Publikationen 2018 - 2019

2019

  • Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R and Popp, J: Virtual HE histology by fiber-based picosecond two-photon microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIX Proc. SPIE,10882, pp. 108822F, International Society for Optics and Photonics, 2019
    BibTeX Link
    @inproceedings{Kolb2019/2,
       author = {Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R and Popp, J},
       title = {Virtual HE histology by fiber-based picosecond two-photon microscopy},
       booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX} {Proc. SPIE,10882},
       editor = {Ammasi Periasamy; Peter T. C. So; Karsten König},
       publisher = { International Society for Optics and Photonics},
       year = {2019},
       pages = {108822F},
    keywords = {AG-Huber_NL},
       
    doi = {10.1117/12.2507866},
       type = {Conference Proceedings}
    }
    
  • Miura, Y;Seifert, E;Rehra, J;Kern, K;Theisen-Kunde, D;Denton, M and Brinkmann, R: Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study. Int J Hyperth, pp. 1-7, 2019
    BibTeX Link
    @article{Miura2019/4,
       author = {Miura, Y;Seifert, E;Rehra, J;Kern, K;Theisen-Kunde, D;Denton, M and Brinkmann, R},
       title = {Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study},
       journal = {Int J Hyperth},
       pages = {1-7},
       ISSN = {0265-6736},
      
       url = {https://doi.org/10.1080/02656736.2019.1590653},
       year = {2019},
       type = {Journal Article}
    }
    
  • Klettner, A and Miura, Y: Porcine RPE/choroidal explant cultures. Weber B., Langmann T., Retinal Degeneration. Methods in Molecular Biology: 1834, pp. 109-118, 2019
    BibTeX Link
    @book2019{Miura2019,
       author = {Klettner, A and  Miura, Y},
       title = {Porcine RPE/choroidal explant cultures},
       pages = {109-118 },
    journal = {Weber B., Langmann T., Retinal Degeneration. Methods in Molecular Biology: 1834},
       ISBN = {978-1-4939-8669-2},
      URL = {https://doi.org/10.1007/978-1-4939-8669-9_8},
       year = {2019},
       type = {Book}
    }
    
  • Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber,R; Manstein, D and Hüttmann, G: Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography. J Biomed Opt 24(4), pp. 046005 - 1 - 11, 2019
    BibTeX Link
    @article{
    citation_key = { Casper19}
    author = { Casper, M;  Schulz-Hildebrandt, H; Evers, M; Birngruber,R; Manstein, D and Hüttmann, G},
    title = {Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography},
    
    journal = {J Biomed Opt} {24(4)},
    
    pages = {046005 - 1 - 11},
    year = {2019},
    
    URL = {https://doi.org/10.1117/1.JBO.24.4.046005},
    eprint = {}
    }
  • Liang, X-X; Zhang, Z and Vogel, A: Multi-rate-equation modeling of the energy spectrum of laser-induced conduction band electrons in water. Opt Expr 27(4), pp. 672-4693, 2019
    BibTeX Link
    @article{Liang2019,
       author = {Liang, X-X; Zhang, Z and Vogel, A},
       title = {Multi-rate-equation modeling of the energy spectrum of laser-induced conduction band electrons in water},
       journal = {Opt Expr} { 27(4)},
       
       
       pages = {4672-4693}
       DOI = {10.1364/OE.27.004672}
       
       year = {2019},
       type = {Journal Article}
    }
    
  • South,F A; Liu, Y-Z; Huang, P-C and Kohlfärber, T and Boppart, S A: Local wavefront mapping in tissue using computational adaptive optics OCT. Opt Lett 44(5), pp. 1186-1189, OSA, 2019
    BibTeX Link
    @article{South:19,
    author = { South,F A; Liu, Y-Z; Huang, P-C and Kohlfärber, T and Boppart, S A},
    journal = { Opt Lett 44(5)}
    pages = {1186--1189},
    keywords = {Adaptive optics; Image metrics; Image quality; Refractive index; Speckle noise; Wavefront aberrations},
    number = {5},
     
    publisher = {OSA},
    title = {Local wavefront mapping in tissue using computational adaptive optics OCT},
    
    
    year = {2019},
    doi = {10.1364/OL.44.001186},
    abstract = {The identification and correction of wavefront aberrations is often necessary to achieve high-resolution optical images of biological tissues, as imperfections in the optical system and the tissue itself distort the imaging beam. Measuring the localized wavefront aberration provides information on where the beam is distorted and how severely. We have recently developed a method to estimate the single-pass wavefront aberrations from complex optical coherence tomography (OCT) data. Using this method, localized wavefront measurement and correction using computational OCT was performed in ex vivo tissues. The computationally measured wavefront varied throughout the imaged OCT volumes and, therefore, a local wavefront correction outperformed a global wavefront correction. The local wavefront measurement was also used to generate tissue aberration maps. Such aberration maps could potentially be used as a new form of tissue contrast.},
    }
    
  • Kolb, J P;Draxinger, W;Klee, J;Pfeiffer, T;Eibl, M;Klein, T;Wieser, W and Huber, R: Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates. J pone 14(3), pp. e0213144, 2019
    BibTeX Link
    @article{Kolb2019,
       author = {Kolb, J P;Draxinger, W;Klee, J;Pfeiffer, T;Eibl, M;Klein, T;Wieser, W and Huber, R},
       title = {Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates},
       journal = {J pone} {14(3)},
     keywords = {AG-Huber_OCT},
       url = {https://doi.org/10.1371/journal.pone.0213144},
       pages = {e0213144},
       ISSN = {1932-6203},
       
       year = {2019},
       type = {Journal Article}
    }
    
  • Karpf, S and Jalali, B: Fourier-domain mode-locked laser combined with a master-oscillator power amplifier architecture. J Opt Lett 44(8), pp. 1952-1955, 2019
    BibTeX Link
    @article{Karpf2019,
       author = {Karpf, S and Jalali, B },
       title = {Fourier-domain mode-locked laser combined with a master-oscillator power amplifier architecture},
       journal = {J Opt Lett} {44(8)},
       URL = {https://doi.org/10.1364/OL.44.001952},
       pages = {1952-1955},
       ISSN = {1539-4794},
       year = {2019},
     keywords = {AG-Huber},
       type = {Journal Article}
    }
    
  • Richert, E;Bartsch, S;Hillenkamp, J;Treumer, F;Tode, J;von der Burchard, C;Brinkmann, R;Klettner, A K and Roider, J: Einfluss der Selektiven Retinatherapie (SRT) auf inflammatorische Zellmediatoren des subretinalen Raums. Klin Monatsbl Augenheilkd(EFirst), 2019
    BibTeX Link
    @article{Brinkmann2019-4,
       author = {Richert, E;Bartsch, S;Hillenkamp, J;Treumer, F;Tode, J;von der Burchard, C;Brinkmann, R;Klettner, A K and Roider, J},
       title = {Einfluss der Selektiven Retinatherapie (SRT) auf inflammatorische Zellmediatoren des subretinalen Raums},
       journal = {Klin Monatsbl Augenheilkd}{(EFirst)},
    URL = { https://doi.org/10.1055/a-0838-5633}
       ISSN = {0023-2165},
       Year = {2019},
       type = {Journal Article}
    }
    
  • Freidank, S;Vogel, A;Anderson, R. R.;Birngruber, R and Linz, N: Correction of hyperopia by intrastromal cutting and liquid filler injection. J Biomed Opt 24 (5), pp. 1-7, 7, 2019
    BibTeX Link
    @article{Freidank2019,
       author = {Freidank, S;Vogel, A;Anderson, R. R.;Birngruber, R and Linz, N},
       title = {Correction of hyperopia by intrastromal cutting and liquid filler injection},
       journal = {J Biomed Opt} {24 (5)},
      
       number = {5 %J Journal of Biomedical Optics},
       pages = {1-7, 7},
       
       url = {https://doi.org/10.1117/1.JBO.24.5.058001},
       year = {2019},
       type = {Journal Article}
    }
    
  • Ourak, M. Smits, J. Esteveny, L. Borghesan, G. Gijbels, A. Schoevaerdts, L. Douven, Y. Scholtes, J. Lankenau, E. Eixmann, T. Schulz-Hildebrandt, H. Hüttmann, G. Kozlovszky, M. Kronreif, G. Willekens, K. Stalmans, P. Faridpooya, K. Cereda, M. Giani, A. Staurenghi, G. Reynaerts, D. Vander Poorten, E. B.: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. International Journal of Computer Assisted Radiology and Surgery, no. 14, pp. 301-309, Feb, 2019
    BibTeX Link Link
    @Article{Ourak2019,
    author="Ourak, M.
    and Smits, J.
    and Esteveny, L.
    and Borghesan, G.
    and Gijbels, A.
    and Schoevaerdts, L.
    and Douven, Y.
    and Scholtes, J.
    and Lankenau, E.
    and Eixmann, T.
    and Schulz-Hildebrandt, H.
    and H{\"u}ttmann, G.
    and Kozlovszky, M.
    and Kronreif, G.
    and Willekens, K.
    and Stalmans, P.
    and Faridpooya, K.
    and Cereda, M.
    and Giani, A.
    and Staurenghi, G.
    and Reynaerts, D.
    and Vander Poorten, E. B.",
    title="Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation",
    journal="International Journal of Computer Assisted Radiology and Surgery",
    year="2019",
    month="Feb",
    day="01",
    volume="14",
    number="2",
    pages="301--309",
    abstract="Retinal vein cannulation is an experimental procedure during which a clot-dissolving drug is injected into an obstructed retinal vein. However, due to the fragility and minute size of retinal veins, such procedure is considered too risky to perform manually. With the aid of surgical robots, key limiting factors such as: unwanted eye rotations, hand tremor and instrument immobilization can be tackled. However, local instrument anatomy distance and force estimation remain unresolved issues. A reliable, real-time local interaction estimation between instrument tip and the retina could be a solution. This paper reports on the development of a combined force and distance sensing cannulation needle, and its experimental validation during in vivo animal trials.",
    issn="1861-6429",
    doi="10.1007/s11548-018-1829-0",
    url="https://doi.org/10.1007/s11548-018-1829-0"
    citation_key = {Ourak2019}
    }
  • Miura, Y;Lewke, B;Hutfilz, A and Brinkmann, R: Change in fluorescence lifetime of retinal pigment epithelium under oxidative stress. Nippon Ganka Gakkai Zasshi 123 (2), pp. 105-114, 2019
    BibTeX Link
    @article{Miura2019/3,
       
       author = {Miura, Y;Lewke, B;Hutfilz, A and Brinkmann, R},
       title = {Change in fluorescence lifetime of retinal pigment epithelium under oxidative stress},
       journal = {Nippon Ganka Gakkai Zasshi } {123 (2)},
      
       pages = {105-114},
       url = {http://journal.nichigan.or.jp/Disp?style=abst&vol=123&year=2019&mag=0&number=2&start=105},
       year = {2019},
       type = {Journal Article}
    }
    
    
  • Spahr, H; Pfäffle, C; Huettmann, G and Hillmann, D: Artifacts in speckle tracking and multi-aperture Doppler OCT imaging of lateral motion. Opt Lett 44(5), pp. 1315-1318, 2019
    BibTeX Link
    @article{Spahr2019,
       author = {Spahr, H; Pfäffle, C; Huettmann, G and Hillmann, D},
       title = { Artifacts in speckle tracking and multi-aperture
    Doppler OCT imaging of lateral motion},
       journal = { Opt Lett} {44(5)},
       pages = {1315-1318},
    URL = {https://doi.org/10.1364/OL.44.001315},
       year = {2019},
       type = {Journal Article}
    }
    
  • Rudolf, M; Curcio, C A; Schlözer-Schrehardt, U; Sefat, A M M; Tura, A; Aherrahrou, Z; Brinkmann, M; Grisanti, S; Miura, Y and Ranjbar, M: Apolipoprotein A-I mimetic peptide L-4F removes Bruch`s membrane lipids in aged nonhuman primates. Invest Ophthalmol Vis Sci, pp. 461-472, 2019
    BibTeX Link
    @article{Miura2019-2,
       author = {Rudolf, M; Curcio, C A; Schlözer-Schrehardt, U; Sefat, A M M; Tura, A; Aherrahrou, Z; Brinkmann, M; Grisanti, S;  Miura, Y and Ranjbar, M},
       title = {Apolipoprotein A-I mimetic peptide L-4F removes Bruch's membrane lipids in aged nonhuman primates},
       journal = {Invest Ophthalmol Vis Sci},
       pages = {461-472},
      
       url = {https://www.ncbi.nlm.nih.gov/pubmed/30707219},
       year = {2019},
       type = {Journal Article}
    }
    

2018

  • Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S: Wavelength agile multi-photon microscopy with a fiber amplified diode laser. Biomedical Optics Express 9(12), pp. 6273-6282, 2018
    BibTeX Link
    @article{Eibl2018,
    author = { Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S},
    title = {Wavelength agile multi-photon microscopy with a fiber amplified diode laser},
    journal = {Biomedical Optics Express} {9(12)},
    keywords = {AG-Huber_NL},
    year = {2018},
    pages = {6273--6282},
    doi = {10.1364/BOE.9.006273},
    }
    
  • Pfeiffer, T; Petermann, M; Draxinger, W; Jirauschek, C and Huber, R: Ultra low noise Fourier domain mode locked laser for high quality magahertz optical coherence tomography. Biomed Opt Expr 9(9), pp. 4130-4148, 2018
    BibTeX Link
    @article{Pfeiffer2018,
       author = {Pfeiffer, T; Petermann, M; Draxinger, W; Jirauschek, C and Huber, R},
       title = {Ultra low noise Fourier domain mode locked laser for high quality magahertz optical coherence tomography},
       journal = {Biomed Opt Expr} {9(9)},
      
       pages = {4130-4148},
     DOI = {10.1364/BOE.9.004130},  
    
       year = {2018},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
       type = {Journal Article}
    }
    
  • Miura, Y: Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo. in Mouse Retinal Phenotyping: Methods and Protocols, pp. 73-88, Springer New York, New York, NY, 2018
    BibTeX Link
    @inbook{Miura2018,
       author = {Miura, Y},
       title = {Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo},
       booktitle = {Mouse Retinal Phenotyping: Methods and Protocols},
       editor = {Tanimoto, Naoyuki},
       publisher = {Springer New York},
      
       pages = {73-88},
       ISBN = {978-1-4939-7720-8},
       url={https://doi.org/10.1007/978-1-4939-7720-8_5},
       year = {2018},
       type = {Book Section}
    }
    
  • Tode, J; Richert, E; Koinzer, S; Klettner, A; von der Burchard, C; Brinkmann, R; Lucius, R and Roider, J: Thermal Stimulation of the Retina Reduces Bruch`s Membrane Thickness in Age Related Macular Degeneration Mouse Models. Transl Vis Sci Technol 7(3), pp. 2, 2018
    BibTeX Link
    @article{Tode2018,
       author = {Tode, J; Richert, E; Koinzer, S; Klettner, A; von der Burchard, C; Brinkmann, R; Lucius, R and Roider, J},
       title = {Thermal Stimulation of the Retina Reduces Bruch's Membrane Thickness in Age Related Macular Degeneration Mouse Models},
       journal = {Transl Vis Sci Technol} {7(3)},
      
       pages = {2},
       ISSN = {2164-2591 (Print)
    2164-2591 (Linking)}, 
       url = {https://www.ncbi.nlm.nih.gov/pubmed/29736323},
       year = {2018},
       type = {Journal Article}
    }
    
  • Herzog C;Thompson O; Schmarbeck B; Siebert M and Brinkmann, R: Temperature-controlled laser therapy of the retina via robust adaptive Ɦ∞-control. at-Automatisierungstechnik 66 (12), pp. 1051-1063, De Gruyter, 2018
    BibTeX Link
       @book{Brinkmann2018/2,
       author = {Herzog, C;Thompson, O; Schmarbeck, B; Siebert, M and Brinkmann, R},
       title = {Temperature-controlled laser therapy of the retina via robust adaptive Ɦ∞-control},
       publisher = {De Gruyter},
       
       journal = {at-Automatisierungstechnik} {66 (12)},
       pages = {1051-1063},   
       year = {2018},
       type = {Book}
      URL = {https://doi.org/10.1515/auto-2018-0066},
       
      
    keywords = {Laser therapy; robust control; parameter estimation; photoacoustics; real-time temperature determination},
       abstract = {Recent studies demonstrate therapeutic benefits in retinal laser therapy even for non-visible effects of the irradiation. However, in practice, ophthalmologists often rely on the visual inspection of irradiation sites to manually set the laser power for subsequent ones. Since absorption properties vary strongly between sites, this procedure can lead to under- or over-treatment. To achieve safe automatic retinal laser therapy, this article proposes a robust control scheme based on photoacoustic feedback of the retinal temperature increase. The control scheme is further extended to adapt to real-time parameter estimates and associated bounds on the uncertainty of each irradiation site. Both approaches are successfully validated in ex vivo experiments on pigs’ eyes, achieving consistent irradiation durations of 55 ms despite the uncertainty in absorption properties.}
    }
    
  • Borghesan,G; Ourak,M; Lankenau, E; Hüttmann,G; Schulz-Hildebrandt,H; Willekens,K; Stalmans, K; Reynaerts, D and Vander Poorten,E: Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation. J Med Robot Res 3(2), pp. 184005, 2018
    BibTeX Link Link
    @article{borghesan2018single,
    title = {Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation},
    author = {Borghesan,G; Ourak,M; Lankenau, E; H\"{u}ttmann,G; Schulz-Hildebrandt,H; Willekens,K; Stalmans, K; Reynaerts, D and Vander Poorten,E},
    editor = {World Scientific},
    url = {https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    doi = {10.1142/S2424905X18400056},
    year = {2018},
    date = {2018-02-12},
    journal = {J Med Robot Res} {3(2)},
    
    pages = {184005},
    abstract = {Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures or to inadvertently injure the underlying retina. Damage to the latter is mostly irreparable and might cause permanent loss of vision. Despite the availability over excellent stereo microscopes, wide-angle lenses and powerful light source visualization remains a problem. Especially, the limited depth perception is still perceived as a major bottle-neck whereas efforts have been conducted to integrate sensing capability in today’s state-of-the-art instruments, so far, little effort has been paid to process the obtained sensor data and turns this into a reliable source of information upon which robot assistive guidance schemes could be endowed upon. This paper proposes a number of processing techniques tailored to Optical Coherence Tomography (OCT) measurements. The first results of the proposed algorithms show that it is feasible to extract good and reliable distance estimates from this otherwise rather noisy signal and from a fairly limited dataset. The used data are the so-called A-scans. These are OCT measurements consisting of a single-line image that could be captured by an instrument-mounted fiber through which the OCT signal passes back-and-forth. However, in this work, we perform a pilot study whereby the employed A-scans are extracted from B-scans that are captured by a microscope-mounted OCT scanner, rather than obtained from a probe. The performance of a first embodiment of the algorithm that is based on an Unscented Kalman Filter (UKF) is compared to the performance of a second embodiment that relies on a Particle Filter (PF), focusing on the issues in filter initialization and the tracking quality. Finally, results of UKF and PF executions on a validation dataset are presented. 
    Read More: https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Sudkamp, H; Hillmann, D; Koch, P;vom Endt, M; Spahr, H; Münst, M; Pfäffle, C; Birngruber, R and Hüttmann, G: Simple approach for aberration-corrected OCT imaging of the human retina. Opt Lett 43(17), pp. 4224, 2018
    BibTeX Link
    @article{Sudkamp2018a,
       author = {Sudkamp, H; Hillmann, D; Koch, P;vom Endt, M; Spahr, H; Münst, M; Pfäffle, C; Birngruber, R and Hüttmann, G},
       title = {Simple approach for aberration-corrected OCT imaging of the human retina},
       journal = {Opt Lett} {43(17)},
       
       pages = {4224},
       ISSN = {0146-9592
    1539-4794},
       DOI = {10.1364/ol.43.004224},
       year = {2018},
       type = {Journal Article},
       keyword = {Retome}
    }
    
  • Seifert, E; Tode, J; Pielen, A; Theisen-Kunde, D; Framme, C; Roider, J; Miura, Y; Birngruber, R and Brinkmann, R: Selective retina therapy: toward an optically controlled automatic dosing. J Biomed Opt 23(11), pp. 1-12, 2018
    BibTeX Link
    @article{seifert2018,
       author = {Seifert, E; Tode, J; Pielen, A; Theisen-Kunde, D; Framme, C; Roider, J; Miura, Y; Birngruber, R and Brinkmann, R},
       title = {Selective retina therapy: toward an optically controlled automatic dosing},
       journal = {J Biomed Opt} {23(11)},
       
       pages = {1-12},
       ISSN = {1560-2281 (Electronic)
    1083-3668 (Linking)},
       DOI = {10.1117/1.JBO.23.11.115002},   
    keywords = {algorithm, lasers in medicine, ophthalmology, retinal pigment epithelium, selective retina therapy, selectivity}
       year = {2018},
       type = {Journal Article}
    }
    
  • Richert, E; Koinzer, S; Tode, J;Schlott, K; Brinkmann, R; Hillenkamp, J; Klettner, A and Roider, J: Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy. Invest Ophthal Vis Scie 59(3), pp. 1323-1331, 2018
    BibTeX Link
    @article{Richert2018,
       author = {Richert, E; Koinzer, S; Tode, J;Schlott, K; Brinkmann, R; Hillenkamp, J; Klettner, A and Roider, J},
       title = {Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy},
       journal = {Invest Ophthal Vis Scie} {59(3)},
       
       pages = {1323-1331},
       ISSN = {1552-5783},
       DOI = {10.1167/iovs.17-23163},
       
       year = {2018},
       type = {Journal Article}
    }
    
  • Richert, E; Koinzer, S; Tode, J; Schlott, K; Brinkmann, R; Hillenkamp, J; Klettner, A and Roider, J: Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy. Investigative Ophthalmology & Visual Science 59(3), pp. 1323-1331, 2018
    BibTeX Link
    @article{Brinkmann2018,
       author = {Richert, E; Koinzer, S; Tode, J; Schlott, K; Brinkmann, R; Hillenkamp, J; Klettner, A and Roider, J},
       title = {Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy},
       journal = {Investigative Ophthalmology & Visual Science} {59(3)},
       
       pages = {1323-1331},
       ISSN = {1552-5783},
      
       url = {http://dx.doi.org/10.1167/iovs.17-23163},
       year = {2018},
       type = {Journal Article}
    }
    
  • Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber, R; Manstein, D and Hüttmann, G: Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation). Proc.SPIE 10467, in Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 2018
    BibTeX Link Link
    @conference{Casper2018,
    title = {Optimized segmentation and characterization of capillary networks using OCT (Conference Presentation)},
    author = {Casper, M; Schulz-Hildebrandt, H; Evers, M; Birngruber, R; Manstein, D and H\"{u}ttmann, G },
    url = {https://doi.org/10.1117/12.2292005},
    doi = {10.1117/12.2292005},
    year = {2018},
    date = {2018-03-14},
    booktitle = {Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018},
    journal = {Proc.SPIE} {10467},
    
    abstract = {The ability to image the physiology of microvasculature with high spatial resolution in three dimensions while investigating structural changes of skin, is essential for understanding the complex processes of skin aging, wound healing and disease development. Further, the quantitative, automatic assessment of these changes enables to analyze large amounts of image data in an abstract but comprehensive manner. 
    However, previous work using OCT with methods of angiography was imaging less scattering, hence more challenging tissue than skin, such as brain and retina tissue. The published methods for capillary segmentation were mostly non-automatic, poorly benchmarked against state-of-the-art methods of computer vision and not applied to investigate medical processes and studies in a comprehensive fashion. 
    Here, segmentation of capillaries in skin is reported and its efficacy is demonstrated in both, a 
    longitudinal mouse study and a preliminary study in humans. By combining state-of-the-art image 
    processing methods in an optimized way, we were able to improve the segmentation results and analyze the impact of each post-processing step. 
    Furthermore, this automatic segmentation enabled us to analyze big amounts of 
    datasets automatically and derive meaningful conclusions for the planning of clinical studies. 
    With this work, optical coherence tomography is combined with methods of computer vision to a diagnostic 
    tool with unique capabilities to characterize vascular diversity and provide extraordinary 
    opportunities for dermatological investigation in both, clinics and research.},
    keywords = {},
    pubstate = {published},
    tppubtype = {conference}
    }
  • Schulz-Hildebrandt, H; Pieper, M; Stehmar,C; Ahrens, M; Idel, C; Wollenberg,B; König,P and Gereon Hüttmann: Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography. Biomedical Optics Express, pp. 636-647, 2018
    BibTeX Link Link
    @article{schulz2018novel,
    title = {Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography},
    author = {Schulz-Hildebrandt, H; Pieper, M; Stehmar,C; Ahrens, M; Idel, C; Wollenberg,B; K\"{o}nig,P and Gereon H\"{u}ttmann},
    editor = {Optic Socie Amer} {9(2)},
    url = {https://www.osapublishing.org/abstract.cfm?URI=boe-9-2-636
    https://www.osapublishing.org/boe/viewmedia.cfm?uri=boe-9-2-636&seq=0},
    doi = {10.1364/BOE.9.000636},
    isbn = {10.1364/BOE.9.000636},
    year = {2018},
    date = {2018-01-16},
    journal = {Biomedical Optics Express},
    
    pages = {636-647},
    abstract = {Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 \textendash 2 μm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests overall a better image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 μm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was determined successfully. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.},
    keywords = {Endoskope, OCM},
    pubstate = {published},
    tppubtype = {article}
    }
  • Rudnitzki, F; Feineis, S; Rahmanzadeh, R; Endl, E; Lutz, J; Groll, J and Huettmann, G: iRNA release from gold nanoparticles by nanosecond pulsed irradiation and analysis of the involved temperature increase. Journal of BIOPHOTONICS 11(9), 2018
    BibTeX Link
    @article{Rahmanzadeh2018,
       author = {Rudnitzki, F; Feineis, S; Rahmanzadeh, R; Endl, E; Lutz, J; Groll, J and Huettmann, G},
       title = {siRNA release from gold nanoparticles by nanosecond pulsed
    laser irradiation and analysis of the involved temperature
    increase},
       journal = {Journal of BIOPHOTONICS} {11(9)},
      doi = {10.1002/jbio.201700329},
       
    
    keywords = {cavitation|cell manipulation|controlled release|gold nanoparticle bio-conjugates|laser nanoeffects},
    abstract = {Nanosecond pulsed laser irradiation can trigger a release of nucleic acids from gold nanoparticles, but the involved nanoeffects are not fully understood yet. Here we investigate the release of coumarin labeled siRNA from 15 to 30 nm gold particles after nanosecond pulsed laser irradiation. Temperatures in the particle and near the surface were calculated for the different radiant exposures. Upon irradiation with laser pulses of 4 nanosecond duration release started for both particle sizes at a calculated temperature increase of approximately 500 K. Maximum coumarin release was observed for 15 nm particles after irradiation with radiant exposure of 80 mJ cm−2 and with 32 mJ cm−2 for 30 nm particles. This corresponds to a temperature increase of 815 and 900 K, respectively. Our results show that the molecular release by nanosecond pulsed irradiation is based on a different mechanism compared to continuous or femtosecond irradiation. Local temperatures are considerably higher and it is expected that bubble formation plays a crucial role in release and damage to cellular structures. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim}
       year = {2018},
       type = {Journal Article}
    }
    
  • Spahr, H; Pfäffle, C; Koch,C; Sudkamp, H; Hüttmann, G und Hillmann, D: Interferometric detection of 3D motion using computational subapertures in optical coherence tomography. Opt. Expr 15(26), pp. 18803-18816, OSA, 2018
    BibTeX Link
    @article{Spahr:18,
    author = { Spahr, H; Pfäffle, C; Koch,C; Sudkamp, H; Hüttmann, G und Hillmann, D},
    journal = {Opt Expr} {15(26)},
    keywords = {Funktion, Fullfield},
    
    pages = {18803--18816},
    publisher = {OSA},
    title = {Interferometric detection of 3D motion using computational subapertures in optical coherence tomography},
    
    
    year = {2018},
    url = {https://doi.org/10.1364/OE.26.018803},
    
    abstract = {Doppler optical coherence tomography (OCT) quantifies axial motion with high precision, whereas lateral motion cannot be detected by a mere evaluation of phase changes. This problem was solved by the introduction of three-beam Doppler OCT, which, however, entails a high experimental effort. Here, we present the numerical analogue to this experimental approach. Phase-stable complex-valued OCT datasets, recorded with full-field swept-source OCT, are filtered in the Fourier domain to limit imaging to different computational subapertures. These are used to calculate all three components of the motion vector with interferometric precision. As known from conventional Doppler OCT for axial motion only, the achievable accuracy exceeds the actual imaging resolution by orders of magnitude in all three dimensions. The feasibility of this method is first demonstrated by quantifying micro-rotation of a scattering sample. Subsequently, a potential application is explored by recording the 3D motion vector field of tissue during laser photocoagulation in ex-vivo porcine retina.},
    }
    
    
  • Schulz-Hildebrandt, H; Pfeiffer, T; Eixmann, T; Lohmann, S; Ahrens, M; Rehra, J; Draxinger, W; König, P; Huber, R and Gereon Hüttmann: High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography. Op Lett 43(18), pp. 4386-4389, 2018
    BibTeX Link
    @article{Schulz-Hildebrandt2018b,
    title = {High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography},
    author = { Schulz-Hildebrandt, H; Pfeiffer, T; Eixmann, T; Lohmann, S; Ahrens, M; Rehra, J; Draxinger, W; K\"{o}nig, P; Huber, R and Gereon H\"{u}ttmann},
    url = {https://doi.org/10.1364/OL.43.004386},
    
    
    year = {2018},
    date = {2018-09-05},
    journal = {Op Lett} {43(18)},
    
    pages = {4386-4389},
    abstract = {We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 μm was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in spirals for scanning the sample, the beam quality remains constant over the entire field of view with a diameter of 0.8 mm. The scanning frequency was adjusted to 1.22 kHz for use with a 3.28 MHz Fourier domain mode locked OCT system. Densely sampled volumes have been imaged at a rate of 6 volumes per second.
    
    © 2018 Optical Society of America},
    keywords = {Endoskope, AG-Huber_OCT},
    pubstate = {published},
    tppubtype = {article}
    }
  • Kolb, J P; Pfeiffer, T; Eibl, M; Hakert, H and Huber, R: High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates. Biomed Opt Expr 9(1), pp. 120-130, 2018
    BibTeX Link
    @article{Kolb2018,
       author = {Kolb, J P; Pfeiffer, T; Eibl, M; Hakert, H and Huber, R},
       title = {High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates},
       journal = {Biomed Opt Expr} {9(1)},
       
       pages = {120-130},
       DOI = {10.1364/BOE.9.000120},
       year = {2018},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
       type = {Journal Article}
    }
    
  • Kern, K; Mertineit, C L; Brinkmann, R and Miura, Y: Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells. Exp Eye Res 170, pp. 117-126, 2018
    BibTeX Link
    @article{Miura2018,
       author = {Kern, K; Mertineit, C L; Brinkmann, R and Miura, Y},
       title = {Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells},
       journal = {Exp Eye Res} {170},
      
       pages = {117-126},
       ISSN = {1096-0007 (Electronic)
    0014-4835 (Linking)},
       DOI = {10.1016/j.exer.2018.02.013},
       year = {2018},
       type = {Journal Article}
    }
    
  • Evers, M ;Salma, N; Osseiran, S; Casper, M; Birngruber, R; Evans, C L and Manstein, D: Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM. Scientific Reports 8(1), 2018
    BibTeX Link
    @article{Evers2018,
       author = {Evers, M ;Salma, N; Osseiran, S; Casper, M; Birngruber, R; Evans, C L and Manstein, D},
       title = {Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM},
       journal = {Scientific Reports} {8(1)},
       
       DOI = {10.1038/s41598-018-27093-x},
       
       year = {2018},
       type = {Journal Article}
    }
    
  • Smits, J; Ourak, M; Gijbels, A; Esteveny, L; Borghesan, G; Schoevaerdts, L; Willekens; Stalmans, P; Lankenau, E; Schulz-Hildebrandt, H; Hüttmann, G; Reynaerts, D and Vander Poorten, E.B: Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation. 2018 IEEE Intern Conf Robot a Automation (ICRA), pp. 129-134, 2018
    BibTeX Link
    @inproceedings{Smits2018,
    title = {Development and Experimental Validation of a Combined FBG Force and OCT Distance Sensing Needle for Robot-Assisted Retinal Vein Cannulation},
    author = {Smits, J; Ourak, M; Gijbels, A;  Esteveny, L; Borghesan, G; Schoevaerdts, L; Willekens; Stalmans, P; Lankenau, E; Schulz-Hildebrandt, H; H\"{u}ttmann, G; Reynaerts, D and  Vander Poorten, E.B},
    
    doi = {10.1109/ICRA.2018.8460983},
    year = {2018},
    date = {2018-09-20},
    journal = {2018 IEEE Intern Conf Robot a Automation (ICRA)},
    pages = {129-134},
    abstract = {Retinal Vein Occlusion is a common retinal vascular disorder which can cause severe loss of vision. Retinal vein cannulation followed by injection of an anti-coagulant into the affected vein is a promising treatment. However, given the scale and fragility of the surgical workfield, this procedure is considered too high-risk to perform manually. A first successful robot-assisted procedure has been demonstrated. Even though successful, the procedure remains extremely challenging. This paper aims at providing a solution for the limited perception of instrument-tissue interaction forces as well as depth estimation during retinal vein cannulation. The development of a novel combined force and distance sensing cannulation needle relying on Fiber Bragg grating (FBG) and Optical Coherence Tomography (OCT) A-scan technology is reported. The design, the manufacturing process, the calibration method, and the experimental characterization of the produced sensor are discussed. 
    The functionality of the combined sensing modalities and the real-time distance estimation algorithm are validated respectively on in-vitro and ex-vivo models.},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Han, J W; Choi, J; Kim, Y S, Kim, J; Brinkmann, R; Lyu, J and Park, T K: Comparison of the neuroinflammatory responses to selective retina therapy and continuous-wave laser photocoagulation in mouse eyes. Graefe`s Archive for Clinical and Experimental Ophthalmology 256(2), pp. 341-353, 2018
    BibTeX Link
    @article{Brinkmann2018,
       author = {Han, J W; Choi, J; Kim, Y S, Kim, J; Brinkmann, R; Lyu, J and Park, T K},
       title = {Comparison of the neuroinflammatory responses to selective retina therapy and continuous-wave laser photocoagulation in mouse eyes},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology} {256(2)},
       
       pages = {341-353},
     
    URL= {https://doi.org/10.1007/s00417-017-3883-7},
       year = {2018},
       type = {Journal Article}
    }
    
  • Ourak, M; Smits, J; Esteveny, L; Borghesan, G; Gijbels, A; Schoevaerdts, L; Douven, Y, Scholtes, J; Lankenau, E; Eixmann, T; Schulz-Hildebrandt, H; Hüttmann, G; Kozlovszky, M; Kronreif, G; Willekens, K; Stalmans, P; Faridpooya, K; Cereda, M; Giani, A; Staurenghi, G; Reynaerts, D and Vander Poorten, E B: Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation. Int J Comp Assis Radiol Surg 13(107), pp. 1-9, 2018
    BibTeX Link
    @article{Ourak2018,
    title = {Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation},
    author = {Ourak, M; Smits, J; Esteveny, L; Borghesan, G; Gijbels, A; Schoevaerdts, L;  Douven, Y, Scholtes, J; Lankenau, E;  Eixmann, T; Schulz-Hildebrandt, H; H\"{u}ttmann, G; Kozlovszky, M; Kronreif, G; Willekens, K; Stalmans, P; Faridpooya, K; Cereda, M; Giani, A; Staurenghi, G; Reynaerts, D and Vander Poorten, E B},
    
    doi = {10.1007/s11548-018-1829-0},
    year = {2018},
    date = {2018-07-28},
    journal = {Int J Comp Assis Radiol Surg} {13(107)},
    v
    pages = {1-9},
    keywords = {Endoskope},
    pubstate = {published},
    tppubtype = {article}
    }
  • Maertz, J; Kolb, J P; Klein, T; Mohler, K J; Eibl, M; Wieser, W; Huber, R; Priglinger, S and Wolf, A: Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm. Graefe`s Archive for Clinical and Experimental Ophthalmology 256(2), pp. 289-298, 2018
    BibTeX Link Link
    @article{Maertz2018,
       author = {Maertz, J; Kolb, J P; Klein, T; Mohler, K J; Eibl, M; Wieser, W; Huber, R; Priglinger, S and Wolf, A},
       title = {Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology} {256(2)},
       volume = ,
       number = {2},
       pages = {289-298},
       DOI = {10.1007/s00417-017-3857-9},
       url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032262413&doi=10.1007%2fs00417-017-3857-9&partnerID=40&md5=a46c315f12cf5e633ea0f7e644116eb3},
       year = {2018},
       Keywords= {En face imaging, Optical coherence tomography, Swept-source OCT, Megahertz OCT, 3D rendering, Optic disc, Optic disc pit, Optic disc pit maculopathy, AG-Huber_OCT}
       type = {Journal Article}
    }
  • Schulz-Hildebrandt,H; Münter, M; Ahrens,M; Spahr, H; Hillmann, D; König, P and Hüttmann, G: Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations. in 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources, no. 10591, pp. 105910O, 2018
    BibTeX Link
    @inproceedings{Schulz-Hildebrandt2018a,
    title = {Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations},
    author = {Schulz-Hildebrandt,H; M\"{u}nter, M; Ahrens,M; Spahr, H; Hillmann, D; K\"{o}nig, P and  H\"{u}ttmann, G},
    doi = {10.1117/12.2303755},
    isbn = {9781510616745},
    year = {2018},
    date = {2018-03-05},
    booktitle = {2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources},
    volume = {10591},
    pages = {105910O},
    abstract = {Optical coherence tomography (OCT) images scattering tissues with 5 to 15 μm resolution. This is usually not sufficient for a distinction of cellular and subcellular structures. Increasing axial and lateral resolution and compensation of artifacts caused by dispersion and aberrations is required to achieve cellular and subcellular resolution. This includes defocus which limit the usable depth of field at high lateral resolution. OCT gives access the phase of the scattered light and hence correction of dispersion and aberrations is possible by numerical algorithms. Here we present a unified dispersion/aberration correction which is based on a polynomial parameterization of the phase error and an optimization of the image quality using Shannon’s entropy. For validation, a supercontinuum light sources and a costume-made spectrometer with 400 nm bandwidth were combined with a high NA microscope objective in a setup for tissue and small animal imaging. Using this setup and computation corrections, volumetric imaging at 1.5 μm resolution is possible. Cellular and near cellular resolution is demonstrated in porcine cornea and the drosophila larva, when computational correction of dispersion and aberrations is used. Due to the excellent correction of the used microscope objective, defocus was the main contribution to the aberrations. In addition, higher aberrations caused by the sample itself were successfully corrected. Dispersion and aberrations are closely related artifacts in microscopic OCT imaging. Hence they can be corrected in the same way by optimization of the image quality. This way microscopic resolution is easily achieved in OCT imaging of static biological tissues.},
    keywords = {OCM},
    pubstate = {published},
    tppubtype = {inproceedings}
    }
  • Evers, M; Ha, L; Casper, M; Welford, D; Kositratna, G; Birngruber, R and Manstein, D: Assessment of skin lesions produced by focused, tunable, mid-infrared chalcogenide laser radiation. Lasers in Surgery and Medicine 50(4), 2018
    BibTeX Link
    @article{ Evers2018
       author = {Evers, M; Ha, L; Casper, M; Welford, D; Kositratna, G; Birngruber, R and Manstein, D},
       title = {Assessment of skin lesions produced by focused, tunable, mid-infrared chalcogenide laser radiation},
       journal = {Lasers in Surgery and Medicine} {50(4)},
       DOI = {10.1002/lsm.22935},
      
       year = {2018},
       type = {Journal Article}
    }