous understanding of temperature-induced vasodynamics in cutaneous microvasculature.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Latus, S; Otte, C; Schlüter, M; Rehra,J; Bizon, K; Schulz-Hildebrandt, H; Saathoff, T; Hüttmann, Gereon and Schlaefer, A: An Approach for Needle Based Optical Coherence Elastography Measurements. in Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017 10434, pp. 655-663, 2017
    BibTeX Link
    @conference{Latus2017,
    title = {An Approach for Needle Based Optical Coherence Elastography Measurements},
    author = {Latus, S; Otte, C; Schl\"{u}ter, M; Rehra,J; Bizon, K; Schulz-Hildebrandt, H; Saathoff, T; H\"{u}ttmann, Gereon and Schlaefer, A},
    editor = {Springer International Publishing},
    url = {https://link.springer.com/chapter/10.1007/978-3-319-66185-8_74},
    isbn = {978-3-319-66185-8},
    year = {2017},
    date = {2017-09-01},
    booktitle = {Medical Image Computing and Computer-Assisted Intervention − MICCAI 2017} {10434},
    
    pages = {655--663},
    organization = {Springer},
    abstract = {© Springer International Publishing AG 2017. 
    While navigation and interventional guidance are typically based on image data, the images do not necessarily reflect mechanical tissue properties. Optical coherence elastography (OCE) presents a modality with high sensitivity and very high spatial and temporal resolution. However, OCE has a limited field of view of only 2\textendash5 mm depth. We present a side-facing needle probe to image externally induced shear waves from within soft tissue. A first method of quantitative needle-based OCE is provided. Using a time of flight setup, we establish the shear wave velocity and estimate the tissue elasticity. For comparison, an external scan head is used for imaging. Results for four different phantoms indicate a good agreement between the shear wave velocities estimated from the needle probe at different depths and the scan head. The velocities ranging from 0.9\textendash3.4 m/s agree with the expected values, illustrating that tissue elasticity estimates from within needle probes are feasible.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }

2016

  • Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.: Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. 1-48, 2016
    BibTeX Link
    @article{Schulz-Hildebrandt2016,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.},
       title = {Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {1-48},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584651},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 2: Praktische Aspekte und Anwendung. Augenheilkunde up2date, no. 6, pp. 305-320, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 2: Praktische Aspekte und Anwendung},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {305-320},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-117459},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 1: Physikalische Grundlagen. Augenheilkunde up2date, no. 6, pp. 289-300, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 1: Physikalische Grundlagen},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {289-300},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-113337},
       year = {2016},
       type = {Journal Article}
    }
    
  • Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.: Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. SOP2, 2016
    BibTeX Link
    @article{Pieper2016,
       author = {Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.},
       title = {Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {SOP2},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584654},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.: Improved endoscopic optical coherence microscopy for imaging of humans airways in patients. Pneumologie, no. 69, pp. A49, 2015
    BibTeX Link
    @article{Schulz-Hildebrandt2015,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.},
       title = {Improved endoscopic optical coherence microscopy for imaging of humans airways in patients},
       journal = {Pneumologie},
       volume = {69},
       number = {07},
       pages = {A49},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0035-1556641},
       year = {2015},
       type = {Journal Article}
    }
    

2014

  • Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino: Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography. European Archives of Oto-Rhino-Laryngology, pp. 1-7, 2014
    BibTeX Link
    @article{Guder2014,
       author = {Guder, Ellen and Lankenau, Eva and Fleischhauer, F. and Schulz-Hildebrandt, H. and Hüttmann, G. and Pau, H. W. and Just, Tino},
       title = {Microanatomy of the tympanic membrane in chronic myringitis obtained with optical coherence tomography},
       journal = {European Archives of Oto-Rhino-Laryngology},
       pages = {1-7},
       keywords = {Optical coherence tomography
    Tympanic membrane
    Chronic myringitis},
       ISSN = {0937-4477},
       DOI = {10.1007/s00405-014-3373-z},
       url = {http://dx.doi.org/10.1007/s00405-014-3373-z},
       year = {2014},
       type = {Journal Article}
    }
    

  • Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon: Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination. in Studierendentagung, Universität zu Lübeck,
    BibTeX
    @inproceedings{Fleischhauer2013,
       author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon},
       title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings}
    }
    
  • Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter: Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation). no. 9691, pp. 969116-969116-1,
    BibTeX Link
    @inproceedings{Pieper2016,
       author = {Pieper, Mario and Schulz-Hildebrandt, Hinnerk and Hüttmann, Gereon and König, Peter},
       title = {Imaging of mucus clearance in the airways of living spontaneously breathing mice by optical coherence microscopy (Conference Presentation)},
       volume = {9691},
       pages = {969116-969116-1},
       note = {10.1117/12.2209054},
       abstract = {Mucus transport is essential to remove inhaled particles and pathogens from the lung. Impaired removal of mucus often results in worsening of lung diseases. To understand the mechanisms of mucus transport and to monitor the impact of therapeutic strategies, it is essential to visualize airways and mucus in living animals without disturbing transport processes by intubation or surgically opening the airways. We developed a custom-built optical coherence microscope (OCM) providing a lateral and axial resolution of approximately 1.5 µm with a field of view of 2 mm at up to 150 images/s. Images of the intact trachea and its mucus transport were recorded in anesthetized spontaneously breathing mice. NaCl solution (0.9% and 7%) or Lipopolysaccharide were applied intranasally. OCM resolved detailed structure of the trachea and enabled measuring the airway surface liquid (ASL) thickness through the tracheal wall. Without stimulation, the amount of ASL was only a few µm above the epithelium and remained constant. After intranasal application of 30 µl saline at different concentrations, an early fast cough-like fluid removal with velocities higher than 1 mm/s was observed that removed a high amount of liquid. The ASL thickness increased transiently and quickly returned to levels before stimulation. In contrast to saline, application of Lipopolysaccharide induced substantial mucus release and an additional slow mucus transport by ciliary beating (around 100 µm/s) towards the larynx was observed. In conclusion, OCM is appropriate unique tool to study mechanisms of mucus transport in the airways and effects of therapeutic interventions in living animals.},
       url = {http://dx.doi.org/10.1117/12.2209054},
       type = {Conference Proceedings}
    }