Publikationen 2010 - 2009

2010

  • Miura, Y and Klettner, A and Roider, J: VEGF antagonists decrease barrier function of retinal pigment epithelium in vitro: possible participation of intracellular glutathione. Invest Ophthalmol Vis Sci, no. 51, pp. 4848-55, 2010
    BibTeX Link
    @article{Miura2010,
       author = {Miura, Y and Klettner, A and Roider, J},
       title = {VEGF antagonists decrease barrier function of retinal pigment epithelium in vitro: possible participation of intracellular glutathione},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {51},
       number = {9},
       pages = {4848-55},
       note = {Miura, Yoko
    Klettner, Alexa
    Roider, Johann
    United States
    Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4848-55. Epub 2010 Apr 30.},
       abstract = {PURPOSE: To investigate the influence of VEGF antagonists on the barrier function of the retinal pigment epithelium and underlying mechanisms. METHODS: Porcine RPE cells were cultured on six-well membrane inserts. The cells were exposed to bevacizumab (62.5 microg/mL) or ranibizumab (25 microg/mL) for 24 hours (short term) or 9 days (long term). Transepithelial flux of FITC-dextran and intracellular levels of reduced glutathione (GSH) at normal and low-glucose conditions were investigated at different points in time. The influence of the addition of triamcinolone acetonide (TA) was investigated. The effect of GSH depletion on RPE permeability was examined using L-buthionine sulfoximine (BSO), a gamma-glutamylcysteine synthethase inhibitor. RESULTS: After short-term exposure, VEGF antagonists increased the transepithelial flux of FITC-dextran significantly on day 2. Bevacizumab, but not ranibizumab, increased permeability up to 9 days. Under long-term exposure, both drugs enhanced permeability for 7 days; bevacizumab had the stronger effect. The addition of TA inhibited this increase. At the ninth day of short- and long-term exposure, bevacizumab-exposed cells, but not ranibizumab-exposed cells, exhibited a significantly lower GSH level. In the low-glucose condition, both drugs accelerated the decrease of intracellular GSH for the first 48 hours. GSH depletion increased the permeability of retinal pigment epithelium. TA had no effect on BSO-induced GSH depletion. CONCLUSIONS: The results suggest that bevacizumab and ranibizumab may decrease RPE barrier function, with bevacizumab exhibiting a prolonged and more profound effect. Combination with TA is thought to be beneficial because of its protective effect on stabilizing RPE junctional integrity.},
       keywords = {Angiogenesis Inhibitors/ pharmacology
    Animals
    Antibodies, Monoclonal/ pharmacology
    Antibodies, Monoclonal, Humanized
    Buthionine Sulfoximine/pharmacology
    Cell Membrane Permeability/drug effects
    Cells, Cultured
    Dextrans/pharmacokinetics
    Enzyme Inhibitors/pharmacology
    Fluorescein-5-isothiocyanate/analogs & derivatives/pharmacokinetics
    Glucose/pharmacology
    Glutathione/ metabolism
    Retinal Pigment Epithelium/cytology/ drug effects/ metabolism
    Swine
    Tight Junctions/drug effects/metabolism
    Vascular Endothelial Growth Factor A/ antagonists & inhibitors/metabolis
    AutoPhoN},
       ISSN = {1552-5783 (Electronic)
    0146-0404 (Linking)},
       DOI = {10.1167/iovs.09-4699},
       year = {2010},
       type = {Journal Article}
    }
    
  • Mueller, M. and Schulz-Wackerbarth, C. and Steven, P. and Lankenau, E. and Bonin, T. and Mueller, H. and Brueggemann, A. and Birngruber, R. and Grisanti, S. and Huettmann, G.: Slit-lamp-adapted fourier-domain OCT for anterior and posterior segments: preliminary results and comparison to time-domain OCT. Curr Eye Res, no. 35, pp. 722-32, 2010
    BibTeX
    @article{Müller,
       author = {Mueller, M. and Schulz-Wackerbarth, C. and Steven, P. and Lankenau, E. and Bonin, T. and Mueller, H. and Brueggemann, A. and Birngruber, R. and Grisanti, S. and Huettmann, G.},
       title = {Slit-lamp-adapted fourier-domain OCT for anterior and posterior segments: preliminary results and comparison to time-domain OCT},
       journal = {Curr Eye Res},
       volume = {35},
       number = {8},
       pages = {722-32},
       note = {Using Smart Source Parsing
    Aug},
       abstract = {PURPOSE: To evaluate the diagnostic potential of a slit-lamp (SL)-adapted Fourier-domain (= spectral radar, SR) optical coherence tomography (OCT)-SL-SR-OCT-instrument as an in vivo imaging device for use in examinations of the anterior and posterior segments. MATERIALS AND METHODS: In a pilot study, 88 eyes from 70 healthy volunteers and patients were examined using a prototype Fourier-domain SL-SR-OCT system. Results were compared to those from the following commercially available systems: the 1310-nm SL-OCT (Heidelberg Engineering, Heidelberg, Germany) for anterior segment and the Stratus OCT (Zeiss Meditec, Jena, Germany) for posterior segment imaging. Our SL-SR-OCT provides 1025 axial scans, 5000 Hz line-scan frequency, scan length of up to 8 mm, axial depth in air of 3.5 mm, and resolution of 9 mum. For posterior visualization, a hand-held 78-diopter ophthalmoscopic lens was used. RESULTS: Our SL-SR-OCT system allowed simultaneous scanning with direct biomicroscopic and SL imaging of anterior and posterior segment structures. Anatomical structures and pathological changes were displayed with high resolution and excellent contrast. Measurements of corneal and retinal thickness were possible. In comparison to images obtained by the SL-OCT, our SL-SR-OCT boasted a higher resolution, thus providing more clinically relevant details of the corneal epithelium, internal structure of filtering blebs, etc. Complete imaging of the chamber angle was limited, however, due to the backscattering properties of the sclera at 830 nm. For posterior segment imaging, excellent delineation of the macula and optic nerve head details, with a distinct portrayal of macular pathology and retinal edema, was possible with SL-SR-OCT. CONCLUSION: SL-SR-OCT enables detailed imaging of physiological and pathological anterior and posterior segment structures. As a multi-purpose device, it offers a wide spectrum of applications, with high-quality OCT-imaging, in a comfortable setting without the need to move the patient.},
       year = {2010}
    }
  • Prahs, P. and Walter, A. and Regler, R. and Theisen-Kunde, D. and Birngruber, R. and Brinkmann, R. and Framme, C.: Selective retina therapy (SRT) in patients with geographic atrophy due to age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol, no. 248, pp. 651-8, 2010
    BibTeX Link
    @article{Prahs2010,
       author = {Prahs, P. and Walter, A. and Regler, R. and Theisen-Kunde, D. and Birngruber, R. and Brinkmann, R. and Framme, C.},
       title = {Selective retina therapy (SRT) in patients with geographic atrophy due to age-related macular degeneration},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {248},
       number = {5},
       pages = {651-8},
       note = {1435-702x
    Prahs, Philipp
    Walter, Andreas
    Regler, Roman
    Theisen-Kunde, Dirk
    Birngruber, Reginald
    Brinkmann, Ralf
    Framme, Carsten
    Journal Article
    Germany
    Graefes Arch Clin Exp Ophthalmol. 2010 May;248(5):651-8. doi: 10.1007/s00417-009-1208-1. Epub 2009 Dec 22.},
       abstract = {BACKGROUND: For geographic atrophy (GA) due to age-related macular degeneration (AMD) there is so far no approved treatment option. Usually, increased autofluorescence (AF) levels of different patterns adjacent to the atrophic area indicate lipofuscin-laden retinal pigment epithelium (RPE) cells at a high risk for apoptosis. Herein, SRT was used to selectively treat these cells to stimulate RPE proliferation, in order to reduce or ideally stop further growth of the atrophic area. MATERIAL AND METHODS: Six eyes of six patients with bilateral equally pronounced GA were treated by SRT, while the fellow eye served as control. Irradiation was performed using a prototype SRT laser (Medical Laser Center Lubeck, Nd:YLF laser; 527 nm; 200 ns/1.7 micros pulse duration; 30 repetitive pulses at 100 Hz). Test lesions with increasing energies were applied at the lower vessel arcade to determine the individual angiographic and ophthalmoscopic threshold radiant exposures. Treatment was then performed in the area of increased AF adjacent to the GA using energies between both thresholds. The GA progression rates of treated and fellow eyes were evaluated. RESULTS: After a 1-year follow-up, a progression of the atrophic area was observed in the treated eyes (0.7-8.0 mm(2)/yr, mean 3.0 mm(2)/yr; 46%/yr) whereas the progression rates of the fellow eyes were insignificantly lower (0.46-4.04 mm(2)/yr, mean 1.9 mm(2)/yr; 30%/yr; p = 0.134). The progression rate in the treated eyes of two patients increased significantly, while in the other four patients, the progression rates were nearly the same between both eyes. Moreover, one of these two eyes showed an unexpected RPE reaction after treatment, since all laser lesions led to RPE atrophy and thus an accelerated enlargement of the GA occurred. CONCLUSION: SRT in the hyperautofluorescent areas of GA was not able to stop or slow down the progression of GA. However, modified treatment strategies might be more promising, e.g. placing the spots outside the hyperautofluorescent areas where RPE apoptosis is postulated. Moreover, SRT studies on GA might be more successfully performed on specific subgroups of GA, based on autofluorescence and other findings.},
       keywords = {Aged
    Aged, 80 and over
    Disease Progression
    Fluorescein Angiography
    Fluorescence
    Follow-Up Studies
    Geographic Atrophy/etiology/physiopathology/*surgery
    Humans
    Image Processing, Computer-Assisted
    *Laser Coagulation
    Lasers, Solid-State/therapeutic use
    Lipofuscin/metabolism
    Macular Degeneration/complications/physiopathology/*surgery
    Ophthalmologic Surgical Procedures/*methods
    Pilot Projects
    Prognosis
    Retinal Pigment Epithelium/metabolism},
       ISSN = {0721-832x},
       DOI = {10.1007/s00417-009-1208-1},
       year = {2010},
       type = {Journal Article}
    }
    
  • Roider, J. and Liew, S. H. and Klatt, C. and Elsner, H. and Poerksen, E. and Hillenkamp, J. and Brinkmann, R. and Birngruber, R.: Selective retina therapy (SRT) for clinically significant diabetic macular edema. Graefes Arch Clin Exp Ophthalmol, no. 248, pp. 1263-72, 2010
    BibTeX
    @article{Roider,
       author = {Roider, J. and Liew, S. H. and Klatt, C. and Elsner, H. and Poerksen, E. and Hillenkamp, J. and Brinkmann, R. and Birngruber, R.},
       title = {Selective retina therapy (SRT) for clinically significant diabetic macular edema},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {248},
       number = {9},
       pages = {1263-72},
       note = {Using Smart Source Parsing
    Sep},
       abstract = {PURPOSE: To test selective retina therapy (SRT) as a treatment of clinically significant diabetic macular edema (DME). METHODS: Prospective two-center interventional uncontrolled phase II pilot study. Thirty-nine eyes of 39 patients with previously untreated non-ischemic DME were treated with focal laser treatment using a Q-switched frequency doubled Nd:YLF laser which selectively affects the retinal pigment epithelium while sparing the photoreceptor layer. Optoacoustic measurements, fundus fluorescein angiography (FFA), and funduscopy were used to determine the individual threshold of RPE damage of each patient. The pulse energy was adjusted to apply angiographically visible but funduscopically invisible effects. Optoacoustic measurements were correlated with funduscopy and FFA. Follow-up examinations at 3 and 6 months post-treatment included best-corrected ETDRS visual acuity (BCVA), FFA, fundus photography, and retinal thickness measured by optical coherence tomography. The primary outcome measure was change of BCVA. Other outcome measures were change of retinal thickness, presence of hard exudates, leakage in FFA, accuracy of optoacoustic measurements, and correlation of BCVA with change of anatomical and systemic parameters. RESULTS: Mean BCVA improved from 43.7 letters (standard deviation, SD=9.1) at baseline to 46.1 letters (SD=10.5) at the 6-month follow-up (p=0.02). BCVA improved (>5 letters) or remained stable (+/-5 letters) in 84% of eyes. Thirteen percent of eyes improved by > or =10 letters, while 16% of eyes lost more than 5 letters. There was no severe loss of vision (> or =15 letters). Overall, retinal thickness, hard exudates, and leakage in FFA did not change significantly (p> 0.05), while improvement of BCVA correlated with a reduction of hard exudates (p=0.01) and central retinal thickness (p=0.01). Specificity and sensitivity of detecting the angiographic visible threshold of RPE damage by optoacoustic measurements were 86% and 70% respectively. No adverse effects or pain were noted during or after treatment. Conclusions Functional and anatomical improvement or stabilization was observed in most patients. SRT appears to be safe. Optoacoustic measurements accurately detect the individual threshold of RPE damage. A randomized trial is required to further test efficacy and safety of SRT as a treatment of clinically significant diabetic macular edema (DME).},
       year = {2010}
    }
    
  • Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S: Photodynamic therapy to treat ovarian cancer. no. 5, National Cancer Institute (NCI) Alliance for Nanotechnology, 2010
    BibTeX
    @misc{Hasan,
       author = {Hasan, T and Rai, T and Spring, B and Zheng, X. and Abu-Yousif, A and Rahmanzadeh, R and Verma, S},
       title = {Photodynamic therapy to treat ovarian cancer},
       publisher = {National Cancer Institute (NCI) Alliance for Nanotechnology},
       volume = {5},
       number = {4},
       year = {2010}
    }
  • Bombien, R. and Lesche, C. and Lozonschi, L. and Feucker, M. and Brinkmann, R. and Dahmen, C. and Schunke, M. and Cremer, J. and Lutter, G.: Percutaneous Aortic Valve Replacement: Emerging Tractability for Sufficient Intracardiac Resection of the Aortic Valve. Innovations (Phila), no. 5, pp. 55-59, 2010
    BibTeX
    @article{Bombien,
       author = {Bombien, R. and Lesche, C. and Lozonschi, L. and Feucker, M. and Brinkmann, R. and Dahmen, C. and Schunke, M. and Cremer, J. and Lutter, G.},
       title = {Percutaneous Aortic Valve Replacement: Emerging Tractability for Sufficient Intracardiac Resection of the Aortic Valve},
       journal = {Innovations (Phila)},
       volume = {5},
       number = {1},
       pages = {55-59},
       note = {Philadelphia, Pa.
    Innovations (Phila). 2010 Jan;5(1):55-59.},
       abstract = {OBJECTIVE:: The feasibility of endovascular resection of highly calcified aortic valves has already been demonstrated by our group. Different endovascular and intracardiac tractability methods were applied. In this study, these technologies were analyzed comparing the tractability, the resection time, and the lesions in the surrounding tissue. METHODS:: All aortic valve resections (seven human hearts and 21 porcine hearts) were performed using a Thulium:YAG laser (continuous wave, wavelength of 2.01 mum, 20 watts power rating). In the first resection system, the laser fiber was controlled by a free in-lying flexible endoscope (O 2.5 mm, length of 600 mm). The distal part of the endoscope (40 mm) was moved in one plane by proximal manual control (three degrees of freedom). The resection system was separated into defined rooms assigning one room for one tool. The fiber was controlled by the above-mentioned endoscope (*) (three degrees of freedom). The third resection system was a mechanical microactuator carrying the laser fiber (three degrees of freedom). The fourth resection system contains a rotatable inlay with defined rooms and a newly designed nitinol (NiTi) microactuator that controlled the laser fiber (four degrees of freedom). The resection time per leaflet was measured in minutes. Gross anatomy and histology in the surrounding tissue were evaluated. RESULTS:: The resection time in approaches 1, 2, 3, and 4 was 5.5 +/- 2.3 minutes, 7.4 +/- 2.7 minutes, +/- 6.6 minutes, and2.3 +/- 1.2 minutes, respectively. The gross anatomy and histology of collateral damages revealed only superficial lesions of the surrounding tissue. The amount of lesions and the resection time were lower in the fourth approach with four degrees of freedom. CONCLUSIONS:: This analysis demonstrated that a precise tractability with four degrees of freedom is necessary for a faster and safer endovascular resection of the aortic valve. The analysis will help to optimize the ongoing development of the endovascular and intracardiac resection technology.},
       year = {2010}
    }
  • Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon: Optical coherence tomography with online visualization of more than seven rendered volumes per second. Journal of Biomedical Optics, no. 15, pp. 026014, 2010
    BibTeX
    @article{Probst2010,
       author = {Probst, Joachim and Hillmann, Dierck and Lankenau, Eva and Winter, Christan and Oelckers, Stefan and Koch, Peter and Huttmann, Gereon},
       title = {Optical coherence tomography with online visualization of more than seven rendered volumes per second},
       journal = {Journal of Biomedical Optics},
       volume = {15},
       number = {2},
       pages = {026014},
       keywords = {image resolution
    medical image processing
    optical tomography
    rendering (computer graphics)
    surgery},
       year = {2010}
    }
  • Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.: Optical coherence tomography for process control of laser micromachining. Rev Sci Instrum, no. 81, pp. 033705, 2010
    BibTeX
    @article{Wiesner2010,
       author = {Wiesner, M. and Ihlemann, J. and Muller, H. H. and Lankenau, E. and Huttmann, G.},
       title = {Optical coherence tomography for process control of laser micromachining},
       journal = {Rev Sci Instrum},
       volume = {81},
       number = {3},
       pages = {033705},
       note = {Using Smart Source Parsing
    Mar},
       abstract = {In situ surface imaging for nondestructive evaluation (NDE) by optical coherence tomography (OCT) before, during, and after ablative laser processing is presented. Furthermore, it is shown that the ability of in situ characterization is beneficial for samples such as optical fibers, which are difficult to handle in the standard analysis. Surface images taken by the OCT are compared with these common analysis tools such as scanning electron microscopy (SEM), reflected-light, and confocal microscopy. An axial resolution of approximately 126 nm for surface detection and a lateral resolution <2.5 microm are obtained and the potential of the setup to imaging structures with high aspect ratio is demonstrated.},
       year = {2010}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second. Optics express, no. 18, pp. 14685-704, jul,, 2010
    BibTeX Link
    @Article{HU_2010_Wieser_b,
      Title                    = {{Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2010},
    
      Month                    = jul,
      Number                   = {14},
      Pages                    = {14685--704},
      Volume                   = {18},
    
      Doi                      = {10.1364/OE.18.014685},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen,Oettingenstr. 67, 80538 Munich, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-14-14685}
    }
  • Todor, Sebastian and Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert: Linewidth Optimization of Fourier Domain Mode-Locked Lasers. Conference on Lasers and Electro-Optics 2010, pp. CMW7 1-3, OSA, Washington, D.C., 2010
    BibTeX Link
    @Article{HU_2010_Todor_a,
      Title                    = {{Linewidth Optimization of Fourier Domain Mode-Locked Lasers}},
      Author                   = {Todor, Sebastian and Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert},
      Journal                  = {Conference on Lasers and Electro-Optics 2010},
      Year                     = {2010},
      Pages                    = {CMW7 1--3 },
    
      Address                  = {Washington, D.C.},
      Doi                      = {10.1364/CLEO.2010.CMW7},
      ISBN                     = {978-1-55752-889-6},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO-2010-CMW7}
    }
  • Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.: Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer. Cancer Res, no. 70, pp. 9234-42, 2010
    BibTeX
    @article{Rahmanzadeh2010,
       author = {Rahmanzadeh, R. and Rai, P. and Celli, J. P. and Rizvi, I. and Baron-Luhr, B. and Gerdes, J. and Hasan, T.},
       title = {Ki-67 as a molecular target for therapy in an in vitro three-dimensional model for ovarian cancer},
       journal = {Cancer Res},
       volume = {70},
       number = {22},
       pages = {9234-42},
       note = {Using Smart Source Parsing
    Nov 15; Epub 2010 Nov 2},
       abstract = {Targeting molecular markers and pathways implicated in cancer cell growth is a promising avenue for developing effective therapies. Although the Ki-67 protein (pKi-67) is a key marker associated with aggressively proliferating cancer cells and poor prognosis, its full potential as a therapeutic target has never before been successfully shown. In this regard, its nuclear localization presents a major hurdle because of the need for intracellular and intranuclear delivery of targeting and therapeutic moieties. Using a liposomally encapsulated construct, we show for the first time the specific delivery of a Ki-67-directed antibody and subsequent light-triggered death in the human ovarian cancer cell line OVCAR-5. Photoimmunoconjugate-encapsulating liposomes (PICEL) were constructed from anti-pKi-67 antibodies conjugated to fluorescein 5(6)-isothiocyanate, as a photoactivatable agent, followed by encapsulation in noncationic liposomes. Nucleolar localization of the PICELs was confirmed by confocal imaging. Photodynamic activation with PICELs specifically killed pKi-67-positive cancer cells both in monolayer and in three-dimensional (3D) cultures of OVCAR-5 cells, with the antibody TuBB-9 targeting a physiologically active form of pKi-67 but not with MIB-1, directed to a different epitope. This is the first demonstration of (a) the exploitation of Ki-67 as a molecular target for therapy and (b) specific delivery of an antibody to the nucleolus in monolayer cancer cells and in an in vitro 3D model system. In view of the ubiquity of pKi-67 in proliferating cells in cancer and the specificity of targeting in 3D multicellular acini, these findings are promising and the approach merits further investigation.},
       year = {2010}
    }
  • Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s. Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432., 2010
    BibTeX
    @book{Bonin2010,
       author = {Bonin, T. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s},
       publisher = {Opt Lett. 2010 Oct 15;35(20):3432-4. doi: 10.1364/OL.35.003432.},
       abstract = {In vivo full-field (FF) optical coherence tomography (OCT) images of human retina are presented by using a rapidly tunable laser source in combination with an ultra-high-speed camera. Fourier-domain FF-OCT provided a way to increase the speed of retinal imaging by parallel acquisition of A-scans. Reduced contrast caused by cross talk was observed only below the retinal pigment epithelium. With a 100Hz sweep rate, FF-OCT was fast enough to acquire OCT images with acceptable motion artifacts. FF-OCT allows ultrafast retinal imaging, boosting image speed by a lack of moving parts and a considerably higher irradiation power.},
      year={2010}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: High-Quality 3-D Imaging with Multimegahertz OCT. Optics and Photonics News, no. 21, pp. 28 - 29, OSA, dec,, 2010
    BibTeX Link
    @Article{HU_2010_Wieser_a,
      Title                    = {{High-Quality 3-D Imaging with Multimegahertz OCT}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics and Photonics News},
      Year                     = {2010},
    
      Month                    = dec,
      Number                   = {12},
      Pages                    = {28 -- 29 },
      Volume                   = {21},
    
      Doi                      = {10.1364/OPN.21.12.000028},
      ISSN                     = {1047-6938},
      Publisher                = {OSA},
      Url                      = {http://www.osa-opn.org/abstract.cfm?URI=opn-21-12-28 http://www.opticsinfobase.org/abstract.cfm?URI=OPN-21-12-28}
    }
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: High-power FDML laser for swept source-OCT at 1060 nm. in SPIE Photonics Europe, pp. 77150B-77150B-5, International Society for Optics and Photonics, apr,, 2010
    BibTeX Link
    @InProceedings{HU_2010_Marschall_b,
      Title                    = {{High-power FDML laser for swept source-OCT at 1060 nm}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Booktitle                = {SPIE Photonics Europe},
      Year                     = {2010},
      Editor                   = {Popp, J\"{u}rgen and Drexler, Wolfgang and Tuchin, Valery V and Matthews, Dennis L},
      Month                    = apr,
      Pages                    = {77150B--77150B--5},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.854238},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1345306}
    }
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Hsu, Kevin and Hansen, Kim P and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: Fourier domain mode-locked swept source at 1050 nm based on a tapered amplifier. Optics express, no. 18, pp. 15820-31, jul,, 2010
    BibTeX Link
    @Article{HU_2010_Marschall_a,
      Title                    = {{Fourier domain mode-locked swept source at 1050 nm based on a tapered amplifier}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Hsu, Kevin and Hansen, Kim P and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Journal                  = {Optics express},
      Year                     = {2010},
    
      Month                    = jul,
      Number                   = {15},
      Pages                    = {15820--31},
      Volume                   = {18},
    
      Doi                      = {10.1364/OE.18.015820},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-18-15-15820}
    }
    
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, Götz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E: FDML swept source at 1060 nm using a tapered amplifier. in BiOS, pp. 75541H-75541H-5, International Society for Optics and Photonics, feb,, 2010
    BibTeX Link
    @InProceedings{HU_2010_Marschall_c,
      Title                    = {{FDML swept source at 1060 nm using a tapered amplifier}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Hsu, Kevin and Sumpf, Bernd and Hasler, Karl-Heinz and Erbert, G\"{o}tz and Jensen, Ole B and Pedersen, Christian and Huber, Robert and Andersen, Peter E},
      Booktitle                = {BiOS},
      Year                     = {2010},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {75541H--75541H--5},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.842011},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1343844}
    }
  • Framme, C. and Panagakis, G. and Birngruber, R.: Effects on Choroidal Neovascularization after Anti-VEGF Upload Using Intravitreal Ranibizumab, as Determined by Spectral Domain-Optical Coherence Tomography. Investigative Ophthalmology & Visual Science, no. 51, pp. 1671-1676, 2010
    BibTeX Link
    @article{Framme2010,
       author = {Framme, C. and Panagakis, G. and Birngruber, R.},
       title = {Effects on Choroidal Neovascularization after Anti-VEGF Upload Using Intravitreal Ranibizumab, as Determined by Spectral Domain-Optical Coherence Tomography},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {51},
       number = {3},
       pages = {1671-1676},
       note = {563VW
    Times Cited:18
    Cited References Count:17},
       abstract = {PURPOSE. It is unclear whether anti-VEGF monotherapy in age-related macular degeneration (AMD) achieves morphologic CNV regression or only stops further CNV growth. In this study, spectral domain-optical coherence tomography (SD-OCT) was used to image CNV structure before and after anti-VEGF treatment.
    METHODS. Out of 107 consecutive patients, a prospective CNV evaluation was possible in 78 of them. Newly diagnosed CNV (classic CNV: n = 16; occult CNV: n = 54; minimal classic CNV: n = 8) due to AMD was imaged before and 4 weeks after anti-VEGF upload in three intravitreal injections of ranibizumab. Qualitative (structural changes) and quantitative measurements (diameter and thickness) of the CNV were obtained from the OCT images.
    RESULTS. Classic CNV components were observed above the RPE/photoreceptor complex, whereas occult CNVs stayed below. Of all postoperative OCTs, 59% revealed complete dry retinal structures, 27% showed reduced edema, and 14% showed edema remaining unchanged. Mean macular thickness decreased significantly from 427 to 303 mu m (P = 0.000). Qualitatively, overall CNV architecture appeared to be unchanged in 78%, was reduced in thickness in 18%, and became larger in 4%. Quantitatively, in all CNV subtypes, the diameter of the CNV lesions (preoperative, 2813 mu m; postoperative, 2804 mu m) did not change after treatment (classic CNV: P = 0.390; occult CNV: P = 0.405, minimal classic CNV: P = 0.092) independent of postoperative retinal edema. The overall thickness of the lesion, however, was reduced from 205 to 175 mu m (P = 0.000). Thickness reduction was significantly enhanced especially in CNV with classic components (n = 24; 252 to 197 mu m; P = 0.000; reduction, 22%), whereas reduction was smaller but also significant in occult CNV (183 to 164 mu m; P = 0.003; reduction, 10%).
    CONCLUSIONS. With SD-OCT, CNV size can be two-dimensionally determined and followed up after intravitreal anti-VEGF treatment. In only 4% of CNV was enlargement observed, whereas in 78%, CNV architecture appeared qualitatively unchanged, independent of retinal edema. Quantitative measurements underlined stable CNV diameters for all subtypes but revealed significant reduction of thickness especially for classic CNV components. In this series, ranibizumab monotherapy was able to morphologically stop further CNV growth but, in most patients, did not lead to a major regression of CNV, especially of its occult components. (Invest Ophthalmol Vis Sci. 2010;51:1671-1676) DOI:10.1167/iovs.09-4496},
       keywords = {macular degeneration
    therapy
    verteporfin
    oct},
       ISSN = {0146-0404},
       DOI = {Doi 10.1167/Iovs.09-4496},
       url = {<Go to ISI>://WOS:000275164300060},
       year = {2010},
       type = {Journal Article}
    }
    
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Direct measurement of the instantaneous linewidth of rapidly wavelength-swept lasers. Optics letters, no. 35, pp. 3733-5, nov,, 2010
    BibTeX Link
    @Article{HU_2010_Biedermann_a,
      Title                    = {{Direct measurement of the instantaneous linewidth of rapidly wavelength-swept lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Journal                  = {Optics letters},
      Year                     = {2010},
    
      Month                    = nov,
      Number                   = {22},
      Pages                    = {3733--5},
      Volume                   = {35},
    
      Doi                      = {10.1364/OL.35.003733},
      ISSN                     = {1539-4794},
      Url                      = {http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-35-22-3733}
    }
    
  • Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.: Development and applications of photo-triggered theranostic agents. Adv Drug Deliv Rev, no. 62, pp. 1094-124, 2010
    BibTeX
    @article{Rai,
       author = {Rai, P. and Mallidi, S. and Zheng, X. and Rahmanzadeh, R. and Mir, Y. and Elrington, S. and Khurshid, A. and Hasan, T.},
       title = {Development and applications of photo-triggered theranostic agents},
       journal = {Adv Drug Deliv Rev},
       volume = {62},
       number = {11},
       pages = {1094-124},
       note = {Rai, Prakash
    Mallidi, Srivalleesha
    Zheng, Xiang
    Rahmanzadeh, Ramtin
    Mir, Youssef
    Elrington, Stefan
    Khurshid, Ahmat
    Hasan, Tayyaba
    Nihms238162
    Adv Drug Deliv Rev. 2010 Aug 30;62(11):1094-124. Epub 2010 Sep 19.},
       abstract = {Theranostics, the fusion of therapy and diagnostics for optimizing efficacy and safety of therapeutic regimes, is a growing field that is paving the way towards the goal of personalized medicine for the benefit of patients. The use of light as a remote-activation mechanism for drug delivery has received increased attention due to its advantages in highly specific spatial and temporal control of compound release. Photo-triggered theranostic constructs could facilitate an entirely new category of clinical solutions which permit early recognition of the disease by enhancing contrast in various imaging modalities followed by the tailored guidance of therapy. Finally, such theranostic agents could aid imaging modalities in monitoring response to therapy. This article reviews recent developments in the use of light-triggered theranostic agents for simultaneous imaging and photoactivation of therapeutic agents. Specifically, we discuss recent developments in the use of theranostic agents for photodynamic-, photothermal- or photo-triggered chemotherapy for several diseases.},
       keywords = {Animals
    Anti-Infective Agents/diagnostic use/therapeutic use
    Antineoplastic Agents/diagnostic use/therapeutic use
    Diagnostic Imaging/ methods
    Drug Carriers/diagnostic use/therapeutic use
    Humans
    Infection/ diagnosis/ drug therapy
    Nanoparticles/diagnostic use/therapeutic use
    Neoplasms/ diagnosis/drug therapy/ therapy
    Phototherapy/ methods},
       year = {2010}
    }
    
    
  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {Using Smart Source Parsing
    Epub 2009 Oct 29},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       year = {2010}
    }
  • Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.: Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Ophthalmic Res, no. 43, pp. 122-33, 2010
    BibTeX Link
    @article{Miura2009,
       author = {Miura, Y. and Klettner, A. and Noelle, B. and Hasselbach, H. and Roider, J.},
       title = {Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture},
       journal = {Ophthalmic Res},
       volume = {43},
       number = {3},
       pages = {122-33},
       note = {1423-0259
    Miura, Yoko
    Klettner, Alexa
    Noelle, Bernhard
    Hasselbach, Heike
    Roider, Johann
    Journal Article
    Switzerland
    Ophthalmic Res. 2010;43(3):122-33. doi: 10.1159/000252979. Epub 2009 Oct 29.},
       abstract = {AIMS: To evaluate the changes of morphological and functional characteristics of the retinal pigment epithelium (RPE)-choroid perfusion culture during cultivation. METHODS: PorcineRPE-choroid tissue was cultivated in a perfusion tissue culture system. After the indicated times, histology, immunolocalization of collagen IV and von Willebrand factor, RPE cell viability with calcein-AM, TUNEL assay and occludin immunolocalization of RPE cells were examined. The tissue was treated with selective RPE treatment laser after different time periods and the wound healing response was characterized. Vascular endothelial growth factor secretion was measured by enzyme-linked immunosorbent assay. RESULTS: On day 8, prominent morphological degenerative changes of RPE cells were observed in histology. According to the immunohistochemistry for collagen IV, the Bruch's membrane did not display any obvious decomposition until day 8. Von Willebrand factor staining decreased during cultivation, especially at the choriocapillaris. Calcein-AM staining and TUNEL assay displayed the increase of apoptotic changes in only a minority of the cells on day 4, but in many cells on day 8. Occludin delocalization was observed on day 8. Selective RPE treatment laser-produced wounds were completely closed by monolayer RPE when wounded on fresh and 3-day-old cultures, but not when wounded on 6-day-old cultures. Vascular endothelial growth factor secretion was stable between days 2 and 5, but increased after that. CONCLUSION: Under the stated culture perfusion conditions, porcine RPE-choroid tissue was suitable for experimentation up to 5 days of maintenance.},
       keywords = {Animals
    *Apoptosis
    Bruch Membrane/pathology
    Cell Survival
    Choroid/metabolism/*pathology/surgery
    Collagen Type IV/metabolism
    Enzyme-Linked Immunosorbent Assay
    Fluoresceins/metabolism
    Immunoenzyme Techniques
    In Situ Nick-End Labeling
    Laser Therapy
    Membrane Proteins/metabolism
    Occludin
    Organ Culture Techniques
    Retinal Pigment Epithelium/metabolism/*pathology/surgery
    Swine
    Time Factors
    Vascular Endothelial Growth Factor A/metabolism
    Wound Healing
    von Willebrand Factor/metabolism},
       ISSN = {0030-3747},
       DOI = {10.1159/000252979},
       year = {2010},
       type = {Journal Article}
    }
    

2009

  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Wavelength swept ASE source. in European Conferences on Biomedical Optics, pp. 73720O-73720O-6, International Society for Optics and Photonics, jul,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Eigenwillig_c,
      Title                    = {{Wavelength swept ASE source}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Andersen, Peter E and Bouma, Brett E},
      Month                    = jul,
      Pages                    = {73720O--73720O--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.831831},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=781146}
    }
  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Wavelength swept amplified spontaneous emission source. Optics express, no. 17, pp. 18794-807, oct,, 2009
    BibTeX Link
    @Article{HU_2009_Eigenwillig_a,
      Title                    = {{Wavelength swept amplified spontaneous emission source}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {18794--807},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.018794},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen Oettingenstr 67, 80538 Munich, Germany.},
      ISSN                     = {1094-4087}
    }
  • Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert: Ultra-rapid dispersion measurement in optical fibers. Optics express, no. 17, pp. 22871-8, dec,, 2009
    BibTeX Link
    @Article{HU_2009_Wieser_a,
      Title                    = {{Ultra-rapid dispersion measurement in optical fibers}},
      Author                   = {Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = dec,
      Number                   = {25},
      Pages                    = {22871--8},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.022871},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-25-22871}
    }
  • Gora, Michalina and Karnowski, Karol and Szkulmowski, Maciej and Kaluzny, Bartlomiej J and Huber, Robert and Kowalczyk, Andrzej and Wojtkowski, Maciej: Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range. Optics Express, no. 17, pp. 14880-14894, aug,, 2009
    BibTeX Link
    @Article{HU_2009_Gora_a,
      Title                    = {{Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range}},
      Author                   = {Gora, Michalina and Karnowski, Karol and Szkulmowski, Maciej and Kaluzny, Bartlomiej J and Huber, Robert and Kowalczyk, Andrzej and Wojtkowski, Maciej},
      Journal                  = {Optics Express},
      Year                     = {2009},
    
      Month                    = aug,
      Number                   = {17},
      Pages                    = {14880--14894 },
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.014880},
      Institution              = {Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5/7, PL-87-100 Toruń, Poland.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-17-14880}
    }
  • Hüttmann, G.: Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review. Klinische Monatsblätter für Augenheilkunde, no. 226, pp. 958, 2009
    BibTeX
    @article{Hüttmann2009,
       author = {Hüttmann, G.},
       title = {Übersicht der apparativen Entwicklungen in der optischen Kohärenztomografie: von der Darstellung der Retina zur Unterstützung therapeutischer Eingriffe. Optical Coherence Tomography: from Retina Imaging to Intraoperative Use- a Review},
       journal = {Klinische Monatsblätter für Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958},
       year = {2009}
    }
  • Miura, Y. and Roider, J.: Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells. Graefes Arch Clin Exp Ophthalmol, no. 247, pp. 641-9, 2009
    BibTeX
    @article{Miura2009,
       author = {Miura, Y. and Roider, J.},
       title = {Triamcinolone acetonide prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {247},
       number = {5},
       pages = {641-9},
       note = {Using Smart Source Parsing
    May; Epub 2009 Feb 3},
       abstract = {PURPOSE: Oxidative stress is known to disrupt the integrity of retinal pigment epithelium (RPE) tight junctions. The goal of this study is to evaluate the effect of triamcinolone acetonide (TA) on the junctional integrity of RPE under oxidative stress and to identify the underlying mechanisms. METHODS: Second passage porcine RPE cells were cultured on 6-well membrane inserts until 4 weeks after reaching confluence. Cells were incubated with TA (10(-5) M) for 30 min. FITC-containing medium was added to the upper chamber (cell's apical side). The cells were then challenged with 1 mM Hydrogen Peroxide (H(2)O(2)). After 5 h, the fluorescence intensity of the medium from lower chamber (cell's basolateral side) was measured using a fluorescence spectrofluorophotometer. This transepithelial flux of FITC-dextran was measured until the 21st day. The immunolocalization of occludin and F-actin was examined with fluorescence microscope. Reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio was determined by a colorimetric assay kit. RESULTS: Non-lethal oxidative stress by H(2)O(2) increased transepithelial flux of FITC-dextran significantly. TA inhibited this increase and preserved the lower flux through the whole experimental period. This permeability change by H(2)O(2) was reversible and recovered to the normal level within 3 weeks. In immunohistological study, H(2)O(2) reduced linear occludin staining at the cell border and increased actin stress fibers. TA prevented H(2)O(2)-induced disruption of junctional assembly of occludin and F-actin. Glutathione assay demonstrated that intracellular GSH/GSSG ratio decreased significantly with H(2)O(2), while TA preserved this ratio by up-regulating GSH synthesis. CONCLUSIONS: TA has a protective effect against oxidative stress-induced disruption of RPE tight junction by preserving cellular redox state.},
       year = {2009}
    }
  • Karnowski, Karol and Gora, Michalina and Kaluzny, Bartlomiej and Huber, Robert and Szkulmowski, Maciej and Kowalczyk, Andrzej and Wojtkowski, Maciej: Swept source OCT imaging of human anterior segment at 200 kHz. in SPIE BiOS: Biomedical Optics, pp. 716308-716308-7, International Society for Optics and Photonics, feb,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Karnowski_a,
      Title                    = {{Swept source OCT imaging of human anterior segment at 200 kHz}},
      Author                   = {Karnowski, Karol and Gora, Michalina and Kaluzny, Bartlomiej and Huber, Robert and Szkulmowski, Maciej and Kowalczyk, Andrzej and Wojtkowski, Maciej},
      Booktitle                = {SPIE BiOS: Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Manns, Fabrice and S\"{o}derberg, Per G and Ho, Arthur},
      Month                    = feb,
      Pages                    = {716308--716308--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.808555},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1331711}
    }
  • Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert: Subharmonic Fourier domain mode locking. Optics Letters, no. 34, pp. 725 - 727, mar,, 2009
    BibTeX Link
    @Article{HU_2009_Eigenwillig_b,
      Title                    = {{Subharmonic Fourier domain mode locking}},
      Author                   = {Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2009},
    
      Month                    = mar,
      Number                   = {6},
      Pages                    = {725 -- 727 },
      Volume                   = {34},
    
      Doi                      = {10.1364/OL.34.000725},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-34-6-725}
    }
  • Framme, C. and Walter, A. and Prahs, P. and Regler, R. and Theisen-Kunde, D. and Alt, C. and Brinkmann, R.: Structural changes of the retina after conventional laser photocoagulation and selective retina treatment (SRT) in spectral domain OCT. Curr Eye Res, no. 34, pp. 568-79, 2009
    BibTeX
    @article{Framme,
       author = {Framme, C. and Walter, A. and Prahs, P. and Regler, R. and Theisen-Kunde, D. and Alt, C. and Brinkmann, R.},
       title = {Structural changes of the retina after conventional laser photocoagulation and selective retina treatment (SRT) in spectral domain OCT},
       journal = {Curr Eye Res},
       volume = {34},
       number = {7},
       pages = {568-79},
       note = {Framme, Carsten
    Walter, Andreas
    Prahs, Philipp
    Regler, Roman
    Theisen-Kunde, Dirk
    Alt, Clemens
    Brinkmann, Ralf
    Research Support, Non-U.S. Gov't
    England
    Current eye research
    Curr Eye Res. 2009 Jul;34(7):568-79.},
       abstract = {BACKGROUND: Spectral domain optical coherence tomography (SD-OCT) in patients can deliver retinal cross-sectional images with high resolution. This may allow the evaluation of the extent of damage to the retinal pigment epithelium (RPE) and the neurosensory retina after laser treatment. This article aims to investigate the value of SD-OCT in comparing laser lesions produced by conventional laser photocoagulation and selective retina treatment (SRT). MATERIAL AND METHODS: In a retrospective study, conventional retinal laser (CRL) lesions and SRT laser lesions were evaluated with SD-OCT. One hundred seventy-five CRL lesions were investigated in 10 patients with diabetic maculopathy at timepoints between 1 hr and 4 years after treatment. Ninety-one SRT lesions were examined in 9 patients with central serous retinopathy, geographic atrophy, and diabetic maculopathy at timepoints between 1 hr and 2 years. CRL lesions were applied with an ophthalmoscopically slightly grayish-white appearance (Nd:YAG laser at 532-nm wavelength; power 100-200 mW; retinal spot diameter 100 microm; pulse duration 100 ms). SRT lesions were applied with a Nd:YLF (527 nm; pulse duration 200 ns [30 pulses at 100 Hz]; energy 100-200 microJ/pulse; retinal spot diameter 200 microm) and were visible only angiographically. RESULTS: All CRL lesions were characterized by high reflectivity in OCT images throughout the full thickness of the neurosensory tissue 1 hr after irradiation, suggesting complete neurosensory coagulation. Strong contraction through the full thickness of the neurosensory layers was observed within 7 days after treatment. In contrast, the neural retina appeared unaffected after SRT. For both lesion types, the RPE layer appeared to be regular or thinner immediately after treatment, whereas within a period of 4 weeks, a RPE thickening indicating RPE proliferation was observable. One year and later after treatment, CRL lesions were characterized by RPE atrophy combined with significant damage of the neurosensory tissue. SRT lesions aged one year and older revealed unaffected neurosensory structures and an intact RPE layer. CONCLUSION: Spectral domain OCT can be used clinically to follow the development of laser-induced lesions over time. Postoperative RPE proliferation was observed in both CRL and SRT laser lesions. RPE atrophy appeared subsequently only in CRL lesions, whereas the neurosensory retina appeared unaffected following SRT. These results suggest the selective effect of SRT in humans without causing adverse effects to the neurosensory retina.},
       keywords = {Adult
    Aged
    Aged, 80 and over
    Atrophy
    Humans
    Laser Coagulation/*adverse effects
    Lasers, Solid-State/*adverse effects
    Middle Aged
    *Postoperative Complications
    Retina/*pathology
    Retinal Diseases/*diagnosis/*surgery
    Retinal Pigment Epithelium/pathology
    Retrospective Studies
    *Tomography, Optical Coherence},
       year = {2009}
    }
  • Huber, Robert: State-of-the-art and future of ultrahigh speed OCT. in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Huber_c,
      Title                    = {{State-of-the-art and future of ultrahigh speed OCT}},
      Author                   = {Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5191695},
      ISBN                     = {978-1-4244-4079-5},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5191695}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Huber, Robert: Recent developments in Fourier domain mode locked lasers for optical coherence tomography: imaging at 1310 nm vs. 1550 nm wavelength. Journal of biophotonics, no. 2, pp. 357-63, jul,, 2009
    BibTeX Link
    @Article{HU_2009_Biedermann_c,
      Title                    = {{Recent developments in Fourier domain mode locked lasers for optical coherence tomography: imaging at 1310 nm vs. 1550 nm wavelength}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Huber, Robert},
      Journal                  = {Journal of biophotonics},
      Year                     = {2009},
    
      Month                    = jul,
      Number                   = {6-7},
      Pages                    = {357--63},
      Volume                   = {2},
    
      Doi                      = {10.1002/jbio.200910028},
      ISSN                     = {1864-0648},
      Url                      = {http://onlinelibrary.wiley.com/doi/10.1002/jbio.200910028/abstract;jsessionid=4F65E7D7366C8202661DB8227E57EBEC.f03t04}
    }
    
  • Probst, J. and Koch, P. and Hüttmann, G: Real Time 3D Rendering of Coherence Tomography Volumetric Data. 14.-16.06.2009, 2009
    BibTeX
    @techreport{Probst,
       author = {Probst, J. and Koch, P. and Hüttmann, G},
       title = {Real Time 3D Rendering of Coherence Tomography Volumetric Data},
       month = {14.-16.06.2009},
       year = {2009}
    }
  • Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert: Raman pumped Fourier Domain Mode Locked (FDML) laser: Analysis of operation and application for optical coherence tomography (OCT). in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Klein_a,
      Title                    = {{Raman pumped Fourier Domain Mode Locked (FDML) laser: Analysis of operation and application for optical coherence tomography (OCT)}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5194704},
      ISBN                     = {978-1-4244-4079-5},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5194704}
    }
  • Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.: Optische Kohärenztomographie in der Mittelohrchirurgie. HNO, no. 57, pp. 421-427, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E. and Hüttmann, G. and Pau, H. W.},
       title = {Optische Kohärenztomographie in der Mittelohrchirurgie},
       journal = {HNO},
       volume = {57},
       number = {5},
       pages = {421-427},
       abstract = {Die Anwendung der optischen Kohärenztomographie (OCT) in der Mittelohrchirurgie gilt als vielversprechendes Diagnostikum, insbesondere wenn es um die intraoperative Abklärung der Ursache einer Stapesfixation, die intraoperative Beurteilung der Stapesfußplatte bei Revisionsstapesplastik und zur Orientierung bei der Kochleaimplantation, vorwiegend bei Missbildungen, geht. Die OCT bildet Mittel- und Innenohrstrukturen präzise ab und ermöglicht, diese zur Therapiefestlegung zu nutzen. Aus den eigenen bisherigen Ex- und In-vivo-Untersuchungen mit einem OCT-System, das an ein Operationsmikroskop gekoppelt ist, lassen sich derzeit – unter Berücksichtigung der bislang publizierten experimentellen Arbeiten – potenzielle Anwendungsgebiete definieren: Darstellung der ovalen Fensternische bei Revisionsstapes- und rekonstruktiver Mittelohrchirurgie sowie bei der explorativen Tympanotomie zur Abklärung von Perilymphfisteln und Visualisierung von Strukturen des nicht eröffneten Innenohrs.},
       keywords = {Medizin},
       year = {2009}
    }
  • Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.: Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review. Klinische Monatsblatter Fur Augenheilkunde, no. 226, pp. 958-964, 2009
    BibTeX Link
    @article{Hüttmann2009,
       author = {Huttmann, G. and Lankenau, E. and Schulz-Wackerbarth, C. and Muller, M. and Steven, P. and Birngruber, R.},
       title = {Optical Coherence Tomography: from Retina Imaging to Intraoperative Use - a Review},
       journal = {Klinische Monatsblatter Fur Augenheilkunde},
       volume = {226},
       number = {12},
       pages = {958-964},
       note = {542VT
    Times Cited:6
    Cited References Count:48},
       abstract = {Background: Optical coherence tomography (OCT) is new diagnostic procedure that has rapidly evolved in the last years. The recently developed spectral domain OCT allows one to increase the imaging speed by a hundred times compared to the first generation time domain OCT and enables three-dimensional imaging as well as real-time imaging of fast moving structures. Volumetric imaging improves the quantitative measurement of morphology and the evaluation of temporal changes. In addition, an exact correlation with images acquired with other imaging modalities is possible. Real-time imaging enables also the use of OCT during examinations with the slit-lamp and during ophthalmological surgery.
    Methods: A spectral domain OCT was adapted to a slit lamp. 70 patients (91 eyes) were examined at the anterior or posterior segment of the eye. Images of healthy structures and different pathologies were compared to OCT images obtained with Stratus 3, Spectralis, and the SL-OCT. To demonstrate the feasibility of OCT during surgery, spectral domain OCT devices working with 20000 and 210000 A scans per second were coupled by specially developed optics to the camera port of a surgical microscope. The device was tested with phantoms and enucleated pig eyes.
    Results and Conclusions: A5 kHz spectral domain OCT can image the retina during slit lamp-based indirect ophthalmoscopy with a quality similar to that of the Stratus 3. In addition, relevant structures of the anterior segment were imaged. Here, compared to commercially available devices, the imaged field was smaller and the angle of the anterior chamber was not directly visible due to the 830 nm wavelength, which had to be used for retinal imaging. Through the surgical microscope, a volumetric imaging of epithelium, Bowman's, Descemet's membranes, limbus, iris, lens, conjunctiva and sclera was demonstra-ted with several tens of centimetre working distance. Instruments and incisions in the cornea were visualised with 20 mu m precision. Real-time imaging and visualisation of volumetric OCT data were also demonstrated. In principle, all technical problems of an intraoperative use of OCT have been solved and a clinical trial will start in the near future. OCT has the potential to improve the precision of surgical interventions and may even enable new interventions.},
       keywords = {physiological optics
    cornea
    anatomy
    in-vivo
    ultrahigh-resolution
    anterior segment
    time-domain
    blood-flow
    eye
    surgery
    oct
    cyclophotocoagulation
    cornea},
       ISSN = {0023-2165},
       DOI = {DOI 10.1055/s-0028-1109939},
       url = {<Go to ISI>://WOS:000273527800005},
       year = {2009},
       type = {Journal Article}
    }
    
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Optical coherence tomography of the oval window niche.. J Laryngol Oto, no. 1, pp. 1-6, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Optical coherence tomography of the oval window niche.},
       journal = { J Laryngol Oto},
       volume = {1},
       pages = {1-6},
       year = {2009}
    }
  • Tiede, S. and Koop, N. and Kloepper, J. E. and Fassler, R. and Paus, R.: Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells. Stem Cells, no. 27, pp. 2793-803, 2009
    BibTeX Link
    @article{Tiede2009,
       author = {Tiede, S. and Koop, N. and Kloepper, J. E. and Fassler, R. and Paus, R.},
       title = {Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells},
       journal = {Stem Cells},
       volume = {27},
       number = {11},
       pages = {2793-803},
       ISSN = {1066-5099},
       DOI = {10.1002/stem.213},
       year = {2009},
       type = {Journal Article}
    }
    
  • Leonhardt, Rainer and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert: Nonlinear optical frequency conversion of an amplified Fourier Domain Mode Locked (FDML) laser.. Optics express, no. 17, pp. 16801-8, OSA, sep,, 2009
    BibTeX Link
    @Article{HU_2009_Leonhardt_a,
      Title                    = {{Nonlinear optical frequency conversion of an amplified Fourier Domain Mode Locked (FDML) laser.}},
      Author                   = {Leonhardt, Rainer and Biedermann, Benjamin R and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = sep,
      Number                   = {19},
      Pages                    = {16801--8},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.016801},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=oe-17-19-16801}
    }
  • Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.: Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density. Acta Neurochir, no. 151, pp. 253-262, 2009
    BibTeX
    @article{Kandelhardt,
       author = {Kandelhardt, S.R. and Leppert, J. and Kandelhardt, JW and Reusche, E. and Hüttmann, G and Giese, A.},
       title = {Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density},
       journal = {Acta Neurochir },
       volume = {151},
       pages = {253-262},
       year = {2009}
    }
  • Freidank, S. and Linz, N.: Mit der biomedizinischen Optik hoch hinaus - Lübecker Projekt zur Laserforschung auf der Zugspitze. Focus Uni Luebeck / Universität Lübeck, no. 26, pp. 16, 2009
    BibTeX
    @article{Freidank,
       author = {Freidank, S. and Linz, N.},
       title = {Mit der biomedizinischen Optik hoch hinaus - Lübecker Projekt zur Laserforschung auf der Zugspitze },
       journal = {Focus Uni Luebeck / Universität Lübeck},
       volume = {26},
       number = {1},
       pages = {16},
       year = {2009}
    }
  • Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.: Intra-operative application of optical coherence tomography with an operating microscope.. J Laryngol Otol, no. 123, pp. 1027-1030, 2009
    BibTeX
    @article{Just,
       author = {Just, T. and Lankenau, E and Hüttmann, G and Pau, H.W.},
       title = {Intra-operative application of optical coherence tomography with an operating microscope.},
       journal = {J Laryngol Otol},
       volume = {123},
       pages = {1027-1030},
       year = {2009}
    }
  • Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.: Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection. J Biomed Opt, no. 14, pp. 054034, 2009
    BibTeX
    @article{Yao,
       author = {Yao, C and Qu, X. and Zhang, Z. and B., Yao and Hüttmann, G and Rahmanzadeh, R.},
       title = {Influence of Laser Parameters on Membrane Permeability with Nanoparticles and Targeted Antibody Transfection},
       journal = {J Biomed Opt},
       volume = {14},
       pages = {054034},
       note = {Journal article},
       year = {2009}
    }
  • Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.: Imaging of human brain tumor tissue by near-infrared laser coherence tomography. Acta Neurochir (Wien), no. 151, pp. 507-17; discussion 517, 2009
    BibTeX
    @article{Böhringer,
       author = {Böhringer, H. J. and Lankenau, E. and Stellmacher, F. and Reusche, E. and Huttmann, G. and Giese, A.},
       title = {Imaging of human brain tumor tissue by near-infrared laser coherence tomography},
       journal = {Acta Neurochir (Wien)},
       volume = {151},
       number = {5},
       pages = {507-17; discussion 517},
       note = {Bohringer, H J
    Lankenau, E
    Stellmacher, F
    Reusche, E
    Huttmann, G
    Giese, A
    Austria
    Acta Neurochir (Wien). 2009 May;151(5):507-17; discussion 517. Epub 2009 Apr 3.},
       abstract = {INTRODUCTION: Intraoperative detection of residual tumor remains an important challenge in surgery to treat gliomas. New developments in optical techniques offer non-invasive high-resolution imaging that may integrate well into the workflow of neurosurgical operations. Using an intracranial glioma model, we have recently shown that time domain optical coherence tomography (OCT) allows discrimination of normal brain, diffusely invaded brain tissue, and solid tumor. OCT imaging allowed acquisition of 2D and 3D data arrays for multiplanar analysis of the tumor to brain interface. In this study we have analyzed biopsy specimens of human brain tumors and we present the first feasibility study of intraoperative OCT and post-image acquisition processing for non-invasive imaging of the brain and brain tumor. METHODS: We used a Sirius 713 Tomograph with a superluminescence diode emitting light at a near infrared central wavelength of 1,310 nm and a coherence length of 15 microm. The light is passed through an optical mono mode fiber to a modified OCT adapter containing a lens system with a working distance of 10 cm and an integrated pilot laser. Navigation-registered tumor biopsies were imaged ex vivo and the intraoperative site of optical tissue analysis was registered by marker acquisition using a neuronavigation system. RESULTS: Optical coherence tomography non-contact measurements of brain and brain tumor tissue produced B-scan images of 4 mm in width and 1.5-2.0 mm in depth at an axial and lateral optical resolution of 15 microm. OCT imaging demonstrated a different microstructure and characteristic signal attenuation profiles of tumor versus normal brain. Post-image acquisition processing and automated detection of the tissue to air interface was used to realign A-scans to compensate for image distortions caused by pulse- and respiration-induced movements of the target volume. Realigned images allowed monitoring of intensity changes within the scan line and facilitated selection of areas for the averaging of A-scans and the calculation of attenuation coefficients for specific regions of interest. CONCLUSION: This feasibility study has demonstrated that OCT analysis of the tissue microstructure and light attenuation characteristics discriminate normal brain, areas of tumor infiltrated brain, solid tumor, and necrosis. The working distance of the OCT adapter and the A-scan acquisition rate conceptually allows integration of the OCT applicator into the optical path of the operating microscopes. This would allow a continuous analysis of the resection plain, providing optical tomography, thereby adding a third dimension to the microscopic view and information on the light attenuation characteristics of the tissue.},
       keywords = {Biopsy
    Brain Neoplasms/*diagnosis/pathology/surgery
    Feasibility Studies
    Glioma/*diagnosis/pathology/surgery
    Humans
    Monitoring, Intraoperative/methods
    Pilot Projects
    Tomography, Optical Coherence/*methods},
       year = {2009}
    }
  • Huber, Robert: Fourier domain mode locking: new lasers for optical coherence tomography. in SPIE Newsroom, 2009
    BibTeX Link
    @Misc{HU_2009_Huber_b,
      Title                    = {{Fourier domain mode locking: new lasers for optical coherence tomography}},
    
      Author                   = {Huber, Robert},
      Year                     = {2009},
    
      Booktitle                = {SPIE Newsroom},
      Doi                      = {10.1117/2.1200901.1440},
      ISSN                     = {18182259},
      Url                      = {http://www.spie.org/x33321.xml}
    }
  • Palte, Gesa and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Huber, Robert: Fourier domain mode locked (FDML) lasers for polarization sensitive OCT. in European Conferences on Biomedical Optics, pp. 73720M-73720M-6, International Society for Optics and Photonics, jul,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Palte_a,
      Title                    = {{Fourier domain mode locked (FDML) lasers for polarization sensitive OCT}},
      Author                   = {Palte, Gesa and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Huber, Robert},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2009},
      Editor                   = {Andersen, Peter E and Bouma, Brett E},
      Month                    = jul,
      Pages                    = {73720M--73720M--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.831835},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1339476}
    }
  • Framme, C. and Panagakis, G. and Birngruber, R.: Effects on Choroidal Neovascularizations after Anti-VEGF Upload Using Intravitreal Ranibizumab as Determined by Spectral Domain Optical Coherence Tomography. Invest Ophthalmol Vis Sci, 2009
    BibTeX Link
    @article{Framme2009,
       author = {Framme, C. and Panagakis, G. and Birngruber, R.},
       title = {Effects on Choroidal Neovascularizations after Anti-VEGF Upload Using Intravitreal Ranibizumab as Determined by Spectral Domain Optical Coherence Tomography},
       journal = {Invest Ophthalmol Vis Sci},
       note = {Journal article
    Investigative ophthalmology & visual science
    Invest Ophthalmol Vis Sci. 2009 Oct 29.},
       abstract = {Purpose: To image CNV structure before and after anti-VEGF treatment using spectral domain optical coherence tomography (SD-OCT). Material and methods: In 78 patients newly diagnosed CNVs (classic CNV: n=16; occult CNV: n=54; minimal classic CNV: n=8) due to AMD were imaged before and 4 weeks after anti-VEGF upload with 3 intravitreal injections of Ranibizumab. Qualitative (structural changes) and quantitative measurements (diameter and thickness) of the CNVs were obtained from the OCT images using the Heidelberg Eye Explorer software. Results: Classic CNV components were observed above the RPE/photoreceptor complex, whereas occult CNVs stayed below. Qualitatively, overall CNV architecture appeared to be unchanged in 78%, reduced in thickness in 18%, and became larger in 4%. Quantitatively, for all CNV subtypes the diameter of CNVs (preoperatively: 2813mum; postoperatively: 2804mum) did not change after treatment (classic CNVs: p=0.390; occult CNVs: p=0.405, minimal classic CNVs: p=0.092) independently of postoperative retinal edema. The overall thickness of the CNV; however, reduced from 205mum to 175mum (p=0,000). Thickness reduction was significantly enhanced especially in CNVs with classic components (n=24; 252mum to 197mum (p=0.000); reduction: 22%), whereas reduction was smaller but also significant in occult CNVs (183mum to 164mum (p=0.003); reduction: 10%). Conclusion: Using SD-OCT CNV size can be two-dimensionally determined and followed up after intravitreal anti-VEGF treatment. Quantitative measurements underlined stable CNV diameters for all subtypes but revealed significant reduction of thickness especially for classic CNV components. Thus, Ranibizumab monotherapy is able to morphologically stop further CNV growth but does not lead to a major regression of CNVs especially of its occult components.},
       ISSN = {1552-5783 (Electronic)
    0146-0404 (Linking)},
       DOI = {iovs.09-4496 [pii]
    10.1167/iovs.09-4496},
       url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19875667},
       year = {2009},
       type = {Journal Article}
    }
    
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Dispersion, coherence and noise of Fourier domain mode locked lasers. Optics Express, no. 17, pp. 9947 - 9961, may,, 2009
    BibTeX Link
    @Article{HU_2009_Biedermann_a,
      Title                    = {{Dispersion, coherence and noise of Fourier domain mode locked lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Journal                  = {Optics Express},
      Year                     = {2009},
    
      Month                    = may,
      Number                   = {12},
      Pages                    = {9947 -- 9961 },
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.009947},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik, Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen, Munich, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-12-9947}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert: Dispersion, coherence and noise of Fourier Domain Mode Locked (FDML) lasers. in CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, pp. 1-1, IEEE, jun,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Biedermann_b,
      Title                    = {{Dispersion, coherence and noise of Fourier Domain Mode Locked (FDML) lasers}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Klein, Thomas and Huber, Robert},
      Booktitle                = {CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference},
      Year                     = {2009},
      Month                    = jun,
      Pages                    = {1--1},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/CLEOE-EQEC.2009.5192900},
      ISBN                     = {978-1-4244-4079-5},
      Pmid                     = {19506645},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5192900}
    }
  • Lachmann, Kristina and Eckert, Sebastian and Vogel, Alfred and Klinger, Antje and Gebert, Andreas and Klages, Claus-Peter: Development of a multifunctional coating system for laser-induced material transport. Progress in Organic Coatings, no. 64, pp. 294-299, 2009
    BibTeX
    @article{Lachmann,
       author = {Lachmann, Kristina and Eckert, Sebastian and Vogel, Alfred and Klinger, Antje and Gebert, Andreas and Klages, Claus-Peter},
       title = {Development of a multifunctional coating system for laser-induced material transport},
       journal = {Progress in Organic Coatings},
       volume = {64},
       number = {2–3},
       pages = {294-299},
       abstract = {The aim of our research is to develop a novel surface coating for the use in laser microdissection and laser pressure catapulting (LMPC). LMPC is a contact- and contamination-free technique to separate histologic material and living cells for further proteomic and genomic analysis. Several physico-chemical functions must be included within the optimum coating system designed for this purpose, like optical absorption at the laser wavelength, combined with optical transparency in the visible region, a control of the laser ablation process, mechanical stability and biocompability for the adhesion of the histologic material. To achieve the optimum system the combination of several layers is required. The optical absorbance to capture the radiation energy from a frequency-tripled Nd:YAG laser (?&#xa0;=&#xa0;355&#xa0;nm) is reached by a thin layer of zinc oxide (ZnO), deposited by hollow cathode gas flow sputtering. The laser ablation process is controlled by a polyelectrolyte multilayer, consisting of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS). The evaporation of chemisorbed water from the film is used to promote the catapulting process. For the mechanically stable, laser-dissectible layer organic coatings, like photoresists or lacquers, are suitable. Silica-containing polyacrylate nanocomposites were employed for this purpose. The investigation of the coating system included LMPC experiments with varying compositions of the layer system. The best results were obtained using a system consisting of ZnO, a polyelectrolyte multilayer deposited from 0.1&#xa0;M Na2SO4 containing polymer solutions, and a 1.5-?m thick layer of the polyacrylate nanocomposite. To check the quality of the developed system, experiments with the commonly used poly(ethylene naphthalate) (PEN) foil were performed simultaneously. In addition to the determination of the parameters required for LMPC, quantitative real-time Polymerase Chain Reaction (rt-PCR) of the dissected material verified the benefit of the new system.},
       keywords = {Laser microdissection
    Laser pressure catapulting
    Polyelectrolyte multilayer
    Laser ablation
    Zinc oxide},
       year = {2009}
    }
  • Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon: Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies. Journal of Biomedical Optics, no. 14, pp. 064040-064040, 2009
    BibTeX
    @article{Steven,
       author = {Steven, Philipp and Müller, Maya and Koop, Norbert and Rose, Christian and Hüttmann, Gereon},
       title = {Comparison of Cornea Module and DermaInspect for noninvasive imaging of ocular surface pathologies},
       journal = {Journal of Biomedical Optics},
       volume = {14},
       number = {6},
       pages = {064040-064040},
       note = {10.1117/1.3275475},
       abstract = {Minimally invasive imaging of ocular surface pathologies aims at securing clinical diagnosis without actual tissue probing. For this matter, confocal microscopy (Cornea Module) is in daily use in ophthalmic practice. Multiphoton microscopy is a new optical technique that enables high-resolution imaging and functional analysis of living tissues based on tissue autofluorescence. This study was set up to compare the potential of a multiphoton microscope (DermaInspect) to the Cornea Module. Ocular surface pathologies such as pterygia, papillomae, and nevi were investigated in vivo using the Cornea Module and imaged immediately after excision by DermaInspect. Two excitation wavelengths, fluorescence lifetime imaging and second-harmonic generation (SHG), were used to discriminate different tissue structures. Images were compared with the histopathological assessment of the samples. At wavelengths of 730nm, multiphoton microscopy exclusively revealed cellular structures. Collagen fibrils were specifically demonstrated by second-harmonic generation. Measurements of fluorescent lifetimes enabled the highly specific detection of goblet cells, erythrocytes, and nevus-cell clusters. At the settings used, DermaInspect reaches higher resolutions than the Cornea Module and obtains additional structural information. The parallel detection of multiphoton excited autofluorescence and confocal imaging could expand the possibilities of minimally invasive investigation of the ocular surface toward functional analysis at higher resolutions.},
       year = {2009}
    }
  • Bever, M. and Schlott, K. and Ptaszynski, L. and Koinzer, S. and Birngruber, R. and Brinkmann, R.: Automatische Dosimetrie bei der Laserphotokoagulation der Netzhaut. 3. Dresdner Medizintechnik-Symposium mit DFG Forschungsschwerpunkt Protektive Beatmungskonzepte, no. 10, 2009
    BibTeX
    @article{Bever2009,
       author = {Bever, M. and Schlott, K. and Ptaszynski, L. and Koinzer, S. and Birngruber, R. and Brinkmann, R.},
       title = {Automatische Dosimetrie bei der Laserphotokoagulation der Netzhaut},
       journal = {3. Dresdner Medizintechnik-Symposium mit DFG Forschungsschwerpunkt Protektive Beatmungskonzepte},
       volume = {10},
       year = {2009},
       type = {Journal Article}
    }
    
  • Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.: Automated segmentation of tissue structures in optical coherence tomography data. J Biomed Opt, no. 14, pp. 034046, 2009
    BibTeX
    @article{Gasca,
       author = {Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.},
       title = {Automated segmentation of tissue structures in optical coherence tomography data},
       journal = {J Biomed Opt},
       volume = {14},
       number = {3},
       pages = {034046},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Segmentation of optical coherence tomography (OCT) images provides useful information, especially in medical imaging applications. Because OCT images are subject to speckle noise, the identification of structures is complicated. Addressing this issue, two methods for the automated segmentation of arbitrary structures in OCT images are proposed. The methods perform a seeded region growing, applying a model-based analysis of OCT A-scans for the seed's acquisition. The segmentation therefore avoids any user-intervention dependency. The first region-growing algorithm uses an adaptive neighborhood homogeneity criterion based on a model of an OCT intensity course in tissue and a model of speckle noise corruption. It can be applied to an unfiltered OCT image. The second performs region growing on a filtered OCT image applying the local median as a measure for homogeneity in the region. Performance is compared through the quantitative evaluation of artificial data, showing the capabilities of both in terms of structures detected and leakage. The proposed methods were tested on real OCT data in different scenarios and showed promising results for their application in OCT imaging.},
       year = {2009}
    }
  • Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.: Automated segmentation of tissue structures in optical coherence tomography data. J Biomed Opt, no. 14, pp. 034046, 2009
    BibTeX
    @article{Gasca,
       author = {Gasca, F. and Ramrath, L. and Huettmann, G. and Schweikard, A.},
       title = {Automated segmentation of tissue structures in optical coherence tomography data},
       journal = {J Biomed Opt},
       volume = {14},
       number = {3},
       pages = {034046},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Segmentation of optical coherence tomography (OCT) images provides useful information, especially in medical imaging applications. Because OCT images are subject to speckle noise, the identification of structures is complicated. Addressing this issue, two methods for the automated segmentation of arbitrary structures in OCT images are proposed. The methods perform a seeded region growing, applying a model-based analysis of OCT A-scans for the seed's acquisition. The segmentation therefore avoids any user-intervention dependency. The first region-growing algorithm uses an adaptive neighborhood homogeneity criterion based on a model of an OCT intensity course in tissue and a model of speckle noise corruption. It can be applied to an unfiltered OCT image. The second performs region growing on a filtered OCT image applying the local median as a measure for homogeneity in the region. Performance is compared through the quantitative evaluation of artificial data, showing the capabilities of both in terms of structures detected and leakage. The proposed methods were tested on real OCT data in different scenarios and showed promising results for their application in OCT imaging.},
       year = {2009}
    }
  • Huber, Robert: Advances in Fourier domain OCT. in 2009 IEEE LEOS Annual Meeting Conference Proceedings, pp. 201-202, IEEE, oct,, 2009
    BibTeX Link
    @InProceedings{HU_2009_Huber_a,
      Title                    = {{Advances in Fourier domain OCT}},
      Author                   = {Huber, Robert},
      Booktitle                = {2009 IEEE LEOS Annual Meeting Conference Proceedings},
      Year                     = {2009},
      Month                    = oct,
      Pages                    = {201--202},
      Publisher                = {IEEE},
    
      Doi                      = {10.1109/LEOS.2009.5343314},
      ISBN                     = {978-1-4244-3680-4},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=5343314}
    }
    
  • Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert: A theoretical description of Fourier domain mode locked lasers. Optics express, no. 17, pp. 24013-9, dec,, 2009
    BibTeX Link
    @Article{HU_2009_Jirauschek_a,
      Title                    = {{A theoretical description of Fourier domain mode locked lasers}},
      Author                   = {Jirauschek, Christian and Biedermann, Benjamin and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = dec,
      Number                   = {26},
      Pages                    = {24013--9},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.024013},
      Institution              = {Institute for Nanoelectronics, Technische Universit\"{a}t M\"{u}nchen, Arcisstrasse 21, D-80333 Munich, Germany. jirauschek@tum.de},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-26-24013}
    }
  • Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P: A 75 MHz light source for femtosecond stimulated raman microscopy. Optics express, no. 17, pp. 18612-20, oct,, 2009
    BibTeX Link
    @Article{HU_2009_Ploetz_a,
      Title                    = {{A 75 MHz light source for femtosecond stimulated raman microscopy}},
      Author                   = {Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {18612--20},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.018612},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik and Center of Integrated Protein Science Munich Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen Oettingenstr 67, D-80538 M\"{u}nchen, Germany.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-21-18612}
    }