Publications AG-Miura

DOI Link

2019

  • Klettner, A and Y, Miura: Porcine RPE/Choroidal Explant Cultures. Springer, 2019
    BibTeX Link
    @book2019{Miura2019,
       author = {Klettner, A and Y, Miura},
       title = {Porcine RPE/Choroidal Explant Cultures},
       publisher = {Springer},
       ISBN = {978-1-4939-8669-2},
      URL = {https://doi.org/10.1007/978-1-4939-8669-9_8},
       year = {2019},
       type = {Book}
    }
    

2018

  • Seifert, E. and Tode, J. and Pielen, A. and Theisen-Kunde, D. and Framme, C. and Roider, J. and Miura, Y. and Birngruber, R. and Brinkmann, R.: Selective retina therapy: toward an optically controlled automatic dosing. J Biomed Opt, no. 23, pp. 1-12, 2018
    BibTeX Link Link
    @article{seifert2018,
       author = {Seifert, E. and Tode, J. and Pielen, A. and Theisen-Kunde, D. and Framme, C. and Roider, J. and Miura, Y. and Birngruber, R. and Brinkmann, R.},
       title = {Selective retina therapy: toward an optically controlled automatic dosing},
       journal = {J Biomed Opt},
       volume = {23},
       number = {11},
       pages = {1-12},
       ISSN = {1560-2281 (Electronic)
    1083-3668 (Linking)},
       DOI = {10.1117/1.JBO.23.11.115002},   
    keywords = {algorithm, lasers in medicine, ophthalmology, retinal pigment epithelium, selective retina therapy, selectivity}
       year = {2018},
       type = {Journal Article}
    }
    
  • Kern, K. and Mertineit, C. L. and Brinkmann, R. and Miura, Y.: Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells. Exp Eye Res, no. 170, pp. 117-126, 2018
    BibTeX Link
    @article{Miura2018,
       author = {Kern, K. and Mertineit, C. L. and Brinkmann, R. and Miura, Y.},
       title = {Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells},
       journal = {Exp Eye Res},
       volume = {170},
       pages = {117-126},
       ISSN = {1096-0007 (Electronic)
    0014-4835 (Linking)},
       DOI = {10.1016/j.exer.2018.02.013},
       year = {2018},
       type = {Journal Article}
    }
    

2017

  • Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.: Power-controlled temperature guided retinal laser therapy. J Biomed Opt, no. 22, pp. 1-11, 2017
    BibTeX Link
    @article{Baade2017,
       author = {Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Power-controlled temperature guided retinal laser therapy},
       journal = {J Biomed Opt},
       volume = {22},
       number = {11},
       pages = {1-11},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.22.11.118001},
       year = {2017},
       type = {Journal Article}
    }
    
  • Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.: Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects. J Vis Exp, no. 30, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.},
       title = {Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects},
       journal = {J Vis Exp},
       number = {124},
       ISSN = {1940-087x},
       DOI = {10.3791/54326},
       year = {2017},
       type = {Journal Article},
    volume = {30}
    
  • Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.: ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch`s Membrane and Causes Its Structural Remodeling. Curr Eye Res, no. 3, pp. 1-12, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.},
       title = {ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch's Membrane and Causes Its Structural Remodeling},
       journal = {Curr Eye Res},
       pages = {1-12},
       ISSN = {0271-3683},
       DOI = {10.1080/02713683.2017.1370118},
       year = {2017},
       type = {Journal Article},
    volume = {3}
    }
    

2016

  • Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.: Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept. Cell Physiol Biochem, no. 38, pp. 737-47, 2016
    BibTeX Link
    @article{Ranjbar2016,
       author = {Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.},
       title = {Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept},
       journal = {Cell Physiol Biochem},
       volume = {38},
       number = {2},
       pages = {737-47},
       note = {1421-9778
    Ranjbar, Mahdy
    Brinkmann, Max Philipp
    Zapf, Dorinja
    Miura, Yoko
    Rudolf, Martin
    Grisanti, Salvatore
    Journal Article
    Switzerland
    Cell Physiol Biochem. 2016;38(2):737-47. doi: 10.1159/000443030. Epub 2016 Feb 15.},
       abstract = {BACKGROUND/AIMS: VEGF-A is induced by oxidative stress, and functions as a survival factor for various cell types, including retinal pigment epithelial (RPE) cells. Anti-vascular endothelial growth factor (VEGF) drugs like aflibercept and bevacizumab have shown to be most effective in treating neovascular age-related macular degeneration (AMD), however uptake of the drugs might lead to interference with cell physiology. Herein, we evaluated the significance of the Fc receptor (FcR) within this context and moreover explored the impact of VEGF inhibition under normal conditions as well as under oxidative stress, in terms of potential adverse effects. METHODS: ARPE-19 (human RPE) cells were treated with aflibercept and bevacizumab in presence or absence of H2O2 as oxidative stress stimulus. After 24h cells were evaluated for drug uptake, VEGF-A expression and secretion, levels of intracellular reactive oxygen species (ROS) as well as cell proliferation. Experiments were repeated with cells being pre-incubated with an FcR inhibitor prior to drug application. RESULTS: Both drugs inhibited extracellular levels of VEGF-A and were taken up into the RPE, resulting in significantly reduced intracellular levels of VEGF-A. When oxidative stress was applied, intracellular ROS levels in cells treated with both drugs rose, and cell proliferation was reduced. Prior incubation with the FcR inhibitor lessened the uptake of bevacizumab, but not aflibercept into RPE cells, and simultaneously enhanced cell survival under oxidative stress conditions. CONCLUSIONS: Our results indicate that uptake and accumulation of aflibercept and bevacizumab within RPE cells affect the intracellular VEGF-A metabolism negatively, leading to a biologically relevant reduced cell survival under oxidative stress. The FcR plays a substantial role in the uptake of bevacizumab, but not aflibercept, which allows an enhanced RPE cell survival through FcR blockage in an environment dominated by oxidative stress, as clinically significant for various inflammatory retinal disorders.},
       ISSN = {1015-8987},
       DOI = {10.1159/000443030},
       year = {2016},
       type = {Journal Article}
    }
    

2014

  • Iwami, H. and Pruessner, J. and Shiraki, K. and Brinkmann, R. and Miura, Y.: Protective effect of a laser-induced sub-lethal temperature rise on RPE cells from oxidative stress. Exp Eye Res, no. 124c, pp. 37-47, 2014
    BibTeX Link
    @article{Iwami2014,
       author = {Iwami, H. and Pruessner, J. and Shiraki, K. and Brinkmann, R. and Miura, Y.},
       title = {Protective effect of a laser-induced sub-lethal temperature rise on RPE cells from oxidative stress},
       journal = {Exp Eye Res},
       volume = {124c},
       pages = {37-47},
       note = {1096-0007
    Iwami, Hisashi
    Pruessner, Joachim
    Shiraki, Kunihiko
    Brinkmann, Ralf
    Miura, Yoko
    Journal article
    Exp Eye Res. 2014 May 5;124C:37-47. doi: 10.1016/j.exer.2014.04.014.},
       abstract = {Recently introduced new technologies that enable temperature-controlled laser irradiation on the RPE allowed us to investigate temperature-resolved RPE cell responses. In this study we aimed primarily to establish an experimental setup that can realize laser irradiation on RPE cell culture with the similar temperature distribution as in the clinical application, with a precise time/temperature history. With this setup, we conducted investigations to elucidate the temperature-dependent RPE cell biochemical responses and the effect of transient hyperthermia on the responses of RPE cells to the secondary-exposed oxidative stress. Porcine RPE cells cultivated in a culture dish (inner diameter = 30 mm) with culture medium were used, on which laser radiation (lambda = 1940 nm, spot diameter = 30 mm) over 10 s was applied as a heat source. The irradiation provides a radially decreasing temperature profile which is close to a Gaussian shape with the highest temperature in the center. Power setting for irradiation was determined such that the peak temperature (Tmax) in the center of the laser spot at the cells reaches from 40 degrees C to 58 degrees C (40, 43, 46, 50, 58 degrees C). Cell viability was investigated with ethidium homodimer III staining at the time points of 3 and 24 h following laser irradiation. Twenty four hours after laser irradiation the cells were exposed to hydrogen peroxide (H2O2) for 5 h, followed by the measurement of intracellular glutathione, intracellular 4-hydroxynonenal (HNE) protein adducts, and secreted vascular endothelial growth factor (VEGF). The mean temperature threshold for RPE cell death after 3 h was found to be around 52 degrees C, and for 24 h around 50 degrees C with the current irradiation setting. A sub-lethal preconditioning on Tmax = 43 degrees C significantly induced the reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, and decreased H2O2-induced increase of intracellular 4-HNE protein adducts. Although sub-lethal hyperthermia (Tmax = 40 degrees C, 43 degrees C, and 46 degrees C) caused a slight increase of VEGF secretion in 6 h directly following irradiation, secondary exposed H2O2-induced VEGF secretion was significantly reduced in the sub-lethally preheated groups, where the largest effect was seen following the irradiation with Tmax = 43 degrees C. In summary, the current results suggest that sub-lethal thermal laser irradiation on the RPE at Tmax = 43 degrees C for 10 s enhances cell defense system against oxidative stress, with increasing the GSH/GSSG ratio. Together with the results that the decreased amount of H2O2-induced 4-HNE in sub-lethally preheated RPE cells was accompanied by the lower secretion of VEGF, it is also strongly suggested that the sub-lethal hyperthermia may modify RPE cell functionality to protect RPE cells from oxidative stress and associated functional decrease, which are considered to play a significant role in the pathogenesis of age-related macular degeneration and other chorioretinal degenerative diseases.},
       ISSN = {0014-4835},
       DOI = {10.1016/j.exer.2014.04.014},
       year = {2014},
       type = {Journal Article}
    }
    
  • Rudolf, M. and Mohi, A. and Dettbarn, M. C. and Miura, Y. and Aherrahrou, Z. and Ranjbar, M. and Mutus, B. and Knobloch, J. K.: Detection of esterified cholesterol in murine Bruch`s membrane wholemounts with a perfringolysin O-based cholesterol marker. Invest Ophthalmol Vis Sci, no. 55, pp. 4759-67, 2014
    BibTeX Link
    @article{Rudolf2014,
       author = {Rudolf, M. and Mohi, A. and Dettbarn, M. C. and Miura, Y. and Aherrahrou, Z. and Ranjbar, M. and Mutus, B. and Knobloch, J. K.},
       title = {Detection of esterified cholesterol in murine Bruch's membrane wholemounts with a perfringolysin O-based cholesterol marker},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {55},
       number = {8},
       pages = {4759-67},
       note = {1552-5783
    Rudolf, Martin
    Mohi, Armin
    Dettbarn, Marie C
    Miura, Yoko
    Aherrahrou, Zouhair
    Ranjbar, Mahdy
    Mutus, Bulent
    Knobloch, Johannes K M
    Journal Article
    Research Support, Non-U.S. Gov't
    United States
    Invest Ophthalmol Vis Sci. 2014 Jul 1;55(8):4759-67. doi: 10.1167/iovs.14-14311.},
       abstract = {PURPOSE: To investigate the effects of Bruch's membrane (BrM) neutral lipid deposition in mouse models and its significance to aging and age-related macular degeneration, it is essential to reliably detect small quantities of neutral lipids including esterified cholesterol (EC). In chorioretinal sections and BrM wholemounts, we tested a novel fluorescent cholesterol marker based on the bacterial toxin perfringolysin O (PFO) and compared results with those obtained with the classic cholesterol dye filipin. METHODS: An engineered plasmid containing the specific cholesterol binding domain (D4) of PFO fused to green fluorescent protein (GFP) was expressed in cultured E. coli, isolated, purified, and concentrated. A total of 150 BrM-choroid wholemounts and chorioretinal sections of 11- to 13-month-old ApoE(null) mice were prepared and stained with PFO/D4-GFP or filipin for EC. Samples were examined by epifluorescence microscopy. RESULTS: The fluorescence intensity of PFO/D4-GFP was strong, stable, and, if small quantities of EC were present, superior to filipin. In all specimens, we could sharply locate the PFO/D4-GFP signal to BrM. A semiquantitative evaluation of BrM lipid deposition is possible by measuring PFO/D4-GFP fluorescence intensity. CONCLUSIONS: The use of PFO/D4-GFP allowed a robust and direct detection of EC in aged murine BrM. In wholemount samples, its strong and stable fluorescence facilitated a semiquantitative evaluation of BrM-EC content over a large area. The patterns of EC deposition in murine BrM wholemounts are comparable with findings in human BrM wholemounts. Perfringolysin O/D4-GFP could be an important tool for investigating the effects of BrM lipid deposition in mouse models.},
       keywords = {Aging/*metabolism/pathology
    Animals
    Bacterial Toxins
    Bruch Membrane/*metabolism/ultrastructure
    Cells, Cultured
    Cholesterol Esters/*metabolism
    Clostridium perfringens
    Disease Models, Animal
    Feasibility Studies
    Female
    Hemolysin Proteins/*diagnostic use
    Humans
    Macular Degeneration/*diagnosis/metabolism
    Mice
    Mice, Inbred C57BL
    Microscopy, Electron, Transmission},
       ISSN = {0146-0404},
       DOI = {10.1167/iovs.14-14311},
       year = {2014},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Caliebe, A. and Portz, L. and Saeger, M. and Miura, Y. and Schlott, K. and Brinkmann, R. and Roider, J.: Comprehensive detection, grading, and growth behavior evaluation of subthreshold and low intensity photocoagulation lesions by optical coherence tomographic and infrared image analysis. Biomed Res Int, no. 2014, pp. 492679, 2014
    BibTeX Link
    @article{Koinzer2014,
       author = {Koinzer, S. and Caliebe, A. and Portz, L. and Saeger, M. and Miura, Y. and Schlott, K. and Brinkmann, R. and Roider, J.},
       title = {Comprehensive detection, grading, and growth behavior evaluation of subthreshold and low intensity photocoagulation lesions by optical coherence tomographic and infrared image analysis},
       journal = {Biomed Res Int},
       volume = {2014},
       pages = {492679},
       note = {2314-6141
    Koinzer, Stefan
    Caliebe, Amke
    Portz, Lea
    Saeger, Mark
    Miura, Yoko
    Schlott, Kerstin
    Brinkmann, Ralf
    Roider, Johann
    Journal Article
    Research Support, Non-U.S. Gov't
    United States
    Biomed Res Int. 2014;2014:492679. doi: 10.1155/2014/492679. Epub 2014 May 12.},
       abstract = {PURPOSE: To correlate the long-term clinical effect of photocoagulation lesions after 6 months, as measured by their retinal damage size, to exposure parameters. We used optical coherence tomographic (OCT)-based lesion classes in order to detect and assess clinically invisible and mild lesions. METHODS: In this prospective study, 488 photocoagulation lesions were imaged in 20 patients. We varied irradiation diameters (100/300 microm), exposure-times (20-200 ms), and power. Intensities were classified in OCT images after one hour, and we evaluated OCT and infrared (IR) images over six months after exposure. RESULTS: For six consecutive OCT-based lesion classes, the following parameters increased with the class: ophthalmoscopic, OCT and IR visibility rate, fundus and OCT diameter, and IR area, but not irradiation power. OCT diameters correlated with exposure-time, irradiation diameter, and OCT class. OCT classes discriminated the largest bandwidth of OCT diameters. CONCLUSION: OCT classes represent objective and valid endpoints of photocoagulation intensity even for "subthreshold" intensities. They are suitable to calculate the treated retinal area. As the area is critical for treatment efficacy, OCT classes are useful to define treatment intensity, calculate necessary lesion numbers, and universally categorize lesions in clinical studies.},
       DOI = {10.1155/2014/492679},
       url = {http://dx.doi.org/10.1155/2014/492679},
       year = {2014},
       type = {Journal Article}
    }
    

2013

  • Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.: Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress. Invest Ophthalmol Vis Sci, 2013
    BibTeX Link
    @article{Miura2013,
       author = {Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.},
       title = {Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress},
       journal = {Invest Ophthalmol Vis Sci},
       note = {Miura, Yoko
    Huettmann, Gereon
    Orzekowsky-Schroeder, Regina
    Steven, Philipp
    Szaszak, Marta
    Koop, Norbert
    Brinkmann, Ralf
    ENG
    2013/04/06 06:00
    Invest Ophthalmol Vis Sci. 2013 Apr 4. pii: iovs.13-11808v1. doi: 10.1167/iovs.13-11808.},
       abstract = {PURPOSE: The aim of this study was to investigate the autofluorescence (AF) of the RPE with two-photon microscopy (TPM) and fluorescence lifetime imaging (FLIM) under normal and oxidative stress conditions. METHODS: Porcine RPE-choroid explants were used for investigation. The RPE-choroid tissue was preserved in a perfusion organ culture system. Oxidative stress was induced by laser photocoagulation with frequency-doubled Nd:YAG laser (532 nm) and by exposure to different concentrations (0, 1, 10 mM) of ferrous sulfate (FeSO4) for 1 hr. At indicated time points after exposure, the tissue was examined with TPM and FLIM. Intracellular reactive oxygen species around the photocoagulation lesion were detected with chloromethyl-2'7'-dichlorofluorescein diacetate (CM-H2DCFDA). Melanosomes were isolated from RPE cells and its fluorescence properties were investigated under normal and oxidized conditions. RESULTS: Under normal condition, AF in RPE cells with TPM is mostly originated from melanosomes, which has a very short fluorescence lifetime (FLT) (mean=117 ps). Under oxidative stress induced by laser irradiation and FeSO4 exposure, bright granular AF appears inside and around RPE cells, whose FLT is significantly longer (mean=1388 ps) than the FLT of the melanosome-AF. Excitation and emission peaks are found at 710-750 nm and 450-500 nm, respectively. Oxidative stress increases the fluorescence intensity of the melanosomes but does not change their FLT. CONCLUSION: TPM reveals acute oxidative stress-induced bright AF granules inside and around RPE cells which can be clearly discriminated from melanosomes by FLIM. TPM combined with FLIM is a useful tool of live-cell analysis to investigate functional alterations of the RPE.},
       year = {2013}
    }
    

2012

  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX
    @article{Miura2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    eng
    England
    2012/03/06 06:00
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       year = {2012}
    }
    
  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX Link
    @article{Treumer2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {1096-0007
    Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    Journal Article
    England
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       ISSN = {0014-4835},
       DOI = {10.1016/j.exer.2012.02.011},
       year = {2012},
       type = {Journal Article}
    }
    
  • Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.: Temperature-controlled retinal photocoagulation - a step toward automated laser treatment. Invest Ophthalmol Vis Sci, no. 53, pp. 3605-14, 2012
    BibTeX
    @article{Koinzer2012,
       author = {Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.},
       title = {Temperature-controlled retinal photocoagulation - a step toward automated laser treatment},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {53},
       number = {7},
       pages = {3605-14},
       note = {Using Smart Source Parsing
    Jun 14; Print 2012 Jul},
       abstract = {Purpose. Retinal laser photocoagulation carries the risk of overtreatment due to effect variation of identically applied lesions. The degree of coagulation depends on the induced temperature increase and on exposure time. We introduce temperature controlled photocoagulation (TCP), which uses optoacoustics to determine individually exposure times necessary to create reproducible lesions. Methods. Optoacoustic temperature measurement relies on pressure waves that are excited in the retinal tissue by repetitive low-energy laser pulses. Signal amplitudes correlate with tissue temperature and are detected by a transducer in the laser contact lens. We used a continuous wave (CW) photocoagulator for treatment irradiation and superimposed probe laser pulses for simultaneous temperature measurement. Optoacoustic data of 1500 lesions (rabbit) were evaluated to develop an algorithm that controls exposure times automatically in TCP. Lesion diameters of 156 TCP lesions were compared to 156 non-controlled lesions. Histology was performed after 1 hour, and 1 and 4 weeks. Results. TCP resulted in exposure times from 4 to 800 ms depending on laser power chosen. Ophthalmoscopic and histologic lesion diameters were independent of power between 14 and 200 mW. TCP lesions barely were visible with a mean diameter equal to the treatment beam (130 mum). In contrast, standard lesion diameters increased linearly and statistically significantly with power. Histology confirmed sparing of the ganglion and nerve fiber layers in TCP. Conclusions. TCP facilitates uniform retinal lesions over a wide power range. In a clinical setting, it should generate soft and reproducible lesions independently of local tissue variation and improve safety, particularly at short exposure times.},
       year = {2012}
    }

2010

  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {Using Smart Source Parsing
    Epub 2009 Oct 29},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       year = {2010}
    }
  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX Link
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {1423-0259
    Miura, Yoko
    Klettner, Alexa
    Noelle, Bernhard
    Hasselbach, Heike
    Roider, Johann
    Journal Article
    Switzerland
    Ophthalmic Res. 2010;43(3):122-33. doi: 10.1159/000252979. Epub 2009 Oct 29.},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       keywords = {Animals
    *Apoptosis
    Bruch Membrane/pathology
    Cell Survival
    Choroid/metabolism/*pathology/surgery
    Collagen Type IV/metabolism
    Enzyme-Linked Immunosorbent Assay
    Fluoresceins/metabolism
    Immunoenzyme Techniques
    In Situ Nick-End Labeling
    Laser Therapy
    Membrane Proteins/metabolism
    Occludin
    Organ Culture Techniques
    Retinal Pigment Epithelium/metabolism/*pathology/surgery
    Swine
    Time Factors
    Vascular Endothelial Growth Factor A/metabolism
    Wound Healing
    von Willebrand Factor/metabolism},
       ISSN = {0030-3747},
       DOI = {10.1159/000252979},
       year = {2010},
       type = {Journal Article}
    }
    

2009

  • Miura, Y. and Roider, J.: Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells. Graefes Arch Clin Exp Ophthalmol, no. 247, pp. 641-9, 2009
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Roider, J.},
       title = {Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {247},
       number = {5},
       pages = {641-9},
       note = {Using Smart Source Parsing
    May; Epub 2009 Feb 3},
       abstract = {PURPOSE: Oxidative stress is known to disrupt the integrity of retinal pigment epithelium (RPE) tight junctions. The goal of this study is to evaluate the effect of triamcinolone acetonide (TA) on the junctional integrity of RPE under oxidative stress and to identify the underlying mechanisms. METHODS: Second passage porcine RPE cells were cultured on 6-well membrane inserts until 4 weeks after reaching confluence. Cells were incubated with TA (10(-5) M) for 30 min. FITC-containing medium was added to the upper chamber (cell's apical side). The cells were then challenged with 1 mM Hydrogen Peroxide (H(2)O(2)). After 5 h, the fluorescence intensity of the medium from lower chamber (cell's basolateral side) was measured using a fluorescence spectrofluorophotometer. This transepithelial flux of FITC-dextran was measured until the 21st day. The immunolocalization of occludin and F-actin was examined with fluorescence microscope. Reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio was determined by a colorimetric assay kit. RESULTS: Non-lethal oxidative stress by H(2)O(2) increased transepithelial flux of FITC-dextran significantly. TA inhibited this increase and preserved the lower flux through the whole experimental period. This permeability change by H(2)O(2) was reversible and recovered to the normal level within 3 weeks. In immunohistological study, H(2)O(2) reduced linear occludin staining at the cell border and increased actin stress fibers. TA prevented H(2)O(2)-induced disruption of junctional assembly of occludin and F-actin. Glutathione assay demonstrated that intracellular GSH/GSSG ratio decreased significantly with H(2)O(2), while TA preserved this ratio by up-regulating GSH synthesis. CONCLUSIONS: TA has a protective effect against oxidative stress-induced disruption of RPE tight junction by preserving cellular redox state.},
       year = {2009}
    }