Nichtlineare biomolekulare Bildgebung

Die nichtlineare optische Mikroskopie ist eine leistungsstarke Technik für die Biomolekularwissenschaft, die es ermöglicht, biochemische Prozesse auf zellulärer Ebene besser zu verstehen. Das Hauptziel ist es, nicht nur die Morphologie, sondern auch die chemische Zusammensetzung einer Probe zu identifizieren.

Wir arbeiten derzeit an zwei Ansätzen um optisch Kontrast zu erzeugen:

  • Stimulierte Raman-Streuung (SRS) - Mikroskopie
  • Zweiphotonen-Fluoreszenzmikroskopie (TPEF)

 

 

 

Zeitcodierte (TICO) Ramanspektroskopie und Mikroskopie

Der TICO-Raman-Ansatz verwendet einen wellenlängenabtastenden Probe-Laser, um spektrale Datenpunkte nacheinander aufzunehmen. Breitbandig und mit einer spektralen Auflösung, die nur durch die Laserlinienbreite begrenzt ist, verfügt dieses System über weitreichende spektroskopische Möglichkeiten, die sich auch für schnelle Abbildungsanwendungen anbietet.

Das TICO-Raman-Konzept

Der zeitlich kodierte (TICO)-Ramanansatz verwendet einen schnell wellenlängenabtastenden FDML-Laser in Kombination mit einem schmalbandigen, aktiv modulierten Pumplaser, um die Ramanspektren direkt zeitlich zu kodieren und zu messen. Der FDML-Probe-Laser ändert seine Wellenlänge kontinuierlich und wiederholend mit der Zeit. Dies ist in Abbildung 1A als regenbogenfarbene Linie dargestellt. Die blaue Linie zeigt den einfarbigen schmalbandigen Pumplaser. Man erkennt deutlich, dass sich die Energiedifferenz zwischen diesen beiden Lichtquellen im Laufe der Zeit ändert. Daher werden bei unterschiedlichem Timing verschiedene Raman-Übergänge abgetastet (Abbildung 1B). Wenn die Energiedifferenz mit einem Ramanübergang übereinstimmt, werden Pumpphotonen in Photonen der Probe-Wellenlänge umgewandelt und die FDML-Laserintensität steigt an dieser Stelle an. Diese Intensitätsänderung kann mit einem schnellen Photodetektor gemessen werden (Abbildung 1C).

Um hohe Signalpegel zu erreichen, wird der Pumplaser nicht durchgängig betrieben, sondern zu kurzen Pulsen moduliert (Abbildung 1D). Um alle möglichen Ramanübergänge zu untersuchen, werden diese Pulse gegenüber dem Start eines FDML-Laser-Sweeps für jeden neuen Sweep immer weiter verzögert. Die Intensitätsänderungen können mit der bekannten Zeit-zu-Wellenlängen-Kodierung direkt auf ein Raman-Spektrum abgebildet werden.

Abbildung 1: Konzept der zeitlich kodierten (TICO)-Ramanspektroskopie
Konzept der zeitlich kodierten (TICO)-Ramanspektroskopie

Zweiphotonen-Fluoreszenzmikroskopie (TPEF)

a) TPEF-Bildgebung b) Dauer des Anregungspulses
TPEF-Bild eines Pflanzenblattes

In den letzten Jahren hat sich die Multiphotonenmikroskopie in der biomedizinischen Bildgebung zu einer Möglichkeit entwickelt, dünne Schichten in streuendem Gewebe sehr genau abzubilden. Aktuelle Multiphotonenmikroskope verwenden komplizierte Lasersysteme. Daher untersuchen wir Alternativkonzepte. Der Anregungslaser unseres Systems ist ein vollständig faserbasierter, gepulster Laser mit Sub-Nanosekunden-Pulsen. Dies macht das Gesamtkonzept sehr robust, zuverlässig und wartungsfrei. Ein wesentlicher Vorteil des Systems ist die direkte Synchronisation der Anregungspulse mit der Datenerfassung, die neue bildgebende Verfahren ermöglicht.

Zweiphotonen-Einzelpuls-Fluoreszenzlebenszeitbildgebung (SP-FLIM)

SP-FLIM-Aufnahmen eines Maiglöckchenstängels (Convallaria majalis).

Eine der neuen Bildgebungsverfahren, die mit unserer neuen Laserquelle möglich sind, ist die Einzelpuls-Zweiphotonen-Fluoreszenzlebenszeitmikroskopie (SP-FLIM). Zweiphotonen-FLIM ist ein chemisch spezifisches 3-D-Bildgebungsverfahren, bei dem die Lebensdauer eines Fluoreszenzabfalls wertvolle Informationen über die chemische Zusammensetzung der untersuchten Probe liefert. Aufgrund unserer längeren Pulslänge im Vergleich zu herkömmlichen Ti:Sa-Lasern werden mehr Fluoreszenz-Photonen pro Puls erzeugt, so dass wir die Lebensdauer mit nur einem einzigen Anregungspuls bestimmen können. Mit dieser Methode erreichen wir sehr schnelle Aufnahmegeschwindigkeiten für FLIM-Bildern mit Pixelraten mit bis zu 1MHz.

related Publications

2019

  • Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R;Popp, J;Limpert, J;Karpf, S and Huber, R: Virtual HE histology by fiber-based picosecond two-photon microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIX Proc. SPIE,10882, pp. 108822F, International Society for Optics and Photonics, 2019
    BibTeX Link
    @inproceedings{Kolb2019/2,
       author = {Kolb, J P;Weng, D;Hakert, H;Eibl, M;Draxinger, W;Meyer, T;Gottschall, T;Brinkmann, R;Birngruber, R;Popp, J;Limpert, J;Karpf, S and Huber, R},
       title = {Virtual HE histology by fiber-based picosecond two-photon microscopy},
       booktitle = {Multiphoton Microscopy in the Biomedical Sciences XIX} {Proc. SPIE,10882},
       editor = {Ammasi Periasamy; Peter T. C. So; Karsten König},
       publisher = { International Society for Optics and Photonics},
       year = {2019},
       pages = {108822F},
    keywords = {AG-Huber_NL},
       
    doi = {10.1117/12.2507866},
       type = {Conference Proceedings}
    }
    

2018

  • Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S: Wavelength agile multi-photon microscopy with a fiber amplified diode laser. Biomedical Optics Express 9(12), pp. 6273-6282, 2018
    BibTeX Link
    @article{Eibl2018,
    author = { Eibl ,M; Weng, D; Hakert, H; Kolb, J P; Pfeiffer, T; Hundt, J E; Huber, R and Karpf, S},
    title = {Wavelength agile multi-photon microscopy with a fiber amplified diode laser},
    journal = {Biomedical Optics Express} {9(12)},
    keywords = {AG-Huber_NL},
    year = {2018},
    pages = {6273--6282},
    doi = {10.1364/BOE.9.006273},
    }
    

2017

  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert: Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection. in Proc. SPIE, no. 10069, pp. 100691F-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Huber, Robert},
       title = {Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection},
    booktitle = {Proc. SPIE },
       volume = {10069},
       pages = {100691F-6},
       doi= {10.1117/12.2250831},
      year={2017},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    
  • Hakert, H; Eibl, M; Karpf, S and Huber, R: Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition. in Proc. SPIE 10414, pp. 1041408-1041408-7, 2017
    BibTeX Link
    @inproceedings{Hakert2017,
       author = {Hakert, H; Eibl, M; Karpf, S and Huber, R},
       title = {Sparse-sampling with time-encoded (TICO) stimulated Raman scattering for fast image acquisition},
    booktitle = {Proc. SPIE} {10414},
    Year={2017},
       
       pages = {1041408-1041408-7},
       url = {http://dx.doi.org/10.1117/12.2287947},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    
  • Eibl, M; Karpf, S; Hakert, H; Weng, D; Pfeiffer, T; Kolb, J P and Huber, R: Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup. in Proc. SPIE 10414, pp. 1041403-1041403-7, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, M; Karpf, S; Hakert, H; Weng, D; Pfeiffer, T; Kolb, J P and Huber, R},
       title = {Single pulse two-photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate and an all fiber based setup},
      booktitle = {Proc. SPIE} {10414},
      
      year = {2017},
    keywords = {AG-Huber_NL},
       pages = {1041403-1041403-7},
       url = {http://dx.doi.org/10.1117/12.2286035},
       type = {Conference Proceedings}
    }
    
  • Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber: Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate. Biomedical Optics Express, no. 8, pp. 3132-3142, OSA, Jul, 2017
    BibTeX Link
    @article{HU_2017_Eibla_BOE,
    author = {Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber},
    journal = {Biomedical Optics Express},
    number = {7},
    pages = {3132--3142},
    publisher = {OSA},
    title = {Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate},
    volume = {8},
    month = {Jul},
    year = {2017},
    keywords = {AG-Huber_NL},
    doi = {10.1364/BOE.8.003132}
    }
    
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Shot-Noise Limited Time-Encoded Raman Spectroscopy. Journal of Spectroscopy, no. 2017, pp. 1-6, 2017
    BibTeX Link
    @article{Karpf2017,
       author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
       title = {Shot-Noise Limited Time-Encoded Raman Spectroscopy},
       journal = {Journal of Spectroscopy},
       volume = {2017},
       pages = {1-6},
       url = {https://doi.org/10.1155/2017/9253475},
       year = {2017},
    keywords = {AG-Huber_NL},
       type = {Journal Article}
    }
    
  • Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert: Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging. in Proc. SPIE, no. 10083, pp. 100831C-6, 2017
    BibTeX Link
    @inproceedings{Eibl2017,
       author = {Eibl, Matthias and Karpf, Sebastian and Hakert, Hubertus and Weng, Daniel and Blömker, Torben and Huber, Robert},
       title = {Pulse-to-pulse wavelength switching of diode based fiber laser for multi-color multi-photon imaging},
    booktitle = {Proc. SPIE },
       volume = {10083},
       pages = {100831C-6},
       year= {2017},
       doi= {10.1117/12.2251965},
    keywords = {AG-Huber_NL},
       type = {Conference Proceedings}
    }
    
  • Eibl, M; Karpf, S; Hakert, H; Blömker, T; Kolb, J P; Jirauschek, C and Huber, R: Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification. Opt Lett 42(21), pp. 4406-4409, OSA, Nov, 2017
    BibTeX Link
    @article{2017Eibl,
    author = {Eibl, M; Karpf, S; Hakert, H; Bl\"{o}mker, T; Kolb, J P; Jirauschek, C and Huber, R},
    journal = {Opt Lett} {42(21)},
    keywords = {NLI, TPEF, Multiphoton, AG-Huber_NL},
    
    pages = {4406--4409},
    publisher = {OSA},
    title = {Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification},
    
    month = {Nov},
    year = {2017},
    doi = {10.1364/OL.42.004406},
    }
    
  • Klein, Thomas and Huber, Robert: High-speed OCT light sources and systems [Invited]. Biomedical Optics Express, no. 8, pp. 828-859, 2017
    BibTeX Link
    @article{Klein2017,
       author = {Klein, Thomas and Huber, Robert},
       title = {High-speed OCT light sources and systems [Invited]},
       journal = {Biomedical Optics Express},
       volume = {8},
       number = {2},
       pages = {828-859},
       abstract = {Imaging speed is one of the most important parameters that define the performance of optical coherence tomography (OCT) systems. During the last two decades, OCT speed has increased by over three orders of magnitude. New developments in wavelength-swept lasers have repeatedly been crucial for this development. In this review, we discuss the historical evolution and current state of the art of high-speed OCT systems, with focus on wavelength swept light sources and swept source OCT systems.},
       keywords = {AG-Huber_FDML, AG-Huber_OCT, AG-Huber_NL},
       DOI = {10.1364/BOE.8.000828},
       year = {2017},
       type = {Journal Article}
    }
    

2016

  • Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert: Two-photon microscopy using fiber-based nanosecond excitation. Biomedical Optics Express, no. 7, pp. 2432-2440, 2016
    BibTeX Link
    @article{Karpf2016,
       author = {Karpf, Sebastian and Eibl, Matthias and Sauer, Benjamin and Reinholz, Fred and Hüttmann, Gereon and Huber, Robert},
       title = {Two-photon microscopy using fiber-based nanosecond excitation},
       journal = {Biomedical Optics Express},
       volume = {7},
       number = {7},
       pages = {2432-2440},
       abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
       keywords = {AG-Huber_NL},
       DOI = {10.1364/BOE.7.002432},
       year = {2016},
       type = {Journal Article}
    }
    

2015

  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers. in Proc. SPIE, no. 9541, pp. 95410F-95410F-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_b,
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = { Time-encoded Raman scattering (TICO-Raman) with Fourier domain mode locked (FDML) lasers },
    booktitle = {Proc. SPIE },
    volume = {9541},
    type = {Conference Proceedings},
    pages = {95410F-95410F-6},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183859}
    }
  • Karpf, Sebastian and Eibl, Matthias and Huber, Robert: Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser. in Proc. SPIE, no. 9536, pp. 953616-953616-5, 2015
    BibTeX Link
    @inproceedings{HU_2015_Karpf_c,
    author = {Karpf, Sebastian and Eibl, Matthias and Huber, Robert},
    title = { Nanosecond two-photon excitation fluorescence imaging with a multi color fiber MOPA laser },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953616-953616-5},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183854}
    }
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Hyperspectral stimulated Raman microscopy with two fiber laser sources. in Proc. SPIE, no. 9536, pp. 953604-953604-6, 2015
    BibTeX Link
    @inproceedings{HU_2015_Eibl_a,
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    title = {Hyperspectral stimulated Raman microscopy with two fiber laser sources },
    booktitle = {Proc. SPIE },
    type = {Conference Proceedings},
    volume = {9536},
    pages = {953604-953604-6},
    year = {2015},
    keywords = {AG-Huber_NL},
    doi = {10.1117/12.2183822}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy. Nature Communications, no. 6, pp. 784 1-6;, 2015
    BibTeX Link
    @Article{HU_2015_Karpf_a,
      Title                    = {A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy},
      Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
      Journal                  = {Nature Communications},
      Year                     = {2015},
      Volume = {6},
      pages = {6784 1--6};
    keywords = {AG-Huber_NL},
      Doi                      = {10.1038/ncomms7784}
    }
    

2014

  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Time-Encoded Raman: Fiber-based, hyperspectral, broadband stimulated Raman microscopy. ArXiv e-prints, 2014
    BibTeX Link
    @Article{HU_2014_Karpf_a,
      Title                    = {{Time-Encoded Raman: Fiber-based, hyperspectral, broadband stimulated Raman microscopy}},
      Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
      journal = {ArXiv e-prints},
      Year                     = {2014},
      Archiveprefix            = {arXiv},
      Arxivid                  = {1405.4181},
      Eprint                   = {1405.4181},
    keywords = {AG-Huber_NL},
      Url                      = {http://arxiv.org/abs/1405.4181}
    }
  • Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert: Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. 1-2, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Karpf_b,
    abstract = {A hyperspectral stimulated Raman microscopy system using rapidly wavelength swept lasers is presented. Imaging of biological samples with shot noise limited detection is demonstrated with the fiber based setup.},
    author = {Karpf, Sebastian and Eibl, Matthias and Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_SI.2014.SM3P.3},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman microscopy,Scattering,stimulated Raman,tunable, AG-Huber_NL},
    pages = {1--2},
    publisher = {OSA},
    title = {{Hyperspectral Stimulated Raman Microscopy with Fiber-based, Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_SI-2014-SM3P.3},
    year = { 2014}
    }
  • Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers. in CLEO: 2014, pp. ATu3P.4 1-3, OSA, 2014
    BibTeX Link Link
    @inproceedings{HU_2014_Eibl_a,
    abstract = {A fast all fiber based setup for stimulated Raman spectroscopy with a rapidly wavelength swept cw-laser is presented. It enables flexible acquisition of broadband (750 cm-1 to 3150 cm-1) spectra with high resolution (0.5 cm-1).},
    author = {Eibl, Matthias and Karpf, Sebastian and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2014},
    doi = {10.1364/CLEO_AT.2014.ATu3P.4},
    isbn = {978-1-55752-999-2},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,stimulated Raman,tunable,AG-Huber_NL},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{Broadband, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept cw-Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO\_AT-2014-ATu3P.4},
    year = { 2014}
    }

2013

  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: New High Resolution SRS with ultra broadband spectral coverage. in 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference, pp. PD_A_8 1-3, Optical Society of America, Munich, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_a,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    address = {Munich},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference},
    pages = {PD_A_8 1--3},
    publisher = {Optical Society of America},
    doi={10.1109/CLEOE-IQEC.2013.6801995},
    title = {{FDML Raman: New High Resolution SRS with ultra broadband spectral coverage}},
    url = {https://doi.org/10.1109/CLEOE-IQEC.2013.6801995},
    keywords = {AG-Huber_NL},
    year = { 2013}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers. in CLEO: 2013, pp. ATu3P.4 1-3, OSA, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_b,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2013},
    doi = {10.1364/CLEO_SI.2013.CTu2H.5},
    isbn = {978-1-55752-972-5},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,fiber,stimulated Raman,AG-Huber_NL},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_SI-2013-CTu2H.5},
    year = { 2013}
    }

2009

  • Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P: A 75 MHz light source for femtosecond stimulated raman microscopy. Optics express, no. 17, pp. 18612-20, t,, 2009
    BibTeX Link Link
    @Article{HU_2009_Ploetz_a,
      Title                    = {{A 75 MHz light source for femtosecond stimulated raman microscopy}},
      Author                   = {Ploetz, E and Marx, B and Klein, T and Huber, R and Gilch, P},
      Journal                  = {Optics express},
      Year                     = {2009},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {18612--20},
      Volume                   = {17},
    
      Doi                      = {10.1364/OE.17.018612},
      Institution              = {Lehrstuhl f\"{u}r BioMolekulare Optik and Center of Integrated Protein Science Munich Fakult\"{a}t f\"{u}r Physik, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen Oettingenstr 67, D-80538 M\"{u}nchen, Germany.},
      ISSN                     = {1094-4087},
    keywords = {AG-Huber_NL},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-17-21-18612}
    }