Publikationen

2018

  • Rudnitzki, F; Feineis, S; Rahmanzadeh, R; Endl, E; Lutz, J; Groll, J and Huettmann, G: iRNA release from gold nanoparticles by nanosecond pulsed irradiation and analysis of the involved temperature increase. Journal of BIOPHOTONICS 11(9), 2018
    BibTeX Link
    @article{Rahmanzadeh2018,
       author = {Rudnitzki, F; Feineis, S; Rahmanzadeh, R; Endl, E; Lutz, J; Groll, J and Huettmann, G},
       title = {siRNA release from gold nanoparticles by nanosecond pulsed
    laser irradiation and analysis of the involved temperature
    increase},
       journal = {Journal of BIOPHOTONICS} {11(9)},
      doi = {10.1002/jbio.201700329},
       
    
    keywords = {cavitation|cell manipulation|controlled release|gold nanoparticle bio-conjugates|laser nanoeffects},
    abstract = {Nanosecond pulsed laser irradiation can trigger a release of nucleic acids from gold nanoparticles, but the involved nanoeffects are not fully understood yet. Here we investigate the release of coumarin labeled siRNA from 15 to 30 nm gold particles after nanosecond pulsed laser irradiation. Temperatures in the particle and near the surface were calculated for the different radiant exposures. Upon irradiation with laser pulses of 4 nanosecond duration release started for both particle sizes at a calculated temperature increase of approximately 500 K. Maximum coumarin release was observed for 15 nm particles after irradiation with radiant exposure of 80 mJ cm−2 and with 32 mJ cm−2 for 30 nm particles. This corresponds to a temperature increase of 815 and 900 K, respectively. Our results show that the molecular release by nanosecond pulsed irradiation is based on a different mechanism compared to continuous or femtosecond irradiation. Local temperatures are considerably higher and it is expected that bubble formation plays a crucial role in release and damage to cellular structures. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim}
       year = {2018},
       type = {Journal Article}
    }
    

2017

  • Yao, C; Rudnitzki, F; Hüttmann, G; Zhang, Zand Rahmanzadeh, R: Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation. International Journal of Nanomedicine Volume 12, pp. 5659-5672, 2017
    BibTeX Link
    @article{Yao2017,
       author = {Yao, C; Rudnitzki, F; Hüttmann, G; Zhang, Zand Rahmanzadeh, R},
       title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
    journal = {International Journal of Nanomedicine} {Volume 12},
      
       pages = {5659-5672},
       DOI = {10.2147/IJN.S140620},
       year = {2017},
       type = {Journal Article}
    }
    

2016

  • Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy. Journal of Biomedical Optics, no. 21, pp. 078001-078001, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {7},
       pages = {078001-078001},
       note = {10.1117/1.JBO.21.7.078001},
       abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
       ISSN = {1083-3668},
       year = {2016},
       type = {Journal Article}
    }
    
  • Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.: Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b. J Cancer Res Clin Oncol, no. 142, pp. 1261-71, 2016
    BibTeX Link
    @article{Maushagen2016,
       author = {Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.},
       title = {Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b},
       journal = {J Cancer Res Clin Oncol},
       volume = {142},
       number = {6},
       pages = {1261-71},
       note = {1432-1335
    Maushagen, Regina
    Reers, Stefan
    Pfannerstill, Ann-Christin
    Hahlbrock, Angelina
    Stauber, Roland
    Rahmanzadeh, Ramtin
    Rades, Dirk
    Pries, Ralph
    Wollenberg, Barbara
    Journal Article
    Germany
    J Cancer Res Clin Oncol. 2016 Jun;142(6):1261-71. doi: 10.1007/s00432-016-2150-3. Epub 2016 Apr 1.},
       abstract = {PURPOSE: Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to beta-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet. METHODS: The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies. RESULTS: Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome. CONCLUSION: Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.},
       keywords = {Apoptosis
    Caspase 9b
    Caspases
    Head and neck cancer
    Paclitaxel},
       ISSN = {0171-5216},
       DOI = {10.1007/s00432-016-2150-3},
       year = {2016},
       type = {Journal Article}
    }
    
    
  • Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.: A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67. Sci Rep, no. 6, pp. 27032, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
       title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
       journal = {Sci Rep},
       volume = {6},
       pages = {27032},
       note = {2045-2322
    Wang, Sijia
    Huttmann, Gereon
    Scholzen, Thomas
    Zhang, Zhenxi
    Vogel, Alfred
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal Article
    England
    Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
       abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
       ISSN = {2045-2322},
       DOI = {10.1038/srep27032},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.: Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67. Mol Pharm, 2015
    BibTeX Link
    @article{Wang2015,
       author = {Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.},
       title = {Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67},
       journal = {Mol Pharm},
       note = {1543-8392
    Wang, Sijia
    Huttmann, Gereon
    Zhang, Zhenxi
    Vogel, Alfred
    Birngruber, Reginald
    Tangutoori, Shifalika
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal article
    Mol Pharm. 2015 Aug 13.},
       abstract = {The selective inhibition of intracellular and nuclear molecules such as Ki-67 holds great promise for the treatment of cancer and other diseases. However, the choice of the target protein and the intracellular delivery of the functional agent remain crucial challenges. Main hurdles are (a) an effective delivery into cells, (b) endosomal escape of the delivered agents, and (c) an effective, externally triggered destruction of cells. Here we show a light-controlled two-step approach for selective cellular delivery and cell elimination of proliferating cells. Three different cell-penetrating nano constructs, including liposomes, conjugates with the nuclear localization sequence (NLS), and conjugates with the cell penetrating peptide Pep-1, delivered the light activatable antibody conjugate TuBB-9-FITC, which targets the proliferation associated protein Ki-67. HeLa cells were treated with the photosensitizer benzoporphyrin monoacid derivative (BPD) and the antibody constructs. In the first optically controlled step, activation of BPD at 690 nm triggered a controlled endosomal escape of the TuBB-9-FITC constructs. In more than 75% of Ki-67 positive, irradiated cells TuBB-9-FITC antibodies relocated within 24 h from cytoplasmic organelles to the cell nucleus and bound to Ki-67. After a second light irradiation at 490 nm, which activated FITC, cell viability decreased to approximately 13%. Our study shows an effective targeting strategy, which uses light-controlled endosomal escape and the light inactivation of Ki-67 for cell elimination. The fact that liposomal or peptide-assisted delivery give similar results leads to the additional conclusion that an effective mechanism for endosomal escape leaves greater variability for the choice of the delivery agent.},
       keywords = {endosomal entrapment
    liposome
    nanotechnology
    nuclear localization sequence (NLS)
    photodynamic therapy},
       ISSN = {1543-8384},
       DOI = {10.1021/acs.molpharmaceut.5b00260},
       year = {2015},
       type = {Journal Article}
    }
    

2012

  • Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon: Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction. Journal of Biomedical Optics, no. 17, pp. 058003, 2012
    BibTeX
    @article{Rudnitzki2012,
       author = {Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon},
       title = {Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {5},
       pages = {058003},
       year = {2012}
    }

2011

  • Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.: The proliferation marker Ki-67 as novel molecular target for PDT. Photodiagnosis and Photodynamic Therapy, no. 8, pp. 129, 2011
    BibTeX Link
    @article{Rahmanzadeh2011,
       author = {Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.},
       title = {The proliferation marker Ki-67 as novel molecular target for PDT},
       journal = {Photodiagnosis and Photodynamic Therapy},
       volume = {8},
       number = {2},
     pages = {129},
       DOI = {10.1016/j.pdpdt.2011.03.025},
       year = {2011},
       type = {Journal Article}
    }
    
  • Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba: Targeted molecular effects through laser-irradiated nanoabsorbers. 2011
    BibTeX Link
    @inproceedings{Hüttmann2011,
       author = {Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba},
       title = {Targeted molecular effects through laser-irradiated nanoabsorbers},
       editor = {Newsrrom, SPIE},
       DOI = {10.1117/2.1201102.003548},
       type = {Conference Proceedings},
       year = { 2011}
    }
    

2010

  • Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba: Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells. no. 7576, pp. 757602, SPIE, 2010
    BibTeX Link
    @inproceedings{Rahmanzadeh,
       author = {Rahmanzadeh, Ramtin and Rai, Prakash and Gerdes, Johannes and Hasan, Tayyaba},
       title = {Targeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells},
       editor = {Samuel, Achilefu and Ramesh, Raghavachari},
       publisher = {SPIE},
       volume = {7576},
       pages = {757602},
    year = {2010},
    doi ={10.1117/12.843850},
    keywords = {Nanotechnology, Ovarian Cancer, Proliferative Index, Photodynamic Therapy,Antibody}
    
    }
  • Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S: Photodynamic therapy to treat ovarian cancer. no. 5, National Cancer Institute (NCI) Alliance for Nanotechnology, 2010
    BibTeX
    @misc{Hasan,
       author = {Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S},
       title = {Photodynamic therapy to treat ovarian cancer},
       publisher = {National Cancer Institute (NCI) Alliance for Nanotechnology},
       volume = {5},
       number = {4},
       year = {2010}
    }
  • Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.: Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer. Cancer Res, no. 70, pp. 9234-42, 2010
    BibTeX
    @article{Rahmanzadeh2010,
       author = {Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.},
       title = {Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer},
       journal = {Cancer Res},
       volume = {70},
       number = {22},
       pages = {9234-42},
       note = {Using Smart Source Parsing
    Nov 15; Epub 2010 Nov 2},
       abstract = {Targeting molecular markers and pathways implicated in cancer cell growth is a promising avenue for developing effective therapies. Although the Ki-67 protein (pKi-67) is a key marker associated with aggressively proliferating cancer cells and poor prognosis, its full potential as a therapeutic target has never before been successfully shown. In this regard, its nuclear localization presents a major hurdle because of the need for intracellular and intranuclear delivery of targeting and therapeutic moieties. Using a liposomally encapsulated construct, we show for the first time the specific delivery of a Ki-67-directed antibody and subsequent light-triggered death in the human ovarian cancer cell line OVCAR-5. Photoimmunoconjugate-encapsulating liposomes (PICEL) were constructed from anti-pKi-67 antibodies conjugated to fluorescein 5(6)-isothiocyanate, as a photoactivatable agent, followed by encapsulation in noncationic liposomes. Nucleolar localization of the PICELs was confirmed by confocal imaging. Photodynamic activation with PICELs specifically killed pKi-67-positive cancer cells both in monolayer and in three-dimensional (3D) cultures of OVCAR-5 cells, with the antibody TuBB-9 targeting a physiologically active form of pKi-67 but not with MIB-1, directed to a different epitope. This is the first demonstration of (a) the exploitation of Ki-67 as a molecular target for therapy and (b) specific delivery of an antibody to the nucleolus in monolayer cancer cells and in an in vitro 3D model system. In view of the ubiquity of pKi-67 in proliferating cells in cancer and the specificity of targeting in 3D multicellular acini, these findings are promising and the approach merits further investigation.},
       year = {2010}
    }
  • Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.: Development and applications of photo-triggered theranostic agents. Adv Drug Deliv Rev, no. 62, pp. 1094-124, 2010
    BibTeX
    @article{Rai,
       author = {Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.},
       title = {Development and applications of photo-triggered theranostic agents},
       journal = {Adv Drug Deliv Rev},
       volume = {62},
       number = {11},
       pages = {1094-124},
       note = {Rai, Prakash
    Mallidi, Srivalleesha
    Zheng, Xiang
    Rahmanzadeh, Ramtin
    Mir, Youssef
    Elrington, Stefan
    Khurshid, Ahmat
    Hasan, Tayyaba
    Nihms238162
    Adv Drug Deliv Rev. 2010 Aug 30;62(11):1094-124. Epub 2010 Sep 19.},
       abstract = {Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.},
       keywords = {Animals
    Anti-Infective Agents/diagnostic use/therapeutic use
    Antineoplastic Agents/diagnostic use/therapeutic use
    Diagnostic Imaging/ methods
    Drug Carriers/diagnostic use/therapeutic use
    Humans
    Infection/ diagnosis/ drug therapy
    Nanoparticles/diagnostic use/therapeutic use
    Neoplasms/ diagnosis/drug therapy/ therapy
    Phototherapy/ methods},
       year = {2010}
    }
    
    
  • Rahmanzadeh, R and Rai, P. and Gerdes, J. and Hasan, T: argeted light-inactivation of the Ki-67 protein using theranostic liposomes leads to death of proliferating cells. Proc.of Spie, no. 7576, 2010
    BibTeX Link Link
    @article{Rahmanzadeh2010,
       author = {Rahmanzadeh, R and Rai, P. and Gerdes, J. and Hasan, T},
       title = {Targeted light-inactivation of the Ki-67 protein using
    theranostic liposomes leads to death of proliferating cells},
       journal = {Proc.of Spie},
       volume = {7576},
    keywords = {Nanotechnology, Ovarian Cancer, Proliferative Index, Photodynamic Therapy,Antibody}
       DOI = {10.1117/12.843850},
       url = {http://www.massgeneral.org/wellman/people/thasan.asp},
       year = { 2010},
       type = {Journal Article}
    }
    

2009

  • Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.: Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection. J Biomed Opt, no. 14, pp. 054034, 2009
    BibTeX
    @article{Yao,
       author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
       title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
       journal = {J Biomed Opt},
       volume = {14},
       pages = {054034},
       note = {Journal article},
       year = {2009}
    }

2008

  • Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.: Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests. in Plant J, no. 54, pp. 938-48, 2008
    BibTeX
    @incollection{Phillips,
       author = {Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.},
       title = {Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests},
       booktitle = {Plant J},
       volume = {54},
       edition = {2008/03/19},
       pages = {938-48},
       note = {Phillips, Jonathan R
    Fischer, Eberhard
    Baron, Miriam
    van den Dries, Niels
    Facchinelli, Fabio
    Kutzer, Michael
    Rahmanzadeh, Ramtin
    Remus, Daniela
    Bartels, Dorothea
    England
    Plant J. 2008 Jun;54(5):938-48. Epub 2008 Mar 13.},
       abstract = {A particular adaptation to survival under limited water availability has been realized in the desiccation-tolerant resurrection plants, which tend to grow in a habitat with seasonal rainfall and long dry periods. One of the best-studied examples is Craterostigma plantagineum. Here we report an unexpected finding: Lindernia brevidens, a close relative of C. plantagineum, exhibits desiccation tolerance, even though it is endemic to the montane rainforests of Tanzania and Kenya, where it never experiences seasonal dry periods. L. brevidens has been found exclusively in two fragments of the ancient Eastern Arc Mountains, which were protected from the devastating Pleistocene droughts by the stable Indian Ocean temperature. Analysis of the microhabitat reveals that L. brevidens is found in the same habitat as hygrophilous plant species, which further indicates that the plant never dries out completely. The objective of this investigation was to address whether C. plantagineum and L. brevidens have desiccation-related pathways in common, or whether L. brevidens has acquired novel pathways. A third, closely related, desiccation-sensitive species, Lindernia subracemosa, has been included for comparison. Mechanisms that confer cellular protection during extreme water loss are well conserved between C. plantagineum and L. brevidens, including the interconversion of 2-octulose to sucrose within the two desiccation-tolerant species. Furthermore, transcriptional control regions of desiccation-related genes belonging to the late embryogenesis abundant (LEA) protein family are also highly conserved. We propose that L. brevidens is a neoendemic species that has retained desiccation tolerance through genome stability, despite tolerance being superfluous to environmental conditions.},
       keywords = {Adaptation, Physiological
    Gene Expression Profiling
    Genome, Plant
    Lamiaceae/genetics/metabolism/ physiology
    Sucrose/metabolism
    Tropical Climate
    Water},
       year = {2008}
    }
  • Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.: Laser-based gene transfection and gene therapy. IEEE Trans Nanobioscience, no. 7, pp. 111-9, 2008
    BibTeX
    @article{Yao,
       author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.},
       title = {Laser-based gene transfection and gene therapy},
       journal = {IEEE Trans Nanobioscience},
       volume = {7},
       number = {2},
       pages = {111-9},
       note = {Yao, C P
    Zhang, Z X
    Rahmanzadeh, R
    Huettmann, G
    Research Support, Non-U.S. Gov't
    Review
    United States
    IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.},
       abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.},
       keywords = {Cell Membrane/ radiation effects
    DNA/ administration & dosage/ pharmacokinetics
    Gene Therapy/ methods
    Lasers
    Transfection/ methods},
       year = {2008}
    }
  • Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.: Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes. no. 13, pp. 031217, 2008
    BibTeX
    @misc{Qu,
       author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.},
       title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes},
       volume = {13},
       number = {3},
       pages = {031217},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.},
       ISBN = {1083-3668 (Print)
    1083-3668 (Linking)},
       year = {2008}
    }

2007

  • Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.: Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif, no. 40, pp. 422-30, 2007
    BibTeX
    @article{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.},
       title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis},
       journal = {Cell Prolif},
       volume = {40},
       number = {3},
       pages = {422-30},
       note = {Rahmanzadeh, R
    Huttmann, G
    Gerdes, J
    Scholzen, T
    England
    Cell Prolif. 2007 Jun;40(3):422-30.},
       abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.},
       keywords = {Antibodies, Antinuclear
    Antibodies, Monoclonal
    Cell Division/physiology
    Cell Nucleolus/physiology
    Fluorescein-5-isothiocyanate
    Fluorescent Dyes
    HeLa Cells
    Humans
    Ki-67 Antigen/*genetics/*metabolism
    Photochemistry
    RNA Polymerase I/metabolism
    RNA, Ribosomal/*biosynthesis},
       year = {2007}
    }

2005

  • Rahmanzadeh, R. and Muller, K. and Fischer, E. and Bartels, D. and Borsch, T.: The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales). no. 7, pp. 67-78, Jan, 2005
    BibTeX
    @misc{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Muller, K. and Fischer, E. and Bartels, D. and Borsch, T.},
       title = {The Linderniaceae and Gratiolaceae are further lineages distinct from the Scrophulariaceae (Lamiales)},
       volume = {7},
       number = {1},
       pages = {67-78},
       month = {Jan},
       note = {Rahmanzadeh, R
    Muller, K
    Fischer, E
    Bartels, D
    Borsch, T
    Research Support, Non-U.S. Gov't
    Germany
    Plant Biol (Stuttg). 2005 Jan;7(1):67-78.},
       abstract = {The Lamiales are one of the largest orders of angiosperms, with about 22,000 species. The Scrophulariaceae, as one of their most important families, has recently been shown to be polyphyletic. As a consequence, this family was re-classified and several groups of former scrophulariaceous genera now belong to different families, such as the Calceolariaceae, Plantaginaceae, or Phrymaceae. In the present study, relationships of the genera Craterostigma, Lindernia and its allies, hitherto classified within the Scrophulariaceae, were analyzed. Sequences of the chloroplast trnK intron and the matK gene (approximately 2.5 kb) were generated for representatives of all major lineages of the Lamiales and the former Scrophulariaceae. Bayesian and parsimony analyses revealed two isolated lineages, one of which consists of Lindernia and its allies, the other of Gratiola and allies. Gratiola was previously assumed to be related to Lindernia and was therefore included here. It is proposed to treat the two clades as separate families, Linderniaceae and Gratiolaceae. For the Linderniaceae, several morphological synapomorphies exist in addition to molecular data, such as conspicuous club-shaped stamen appendages.},
       keywords = {Bayes Theorem
    Genes, Plant
    Introns
    Phenotype
    Phylogeny
    Scrophulariaceae/anatomy & histology/ classification/genetics
    Species Specificity},
       ISBN = {1435-8603 (Print)
    1435-8603 (Linking)},
       year = {2005}
    }
  • Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.: Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles. J Biomed Opt, no. 10, pp. 064012, 2005
    BibTeX
    @article{Yao,
       author = {Yao, C. and Rahmanzadeh, R. and Endl, E. and Zhang, Z. and Gerdes, J. and Huttmann, G.},
       title = {Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles},
       journal = {J Biomed Opt},
       volume = {10},
       number = {6},
       pages = {064012},
       note = {Yao, Cuiping
    Rahmanzadeh, Ramtin
    Endl, Elmar
    Zhang, Zhenxi
    Gerdes, Johannes
    Huttmann, Gereon
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2005 Nov-Dec;10(6):064012.},
       abstract = {Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50 nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532 nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin's disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.},
       keywords = {Biopolymers/pharmacokinetics
    Cell Line, Tumor
    Cell Membrane Permeability/ physiology/ radiation effects
    Drug Delivery Systems/ methods
    Fluoresceins/ pharmacokinetics
    Humans
    Lasers
    Lymphoma/ metabolism
    Nanostructures},
       year = {2005}
    }