2008 - 2007

2008

  • Srinivasan, Vivek J and Adler, Desmond C and Chen, Yueli and Gorczynska, Iwona and Huber, Robert and Duker, Jay S and Schuman, Joel S and Fujimoto, James G: Ultrahigh-speed optical coherence tomography for three-dimensional and en face imaging of the retina and optic nerve head.. Investigative ophthalmology & visual science, no. 49, pp. 5103-10, nov,, 2008
    BibTeX Link
    @Article{HU_2008_Srinivasan_a,
      Title                    = {{Ultrahigh-speed optical coherence tomography for three-dimensional and en face imaging of the retina and optic nerve head.}},
      Author                   = {Srinivasan, Vivek J and Adler, Desmond C and Chen, Yueli and Gorczynska, Iwona and Huber, Robert and Duker, Jay S and Schuman, Joel S and Fujimoto, James G},
      Journal                  = {Investigative ophthalmology \& visual science},
      Year                     = {2008},
    
      Month                    = nov,
      Number                   = {11},
      Pages                    = {5103--10},
      Volume                   = {49},
    
      Doi                      = {10.1167/iovs.08-2127},
      ISSN                     = {1552-5783},
      Url                      = {http://www.iovs.org/content/49/11/5103}
    }
  • Ramrath, L. and Moreno, G. and Mueller, H. and Bonin, T. and Huettmann, G. and Schweikard, A.: Towards multi-directional OCT for speckle noise reduction. Med Image Comput Comput Assist Interv, no. 11, pp. 815-23, 2008
    BibTeX
    @article{Ramrath,
       author = {Ramrath, L. and Moreno, G. and Mueller, H. and Bonin, T. and Huettmann, G. and Schweikard, A.},
       title = {Towards multi-directional OCT for speckle noise reduction},
       journal = {Med Image Comput Comput Assist Interv},
       volume = {11},
       number = {Pt 1},
       pages = {815-23},
       note = {Using Smart Source Parsing},
       abstract = {Multi-directional optical coherence tomography (MD-OCT) applies and extends the concept of angular compounding for speckle noise reduction to the area of OCT imaging. OCT images are acquired from a wide range of angles of view. Averaging of the rotated images therefore requires compensation of the parallax which is achieved by simple image registration for image reconstruction. Test measurements of a sample structure in a low and highly scattering environment show that the method improves the signal-to-noise ratio by a factor of 4 and hence reduces speckle noise significantly. Experimental results also show that the proposed averaging increases the performance of common edge-detection algorithms.},
       year = {2008}
    }
  • Neumann, J and Brinkmann, R: Self-limited growth of laser-induced vapor bubbles around single micro-absorbers. Appl Phys Lett, no. 93, pp. 033901, 2008
    BibTeX
    @article{Neumann,
       author = {Neumann, J and Brinkmann, R},
       title = {Self-limited growth of laser-induced vapor bubbles around single micro-absorbers},
       journal = {Appl  Phys Lett},
       volume = {93},
       pages = {033901},
       year = {2008}
    }
  • Koinzer, S and Elsner, H and Klatt, C and Brinkmann, R and Birngruber, R and Roider, J: Selective Retina Therapy (SRT) of chronic subfoveal fluid after surgery of rhegmatogenous retinal detachment: three case reports. no. 246, pp. 1373-8, 2008
    BibTeX
    @misc{Koinzer,
       author = {Koinzer, S and Elsner, H and Klatt, C and Brinkmann, R and Birngruber, R and Roider, J},
       title = {Selective Retina Therapy (SRT) of chronic subfoveal fluid after surgery of rhegmatogenous retinal detachment: three case reports},
       volume = {246},
       number = {10},
       pages = {1373-8},
       year = {2008}
    }
  • Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Palte, Gesa and Adler, Desmond C and Srinivasan, Vivek J and Fujimoto, James G and Huber, Robert: Real time en face Fourier-domain optical coherence tomography with direct hardware frequency demodulation. Optics Letters, no. 33, pp. 2556 - 2558, oct,, 2008
    BibTeX Link
    @Article{HU_2008_Biedermann_a,
      Title                    = {{Real time en face Fourier-domain optical coherence tomography with direct hardware frequency demodulation}},
      Author                   = {Biedermann, Benjamin R and Wieser, Wolfgang and Eigenwillig, Christoph M and Palte, Gesa and Adler, Desmond C and Srinivasan, Vivek J and Fujimoto, James G and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2008},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {2556 -- 2558 },
      Volume                   = {33},
    
      Doi                      = {10.1364/OL.33.002556},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-33-21-2556}
    }
  • Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert: Raman-pumped Fourier-domain mode-locked laser: analysis of operation and application for optical coherence tomography. Optics Letters, no. 33, pp. 2815 - 2817, OSA, nov,, 2008
    BibTeX Link
    @Article{HU_2008_Klein_a,
      Title                    = {{Raman-pumped Fourier-domain mode-locked laser: analysis of operation and application for optical coherence tomography}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Eigenwillig, Christoph M and Palte, Gesa and Huber, Robert},
      Journal                  = {Optics Letters},
      Year                     = {2008},
    
      Month                    = nov,
      Number                   = {23},
      Pages                    = {2815 -- 2817 },
      Volume                   = {33},
    
      Doi                      = {10.1364/OL.33.002815},
      ISSN                     = {0146-9592},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-33-23-2815}
    }
  • Adler, Desmond C and Huang, Shu-Wei and Huber, Robert and Fujimoto, James G: Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography. Optics Express, no. 16, pp. 4376 - 4393, mar,, 2008
    BibTeX Link
    @Article{HU_2008_Adler_a,
      Title                    = {{Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography}},
      Author                   = {Adler, Desmond C and Huang, Shu-Wei and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = mar,
      Number                   = {7},
      Pages                    = {4376 -- 4393 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.004376},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-7-4376}
    }
  • Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W: Optically controlled thermal management on the nanometer length scale. Nanotechnology, no. 19, pp. 055207, 2008
    BibTeX
    @article{Garwe,
       author = {Garwe, F and Bauerschäfer, U and Csaki, A and Steinbrück, A and Ritter, K and Bochmann, A and Bergmann, J and Weise, A and Akimov, D and Maubach, G and König, K and Hüttmann, G and Paa, W and Popp, J and Fritzsche, W},
       title = {Optically controlled thermal management on the nanometer length scale},
       journal = {Nanotechnology},
       volume = {19},
       number = {5},
       pages = {055207},
       abstract = {The manipulation of polymers and biological molecules or the control of chemical reactions on a nanometer scale by means of laser pulses shows great promise for applications in modern nanotechnology, biotechnology, molecular medicine or chemistry. A controllable, parallel, highly efficient and very local heat conversion of the incident laser light into metal nanoparticles without ablation or fragmentation provides the means for a tool like a 'nanoreactor', a 'nanowelder', a 'nanocrystallizer' or a 'nanodesorber'. In this paper we explain theoretically and show experimentally the interaction of laser radiation with gold nanoparticles on a polymethylmethacrylate (PMMA) layer (one-photon excitation) by means of different laser pulse lengths, wavelengths and pulse repetition rates. To the best of our knowledge this is the first report showing the possibility of highly local (in a 40 nm range) regulated heat insertion into the nanoparticle and its surroundings without ablation of the gold nanoparticles. In an earlier paper we showed that near-infrared femtosecond irradiation can cut labeled DNA sequences in metaphase chromosomes below the diffraction-limited spot size. Now, we use gold as well as silver-enhanced gold nanoparticles on DNA (also within chromosomes) as energy coupling objects for femtosecond laser irradiation with single-and two-photon excitation. We show the results of highly localized destruction effects on DNA that occur only nearby the nanoparticles.},
       year = {2008}
    }
  • Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert: Optical coherence tomography imaging with k-space linear Fourier Domain Mode Locked lasers. in 2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS, 2008
    BibTeX Link
    @InProceedings{HU_2008_Eigenwillig_a,
      Title                    = {{Optical coherence tomography imaging with k-space linear Fourier Domain Mode Locked lasers}},
      Author                   = {Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert},
      Booktitle                = {2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS},
      Year                     = {2008},
    
      Url                      = {http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=4571261&searchWithin%3Deigenwillig%26sortType%3Dasc_p_Sequence%26filter%3DAND%28p_IS_Number%3A4571172%29}
    }
  • Sandeau, J. and Caillibotte, G. and Kandulla, J. and Elsner, H. and Brinkmann, R and Birngruber, R. and Apiou-Sbirlea, G.: Numerical Modelling of Conductive and Convective Heat Transfers in Retinal Laser Applications. no. 1, pp. 43-52, 2008
    BibTeX
    @misc{Sandeau,
       author = {Sandeau, J. and Caillibotte, G. and Kandulla, J. and Elsner, H. and Brinkmann, R and Birngruber, R. and Apiou-Sbirlea, G.},
       title = {Numerical Modelling of Conductive and Convective Heat Transfers in Retinal  Laser Applications},
       volume = {1},
       number = {1},
       pages = {43-52},
       year = {2008}
    }
  • Kandulla, J and Brinkmann, R: Non invasive real-time temperature determination during laser treatments at the retina. Photonik international, no. 2008/1, pp. 42-45, 2008
    BibTeX
    @article{Kandulla,
       author = {Kandulla, J and Brinkmann, R},
       title = {Non invasive real-time temperature determination during laser treatments at the retina},
       journal = {Photonik international},
       volume = {2008/1},
       pages = {42-45},
       year = {2008}
    }
  • Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.: Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental. Laser Surg Med, no. 40, pp. 273-81, 2008
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S.R. and Diddens, H. and Leppert, J. and Rohde, V. and Hüttmann, G. and Giese, A.},
       title = {Multiphoton excited fluorescence microscopy of 5-ALA induced fluorescence in exprimental},
       journal = {Laser Surg Med},
       volume = {40
    },
       number = {4},
       pages = {273-81},
       year = {2008}
    }
  • Mueller, M. and Huettmann, G. and Koop, N. and Steven, P.: Minimal-Invasive Imaging of Ocular Surface Pathologies - Confocal vs. Two-Photon Microscopy. Investigative Ophthalmology & Visual Science, no. 49, pp. 2258-2258, 2008
    BibTeX Link
    @article{Mueller2008,
       author = {Mueller, M. and Huettmann, G. and Koop, N. and Steven, P.},
       title = {Minimal-Invasive Imaging of Ocular Surface Pathologies - Confocal vs. Two-Photon Microscopy},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {49},
       number = {13},
       pages = {2258-2258},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2008},
       type = {Journal Article}
    }
    
  • Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.: Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests. in Plant J, no. 54, pp. 938-48, 2008
    BibTeX
    @incollection{Phillips,
       author = {Phillips, J. R. and Fischer, E. and Baron, M. and van den Dries, N. and Facchinelli, F. and Kutzer, M. and Rahmanzadeh, R. and Remus, D. and Bartels, D.},
       title = {Lindernia brevidens: a novel desiccation-tolerant vascular plant, endemic to ancient tropical rainforests},
       booktitle = {Plant J},
       volume = {54},
       edition = {2008/03/19},
       pages = {938-48},
       note = {Phillips, Jonathan R
    Fischer, Eberhard
    Baron, Miriam
    van den Dries, Niels
    Facchinelli, Fabio
    Kutzer, Michael
    Rahmanzadeh, Ramtin
    Remus, Daniela
    Bartels, Dorothea
    England
    Plant J. 2008 Jun;54(5):938-48. Epub 2008 Mar 13.},
       abstract = {A particular adaptation to survival under limited water availability has been realized in the desiccation-tolerant resurrection plants, which tend to grow in a habitat with seasonal rainfall and long dry periods. One of the best-studied examples is Craterostigma plantagineum. Here we report an unexpected finding: Lindernia brevidens, a close relative of C. plantagineum, exhibits desiccation tolerance, even though it is endemic to the montane rainforests of Tanzania and Kenya, where it never experiences seasonal dry periods. L. brevidens has been found exclusively in two fragments of the ancient Eastern Arc Mountains, which were protected from the devastating Pleistocene droughts by the stable Indian Ocean temperature. Analysis of the microhabitat reveals that L. brevidens is found in the same habitat as hygrophilous plant species, which further indicates that the plant never dries out completely. The objective of this investigation was to address whether C. plantagineum and L. brevidens have desiccation-related pathways in common, or whether L. brevidens has acquired novel pathways. A third, closely related, desiccation-sensitive species, Lindernia subracemosa, has been included for comparison. Mechanisms that confer cellular protection during extreme water loss are well conserved between C. plantagineum and L. brevidens, including the interconversion of 2-octulose to sucrose within the two desiccation-tolerant species. Furthermore, transcriptional control regions of desiccation-related genes belonging to the late embryogenesis abundant (LEA) protein family are also highly conserved. We propose that L. brevidens is a neoendemic species that has retained desiccation tolerance through genome stability, despite tolerance being superfluous to environmental conditions.},
       keywords = {Adaptation, Physiological
    Gene Expression Profiling
    Genome, Plant
    Lamiaceae/genetics/metabolism/ physiology
    Sucrose/metabolism
    Tropical Climate
    Water},
       year = {2008}
    }
  • Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.: Laser-based gene transfection and gene therapy. IEEE Trans Nanobioscience, no. 7, pp. 111-9, 2008
    BibTeX
    @article{Yao,
       author = {Yao, C. P. and Zhang, Z. X. and Rahmanzadeh, R. and Huettmann, G.},
       title = {Laser-based gene transfection and gene therapy},
       journal = {IEEE Trans Nanobioscience},
       volume = {7},
       number = {2},
       pages = {111-9},
       note = {Yao, C P
    Zhang, Z X
    Rahmanzadeh, R
    Huettmann, G
    Research Support, Non-U.S. Gov't
    Review
    United States
    IEEE Trans Nanobioscience. 2008 Jun;7(2):111-9.},
       abstract = {The plasma membrane of mammalian cells can be transiently permeablized by optical means and exogenous materials or genes can be introduced into the cytoplasm of living cells. Until now, few mechanisms were exploited for the manipulation: laser is directly and tightly focused on the cells for optoinjection, laser-induced stress waves, photochemical internalization, and irradiation of selective cell targeting with light-absorbing particles. During the past few years, extensive progress and numerous breakthroughs have been made in this area of research. This review covers four different laser-assisted transfection techniques and their advantages and disadvantages. Universality towards various cell lines is possibly the main advantage of laser-assisted optoporation in comparison with presently existing methods of cell transfection.},
       keywords = {Cell Membrane/ radiation effects
    DNA/ administration & dosage/ pharmacokinetics
    Gene Therapy/ methods
    Lasers
    Transfection/ methods},
       year = {2008}
    }
  • Eigenwillig, Christoph M and Biedermann, Benjamin R and Palte, Gesa and Huber, Robert: K-space linear Fourier domain mode locked laser and applications for optical coherence tomography. Optics Express, no. 16, pp. 8916 - 8937, OSA, jun,, 2008
    BibTeX Link
    @Article{HU_2008_Eigenwillig_b,
      Title                    = {{K-space linear Fourier domain mode locked laser and applications for optical coherence tomography}},
      Author                   = {Eigenwillig, Christoph M and Biedermann, Benjamin R and Palte, Gesa and Huber, Robert},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = jun,
      Number                   = {12},
      Pages                    = {8916 -- 8937 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.008916},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
      Url                      = {http://www.opticsexpress.org/abstract.cfm?URI=oe-16-12-8916}
    }
  • Framme, C and Schüle, G and Kobuch, K and Flucke, B and Birngruber, R and Brinkmann, R: Investigation of Selective Retina Treatment (SRT) by Means of 8 ns Laser Pulses in a Rabbit Model. no. 40, pp. 20-27, 2008
    BibTeX
    @misc{Framme,
       author = {Framme, C and Schüle, G and Kobuch, K and Flucke, B and Birngruber, R and Brinkmann, R},
       title = {Investigation of Selective Retina Treatment (SRT) by Means of 8 ns Laser Pulses in a Rabbit Model},
       volume = {40},
       pages = {20-27},
       year = {2008}
    }
  • Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G: In vivo endomicroscopy using three-dimensional optical coherence tomography and Fourier domain mode locked lasers. in Biomedical Optics (BiOS) 2008, pp. 684708-684708-7, International Society for Optics and Photonics, feb,, 2008
    BibTeX Link
    @InProceedings{HU_2008_Adler_b,
      Title                    = {{In vivo endomicroscopy using three-dimensional optical coherence tomography and Fourier domain mode locked lasers}},
      Author                   = {Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G},
      Booktitle                = {Biomedical Optics (BiOS) 2008},
      Year                     = {2008},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {684708--684708--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.761850},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1326810}
    }
    
  • Bajraszewski, Tomasz and Wojtkowski, Maciej and Szkulmowski, Maciej and Szkulmowska, Anna and Huber, Robert and Kowalczyk, Andrzej: Improved spectral optical coherence tomography using optical frequency comb. Optics Express, no. 16, pp. 4163 - 4176, mar,, 2008
    BibTeX Link
    @Article{HU_2008_Bajraszewski_a,
      Title                    = {{Improved spectral optical coherence tomography using optical frequency comb}},
      Author                   = {Bajraszewski, Tomasz and Wojtkowski, Maciej and Szkulmowski, Maciej and Szkulmowska, Anna and Huber, Robert and Kowalczyk, Andrzej},
      Journal                  = {Optics Express},
      Year                     = {2008},
    
      Month                    = mar,
      Number                   = {6},
      Pages                    = {4163 -- 4176 },
      Volume                   = {16},
    
      Doi                      = {10.1364/OE.16.004163},
      Institution              = {Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5/7, 87-100 Torun, Poland.},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-16-6-4163}
    }
  • Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.: Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes. no. 13, pp. 031217, 2008
    BibTeX
    @misc{Qu,
       author = {Qu, X. and Wang, J. and Zhang, Z. and Koop, N. and Rahmanzadeh, R. and Huttmann, G.},
       title = {Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes},
       volume = {13},
       number = {3},
       pages = {031217},
       note = {Using Smart Source Parsing
    May-Jun},
       abstract = {Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas-299). From the FLIM images, we can easily discriminate the imaging difference between cells and optical probes according to their distinct fluorescence lifetimes (cellular autofluorescence: 1 to 2 ns; gold NPs: <0.02 ns; A488: 3.5 ns; H258: 2.5 ns). The NP-ACT-1 and A488-ACT-1 conjugates were bound homogeneously on the surface of cells, whereas H258 stained the cell nucleus. We demonstrate that the emission intensity of gold NPs is about ten times stronger than that of the autofluorescence of Karpas-299 cells at the same excitation power. Compared with fluorescent dyes, stronger emission is also observed from gold NPs. Together with their high photostability, these observations suggest that gold NPs are a viable alternative to fluorescent dyes for cellular imaging and cancer diagnosis.},
       ISBN = {1083-3668 (Print)
    1083-3668 (Linking)},
       year = {2008}
    }
  • Andrews, Peter M and Chen, Yu and Onozato, Maristela L and Huang, Shu-Wei and Adler, Desmond C and Huber, Robert A and Jiang, James and Barry, Scott E and Cable, Alex E and Fujimoto, James G: High-resolution optical coherence tomography imaging of the living kidney. Laboratory investigation; a journal of technical methods and pathology, no. 88, pp. 441-9, apr,, 2008
    BibTeX Link
    @Article{HU_2008_Andrews_a,
      Title                    = {{High-resolution optical coherence tomography imaging of the living kidney}},
      Author                   = {Andrews, Peter M and Chen, Yu and Onozato, Maristela L and Huang, Shu-Wei and Adler, Desmond C and Huber, Robert A and Jiang, James and Barry, Scott E and Cable, Alex E and Fujimoto, James G},
      Journal                  = {Laboratory investigation; a journal of technical methods and pathology},
      Year                     = {2008},
    
      Month                    = apr,
      Number                   = {4},
      Pages                    = {441--9},
      Volume                   = {88},
    
      Doi                      = {10.1038/labinvest.2008.4},
      ISSN                     = {1530-0307},
      Url                      = {http://www.nature.com/labinvest/journal/v88/n4/full/labinvest20084a.html}
    }
  • Framme, C and Roider, J and Brinkmann, R and Birngruber, R and Gabel, V-P: Grundlagen und klinische Anwendung der Lasertherapie an der Netzhaut. no. 225, pp. 259-268, 2008
    BibTeX
    @misc{Framme,
       author = {Framme, C and Roider, J and Brinkmann, R and Birngruber, R and Gabel, V-P},
       title = {Grundlagen und klinische Anwendung der Lasertherapie an der Netzhaut},
       volume = {225},
       number = {4},
       pages = {259-268},
       year = {2008}
    }
  • Jirauschek, Christian and Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert: Fourier domain mode locking theory. in 2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS, 2008
    BibTeX Link
    @InProceedings{HU_2008_Jirauschek_a,
      Title                    = {{Fourier domain mode locking theory}},
      Author                   = {Jirauschek, Christian and Eigenwillig, Christoph and Biedermann, Benjamin and Huber, Robert},
      Booktitle                = {2008 Conference on Quantum Electronics and Laser Science Conference on Lasers and Electro-Optics, CLEO/QELS},
      Year                     = {2008},
    
      Url                      = {http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=4571888&searchWithin%3Deigenwillig%26sortType%3Dasc_p_Sequence%26filter%3DAND%28p_IS_Number%3A4571172%29}
    }
  • Vogel, A. and Linz, N. and Freidank, S. and Paltauf, G.: Femtosecond-laser-induced nanocavitation in water: implications for optical breakdown threshold and cell surgery. no. 100, pp. 23, 2008
    BibTeX
    @misc{Vogel2008,
       author = {Vogel, A. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Femtosecond-laser-induced nanocavitation in water: implications for optical breakdown threshold and cell surgery},
       volume = {100},
       number = {3},
       pages = {23},
       note = {Using Smart Source Parsing
    Jan 25;:038102. Epub 2008 Jan},
       abstract = {We determined the bubble radius R_(max) for femtosecond optical breakdown in water at 347, 520, and 1040 nm with an unprecedented accuracy (+/-10 nm). At threshold, R_(max) was smaller than the diffraction-limited focus radius and ranged from 190 nm to 320 nm. The increase of R_(max) with laser energy E_(L) is slowest at 347 nm, providing optimum control of cell surgery. Experimental results agree with a model of bubble formation in heated and thermoelastically stretched liquids. Theory predicts a threshold temperature T_(th) approximately equal to 168 degrees C. For T>300 degrees C, a phase explosion sets in, and R_(max) increases rapidly with E_(L).},
       ISBN = {0031-9007 (Print)
    0031-9007 (Linking)},
       year = {2008}
    }
  • Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.: Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue. Invest Ophthalmol Vis Sci, no. 49, pp. 1512-7, 2008
    BibTeX
    @article{Steven,
       author = {Steven, P. and Rupp, J. and Huttmann, G. and Koop, N. and Lensing, C. and Laqua, H. and Gebert, A.},
       title = {Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {49},
       number = {4},
       pages = {1512-7},
       note = {Using Smart Source Parsing
    Apr},
       abstract = {PURPOSE: Conjunctiva-associated lymphoid tissue (CALT) is assumed to be a key location for the generation of adaptive immune mechanisms of the ocular surface, but functional studies of CALT are still lacking. The purpose of this study was to establish an animal model that enables functional analysis of immune mechanisms going on within CALT. In addition, the use of two-photon microscopy, a new optical method, was evaluated for examining complex immunologic interactions of CALT by volume (three-dimensional [3-D]) and time-dependence (four-dimensional [4-D]) in vivo. METHODS: The conjunctiva of female BALB/c mice was repeatedly challenged with topical Chlamydia trachomatis serovar C or a solution of ovalbumin and cholera toxin B. Two-photon microscopy was conducted on explanted, unfixed, and unstained eyes with adjacent nictitating membranes. RESULTS: After three to five stimulations, CALT was detected exclusively in the nictitating membrane of 73% (C. trachomatis) or 70% (ovalbumin/ cholera toxin) of the animals. CALT mainly consisted of CD45R/B220+ B cells and CD4+ and CD8+ T cells. Electron microscopy showed intraepithelial lymphocytes and follicles consisting of lymphocytes, dendritic cells, and macrophages. Two-photon microscopy based on tissue autofluorescence allowed all components of CALT to be detected three dimensionally. High-resolution images were generated in tissue depths of 65 microm below the mucosal surface. CONCLUSIONS: This study introduces a novel mouse model for functional investigations of CALT. Topical stimulation with C. trachomatis or ovalbumin/cholera toxin B reliably leads to CALT generation at the nictitating membrane. The use of two-photon microscopy enables groundbreaking 3-D and, in the future, intravital 4-D investigations of immunologic processes initiated in CALT.},
       year = {2008}
    }
  • Framme, C. and Walter, A. and Prahs, P. and Theisen-Kunde, D. and Brinkmann, R.: Comparison of threshold irradiances and online dosimetry for selective retina treatment (SRT) in patients treated with 200 nanoseconds and 1.7 microseconds laser pulses. Lasers Surg Med, no. 40, pp. 616-24, 2008
    BibTeX
    @article{Framme,
       author = {Framme, C. and Walter, A. and Prahs, P. and Theisen-Kunde, D. and Brinkmann, R.},
       title = {Comparison of threshold irradiances and online dosimetry for selective retina treatment (SRT) in patients treated with 200 nanoseconds and 1.7 microseconds laser pulses},
       journal = {Lasers Surg Med},
       volume = {40},
       number = {9},
       pages = {616-24},
       note = {Framme, Carsten
    Walter, Andreas
    Prahs, Philipp
    Theisen-Kunde, Dirk
    Brinkmann, Ralf
    Clinical Trial
    Comparative Study
    Research Support, Non-U.S. Gov't
    United States
    Lasers Surg Med. 2008 Nov;40(9):616-24.},
       abstract = {BACKGROUND: Selective retina therapy (SRT) solely affecting the RPE while sparing of the photoreceptors is usually performed with a train of repetitive laser pulses of 1.7 microseconds in duration. It was our purpose to evaluate the principle feasibility of SRT with shorter 200 nanoseconds laser pulses in patients. METHODS: Nineteen patients with macular disorders [diabetic maculopathy (DMP), geographic atrophy (GA), drusen maculopathy and central serous chorioretinopathy (CSR)] were treated with a prototype of a SRT laser (Nd:YLF laser; 527 nm; 1.7 microseconds and 200 nanoseconds pulse duration; 30 pulses at 100 Hz; spot size: 200 microm). Test lesions (n = 175) with increasing energy were applied at the lower arcade to determine the individual angiographic and ophthalmoscopic threshold radiant exposures (therapeutic window) before applying the central treatment lesions within these ranges additionally guided by online optoacoustic measurements. Postoperatively RPE damage was visualized and confirmed by fluorescein angiographic leakage and correlated with optoacoustic results. Additionally ED(50) damage thresholds were calculated by probit analysis. RESULTS: None of the short repetitive 200 nanoseconds laser pulses led to retinal hemorrhages or retinal ruptures. Nearly all of the test- and treatment lesions could be visualized by angiography indicating desired RPE damage but were ophthalmoscopically invisible suggesting intact neurosensory retinal structures. ED(50) cell damage threshold energies were significantly lower using 200 nanoseconds (99.6 microJ; n = 122) instead of 1.7 microseconds (196.3 microJ; n = 53) laser pulses. Optoacoustic and angiographic visibility correlated in 83.7% (200 nanoseconds) and 87.5% (1.7 microseconds). CONCLUSIONS: Selective RPE effects can safely be achieved using shorter 200 nanoseconds laser pulses in patients without adverse effects to the neurosensory retina. The required pulse energy compared to the standard 1.7 microseconds regime was reduced by about a factor of 2 suggesting a reduced heat generation and flow into adjacent tissues during the shorter laser impact and thus possibly enhancing selectivity. Optoacoustics also seem to be a viable alternative in 200 nanoseconds treatment for a non-invasive online dosimetry control system.},
       keywords = {Adult
    Aged
    Aged, 80 and over
    Cohort Studies
    Feasibility Studies
    Female
    Humans
    Laser Coagulation/ methods
    Lasers, Semiconductor/ therapeutic use
    Male
    Middle Aged
    Ophthalmoscopy
    Radiometry
    Retinal Diseases/pathology/radiography/ surgery
    Retinal Pigment Epithelium/pathology/radiation effects/radiography},
       year = {2008}
    }
    
  • Framme, C. and Sachs, H. G. and Kobuch, K. and Flucke, B. and Birngruber, R.: Clinical evaluation of experimentally induced choroidal neovascularizations in Pigmented rabbits by subretinal injection of lipid hydroperoxide and consecutive preliminary photodynamic treatment with tookad. Ophthalmologica, no. 222, pp. 254-264, 2008
    BibTeX Link
    @article{Framme2008,
       author = {Framme, C. and Sachs, H. G. and Kobuch, K. and Flucke, B. and Birngruber, R.},
       title = {Clinical evaluation of experimentally induced choroidal neovascularizations in Pigmented rabbits by subretinal injection of lipid hydroperoxide and consecutive preliminary photodynamic treatment with tookad},
       journal = {Ophthalmologica},
       volume = {222},
       number = {4},
       pages = {254-264},
       note = {338UZ
    Times Cited:3
    Cited References Count:27},
       abstract = {Purpose: Up to date several approaches have been undertaken to achieve an 'easy-to-handle' animal model of choroidal neovascularizations (CNVs) in rabbits; however, so far in none of the studies could healthy retinal tissue be maintained, which is mandatory to further investigate the effects of photodynamic therapy (PDT) or anti-vascular-endothelial-growth-factor treatments. It was our aim to reevaluate and verify the method of inducing experimental CNVs in rabbits using subretinally injected linoleic acid hydroperoxicle (LHP) as proposed by Tamai et al. and to use it for experimental PDT. Material and Methods: In 33 eyes of Chinchilla breed rabbits LHP of two different concentrations (25 and 100 mu g/50 mu l) was injected into the subretinal space via a transvitreal approach under guidance of an operation microscope. Ophthalmoscopic and angiographic examinations were performed on days 3, 7, 14 and 28 after surgery. Preliminary PDT with different experimental parameter sets was performed in 3 eyes using the new photosensitizer Tookad. Results: Using LHP in the higher concentration, an angiographically determined CNV induction was observed in 27% of all injection sites (n = 34) on days 14 and 28 revealing early well-demarcated and progressive leakage. No CNV was detected at the lower LHP concentration (60 injection sites). Subretinal CNV was verified histologically revealing vessel formation above the retinal pigment epithelium level. Herein, a significant damage to the outer retinal layers was always observed; however, the general structure of the choriocapillary layer was maintained. Tookad PDT was clinically able to completely stop leakage in I case and reduce leakage in 2 cases. Histologically the choriocapillary layer was occluded. Conclusion: Subretinal injection of LHP induces angiographically well-demarcated classic CNVs in rabbits; however, the CNV rate was low, and histology revealed severe damage of the outer retinal layers but not of the choriocapillary layer, which is important for studying PDT interactions. Preliminary experimental PDT could clinically stop or reduce leakage from angiographic CNV. Due to the small CNV rate and the significant collateral retinal tissue damage, this model seems to be only of partial suitability for investigating new treatment modalities in CNV. Copyright (c) 2008 S. Karger AG, Basel.},
       keywords = {age-related macular degeneration
    animal model
    choroidal neovascularization
    retinal pigment epithelium
    linoleic acid hydroperoxide
    senile macular degeneration
    bruchs membrane
    ranibizumab
    verteporfin
    therapy
    growth
    model},
       ISSN = {0030-3755},
       DOI = {Doi 10.1159/000136903},
       url = {<Go to ISI>://WOS:000258536200008},
       year = {2008},
       type = {Journal Article}
    }
    
  • Framme, C. and Roider, J. and Brinkmann, R. and Birngruber, R. and Gabel, V. P.: Basic principles and clinical application of retinal laser therapy. Klinische Monatsblatter Fur Augenheilkunde, no. 225, pp. 259-268, 2008
    BibTeX Link
    @article{Framme2008,
       author = {Framme, C. and Roider, J. and Brinkmann, R. and Birngruber, R. and Gabel, V. P.},
       title = {Basic principles and clinical application of retinal laser therapy},
       journal = {Klinische Monatsblatter Fur Augenheilkunde},
       volume = {225},
       number = {4},
       pages = {259-268},
       note = {301AZ
    Times Cited:3
    Cited References Count:39},
       abstract = {The scientific background of laser photocoagulation of the ocular fundus was studied extensively by several investigators in the 1970 s and 1980 s. The basic principles were succesfully resolved during that time and clinical consequences for proper application of the laser photocoagulation for various diseases were deduced. The present paper gives an overview about the physical basics of laser-tissue interactions during and after retinal laser treatment and the particular laser strategies in the treatment of different retinal diseases. Thus, it addresses the issue of the impact on tissue of laser parameters as wavelength, spot size, pulse duration and laser power. Additionally, the different biological tissue reactions after laser treatment are presented, such as, e.g., for retinopexia or macular treatments as well as for diabetic retinopathies. Specific laser strategies such as the selective laser treatment of the RPE (SRT) or the transpupillary thermotherapy (TTT) are presented and discussed.},
       keywords = {retina
    anatomy
    vitreous
    subfoveal choroidal neovascularization
    central vein occlusion
    transpupillary thermotherapy
    macular degeneration
    pigment epithelium
    photocoagulation
    argon
    trial
    rpe
    diseases},
       ISSN = {0023-2165},
       DOI = {DOI 10.1055/s-2008-1027202},
       url = {<Go to ISI>://WOS:000255870100001},
       year = {2008},
       type = {Journal Article}
    }
    

2007

  • Kranendonk, Laura A and Huber, Robert and Fujimoto, James G and Sanders, Scott T: Wavelength-agile H2O absorption spectrometer for thermometry of general combustion gases. Proceedings of the Combustion Institute, no. 31, pp. 783-790, jan,, 2007
    BibTeX Link
    @Article{HU_2007_Kranendonk_b,
      Title                    = {{Wavelength-agile H2O absorption spectrometer for thermometry of general combustion gases}},
      Author                   = {Kranendonk, Laura A and Huber, Robert and Fujimoto, James G and Sanders, Scott T},
      Journal                  = {Proceedings of the Combustion Institute},
      Year                     = {2007},
    
      Month                    = jan,
      Number                   = {1},
      Pages                    = {783--790},
      Volume                   = {31},
    
      Doi                      = {10.1016/j.proci.2006.08.003},
      ISSN                     = {15407489},
      Url                      = {http://linkinghub.elsevier.com/retrieve/pii/S1540748906002665}
    }
  • Jenkins, M W and Adler, D C and Gargesha, M and Huber, R and Rothenberg, F and Belding, J and Watanabe, M and Wilson, D L and Fujimoto, J G and Rollins, A M: Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser. Optics Express, no. 15, pp. 6251 - 6267, 2007
    BibTeX Link
    @Article{HU_2007_Jenkins_a,
      Title                    = {{Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser}},
      Author                   = {Jenkins, M W and Adler, D C and Gargesha, M and Huber, R and Rothenberg, F and Belding, J and Watanabe, M and Wilson, D L and Fujimoto, J G and Rollins, A M},
      Journal                  = {Optics Express},
      Year                     = {2007},
      Number                   = {10},
      Pages                    = {6251 -- 6267 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.006251},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-10-6251}
    }
  • Steven, P. and Rupp, J. and Huettmann, G. and Koop, N. and Laqua, H.: Two-Photon Real-Time Imaging of Conjunctiva-Associated Lymphoid Tissue (CALT). Investigative Ophthalmology & Visual Science, no. 48, pp. 201-201, 2007
    BibTeX Link
    @article{Steven2007,
       author = {Steven, P. and Rupp, J. and Huettmann, G. and Koop, N. and Laqua, H.},
       title = {Two-Photon Real-Time Imaging of Conjunctiva-Associated Lymphoid Tissue (CALT)},
       journal = {Investigative Ophthalmology & Visual Science},
       volume = {48},
       number = {13},
       pages = {201-201},
       abstract = {AbstractPurpose:: Immunological real-time analysis of conjunctiva-associated lymphoid tissue (CALT) is challenging at state. For the first time, two-photon microscopy, a new optical method, is evaluated in its use to analyze morphology and function of CALT. Methods:: Conjunctiva of female Balb/c mice is challenged with Chlamydia trachomatis serovar C (ChtC) or ovalbumin and choleratoxin subunit B (OVA/CTB) for CALT induction. A two-photon microscope equipped with a near infrared femtosecond-laser and a fluorescence-lifetime detector is used for ex-vivo analysis of unfixed and unstained ocular tissue with additional application of fluorescent microspheres to demonstrate transepithelial particle transport. Results:: Challenge with ChtC or OVA/CTB induce all CALT components (lymphoepithelium, follicles, blood and lymphatic vessels), that are demonstrated in cellular and subcellular resolution by means of autofluorescence imaging. Wavelength adaptation allows specific differentiation of cellular and acellular components. Fluorescence-lifetime detection permits differentiation of cellular subsets (e.g. lymphocytes and macrophages). Application of fluorescent microspheres demonstrates transepithelial particle transport and detection within intracellular vesicles. Conclusions:: Two-photonmicroscopy is an innovative optical technique to analyse morphological and functional features of CALT. Detection of transepithelial particle transport and its impact on conjunctival immunological processes can be visualized in real-time. Future in-vivo experiments with suitable animal models would allow detailed analysis of CALT in a clinical context e.g. corneal transplant rejection, keratoconjunctivitis sicca and follicular conjunctivitis.},
       ISSN = {1552-5783},
       url = {http://dx.doi.org/},
       year = {2007},
       type = {Journal Article}
    }
    
  • Ramrath, Lukas and Hofmann, UlrichG and Huettmann, Gereon and Moser, Andreas and Schweikard, Achim: Towards Automated OCT-based Identification of White Brain Matter. in Bildverarbeitung für die Medizin 2007, pp. 414-418, Springer Berlin Heidelberg, 2007
    BibTeX
    @inbook{Ramrath,
       author = {Ramrath, Lukas and Hofmann, UlrichG and Huettmann, Gereon and Moser, Andreas and Schweikard, Achim},
       title = {Towards Automated OCT-based Identification of White Brain Matter},
       booktitle = {Bildverarbeitung für die Medizin 2007},
       editor = {Horsch, Alexander and Deserno, ThomasM and Handels, Heinz and Meinzer, Hans-Peter and Tolxdorff, Thomas},
       series = {Informatik aktuell},
       publisher = {Springer Berlin Heidelberg},
       chapter = {83},
       pages = {414-418},
       year = {2007}
    }
  • Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G: Three-dimensional endomicroscopy using optical coherence tomography. Nature Photonics, no. 1, pp. 709-716, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Adler_a,
      Title                    = {{Three-dimensional endomicroscopy using optical coherence tomography}},
      Author                   = {Adler, Desmond C and Chen, Yu and Huber, Robert and Schmitt, Joseph and Connolly, James and Fujimoto, James G},
      Journal                  = {Nature Photonics},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {12},
      Pages                    = {709--716},
      Volume                   = {1},
    
      Doi                      = {10.1038/nphoton.2007.228},
      ISSN                     = {1749-4885},
      Url                      = {http://www.nature.com/doifinder/10.1038/nphoton.2007.228}
    }
  • Huang, Shu-Wei and Aguirre, Aaron D and Huber, Robert A and Adler, Desmond C and Fujimoto, James G: Swept source optical coherence microscopy using a Fourier domain mode-locked laser. Optics Express, no. 15, pp. 6210 - 6217, 2007
    BibTeX Link
    @Article{HU_2007_Huang_a,
      Title                    = {{Swept source optical coherence microscopy using a Fourier domain mode-locked laser}},
      Author                   = {Huang, Shu-Wei and Aguirre, Aaron D and Huber, Robert A and Adler, Desmond C and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2007},
      Number                   = {10},
      Pages                    = {6210 -- 6217 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.006210},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-10-6210}
    }
  • Brinkmann, R. and Birngruber, R.: Selektive Retina-Therapie (SRT). Zeitschrift für Medizinische Physik, no. 17, pp. 6-22, 2007
    BibTeX Link
    @article{Brinkmann2007,
       author = {Brinkmann, R. and Birngruber, R.},
       title = {Selektive Retina-Therapie (SRT)},
       journal = {Zeitschrift für Medizinische Physik},
       volume = {17},
       number = {1},
       pages = {6-22},
       note = {276HP
    Times Cited:0
    Cited References Count:42},
       abstract = {Selective Retina Therapy (SRT) is a new and very gentle laser method developed at the Medical Laser Center Lubeck. It is currently investigated clinically in order to treat retinal disorders associated with a decreased function of the retinal pigment epithelium (RPE). SRT is designed to selectively effect the RPE while sparing the neural retina and the photoreceptors as well as the chorioidea. Aim of the therapy is the rejuvenation of the RPE in the treated areas, which should ideally lead to a long term metabolic increase at the chorio- retinal junction. In contrast to conventional laser photocoagulation, which is associated with a complete thermal necrosis of the treated site, SRT completely retains full vision. This paper reviews the methods and mechanisms behind selective RPE effects and reports the first clinical results. An online dosimetry technique to visualize the ophthalmoscopically invisible effects is introduced.},
       keywords = {selective cellular effects
    optoacoustics
    online dosimetry
    rpe
    mu s-laser pulses
    macula oedema
    rcs
    pigment epithelium
    diabetic maculopathy
    laser irradiation
    time regimen
    damage
    rpe
    photocoagulation
    absorption
    mechanisms
    radiation},
       ISSN = {0939-3889},
       DOI = {DOI 10.1016/j.zemedi.2006.11.002},
       url = {<Go to ISI>://WOS:000254132200002},
       year = {2007},
       type = {Journal Article}
    }
    
  • Framme, C. and Alt, C. and Schnell, S. and Sherwood, M. and Brinkmann, R. and Lin, C. P.: Selective targeting of the retinal pigment epithelium in rabbit eyes with a scanning laser beam. Invest Ophthalmol Vis Sci, no. 48, pp. 1782-92, 2007
    BibTeX
    @article{Framme,
       author = {Framme, C. and Alt, C. and Schnell, S. and Sherwood, M. and Brinkmann, R. and Lin, C. P.},
       title = {Selective targeting of the retinal pigment epithelium in rabbit eyes with a scanning laser beam},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {48},
       number = {4},
       pages = {1782-92},
       note = {0146-0404 (Print)
    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't},
       abstract = {PURPOSE: Selective targeting of the retinal pigment epithelium (RPE) with repetitive laser pulses that minimize thermal damage to the adjacent photoreceptors is a promising new therapeutic modality for RPE-related retinal diseases. The selectivity of an alternative, more versatile scanning approach was examined in vivo by using a broad range of scanning parameters. METHODS: Acousto-optic deflectors repeatedly scanned the focus of a continuous wave (cw)-laser across the retina of Dutch belted rabbits, producing microsecond irradiation at each RPE cell. Two irradiation patterns forming separated lines (SEP) or interlaced lines (INT), different dwell times (2.5-75 micros), and repetition numbers (10 and 100 scans with 100-Hz repetition rate) were tested. Thresholds were evaluated by fundus imaging and angiography. Histology was performed for selected parameters. RESULTS: Selective RPE cell damage was obtained with moderate laser power. The angiographic threshold power decreased with pulse duration, number of exposures, and applying the INT pattern. Ophthalmoscopic thresholds, indicating onset of thermal coagulation, were higher than twice the angiographic threshold for most tested parameters. Histology confirmed selective RPE cell damage for SEP irradiation with 7.5 and 15 micros; slower scan speeds or closed lines caused photoreceptor damage. CONCLUSIONS: A cw-laser scanner can be set up as a highly compact and versatile device. Selective RPE damage is feasible with dwell times up to 15 micros. Greatest selectivity is achieved with short exposure times and separated scan lines. Interlaced lines and long exposure times facilitate heat conduction into photoreceptors. A scanner is an attractive alternative for pulsed selective targeting, because both selective targeting and thermal photocoagulation can be realized.},
       keywords = {Animals
    Eye Injuries/diagnosis
    Fluorescein Angiography
    Laser Coagulation/adverse effects/instrumentation/*methods
    Ophthalmoscopy
    Photoreceptors, Vertebrate/pathology
    Pigment Epithelium of Eye/injuries/pathology/*surgery
    Rabbits
    Retina/injuries/pathology},
       year = {2007}
    }
  • Brinkmann, R and Stalljohann, J and Weber, B and Schlott, K and Kandulla, J and Birngruber, R: Retinal Temperature Determination During Laser Photocoagulation. Proc SPIE, no. 6632, pp. 8, 2007
    BibTeX
    @article{Brinkmann2007,
       author = {Brinkmann, R and Stalljohann, J and Weber, B  and Schlott, K and Kandulla, J and Birngruber, R},
       title = {Retinal Temperature Determination During Laser Photocoagulation},
       journal = {Proc SPIE},
       volume = {6632},
       pages = {8},
       year = {2007},
       type = {Journal Article}
    }
    
  • Horneffer, V. and Linz, N. and Vogel, A.: Principles of laser-induced separation and transport of living cells. J Biomed Opt, no. 12, pp. 054016, 2007
    BibTeX
    @article{Horneffer,
       author = {Horneffer, V. and Linz, N. and Vogel, A.},
       title = {Principles of laser-induced separation and transport of living cells},
       journal = {J Biomed Opt},
       volume = {12},
       number = {5},
       pages = {054016},
       note = {Horneffer, Verena
    Linz, Norbert
    Vogel, Alfred
    Evaluation Studies
    Research Support, Non-U.S. Gov't
    United States
    J Biomed Opt. 2007 Sep-Oct;12(5):054016.},
       abstract = {Separation and transport of defined populations of living cells grown on a thin membrane can be achieved by laser microdissection (LMD) of the sample of interest, followed by a laser-induced forward transport process [laser pressure "catapulting" (LPC)] of the dissected cell cluster. We investigate the dynamics of LMD and LPC with focused and defocused UV-A laser pulses by means of time-resolved photography. Catapulting is driven by plasma formation when tightly focused pulses are used, and by confined thermal ablation at the bottom of the sample for defocused catapulting. With both modalities, the initial specimen velocity amounts to about 50 to 60 ms. Time-resolved photography of live cell catapulting reveals that in defocused catapulting, strong shear forces arise when the sample is accelerated out of the culture medium covering the cells. By contrast, pulses focused at the periphery of the specimen cause a fast rotational movement that minimizes the flow of culture medium parallel to the sample surface, and thus the resulting shear stresses. Therefore, the recultivation rate of catapulted cells is much higher when focused pulses are used. Compared to collateral damage by mechanical forces, side effects by heat and UV exposure of the cells play only a minor role.},
       keywords = {Animals
    CHO Cells
    Cell Separation/ methods
    Cricetinae
    Cricetulus
    Microdissection/ methods
    Optical Tweezers
    Specimen Handling/ methods},
       year = {2007}
    }
  • Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.: Principles of laser microdissection and catapulting of histologic specimens and live cells. in Laser Manipulation of Cells and Tissues, Methods in Cell Biology, no. 82, pp. 153-205, Academic Press Elsevier, San Diego, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Horneffer, V. and Lorenz, B. and Linz, N. and Freidank, S. and Hüttmann, G. and Gebert, A.},
       title = {Principles of laser microdissection and catapulting of histologic specimens and live cells},
       booktitle = {Laser Manipulation of Cells and Tissues, Methods in Cell Biology},
       editor = {Berns, M.  and Greulich, K.O.},
       publisher = {Academic Press Elsevier},
       address = {San Diego},
       volume = {82},
       pages = {153-205},
       year = {2007}
    }
  • Theisen-Kunde, D and Ott, V and Brinkmann, R and Keller, R: Potential of a new cw 2µm laser scalpel for laparoscopic surgery. Med. Laser Appl., no. 22, pp. 139-45, 2007
    BibTeX
    @article{Theisen-Kunde,
       author = {Theisen-Kunde, D and Ott, V and Brinkmann, R and Keller, R},
       title = {Potential of a new cw 2µm laser scalpel for laparoscopic surgery},
       journal = {Med. Laser Appl.},
       volume = {22},
       number = {2},
       pages = {139-45},
       abstract = {Abstract
    The potential of a new continuous-wave (cw) thulium–YAG laser for laparoscopic small intestine resection was investigated in pigs in comparison to standard bipolar scissors.
    
    Method
    A diode-pumped solid-state thulium–YAG laser system emitting at a wavelength of 2.01 µm was used. Laser power up to 25 W in cw mode was transmitted via a quartz fibre (400 µm core diameter). In order to resect 1 m of the small intestine, the accompanied mesentery was dissected with both devices in 12 pigs (six each group). Arteries and veins of 0.25–3.2 mm inner diameter were dissected in vivo and the resistance of the occluded vessels to pressures up to 375 mmHg was measured by an in vitro set-up. Samples were prepared for histological evaluation.
    
    Results
    With respect to intestine resection with bipolar scissors, bleeding occurred significantly less (25%) and dissection time was reduced by 19% using the 2 µm laser scalpel. With the 2 µm laser scalpel, small vessels (<0.5 mm) were successfully occluded up to 100% (arteries) and 89% (veins), larger vessels (1–2.3 mm) to 74% (arteries) and 65% (veins) in vivo. In the in vitro pressure measurement with 375 mmHg, 30% of veins and 35% of arteries stayed closed. In conclusion, the first experiments show that the 2 µm laser scalpel is a promising dissection device for minimally invasive surgery. 
    Zusammenfassung
    In einer vergleichenden Tierstudie wurde das Potential eines neuen cw Thulium–YAG-Lasersystems mit dem einer kommerziell erhältlichen bipolaren Schere zur laparoskopischen Resektion des Dünndarms verglichen.
    
    Methode
    Es wurde ein diodengepumptes Thulium–YAG-Festkörper-Lasersystem mit einer Emissionswellenlänge von 2,01 µm verwendet. Die Laserleistung betrug 25 W (Dauerstrich) und die Laserstrahlung wurde mittels einer Quarzglasfaser (Kerndurchmesser 400 µm) zum Applikationsort transmittiert. Zur Entnahme von 1 m Dünndarm wurde zunächst mit beiden Dissektionsgeräten das angrenzende Mesenterium an 12 Schweinen (6 je Instrument) durchtrennt. Zur Untersuchung der Hämostaseeigenschaften des 2 µm Laserskalpells wurden ausgewählte Arterien und Venen mit einem inneren Durchmesser von 0,25 bis 3,2 mm in vivo durchtrennt. In einer anschließenden in vitro Untersuchung wurde der Gefäßverschluss mit einem Druck von 375 mmHg überprüft. Zur histologischen Auswertung wurden Gewebeproben entnommen, mittels H&E gefärbt und lichtmikroskopisch untersucht.
    
    Ergebnisse
    Im Vergleich zur konventionellen bipolaren Schere konnte in der durchgeführten Studie die Anzahl der auftretenden Blutungen mit dem 2 µm Laserskalpell signifikant (25%) und die gesamte Resektionszeit um 19% (nicht signifikant) reduziert werden. Die Hämostase von Gefäßen, welche mittels des 2 µm Laserskalpells durchtrennt wurden, betrug bei Durchmessern <0.5 mm 100% bei den Arterien und 89% bei den Venen. Bei größeren Gefäßen (1–2.3 mm innerer Durchmesser) wurden 74% der Arterien und 65% der Venen dauerhaft in vivo koaguliert. Bei den in vitro Untersuchungen der koagulierten Gefäße mit einem Druck von 375 mmHg waren noch insgesamt 35% der Arterien und 30% der Venen verschlossen.
    
    In dieser Studie konnte gezeigt werden, dass das 2 µm Laserskalpell ein vielversprechendes Instrument für die laparoskopische Chirurgie darstellt.
    
    Resúmen
    En este estudio se investigó el potencial del nuevo láser cw thulium–YAG comparado al uso de la tijera bipolar estándar en intervenciones laparoscópicas en intestino delgado de cerdos.
    
    Método
    Se utilizó un láser thulium–YAG de estado sólido bombeado por diodos que emite en una longitud de onda de 2.01 µm. Una potencia láser de hasta 25 W de onda continua (cw) fue trasmitida a través de una fibra de cuarzo de 400 µm de diámetro. El mesenterio que acompaña al intestino delgado fue diseccionado utilizando uno de los dos sistemas en 12 cerdos (6 con cada instrumento). Se seccionaron in vivo, arterias y venas de 0.25 a 3.2 mm de diámetro interno y se midió in vitro la resistencia de los vasos ocluídos con una presión de hasta 375 mmHg. Las muestras fueron preparadas luego para evaluaciones histológicas.
    
    Resultados
    En la resección de intestino, el uso del bisturí láser de 2 µm ofrece menor sangrado (25%) y el reduce el tiempo de dissección en un 19% con respecto a las tijeras bipolares. Además, el uso del láser permite la oclusión in vivo de los pequeños vasos (diámetro menor a 0,5 mm) hasta en un 100% en arterias y un 89% en venas. Mientras que para las de mayor diámetro (de 1 a 2.3 mm) se obtuvo un 74% y 65%, respectivamente. En las mediciones in vitro de presión con 375 mmHg, un 30% de las venas y un 35% de las arterias permanecieron cerradas. En conclusión, estos primeros experimentos demuestran que el bisturí láser de 2 µm es una herramienta de disección prometedora para la cirugía laparoscópica. 
    
    Keywords: Thulium laser; Minimally invasive; Vessel sealing; Laser scalpel
    Schlüsselwörter: Thuliumlaser; Minimalinvasiv; Hämostase; Laserskalpell
    Palabras clave: Thulium láser; Cirugía mínimamente invasiva; Oclusión de vasos; Bisturí láser},
       year = {2007}
    }
  • Hüttmann, G. and Löning, M.: Physikalische Grundlagen optischer Technologien. Der Gynäkologe, no. 40, pp. 332-337, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G. and Löning, M.},
       title = {Physikalische Grundlagen optischer Technologien},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {332-337},
       year = {2007}
    }
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Phase-sensitive optical coherence tomography using buffered Fourier domain mode-locked lasers at up to 370,000 scans per second. in Biomedical Optics (BiOS) 2007, pp. 64291L-64291L-9, International Society for Optics and Photonics, feb,, 2007
    BibTeX Link
    @InProceedings{HU_2007_Adler_d,
      Title                    = {{Phase-sensitive optical coherence tomography using buffered Fourier domain mode-locked lasers at up to 370,000 scans per second}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Booktitle                = {Biomedical Optics (BiOS) 2007},
      Year                     = {2007},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {64291L--64291L--9},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.704128},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1334093}
    }
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers. Optics Letters, no. 32, pp. 626 - 628, 2007
    BibTeX Link
    @Article{HU_2007_Adler_c,
      Title                    = {{Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Letters},
      Year                     = {2007},
      Number                   = {6},
      Pages                    = {626 -- 628},
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.000626},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-6-626}
    }
    
  • Vogel, Alfred and Apitz, Ingo and Venugopalan, Vasan: Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues. in Oscillations, Waves and Interactions, T. Kurz, U. Parlitz, and U. Kaatze, eds.(Universitätsverlag Göttingen, Göttingen, 2007), pp. 217-258, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Apitz, Ingo and Venugopalan, Vasan},
       title = {Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues},
       booktitle = {Oscillations, Waves and Interactions, T. Kurz, U. Parlitz, and U. Kaatze, eds.(Universitätsverlag Göttingen, Göttingen, 2007)},
       pages = {217-258},
       year = {2007}
    }
    
  • Vogel, A and Apitz, I. and Venugopalan, V.: Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues. in Oscillations, Waves, and Interactions. Sixty Years Drittes Physikalisches Institut., no. A Festschrift, pp. 217-258, Universitätsverlag, Göttingen, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Apitz, I. and Venugopalan, V.},
       title = {Phase transitions, material ejection, and plume dynamics in pulsed laser ablation of soft biological tissues},
       booktitle = {Oscillations, Waves, and Interactions. Sixty Years Drittes Physikalisches Institut.},
       editor = {Kurtz, T., Parlitz, U. and  Kaatze, U.},
       publisher = {Universitätsverlag},
       address = {Göttingen},
       volume = {A Festschrift},
       pages = {217-258},
       year = {2007}
    }
  • Quaden, R. and Klaws, G. R. and Theisen-Kunde, D. and Lutter, G.: Percutaneous aortic valve replacement: first endovascular resection of human aortic valves in situ. Eur J Cardiothorac Surg, no. 31, pp. 305, 2007
    BibTeX
    @article{Quaden,
       author = {Quaden, R. and Klaws, G. R. and Theisen-Kunde, D. and Lutter, G.},
       title = {Percutaneous aortic valve replacement: first endovascular resection of human aortic valves in situ},
       journal = {Eur J Cardiothorac Surg},
       volume = {31},
       number = {2},
       pages = {305},
       note = {1010-7940 (Print)
    Journal Article},
       keywords = {Aortic Valve/*surgery
    Endoscopy
    Heart Valve Prosthesis Implantation/*methods
    Humans
    Laser Surgery/methods
    Surgical Procedures, Minimally Invasive/*methods},
       year = {2007}
    }
  • Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.: Optische Kohärenztomographie in der Gynokologie. Der Gynäkologe, no. 40, 2007
    BibTeX
    @article{Löning,
       author = {Löning, M. and Lankenau, E. and Diddens, H. and Krokowski, M. and Diedrich, K. and Hüttmann, G.},
       title = {Optische Kohärenztomographie in der Gynokologie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       year = {2007}
    }
  • Adler, Desmond C and Huber, Robert and Fujimoto, James G: Optical coherence tomography phase microscopy using buffered fourier domain mode locked (FDML) lasers at up to 370,000 lines per second. in Conference on Lasers and Electro-Optics, 2007, CLEO 2007, 2007
    BibTeX Link
    @InProceedings{HU_2007_Adler_e,
      Title                    = {{Optical coherence tomography phase microscopy using buffered fourier domain mode locked (FDML) lasers at up to 370,000 lines per second}},
      Author                   = {Adler, Desmond C and Huber, Robert and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics, 2007, CLEO 2007},
      Year                     = {2007},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=CLEO-2007-CFL1}
    }
  • Neumann, J and Brinkmann, R: Nucleation dynamics around single microabsorbers in water heated by nanosecond irradiation. J Appl Phys, no. 101, 2007
    BibTeX
    @article{Neumann,
       author = {Neumann, J and Brinkmann, R},
       title = {Nucleation dynamics around single microabsorbers in water heated by nanosecond irradiation},
       journal = {J Appl Phys},
       volume = {101},
       number = {114701},
       year = {2007}
    }
  • Kandulla, J. and Brinkmann, R: Nicht-invasive Echtzeit-Temperaturbestimmung während Laserbehandlungen an der Netzhaut des Auges. Photonik, no. 2, pp. 42-46, 2007
    BibTeX
    @article{Kandulla,
       author = {Kandulla, J. and Brinkmann, R},
       title = {Nicht-invasive Echtzeit-Temperaturbestimmung während Laserbehandlungen an der Netzhaut des Auges},
       journal = {Photonik},
       volume = {2},
       pages = {42-46},
       year = {2007}
    }
  • Rusanov, V. and Paulsen, H. and Böttger, L. H. and Winkler, H. and Wolny, J. A. and Koop, N. and Dorn, Th. and Janiak, C. and Trautwein, A. X.: Mössbauer, nuclear inelastic scattering and density functional studies on the second metastable state of Na2[Fe(CN)5NO]·2H2O. Hyperfine Interactions, no. 175, pp. 141-150, 2007
    BibTeX Link Link
    @article{Rusanov2007,
       author = {Rusanov, V. and Paulsen, H. and Böttger, L. H. and Winkler, H. and Wolny, J. A. and Koop, N. and Dorn, Th. and Janiak, C. and Trautwein, A. X.},
       title = {Mössbauer, nuclear inelastic scattering and density functional studies on the second metastable state of Na2[Fe(CN)5NO]·2H2O},
       journal = {Hyperfine Interactions},
       volume = {175},
       number = {1},
       pages = {141-150},
       abstract = {The structure of the light-induced metastable state SII of Na2[Fe(CN)5NO]·2H2O was investigated by transmission Mössbauer spectroscopy (TMS) in the temperature range between 85 and 135 K, nuclear inelastic scattering (NIS) at 98 K using synchrotron radiation and density functional theory (DFT) calculations. The DFT and TMS results strongly support the view that the NO group in SII takes a side-on molecular orientation and, further, is dynamically displaced from one eclipsed, via a staggered, to a second eclipsed orientation. The population conditions for generating SII are optimal for measurements by TMS, yet they are modest for accumulating NIS spectra. Optimization of population conditions for NIS measurements is discussed and new NIS experiments on SII are proposed.},
       ISSN = {1572-9540},
       DOI = {10.1007/s10751-008-9598-8},
       url = {http://dx.doi.org/10.1007/s10751-008-9598-8},
       year = {2007},
       type = {Journal Article}
    }
    
  • Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.: Mechanisms of laser-induced dissection and transport of histologic specimens.. Biophys J, no. 93, pp. 4481-4500, 2007
    BibTeX
    @article{Vogel,
       author = {Vogel, A. and Lorenz, K. and Horneffer, V. and Hüttmann, G. and von Smolinski, D. and Gebert, A.},
       title = {Mechanisms of laser-induced dissection and transport of histologic specimens.},
       journal = {Biophys J},
       volume = {93},
       pages = {4481-4500},
       year = {2007}
    }
  • Vogel, Alfred and Lorenz, Kathrin and Horneffer, Verena and Hüttmann, Gereon and Von Smolinski, Dorthe and Gebert, Andreas: Mechanisms of laser-induced dissection and transport of histologic specimens. Biophysical journal, no. 93, pp. 4481-4500, 2007
    BibTeX
    @article{Vogel2007,
       author = {Vogel, Alfred and Lorenz, Kathrin and Horneffer, Verena and Hüttmann, Gereon and Von Smolinski, Dorthe and Gebert, Andreas},
       title = {Mechanisms of laser-induced dissection and transport of histologic specimens},
       journal = {Biophysical journal},
       volume = {93},
       number = {12},
       pages = {4481-4500},
       ISSN = {0006-3495},
       year = {2007},
       type = {Journal Article}
    }
    
  • Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.: Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues. in The Eigth International Conference on Laser Ablation (COLA 2005), no. 59, pp. 249-254, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A and Noack, J. and Hüttmann, G. and Paltauf, G.},
       title = {Mechanisms of femtosecond laser nanoprocessing of biological cells and tissues},
       booktitle = {The Eigth International Conference on Laser Ablation (COLA 2005)},
       editor = {Herman, P. and Hess, W .},
       series = {Journal of Physics: Conference Series},
       volume = {59},
       pages = {249-254},
       year = {2007}
    }
  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.: Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo. Neuro Oncol, no. 9, pp. 103-12, 2007
    BibTeX
    @article{Kantelhardt,
       author = {Kantelhardt, S. R. and Leppert, J. and Krajewski, J. and Petkus, N. and Reusche, E. and Tronnier, V. M. and Huttmann, G. and Giese, A.},
       title = {Imaging of brain and brain tumor specimens by time-resolved multiphoton excitation microscopy ex vivo},
       journal = {Neuro Oncol},
       volume = {9},
       number = {2},
       pages = {103-12},
       note = {Kantelhardt, Sven R
    Leppert, Jan
    Krajewski, Jochen
    Petkus, Nadine
    Reusche, Erich
    Tronnier, Volker M
    Huttmann, Gereon
    Giese, Alf
    United States
    Neuro Oncol. 2007 Apr;9(2):103-12. Epub 2007 Feb 26.},
       abstract = {Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this technology to the structural and photochemical imaging of cultured glioma cells and experimental gliomas ex vivo. We demonstrated that high microanatomical definition of the tumor, invasion zone, and normal adjacent brain can be obtained down to single-cell resolution in unprocessed tissue blocks. In this study, we used multiphoton excitation and four-dimensional microscopy to generate fluorescence lifetime maps of the murine brain anatomy, experimental glioma tissue, and biopsy specimens of human glial tumors. In murine brain, cellular and noncellular elements of the normal anatomy were identified. Distinct excitation profiles and lifetimes of endogenous fluorophores were identified for specific brain regions. Intracranial grafts of human glioma cell lines in mouse brain were used to study the excitation profiles and fluorescence lifetimes of tumor cells and adjacent host brain. These studies demonstrated that normal brain and tumor could be distinguished on the basis of fluorescence intensity and fluorescence lifetime profiles. Human brain specimens and brain tumor biopsies were also analyzed by multiphoton microscopy, which demonstrated distinct excitation and lifetime profiles in glioma specimens and tumor-adjacent brain. This study demonstrates that multiphoton excitation of autofluorescence can distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence. Further technical developments in this technology may provide a means for in situ tissue analysis, which might be used to detect residual tumor at the resection edge.},
       keywords = {Animals
    Brain/anatomy & histology/ pathology
    Brain Neoplasms/ pathology
    Disease Models, Animal
    Glioma/pathology
    Mice
    Mice, Inbred Strains
    Microscopy, Fluorescence, Multiphoton/instrumentation/ methods
    Sensitivity and Specificity},
       year = {2007}
    }
  • Srinivasan, V J and Huber, R and Gorczynska, I and Fujimoto, J G and Jiang, J Y and Reisen, P and Cable, A E: High-speed, high-resolution optical coherence tomography retinal imaging with a frequency-swept laser at 850 nm. Optics Letters, no. 32, pp. 361 - 363, OSA, 2007
    BibTeX Link
    @Article{HU_2007_Srinivasan_a,
      Title                    = {{High-speed, high-resolution optical coherence tomography retinal imaging with a frequency-swept laser at 850 nm}},
      Author                   = {Srinivasan, V J and Huber, R and Gorczynska, I and Fujimoto, J G and Jiang, J Y and Reisen, P and Cable, A E},
      Journal                  = {Optics Letters},
      Year                     = {2007},
      Number                   = {4},
      Pages                    = {361 -- 363 },
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.000361},
      ISSN                     = {0146-9592},
      Publisher                = {OSA},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-4-361}
    }
  • Kranendonk, Laura A and An, Xinliang and Caswell, Andrew W and Herold, Randy E and Sanders, Scott T and Huber, Robert and Fujimoto, James G and Okura, Yasuhiro and Urata, Yasuhiro: High speed engine gas thermometry by Fourier-domain mode-locked laser absorption spectroscopy. Optics Express, no. 15, pp. 15115 - 15128, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Kranendonk_a,
      Title                    = {{High speed engine gas thermometry by Fourier-domain mode-locked laser absorption spectroscopy}},
      Author                   = {Kranendonk, Laura A and An, Xinliang and Caswell, Andrew W and Herold, Randy E and Sanders, Scott T and Huber, Robert and Fujimoto, James G and Okura, Yasuhiro and Urata, Yasuhiro},
      Journal                  = {Optics Express},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {23},
      Pages                    = {15115 -- 15128 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.015115},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-23-15115}
    }
  • Huber, Robert A and Adler, Desmond C and Srinivasan, Vivek J and Gorczynska, Iwona M and Fujimoto, James G: Fourier domain mode-locked (FDML) lasers at 1050 nm and 202,000 sweeps per second for OCT retinal imaging. in Proceedings of SPIE, no. 6429, pp. 642907-642907-7, feb,, 2007
    BibTeX Link
    @InProceedings{HU_2007_Huber_c,
      Title                    = {{Fourier domain mode-locked (FDML) lasers at 1050 nm and 202,000 sweeps per second for OCT retinal imaging}},
      Author                   = {Huber, Robert A and Adler, Desmond C and Srinivasan, Vivek J and Gorczynska, Iwona M and Fujimoto, James G},
      Booktitle                = {Proceedings of SPIE},
      Year                     = {2007},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {642907--642907--7},
      Volume                   = {6429},
    
      Doi                      = {10.1117/12.704084},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1296450}
    }
  • Huber, R and Adler, D C and Srinivasan, V J and Fujimoto, J G: Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second. Optics Letters, no. 32, pp. 2049 - 2051, jul,, 2007
    BibTeX Link
    @Article{HU_2007_Huber_a,
      Title                    = {{Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second}},
      Author                   = {Huber, R and Adler, D C and Srinivasan, V J and Fujimoto, J G},
      Journal                  = {Optics Letters},
      Year                     = {2007},
    
      Month                    = jul,
      Number                   = {14},
      Pages                    = {2049 -- 2051},
      Volume                   = {32},
    
      Doi                      = {10.1364/OL.32.002049},
      Institution              = {Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. Robert.Huber@Physik.Uni-Muenchen.de},
      ISSN                     = {0146-9592},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-14-2049}
    }
  • Huber, Robert and Srinivasan, Vivek J and Adler, Desmond C and Gorczynska, I and Fujimoto, James G: Fourier Domain Mode Locking (FDML) in the non-zero dispersion regime: A laser for ultrahigh-speed retinal OCT imaging at 236kHz line rate. in Conference on Lasers and Electro-Optics, 2007, CLEO 2007, 2007
    BibTeX Link
    @InProceedings{HU_2007_Huber_b,
      Title                    = {{Fourier Domain Mode Locking (FDML) in the non-zero dispersion regime: A laser for ultrahigh-speed retinal OCT imaging at 236kHz line rate}},
      Author                   = {Huber, Robert and Srinivasan, Vivek J and Adler, Desmond C and Gorczynska, I and Fujimoto, James G},
      Booktitle                = {Conference on Lasers and Electro-Optics, 2007, CLEO 2007},
      Year                     = {2007},
    
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=CLEO-2007-CThAA5}
    }
  • Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther: Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues Laser Ablation and its Applications. no. 129, pp. 231-280, Springer Berlin / Heidelberg, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther},
       title = {Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues
    Laser Ablation and its Applications},
       editor = {Phipps, Claude},
       series = {Springer Series in Optical Sciences},
       publisher = {Springer Berlin / Heidelberg},
       volume = {129},
       pages = {231-280},
       keywords = {Physics and Astronomy},
       year = {2007}
    }
  • Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther: Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues. in Laser Ablation and Its Applications, no. 129, pp. 231-280, Springer US, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, Alfred and Noack, Joachim and Hüttman, Gereon and Paltauf, Günther},
       title = {Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues},
       booktitle = {Laser Ablation and Its Applications},
       editor = {Phipps, Claude},
       series = {Springer Series in Optical Sciences},
       publisher = {Springer US},
       volume = {129},
       chapter = {10},
       pages = {231-280},
       year = {2007}
    }
    
  • Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.: Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography. Curr Eye Res, no. 32, pp. 171-176, 2007
    BibTeX
    @article{Müller,
       author = {Müller, M. and Winter, C. and Hüttmann, G. and Lacqua, H. and Hoerauf, H.},
       title = {Evaluation of Cyclophotocoagulation Effects with 1310-nm Contact Optical Coherence Tomography},
       journal = {Curr Eye Res},
       volume = {32},
       pages = {171-176},
       year = {2007}
    }
  • Framme, C and Brinkmann, R: Die Selektive Retina Therapie (SRT). Der Augenspiegel, no. 53G1396, 2007
    BibTeX
    @article{Framme,
       author = {Framme, C and Brinkmann, R},
       title = {Die Selektive Retina Therapie (SRT)},
       journal = {Der Augenspiegel},
       volume = {53G1396},
       number = {11},
       year = {2007}
    }
  • Hüttmann, G.: Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie. Der Gynäkologe, no. 40, pp. 372, 2007
    BibTeX
    @article{Hüttmann2007,
       author = {Hüttmann, G.},
       title = {Der Weg in die Zukunft: In-vivo-Pathologie durch Laserscan-Mikroskopie},
       journal = {Der Gynäkologe},
       volume = {40},
       number = {5},
       pages = {372},
       year = {2007}
    }
  • Adler, Desmond C and Stenger, Jens and Gorczynska, Iwona and Lie, Henry and Hensick, Teri and Spronk, Ron and Wolohojian, Stephan and Khandekar, Narayan and Jiang, James Y and Barry, Scott and Cable, Alex E and Huber, Robert and Fujimoto, James G: Comparison of three-dimensional optical coherence tomography and high resolution photography for art conservation studies. Optics Express, no. 15, pp. 15972 -15986, nov,, 2007
    BibTeX Link
    @Article{HU_2007_Adler_b,
      Title                    = {{Comparison of three-dimensional optical coherence tomography and high resolution photography for art conservation studies}},
      Author                   = {Adler, Desmond C and Stenger, Jens and Gorczynska, Iwona and Lie, Henry and Hensick, Teri and Spronk, Ron and Wolohojian, Stephan and Khandekar, Narayan and Jiang, James Y and Barry, Scott and Cable, Alex E and Huber, Robert and Fujimoto, James G},
      Journal                  = {Optics Express},
      Year                     = {2007},
    
      Month                    = nov,
      Number                   = {24},
      Pages                    = {15972 -- 15986 },
      Volume                   = {15},
    
      Doi                      = {10.1364/OE.15.015972},
      ISSN                     = {1094-4087},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-24-15972}
    }
  • Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.: Combining Optical Coherence Tomographie (OCT) with an operating microscope. in Medical Engineering, pp. pp 343-348, Springer, Berlin, Heidelberg, New York, 2007
    BibTeX
    @inbook{Lankenau,
       author = {Lankenau, E. and Klinger, D. and Winter, C. and Malik, A. and Müller, H.H. and Oelckers, S. and Pau, H.W. and Just, T. and Hüttmann, G.},
       title = {Combining Optical Coherence Tomographie (OCT) with an operating microscope},
       booktitle = {Medical Engineering},
       editor = {Buzug, T.M. and Holz, D. and Weber, S.  and Bongartz, J. and Kohl-Bareise, M. and Hartmann, U.},
       publisher = {Springer, Berlin, Heidelberg, New York},
       pages = {pp 343-348},
       year = {2007}
    }
  • Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.: Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif, no. 40, pp. 422-30, 2007
    BibTeX
    @article{Rahmanzadeh,
       author = {Rahmanzadeh, R. and Huttmann, G. and Gerdes, J. and Scholzen, T.},
       title = {Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis},
       journal = {Cell Prolif},
       volume = {40},
       number = {3},
       pages = {422-30},
       note = {Rahmanzadeh, R
    Huttmann, G
    Gerdes, J
    Scholzen, T
    England
    Cell Prolif. 2007 Jun;40(3):422-30.},
       abstract = {OBJECTIVES: Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Recently, we proposed a function of pKi-67 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi-67 in this process by photochemical inhibition using chromophore-assisted light inactivation (CALI). MATERIALS AND METHODS: Anti-pKi-67 antibodies were labelled with the fluorochrome fluorescein 5(6)-isothiocyanate and were irradiated after binding to their target protein. RESULTS: Performing CALI in vitro on cell lysates led to specific cross-linking of pKi-67. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross-link formation, indicating a close spatial proximity of UBF and pKi-67. CALI in living cells, using micro-injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi-67-CALI resulted in dramatic inhibition of RNA polymerase I-dependent nucleolar rRNA synthesis, whereas RNA polymerase II-dependent nucleoplasmic RNA synthesis remained almost unaltered. CONCLUSIONS: Our data presented here argue for a crucial role of pKi-67 in RNA polymerase I-dependent nucleolar rRNA synthesis.},
       keywords = {Antibodies, Antinuclear
    Antibodies, Monoclonal
    Cell Division/physiology
    Cell Nucleolus/physiology
    Fluorescein-5-isothiocyanate
    Fluorescent Dyes
    HeLa Cells
    Humans
    Ki-67 Antigen/*genetics/*metabolism
    Photochemistry
    RNA Polymerase I/metabolism
    RNA, Ribosomal/*biosynthesis},
       year = {2007}
    }
  • Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.: Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues. in Handai Nanophotonics, no. Volume 3, pp. 273-286, Elsevier, 2007
    BibTeX
    @inbook{Vogel2007,
       author = {Vogel, A. and Noack, J. and Hüttmann, G. and Linz, N. and Freidank, S. and Paltauf, G.},
       title = {Chapter 18 Femtosecond laser nanosurgery of biological cells and tissues},
       booktitle = {Handai Nanophotonics},
       editor = {Hiroshi Masuhara, Satoshi Kawata and Fumio, Tokunaga},
       publisher = {Elsevier},
       volume = {Volume 3},
       pages = {273-286},
       year = {2007}
    }
  • Brinkmann, R. and Birngruber, R.: [Selective Retina Therapy (SRT)]. Z Med Phys, no. 17, pp. 6-22, 2007
    BibTeX
    @article{Brinkmann,
       author = {Brinkmann, R. and Birngruber, R.},
       title = {[Selective Retina Therapy (SRT)]},
       journal = {Z Med Phys},
       volume = {17},
       number = {1},
       pages = {6-22},
       note = {Brinkmann, Ralf
    Birngruber, Reginald
    English Abstract
    Review
    Germany
    Z Med Phys. 2007;17(1):6-22.},
       abstract = {Selective Retina Therapy (SRT) is a new and very gentle laser method developed at the Medical Laser Center Lubeck. It is currently investigated clinically in order to treat retinal disorders associated with a decreased function of the retinal pigment epithelium (RPE). SRT is designed to selectively effect the RPE while sparing the neural retina and the photoreceptors as well as the chorioidea. Aim of the therapy is the rejuvenation of the RPE in the treated areas, which should ideally lead to a long term metabolic increase at the chorio-retinal junction. In contrast to conventional laser photocoagulation, which is associated with a complete thermal necrosis of the treated site, SRT completely retains full vision. This paper reviews the methods and mechanisms behind selective RPE effects and reports the first clinical results. An online dosimetry technique to visualize the ophthalmoscopically invisible effects is introduced.},
       keywords = {Humans
    Laser Therapy
    Pigment Epithelium of Eye/pathology
    Regeneration
    Retinal Diseases/pathology/surgery/ therapy},
       year = {2007}
    }