Publikationen 2013 - 2011

2013

  • Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs: Ultrahigh-speed intravascular optical coherence tomography imaging at 3200 frames per second. in European Conferences on Biomedical Optics, pp. 88020O 1-2, International Society for Optics and Photonics, n,, 2013
    BibTeX Link Link
    @InProceedings{HU_2013_Wang_b,
      Title                    = {{Ultrahigh-speed intravascular optical coherence tomography imaging at 3200 frames per second}},
      Author                   = {Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2013},
      Editor                   = {Bouma, Brett E and Leitgeb, Rainer A},
      Month                    = jun,
      Pages                    = {88020O 1--2 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.2032723},
    keywords = {AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2032723}
    }
  • Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.: Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress. Invest Ophthalmol Vis Sci, 2013
    BibTeX Link
    @article{Miura2013,
       author = {Miura, Y. and Huettmann, G. and Orzekowsky-Schroeder, R. and Steven, P. and Szaszak, M. and Koop, N. and Brinkmann, R.},
       title = {Two-Photon Microscopy and Fluorescence Lifetime Imaging of Retinal Pigment Epithelial Cells under Oxidative Stress},
       journal = {Invest Ophthalmol Vis Sci},
       note = {Miura, Yoko
    Huettmann, Gereon
    Orzekowsky-Schroeder, Regina
    Steven, Philipp
    Szaszak, Marta
    Koop, Norbert
    Brinkmann, Ralf
    ENG
    2013/04/06 06:00
    Invest Ophthalmol Vis Sci. 2013 Apr 4. pii: iovs.13-11808v1. doi: 10.1167/iovs.13-11808.},
       abstract = {PURPOSE: The aim of this study was to investigate the autofluorescence (AF) of the RPE with two-photon microscopy (TPM) and fluorescence lifetime imaging (FLIM) under normal and oxidative stress conditions. METHODS: Porcine RPE-choroid explants were used for investigation. The RPE-choroid tissue was preserved in a perfusion organ culture system. Oxidative stress was induced by laser photocoagulation with frequency-doubled Nd:YAG laser (532 nm) and by exposure to different concentrations (0, 1, 10 mM) of ferrous sulfate (FeSO4) for 1 hr. At indicated time points after exposure, the tissue was examined with TPM and FLIM. Intracellular reactive oxygen species around the photocoagulation lesion were detected with chloromethyl-2'7'-dichlorofluorescein diacetate (CM-H2DCFDA). Melanosomes were isolated from RPE cells and its fluorescence properties were investigated under normal and oxidized conditions. RESULTS: Under normal condition, AF in RPE cells with TPM is mostly originated from melanosomes, which has a very short fluorescence lifetime (FLT) (mean=117 ps). Under oxidative stress induced by laser irradiation and FeSO4 exposure, bright granular AF appears inside and around RPE cells, whose FLT is significantly longer (mean=1388 ps) than the FLT of the melanosome-AF. Excitation and emission peaks are found at 710-750 nm and 450-500 nm, respectively. Oxidative stress increases the fluorescence intensity of the melanosomes but does not change their FLT. CONCLUSION: TPM reveals acute oxidative stress-induced bright AF granules inside and around RPE cells which can be clearly discriminated from melanosomes by FLIM. TPM combined with FLIM is a useful tool of live-cell analysis to investigate functional alterations of the RPE.},
       year = {2013}
    }
    
  • Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R: Two-photon Microscopy and Fluorescence Lifetime Analysis of Lipid Peroxidation Product in Photoreceptor Outer Segment and in Retinal Pigment Epithelial Cell. ARVO Meeting Abstracts, March 26, 2012, 2013
    BibTeX Link
    @misc{Miura2013,
       author = {Miura, Y and Huettmann, G and Orzekowsky-Schroeder, R and Steven, P and Szaszák, M and Koop, N and Brinkmann, R },
       title = {Two-photon Microscopy and Fluorescence Lifetime Analysis of Lipid Peroxidation Product in Photoreceptor Outer Segment and in Retinal Pigment Epithelial Cell},
       publisher = {ARVO Meeting Abstracts},
       month = {March 26, 2012 },
       url = {http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=57630548-893d-4e45-9ddc-b6f547dd4ff0&cKey=d08a30bc-fe98-40a2-8a1c-1b171e4becd3&mKey=f0fce029-9bf8-4e7c-b48e-9ff7711d4a0e},
       year = {2013},
       type = {Poster}
    }
    
  • Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon: Towards microscopic resolution in holoscopy. pp. 85711O-85711O, 2013
    BibTeX Link Link
    @article{Franke2013,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Lührs, Christian and Koch, Peter and Wollenzin, Jörn and Hüttmann, Gereon},
       title = {Towards microscopic resolution in holoscopy},
       pages = {85711O-85711O},
       note = {10.1117/12.2006806},
       abstract = {Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.},
       DOI = {10.1117/12.2006806},
       url = {http://dx.doi.org/10.1117/12.2006806},
       year = {2013},
       type = {Journal Article}
    }
    
  • Brinkmann, Ralf and Iwami, Hisashi and Pruessner, Joachim and Danicke, Veit and Miura, Yoko: Temperature-dependent response of retinal pigment epithelial cells to laser irradiation. Invest. Ophthalmol. Vis. Sci., no. 54, pp. 1809-, 2013
    BibTeX Link
    @article{Brinkmann2013,
       author = {Brinkmann, Ralf and Iwami, Hisashi and Pruessner, Joachim and Danicke, Veit and Miura, Yoko},
       title = {Temperature-dependent response of retinal pigment epithelial cells to laser irradiation},
       journal = {Invest. Ophthalmol. Vis. Sci.},
       volume = {54},
       number = {6},
       pages = {1809-},
       abstract = {PurposeSublethal thermal therapy of the retinal pigment epithelium (RPE) is discussed as a new prophylactic therapy for age-related macular degeneration. However, temperature-dependent RPE cell effects have not been well elucidated. We investigated the biochemical responses of RPE cells following sublethal to lethal thermal laser irradiation. MethodsPorcine RPE cells cultured in a dish (33mm) were heated with a Thulium laser (1.92{micro}m, 1-20W, 10s) over a spot of 3mm. Temperatures during irradiation were measured with thermocouples. Cell viability was examined using annexin-V, ethidium homodimer III and Hoechst 33342 for detecting apoptotic, necrotic and living cell, respectively, by using fluorescence microscopy for localization and flow cytometry for quantification. Secretion of vascular endothelial growth factor (VEGF) for 6h following irradiation on different temperatures was assessed with Elisa assay. In order to examine a protective effect of sublethal hyperthremia, the cells were heated up to 45C 24h prior to the exposure of 2 mM hydroxyl peroxide (H2O2) for 5 h. The involvement of TRPV (Transient Receptor Potential Vanilloid)-1 receptor, which is activated with temperatures > 43C, was investigated by adding capsazepin, a TRPV-1 inhibitor, before irradiation. ResultsCell apoptosis and necrosis was observed 24 h after irradiation with a central peak temperature [&ge;]52C. Fluorescence microscopy revealed apoptotic cells around the central necrotic area. VEGF secretion for 6h after irradiation was significantly increased at peak temperatures between 40 and 52C in a temperature dependent manner (max. 110%, p<0.05), whereas the total secretion decreases with temperatures > 52C. Pre-irradiation onto 45C significantly reduced H2O2-induced cell death after 5h compared to non-heated cells (total cell death: 15.6% to 10.2%, necrosis: 6% to 4 %, early apoptosis: 5.1% to 3.6%; p<0.01). These effects were not observed in the existence of capsazepin during laser irradiation. ConclusionsThe number of apoptotic and necrotic RPE cells increase at least over 24h following thermal laser irradiation. Sublethal temperatures between 40 and 52C seem to induce various cellular responses as VEGF secretion, which might be related to the protective effect against oxidative stress. Results with capsazepin suggest that TRPV-1 channel activation by hyperthermia is essential to exert this protective effect.},
       url = {http://abstracts.iovs.org/cgi/content/abstract/54/6/1809},
       year = {2013},
       type = {Journal Article}
    }
    
  • Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon: Stray light rejection by structured illumination. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX
    @inproceedings{Fink2013,
       author = {Fink, Peer K and Hillmann, Dierck and Franke, Gesa L and Ramm, Dirk and Koch, P and Hüttmann, Gereon},
       title = {Stray light rejection by structured illumination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    year = { 2013}
    }
    
  • Lauterborn, Werner and Vogel, Alfred: Shock Wave Emission by Laser Generated Bubbles. in Bubble Dynamics and Shock Waves, pp. 67-103, 2013
    BibTeX
    @inbook{Lauterborn2013,
       author = {Lauterborn, Werner and Vogel, Alfred},
       title = {Shock Wave Emission by Laser Generated Bubbles},
       booktitle = {Bubble Dynamics and Shock Waves},
       pages = {67-103},
       year = {2013}
    }
    
  • Koop, N: Schreib‘ mal wieder! Neue Laser-Markierungsverfahren und spezielle Mikrobearbeitungen. GIT Labor-Fachzeitschrift, 2013
    BibTeX
    @article{Koop2013,
       author = {Koop, N},
       title = {Schreib‘ mal wieder! Neue Laser-Markierungsverfahren und spezielle Mikrobearbeitungen},
       journal = {GIT Labor-Fachzeitschrift},
       number = {9},
       year = {2013},
       type = {Journal Article}
    }
    
  • Torzicky, Teresa and Marschall, Sebastian and Pircher, Michael and Baumann, Bernhard and Bonesi, Marco and Zotter, Stefan and Götzinger, Erich and Trasischker, Wolfgang and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Huber, Robert and Andersen, Peter and Hitzenberger, Christoph K: Retinal polarization-sensitive optical coherence tomography at 1060 nm with 350 kHz A-scan rate using an Fourier domain mode locked laser. Journal of biomedical optics, no. 18, pp. 26008-8, b,, 2013
    BibTeX Link Link
    @Article{HU_2013_Torzicky_a,
      Title                    = {{Retinal polarization-sensitive optical coherence tomography at 1060 nm with 350 kHz A-scan rate using an Fourier domain mode locked laser}},
      Author                   = {Torzicky, Teresa and Marschall, Sebastian and Pircher, Michael and Baumann, Bernhard and Bonesi, Marco and Zotter, Stefan and G\"{o}tzinger, Erich and Trasischker, Wolfgang and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin and Huber, Robert and Andersen, Peter and Hitzenberger, Christoph K},
      Journal                  = {Journal of biomedical optics},
      Year                    = {2013},
      Month                    = feb,
      Number                   = {2},
      Pages                    = {26008--8},
      Volume                   = {18},
      Doi                      = {10.1117/1.JBO.18.2.026008},
      ISSN                     = {1560-2281},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1568209}
    }
    
  • Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon: Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX Link
    @inproceedings{Fleischhauer2013,
       author = {Fleischhauer, Felix and Schulz-Hildebrandt, Hinnerk and Bonin, Tim and Hüttmann, Gereon},
       title = {Polarization-sensitive optical coherence tomography on different tissues samples for tumor discrimination},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    url = { https://pdfs.semanticscholar.org/a581/a18366acff021e12dcc090b40890ea70dcb8.pdf},
     year = { 2013}
    }
    
  • Eigenwillig, Christoph M and Wieser, Wolfgang and Todor, Sebastian and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert: Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers. Nature communications, no. 4, pp. 1848-1855, n,, 2013
    BibTeX Link Link
    @Article{HU_2013_Eigenwillig_b,
      Title                    = {{Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers}},
      Author                   = {Eigenwillig, Christoph M and Wieser, Wolfgang and Todor, Sebastian and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert},
      Journal                  = {Nature communications},
      Year                     = {2013},
      Month                    = jan,
      Pages                    = {1848--1855},
      Volume                   = {4},
      Doi                      = {10.1038/ncomms2870},
      ISSN                     = {2041-1723},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.nature.com/ncomms/journal/v4/n5/abs/ncomms2870.html}
    }
  • Eigenwillig, Christoph M and Todor, Sebastian and Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert: Picosecond pulses from a Fourier domain mode locked (FDML) laser. in 2013 Conference on Lasers &amp; Electro-Optics Europe &amp; International Quantum Electronics Conference CLEO EUROPE/IQEC, pp. 1-1, IEEE, y,, 2013
    BibTeX Link Link
    @InProceedings{HU_2013_Eigenwillig_a,
      Title                    = {{Picosecond pulses from a Fourier domain mode locked (FDML) laser}},
      Author                   = {Eigenwillig, Christoph M and Todor, Sebastian and Wieser, Wolfgang and Biedermann, Benjamin R and Klein, Thomas and Jirauschek, Christian and Huber, Robert},
      Booktitle                = {2013 Conference on Lasers \& Electro-Optics Europe \& International Quantum Electronics Conference CLEO EUROPE/IQEC},
      Year                     = {2013},
      Month                    = may,
      Pages                    = {1--1},
      Publisher                = {IEEE},
      Doi                      = {10.1109/CLEOE-IQEC.2013.6801076},
      ISBN                     = {978-1-4799-0594-2},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=6801076}
    }
  • Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon: peckle variance optical coherence tomography for imaging microcirculation. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX Link
    @inproceedings{Klawitter2013,
       author = {Klawitter, Dominico and Hillmann, Dierck and Pieper, M and Steven, Philipp and Wenzel, J and Hüttmann, Gereon},
       title = {Speckle variance optical coherence tomography for
    imaging microcirculation},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    url = { http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.716.940&rep=rep1&type=pdf},
    year = { 2013}
    }
    
  • Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon: Off-axis full-field swept-source optical coherence tomography using holographic refocusing. pp. 857104-857104, 2013
    BibTeX Link Link
    @article{Hillmann2013,
       author = {Hillmann, Dierck and Franke, Gesa and Hinkel, Laura and Bonin, Tim and Koch, Peter and Hüttmann, Gereon},
       title = {Off-axis full-field swept-source optical coherence tomography using holographic refocusing},
       pages = {857104-857104},
       note = {10.1117/12.2006436},
       abstract = {We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.},
       DOI = {10.1117/12.2006436},
       url = {http://dx.doi.org/10.1117/12.2006436},
       year = {2013},
       type = {Journal Article}
    }
    
  • Horstmann, Jens and Brinkmann, Ralf: Non-contact photoacoustic tomography using holographic full field detection. no. 8800, pp. 880007-880007-6, Proc. SPIE, 2013
    BibTeX Link
    @inproceedings{Horstmann2013,
       author = {Horstmann, Jens and Brinkmann, Ralf},
       title = {Non-contact photoacoustic tomography using holographic full field detection},
       publisher = {Proc. SPIE},
       volume = {8800},
       pages = {880007-880007-6},
       note = {10.1117/12.2033599},
       abstract = {An innovative very fast non-contact imaging technique for Photoacoustic Tomography is introduced. It is based on holographic optical speckle detection of a transiently altering surface topography for the reconstruction of absorbing targets. The surface movement is obtained by parallel recording of speckle phase changes known as Electronic Speckle Pattern Interferometry. Due to parallelized 2-D camera detection and repetitive excitation with variable delay with respect to the image acquisition, data recording of whole volumes for Photoacoustic Imaging can be completed in times far below one second. The size of the detected area is scalable by optical magnification. As a proof of concept, an interferometric setup is realized, capable of surface displacement detection with an axial resolution of less than 3 nm. The potential of the proposed method for in vivo Photoacoustic Imaging is discussed.},
       url = {http://dx.doi.org/10.1117/12.2033599},
       type = {Conference Proceedings},
    year = { 2013}
    }
    
  • Klein, Thomas and Wieser, Wolfgang and Reznicek, Lukas and Neubauer, Aljoscha and Kampik, Anselm and Huber, Robert: Multi-MHz retinal OCT. Biomedical optics express, no. 4, pp. 1890-908, n,, 2013
    BibTeX Link Link
    @Article{HU_2013_Klein_a,
      Title                    = {{Multi-MHz retinal OCT}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Reznicek, Lukas and Neubauer, Aljoscha and Kampik, Anselm and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2013},
    
      Month                    = jan,
      Number                   = {10},
      Pages                    = {1890--908},
      Volume                   = {4},
    
      Doi                      = {10.1364/BOE.4.001890},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=boe-4-10-1890}
    }
  • Evers, Michael and Welford, David and Manstein, Dieter and Birngruber, Reginald: Mid-infrared laser spectroscopy using a tunable gain-switched Cr2+: ZnSe laser. in Studierendentagung, Unversität zu Lübeck, 2013
    BibTeX Link
    @inproceedings{Evers2013,
       author = {Evers, Michael and Welford, David and Manstein, Dieter and Birngruber, Reginald},
       title = {Mid-infrared laser spectroscopy using a tunable gain-switched Cr2+: ZnSe laser},
       booktitle = {Studierendentagung},
       publisher = {Unversität zu Lübeck},
       type = {Conference Proceedings},
    URL = { http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.727.5163&rep=rep1&type=pdf},
    year = { 2013}
    }
    
  • Klein, Thomas and Andre, Raphael and Wieser, Wolfgang and Pfeiffer, Tom and Huber, Robert: Joint aperture detection for speckle reduction and increased collection efficiency in ophthalmic MHz OCT. Biomedical optics express, no. 4, pp. 619-34, r,, 2013
    BibTeX Link Link
    @Article{HU_2013_Klein_b,
      Title                    = {{Joint aperture detection for speckle reduction and increased collection efficiency in ophthalmic MHz OCT}},
      Author                   = {Klein, Thomas and Andre, Raphael and Wieser, Wolfgang and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2013},
    
      Month                    = apr,
      Number                   = {4},
      Pages                    = {619--34},
      Volume                   = {4},
    
      Doi                      = {10.1364/BOE.4.000619},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-4-4-619}
    }
  • Theisen-Kunde, D; Wolken, H; Ellebrecht, D; Danicke, V; Wurster, L; Kleemann, M and Birngruber, R: Investigation of water spray to reduce collateral thermal damage during laser resection of soft tissue. no. 8803, pp. 88030F-88030F-4, 2013
    BibTeX Link
    @inproceedings{Theisen-Kunde2013-1,
       author = {Theisen-Kunde, D; Wolken, H; Ellebrecht, D; Danicke, V; Wurster, L; Kleemann, M and Birngruber, R},
       title = {Investigation of water spray to reduce collateral thermal damage during laser resection of soft tissue},
       volume = {8803},
       pages = {88030F-88030F-4},
       note = {10.1117/12.2032384},
       abstract = {To reduce unwanted collateral thermal damage to surrounding tissue and organs during laparoscopic laser dissection (cw, wavelength: 1.9μm) of porcine liver water spray was used. Size and amount of the produced water droplets of the water spray were photographed by short time imaging and analyzed by imaging software. At in vivo measurements on fresh porcine liver the depth of thermal damage was reduced by 85 % with water spray and the lateral size of thermal damage at the tissue surface could be reduced by 67%. This results show that especially for laparoscopic laser surgery water spray application might be a useful tool to avoid unwanted collateral thermal damage.},
       url = {http://dx.doi.org/10.1117/12.2032384},
       type = {Conference Proceedings},
    year = { 2013}
    }
    
  • Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs: Intravascular optical coherence tomography imaging at 3200 frames per second. Optics letters, no. 38, pp. 1715-7, y,, 2013
    BibTeX Link Link
    @Article{HU_2013_Wang_a,
      Title                    = {{Intravascular optical coherence tomography imaging at 3200 frames per second}},
      Author                   = {Wang, Tianshi and Wieser, Wolfgang and Springeling, Geert and Beurskens, Robert and Lancee, Charles T and Pfeiffer, Tom and van der Steen, Antonius F W and Huber, Robert and van Soest, Gijs},
      Journal                  = {Optics letters},
      Year                     = {2013},
    
      Month                    = may,
      Number                   = {10},
      Pages                    = {1715--7},
      Volume                   = {38},
    
      Doi                      = {10.1364/OL.38.001715},
      ISSN                     = {1539-4794},
    keywords = {AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-38-10-1715}
    }
  • Lee, Hee Yoon and Sudkamp, Helge and Marvdashti, Tahereh and Ellerbee, Audrey K.: Interleaved optical coherence tomography. Optics Express, no. 21, pp. 26542-26556, 2013
    BibTeX Link
    @article{Lee2013,
       author = {Lee, Hee Yoon and Sudkamp, Helge and Marvdashti, Tahereh and Ellerbee, Audrey K.},
       title = {Interleaved optical coherence tomography},
       journal = {Optics Express},
       volume = {21},
       number = {22},
       pages = {26542-26556},
       abstract = {We present a novel and cost-effective technique &#x2013; interleaved optical coherence tomography (iOCT) &#x2013; to enhance the imaging speed of swept source OCT systems by acquiring data from multiple lateral positions simultaneously during a single wavelength sweep, using a single detector and a virtually imaged phase array (VIPA) as a multi-band demultiplexer. This technique uses spectral encoding to convert coherence length into higher imaging speed; the speed enhancement factor is independent of the source speed or center wavelength, and the effective A-scan rate scales linearly with sweep speed. The optical configuration requires only a change in the sample arm of a traditional OCT system and preserves the axial resolution and fall-off characteristic of a traditional SS-OCT using the same light source. Using 10kHz, 20kHz and 100kHz sources we provide a first demonstration of image speed enhancement factors of up to 12, 6 and 10, respectively, which yield effective A-scan rates of 120kHz, 120kHz and 1MHz for B-scan imaging, with a sensitivity of up to 82.5 dB. We also show that iOCT can image faster dynamics than traditional OCT B-scan imaging and is capable of 3D biological imaging. The iOCT concept suggests a new route to high-speed OCT imaging for laser developers: that is, by focusing on improving the coherence length and linewidth of existing and emerging sources. Hence, iOCT is a nice complement to ongoing research and commercial efforts to enable faster imaging through development of lasers with faster sweep rates, and offers new hope for existing sources with slow sweep rates and potential for enhancement of coherence length to compete with faster sources to achieve high-speed OCT.},
       keywords = {Optical design of instruments
    Imaging systems
    Coherence imaging
    Optical coherence tomography
    Optical diagnostics for medicine},
       DOI = {10.1364/OE.21.026542},
       url = {http://www.opticsexpress.org/abstract.cfm?URI=oe-21-22-26542},
       year = {2013},
       type = {Journal Article}
    }
    
  • Zhang, Yaokun and Pfeiffer, Tom and Wieser, Wolfgang and Weller, Marcel and Huber, Robert and Klenzner, Thomas and Raczkowsky, Jörg and Wörn, Heinz: History compounding: a novel speckle reduction technique for OCT guided cochleostomy. in SPIE BiOS, pp. 85713H.1-3, International Society for Optics and Photonics, r,, 2013
    BibTeX Link Link
    @InProceedings{HU_2013_Zhang_a,
      Title                    = {{History compounding: a novel speckle reduction technique for OCT guided cochleostomy}},
      Author                   = {Zhang, Yaokun and Pfeiffer, Tom and Wieser, Wolfgang and Weller, Marcel and Huber, Robert and Klenzner, Thomas and Raczkowsky, J\"{o}rg and W\"{o}rn, Heinz},
      Booktitle                = {SPIE BiOS},
      Year                     = {2013},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = mar,
      Pages                    = {85713H.1--3},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.2006979},
    keywords = {AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2006979}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: New High Resolution SRS with ultra broadband spectral coverage. in 2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference, pp. PD_A_8 1-3, Optical Society of America, Munich, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_a,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    address = {Munich},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {2013 Conference on Lasers and Electro-Optics - International Quantum Electronics Conference},
    pages = {PD_A_8 1--3},
    publisher = {Optical Society of America},
    doi={10.1109/CLEOE-IQEC.2013.6801995},
    title = {{FDML Raman: New High Resolution SRS with ultra broadband spectral coverage}},
    url = {https://doi.org/10.1109/CLEOE-IQEC.2013.6801995},
    keywords = {AG-Huber_NL},
    year = { 2013}
    }
  • Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert: FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers. in CLEO: 2013, pp. ATu3P.4 1-3, OSA, 2013
    BibTeX Link Link
    @inproceedings{HU_2013_Karpf_b,
    abstract = {An all fiber based system for high speed, high resolution Raman sensing is presented. The system is based on a wavelength swept Fourier Domain Mode Locked (FDML) laser for the detection of the Raman signal.},
    author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
    booktitle = {CLEO: 2013},
    doi = {10.1364/CLEO_SI.2013.CTu2H.5},
    isbn = {978-1-55752-972-5},
    keywords = {Lasers,Raman,Scattering,Spectroscopy,fiber,stimulated Raman,AG-Huber_NL},
    pages = {ATu3P.4 1--3},
    publisher = {OSA},
    title = {{FDML Raman: High Speed, High Resolution Stimulated Raman Spectroscopy with Rapidly Wavelength Swept Lasers}},
    url = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_SI-2013-CTu2H.5},
    year = { 2013}
    }
  • Bliedtner, Kathrin and Seifert, Eric and Brinkmann, Ralf: emperature induced tissue deformation monitored by dynamic speckle interferometry. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX Link
    @inproceedings{Bliedtner2013,
       author = {Bliedtner, Kathrin and Seifert, Eric and Brinkmann, Ralf},
       title = {Temperature induced tissue deformation monitored
    by dynamic speckle interferometry},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    year = { 2013}
    url = { http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.714.8862&rep=rep1&type=pdf}
    }
    
  • Wurster, Lara M and Warger, W C and Gora, M J and Carruth, R and Tearny, G J and Birngruber, Reginald: Design and development of a miniaturized scanning probe. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX Link
    @inproceedings{Wurster2013,
       author = {Wurster, Lara M and Warger, W C and Gora, M J and Carruth, R and Tearny, G J and Birngruber, Reginald},
       title = {Design and development of a miniaturized scanning probe},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    URL = {http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.726.9913&rep=rep1&type=pdf},
    year = { 2013}
    }
    
  • Cordes, Jens and Nguyen, Felix and Lange, Birgit and Brinkmann, Ralf and Jocham, Dieter: Damage of Stone Baskets by Endourologic Lithotripters: A Laboratory Study of 5 Lithotripters and 4 Basket Types. Advances in Urology, no. 2013, pp. 6, 2013
    BibTeX Link
    @article{Cordes2013,
       author = {Cordes, Jens and Nguyen, Felix and Lange, Birgit and Brinkmann, Ralf and Jocham, Dieter},
       title = {Damage of Stone Baskets by Endourologic Lithotripters: A Laboratory Study of 5 Lithotripters and 4 Basket Types},
       journal = {Advances in Urology},
       volume = {2013},
       pages = {6},
       DOI = {10.1155/2013/632790},
       url = {http://dx.doi.org/10.1155/2013/632790},
       year = {2013},
       type = {Journal Article}
    }
    
  • Koinzer, Stefan and Saeger, Mark and Hesse, Carola and Portz, Lea and Kleemann, Susanne and Schlott, Kerstin and Brinkmann, Ralf and Roider, Johann: Correlation with OCT and histology of photocoagulation lesions in patients and rabbits. Acta Ophthalmologica, pp. no-no, 2013
    BibTeX Link
    @article{Koinzer2013,
       author = {Koinzer, Stefan and Saeger, Mark and Hesse, Carola and Portz, Lea and Kleemann, Susanne and Schlott, Kerstin and Brinkmann, Ralf and Roider, Johann},
       title = {Correlation with OCT and histology of photocoagulation lesions in patients and rabbits},
       journal = {Acta Ophthalmologica},
       pages = {no-no},
       abstract = {Purpose:  To examine spectral domain optical coherence tomographic (OCT) and histological images from comparable retinal photocoagulation lesions in rabbits, and to correlate these images with comparable OCT images from patients. Methods:  508 rabbit lesions were examined by HE-stained paraffin histology. 1019 rabbit lesions versus 236 patient lesions were examined by OCT, all at the time-points 1 hr, 1 week and 4 weeks after photocoagulation. We analysed 100 μm lesions (in humans) and 133 μm lesions (in rabbits) of 200 ms exposures at powers titrated from the histological threshold up to intense damage. Lesions were matched according to morphological criteria. Results:  Dome-shaped layer alterations, retinal infiltration by round, pigmented cells, outer nuclear layer interruption, and eventually full thickness retinal coagulation are detectable in histology and OCT. Horizontal damage extensions are found 1½ times larger in OCT. More intense irradiation was necessary to induce comparable layer affection in rabbit OCT as in histology. Restoration of the inner retinal layers is only shown in the OCT images. Comparable primary lesions caused more pronounced OCT changes in patients than in rabbits during healing. Conclusions:  Optical coherence tomographic images indicate different tissue changes than histologic images. After photocoagulation, they show wider horizontal damage diameters, but underestimate axial damage particularly during healing. Conclusions on retinal restoration should not be drawn from OCT findings alone. Retinal recovery after comparable initial lesions appears to be more complete in rabbit than in patient OCTs.},
       keywords = {histology
    laser
    optical coherence tomography
    photocoagulation
    retina
    retinal healing},
       year = {2013}
    }
    
  • Choi, WooJhon and Mohler, Kathrin J and Potsaid, Benjamin and Lu, Chen D and Liu, Jonathan J and Jayaraman, Vijaysekhar and Cable, Alex E and Duker, Jay S and Huber, Robert and Fujimoto, James G: Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography. PloS one, no. 8, pp. e81499 1-8, n,, 2013
    BibTeX Link
    @Article{HU_2013_Choi_a,
      Title                    = {{Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography}},
      Author                   = {Choi, WooJhon and Mohler, Kathrin J and Potsaid, Benjamin and Lu, Chen D and Liu, Jonathan J and Jayaraman, Vijaysekhar and Cable, Alex E and Duker, Jay S and Huber, Robert and Fujimoto, James G},
      Journal                  = {PloS one},
      Year                     = {2013},
      Month                    = jan,
      Number                   = {12},
      Pages                    = {e81499 1--8},
      Volume                   = {8},
    keywords = {AG-Huber_OCT},
      Doi                      = {10.1371/journal.pone.0081499}
    }
  • Rohde, Ingo and Masch, Jennifer- M. and Theisen-Kunde, Dirk and Marczynski-Bühlow, Martin and Lutter, Georg and Brinkmann, Ralf: Cardiovascular damage after cw and Q-switched 2μm laser irradiation. no. 8803, pp. 88030I-88030I-6, 2013
    BibTeX Link
    @inproceedings{Rohde2013,
       author = {Rohde, Ingo and Masch, Jennifer- M. and Theisen-Kunde, Dirk and Marczynski-Bühlow, Martin and Lutter, Georg and Brinkmann, Ralf},
       title = {Cardiovascular damage after cw and Q-switched 2μm laser irradiation},
       volume = {8803},
       pages = {88030I-88030I-6},
       note = {10.1117/12.2033550},
       abstract = {Aiming for laser-assisted resection of calcified aortic valve structures for Transcatheter Aortic Valve Implantation (TAVI), a Q-switched Tm:YAG laser emitting at a wavelength of 2.01 μm was used to evaluate the cutting efficiency on highly calcified human aortic leaflets in-vitro. The calcified aortic leaflets were examined regarding ablation rates and debris generation, using a pulse energy of 4.3 mJ, a pulse duration of 0.8-1 μs and a repetition rate of 1 kHz. The radiation was transmitted via a 200 μm core diameter quartz fiber. Resection was performed in a fiber-tissue contact mode on water-covered samples in a dish. The remnant particles were analyzed with respect to quantity and size by light microscopy. Additionally, soft tissue of porcine aortic vessels was examined for histologically detectable thermo-mechanical damage after continuous wave and Q-switched 2μm laser irradiation. An ablation rate of 36.7 ± 25.3 mg/min could be realised on highly calcified aortic leaflets, with 85.4% of the remnant particles being &lt;6 μm in diameter. The maximum damaged area of the soft tissue was &lt; 1 mm for both, cw and pulsed laser irradiation. This limits the expected collateral damage of healthy tissue during the medical procedure. Overall, the Q-switched Tm:YAG laser system showed promising results in cutting calcified aortic valves, transmitting sufficient energy through a small flexible fibre.},
       url = {http://dx.doi.org/10.1117/12.2033550},
       type = {Conference Proceedings},
    year = { 2013}
    }
    
  • Seifert, Eric and Roh, Young-Jung and Fritz, Andreas and Park, Young Gun and Kang, Seungbum and Theisen-Kunde, Dirk and Brinkmann, Ralf: Automatic irradiation control by an optical feedback technique for selective retina treatment (SRT) in a rabbit model. no. 8803, pp. 880303-880303-6, 2013
    BibTeX Link
    @inproceedings{Seifert2013,
       author = {Seifert, Eric and Roh, Young-Jung and Fritz, Andreas and Park, Young Gun and Kang, Seungbum and Theisen-Kunde, Dirk and Brinkmann, Ralf},
       title = {Automatic irradiation control by an optical feedback technique for selective retina treatment (SRT) in a rabbit model},
       volume = {8803},
       pages = {880303-880303-6},
    year = {2013},
       note = {10.1117/12.2033560},
       abstract = {Selective Retina Therapy (SRT) targets the Retinal Pigment Epithelium (RPE) without effecting neighboring layers as the photoreceptors or the choroid. SRT related RPE defects are ophthalmoscopically invisible. Owing to this invisibility and the variation of the threshold radiant exposure for RPE damage the treating physician does not know whether the treatment was successful or not. Thus measurement techniques enabling a correct dosing are a demanded element in SRT devices. The acquired signal can be used for monitoring or automatic irradiation control. Existing monitoring techniques are based on the detection of micro-bubbles. These bubbles are the origin of RPE cell damage for pulse durations in the ns and μs time regime 5μs. The detection can be performed by optical or acoustical approaches. Monitoring based on an acoustical approach has already been used to study the beneficial effects of SRT on diabetic macula edema and central serous retinopathy. We have developed a first real time feedback technique able to detect micro-bubble induced characteristics in the backscattered laser light fast enough to cease the laser irradiation within a burst. Therefore the laser energy within a burst of at most 30 pulses is increased linearly with every pulse. The laser irradiation is ceased as soon as micro-bubbles are detected. With this automatic approach it was possible to observe invisible lesions, an intact photoreceptor layer and a reconstruction of the RPE within one week.},
       url = {http://dx.doi.org/10.1117/12.2033560},
       type = {Conference Proceedings}
    }
    
  • Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K: An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array. in Studierendentagung, Universität zu Lübeck, 2013
    BibTeX
    @inproceedings{Sudkamp2013,
       author = {Sudkamp, Helge and Lee, H Y and Hüttmann, Gereon and Kellerbee, A K},
       title = {An approach to increase the speed of Optical Coherence Tomography using a Virtually Imaged Phased Array},
       booktitle = {Studierendentagung},
       publisher = {Universität zu Lübeck},
       type = {Conference Proceedings},
    year= { 2013}
    }
    
  • Baade, Alexander and Schlott, Kerstin and Birngruber, Reginald and Brinkmann, Ralf: A numerical model for heat and pressure propagation for temperature controlled retinal photocoagulation. no. 8803, pp. 88030O-88030O-9, 2013
    BibTeX Link
    @inproceedings{Baade2013,
       author = {Baade, Alexander and Schlott, Kerstin and Birngruber, Reginald and Brinkmann, Ralf},
       title = {A numerical model for heat and pressure propagation for temperature controlled retinal photocoagulation},
       volume = {8803},
       pages = {88030O-88030O-9},
       note = {10.1117/12.2033590},
       abstract = {Retinal photocoagulation is an established treatment for various retinal diseases. The temperature development during a treatment can be monitored by applying short laser pulses in addition to the treatment laser light. The laser pulses induce thermoelastic pressure waves that can be detected at the cornea. We present a numerical model to examine the temperature development during the treatment as well as the formation and propagation of the ultrasonic waves. Using the model, it is possible to determine the peak temperature during retinal photocoagulation from the measured signal, and investigate the behaviour of the temperature profile and the accuracy of the temperature determination under varying conditions such as inhomogeneous pigmentation or change in irradiation parameters. It was shown that there is an uncertainty of 2.5 -9% in the determination of the peak temperature when the absorption coefficient between the absorbing layers is varied by a factor of 2. Furthermore the model was extended in order to incorporate the photoacoustic pressure generation and wave propagation. It was shown that for an irradiation pulse duration of 75 ns the resulting pressure wave energy is attenuated by 76 % due to frequency dependent attenuation in water.},
       url = {http://dx.doi.org/10.1117/12.2033590},
       type = {Conference Proceedings}, 
    year = { 2013}
    }
    
  • Trost, Andrea and Schroedl, Falk and Strohmaier, Clemens A and Bogner, Barbara and Runge, Christian and Kaser-Eichberger, Alexandra and Krefft, Karolina Anna and Vogel, Alfred and Linz, Norbert and Freidank, Sebastian and Hilpert, Andrea and Zimmermann, Inge and Grabner, Günther and Reitsamer, Herbert A: A new nanosecond UV-laser at 355 nm: early results of corneal flap cutting in a rabbit model. Investigative Ophthalmology & Visual Science, 2013
    BibTeX Link
    @article{Trost2013,
       author = {Trost, Andrea and Schroedl, Falk and Strohmaier, Clemens A and Bogner, Barbara and Runge, Christian and Kaser-Eichberger, Alexandra and Krefft, Karolina Anna and Vogel, Alfred and Linz, Norbert and Freidank, Sebastian and Hilpert, Andrea and Zimmermann, Inge and Grabner, Günther and Reitsamer, Herbert A},
       title = {A new nanosecond UV-laser at 355 nm: early results of corneal flap cutting in a rabbit model},
       journal = {Investigative Ophthalmology & Visual Science},
       abstract = {Purpose: A new 355nm UV laser was used for corneal flap cutting in an animal model and tested for clinical and morphological alterations. Methods: Corneal flaps were created (Chinchilla Bastards; n=25) with an UV-nanosecond laser at 355nm (150kHz, pulse duration 850ps, spot-size 1µm, spot-spacing 6x6µm, side-cut Δz 1µm; cutting depth 130µm) and pulse energies of 2.2 or 2.5µJ, respectively. Following slit lamp examination, animals were sacrificed at 6, 12 and 24hrs after treatment. Corneas were prepared for histology (HE, TUNEL-assay) and evaluated statistically, followed by ultrastructural investigations. Results: Laser treatment was tolerated well, flap lift was easier at 2.5µJ compared to 2.2µJ. Standard HE at 24hrs revealed intact epithelium in the horizontal cut, with similar increase in corneal thickness at both energies. Irrespective of energy levels, TUNEL assay revealed comparable numbers of apoptotic cells in the horizontal and vertical cut at 6/12/24hrs, becoming detectable in the horizontal cut as an acellular stromal band at 24hrs. Ultrastructural analysis revealed regular morphology in the epi- and endothelium, while in the stroma, disorganized collagen lamellae were detectable representing the horizontal cut, again irrespective of energy levels applied. Conclusions:This new UV-laser revealed no epithelial nor endothelial damage at energies feasible for corneal flap cutting. Observed corneal swelling was lower compared to existing UV-lasers-studies, albeit total energy applied here was much higher. Observed loss of stromal keratinocytes is comparable to available laser systems. Therefore, this new laser is suitable for refractive surgery, awaiting its test in a chronic environment.},
       DOI = {10.1167/iovs.13-12580},
       url = {http://www.iovs.org/content/early/2013/10/28/iovs.13-12580.abstract},
       year = {2013},
       type = {Journal Article}
    }
    

2012

  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX
    @article{Miura2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    eng
    England
    2012/03/06 06:00
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       year = {2012}
    }
    
  • Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.: Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process. Exp Eye Res, no. 97, pp. 63-72, 2012
    BibTeX Link
    @article{Treumer2012,
       author = {Treumer, F. and Klettner, A. and Baltz, J. and Hussain, A. A. and Miura, Y. and Brinkmann, R. and Roider, J. and Hillenkamp, J.},
       title = {Vectorial release of matrix metalloproteinases (MMPs) from porcine RPE-choroid explants following selective retina therapy (SRT): towards slowing the macular ageing process},
       journal = {Exp Eye Res},
       volume = {97},
       number = {1},
       pages = {63-72},
       note = {1096-0007
    Treumer, F
    Klettner, A
    Baltz, J
    Hussain, A A
    Miura, Y
    Brinkmann, R
    Roider, J
    Hillenkamp, J
    Journal Article
    England
    Exp Eye Res. 2012 Apr;97(1):63-72. doi: 10.1016/j.exer.2012.02.011. Epub 2012 Feb 22.},
       abstract = {The purpose of this study was to investigate release of matrix metalloproteinases (MMP) 2 and 9 during retinal pigment epithelium (RPE) wound healing after Selective Retina Therapy (SRT) with laser energy levels below and above the threshold of RPE cell death. Following exposure to SRT using a prototype pulsed Nd:YLF laser with energies of 80-180 mJ/cm(2) fresh porcine RPE-monolayers with Bruch's membrane and choroid were cultured in modified Ussing chambers which separate the apical (RPE-facing) and basal (choroid facing) sides of the RPE monolayer. Threshold energy for RPE cell death and wound healing were determined with calcein-AM viability test. Inactive and active forms of MMP 2 and 9 were quantified within tissue samples and in the culture medium of the apical and basal compartments of the Ussing chamber using gelatine zymography. Laser energies of 160-180 mJ/cm(2) resulted in cell death within 1 h while 120-140 mJ/cm(2) resulted in delayed death of exposed RPE cells. All cells survived 80 and 100 mJ/cm(2). Laser spots healed within 6 days after SRT accompanied by a transient vectorial increase of MMPs. SRT with 180 mJ/cm(2) increased active MMP 2 by 1.9 (p < 0.05) and 1.6 (p < 0.05) fold in tissue and basal compartments, respectively, without alterations in the apical compartment. Pro-MMP 2 levels were also significantly increased in all compartments (p < 0.05). Release of MMP 9 was not altered. Laser energy below the threshold of RPE cell death did not alter the release of MMP 2 or 9. The findings suggest that the release of active MMP 2 on the basal side of the RPE during wound healing following SRT may address age-related pathological changes of Bruch's membrane with a potential to slow degenerative macular ageing processes before irreversible functional loss has occurred.},
       keywords = {Animals
    Cell Death
    Cell Survival
    Choroid/*enzymology/pathology
    Diffusion Chambers, Culture
    Fluoresceins/metabolism
    *Laser Therapy
    Lasers, Solid-State
    Macular Degeneration/enzymology/pathology/*surgery
    Matrix Metalloproteinase 2/*metabolism
    Matrix Metalloproteinase 9/*metabolism
    Organ Culture Techniques
    Retinal Pigment Epithelium/*enzymology/pathology
    Sensory Thresholds
    Swine
    Wound Healing/*physiology},
       ISSN = {0014-4835},
       DOI = {10.1016/j.exer.2012.02.011},
       year = {2012},
       type = {Journal Article}
    }
    
  • Unglert, C. I. and Warger, W. C. and Hostens, J. and Namati, E. and Birngruber, R. and Bouma, B. E. and Tearney, G. J.: Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography. Journal of Biomedical Optics, no. 17, 2012
    BibTeX Link
    @article{Unglert2012,
       author = {Unglert, C. I. and Warger, W. C. and Hostens, J. and Namati, E. and Birngruber, R. and Bouma, B. E. and Tearney, G. J.},
       title = {Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {12},
       note = {083ZB
    Times Cited:3
    Cited References Count:23},
       abstract = {Optical coherence tomography (OCT) has been increasingly used for imaging pulmonary alveoli. Only a few studies, however, have quantified individual alveolar areas, and the validity of alveolar volumes represented within OCT images has not been shown. To validate quantitative measurements of alveoli from OCT images, we compared the cross-sectional area, perimeter, volume, and surface area of matched subpleural alveoli from microcomputed tomography (micro-CT) and OCT images of fixed air-filled swine samples. The relative change in size between different alveoli was extremely well correlated (r > 0.9, P < 0.0001), but OCT images underestimated absolute sizes compared to micro-CT by 27% (area), 7% (perimeter), 46% (volume), and 25% (surface area) on average. We hypothesized that the differences resulted from refraction at the tissue-air interfaces and developed a ray-tracing model that approximates the reconstructed alveolar size within OCT images. Using this model and OCT measurements of the refractive index for lung tissue (1.41 for fresh, 1.53 for fixed), we derived equations to obtain absolute size measurements of superellipse and circular alveoli with the use of predictive correction factors. These methods and results should enable the quantification of alveolar sizes from OCT images in vivo. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.12.126015]},
       keywords = {alveolar structure
    alveolar volume
    ray-tracing
    optical coherence tomography
    refractive-index
    tissue},
       ISSN = {1083-3668},
       DOI = {Artn 126015
    Doi 10.1117/1.Jbo.17.12.126015},
       url = {<Go to ISI>://WOS:000314504400023},
       year = {2012},
       type = {Journal Article}
    }
    
  • Blatter, Cedric and Klein, Thomas and Grajciar, Branislav and Schmoll, Tilman and Wieser, Wolfgang and Andre, Raphael and Huber, Robert and Leitgeb, Rainer A: Ultrahigh-speed non-invasive widefield angiography. Journal of biomedical optics, no. 17, pp. 070505 1-3, l,, 2012
    BibTeX Link Link
    @Article{HU_2012_Blatter_c,
      Title                    = {{Ultrahigh-speed non-invasive widefield angiography}},
      Author                   = {Blatter, Cedric and Klein, Thomas and Grajciar, Branislav and Schmoll, Tilman and Wieser, Wolfgang and Andre, Raphael and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Journal of biomedical optics},
      Year                     = {2012},
    
      Month                    = jul,
      Number                   = {7},
      Pages                    = {070505 1--3 },
      Volume                   = {17},
    
      Doi                      = {10.1117/1.JBO.17.7.070505},
      ISSN                     = {1560-2281},
    keywords = {AG-Huber_OCT},
      Url                      = {http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1351611}
    }
  • Gehlsen, U. and Oetke, A. and Szaszak, M. and Koop, N. and Paulsen, F. and Gebert, A. and Huettmann, G. and Steven, P.: Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro. Graefes Arch Clin Exp Ophthalmol, no. 6, pp. 6, 2012
    BibTeX
    @article{Gehlsen,
       author = {Gehlsen, U. and Oetke, A. and Szaszak, M. and Koop, N. and Paulsen, F. and Gebert, A. and Huettmann, G. and Steven, P.},
       title = {Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro},
       journal = {Graefes Arch Clin Exp Ophthalmol},
       volume = {6},
       pages = {6},
       note = {Using Smart Source Parsing
    May},
       abstract = {BACKGROUND: Early and correct diagnosis of delayed or absent corneal epithelial wound healing is a key factor in the prevention of infection and consecutive destruction of the corneal stroma with impending irreversible visual loss. Two-photon microscopy (TPM) is a novel technology that has potential to depict epithelial cells and to evaluate cellular function by measuring autofluorescence properties such as fluorescence intensity and fluorescence lifetimes of metabolic co-factors such as NAD(P)H. METHODS: Using non-invasive TPM in a tissue-culture scratch model and an organ-culture erosion model, fluorescence intensity and fluorescence lifetimes of NAD(P)H were measured before and during closure of the epithelial wounds. Influence of temperature and selective inhibition of metabolism on intensity and lifetimes were tested additionally. RESULTS: Decrease of temperature resulted in significant increase of fluorescence lifetimes and decrease of the relative amount of free NAD(P)H due to decreased global metabolism. Increase in temperature and upregulation of glycolysis through blocking the mitochondrial electron transport chain by rotenone resulted in increased intensity, decreased lifetimes and increase in the relative amount of free NAD(P)H. Changes of lifetimes and free:protein-bound NAD(P)H ratios were similar to changes measured during wound healing in both scratch and erosion models. CONCLUSIONS: Fluorescence lifetime measurements (FLIM) detected enhancement of cellular metabolism following epithelial damage in both models. The prospective detection of cellular autofluorescence in vivo, in particular FLIM of metabolic cofactor NAD(P)H, has the potential to become an indispensible tool in clinical use to differentiate healing from non-healing epithelial cells and to evaluate effects of newly developed substances on cellular metabolism in preclinical and clinical trials.},
       year = {2012}
    }
  • Rohde, Ingo and Brinkmann, Ralf and Theisen-Kunde, Dirk: Temporally stretched Q-switched pulses in the 2 µm spectral range. Laser Physics Letters, no. 9, pp. 808-813, 2012
    BibTeX
    @article{Rohde2012,
       author = {Rohde, Ingo and Brinkmann, Ralf and Theisen-Kunde, Dirk},
       title = {Temporally stretched Q-switched pulses in the 2 µm spectral range},
       journal = {Laser Physics Letters},
       volume = {9},
       number = {11},
       pages = {808-813},
       year = {2012}
    }
    
  • Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.: Temperature-controlled retinal photocoagulation - a step toward automated laser treatment. Invest Ophthalmol Vis Sci, no. 53, pp. 3605-14, 2012
    BibTeX
    @article{Koinzer2012,
       author = {Koinzer, S. and Schlott, K. and Ptaszynski, L. and Bever, M. and Kleemann, S. and Saeger, M. and Baade, A. and Caliebe, A. and Miura, Y. and Birngruber, R. and Brinkmann, R. and Roider, J.},
       title = {Temperature-controlled retinal photocoagulation - a step toward automated laser treatment},
       journal = {Invest Ophthalmol Vis Sci},
       volume = {53},
       number = {7},
       pages = {3605-14},
       note = {Using Smart Source Parsing
    Jun 14; Print 2012 Jul},
       abstract = {Purpose. Retinal laser photocoagulation carries the risk of overtreatment due to effect variation of identically applied lesions. The degree of coagulation depends on the induced temperature increase and on exposure time. We introduce temperature controlled photocoagulation (TCP), which uses optoacoustics to determine individually exposure times necessary to create reproducible lesions. Methods. Optoacoustic temperature measurement relies on pressure waves that are excited in the retinal tissue by repetitive low-energy laser pulses. Signal amplitudes correlate with tissue temperature and are detected by a transducer in the laser contact lens. We used a continuous wave (CW) photocoagulator for treatment irradiation and superimposed probe laser pulses for simultaneous temperature measurement. Optoacoustic data of 1500 lesions (rabbit) were evaluated to develop an algorithm that controls exposure times automatically in TCP. Lesion diameters of 156 TCP lesions were compared to 156 non-controlled lesions. Histology was performed after 1 hour, and 1 and 4 weeks. Results. TCP resulted in exposure times from 4 to 800 ms depending on laser power chosen. Ophthalmoscopic and histologic lesion diameters were independent of power between 14 and 200 mW. TCP lesions barely were visible with a mean diameter equal to the treatment beam (130 mum). In contrast, standard lesion diameters increased linearly and statistically significantly with power. Histology confirmed sparing of the ganglion and nerve fiber layers in TCP. Conclusions. TCP facilitates uniform retinal lesions over a wide power range. In a clinical setting, it should generate soft and reproducible lesions independently of local tissue variation and improve safety, particularly at short exposure times.},
       year = {2012}
    }
  • Iwami, Hisashi and Ptaszynski, Lars and Danicke, Veit and Brinkmann, Ralf and Miura, Yoko: Sublethal Hyperthermia-induced Vascular Endothelial Growth Factor Secretion And Its Contribution To Adoptive Response Of Retinal Pigment Epithelial Cell. Invest. Ophthalmol. Vis. Sci., no. 53, pp. 4782-, 2012
    BibTeX Link
    @article{Iwami2012,
       author = {Iwami, Hisashi and Ptaszynski, Lars and Danicke, Veit and Brinkmann, Ralf and Miura, Yoko},
       title = {Sublethal Hyperthermia-induced Vascular Endothelial Growth Factor Secretion And Its Contribution To Adoptive Response Of Retinal Pigment Epithelial Cell},
       journal = {Invest. Ophthalmol. Vis. Sci.},
       volume = {53},
       number = {6},
       pages = {4782-},
       abstract = {PurposeTo investigate temperature increase-induced secretion of vascular endothelial growth factor (VEGF) from retinal pigment epithelial (RPE) cells and its contribution to adoptive response relating to cell defence system against oxidative stress. MethodsPorcine RPE cells on 35 mm culture dish were used in the study. Thulium laser ({lambda}=1940 nm, spot size 33 mm was utilized as a heat source. Temperature increase during irradiation for different power and time setting at cell level was measured with thermocouple, and power and time setting of the experiment was determined based on this calibration. Culture medium was replaced by 1.2 ml phosphate buffer saline and then laser was irradiated with different power settings for 10 seconds, so that the peak temperature reaches from 40{degrees}C to 65{degrees}C. Cellular viability after laser irradiation was examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay immediately after irradiation. VEGF secretion was investigated with enzyme-linked immunosorbent assay (ELISA) at 2 and 24 hrs after irradiation. Contribution of a temperature-dependent calcium channel, TRPV (transient receptor potential vanilloid) channels in laser-induced VEGF secretion was investigated using TRPV channel blocker, ruthenium red (20 {micro}M). TRPV channel blocker-containing medium was replaced by the normal medium soon after laser irradiation. Hydrogen peroxide (H2O2) or advanced glycation endproduct (AGE)-was exposed after 6 hrs of laser irradiation and cell viability was examined with MTT assay. ResultsPeak temperature threshold for immediate RPE cell death was found around 55 {degrees}C with our irradiation setting. VEGF secretion was increased after sub-lethal irradiation in power-dependent manner, which was partially suppressed by TRPV channel blocker. Sublethal laser irradiation reduced H2O2 and AGE-induced cell death and this effect was smaller in the cells treated with TRPV channel inhibitor during laser irradiation. ConclusionsSublethal temperature increase-induced VEGF production might contribute to the enhancement of RPE cell defence system against oxidative stress.},
       url = {http://abstracts.iovs.org/cgi/content/abstract/53/6/4782},
       year = {2012},
       type = {Journal Article}
    }
    
  • Blatter, Cedric and Grajciar, Branislav and Hermann, Boris and Huber, Robert and Drexler, Wolfgang and Leitgeb, Rainer A: Simultaneous dark-bright field swept source OCT for ultrasound detection. in SPIE BiOS, pp. 82131M 1-3, International Society for Optics and Photonics, b,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Blatter_d,
      Title                    = {{Simultaneous dark-bright field swept source OCT for ultrasound detection}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Hermann, Boris and Huber, Robert and Drexler, Wolfgang and Leitgeb, Rainer A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82131M 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.911443},
    keywords = {AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.911443}
    }
  • Brinkmann, Ralf and Koinzer, Stefan and Schlott, Kerstin and Ptaszynski, Lars and Bever, Marco and Baade, Alexander and Luft, Susanne and Miura, Yoko and Roider, Johann and Birngruber, Reginald: Real-time temperature determination during retinal photocoagulation on patients. Journal of Biomedical Optics, no. 17, pp. 061219, 2012
    BibTeX
    @article{Brinkmann2012,
       author = {Brinkmann, Ralf and Koinzer, Stefan and Schlott, Kerstin and Ptaszynski, Lars and Bever, Marco and Baade, Alexander and Luft, Susanne and Miura, Yoko and Roider, Johann and Birngruber, Reginald},
       title = {Real-time temperature determination during retinal photocoagulation on patients},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {6},
       pages = {061219},
       note = {Journal Article},
       year = { 2012}
    }
  • Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim: Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy. no. 8213, pp. 821338, SPIE, 2012
    BibTeX Link
    @inproceedings{Ansari2012,
       author = {Ansari, Rehman and Aherrahrou, Redouane and Aherrahrou, Zouhair and Erdmann, Jeanette and Huttmann, Gereon and Schweikard, Achim},
       title = {Quantitative analysis of cardiomyocyte dynamics with optical coherence phase microscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821338},
    year = { 2012},
    url = {https://doi.org/10.1117/12.911781}
    
    }
  • Klein, Thomas and Wieser, Wolfgang and Huber, Robert: OPTICAL COHERENCE TOMOGRAPHY/HIGH-SPEED BIOMEDICAL IMAGING: No speed limit: The multi-megahertz approach to optical coherence tomography. BioOptics World, no. 5, pp. 28-32, 2012
    BibTeX Link Link
    @Article{HU_2012_Klein_a,
      Title                    = {{OPTICAL COHERENCE TOMOGRAPHY/HIGH-SPEED BIOMEDICAL IMAGING: No speed limit: The multi-megahertz approach to optical coherence tomography}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Huber, Robert},
      Journal                  = {BioOptics World},
      Year                     = {2012},
      Number                   = {1},
      Pages                    = {28--32},
      Volume                   = {5},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Doi                      = {http://www.bioopticsworld.com/articles/print/volume-5/issue-1/features/the-multi-megahertz-approach-to-optical-coherence-tomography.html},
      Url                      = {http://www.bioopticsworld.com/articles/print/volume-5/issue-1/features/the-multi-megahertz-approach-to-optical-coherence-tomography.html}
    }
  • Klein, Thomas and Wieser, Wolfgang and Andre, Raphael and Pfeiffer, Tom and Eigenwillig, Christoph M and Huber, Robert: Multi-MHz FDML OCT: snapshot retinal imaging at 6.7 million axial-scans per second. in SPIE BiOS, pp. 82131E 1-3, International Society for Optics and Photonics, b,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Klein_b,
      Title                    = {{Multi-MHz FDML OCT: snapshot retinal imaging at 6.7 million axial-scans per second}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Andre, Raphael and Pfeiffer, Tom and Eigenwillig, Christoph M and Huber, Robert},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82131E 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.908798},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.908798}
    }
  • Blatter, Cedric and Grajciar, Branislav and Zou, Pu and Wieser, Wolfgang and Verhoef, Aart-Jan and Huber, Robert and Leitgeb, Rainer A: Intrasweep phase-sensitive optical coherence tomography for noncontact optical photoacoustic imaging. Optics letters, no. 37, pp. 4368-70, OSA, v,, 2012
    BibTeX Link Link
    @Article{HU_2012_Blatter_b,
      Title                    = {{Intrasweep phase-sensitive optical coherence tomography for noncontact optical photoacoustic imaging}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Zou, Pu and Wieser, Wolfgang and Verhoef, Aart-Jan and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Optics letters},
      Year                     = {2012},
    
      Month                    = nov,
      Number                   = {21},
      Pages                    = {4368--70},
      Volume                   = {37},
    
      Doi                      = {10.1364/OL.37.004368},
      ISSN                     = {1539-4794},
      Pmid                     = {23114298},
      Publisher                = {OSA},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://ol.osa.org/abstract.cfm?URI=ol-37-21-4368 http://www.ncbi.nlm.nih.gov/pubmed/23114298}
    }
  • Blatter, Cedric and Weingast, Jessika and Alex, Aneesh and Grajciar, Branislav and Wieser, Wolfgang and Drexler, Wolfgang and Huber, Robert and Leitgeb, Rainer A: In situ structural and microangiographic assessment of human skin lesions with high-speed OCT. Biomedical optics express, no. 3, pp. 2636-46, OSA, t,, 2012
    BibTeX Link Link
    @Article{HU_2012_Blatter_a,
      Title                    = {{In situ structural and microangiographic assessment of human skin lesions with high-speed OCT}},
      Author                   = {Blatter, Cedric and Weingast, Jessika and Alex, Aneesh and Grajciar, Branislav and Wieser, Wolfgang and Drexler, Wolfgang and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = oct,
      Number                   = {10},
      Pages                    = {2636--46},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002636},
      ISSN                     = {2156-7085},
      Pmid                     = {23082302},
      Publisher                = {OSA},
    keywords = {AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-10-2636 http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3469999\&tool=pmcentrez\&rendertype=abstract}
    }
  • Mueller, H. H. and Ptaszynski, L. and Schlott, K. and Debbeler, C. and Bever, M. and Koinzer, S. and Birngruber, R. and Brinkmann, R. and Huettmann, G.: Imaging thermal expansion and retinal tissue changes during photocoagulation by high speed OCT. Biomedical Optics Express, no. 3, pp. 1025-1046, 2012
    BibTeX Link
    @article{Müller2012,
       author = {Mueller, H. H. and Ptaszynski, L. and Schlott, K. and Debbeler, C. and Bever, M. and Koinzer, S. and Birngruber, R. and Brinkmann, R. and Huettmann, G.},
       title = {Imaging thermal expansion and retinal tissue changes during photocoagulation by high speed OCT},
       journal = {Biomedical Optics Express},
       volume = {3},
       number = {5},
       pages = {1025-1046},
       note = {935RH
    Times Cited:8
    Cited References Count:37},
       abstract = {Visualizing retinal photocoagulation by real-time OCT measurements may considerably improve the understanding of thermally induced tissue changes and might enable a better reproducibility of the ocular laser treatment. High speed Doppler OCT with 860 frames per second imaged tissue changes in the fundus of enucleated porcine eyes during laser irradiation. Tissue motion, measured by Doppler OCT with nanometer resolution, was correlated with the temperature increase, which was measured non-invasively by optoacoustics. In enucleated eyes, the increase of the OCT signal near the retinal pigment epithelium (RPE) corresponded well to the macroscopically visible whitening of the tissue. At low irradiance, Doppler OCT revealed additionally a reversible thermal expansion of the retina. At higher irradiance additional movement due to irreversible tissue changes was observed. Measurements of the tissue expansion were also possible in vivo in a rabbit with submicrometer resolution when global tissue motion was compensated. Doppler OCT may be used for spatially resolved measurements of retinal temperature increases and thermally induced tissue changes. It can play an important role in understanding the mechanisms of photocoagulation and, eventually, lead to new strategies for retinal laser treatments. (c) 2012 Optical Society of America},
       keywords = {optical coherence tomography
    laser photocoagulation
    vein occlusion
    management
    diseases
    fundus
    blood},
       ISSN = {2156-7085},
       url = {<Go to ISI>://WOS:000303537400018},
       year = {2012},
       type = {Journal Article}
    }
    
  • Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon: Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography. no. 8213, pp. 82131S, SPIE, 2012
    BibTeX Link
    @inproceedings{Spahr2012,
       author = {Spahr, Hendrik and Rudolph, Linda and Muller, Heike and Birngruber, Reginald and Huttmann, Gereon},
       title = {Imaging of photothermal tissue expansion via phase sensitive optical coherence tomography},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131S},
    year = { 2012},
    url = { https://doi.org/10.1117/12.911429}
    
    }
  • Hüttmann, G. and Orzekowsky-Schröder, R.: Imaging of immune cell dynamics in small intestine and the eye by 2-photon microcopy. in 7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012), 2012
    BibTeX
    @inproceedings{Hüttmann2012,
       author = {Hüttmann, G. and Orzekowsky-Schröder, R.},
       title = {Imaging of immune cell dynamics in small intestine and the eye  by 2-photon microcopy},
       booktitle = {7th Workshop on Advanced Multiphoton and Fluorescence Lifetime Imaging Techniques (FLIM 2012)},
    year = { 2012}
       abstract = {Autofluorescence based 2-photon microscopy was investigated as a
    tool for studying the dynamics of immunological processes in vivo. Nearly
    all tissue components were simultaneously visible by autofluorescence and a
    nearly complete visualization of tissue architecture was possible. Within
    the tissue, immune competent cells like lymphocytes, macrophages and
    dendritic cells were visualized in their dynamic interaction with other
    cells or surrounding tissues.
    Immunological processes were studied in the small intestine and at the
    surface of the eye. Excitation and emission spectra of the different mucosal
    tissue components were quantitatively determined and a compared to the
    respective spectra of endogenous chromophores. It was shown, that by using
    only two excitation wavelengths within the tuning range of a Ti:Saphire
    laser enterocytes, antigen presenting cells and lysosomes of the small
    intestine could be discriminated based on the excitation and emission
    properties. By additionally using an intravital nuclear stain, motion of
    lymphocytes in the lamina propria and the epithelium of small intestine
    villi was quantitatively analyzed. 
    
    2-photon microscopy was also a powerful tool for studying the conjunctiva
    and cornea of the eye. Lymphocyte dynamics and uptake of microspheres or
    fluorescing heat-inactivated E. coli was followed over time in the
    conjunctiva-associated lymphoid tissue of mice. In a different mouse model
    of suture induced corneal hem- and lymphangiogenesis, immune cell migration
    into lymphatic vessels and luminal transport of individual cells was
    observed in vivo.
    
    Autofluorescence based 2-photon intravital microscopy is a valuable tool to
    study dynamic immunological processes. While tissue autofluorescence gives a
    good overview over all relevant structures and allows for discriminating
    different cell types by spectral analysis, the additional combination of
    specific staining with external dyes or fluorescent proteins is easily
    possible and enhances the potential of the technique further. },
    
    }
  • Marschall, Sebastian and Torzicky, Teresa and Klein, Thomas and Wieser, Wolfgang and Pircher, Michael and Götzinger, Erich and Zotter, Stefan and Bonesi, Marco and Biedermann, Benjamin and Pedersen, Christian and Huber, Robert and Hitzenberger, Christoph and Andersen, Peter: High-speed polarization-sensitive OCT at 1060 nm using a Fourier domain mode-locked swept source. in SPIE Photonics Europe, pp. 84271D-84271D-6, International Society for Optics and Photonics, n,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Marschall_a,
      Title                    = {{High-speed polarization-sensitive OCT at 1060 nm using a Fourier domain mode-locked swept source}},
      Author                   = {Marschall, Sebastian and Torzicky, Teresa and Klein, Thomas and Wieser, Wolfgang and Pircher, Michael and G\"{o}tzinger, Erich and Zotter, Stefan and Bonesi, Marco and Biedermann, Benjamin and Pedersen, Christian and Huber, Robert and Hitzenberger, Christoph and Andersen, Peter},
      Booktitle                = {SPIE Photonics Europe},
      Year                     = {2012},
      Editor                   = {Popp, J\"{u}rgen and Drexler, Wolfgang and Tuchin, Valery V and Matthews, Dennis L},
      Month                    = jun,
      Pages                    = {84271D--84271D--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.922313},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1219832}
    }
  • Bonesi, Marco and Sattmann, Harald and Torzicky, Teresa and Zotter, Stefan and Baumann, Bernhard and Pircher, Michael and Götzinger, Erich and Eigenwillig, Christoph and Wieser, Wolfgang and Huber, Robert and Hitzenberger, Christoph K: High-speed polarization sensitive optical coherence tomography scan engine based on Fourier domain mode locked laser. Biomedical optics express, no. 3, pp. 2987-3000, v,, 2012
    BibTeX Link Link
    @Article{HU_2012_Bonesi_a,
      Title                    = {{High-speed polarization sensitive optical coherence tomography scan engine based on Fourier domain mode locked laser}},
      Author                   = {Bonesi, Marco and Sattmann, Harald and Torzicky, Teresa and Zotter, Stefan and Baumann, Bernhard and Pircher, Michael and G\"{o}tzinger, Erich and Eigenwillig, Christoph and Wieser, Wolfgang and Huber, Robert and Hitzenberger, Christoph K},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = nov,
      Number                   = {11},
      Pages                    = {2987--3000},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002987},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-11-2987}
    }
  • Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon: High resolution holoscopy. no. 8213, pp. 821324, SPIE, 2012
    BibTeX Link
    @inproceedings{Franke 2012,
       author = {Franke, Gesa Lilith and Hillmann, Dierck and Claussen, Thorsten and Luhrs, Christian and Koch, Peter and Huttmann, Gereon},
       title = {High resolution holoscopy},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {821324},
    URL = { https://doi.org/10.1117/12.911166},
    year = { 2012}
    
    }
  • Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander: Feasibiliy of optical detection of soft tissue deformation during needle insertion. no. 8316, pp. 83160V, SPIE, 2012
    BibTeX Link
    @inproceedings{Otte2012,
       author = {Otte, Christoph and Huttmann, Gereon and Schlaefer, Alexander},
       title = {Feasibiliy of optical detection of soft tissue deformation during needle insertion},
       editor = {David, R. Holmes, III and Kenneth, H. Wong},
       publisher = {SPIE},
       volume = {8316},
       pages = {83160V},
    year = { 2012},
    url = { https://www.spiedigitallibrary.org/conference-proceedings-of-spie/8316/83160V/Feasibiliy-of-optical-detection-of-soft-tissue-deformation-during-needle/10.1117/12.912538.short}
    
    }
  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert: Extended coherence length megahertz FDML and its application for anterior segment imaging. Biomedical optics express, no. 3, pp. 2647-57, t,, 2012
    BibTeX Link Link
    @Article{HU_2012_Wieser_b,
      Title                    = {{Extended coherence length megahertz FDML and its application for anterior segment imaging}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Eigenwillig, Christoph M and Karpf, Sebastian and Schmitt, Joseph M and Huber, Robert},
      Journal                  = {Biomedical optics express},
      Year                     = {2012},
    
      Month                    = oct,
      Number                   = {10},
      Pages                    = {2647--57},
      Volume                   = {3},
    
      Doi                      = {10.1364/BOE.3.002647},
      ISSN                     = {2156-7085},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-10-2647}
    }
    
  • Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon: Efficient holoscopy image reconstruction. Opt. Express, no. 20, pp. 21247-21263, 2012
    BibTeX
    @article{Hillmann2012,
       author = {Hillmann, Dierck and Franke, Gesa and Lührs, Christian and Koch, Peter and Hüttmann, Gereon},
       title = {Efficient holoscopy image reconstruction},
       journal = {Opt. Express},
       volume = {20},
       number = {19},
       pages = {21247-21263},
       abstract = {Holoscopy is a tomographic imaging technique that combines digital holography and Fourier-domain optical coherence tomography (OCT) to gain tomograms with diffraction limited resolution and uniform sensitivity over several Rayleigh lengths. The lateral image information is calculated from the spatial interference pattern formed by light scattered from the sample and a reference beam. The depth information is obtained from the spectral dependence of the recorded digital holograms. Numerous digital holograms are acquired at different wavelengths and then reconstructed for a common plane in the sample. Afterwards standard Fourier-domain OCT signal processing achieves depth discrimination. Here we describe and demonstrate an optimized data reconstruction algorithm for holoscopy which is related to the inverse scattering reconstruction of wavelength-scanned full-field optical coherence tomography data. Instead of calculating a regularized pseudoinverse of the forward operator, the recorded optical fields are propagated back into the sample volume. In one processing step the high frequency components of the scattering potential are reconstructed on a non-equidistant grid in three-dimensional spatial frequency space. A Fourier transform yields an OCT equivalent image of the object structure. In contrast to the original holoscopy reconstruction with backpropagation and Fourier transform with respect to the wavenumber, the required processing time does neither depend on the confocal parameter nor on the depth of the volume. For an imaging NA of 0.14, the processing time was decreased by a factor of 15, at higher NA the gain in reconstruction speed may reach two orders of magnitude.},
       keywords = {Image processing
    Optical coherence tomography
    Digital holography},
       year = {2012}
    }
    
  • Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert: Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment. in Conference on Lasers and Electro-Optics 2012, pp. JTh3J.2 1-3, OSA, Washington, D.C., 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Wieser_c,
      Title                    = {{Dispersion Compensated Megahertz FDML Laser for Imaging of the Anterior Segment}},
      Author                   = {Wieser, Wolfgang and Klein, Thomas and Adler, Desmond C and Trepanier, Francois and Karpf, Sebastian and Eigenwillig, Christoph and Schmitt, Joseph M and Huber, Robert},
      Booktitle                = {Conference on Lasers and Electro-Optics 2012},
      Year                     = {2012},
    
      Address                  = {Washington, D.C.},
      Pages                    = {JTh3J.2 1--3},
      Publisher                = {OSA},
    
      Doi                      = {10.1364/CLEO_AT.2012.JTh3J.2},
      ISBN                     = {978-1-55752-943-5},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_AT-2012-JTh3J.2}
    }
  • Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer: Deep skin structural and microcirculation imaging with extended-focus OCT. in SPIE BiOS, pp. 82070B-82070B-7, International Society for Optics and Photonics, b,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Blatter_e,
      Title                    = {{Deep skin structural and microcirculation imaging with extended-focus OCT}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Month                    = feb,
      Pages                    = {82070B--82070B--7},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.909830},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1310560}
    }
  • Koinzer, Stefan and Schlott, Kerstin and Portz, Lea and Ptaszynski, Lars and Baade, Alexander and Bever, Marco and Saeger, Mark and Caliebe, Amke and Denner, Renè and Birngruber, Reginald and Brinkmann, Ralf and Roider, Johann: Correlation of temperature rise and optical coherence tomography characteristics in patient retinal photocoagulation. Journal of Biophotonics, pp. n/a-n/a, 2012
    BibTeX
    @article{Koinzer,
       author = {Koinzer, Stefan and Schlott, Kerstin and Portz, Lea and Ptaszynski, Lars and Baade, Alexander and Bever, Marco and Saeger, Mark and Caliebe, Amke and Denner, Renè and Birngruber, Reginald and Brinkmann, Ralf and Roider, Johann},
       title = {Correlation of temperature rise and optical coherence tomography characteristics in patient retinal photocoagulation},
       journal = {Journal of Biophotonics},
       pages = {n/a-n/a},
       abstract = {We conducted a study to correlate the retinal temperature rise during photocoagulation to the afterward detected tissue effect in optical coherence tomography (OCT). 504 photocoagulation lesions were examined in 20 patients. The retinal temperature increase was determined in real-time during treatment based on thermoelastic tissue expansion which was probed by repetitively applied ns laser pulses. The tissue effect was examined on fundus images and OCT images of individualized lesions. We discerned seven characteristic morphological OCT lesion classes. Their validity was confirmed by increasing visibility and diameters. Mean peak temperatures at the end of irradiation ranged from approx. 60 °C to beyond 100 °C, depending on burn intensity. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)},
       keywords = {laser photocoagulation
    optoacoustics
    photocoagulation
    retinal temperature
    spectral domain optical coherence tomography
    OCT
    subthreshold
    classification},
       year = {2012}
    }
  • Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.: Confocal endomicroscopy of the larynx. no. 8207, pp. 82072H, SPIE, 2012
    BibTeX
    @inproceedings{Just 2012,
       author = {Just, T. and Wiechmann, T. and Stachs, O. and Stave, J. and Guthoff, R. and Huttmann, G. and Pau, H. W.},
       title = {Confocal endomicroscopy of the larynx},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian Jet-Fei, Wong and Justus, F. Ilgner and Krzysztof, Izdebski and Melissa, J. Suter and Stephen, Lam and Matthew, Brenner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, Madsen and Anita, Mahadevan-Jansen and Jansen, E. Duco and Andreas, Mandelis},
       publisher = {SPIE},
       volume = {8207},
       pages = {82072H},
    year = { 2012}
    
    }
  • Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.: Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy. Histochem Cell Biol, no. 137, pp. 269-278, 2012
    BibTeX Link
    @article{Klinder2012,
       author = {Klinger, A. and Orzekowsky-Schroeder, R. and von Smolinski, D. and Blessenohl, M. and Schueth, A. and Koop, N. and Hüttmann, G. and Gebert, A.},
       title = {Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy},
       journal = {Histochem Cell Biol},
       volume = {137},
       number = {3},
       pages = {269-278},
       ISSN = {1432-119X (Electronic)
    0948-6143 (Linking)},
       DOI = {10.1007/s00418-011-0905-0},
       year = {2012},
       type = {Journal Article}
    }
    
  • Hillmann, D. and Bonin, T. and Luhrs, C. and Franke, G. and Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Common approach for compensation of axial motion artifacts in swept-source OCT and dispersion in Fourier-domain OCT. Opt Express, no. 20, pp. 6761-6776, Mar, 2012
    BibTeX
    @article{Hillmann2012,
       author={Hillmann, D.  and Bonin, T.  and Luhrs, C.  and Franke, G.  and Hagen-Eggert, M.  and Koch, P.  and Huttmann, G. },
       title={{{C}ommon approach for compensation of axial motion artifacts in swept-source {O}{C}{T} and dispersion in {F}ourier-domain {O}{C}{T}}},
       journal={Opt Express},
       year={2012},
       volume={20},
       number={6},
       pages={6761--6776},
       month={Mar}
    }
    
  • Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A: Coherence length extension of Fourier domain mode locked lasers. in SPIE BiOS, pp. 82130O 1-3, International Society for Optics and Photonics, b,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Adler_a,
      Title                    = {{Coherence length extension of Fourier domain mode locked lasers}},
      Author                   = {Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82130O 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.908148},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.908148}
    }
  • Wieser, Wolfgang and Palte, Gesa and Eigenwillig, Christoph M and Biedermann, Benjamin R and Pfeiffer, Tom and Huber, Robert: Chromatic polarization effects of swept waveforms in FDML lasers and fiber spools. Optics express, no. 20, pp. 9819-32, r,, 2012
    BibTeX Link Link
    @Article{HU_2012_Wieser_a,
      Title                    = {{Chromatic polarization effects of swept waveforms in FDML lasers and fiber spools}},
      Author                   = {Wieser, Wolfgang and Palte, Gesa and Eigenwillig, Christoph M and Biedermann, Benjamin R and Pfeiffer, Tom and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2012},
    
      Month                    = apr,
      Number                   = {9},
      Pages                    = {9819--32},
      Volume                   = {20},
    
      Doi                      = {10.1364/OE.20.009819},
      ISSN                     = {1094-4087},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-9-9819\&id=231991}
    }
  • Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Torzicky, Teresa and Pircher, Michael and Biedermann, Benjamin R and Pedersen, Christian and Hitzenberger, Christoph K and Huber, Robert and Andersen, Peter E: Broadband Fourier domain mode-locked laser for optical coherence tomography at 1060 nm. in SPIE BiOS, pp. 82130R 1-3, International Society for Optics and Photonics, b,, 2012
    BibTeX Link Link
    @InProceedings{HU_2012_Marschall_b,
      Title                    = {{Broadband Fourier domain mode-locked laser for optical coherence tomography at 1060 nm}},
      Author                   = {Marschall, Sebastian and Klein, Thomas and Wieser, Wolfgang and Torzicky, Teresa and Pircher, Michael and Biedermann, Benjamin R and Pedersen, Christian and Hitzenberger, Christoph K and Huber, Robert and Andersen, Peter E},
      Booktitle                = {SPIE BiOS},
      Year                     = {2012},
      Editor                   = {Izatt, Joseph A and Fujimoto, James G and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {82130R 1--3 },
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.906148},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.906148}
    }
  • Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon: Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction. Journal of Biomedical Optics, no. 17, pp. 058003, 2012
    BibTeX
    @article{Rudnitzki2012,
       author = {Rudnitzki, Florian and Bever, Marco and Rahmanzadeh, Ramtin and Brieger, Katrin and Endl, Elmar and Groll, Jurgen and Huttmann, Gereon},
       title = {Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {5},
       pages = {058003},
       year = {2012}
    }
  • Todor, Sebastian and Biedermann, Benjamin and Huber, Robert and Jirauschek, Christian: Balance of physical effects causing stationary operation of Fourier domain mode-locked lasers. Journal of the Optical Society of America B, no. 29, pp. 656-664, OSA, 2012
    BibTeX Link Link
    @Article{HU_2012_Todor_a,
      Title                    = {{Balance of physical effects causing stationary operation of Fourier domain mode-locked lasers}},
      Author                   = {Todor, Sebastian and Biedermann, Benjamin and Huber, Robert and Jirauschek, Christian},
      Journal                  = {Journal of the Optical Society of America B},
      Year                     = {2012},
      Number                   = {4},
      Pages                    = {656--664 },
      Volume                   = {29},
    
      Doi                      = {10.1364/JOSAB.29.000656},
      Publisher                = {OSA},
    keywords = {AG-Huber_FDML},
      Url                      = {http://josab.osa.org/abstract.cfm?URI=josab-29-4-656}
    }
  • Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Bever, Marco and Baade, Alex and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf: Automatic temperature controlled retinal photocoagulation. Journal of Biomedical Optics, no. 17, pp. 061223, 2012
    BibTeX Link
    @article{Schlott2012,
       author = {Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Bever, Marco and Baade, Alex and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Automatic temperature controlled retinal photocoagulation},
       journal = {Journal of Biomedical Optics},
       volume = {17},
       number = {6},
       pages = {061223},
       keywords = {AutoPhoN},
       year = {2012}
    }
    
  • Hagen-Eggert, M. and Koch, P. and Huttmann, G.: Analysis of the signal fall-off in spectral domain optical coherence tomography systems. no. 8213, pp. 82131K, SPIE, 2012
    BibTeX Link
    @inproceedings{Hagen-Eggert,
       author = {Hagen-Eggert, M. and Koch, P. and Huttmann, G.},
       title = {Analysis of the signal fall-off in spectral domain optical coherence tomography systems},
       editor = {Joseph, A. Izatt and James, G. Fujimoto and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {8213},
       pages = {82131K},
    url = {https://doi.org/10.1117/12.910921},
       year = { 2012}
    
    }
    

2011

  • Eigenwillig, Christoph M and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert: Wavelength swept amplified spontaneous emission source for high speed retinal optical coherence tomography at 1060 nm. Journal of biophotonics, no. 4, pp. 552-8, g,, 2011
    BibTeX Link Link
    @Article{HU_2011_Eigenwillig_a,
      Title                    = {{Wavelength swept amplified spontaneous emission source for high speed retinal optical coherence tomography at 1060 nm}},
      Author                   = {Eigenwillig, Christoph M and Klein, Thomas and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Journal of biophotonics},
      Year                     = {2011},
    
      Month                    = aug,
      Number                   = {7-8},
      Pages                    = {552--8},
      Volume                   = {4},
    
      Doi                      = {10.1002/jbio.201000104},
      ISSN                     = {1864-0648},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://onlinelibrary.wiley.com/doi/10.1002/jbio.201000104/abstract}
    }
  • Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon: Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina. no. 7889, pp. 788906, SPIE, 2011
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Hagen-Eggert, Martin and Franke, Gesa and Koch, Peter and Huttmann, Gereon},
       title = {Ultra highspeed in-vivo Fourier domain full-field OCT of the human retina},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {788906},
    year = { 2011}
    
    }
  • Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.: The proliferation marker Ki-67 as novel molecular target for PDT. Photodiagnosis and Photodynamic Therapy, no. 8, pp. 129, 2011
    BibTeX Link
    @article{Rahmanzadeh2011,
       author = {Rahmanzadeh, R. and Celli, J. and Rizvi, I. and Gerdes, J. and Hasan, T.},
       title = {The proliferation marker Ki-67 as novel molecular target for PDT},
       journal = {Photodiagnosis and Photodynamic Therapy},
       volume = {8},
       number = {2},
     pages = {129},
       DOI = {10.1016/j.pdpdt.2011.03.025},
       year = {2011},
       type = {Journal Article}
    }
    
  • Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba: Targeted molecular effects through laser-irradiated nanoabsorbers. 2011
    BibTeX Link
    @inproceedings{Hüttmann2011,
       author = {Hüttmann, Gereon and Rahmanzadeh, Ramtin and Rudnitzki, Florian and Endl, Elmar and Hasan, Tayyaba},
       title = {Targeted molecular effects through laser-irradiated nanoabsorbers},
       editor = {Newsrrom, SPIE},
       DOI = {10.1117/2.1201102.003548},
       type = {Conference Proceedings},
       year = { 2011}
    }
    
  • Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer A: Structural and functional imaging with extended focus dark-field OCT at 1300nm. in SPIE BiOS, pp. 78891D-78891D-6, International Society for Optics and Photonics, b,, 2011
    BibTeX Link Link
    @InProceedings{HU_2011_Blatter_c,
      Title                    = {{Structural and functional imaging with extended focus dark-field OCT at 1300nm}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Huber, Robert and Leitgeb, Rainer A},
      Booktitle                = {SPIE BiOS},
      Year                     = {2011},
      Editor                   = {Fujimoto, James G and Izatt, Joseph A and Tuchin, Valery V},
      Month                    = feb,
      Pages                    = {78891D--78891D--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.875594},
    keywords = {AG-Huber_OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=732654}
    }
  • Klatt, C. and Saeger, M. and Oppermann, T. and Porksen, E. and Treumer, F. and Hillenkamp, J. and Fritzer, E. and Brinkmann, R. and Birngruber, R. and Roider, J.: Selective retina therapy for acute central serous chorioretinopathy. Br J Ophthalmol, no. 95, pp. 83-8, 2011
    BibTeX
    @article{Klatt,
       author = {Klatt, C. and Saeger, M. and Oppermann, T. and Porksen, E. and Treumer, F. and Hillenkamp, J. and Fritzer, E. and Brinkmann, R. and Birngruber, R. and Roider, J.},
       title = {Selective retina therapy for acute central serous chorioretinopathy},
       journal = {Br J Ophthalmol},
       volume = {95},
       number = {1},
       pages = {83-8},
       note = {Using Smart Source Parsing
    Jan; Epub 2010 Jun 15},
       abstract = {AIMS: To evaluate selective retina therapy (SRT) as a treatment of acute central serous chorioretinopathy. METHODS: 30 eyes of 30 patients with central serous chorioretinopathy of at least a 3 months' duration were recruited. 14 eyes were randomised to an SRT group (Q-switched neodymium-doped yttrium lithium fluoride (Nd:YLF) laser, wavelength 527 nm, t=1.7 mus, energy 100-370 muJ, spot diameter 200 mum, pulse repetition rate 100 Hz,) and 16 eyes to a control group. After 3 months of follow-up, patients in the control group with persistence of subretinal fluid (SRF) were allocated to a cross-over group, treated with SRT and followed up for further 3 months. The main outcome measures were change of best-corrected Early Treatment Diabetic Retinopathy Study visual acuity (BCVA) and SRF. RESULTS: At 3 months of follow-up, the mean (SD) improvement of BCVA was significantly greater after SRT than in the control group: 12.7 (7.2) versus 6.3 (8.9) letters (p=0.04). SRF had decreased significantly more after SRT as compared with that the control group: 203 (136) mum versus 41 (150) mum (p=0.005). In eight eyes allocated to the cross-over group, the mean BCVA had increased during 3 months of follow up before SRT by 1.4 (5.2) letters and continued to increase during 3 months following SRT by 7.4 (6.3) letters, while SRF increased by 39.5 (160.2) mum before SRT and decreased by 151.5 (204.9) mum after SRT. In six of the eight eyes, SRF had completely resolved 3 months after SRT. CONCLUSIONS: SRT appears to expedite functional recovery and the re-absorption of SRF as compared with that in untreated controls. A larger prospective, randomised phase 3 confirmative patient study is warranted. TRIAL REGISTRATION NUMBER: NCT00987077.},
       year = {2011}
    }
  • Miura, Yoko: Retinal pigment epithelium-choroid organ culture. Expert Rev Ophthalmol, no. 6, pp. 669-680, 2011
    BibTeX
    @article{Miura2011,
       author = {Miura, Yoko},
       title = {Retinal pigment epithelium-choroid organ culture},
       journal = {Expert Rev  Ophthalmol},
       volume = {6},
       number = {6},
       pages = {669-680},
       abstract = {The retinal pigment epithelium (RPE) plays a vital role in retinal function, and therefore studies on RPE provide a significant benefit for our visual function. In order to obtain useful information from study results, choosing the suitable experimental model for each purpose of study is of great importance. Although RPE cell cultures are widely used, cells in cell culture have significantly different phenotypical characteristics from in vivo cells. The advantage of using native tissue is that cells in the tissue have close biological properties to in vivo conditions. This review describes basic characteristics of native RPE–choroid tissues in comparison to RPE cells in cell culture and introduces the possibility of preserving tissues in different culture systems. Advantages and disadvantages of organ culture and suitable studies with recent study results are also introduced.},
       year = {2011}
    }
  • Brinkmann, R and Koinzer, S and Schlott, K and Ptaszynski, L and Bever, M and Baade, A and Miura, Y and Birngruber, R and Roider, J: Realtime temperature determination during retinal photocoagulation on patients.. Proc SPIE, no. 7885, pp. 78850R, 2011
    BibTeX
    @article{Brinkmann2011,
       author = {Brinkmann, R and Koinzer, S and Schlott, K and Ptaszynski, L and Bever, M and Baade, A and Miura, Y and Birngruber, R and Roider, J},
       title = {Realtime temperature determination during retinal photocoagulation on patients.},
       journal = {Proc SPIE},
       volume = {7885},
       pages = {78850R},
       abstract = {Retinal photocoagulation is a long time established treatment for a variety of retinal diseases, most commonly applied for diabetic macular edema and diabetic retinopathy. The damage extent of the induced thermal coagulations depend on the temperature increase and the time of irradiation. So far, the induced temperature rise is unknown due to intraocular variations in light transmission and scattering and RPE/choroidal pigmentation, which can vary inter- and intraindividually by more than a factor of four. Thus in clinical practice, often stronger and deeper coagulations are applied than therapeutically needed, which lead to extended retinal damage and strong pain perception. The final goal of this project focuses on a dosimetry control, which automatically generates a desired temperature profile and thus coagulation strength for every individual coagulation spot, ideally unburden the ophthalmologist from any laser settings. In this paper we present the first realtime temperature measurements achieved on patients during retinal photocoagulation by means of an optoacoustic method, making use of the temperature dependence of the thermal expansion coefficient of retinal tissue. Therefore, nanosecond probe laser pulses are repetitively and simultaneously applied with the treatment radiation in order to excite acoustic waves, which are detected at the cornea with an ultrasonic transducer embedded in the contact lens and then are processed by PC.},
       url = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=732381},
       year = {2011},
       type = {Journal Article}
    }
    
  • Vogel, A and Venugopalan, V.: Pulsed laser ablation of tissue. Springer, Heidelberg, New York, 2011
    BibTeX
    @book{Vogel 2011-2,
       author = {Vogel, A and Venugopalan, V.},
       title = {Pulsed laser ablation of tissue},
       publisher = {Springer, Heidelberg, New York},
       edition = {2},
       year = { 2011}
    }
  • Vogel, Alfred and Venugopalan, Vasan: Pulsed Laser Ablation of Soft Biological Tissues. in Optical-Thermal Response of Laser-Irradiated Tissue, pp. 551-615, 2011
    BibTeX
    @inbook{Vogel 2011,
       author = {Vogel, Alfred and Venugopalan, Vasan},
       title = {Pulsed Laser Ablation of Soft Biological Tissues},
       booktitle = {Optical-Thermal Response of Laser-Irradiated Tissue},
       pages = {551-615},
       year = { 2011}
    }
    
  • Horstmann, Jens and Baade, Alexander and Brinkmann, Ralf: Photoacoustic blood vessel detection during surgical laser interventions. no. 8092, pp. 80920Z-80920Z-6, SPIE ECBO, 2011
    BibTeX Link
    @inproceedings{Horstmann2011,
       author = {Horstmann, Jens and Baade, Alexander and Brinkmann, Ralf},
       title = {Photoacoustic blood vessel detection during surgical laser interventions},
       publisher = {SPIE ECBO},
       volume = {8092},
       pages = {80920Z-80920Z-6},
       note = {10.1117/12.889635},
       abstract = {This paper presents a discussion about the potential of photoacoustics with regard to its application in surgical assistance during minimally invasive, laser assisted interventions. Aim of the work is the detection of obscured large blood vessels in order to prevent unintentional dissection. Based on spectroscopic investigations of the target tissue (liver), a wavelength for the photoacoustic excitation laser was chosen with respect to a high absorption contrast between the vessel and the surrounding liver tissue. An experimental setup featuring a simple liver model is created. Preliminary results show, that vessels with a diameter of 2 mm can be detected up to a distance of 1 mm from the treatment fibre. It is shown, that detection of acoustic waves induced inside liver is feasible over distances higher than 10 cm.},
       url = {http://dx.doi.org/10.1117/12.889635},
       type = {Conference Proceedings},
    year = { 2011}
    }
    
  • Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Luft, Susanne and Baade, Alex and Bever, Marco and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf: Optoacoustic temperature determination and automatic coagulation control in rabbits. in Ophthalmic Technologies XXI, no. 7885, Proc. SPIE, 2011
    BibTeX Link
    @inproceedings{Schlott2011,
       author = {Schlott, Kerstin and Koinzer, Stefan and Ptaszynski, Lars and Luft, Susanne and Baade, Alex and Bever, Marco and Roider, Johann and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Optoacoustic temperature determination and automatic coagulation control in rabbits},
       booktitle = {Ophthalmic Technologies XXI },
       editor = {Ho, Fabrice Manns; Per G. Söderberg; Arthur},
       publisher = {Proc. SPIE},
       volume = {7885},
       note = {10.1117/12.875104},
       abstract = {Retinal laser photocoagulation is an established treatment method for many retinal diseases like macula edema or diabetic retinopathy. The selection of the laser parameters is so far based on post treatment evaluation of the lesion size and strength. Due to local pigment variations in the fundus and individual transmission the same laser parameters often lead to an overtreatment. Optoacoustic allows a non invasive monitoring of the retinal temperature increase during retinal laser irradiation by measuring the temperature dependent pressure amplitudes, which are induced by short probe laser pulses. A 75 ns/ 523 nm Nd:YLF was used as a probe laser at a repetition rate of 1 kHz, and a cw / 532 nm treatment laser for heating. A contact lens was modified with a ring-shaped ultrasonic transducer to detect the pressure waves at the cornea. Temperatures were collected for irradiations leading to soft or invisible lesions. Based on this data the threshold for denaturation was found. By analyzing the initial temperature increase, the further temperature development during irradiation could be predicted. An algorithm was found to calculate the irradiation time, which is needed for a soft lesion formation, from the temperature curve. By this it was possible to provide a real-time dosimetry by automatically switching off the treatment laser after the calculated irradiation time. Automatically controlled coagulations appear softer and more uniformly.},
       keywords = {AutoPhoN},
       url = {http://dx.doi.org/10.1117/12.875104},
       type = {Conference Proceedings},
    year = { 2011}
    }
    
  • Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.: OCT in the field of laryngology: further perspectives. no. 7883, pp. 78831W, SPIE, 2011
    BibTeX
    @inproceedings{Just2011,
       author = {Just, T. and Pau, H. W. and Lankenau, E. and Huttmann, G.},
       title = {OCT in the field of laryngology: further perspectives},
       editor = {Nikiforos, Kollias and Bernard, Choi and Haishan, Zeng and Hyun Wook, Kang and Bodo, E. Knudsen and Brian, J. Wong and Justus, F. R. Ilgner and Kenton, W. Gregory and Guillermo, J. Tearney and Laura, Marcu and Henry, Hirschberg and Steen, J. Madsen and Andreas, Mandelis and Anita, Mahadevan-Jansen and Jansen, E. Duco},
       publisher = {SPIE},
       volume = {7883},
       pages = {78831W},
    Year = { 2011}
    
    }
  • Klein, Thomas and Wieser, Wolfgang and Eigenwillig, Christoph M and Biedermann, Benjamin R and Huber, Robert: Megahertz OCT for ultrawide-field retinal imaging with a 1050 nm Fourier domain mode-locked laser. Optics express, no. 19, pp. 3044-62, b,, 2011
    BibTeX Link Link
    @Article{HU_2011_Klein_a,
      Title                    = {{Megahertz OCT for ultrawide-field retinal imaging with a 1050 nm Fourier domain mode-locked laser}},
      Author                   = {Klein, Thomas and Wieser, Wolfgang and Eigenwillig, Christoph M and Biedermann, Benjamin R and Huber, Robert},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = feb,
      Number                   = {4},
      Pages                    = {3044--62},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.003044},
      ISSN                     = {1094-4087},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-4-3044}
    }
  • Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus: Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels. PLoS One, no. 6(10), pp. e26253, 2011
    BibTeX Link
    @article{Steven2011,
       author = {Steven, Philipp and Bock, Felix and Hüttmann, Gereon and Cursiefen, Claus},
       title = {Intravital Two-Photon Microscopy of Immune Cell Dynamics in Corneal Lymphatic Vessels},
       journal = {PLoS One},
       volume = {6(10)},  
    url = { https://doi.org/10.1371/journal.pone.0026253},
       pages = {e26253},
       abstract = {<sec> <title>Background</title> <p>The role of lymphatic vessels in tissue and organ transplantation as well as in tumor growth and metastasis has drawn great attention in recent years.</p> </sec> <sec> <title>Methodology/Principal Findings</title> <p>We now developed a novel method using non-invasive two-photon microscopy to simultaneously visualize and track specifically stained lymphatic vessels and autofluorescent adjacent tissues such as collagen fibrils, blood vessels and immune cells in the mouse model of corneal neovascularization in vivo. The mouse cornea serves as an ideal tissue for this technique due to its easy accessibility and its inducible and modifiable state of pathological hem- and lymphvascularization.</p> <p>Neovascularization was induced by suture placement in corneas of Balb/C mice. Two weeks after treatment, lymphatic vessels were stained intravital by intrastromal injection of a fluorescently labeled LYVE-1 antibody and the corneas were evaluated in vivo by two-photon microscopy (TPM). Intravital TPM was performed at 710 nm and 826 nm excitation wavelengths to detect immunofluorescence and tissue autofluorescence using a custom made animal holder. Corneas were then harvested, fixed and analyzed by histology.</p> <p>Time lapse imaging demonstrated the first in vivo evidence of immune cell migration into lymphatic vessels and luminal transport of individual cells. Cells immigrated within 1–5.5 min into the vessel lumen. Mean velocities of intrastromal corneal immune cells were around 9 µm/min and therefore comparable to those of T-cells and macrophages in other mucosal surfaces.</p> </sec> <sec> <title>Conclusions</title> <p>To our knowledge we here demonstrate for the first time the intravital real-time transmigration of immune cells into lymphatic vessels. Overall this study demonstrates the valuable use of intravital autofluorescence two-photon microscopy in the model of suture-induced corneal vascularizations to study interactions of immune and subsequently tumor cells with lymphatic vessels under close as possible physiological conditions.</p> </sec>},
       year = { 2011}
    }
  • Todor, Sebastian and Biedermann, Benjamin and Wieser, Wolfgang and Huber, Robert and Jirauschek, Christian: Instantaneous lineshape analysis of Fourier domain mode-locked lasers. Optics express, no. 19, pp. 8802-7, r,, 2011
    BibTeX Link Link
    @Article{HU_2011_Todor_a,
      Title                    = {{Instantaneous lineshape analysis of Fourier domain mode-locked lasers}},
      Author                   = {Todor, Sebastian and Biedermann, Benjamin and Wieser, Wolfgang and Huber, Robert and Jirauschek, Christian},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = apr,
      Number                   = {9},
      Pages                    = {8802--7},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.008802},
      Institution              = {Institute for Nanoelectronics, Technische Universit\"{a}t M\"{u}nchen, Munich, Germany. todor@tum.de},
      ISSN                     = {1094-4087},
    keywords = {AG-Huber_FDML},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-9-8802\&seq=0}
    }
  • Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon: In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence. Journal of Biomedical Optics, no. 16, pp. 116025, 2011
    BibTeX
    @article{Orzekowsky2011,
       author = {Orzekowsky-Schroeder, Regina and Klinger, Antje and Martensen, Bjorn and Blessenohl, Maike and Gebert, Andreas and Vogel, Alfred and Huttmann, Gereon},
       title = {In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence},
       journal = {Journal of Biomedical Optics},
       volume = {16},
       number = {11},
       pages = {116025},
       keywords = {biological organs
    biomedical optical imaging
    cellular biophysics
    fluorescence
    laser applications in medicine
    two-photon processes},
       year = {2011}
    }
  • Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon: Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT. no. 7889, pp. 78890E, SPIE, 2011
    BibTeX Link
    @inproceedings{Müller 2011-2,
       author = {Muller, Heike H. and Ptaszynski, Lars and Schlott, Kerstin and Bonin, Tim and Bever, Marco and Koinzer, Stefan and Birngruber, Reginald and Brinkmann, Ralf and Huttmann, Gereon},
       title = {Imaging of temperature distribution and retinal tissue changes during photocoagulation by high speed OCT},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78890E},
    URL = { https://doi.org/10.1117/12.874788},
    year = { 2011}
    
    }
  • Hillmann, Dierck and Luhrs, Christian and Bonin, Tim and Koch, Peter and Vogel, Alfred and Huttmann, Gereon: Holoscopy: holographic optical coherence tomography. no. 8091, pp. 80911H, Proc. SPIE, 2011
    BibTeX Link
    @inproceedings{Hillmann2011,
       author = {Hillmann, Dierck and Luhrs, Christian  and Bonin, Tim  and Koch, Peter and Vogel, Alfred  and Huttmann, Gereon },
       title = {Holoscopy: holographic optical coherence tomography},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {Proc. SPIE},
       volume = {8091},
       pages = {80911H},
       url = {http://link.aip.org/link/?PSI/8091/80911H/1
    http://dx.doi.org/10.1117/12.889485},
       type = {Conference Proceedings},
    year = { 2011}
    }
    
  • Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.: Holoscopy-holographic optical coherence tomography. Opt Lett, no. 36, pp. 2390-2, 2011
    BibTeX Link Link
    @article{Hillmann2011-1,
       author = {Hillmann, D. and Luhrs, C. and Bonin, T. and Koch, P. and Huttmann, G.},
       title = {Holoscopy--holographic optical coherence tomography},
       journal = {Opt Lett},
       volume = {36},
       number = {13},
       pages = {2390-2},
       abstract = {Scanning optical coherence tomography (OCT) is limited in sensitivity and resolution by the restricted focal depth of the confocal detection scheme. Holoscopy, a combination of holography and Fourier-domain full-field OCT, is proposed as a way to detect photons from all depths of a sample volume simultaneously with uniform sensitivity and lateral resolution, even at high NAs. By using the scalar diffraction theory, as frequently applied in digital holographic imaging, we fully reconstruct the object field with depth-invariant imaging quality. In vivo imaging of human skin is demonstrated with an image quality comparable to conventionally scanned OCT.},
       keywords = {Fingers
    Fourier Analysis
    Holography/*methods
    Humans
    Photons
    Tomography, Optical Coherence/*methods},
       ISSN = {1539-4794 (Electronic)
    0146-9592 (Linking)},
       DOI = {10.1364/OL.36.002390},
       url = {http://www.ncbi.nlm.nih.gov/pubmed/21725421},
       year = {2011},
       type = {Journal Article}
    }
    
  • Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A: High-speed functional OCT with self-reconstructive Bessel illumination at 1300 nm. in European Conferences on Biomedical Optics, pp. 809104-809104-6, International Society for Optics and Photonics, n,, 2011
    BibTeX Link Link
    @InProceedings{HU_2011_Blatter_b,
      Title                    = {{High-speed functional OCT with self-reconstructive Bessel illumination at 1300 nm}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A},
      Booktitle                = {European Conferences on Biomedical Optics},
      Year                     = {2011},
      Editor                   = {Leitgeb, Rainer A and Bouma, Brett E},
      Month                    = jun,
      Pages                    = {809104--809104--6},
      Publisher                = {International Society for Optics and Photonics},
    
      Doi                      = {10.1117/12.889669},
    keywords = {AG-Huber_FDML, AG-Huber_ OCT},
      Url                      = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1272395}
    }
  • Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan: Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell. PLoS Pathog, no. 7, pp. e1002108, 2011
    BibTeX
    @article{Szaszak,
       author = {Szaszák, Márta and Steven, Philipp and Shima, Kensuke and Orzekowsky-Schröder, Regina and Hüttmann, Gereon and König, Inke R. and Solbach, Werner and Rupp, Jan},
       title = {Fluorescence Lifetime Imaging Unravels <italic>C. trachomatis</italic> Metabolism and Its Crosstalk with the Host Cell},
       journal = {PLoS Pathog},
       volume = {7},
       number = {7},
       pages = {e1002108},
       abstract = {<title>Author Summary</title> <p>Separate analysis of host and pathogen metabolic changes in intracellular <italic>C. trachomatis</italic> infections is arduous and has not been comprehensively realized so far. A more detailed understanding about the metabolic activity and needs of <italic>C. trachomatis</italic> and its specific interactions with the host cell would be the basis for the development of novel treatment strategies. We therefore applied fluorescence lifetime imaging (FLIM) of the metabolic coenzymes NAD(P)H using two-photon microscopy to directly visualize metabolic changes of host cells and pathogens in living cells. NAD(P)H fluorescence was detected both on the chlamydial inclusion membrane and inside the inclusion. Interestingly, changes in chlamydial growth and progeny induced by glucose starvation and IFN? treatment were directly linked to significant changes of the NAD(P)H fluorescence lifetimes inside the inclusions. Furthermore, measurement of the NAD(P)H fluorescence lifetime in the host cell nucleus revealed that infected cells were programmed for starvation during the metabolically active phase of intracellular chlamydial growth. Our findings highlight for the first time a direct interaction between host and pathogen metabolism in intracellular bacterial infections that exceeds sole competition for nutrients. In conclusion, fluorescence lifetime imaging of NAD(P)H by two-photon microscopy enables real-time analysis of metabolic host-pathogen interactions in intracellular infections with high spatial and temporal resolution.</p>},
       year = {2011}
    }
  • Klein, T and Wieser, W and Biedermann, B R and Eigenwillig, C M and Huber, R: FDML laser for megahertz retinal OCT imaging. in 2011 Conference on Lasers and Electro-Optics (CLEO), EEE, 2011
    BibTeX
    @InProceedings{HU_2011_Klein_b,
      Title                    = {{FDML laser for megahertz retinal OCT imaging}},
      Author                   = {Klein, T and Wieser, W and Biedermann, B R and Eigenwillig, C M and Huber, R},
      Booktitle                = {2011 Conference on Lasers and Electro-Optics (CLEO)},
      Year                     = {2011},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Publisher                = {IEEE}
    }
  • Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A: Extended focus high-speed swept source OCT with self-reconstructive illumination. Optics express, no. 19, pp. 12141-55, n,, 2011
    BibTeX Link Link
    @Article{HU_2011_Blatter_a,
      Title                    = {{Extended focus high-speed swept source OCT with self-reconstructive illumination}},
      Author                   = {Blatter, Cedric and Grajciar, Branislav and Eigenwillig, Christoph M and Wieser, Wolfgang and Biedermann, Benjamin R and Huber, Robert and Leitgeb, Rainer A},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = jun,
      Number                   = {13},
      Pages                    = {12141--55},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.012141},
      ISSN                     = {1094-4087},
    keywords = {AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-19-13-12141}
    }
  • Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A: Extended coherence length Fourier domain mode locked lasers at 1310 nm. Optics express, no. 19, pp. 20930-9, OSA, t,, 2011
    BibTeX Link Link
    @Article{HU_2011_Adler_a,
      Title                    = {{Extended coherence length Fourier domain mode locked lasers at 1310 nm}},
      Author                   = {Adler, Desmond C and Wieser, Wolfgang and Trepanier, Francois and Schmitt, Joseph M and Huber, Robert A},
      Journal                  = {Optics express},
      Year                     = {2011},
    
      Month                    = oct,
      Number                   = {21},
      Pages                    = {20930--9},
      Volume                   = {19},
    
      Doi                      = {10.1364/OE.19.020930},
      ISSN                     = {1094-4087},
      Publisher                = {OSA},
    keywords = {AG-Huber_FDML, AG-Huber_OCT},
      Url                      = {http://www.opticsinfobase.org/abstract.cfm?uri=oe-19-21-20930}
    }
  • Fritz, Andreas and Zegelin, Andrea and Ptaszynski, Lars and Birngruber, Reginald and Brinkmann, Ralf: Dynamics of laser induced micro bubble clusters on tissue phantoms. no. 7885, pp. 78850S-78850S-6, 2011
    BibTeX Link
    @inproceedings{Fritz2011,
       author = {Fritz, Andreas and Zegelin, Andrea and Ptaszynski, Lars and Birngruber, Reginald and Brinkmann, Ralf},
       title = {Dynamics of laser induced micro bubble clusters on tissue phantoms},
       volume = {7885},
       pages = {78850S-78850S-6},
       note = {10.1117/12.875031},
       abstract = {Selective retina treatment (SRT) is a laser based method to treat retinal diseases associated with disorders of the retinal pigment epithelium (RPE) while preserving photoreceptors and choroid. Applying microsecond laser pulses to the 100- 200 strongly absorbing melanin granules inside the RPE cells induces transient micro bubbles which disrupt the cells. Aim of this work is to understand bubble dynamics in clusters with respect to the influence of the adjacent retina. Bubble dynamics were investigated in vitro on porcine RPE. An about 200 μm thick layer of agarose gel was applied to the RPE layer in order to simulate the mechanical properties of retina. Different laser pulse durations from 1 ns (532 nm, Nd:YAG) to 1.7 μs (527 nm, Nd:YLF) were used. The bubbles were investigated interferometrically (fiber interferometer @ 830 nm) and with fast flash photography (25 ns flash duration). Bubble lifetimes were measured. The results show that with retina phantoms the bubble formation threshold was reached at 2.5 times higher irradiation than without retina phantom for 1.7 μs laser pulses. The microbubbles generated with 1 ns laser pulses were almost not influenced by the agarose layer. Irradiation twofold over bubble formation threshold resulted in 3.5 times longer bubble lifetimes for μs and 2 times longer for ns pulse durations, respectively.},
       url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/7885/1/Dynamics-of-laser-induced-micro-bubble-clusters-on-tissue-phantoms/10.1117/12.875031.short},
       type = {Conference Proceedings},
    year = { 2011}
    }
    
  • Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.: Diffusion-sensitive Fourier-domain optical coherence tomography. no. 7889, pp. 78892B, SPIE, 2011
    BibTeX Link
    @inproceedings{Hagen-Eggert2011,
       author = {Hagen-Eggert, M. and Hillmann, D. and Koch, P. and Huttmann, G.},
       title = {Diffusion-sensitive Fourier-domain optical coherence tomography},
       editor = {James, G. Fujimoto and Joseph, A. Izatt and Valery, V. Tuchin},
       publisher = {SPIE},
       volume = {7889},
       pages = {78892B},
    URL = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/7889/78892B/Diffusion-sensitive-Fourier-domain-optical-coherencetomography/10.1117/12.873815.short},
    Year = { 2011}
    
    }
  • Bonin, Tim and Koch, Peter and Huttmann, Gereon: Comparison of fast swept source full-field OCT with conventional scanning OCT. no. 8091, pp. 80911K, SPIE, 2011
    BibTeX
    @inproceedings{Bonin2011,
       author = {Bonin, Tim and Koch, Peter and Huttmann, Gereon},
       title = {Comparison of fast swept source full-field OCT with conventional scanning OCT},
       editor = {Rainer, A. Leitgeb and Brett, E. Bouma},
       publisher = {SPIE},
       volume = {8091},
       pages = {80911K},
    year = { 2011}
    
    }
  • Theisen-Kunde, D. and Tedsen, S. and Doehn, C. and Jocham, D. and Kausch von Schmeling, I.: Comparison between a 1.92-mum fiber laser and a standard HF-dissection device for nephron-sparing kidney resection in a porcine in vivo study. Lasers Med Sci, no. 26, pp. 509-14, 2011
    BibTeX
    @article{Theisen-Kunde,
       author = {Theisen-Kunde, D. and Tedsen, S. and Doehn, C. and Jocham, D. and Kausch von Schmeling, I.},
       title = {Comparison between a 1.92-mum fiber laser and a standard HF-dissection device for nephron-sparing kidney resection in a porcine in vivo study},
       journal = {Lasers Med Sci},
       volume = {26},
       number = {4},
       pages = {509-14},
       note = {Theisen-Kunde, Dirk
    Tedsen, Sonke
    Doehn, Christian
    Jocham, Dieter
    Kausch von Schmeling, Ingo
    England
    Lasers Med Sci. 2011 Jul;26(4):509-14. Epub 2011 Jan 18.},
       abstract = {Nephron-sparing surgery was performed in a porcine model with a 1.92-mum fiber laser dissection device in comparison to a standard high-frequency dissection device. In nine pigs, general anesthesia and a median laparotomy were performed to expose both kidneys. On six kidneys (three HF and three laser) a partial renal parenchyma resection of the lower pole without opening of the renal pelvis was performed (group A). On 12 kidneys (four HF and eight laser), a hemi nephrectomy with opening of the renal pelvis was performed (group B). Total resection time including hemostasis of the remaining tissue was 501 +/- 394 s in group "A-laser " vs. 176 +/- 139 s in group "A-HF". For the group "B", the total resection time was 1174 +/- 501 s (B laser) vs. 960 +/- 407 s (B-HF). Blood loss was 28 +/- 22 ml in group "A laser " vs. 15 +/- 15 ml in group "A-HF". In group "B", the blood loss was 98 +/- 73 ml (B laser) vs. 137 +/- 118 ml (B-HF). No ischemic time for the kidneys was needed in group "A" for both dissection devices. In group "B", ischemia of the kidneys was performed three times during the eight laser procedures (420 +/- 60 s) and only once at the four HF procedures (1,260 s). Healing process was observed over 4-6 weeks, survival rate was 100%, and no renal fistulas were found after the survival period. In conclusion, no significant differences were found between the compared dissection devices. However, the laser system with the flexible transmission fiber may have an advantage for a laparoscopic approach by steerable instruments.},
       keywords = {Animals
    Carcinoma, Renal Cell/*surgery
    Hemostasis
    Kidney/*surgery
    Kidney Neoplasms/*surgery
    Laser Therapy/*instrumentation/methods
    Swine},
       year = {2011}
    }
  • Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.: Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy. Laser Physics, no. 21, pp. 130-136, 2011
    BibTeX
    @article{Wang,
       author = {Wang, J. and Li, Z. and Yao, C. and Xue, F. and Zhang, Z. and Hüttmann, G.},
       title = {Brownian diffusion of gold nanoparticles in an optical trap studied by fluorescence correlation spectroscopy},
       journal = {Laser Physics},
       volume = {21},
       number = {1},
       pages = {130-136},
       keywords = {Physik und Astronomie},
       year = {2011}
    }
  • Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi: A Case of Retinal Injury By A Violet Light-Emitting Diode. Retinal Cases and Brief Reports, no. 5, pp. 223-226 10.1097/ICB.0b013e3181e180d5, 2011
    BibTeX
    @article{Obana,
       author = {Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi},
       title = {A Case of Retinal Injury By A Violet Light-Emitting Diode},
       journal = {Retinal Cases and Brief Reports},
       volume = {5},
       number = {3},
       pages = {223-226 10.1097/ICB.0b013e3181e180d5},
       abstract = {Purpose: To describe the first case of retinal injury by a misuse of a toy using light-emitting diode. Methods: A 15-year-old male Japanese student received irradiation on his right eye by a 5 mW light-emitting diode of 410 nm wavelength for 20 seconds in 2 days. He noticed decreased vision and central scotoma approximately 2 weeks later from these events. The mechanism of injury was evaluated from the estimated irradiance on the retina by comparison with experimental threshold data published. Results: Chorioretinal atrophy with visual loss and central scotoma has remained on the fovea. The patient received an estimated dose of 1.58 J/cm2 2 times, which was close to the experimentally determined radiant exposure for photochemical injury of rat retina. Conclusion: The violet light from light-emitting diodes is a potential hazard for the retina, and thus, direct viewing into the beam should be avoided. Children, especially, should not be allowed to play with such toys without being carefully instructed about their proper use and fully supervised.},
       keywords = {black light
    light-emitting diode
    photochemical damage
    retinal injury
    visual disturbance.
    01271216-201100530-00011},
       year = {2011}
    }
    
  • Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi: A Case of Retinal Injury By A Violet Light-Emitting Diode. Retinal Cases and Brief Reports, no. 5, pp. 223-226 10.1097/ICB.0b013e3181e180d5, 2011
    BibTeX
    @article{Obana,
       author = {Obana, Akira and Brinkmann, Ralf and Gohto, Yuko and Nishimura, Kasumi},
       title = {A Case of Retinal Injury By A Violet Light-Emitting Diode},
       journal = {Retinal Cases and Brief Reports},
       volume = {5},
       number = {3},
       pages = {223-226 10.1097/ICB.0b013e3181e180d5},
       abstract = {Purpose: To describe the first case of retinal injury by a misuse of a toy using light-emitting diode. Methods: A 15-year-old male Japanese student received irradiation on his right eye by a 5 mW light-emitting diode of 410 nm wavelength for 20 seconds in 2 days. He noticed decreased vision and central scotoma approximately 2 weeks later from these events. The mechanism of injury was evaluated from the estimated irradiance on the retina by comparison with experimental threshold data published. Results: Chorioretinal atrophy with visual loss and central scotoma has remained on the fovea. The patient received an estimated dose of 1.58 J/cm2 2 times, which was close to the experimentally determined radiant exposure for photochemical injury of rat retina. Conclusion: The violet light from light-emitting diodes is a potential hazard for the retina, and thus, direct viewing into the beam should be avoided. Children, especially, should not be allowed to play with such toys without being carefully instructed about their proper use and fully supervised.},
       keywords = {black light
    light-emitting diode
    photochemical damage
    retinal injury
    visual disturbance.
    01271216-201100530-00011},
       year = {2011}
    }
    
  • Alex, A. and Weingast, J. and Hofer, B. and Eibl, M. and Binder, M. and Pehamberger, H. and Drexler, W. and Považay, B.: 3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers. Imaging in Medicine, no. 3, pp. 653-674, 2011
    BibTeX Link
    @article{Alex2011,
       author = {Alex, A. and Weingast, J. and Hofer, B. and Eibl, M. and Binder, M. and Pehamberger, H. and Drexler, W. and Považay, B.},
       title = {3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers},
       journal = {Imaging in Medicine},
       volume = {3},
       number = {6},
       keyword = {cancer diagnosis, contrast-enhanced imaging, dermatology, functional imaging,microscopy, multimodal imaging, optical coherence tomography, optical imaging, tomography},
      abstract = {High-resolution frequency domain optical coherence tomography (OCT) stands out amongst a range of novel dermatologic imaging technologies, with its good detection sensitivity around-100 dB, high measurement speeds allowing real-time image acquisition and its ability to acquire high definition cross-sectional and 3D tomograms of regions greater than 1 cm2, providing tissue information comparable to conventional histopathology without the need for any contrast agents. Typical axial and transverse resolutions of state-of-the-art OCT systems range between 1-10 m and approximately 20 m, respectively, depending on the employed wavelength region. This review investigates the significant progress accomplished in the field of dermatologic OCT with respect to other in vivo diagnostic methods for pre-excisional imaging of nonmelanoma skin cancers and specifically emphasizes state-of-the-art results achieved in different clinical pilot studies. Further technological extensions of OCT, various multimodal imaging approaches as well as potential clinical dermatologic applications are discussed. © 2011 Future Medicine Ltd.},
       pages = {653-674},
      ,
       url = {https://www.openaccessjournals.com/articles/3d-optical-coherence-tomography-for-clinical-diagnosis-of-nonmelanoma-skin-cancers-9179.html},
       year = {2011},
    keywords = {AG-Huber, OCT},
       type = {Journal Article}
    }