Publikationen 2016 - 2017

2017

  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert: Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection. in Proc. SPIE, no. 10069, pp. 100691F-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert},
       title = {Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection},
    booktitle = {Proc. SPIE },
       volume = {10069},
       pages = {100691F-6},
       doi= {10.1117/12.2250831},
      year={2017},
       type = {Conference Proceedings}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Amenta, Gaetano and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and Soest, Gijs van: Thermo-elastic optical coherence tomography. Optics Letters, no. 42, pp. 3466-3469, 2017
    BibTeX Link
    @article{HU_Wang2017,
       author = {Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Amenta, Gaetano and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and Soest, Gijs van},
       title = {Thermo-elastic optical coherence tomography},
       journal = {Optics Letters},
       volume = {42},
       number = {17},
       pages = {3466-3469},
       DOI = {10.1364/OL.42.003466},
       year = {2017},
       type = {Journal Article}
    }
    
  • Lange, Birgit and Jocham, Dieter and Brinkmann, Ralf and Cordes, Jens: Stone/tissue differentiation for Holmium laser lithotripsy using autofluorescence: Clinical proof of concept study. Lasers in Surgery and Medicine, no. 49, pp. 361-365, 2017
    BibTeX Link
    @article{Lange2017,
       author = {Lange, Birgit and Jocham, Dieter and Brinkmann, Ralf and Cordes, Jens},
       title = {Stone/tissue differentiation for Holmium laser lithotripsy using autofluorescence: Clinical proof of concept study},
       journal = {Lasers in Surgery and Medicine},
       volume = {49},
       number = {4},
       pages = {361-365},
       ISSN = {1096-9101},
       DOI = {10.1002/lsm.22611},
       year = {2017},
       type = {Journal Article}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Pfeiffer, Tom and Kolb, Jan Philip and Huber, Robert: Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup. in Proc. SPIE, no. 10414, pp. 1041403-1041403-7, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Pfeiffer, Tom and Kolb, Jan Philip and Huber, Robert},
       title = {Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup},
      booktitle = {Proc. SPIE},
       volume = {10414},
      year = {2017},
       pages = {1041403-1041403-7},
       url = {http://dx.doi.org/10.1117/12.2286035},
       type = {Conference Proceedings}
    }
    
  • Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber: Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate. Biomedical Optics Express, no. 8, pp. 3132-3142, OSA, Jul, 2017
    BibTeX Link
    @article{HU_2017_Eibla_BOE,
    author = {Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber},
    journal = {Biomedical Optics Express},
    number = {7},
    pages = {3132--3142},
    publisher = {OSA},
    title = {Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate},
    volume = {8},
    month = {Jul},
    year = {2017},
    doi = {10.1364/BOE.8.003132}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Shot-Noise Limited Time-Encoded Raman Spectroscopy. Journal of Spectroscopy, no. 2017, pp. 1-6, 2017
    BibTeX Link
    @article{Karpf2017,
       author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
       title = {Shot-Noise Limited Time-Encoded Raman Spectroscopy},
       journal = {Journal of Spectroscopy},
       volume = {2017},
       pages = {1-6},
       url = {https://doi.org/10.1155/2017/9253475},
       year = {2017},
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs: Short pulse laser induced thermo-elastic deformation imaging. in Proc. SPIE, no. 10062, pp. 100620C-100620C-4, 2017
    BibTeX Link
    @inproceedings{Wang2017,
       author = {Wang, Tianshi and Pfeiffer, Tom and Wu, Min and Wieser, Wolfgang and Draxinger, Wolfgang and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs},
       title = {Short pulse laser induced thermo-elastic deformation imaging},
    booktitle = Proc. SPIE},
      volume = {10062},
      year = {2017},
       pages = {100620C-100620C-4},
      url = {http://dx.doi.org/10.1117/12.2251502},
       type = {Conference Proceedings}
    }
    
    
  • Kepp, Timo and Koinzer, Stefan and Handels, Heinz and Brinkmann, Ralf: Registrierung von nicht sichtbaren Laserbehandlungsarealen der Retina in Live-Aufnahmen des Fundus. in Bildverarbeitung für die Medizin 2017: Algorithmen - Systeme - Anwendungen. Proceedings des Workshops vom 12. bis 14. März 2017 in Heidelberg, pp. 331-336, Springer Berlin Heidelberg, Berlin, Heidelberg, 2017
    BibTeX Link
    @inbook{Kepp2017,
       author = {Kepp, Timo and Koinzer, Stefan and Handels, Heinz and Brinkmann, Ralf},
       title = {Registrierung von nicht sichtbaren Laserbehandlungsarealen der Retina in Live-Aufnahmen des Fundus},
       booktitle = {Bildverarbeitung für die Medizin 2017: Algorithmen - Systeme - Anwendungen. Proceedings des Workshops vom 12. bis 14. März 2017 in Heidelberg},
       editor = {Maier-Hein, geb Fritzsche Klaus Hermann and Deserno, geb Lehmann Thomas Martin and Handels, Heinz and Tolxdorff, Thomas},
       publisher = {Springer Berlin Heidelberg},
       address = {Berlin, Heidelberg},
       pages = {331-336},
       ISBN = {978-3-662-54345-0},
       url = {http://dx.doi.org/10.1007/978-3-662-54345-0_74},
       year = {2017},
       type = {Book Section}
    }
    
  • Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon: Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]. Biomedical Optics Express, no. 8, pp. 1499-1511, 2017
    BibTeX Link
    @article{Pfäffle2017,
       author = {Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon},
       title = {Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {3},
       pages = {1499-1511},
       keywords = {Image reconstruction-restoration
    Optical coherence tomography},
       url = {http://www.osapublishing.org/boe/abstract.cfm?URI=boe-8-3-1499},
       year = {2017},
       type = {Journal Article}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert: Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging. in Proc. SPIE, no. 10083, pp. 100831C-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert},
       title = {Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging},
    booktitle = {Proc. SPIE },
       volume = {10083},
       pages = {100831C-6},
       year= {2017},
       doi= {10.1117/12.2251965},
       type = {Conference Proceedings}
    }
    
  • Matthias Eibl and Sebastian Karpf and Hubertus Hakert and Torben Blömker and Jan Philip Kolb and Christian Jirauschek and Robert Huber: Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification. Opt. Lett., no. 42, pp. 4406-4409, OSA, Nov, 2017
    BibTeX Link
    @article{2017_HU_Eibl_b_OL,
    author = {Matthias Eibl and Sebastian Karpf and Hubertus Hakert and Torben Bl\"{o}mker and Jan Philip Kolb and Christian Jirauschek and Robert Huber},
    journal = {Opt. Lett.},
    keywords = {NLI, TPEF, Multiphoton},
    number = {21},
    pages = {4406--4409},
    publisher = {OSA},
    title = {Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification},
    volume = {42},
    month = {Nov},
    year = {2017},
    doi = {10.1364/OL.42.004406},
    }
    
  • Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.: Power-controlled temperature guided retinal laser therapy. J Biomed Opt, no. 22, pp. 1-11, 2017
    BibTeX Link
    @article{Baade2017,
       author = {Baade, A. and von der Burchard, C. and Lawin, M. and Koinzer, S. and Schmarbeck, B. and Schlott, K. and Miura, Y. and Roider, J. and Birngruber, R. and Brinkmann, R.},
       title = {Power-controlled temperature guided retinal laser therapy},
       journal = {J Biomed Opt},
       volume = {22},
       number = {11},
       pages = {1-11},
       ISSN = {1083-3668},
       DOI = {10.1117/1.jbo.22.11.118001},
       year = {2017},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon: Off-axis reference beam for full-field swept-source OCT and holoscopy. Optics Express, no. 25, pp. 27770-27784, 2017
    BibTeX Link
    @article{Hillmann2017,
       author = {Hillmann, Dierck and Spahr, Hendrik and Sudkamp, Helge and Hain, Carola and Hinkel, Laura and Franke, Gesa and Hüttmann, Gereon},
       title = {Off-axis reference beam for full-field swept-source OCT and holoscopy},
       journal = {Optics Express},
       volume = {25},
       number = {22},
       pages = {27770-27784},
       DOI = {10.1364/OE.25.027770},
       year = {2017},
       type = {Journal Article}
    }
    
  • Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.: Noncontact holographic detection for photoacoustic tomography. J Biomed Opt, no. 22, pp. 1-14, 2017
    BibTeX Link
    @article{Buj2017,
       author = {Buj, C. and Munter, M. and Schmarbeck, B. and Horstmann, J. and Huttmann, G. and Brinkmann, R.},
       title = {Noncontact holographic detection for photoacoustic tomography},
       journal = {J Biomed Opt},
       volume = {22},
       number = {10},
       pages = {1-14},
       DOI = {10.1117/1.jbo.22.10.106007},
       year = {2017},
       type = {Journal Article}
    }
    
    
    
  • Pfeiffer, Tom and Draxinger, Wolfgang and Grill, C and Huber, R: Long-range live 3D-OCT at different spectral zoom levels. in Proc. SPIE, no. 10416, pp. 104160L1-4, 2017
    BibTeX
    @inproceedings{HU_Pfeiffer2017,
       author = {Pfeiffer, Tom and Draxinger, Wolfgang and Grill, C and Huber, R},
       title = {Long-range live 3D-OCT at different spectral zoom levels},
       booktitle = {Proc. SPIE},
       volume = {10416},
       pages = {104160L1-4},
       year = {2017},
       type = {Conference Proceedings}
    }
    
  • Maertz, J. and Mohler, K. J. and Kolb, J. P. and Kein, T. and Neubauer, A. and Kampik, A. and Priglinger, S. and Wieser, W. and Huber, R. and Wolf, A.: INTRAPAPILLARY PROLIFERATION IN OPTIC DISK PITS: Clinical Findings and Time-Related Changes. Retina, no. 37, pp. 906-914, 2017
    BibTeX Link
    @article{Maertz2017,
       author = {Maertz, J. and Mohler, K. J. and Kolb, J. P. and Kein, T. and Neubauer, A. and Kampik, A. and Priglinger, S. and Wieser, W. and Huber, R. and Wolf, A.},
       title = {INTRAPAPILLARY PROLIFERATION IN OPTIC DISK PITS: Clinical Findings and Time-Related Changes},
       journal = {Retina},
       volume = {37},
       number = {5},
       pages = {906-914},
       DOI = {10.1097/iae.0000000000001260},
       year = {2017},
       type = {Journal Article}
    }
    
  • Seiler, T. G. and Engler, M. and Beck, E. and Birngruber, R. and Kochevar, I. E.: Interface bonding with corneal crosslinking (CXL) after LASIK ex vivo. Investigative Ophthalmology and Visual Science, no. 58, pp. 6292-6298, 2017
    BibTeX Link
    @article{RN5042,
       author = {Seiler, T. G. and Engler, M. and Beck, E. and Birngruber, R. and Kochevar, I. E.},
       title = {Interface bonding with corneal crosslinking (CXL) after LASIK ex vivo},
       journal = {Investigative Ophthalmology and Visual Science},
       volume = {58},
       number = {14},
       pages = {6292-6298},
       DOI = {10.1167/iovs.17-22426},
       
       year = {2017},
       type = {Journal Article}
    }
    
  • Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation. International Journal of Nanomedicine, no. Volume 12, pp. 5659-5672, 2017
    BibTeX Link
    @article{Yao2017,
       author = {Yao, Cuiping and Rudnitzki, Florian and Hüttmann, Gereon and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
    journal = {International Journal of Nanomedicine},
       volume = {Volume 12},
       pages = {5659-5672},
       DOI = {10.2147/IJN.S140620},
       year = {2017},
       type = {Journal Article}
    }
    
  • Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.: Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea. Am J Pathol, no. 187, pp. 2451-2460, 2017
    BibTeX Link
    @article{Kretschmer2017,
       author = {Kretschmer, S. and Pieper, M. and Klinger, A. and Hüttmann, G. and Konig, P.},
       title = {Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea},
       journal = {Am J Pathol},
       ISSN = {0002-9440},
       DOI = {10.1016/j.ajpath.2017.07.006},
      volume = {187},
      number = {11},
       year = {2017},
    pages = {2451-2460},
       type = {Journal Article}
    }
    
  • Klein, Thomas and Huber, Robert: High-speed OCT light sources and systems [Invited]. Biomedical Optics Express, no. 8, pp. 828-859, 2017
    BibTeX Link
    @article{Klein2017,
       author = {Klein, Thomas and Huber, Robert},
       title = {High-speed OCT light sources and systems [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {2},
       pages = {828-859},
       abstract = {Imaging speed is one of the most important parameters that define the performance of optical coherence tomography (OCT) systems. During the last two decades, OCT speed has increased by over three orders of magnitude. New developments in wavelength-swept lasers have repeatedly been crucial for this development. In this review, we discuss the historical evolution and current state of the art of high-speed OCT systems, with focus on wavelength swept light sources and swept source OCT systems.},
       keywords = {Imaging systems
    Optical coherence tomography
    Lasers and laser optics
    Lasers, tunable},
       DOI = {10.1364/BOE.8.000828},
       year = {2017},
       type = {Journal Article}
    }
    
  • Laves, Max-Heinrich and Schoob, Andreas and Kahrs, Lüder A. and Pfeiffer, Tom and Huber, Robert and Ortmaier, Tobias: Feature tracking for automated volume of interest stabilization on 4D-OCT images. in Proc. SPIE, no. 10135, pp. 101350W-101350W-7, 2017
    BibTeX Link
    @inproceedings{Laves2017,
       author = {Laves, Max-Heinrich and Schoob, Andreas and Kahrs, Lüder A. and Pfeiffer, Tom and Huber, Robert and Ortmaier, Tobias},
       title = {Feature tracking for automated volume of interest stabilization on 4D-OCT images},
     booktitle = {Proc. SPIE},
       volume = {10135},
       pages = {101350W-101350W-7},
      year = {2017},
       url = {http://dx.doi.org/10.1117/12.2255090},
       type = {Conference Proceedings}
    }
    
  • Jirauschek, Christian and Huber, Robert: Efficient simulation of the swept-waveform polarization dynamics in fiber spools and Fourier domain mode-locked (FDML) lasers. Journal of the Optical Society of America B, no. 34, pp. 1135-1146, 2017
    BibTeX Link
      @article{Jirauschek2017,
       author = {Jirauschek, Christian and Huber, Robert},
       title = {Efficient simulation of the swept-waveform polarization dynamics in fiber spools and Fourier domain mode-locked (FDML) lasers},
       journal = {Journal of the Optical Society of America B},
       volume = {34},
       number = {6},
       pages = {1135-1146},
       abstract = {We present a theoretical model and its efficient numerical implementation for the simulation of wavelength-swept waveform propagation in fiber systems such as Fourier domain mode-locked (FDML) lasers, fully accounting for the polarization dynamics in fiber spools and further polarization-dependent optical components in the setup. This approach enables us to perform long-time simulations of the FDML laser dynamics over more than 100,000 cavity round trips, as required for some FDML configurations to ensure convergence to the steady-state operating regime. The model is validated against experimental results for single propagation through a fiber spool and for stationary FDML operation. The polarization dynamics due to the fiber spool, inducing polarization-mode dispersion, bending birefringence as well as cross-phase modulation, and other optical components such as the Faraday-rotating mirror used for polarization compensation is thoroughly investigated.},
       keywords = {Laser theory
    Lasers, tunable
    Optical coherence tomography
    Birefringence
    Polarization
    Pulses},
       url = {https://www.osapublishing.org/josab/abstract.cfm?uri=josab-34-6-1135},
       year = {2017},
       type = {Journal Article}
    }
    
  • Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.: Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects. J Vis Exp, no. 30, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Miura, Y. and Pruessner, J. and Mertineit, C. L. and Kern, K. and Muenter, M. and Moltmann, M. and Danicke, V. and Brinkmann, R.},
       title = {Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects},
       journal = {J Vis Exp},
       number = {124},
       ISSN = {1940-087x},
       DOI = {10.3791/54326},
       year = {2017},
       type = {Journal Article},
    volume = {30}
    
  • Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.: ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch`s Membrane and Causes Its Structural Remodeling. Curr Eye Res, no. 3, pp. 1-12, 2017
    BibTeX Link
    @article{Miura2017,
       author = {Rudolf, M. and Mir Mohi Sefat, A. and Miura, Y. and Tura, A. and Raasch, W. and Ranjbar, M. and Grisanti, S. and Aherrahrou, Z. and Wagner, A. and Messinger, J. D. and Garber, D. W. and Anantharamaiah, G. M. and Curcio, C. A.},
       title = {ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch's Membrane and Causes Its Structural Remodeling},
       journal = {Curr Eye Res},
       pages = {1-12},
       ISSN = {0271-3683},
       DOI = {10.1080/02713683.2017.1370118},
       year = {2017},
       type = {Journal Article},
    volume = {3}
    }
    
  • Pfeiffer, Tom and Draxinger, Wolfgang and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Huber, Robert: Analysis of FDML lasers with meter range coherence. in Proc. SPIE, no. 10053, pp. 100531T-100531T-6, 2017
    BibTeX
    @inproceedings{Pfeiffer2017,
       author = {Pfeiffer, Tom and Draxinger, Wolfgang and Wieser, Wolfgang and Klein, Thomas and Petermann, Markus and Huber, Robert},
       title = {Analysis of FDML lasers with meter range coherence},
       booktitle = {Proc. SPIE},
       volume = {10053},
       pages = {100531T-100531T-6},
      year = {2017},
       type = {Conference Proceedings}
    }
    
  • Verbytskyi, Ievgen and Münter, Michael and Buj, Christian and Brinkmann, Ralf: A Problem of a Displacement Calculation of Tissue Surface in Non-Contact Photoacoustic Tomography. Naukovi Visti NTUU KPI, pp. 58-64, 2017
    BibTeX Link
    @article{Verbytskyi2017,
       author = {Verbytskyi, Ievgen and Münter, Michael and Buj, Christian and Brinkmann, Ralf},
       title = {A Problem of a Displacement Calculation of Tissue Surface in Non-Contact Photoacoustic Tomography},
       journal = {Naukovi Visti NTUU KPI},
       number = {2},
       pages = {58-64},
       ISSN = {2519-8890},
       url = {http://dx.doi.org/10.20535/1810-0546.2017.2.98021},
       year = {2017},
       type = {Journal Article}
    }
    
  • Kolb, Jan Philip and Klee, Julian and Pfeiffer, Tom and Huber, Robert: 1060nm FDML laser with centimeter coherence length and 1.67 MHz sweep rate for full eye length and retinal ultra-widefield OCT. in Proc. SPIE, no. 10416, pp. 104160J-104160J-6, 2017
    BibTeX Link
    @inproceedings{Kolb2017,
       author = {Kolb, Jan Philip and Klee, Julian and Pfeiffer, Tom and Huber, Robert},
       title = {1060nm FDML laser with centimeter coherence length and 1.67 MHz sweep rate for full     eye length and retinal ultra-widefield OCT},
      booktitle = {Proc. SPIE},
       volume = {10416},
      pages = {104160J-104160J-6},
      year = {2017},
       url = {http://dx.doi.org/10.1117/12.2286854},
       type = {Conference Proceedings}
    }
    

2016

  • Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogel, Alfred: Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery. American Physical Society,Phys. Rev. B, no. 94, pp. 1-19, 2016
    BibTeX Link
    @article{Linz2016,
       author = {Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogel, Alfred},
       title = {Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery},
       journal = {American Physical Society,Phys. Rev. B},
       volume = { 94},
       number = {2},
       pages = {1-19},
       url = {http://link.aps.org/doi/10.1103/PhysRevB.94.024113},
       year = {2016},
       type = {Journal Article}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert: Two-photon microscopy using fiber-based nanosecond excitation. Biomedical Optics Express, no. 7, pp. 2432-2440, 2016
    BibTeX Link
    @article{Karpf2016,
       author = {Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert},
       title = {Two-photon microscopy using fiber-based nanosecond excitation},
       journal = {Biomedical Optics Express},
       volume = {7},
       number = {7},
       pages = {2432-2440},
       abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
       keywords = {Fiber optics imaging
    Nonlinear optics, fibers
    Lasers, fiber
    Fluorescence microscopy
    Nonlinear microscopy},
       DOI = {10.1364/BOE.7.002432},
       year = {2016},
       type = {Journal Article}
    }
    
  • Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.: Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. 1-48, 2016
    BibTeX Link
    @article{Schulz-Hildebrandt2016,
       author = {Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.},
       title = {Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {1-48},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584651},
       year = {2016},
       type = {Journal Article}
    }
    
  • Kang, S. and Park, Y. G. and Kim, J. R. and Seifert, E. and Theisen-Kunde, D and Brinkman, R and Roh, Y. J.: Selective Retina Therapy in Patients With Chronic Central Serous Chorioretinopathy: A Pilot Study. Medicine (Baltimore), no. 95, pp. e2524, 2016
    BibTeX Link
    @article{Kang1016,
       author = {Kang, S. and Park, Y. G. and Kim, J. R. and Seifert, E. and Theisen-Kunde, D and Brinkman, R and Roh, Y. J.},
       title = {Selective Retina Therapy in Patients With Chronic Central Serous Chorioretinopathy: A Pilot Study},
       journal = {Medicine (Baltimore)},
       volume = {95},
       number = {3},
       pages = {e2524},
       note = {1536-5964
    Kang, Seungbum
    Park, Young Gun
    Kim, Jae Ryun
    Seifert, Eric
    Dirk, Theisen-Kunde
    Ralf, Brinkmann
    Roh, Young Jung
    Journal Article
    United States
    Medicine (Baltimore). 2016 Jan;95(3):e2524. doi: 10.1097/MD.0000000000002524.},
       abstract = {We evaluated visual outcomes, changes of maximum macular thickness (MMT) and subretinal fluid (SRF), and safety in patients with chronic central serous chorioretinopathy (CSC) after treatment with selective retina therapy (SRT). Retrospective cohort study of patients with chronic CSC presenting to a university-based hospital from January 2014 through January 2015 was conducted. A total of 12 eyes of 12 patients with chronic CSC lasting for at least 3 months was recruited. The follow-up period ranged from 3 to 12 months. Following evaluation of test spots at temporal arcades, SRT (Q-switched neodymium-doped yttrium lithium fluoride [Nd:YLF] laser; wavelength, 527 nm, pulse duration, 1.7 microsececond) was applied to the surrounding areas of leakage observed on fluorescein angiogram and/or pigment epithelial detachment (PED). Changes in best-correct visual acuity (BCVA), MMT, and SRF and macular sensitivity (MS) by microperimetry (MP) were evaluated. Eyes received treatment in a mean of 3.83 spots at the pulse energy of 65 to 90 muJ. Mean BCVA (logMAR) improved from 0.23 +/- 0.12 at baseline to 0.14 +/- 0.13 at 3 months. MMT decreased from 341.4 +/- 85.5 mum at baseline to 236.0 +/- 57.9 mum at 3 months. SRF completely resolved in 75% (9 eyes) at 3 months. Large PEDs (2 eyes) were flattened at 3 months. Retreatment was performed in 4 eyes. MP showed no evidence of scotoma around SRT-treated lesions. SRT treatment targeting the surrounding area of leakage point showed favorable visual and structural outcomes in chronic CSC patients without the risk of scotoma.},
       ISSN = {0025-7974},
       DOI = {10.1097/md.0000000000002524},
       year = {2016},
       type = {Journal Article}
    }
    
  • Park, Y. G. and Kim, J. R. and Kang, S. and Seifert, E. and Theisen-Kunde, D. and Brinkmann, R. and Roh, Y. J.: Safety and efficacy of selective retina therapy (SRT) for the treatment of diabetic macular edema in Korean patients. Graefes Arch Clin Exp Ophthalmol, 2016
    BibTeX Link
    @article{Park2016,
       author = {Park, Y. G. and Kim, J. R. and Kang, S. and Seifert, E. and Theisen-Kunde, D. and Brinkmann, R. and Roh, Y. J.},
       title = {Safety and efficacy of selective retina therapy (SRT) for the treatment of diabetic macular edema in Korean patients},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       note = {1435-702x
    Park, Young Gun
    Kim, Jae Ryun
    Kang, Seungbum
    Seifert, Eric
    Theisen-Kunde, Dirk
    Brinkmann, Ralf
    Roh, Young-Jung
    Journal article
    Graefes Arch Clin Exp Ophthalmol. 2016 Jan 23.},
       abstract = {PURPOSE: Selective retina therapy (SRT) stimulates retinal pigment epithelium (RPE) cell migration and proliferation into irradiated areas. The objective of this study was to evaluate the efficacy and safety of SRT in Korean patients with clinically significant diabetic macular edema (DME). METHODS: Prospective non-randomized interventional case series study. Twenty-three eyes of 21 patients with clinically significant DME were treated with SRT and followed for 6 months. Patients underwent an evaluation of best corrected visual acuity (BCVA) in Early Treatment Diabetic Retinopathy Study (ETDRS) letters. Microperimetry was employed to measure macular sensitivity within the central 10 degrees field, and the central macular thickness (CMT) and maximum macular thickness (MMT) were measured. RESULTS: An improvement in BCVA of one to two ETDRS lines was observed in 41.2 % of patients and an improvement of greater than two lines in 29.4 %. Although there was no significant change in CMT (P > 0.05), MMT decreased from 465.8 +/- 87.4 mum to 434.3 +/- 83.9 mum (P = 0.006), and mean macular sensitivity increased from 20.8 +/- 3.4dB to 22.5 +/- 3.5dB (P = 0.02). CONCLUSIONS: The gains in BCVA and improvement in macular sensitivity demonstrated that SRT may be used as an effective and safe treatment modality in Korean patients with clinically significant DME.},
       keywords = {Diabetic macular edema
    Dosimetry
    Microperimetry
    Retinal pigment epithelium
    Selective retina therapy},
       ISSN = {0721-832x},
       DOI = {10.1007/s00417-015-3262-1},
       year = {2016},
       type = {Journal Article}
    }
    
  • Yasui, Ayako and Yamamoto, Manabu and Hirayama, Kumiko and Shiraki, Kunihiko and Theisen-Kunde, Dirk and Brinkmann, Ralf and Miura, Yoko and Kohno, Takeya: Retinal sensitivity after selective retina therapy (SRT) on patients with central serous chorioretinopathy. Graefe`s Archive for Clinical and Experimental Ophthalmology, pp. 1-12, 2016
    BibTeX Link
    @article{Yasui2016,
       author = {Yasui, Ayako and Yamamoto, Manabu and Hirayama, Kumiko and Shiraki, Kunihiko and Theisen-Kunde, Dirk and Brinkmann, Ralf and Miura, Yoko and Kohno, Takeya},
       title = {Retinal sensitivity after selective retina therapy (SRT) on patients with central serous chorioretinopathy},
       journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
       pages = {1-12},
       abstract = {To assess retinal sensitivity after selective retina therapy (SRT) in patients with central serous chorioretinopathy (CSCR).},
       ISSN = {1435-702X},
       url = {http://dx.doi.org/10.1007/s00417-016-3441-8},
       year = {2016},
       type = {Journal Article}
    }
    
  • Kim, H. D. and Jang, S. Y. and Lee, S. H. and Kim, Y. S. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.: Retinal Pigment Epithelium Responses to Selective Retina Therapy in Mouse Eyes. Invest Ophthalmol Vis Sci, no. 57, pp. 3486-95, 2016
    BibTeX Link
    @article{Kim2016,
       author = {Kim, H. D. and Jang, S. Y. and Lee, S. H. and Kim, Y. S. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.},
       title = {Retinal Pigment Epithelium Responses to Selective Retina Therapy in Mouse Eyes},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {57},
       number = {7},
       pages = {3486-95},
       abstract = {PURPOSE: To investigate the characteristics of retinal pigment epithelium (RPE) and retinal damage induced by selective retina therapy (SRT) in mice, and to elucidate longitudinal changes in RPE cells. METHODS: C57BL/6J mice received SRT and continuous-wave laser photocoagulation (cwPC). The cell death pattern was evaluated using TUNEL assay, and proliferative potential of the RPE cells was evaluated using 5-ethynyl-2'-dexoyuridine (EdU) assay. To investigate the cell-cell integrity of RPE cells, beta-catenin staining was performed. The number and hexagonality of RPE cells in the SRT-treated area were estimated using a Voronoi diagram with time periods of 3 hours to 14 days. Antibodies to microphthalmia-associated transcription factor (MiTF) and orthodenticle homeobox 2 (Otx2) were used to confirm the specific characteristics of RPE cells in the SRT-treated area. RESULTS: The number of TUNEL-positive cells located in the neural retina was significantly lower in lesions treated with SRT compared to those treated with cwPC. EdU-positive RPE cells were first detected 3 to 12 hours after SRT, and increased until 3 to 7 days after SRT. beta-catenin staining showed that hexagonality was compromised and subsequently, RPE cells expanded in size within the targeted location. The number of RPE cells in SRT lesions decreased gradually until 12 hours after SRT and recovered by 14 days. Upregulated expression of MiTF and Otx2 was observed for 2 weeks in the SRT lesions. CONCLUSIONS: Selective retina therapy seems to induce selective RPE damage without collateral thermal injury in the neural retina. Furthermore, SRT-treated lesions recovered by proliferation of RPE cells that were present in the treated lesions and by expansion of adjacent RPE cells.},
       ISSN = {0146-0404},
       DOI = {10.1167/iovs.16-19508},
       year = {2016},
       type = {Journal Article}
    }
    
  • Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf: Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results. Investigative Ophthalmology & Visual Science, no. 57, pp. 5852-5852, 2016
    BibTeX Link
    @article{Hüttmann2016,
       author = {Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf},
       title = {Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {57},
       number = {12},
       pages = {5852-5852},
       abstract = {Abstract Purpose : The molecular processes during heating with a photocoagulation laser, particularly in sub-visible or mere thermal stimulation treatment, have only partly been understood, and different theories exist that try to explain its clinical efficacy. Optical coherence tomography (OCT) was successfully used to grade lesions with high accuracy 1 hour after the treatments and beyond. During the irradiation, changes in tissue scattering and, by use of the Doppler signal, tissue motion caused by thermal expansion and coagulation-induced tissue contraction were shown to correlate ex-vivo and in rabbits with the strength of photocoagulation lesions. Aim of this study was to validate feasibility and reproducibility of these results in humans. Methods : In an ongoing study more than 100 lesions of three patients have been imaged with a slitlamp-based OCT (1060 nm, 90,000 A-scans/s) with varying irradiance during laser exposure. Durations of the exposure were 50 ms and 200 ms; spot size was 300 µm. Eye movements and heart beat were corrected by cross-correlation of the images. Increased tissue scattering and movement of the neuronal retina due to thermal expansion were determined from the image sequences with 3 ms temporal resolution. Results : In the first treatments with this prototype device, we received acceptable image quality in 1/3 of the lesions. Changes in the neuronal retina were successful visualized during and after the laser irradiation, demonstrating the feasibility of a real-time assessment of initial effects of photocoagulation in humans. Lesion visibility in standard, reflection-based OCT was much weaker during treatment compared to 1 hour afterwards. Increased tissue scattering was observed in stronger lesions already during the laser irradiation. At reduced irradiance, scattering increase was only observed after the end of irradiation. However, tissue motion towards the vitreous was still observed in these cases. Conclusions : In conclusion, high-speed OCT recording during photocoagulation measures initial tissue changes during photocoagulation in humans. It may enhance our understanding of the tissue dynamics right after laser irradiation. It may provide useful information for a real-time dosage control as well. This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2016},
       type = {Journal Article}
    }
    
  • Ranjbar, Mahdy and Brinkmann, Max Philipp and Tura, Aysegül and Rudolf, Martin and Miura, Yoko and Grisanti, Salvatore: Ranibizumab interacts with the VEGF-A/VEGFR-2 signaling pathway in human RPE cells at different levels. Cytokine, no. 83, pp. 210-216, 2016
    BibTeX Link
    @article{Ranjbar2016,
       author = {Ranjbar, Mahdy and Brinkmann, Max Philipp and Tura, Aysegül and Rudolf, Martin and Miura, Yoko and Grisanti, Salvatore},
       title = {Ranibizumab interacts with the VEGF-A/VEGFR-2 signaling pathway in human RPE cells at different levels},
       journal = {Cytokine},
       volume = {83},
       pages = {210-216},
       abstract = {Vascular endothelial growth factor (VEGF) secreted by the retinal pigment epithelium (RPE) plays an important role in ocular homeostasis, but also in diseases, most notably age-related macular degeneration (AMD). To date, anti-VEGF drugs like ranibizumab have been shown to be most effective in treating these pathologic conditions. However, clinical trials suggest that the RPE could degenerate and perish through anti-VEGF treatment. Herein, we evaluated possible pathways and outcomes of the interaction between ranibizumab and human RPE cells (ARPE-19). Results indicate that ranibizumab affects the VEGF-A metabolism in RPE cells from an extra- as well as intracellular site. The drug is taken up into the cells, with the VEGF receptor 2 (VEGFR-2) being involved, and decreases VEGF-A protein levels within the cells as well as extracellularly. Oxidative stress plays a key role in various inflammatory disorders of the eye. Our results suggest that oxidative stress inhibits RPE cell proliferation. This anti-proliferative effect on RPE cells is significantly enhanced through ranibizumab, which does not inhibit RPE cell proliferation substantially in absence of relevant oxidative stress. Therefore, we emphasize that anti-VEGF treatment should be selected carefully in AMD patients with preexistent extensive RPE atrophy.},
       keywords = {Ranibizumab
    RPE
    VEGF-A
    VEGFR-2
    Oxidative stress},
       ISSN = {1043-4666},
       url = {http://www.sciencedirect.com/science/article/pii/S1043466616300722},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 2: Praktische Aspekte und Anwendung. Augenheilkunde up2date, no. 6, pp. 305-320, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 2: Praktische Aspekte und Anwendung},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {305-320},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-117459},
       year = {2016},
       type = {Journal Article}
    }
    
  • Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.: OCT verstehen – Teil 1: Physikalische Grundlagen. Augenheilkunde up2date, no. 6, pp. 289-300, 2016
    BibTeX Link
    @article{Horstmann2016,
       author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
       title = {OCT verstehen – Teil 1: Physikalische Grundlagen},
       journal = {Augenheilkunde up2date},
       volume = {6},
       number = {04},
       pages = {289-300},
       ISSN = {1616-9719},
       DOI = {10.1055/s-0042-113337},
       year = {2016},
       type = {Journal Article}
    }
    
  • Schlott, Kerstin and Koinzer, Stefan and Baade, Alexander and Birngruber, Reginald and Roider, Johann and Brinkmann, Ralf: Lesion strength control by automatic temperature guided retinal photocoagulation. Journal of Biomedical Optics, no. 21, pp. 098001-098001, 2016
    BibTeX Link
    @article{Schlott2016,
       author = {Schlott, Kerstin and Koinzer, Stefan and Baade, Alexander and Birngruber, Reginald and Roider, Johann and Brinkmann, Ralf},
       title = {Lesion strength control by automatic temperature guided retinal photocoagulation},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {9},
       pages = {098001-098001},
       note = {10.1117/1.JBO.21.9.098001},
       abstract = {Abstract.  Laser photocoagulation is an established treatment for a variety of retinal diseases. However, when using the same irradiation parameter, the size and strength of the lesions are unpredictable due to unknown inter- and intraindividual optical properties of the fundus layers. The aim of this work is to investigate a feedback system to generate desired lesions of preselectable strengths by automatically controlling the irradiation time. Optoacoustics were used for retinal temperature monitoring. A 532-nm continuous wave Nd:YAG laser was used for photocoagulation. A 75-ns/523-nm Q-switched Nd:YLF laser simultaneously excited temperature-dependent pressure transients, which were detected at the cornea by an ultrasonic transducer embedded in a contact lens. The temperature data were analyzed during the irradiation by a LabVIEW routine. The treatment laser was switched off automatically when the required lesion strength was achieved. Five different feedback control algorithms for different lesion sizes were developed and tested on rabbits in vivo. With a laser spot diameter of 133  μm, five different lesion types with ophthalmoscopically visible diameters ranging mostly between 100 and 200  μm, and different appearances were achieved by automatic exposure time control. The automatically controlled lesions were widely independent of the treatment laser power and the retinal pigmentation.},
       ISSN = {1083-3668},
       DOI = {10.1117/1.JBO.21.9.098001},
       year = {2016},
       type = {Journal Article}
    }
    
  • Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.: Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis. Pneumologie, no. 70, pp. SOP2, 2016
    BibTeX Link
    @article{Pieper2016,
       author = {Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.},
       title = {Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis},
       journal = {Pneumologie},
       volume = {70},
       number = {07},
       pages = {SOP2},
       ISSN = {0934-8387},
       DOI = {10.1055/s-0036-1584654},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin: Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy. Journal of Biomedical Optics, no. 21, pp. 078001-078001, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
       title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
       journal = {Journal of Biomedical Optics},
       volume = {21},
       number = {7},
       pages = {078001-078001},
       note = {10.1117/1.JBO.21.7.078001},
       abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
       ISSN = {1083-3668},
       year = {2016},
       type = {Journal Article}
    }
    
  • Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon: In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography. Optics Letters, no. 41, pp. 4987-4990, 2016
    BibTeX Link
    @article{Sudkamp2016,
       author = {Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon},
       title = {In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography},
       journal = {Optics Letters},
       volume = {41},
       number = {21},
       pages = {4987-4990},
       abstract = {With a simple setup, mainly composed of a low coherence light source and a camera, full-field optical coherence tomography (FF-OCT) allows volumetric tissue imaging. However, fringe washout constrains its use in retinal imaging. Here, we present a novel motion-insensitive approach to FF-OCT, which introduces path-length differences between the reference and the sample light in neighboring pixels using an off-axis reference beam. The temporal carrier frequency in scanned time-domain OCT is replaced by a spatial carrier frequency. Volumetric in-vivo FF-OCT measurements of the human retina were acquired in only 1.3 s, comparable to the acquisition times of current clinically used OCT devices.},
       keywords = {Imaging systems
    Coherence imaging
    Optical coherence tomography
    Frequency filtering
    Interferometric imaging},
    DOI = {10.1364/OL.41.004987}
     url= {https://www.osapublishing.org/ol/abstract.cfm?uri=ol-41-21-4987}  
       year = {2016},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon: In vivo optical imaging of physiological responses to photostimulation in human photoreceptors. PNAS Early Edition, pp. 1-6, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
       title = {In vivo optical imaging of physiological responses to photostimulation in human photoreceptors},
       journal = {PNAS Early Edition},
       pages = {1-6},
       abstract = {Noninvasive functional imaging of molecular and cellular processes of vision may have immense impact on research and clinical diagnostics. Although suitable intrinsic optical signals (IOSs) have been observed ex vivo and in immobilized animals in vivo, detecting IOSs of photoreceptor activity in living humans was cumbersome and time consuming. Here, we observed clear spatially and temporally resolved changes in the optical path length of the photoreceptor outer segment as a response to an optical stimulus in the living human eye. To witness these changes, we evaluated phase data obtained with a parallelized and computationally aberration-corrected optical coherence tomography system. The noninvasive detection of optical path length changes shows neuronal photoreceptor activity of single cones in living human retina, and therefore, it may provide diagnostic options in ophthalmology and neurology and could provide insights into visual phototransduction in humans.},
       url = {http://www.pnas.org/content/early/2016/10/10/1606428113.abstract},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam-Peters, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs: Heartbeat OCT and Motion-Free 3D In Vivo Coronary Artery Microscopy. JACC: Cardiovascular Imaging, no. 9, pp. 622-623, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, Tianshi and Pfeiffer, Tom and Regar, Evelyn and Wieser, Wolfgang and van Beusekom, Heleen and Lancee, Charles T. and Springeling, Geert and Krabbendam-Peters, Ilona and van der Steen, Antonius F. W. and Huber, Robert and van Soest, Gijs},
       title = {Heartbeat OCT and Motion-Free 3D In Vivo Coronary Artery Microscopy},
       journal = {JACC: Cardiovascular Imaging},
       volume = {9},
       number = {5},
       pages = {622-623},
       note = {10.1016/j.jcmg.2015.08.010},
       abstract = {Intravascular optical coherence tomography (IV-OCT) has gained widespread use over the past few years, offering highly detailed images of coronary artery pathologies and interventions (1). In contrast to the cross-sectional view, longitudinal sections and 3-dimensional (3D) renderings are affected by cardiac motion artifacts and undersampling, complicating interpretation and measurements (2). We developed Heartbeat OCT, a new OCT method that achieves up to 4,000 frames/s imaging speed for isotropically sampled volume datasets acquired within the diastolic phase of 1 cardiac cycle to restore 3D IV-OCT image fidelity. In this research letter, we present the first in vivo data acquired with this new experimental technology.},
       keywords = {OPTICAL COHERENCE TOMOGRAPHY; 2ND },
       ISSN = {1936-878X},
       DOI = {10.1016/j.jcmg.2015.08.010},
       year = {2016},
       type = {Journal Article}
    }
    
  • Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.: Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept. Cell Physiol Biochem, no. 38, pp. 737-47, 2016
    BibTeX Link
    @article{Ranjbar2016,
       author = {Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.},
       title = {Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept},
       journal = {Cell Physiol Biochem},
       volume = {38},
       number = {2},
       pages = {737-47},
       note = {1421-9778
    Ranjbar, Mahdy
    Brinkmann, Max Philipp
    Zapf, Dorinja
    Miura, Yoko
    Rudolf, Martin
    Grisanti, Salvatore
    Journal Article
    Switzerland
    Cell Physiol Biochem. 2016;38(2):737-47. doi: 10.1159/000443030. Epub 2016 Feb 15.},
       abstract = {BACKGROUND/AIMS: VEGF-A is induced by oxidative stress, and functions as a survival factor for various cell types, including retinal pigment epithelial (RPE) cells. Anti-vascular endothelial growth factor (VEGF) drugs like aflibercept and bevacizumab have shown to be most effective in treating neovascular age-related macular degeneration (AMD), however uptake of the drugs might lead to interference with cell physiology. Herein, we evaluated the significance of the Fc receptor (FcR) within this context and moreover explored the impact of VEGF inhibition under normal conditions as well as under oxidative stress, in terms of potential adverse effects. METHODS: ARPE-19 (human RPE) cells were treated with aflibercept and bevacizumab in presence or absence of H2O2 as oxidative stress stimulus. After 24h cells were evaluated for drug uptake, VEGF-A expression and secretion, levels of intracellular reactive oxygen species (ROS) as well as cell proliferation. Experiments were repeated with cells being pre-incubated with an FcR inhibitor prior to drug application. RESULTS: Both drugs inhibited extracellular levels of VEGF-A and were taken up into the RPE, resulting in significantly reduced intracellular levels of VEGF-A. When oxidative stress was applied, intracellular ROS levels in cells treated with both drugs rose, and cell proliferation was reduced. Prior incubation with the FcR inhibitor lessened the uptake of bevacizumab, but not aflibercept into RPE cells, and simultaneously enhanced cell survival under oxidative stress conditions. CONCLUSIONS: Our results indicate that uptake and accumulation of aflibercept and bevacizumab within RPE cells affect the intracellular VEGF-A metabolism negatively, leading to a biologically relevant reduced cell survival under oxidative stress. The FcR plays a substantial role in the uptake of bevacizumab, but not aflibercept, which allows an enhanced RPE cell survival through FcR blockage in an environment dominated by oxidative stress, as clinically significant for various inflammatory retinal disorders.},
       ISSN = {1015-8987},
       DOI = {10.1159/000443030},
       year = {2016},
       type = {Journal Article}
    }
    
  • Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.: Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b. J Cancer Res Clin Oncol, no. 142, pp. 1261-71, 2016
    BibTeX Link
    @article{Maushagen2016,
       author = {Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.},
       title = {Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b},
       journal = {J Cancer Res Clin Oncol},
       volume = {142},
       number = {6},
       pages = {1261-71},
       note = {1432-1335
    Maushagen, Regina
    Reers, Stefan
    Pfannerstill, Ann-Christin
    Hahlbrock, Angelina
    Stauber, Roland
    Rahmanzadeh, Ramtin
    Rades, Dirk
    Pries, Ralph
    Wollenberg, Barbara
    Journal Article
    Germany
    J Cancer Res Clin Oncol. 2016 Jun;142(6):1261-71. doi: 10.1007/s00432-016-2150-3. Epub 2016 Apr 1.},
       abstract = {PURPOSE: Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to beta-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet. METHODS: The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies. RESULTS: Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome. CONCLUSION: Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.},
       keywords = {Apoptosis
    Caspase 9b
    Caspases
    Head and neck cancer
    Paclitaxel},
       ISSN = {0171-5216},
       DOI = {10.1007/s00432-016-2150-3},
       year = {2016},
       type = {Journal Article}
    }
    
    
  • Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.: Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT. Klin Monatsbl Augenheilkd, no. 233, pp. 1324-1330, 2016
    BibTeX Link
    @article{Spahr2016,
       author = {Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.},
       title = {Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT},
       journal = {Klin Monatsbl Augenheilkd},
       volume = {233},
       number = {12},
       pages = {1324-1330},
       ISSN = {0023-2165},
       DOI = {10.1055/s-0042-120279},
       year = {2016},
       type = {Journal Article}
    }
    
  • Tromberg, B. J. and Anderson, R. R. and Birngruber, R. and Brinkmann, R. and Berns, M. W. and Parrish, J. A. and Apiou-Sbirlea, G.: Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health. J Biomed Opt, no. 21, pp. 124001, 2016
    BibTeX Link
    @article{RN5034,
       author = {Tromberg, B. J. and Anderson, R. R. and Birngruber, R. and Brinkmann, R. and Berns, M. W. and Parrish, J. A. and Apiou-Sbirlea, G.},
       title = {Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health},
       journal = {J Biomed Opt},
       volume = {21},
       number = {12},
       pages = {124001},
       ISSN = {1560-2281 (Electronic)
    1083-3668 (Linking)},
       DOI = {10.1117/1.JBO.21.12.124001},   
       year = {2016},
       type = {Journal Article}
    }
    
  • Huber, Robert and Dworak, Lars and Moser, Jacques E. and Grätzel, Michael and Wachtveitl, Josef: Beyond Vibrationally Mediated Electron Transfer: Coherent Phenomena Induced by Ultrafast Charge Separation. The Journal of Physical Chemistry C, no. 120, pp. 8534-8539, 2016
    BibTeX Link
    @article{Huber2016,
       author = {Huber, Robert and Dworak, Lars and Moser, Jacques E. and Grätzel, Michael and Wachtveitl, Josef},
       title = {Beyond Vibrationally Mediated Electron Transfer: Coherent Phenomena Induced by Ultrafast Charge Separation},
       journal = {The Journal of Physical Chemistry C},
       volume = {120},
       number = {16},
       pages = {8534-8539},
       ISSN = {1932-7447},
       DOI = {10.1021/acs.jpcc.6b02012},
       year = {2016},
       type = {Journal Article}
    }
    
  • Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon: Aberration-free volumetric high-speed imaging of in vivo retina. Scientific Reports, no. 6, pp. 1-11, 2016
    BibTeX Link
    @article{Hillmann2016,
       author = {Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon},
       title = {Aberration-free volumetric high-speed imaging of in vivo retina},
       journal = {Scientific Reports},
       volume = {6},
       pages = {1-11},
       url = {http://dx.doi.org/10.1038/srep35209},
       year = {2016},
       type = {Journal Article}
    }
    
  • Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.: A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67. Sci Rep, no. 6, pp. 27032, 2016
    BibTeX Link
    @article{Wang2016,
       author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
       title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
       journal = {Sci Rep},
       volume = {6},
       pages = {27032},
       note = {2045-2322
    Wang, Sijia
    Huttmann, Gereon
    Scholzen, Thomas
    Zhang, Zhenxi
    Vogel, Alfred
    Hasan, Tayyaba
    Rahmanzadeh, Ramtin
    Journal Article
    England
    Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
       abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
       ISSN = {2045-2322},
       DOI = {10.1038/srep27032},
       year = {2016},
       type = {Journal Article}
    }