Dr. rer. nat. Jan Philip Kolb

Postdoc

MPM-Histo




2023

Philipp Lamminger, Hubertus Hakert, Simon Lotz, Jan Philip Kolb, Tonio Kutscher, Sebastian Karpf, and Robert Huber,
Four-Wave Mixing Fast Wavelength Sweeping FDML Laser with kW Peak Power at 900 nm and 1300 nm, in 2023 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , 072023. pp. 1.
DOI:10.1109/CLEO/Europe-EQEC57999.2023.10232141
Bibtex: BibTeX
@INPROCEEDINGS{10232141,
  author={Lamminger, Philipp and Hakert, Hubertus and Lotz, Simon and Kolb, Jan Philip and Kutscher, Tonio and Karpf, Sebastian and Huber, Robert},
  booktitle={2023 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)}, 
  title={Four-Wave Mixing Fast Wavelength Sweeping FDML Laser with kW Peak Power at 900 nm and 1300 nm}, 
  year={2023},
  volume={},
  number={},
  pages={1-1},
  doi={10.1109/CLEO/Europe-EQEC57999.2023.10232141}}
Philipp Lamminger, Hubertus Hakert, Simon Lotz, Jan Philip Kolb, Tonio Kutscher, Sebastian Karpf, and Robert Huber,
Four-wave mixing seeded by a rapid wavelength-sweeping FDML laser for nonlinear imaging at 900 nm and 1300 nm, Opt. Lett. , vol. 48, no. 14, pp. 3713-3716, 07 2023. Optica Publishing Group.
DOI:10.1364/OL.488181
Bibtex: BibTeX
@article{Lamminger:23,
author = {Philipp Lamminger and Hubertus Hakert and Simon Lotz and Jan Philip Kolb and Tonio Kutscher and Sebastian Karpf and Robert Huber},
journal = {Opt. Lett.},
keywords = {Green fluorescent protein; Laser beam combining; Laser crystals; Laser imaging; Optical amplifiers; Photonic crystal lasers},
number = {14},
pages = {3713--3716},
publisher = {Optica Publishing Group},
title = {Four-wave mixing seeded by a rapid wavelength-sweeping FDML laser for nonlinear imaging at 900 nm and 1300 nm},
volume = {48},
month = {Jul},
year = {2023},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-48-14-3713},
doi = {10.1364/OL.488181},
abstract = {Four-wave mixing (FWM) enables the generation and amplification of light in spectral regions where suitable fiber gain media are unavailable. The 1300 nm and 900 nm regions are of especially high interest for time-encoded (TICO) stimulated Raman scattering microscopy and spectro-temporal laser imaging by diffracted excitation (SLIDE) two-photon microscopy. We present a new, to the best of our knowledge, FWM setup where we shift the power of a home-built fully fiber-based master oscillator power amplifier (MOPA) at 1064 nm to the 1300-nm region of a rapidly wavelength-sweeping Fourier domain mode-locked (FDML) laser in a photonic crystal fiber (PCF) creating pulses in the 900-nm region. The resulting 900-nm light can be wavelength swept over 54 nm and has up to 2.5 kW (0.2 {\textmu}J) peak power and a narrow instantaneous spectral linewidth of 70 pm. The arbitrary pulse patterns of the MOPA and the fast wavelength tuning of the FDML laser (419 kHz) allow it to rapidly tune the FWM light enabling new and faster TICO-Raman microscopy, SLIDE imaging, and other applications.},
}
Philipp Lamminger, Hubertus Hakert, Simon Lotz, Jan Philip Kolb, Tonio Kutscher, Sebastian Karpf, and Robert Huber,
900 nm swept source FDML laser with kW peak power, in Fiber Lasers XX: Technology and Systems , V. R. Supradeepa, Eds. SPIE, 032023. pp. 124001I.
DOI:10.1117/12.2649663
Bibtex: BibTeX
@inproceedings{10.1117/12.2649663,
author = {Philipp Lamminger and Hubertus Hakert and Simon Lotz and Jan Philip Kolb and Tonio Kutscher and Sebastian Karpf and Robert Huber},
title = {{900 nm swept source FDML laser with kW peak power}},
volume = {12400},
booktitle = {Fiber Lasers XX: Technology and Systems},
editor = {V.  R. Supradeepa},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {124001I},
abstract = {A wavelength agile 900 nm 2.5 kW peak power fiber laser is created by four-wave mixing (FWM) in a photonic crystal fiber (PCF), while amplifying a 1300 nm Fourier-domain mode-locked (FDML) laser. The FWM process is pumped by a home-built 1064 nm master oscillator power amplifier (MOPA) laser and seeded by a home-built 1300 nm FDML laser, generating high power pulses at wavelengths, where amplification by active fiber media is difficult. The 900 nm pulses have a spectral linewidth of 70 pm, are tunable over 54 nm and have electronic pulse-to-pulse tuning capability. These pulses can be used for nonlinear imaging like two-photon or coherent anti-Stokes Raman microscopy (CARS) microscopy including spectro-temporal laser imaging by diffracted excitation (SLIDE) and time-encoded (Tico) stimulated Raman microscopy.},
keywords = {Fourier domain mode locking,  FDML, Raman, two photon microscopy, SLIDE, 900 nm, fiber laser, photonic crystal fiber, swept source},
year = {2023},
doi = {10.1117/12.2649663},
URL = {https://doi.org/10.1117/12.2649663}
}
Matthias Strauch, Jan Philip Kolb, Christian Rose, Nadine Merg, Jennifer Hundt, Christiane Kümpers, Sven Perner, Sebastian Karpf, and Robert Huber,
Accelerating intraoperative tumor histology with sectioning-free multiphoton microscopy, European Journal of Surgical Oncology , vol. 49, no. 2, pp. e210, 02 2023.
DOI:https://doi.org/10.1016/j.ejso.2022.11.575
File: S0748798322013245
Bibtex: BibTeX
@article{STRAUCH2023e210,
title = {Accelerating intraoperative tumor histology with sectioning-free multiphoton microscopy},
journal = {European Journal of Surgical Oncology},
volume = {49},
number = {2},
pages = {e210},
year = {2023},
issn = {0748-7983},
doi = {https://doi.org/10.1016/j.ejso.2022.11.575},
url = {https://www.sciencedirect.com/science/article/pii/S0748798322013245},
author = {Matthias Strauch and Jan Philip Kolb and Christian Rose and Nadine Merg and Jennifer Hundt and Christiane Kümpers and Sven Perner and Sebastian Karpf and Robert Huber}
}

2022

Christin Grill, Torben Blömker, Mark Schmidt, Dominic Kastner, Tom Pfeiffer, Jan Philip Kolb, Wolfgang Draxinger, Sebastian Karpf, Christian Jirauschek, and Robert Huber,
Towards phase-stabilized Fourier domain mode-locked frequency combs, Communications Physics , vol. 5, no. 1, 08 2022. Springer Science and Business Media LLC.
DOI:10.1038/s42005-022-00960-w
Bibtex: BibTeX
@article{Grill2022,
  doi = {10.1038/s42005-022-00960-w},
  year = {2022},
  publisher = {Springer Science and Business Media {LLC}},
  volume = {{5}},
  number = {{1}},
  author = {C. Grill, T. Bl\"{o}mker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber},
  title = {Towards phase-stabilized Fourier domain mode-locked frequency combs},
  journal = {{Communications Physics}},
keywords={AG-Huber_FDML, FDML, Fourier domain mode locking, phase, frequency comb, coherence, beating}
}
Madita Göb, Tom Pfeiffer, Wolfgang Draxinger, Simon Lotz, Jan Philip Kolb, and Robert Huber,
Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence, Biomed. Opt. Express , vol. 13, no. 2, pp. 713--727, 02 2022. OSA.
DOI:10.1364/BOE.448353
Bibtex: BibTeX
@article{Gob:22,
author = {Madita G\"{o}b and Tom Pfeiffer and Wolfgang Draxinger and Simon Lotz and Jan Philip Kolb and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {High speed imaging; Image processing; Image quality; In vivo imaging; Range imaging; Vertical cavity surface emitting lasers},
number = {2},
pages = {713--727},
publisher = {Optica Publishing Group},
title = {Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence},
volume = {13},
month = {Feb},
year = {2022},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-13-2-713},
doi = {10.1364/BOE.448353},
abstract = {We present continuous three-dimensional spectral zooming in live 4D-OCT using a home-built FDML based OCT system with 3.28 MHz A-scan rate. Improved coherence characteristics of the FDML laser allow for imaging ranges up to 10 cm. For the axial spectral zoom feature, we switch between high resolution and long imaging range by adjusting the sweep range of our laser. We present a new imaging setup allowing for synchronized adjustments of the imaging range and lateral field of view during live OCT imaging. For this, a novel inline recalibration algorithm was implemented that enables numerical k-linearization of the raw OCT fringes for every frame instead of every volume. This is realized by acquiring recalibration data within the dead time of the raster scan at the turning points of the fast axis scanner. We demonstrate in vivo OCT images of fingers and hands at different resolution modes and show real three-dimensional zooming during live 4D-OCT. A three-dimensional spectral zooming feature for live 4D-OCT is expected to be a useful tool for a wide range of biomedical, scientific and research applications, especially in OCT guided surgery.},
}

2021

Madita Göb, Sazgar Burhan, Wolfgang Draxinger, Jan Philip Kolb, and Robert Huber,
Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm2/sec, in European Conferences on Biomedical Optics 2021 (ECBO) , Optical Society of America, Dec.2021. pp. EW3C.4.
DOI:10.1117/12.2616054
Bibtex: BibTeX
@inproceedings{Gob:21,
author = {Madita G\"{o}b and Sazgar Burhan and Wolfgang Draxinger and Jan Philip Kolb and Robert Huber},
booktitle = {European Conferences on Biomedical Optics 2021 (ECBO)},
journal = {European Conferences on Biomedical Optics 2021 (ECBO)},
keywords = {AG-Huber_OCT;Fourier domain mode locking; Image processing; Image quality; Optical coherence tomography; Temporal resolution; Three dimensional imaging},
pages = {EW3C.4},
publisher = {Optical Society of America},
title = {Towards densely sampled ultra-large area multi-MHz-OCT for in vivo skin measurements beyond 1 cm$^2$/sec},
year = {2021},
url = {http://www.osapublishing.org/abstract.cfm?URI=ECBO-2021-EW3C.4},
abstract = {We demonstrate a 3.3 MHz A-scan rate OCT for rapid scanning of large areas of human skin. The mosaicking performance and different OCT imaging modalities including intervolume speckle contrast are evaluated.},
}
Tuula Peñate Medina, Jan Philip Kolb, Gereon Hüttmann, Robert Huber, Oula Peñate Medina, Naomi Larsen, Arianna Ferrari, Magdalena Rafecas, Mark Ellrichmann, Mariya S. Pravdivtseva, Mariia Anikeeva, Jana Humbert, Marcus Both, Jennifer Hundt, and Jan-Bernd Hövener,
Imaging Inflammation - From Whole Body Imaging to Cellular Resolution, Frontiers in immunology , vol. 12, pp. 692222-692222, 06 2021.
DOI:10.3389/fimmu.2021.692222
Bibtex: BibTeX
@article{PenateMedina2021,
   author = {Peñate Medina, T;Kolb, J P;Hüttmann, G;Huber, R;Peñate Medina, O;Ha, L;Ulloa, P;Larsen, N;Ferrari, A;Rafecas, M;Ellrichmann, M;Pravdivtseva, M S.;Anikeeva, M;Humbert, J;Both, M;Hundt, J E. and Hövener, J-B},
   title = {Imaging Inflammation - From Whole Body Imaging to Cellular Resolution},
   journal = {Frontiers in immunology},
keywords = {AG-Huber, MRI, PET, SPECT, optical imaging, Optical coherence tomography (OCT), precision medicine, Two-Photon microscopy (TPM), hyperpolarization},
   volume = {12},
   pages = {692222-692222},
   ISSN = {1664-3224},
   DOI = {10.3389/fimmu.2021.692222},
   url = {https://pubmed.ncbi.nlm.nih.gov/34248987
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264453/},
   year = {2021},
   type = {Journal Article}
}
Christin Grill, Simon Lotz, Torben Blömker, Mark Schmidt, Wolfgang Draxinger, Jan Philip Kolb, Christian Jirauschek, and Robert Huber,
Superposition of two independent FDML lasers, in 2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , 062021. pp. 1-1.
DOI:10.1109/CLEO/Europe-EQEC52157.2021.9542126
Bibtex: BibTeX
@INPROCEEDINGS{9542126,
  author={Grill, Christin and Lotz, Simon and Blömker, Torben and Schmidt, Mark and Draxinger, Wolfgang and Kolb, Jan Philip and Jirauschek, Christian and Huber, Robert},
  booktitle={2021 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)}, 
  title={Superposition of two independent FDML lasers}, 
  year={2021},
  volume={},
  number={},
  pages={1-1},
  abstract={Fourier domain mode locking (FDML) is a laser operating regime, which was developed in 2005 [1] . The output of this laser is a train of optical wavelength sweeps, equivalent to extremely chirped pulses with an optical bandwidth of up to 25 THz and frequency tuning rates of >10 19 Hz/s. This laser type was developed for optical coherence tomography [2] , but found recently more and more applications like LiDAR [3] , Raman microscopy [4] or two-photon microscopy [5] . The laser’s coherence properties are relevant for a better understanding of the FDML laser itself and its applications. Because of the wide sweep range and high tuning rate, the laser linewidth cannot be measured with an RF spectrometer. Superposition with a narrowband continuous wave laser only yields phase information for small fractions of the sweep [6] . However, beat signal measurements between two independent FDML lasers with equal sweep range and direction can give information about the complete sweep.},
  keywords={},
  doi={10.1109/CLEO/Europe-EQEC52157.2021.9542126},
  ISSN={},
  month={June}
}
Matthias Strauch, Jan Philip Kolb, Wolfgang Draxinger, Ann-Kathrin Popp, Melanie Wacker, Nadine Merg, Jennifer Hundt, Sebastian Karpf, and Robert Huber,
Sectioning-free virtual H&E histology with fiber-based two-photon microscopy, in SPIE BiOS , SPIE, 032021.
DOI:10.1117/12.2578334
Bibtex: BibTeX
@inproceedings{RN5318,
   author = {Strauch, M;Kolb, J P;Draxinger, W;Popp, A-K;Wacker, M;Merg, N;Hundt, J;Karpf, S and Huber, R},
   title = {Sectioning-free virtual H&E histology with fiber-based two-photon microscopy},
   booktitle = {SPIE BiOS},
   publisher = {SPIE},
   volume = {11648},
Year = {2021},
   DOI = {https://doi.org/10.1117/12.2578334},
   url = {https://doi.org/10.1117/12.2578334},
   type = {Conference Proceedings}
}
Christin Grill, Torben Blömker, Mark Schmidt, Dominic Kastner, Tom Pfeiffer, Jan Philip Kolb, Wolfgang Draxinger, Sebastian Karpf, Christian Jirauschek, and Robert Huber,
A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 032021. pp. 242 -- 247.
DOI:10.1117/12.2578293
Bibtex: BibTeX
@inproceedings{Grill2021,
author = {C. Grill, T. Blömker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber},
title = {{A detailed analysis of the coherence and field properties of an FDML laser by time resolved beat signal measurements}},
volume = {11665},
booktitle = {Fiber Lasers XVIII: Technology and Systems},
editor = {Michalis N. Zervas},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {242 -- 247},
keywords = {AG-Huber_FDML, Fourier domain mode locking, FDML laser, laser beating , tunable laser, optical coherence tomography, OCT},
year = {2021},
URL = {hhttps://doi.org/10.1117/12.2578293}
}
Simon Lotz, Christin Grill, Madita Göb, Wolfgang Draxinger, Jan Philip Kolb, and Robert Huber,
Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision, Biomedical Optics Express , vol. 12(5), pp. 2604-2616, 03 2021.
DOI:10.1364/BOE.422898
Bibtex: BibTeX
@article{Lotz2021,
   author = {S. Lotz, C. Grill, M. Göb, W. Draxinger, J.P. Kolb and R. Huber},
   title = {Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision},
   journal = {Biomedical Optics Express},
   volume = {12(5)},
   keywords={AG-Huber_FDML},
   pages = {2604-2616},
   url = {https://doi.org/10.1364/BOE.422898},
   year = {2021},
   type = {Journal Article}
}
Philipp Lamminger, Merle Loop, Julian Klee, Daniel Weng, Jan Philip Kolb, Matthias Strauch, Sebastian Karpf, and Robert Huber,
Combination of two-photon microscopy and optical coherence tomography with fully fiber-based lasers for future endoscopic setups, in Multimodal Biomedical Imaging XVI , SPIE, 032021.
DOI:10.1117/12.2578679
Bibtex: BibTeX
@Conference{Lamminger2021,
  author    = {P. Lamminger, M. Loop, J. Klee, D. Weng, J.P. Kolb, M. Strauch, S. Karpf and R. Huber},
  booktitle = {Multimodal Biomedical Imaging XVI},
  title     = {Combination of two-photon microscopy and optical coherence tomography with fully fiber-based lasers for future endoscopic setups},
  year      = {2021},
  publisher = {SPIE},
  doi       = {10.1117/12.2578679},
  keywords  = {AG-Huber_NL, AG-Huber_OCT},
}
Matthias Strauch, Jan Philip Kolb, Christian Rose, Nadine Merg, Christiane Kümpers, Sven Perner, Jennifer Hundt, Sebastian Karpf, and Robert Huber,
Comparison of Sectioning-free Multiphoton Histology to H&E FFPE imaging, in Virtuelle Pathologietage , 2021.
Bibtex: BibTeX
@Conference{Strauch2021b,
  author    = {M. Strauch, J.P. Kolb, C. Rose, N. Merg, J. Hundt, C. Kümpers, S. Perner, S. Karpf and R. Hubert},
  booktitle = {Virtuelle Pathologietage},
  title     = {Comparison of Sectioning-free Multiphoton Histology to H&E FFPE imaging},
  year      = {2021},
  keywords  = {AG-Huber_NL},
}
Matthias Strauch, Jan Philip Kolb, Christian Rose, Nadine Merg, Jennifer Hundt, Christiane Kümpers, Sven Perner, Sebastian Karpf, and Robert Huber,
Quick sectioning-free H&E imaging of bulk tissue using multiphoton microscopy, in 33rd Congress of the ESP , 2021.
DOI:10.1007/s00428-021-03157-8
Bibtex: BibTeX
@Conference{Strauch2021,
  author    = {M. Strauch, J.P. Kolb, C. Rose, N. Merg, J. Hundt, C. Kümpers, S. Perner, S. Karpf and R. Huber},
  booktitle = {33rd Congress of the ESP},
  title     = {Quick sectioning-free H&E imaging of bulk tissue using multiphoton microscopy},
  year      = {2021},
  keywords  = {AG-Huber_NL},
}
Simon Lotz, Christin Grill, Madita Göb, Wolfgang Draxinger, Jan Philip Kolb, and Robert Huber,
Characterization of the dynamics of an FDML laser during closed-loop cavity length control, in Fiber Lasers XVIII: Technology and Systems , Michalis N. Zervas, Eds. SPIE, 2021. pp. 236 -- 241.
DOI:10.1117/12.2578514
Bibtex: BibTeX
@inproceedings{LotzLASE2021,
author = {S. Lotz, C. Grill, M. Göb, W. Draxinger, J. P. Kolb and R. Huber},
title = {{Characterization of the dynamics of an FDML laser during closed-loop cavity length control}},
volume = {11665},
booktitle = {Fiber Lasers XVIII: Technology and Systems},
editor = {Michalis N. Zervas},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {236 -- 241},
abstract = {In Fourier domain mode locked (FDML) lasers, extremely precise and stable matching of the filter tuning period and light circulation time in the cavity is essential for ultra-low noise operation. During the operation of FDML lasers, the ultra-low noise mode can be lost due to temperature drifts of the already temperature stabilized cavity resulting in increased intensity noise. Until now, the filter frequency is continuously regulated to match the changing light circulation time. However, this causes the filter frequency to constantly change by a few mHz and leads to synchronization issues in cases where a fixed filter frequency is desired. We present an actively cavity length controlled FDML laser and a robust and high precision feedback loop algorithm for maintaining ultra-low noise operation. Instead of adapting the filter frequency, the cavity length is adjusted by a motorized free space beam path to match the fixed filter frequency. The closed-loop system achieves a stability of ~0.18 mHz at a sweep repetition rate of ~418 kHz which corresponds to a ratio of 4×10<sup>-10</sup>. We investigate the coherence properties during the active cavity length adjustments and observe no noise increase compared to fixed cavity length. The cavity length control is fully functional and for the first time, offers the possibility to operate an FDML laser in sweet spot mode at a fixed frequency or phase locked to an external clock. This opens new possibilities for system integration of FDML lasers.},
keywords = {AG-Huber_FDML, FDML, Fourier domain mode locking, laser beating, tunable laser, optical coherence tomography, OCT},
year = {2021},
URL = {hhttps://doi.org/10.1117/12.2578514}
}

2020

Tom Pfeiffer, Madita Göb, Wolfgang Draxinger, Sebastian Karpf, Jan Philip Kolb, and Robert Huber,
Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging, Biomed. Opt. Express , vol. 11, no. 11, pp. 6799--6811, Nov. 2020. OSA.
DOI:10.1364/BOE.402477
Bibtex: BibTeX
@article{Pfeiffer:20,
author = {T. Pfeiffer, M. G\"{o}b, W. Draxinger, S. Karpf, J.P. Kolb and R. Huber},
journal = {Biomed. Opt. Express},
keywords = {AG-Huber_OCT; High speed imaging; Image quality; Optical coherence tomography; Swept lasers; Swept sources; Systems design},
number = {11},
pages = {6799--6811},
publisher = {OSA},
title = {Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging},
volume = {11},
month = {Nov},
year = {2020},
doi = {10.1364/BOE.402477},
abstract = {In order to realize adjustable A-scan rates of fast optical coherence tomography (OCT) systems, we investigate averaging of OCT image data acquired with a MHz-OCT system based on a Fourier Domain Mode Locked (FDML) laser. Increased system sensitivity and image quality can be achieved with the same system at the cost of lower imaging speed. Effectively, the A-scan rate can be reduced in software by a freely selectable factor. We demonstrate a detailed technical layout of the strategies necessary to achieve efficient coherent averaging. Since there are many new challenges specific to coherent averaging in swept source MHz-OCT, we analyze them point by point and describe the appropriate solutions. We prove that coherent averaging is possible at MHz OCT-speed without special interferometer designs or digital phase stabilization. We find, that in our system up to \&\#x223C;100x coherent averaging is possible while achieving a sensitivity increase close to the ideal values. This corresponds to a speed reduction from 3.3 MHz to 33 kHz and a sensitivity gain of 20 dB. We show an imaging comparison between coherent and magnitude averaging of a human finger knuckle joint in vivo with 121\&\#x00A0;dB sensitivity for the coherent case. Further, the benefits of computational downscaling in low sensitivity MHz-OCT systems are analyzed.},
}
Matthias Strauch, Jan Philip Kolb, Daniel Weng, Melanie Wacker, Wolfgang Draxinger, Nadine Merg, Jennifer Hundt, Sebastian Karpf, and Robert Huber,
Two-photon microscopy for sectioning-free virtual H&E imaging, in 104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie , 062020.
Weblink: https://www.pathologie-dgp.de/media/Dgp/user_upload/Verhandlungsband_2020_final__kompr._.pdf
Bibtex: BibTeX
@InProceedings{Strauch2020,
  author    = {M. Strauch, J.P. Kolb, D. Weng, M. Wacker, W. Draxinger, N. Merg, J. Hundt, S. Karpf and R. Huber},
  booktitle = {104. Jahrestagung der Deutschen Gesellschaft fuer Pathologie},
  title     = {Two-photon microscopy for sectioning-free virtual {H&E} imaging},
URL = {https://www.pathologie-dgp.de/media/Dgp/user_upload/Verhandlungsband_2020_final__kompr._.pdf},
  year      = {2020},
  keywords  = {AG-Huber_NL},
}
Matthias Strauch, Jan Philip Kolb, Nadine Merg, Jennifer Hundt, Sebastian Karpf, and Robert Huber,
Evaluation of two-photon fluorescence microscopy for sectioning-free H&E imaging of different tissues, in 32nd Congress of the ESP and XXXIII International Congress of the IAP , 2020.
DOI:10.1007/s00428-020-02938-x
Bibtex: BibTeX
@InProceedings{Strauch2020a,
  author    = {M. Strauch, J.P. Kolb, N. Merg, J. Hundt, S. Karpf and R. Huber},
  booktitle = {32nd Congress of the ESP and XXXIII International Congress of the IAP},
  title     = {Evaluation of two-photon fluorescence microscopy for sectioning-free {H&E} imaging of different tissues},
  year      = {2020},
  keywords  = {AG-Huber_NL},
}

2019

Julian Klee, Jan Philip Kolb, Christin Grill, Wolfgang Draxinger, Tom Pfeiffer, and Robert Huber,
Zero roll-off retinal MHz-OCT using an FDML-laser, in Optical Coherence Imaging Techniques and Imaging in Scattering Media III , SPIE, 072019. pp. 110780S.
DOI:10.1117/12.2527034
File: 12.2527034.short
Bibtex: BibTeX
@inproceedings{10.1117/12.2527034,
author = {Julian Klee and Jan Philip Kolb and Christin Grill and Wolfgang Draxinger and Tom Pfeiffer and Robert Huber},
title = {{Zero roll-off retinal MHz-OCT using an FDML-laser}},
volume = {11078},
booktitle = {Optical Coherence Imaging Techniques and Imaging in Scattering Media III},
editor = {Maciej Wojtkowski and Stephen A. Boppart and Wang-Yuhl Oh},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {110780S},
abstract = {Optical coherence tomography (OCT) applications like ultra-widefield and full eye-length imaging are of high interest for various diagnostic purposes. In swept-source OCT these techniques require a swept light source, which is coherent over the whole imaging depth. We present a zero roll-off 1060 nm Fourier Domain Mode Locked-Laser (FDML-Laser) for retinal OCT imaging at 1.7 MHz A-scan rate and first long-range imaging results with it. Several steps such as improved dispersion compensation and frequency regulation were performed and will be discussed. Besides virtually no loss in OCT signal over the maximum depth range of 4.6 mm and very good dynamic range was observed. Roll-off measurements show no decrease of the point-spread function (PSF), while maintaining a high dynamic range.},
keywords = {optical coherence tomography, OCT, tunable laser, Fourier Domain Mode Locking, FDML, MHz OCT},
year = {2019},
doi = {10.1117/12.2527034},
URL = {https://doi.org/10.1117/12.2527034}
}
Daniel Weng, Hubertus Hakert, Torben Blömker, Jan Philip Kolb, Matthias Strauch, Matthias Eibl, Philipp Lamminger, Sebastian Karpf, and Robert Huber,
Sub-Nanosecond Pulsed Fiber Laser for 532nm Two-Photon Excitation Fluorescence (TPEF) Microscopy of UV Transitions, in 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) , IEEE, 062019. pp. 1-1.
DOI:10.1109/CLEOE-EQEC.2019.8872571
Bibtex: BibTeX
@INPROCEEDINGS{8872571,
  author={Weng, Daniel and Hakert, Hubertus and Blömker, Torben and Kolb, Jan Philip and Strauch, Matthias and Eibl, Matthias and Lamminger, Philipp and Karpf, Sebastian and Huber, Robert},
  booktitle={2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC)}, 
  title={Sub-Nanosecond Pulsed Fiber Laser for 532nm Two-Photon Excitation Fluorescence (TPEF) Microscopy of UV Transitions}, 
  year={2019},
  volume={},
  number={},
  pages={1-1},
  abstract={Summary form only given. Two-photon microscopy is a powerful technique for in vivo imaging, due to its high penetration depth and axial sectioning. Usually excitation wavelengths in the near infrared are used. However, most fluorescence techniques for live cell imaging require labeling with exogenous fluorophores. It has been shown that shorter wavelengths can be used to excite the autofluorescence of endogenous proteins, e.g. tryptophan. Recently we demonstrated a fully fiber-based laser source built around a directly modulated, ytterbium amplified 1064 nm laser diode with sub-nanosecond pulses for two-photon imaging [2]. The overall system enables to capture high-speed fluorescence lifetime imaging (FLIM) with single pulse excitation. Here, we extend the spectral range of this laser source by frequency doubling it to 532nm to achieve two-photon excited fluorescence microscopy (TPM) in the ultraviolett (UV) range to harness endogenous autofluorescence. In this presentation we explore first TPM results of tryptophan to investigate signal levels and fi delity before transitioning to biological tissues. It has been shown that TPM of endogenous tryptophan can be used to visualize immune system activity in vivo. Our laser source could be a cheap, flexible and fiber-based alternative to the OPO-based Ti:Sa Lasers currently employed. The basic concept of our design is to shift the wavelength of the pulsed fiber-based master oscillator power amplifier (MOPA) by second-harmonic generation (SHG) using phase-matching in a KTP crystal. This generates a coherent output at 532nm at a maximal peak power of 500W. We achieved a maximum conversion efficiency of about 17%. After the SHG module, the 532nm light is coupled into a single-mode fiber and delivered to a home built microscope. A 40x microscope objective is used to excite the sample and epi-collect the fluorescence. The fluorescence is recorded on a UV-enhanced photomultiplier tube (PMT). For a proof of concept measurement, crystalized tryptophan was imaged. Here we show signals of pure tryptophan, with laser parameters of 1MHz repetition rate and 100ps pulse duration. We used spectral bandpass fi lters in order to detect only fluorescence signal, however, from crystalized tryptophan we observed an unexpected short lifetime. We have recently shown that we can shift our laser output from 1064nm to longer wavelengths. By shifting to 1180nm and frequency doubling to 590nm a more efficient fluorescence excitation of tryptophan can be achieved. In the future we aim at in vivo imaging with our setup.},
  keywords={},
  doi={10.1109/CLEOE-EQEC.2019.8872571},
  ISSN={},
  month={June}}
Jan Philip Kolb, Wolfgang Draxinger, Julian Klee, Tom Pfeiffer, Matthias Eibl, Thomas Klein, Wolfgang Wieser, and Robert Huber,
Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates, PLOS ONE , vol. 14, no. 7, pp. e0213144, 03 2019.
DOI:10.1371/journal.pone.0213144
Bibtex: BibTeX
@article{Kolb2019,
   author = {Kolb, J P;Draxinger, W;Klee, J;Pfeiffer, T;Eibl, M;Klein, T;Wieser, W and Huber, R},
   title = {Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates},
   journal = {J pone},
 keywords = {AG-Huber_OCT},
   url = {https://doi.org/10.1371/journal.pone.0213144},
   pages = {e0213144},
   ISSN = {1932-6203},
   
   year = {2019},
   type = {Journal Article}
}
Jan Philip Kolb, Daniel Weng, Hubertus Hakert, Matthias Eibl, Wolfgang Draxinger, Tobias Meyer-Zedler, Thomas Gottschall, Ralf Brinkmann, Reginald Birngruber, Jürgen Popp, Jens Limpert, Sebastian Karpf, and Robert Huber,
Virtual HE histology by fiber-based picosecond two-photon microscopy, in Multiphoton Microscopy in the Biomedical Sciences XIX , Ammasi Periasamy; Peter T. C. So; Karsten König, Eds. International Society for Optics and Photonics, 022019. pp. 108822F.
DOI:10.1117/12.2507866
Bibtex: BibTeX
@inproceedings{10.1117/12.2507866,
author = {Jan Philip Kolb and Daniel Weng and Hubertus Hakert and Matthias Eibl and Wolfgang Draxinger and Tobias Meyer and Thomas Gottschall and Ralf  Brinkmann and Reginald Birngruber and J{\"u}rgen Popp and Jens Limpert and Sebastian Nino Karpf and Robert Huber},
title = {{Virtual HE histology by fiber-based picosecond two-photon microscopy}},
volume = {10882},
booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX},
editor = {Ammasi Periasamy and Peter T. C. So and Karsten K{\"o}nig},
organization = {International Society for Optics and Photonics},
publisher = {SPIE},
pages = {108822F},
abstract = {Two-Photon Microscopy (TPM) can provide three-dimensional morphological and functional contrast in vivo. Through proper staining, TPM can be utilized to create virtual, HE equivalent images and thus can improve throughput in histology-based applications. We previously reported on a new light source for TPM that employs a compact and robust fiber-amplified, directly modulated laser. This laser is pulse-to-pulse wavelength switchable between 1064 nm, 1122 nm, and 1186 nm with an adjustable pulse duration from 50ps to 5ns and arbitrary repetition rates up to 1MHz at kW-peak powers. Despite the longer pulse duration, it can achieve similar average signal levels compared to fs-setups by lowering the repetition rate to achieve similar cw and peak power levels. The longer pulses lead to a larger number of photons per pulse, which yields single shot fluorescence lifetime measurements (FLIM) by applying a fast 4 GSamples/s digitizer. In the previous setup, the wavelengths were limited to 1064 nm and longer. Here, we use four wave mixing in a non-linear photonic crystal fiber to expand the wavelength range down to 940 nm. This wavelength is highly suitable for imaging green fluorescent proteins in neurosciences and stains such as acridine orange (AO), eosin yellow (EY) and sulforhodamine 101 (SR101) used for histology applications. In a more compact setup, we also show virtual HE histological imaging using a direct 1030 nm fiber MOPA.},
keywords = {Multiphoton Microscopy, Four Wave Mixing, FWM, Histology, Laser, Non Linear Microscopy, Two Photon Microscopy, JenLab Young Investigator Award},
year = {2019},
doi = {10.1117/12.2507866},
URL = {https://doi.org/10.1117/12.2507866}
}
Matthias Strauch, Jan Philip Kolb, Daniel Weng, Melanie Wacker, Wolfgang Draxinger, Sebastian Karpf, and Robert Huber,
Sectioning-Free Virtual H&E Imaging of Tissue Samples with Two-Photon Microscopy, in 31st Congress of the ESP , 2019.
DOI:10.1007/s00428-019-02631-8
Bibtex: BibTeX
@InProceedings{Strauch2019,
  author    = {Strauch, Matthias and Kolb, Jan Philip and Weng, Daniel and Wacker, Melanie and Draxinger, Wolfgang and Karpf, Sebastian and Huber, Robert},
  booktitle = {31st Congress of the ESP},
  title     = {Sectioning-Free Virtual H&E Imaging of Tissue Samples with Two-Photon Microscopy},
  year      = {2019},
  keywords  = {AG-Huber_NL},
}

2018

Josef Maertz, Jan Philip Kolb, Thomas Klein, Kathrin J. Mohler, Matthias Eibl, Wolfgang Wieser, Robert Huber, Siegfried Priglinger, and Armin Wolf,
Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm, Graefes Arch Clin Exp Ophthalmol , vol. 256, no. 2, pp. 289-298, Dec. 2018.
DOI:10.1007/s00417-017-3857-9
Bibtex: BibTeX
@article{Maertz2018,
   author = {Maertz, J; Kolb, J P; Klein, T; Mohler, K J; Eibl, M; Wieser, W; Huber, R; Priglinger, S and Wolf, A},
   title = {Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   number = {2},
   pages = {289-298},
   DOI = {10.1007/s00417-017-3857-9},
   url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032262413&doi=10.1007%2fs00417-017-3857-9&partnerID=40&md5=a46c315f12cf5e633ea0f7e644116eb3},
   year = {2018},
   Keywords= {En face imaging, Optical coherence tomography, Swept-source OCT, Megahertz OCT, 3D rendering, Optic disc, Optic disc pit, Optic disc pit maculopathy, AG-Huber_OCT},
   type = {Journal Article}
}